Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0002698

Physical Location & Seq

Genomic Coordinates Reverse (-)

21191108 .. 21197923

Gene Structure 6816 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG227700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGGCCGCAATGGATACATCAATTGCCCTTCAGCCTATTACGCAGGAAGCTGTTGATCTGGAGAACATCCCTGTGGAGGAAGTTTTTGAACAATTGAAATGTACAGCAGAGGGTCTCAATTCTGAGGAGGTGCAAAAGCGTCTGGAGGTTTTTGGTTTCAACAAACTTCAAGAGAAAAAGGAAAGTAAAATACTTAACTTTCTGGGGTTCATGTGGAACCCTTTGTCTTGGGTTATGGAAGCAGCTGCAATAATGTCAATAACTCTCGCATATGGAGGCGGGAGAAAAAGAGACGTTGAGGATTTCTGTGGAATCCTAGCACTACTCCTTATCAATTCAACAATAAGCTTCATCGAGGAAAACAATGCTGGAAATGCAGCTGCAGCGCTTATGGCCCGGCTTGCTCCAAAAGCCAAGGTTTTACGCGATGGGAAATGGAATGAGGAAGATGCAGCTGTGCTGGTTCCTGGAGACATTATTAGTATCAAGTTAGGAGATATAATTCCTGCTGATGCTCGCCTGCTTCAAGGAGATCCTTTGAAGATCGACCAGTCTGCTCTTACAGGAGAGTCTCTCCCGGTAACTAAGCATGCCGGTGAAGGAGTATACTCTGGCTCAACATGTAAACAAGGTGAAATTGAAGCAGTTGTCATCGCAACAGGAGTACATACCTTCTTTGGAAAAGCTGCTCATCTTGTGGAAAACACAACACATGTTGGGCACTTTCAGAAGGTCTTAACAGCCATTGGAAACTTTTGCATCTGCTCAATTGCCATAGGTATTGTGATTGAACTAATTGTCCTAGTTCGTCAAGGAAAGAGTTATCGTTCTAAAATAGATAACCTTCTTGTCCTACTTATTGGCGGCATCCCAATTGCTATGCCAACTGTTCTCTCTGTCACCATGGCCATTGGTTCTCATCGTTTGTCTCAGCAGGGTGCAATCACAAAGAGAATGACAGCCATTGAAGAAATGGCTGGGATGGATGTGCTCTGCAGTGATAAAACAGGAACTTTAACTCTCAACAAGTTAACAGTGGACAAGAATCTGATAGAGGTGTTTGCTAAAGATGTTGAAAAAGACTCGGTTGTATTGATGGCTGCAAGAGCTTCAAGGTTGGAAAATCAAGATGCCATTGATGCAGCAATTGTTGCAATGCTGGCTGATTCTAAGGAGGCACGAGAGGGAATTACAGAAGTTCACTTCCTTCCTTTCAATCCAACTGATAAGAGGACAGCACTTACATACCTAGACAAAGCTGGTAAAATGCACAGAGTGAGCAAAGGTGCACCAGAGCAGATACTCAATTTGGCATGGAACAAATCAGATATTGAAAAGAGGGCACACACAGTAATTGACAAATTTGCAGAACGTGGTCTTCGATCCCTTGCTGTTGCACGACAGGAAGTACCTGCTGGAACCAAAGACAGTCCTGGTGGACCTTGGGAGTTTGTTGGGCTTCTACCTTTGTTTGATCCACCACGTCACGACAGTGCTGAAACTATTAGAAGAGCTCTAGATCTTGGTGTGAGTGTTAAGATGATTACAGGCGATCAACTGGCAATTGCCAAAGAGACAGGGAGACGACTTGGGATGGGCTCAAACATGTATCCATCATCTTCATTGCTTGGTGAAAATAAGAATGGCTTAGATGCAAGTCTACAAATTGATGAACTCATTGAGAATGCTGATGGTTTTGCTGGTGTCTTTCCAGAGCATAAATATGAGATCGTTCAGCGATTACAAATTAAGAAGCACATAGTTGGAATGACCGGTGATGGAGTTAATGATGCACCTGCCTTGAAGAAAGCAGACATAGGAATTGCTGTGGCAGATGCCACAGATGCTGCTCGTAGTGCATCTGATATAGTACTGACAGAGCCTGGATTGAGTGTTATTATTAGTGCTGTTCTGACAAGCCGTGCAATATTTCAAAGAATGAAGAACTACACAATATATGCAGTGTCTATAACAATACGTATTGTGCTTGGTTTCTTGATGCTAACTGCATTCTGGCGATTTGATTTCCCTCCTTTTATGGTCCTCATCATAGCCATTCTTAATGACGGTACTATTATGACAATATCCAAAGACAAGGTTAAGCCATCTCCAGTTCCTGACAGTTGGAAGCTCAGTGAAATATTTGCAACTGGGATTGTCTTAGGGAGTTACCTTGCCGTGACCACAGCTATATTTTTCTATATCATTTACGAAACCCACTTCTTCCCGAAACACTTCCATCTAGACCTGGACTTCAAGAAACTCGCTGATCTCAATGAGTCGAGTTATACAGAGACACTAAATGGCCAGCTAGCATCTGTTGTGTACCTTCAAGTTAGCACCATCAGCCAGGCTCTAATTTTTGTAACTCGCTCCAGAGGTTGGTCATTCATGGAAAGACCTGGTCTTCTTCTTGTTGGTGCCTTCATAGTTGCTCAAATGGTTGCAACAGTAATATCTGCTCAGGCAACATGGAAATTTGCTCAAATTCATAGCGTTGGCTGGGGTTGGACTGGAGCAGTATGGATTTACAACATTATAATCTATATGCTTCTTGATCCTATCAAGTTTTTTGTGCGATATGCACTCAGTGGGAGGGCCTGGAGTTTGGTACTGAACAAAAGGACGGCTTTCACCACCCAAAAGGACTTTGGTAGGGAAGCTCGTGAGGCTGCATGGGCAACAGAACAGCGTACACTTCATGGCCTCCCAACTATGGACAAAAATATCTTCCATGAGAGGCACACATTCAGGGATGTTAGCATGATGGCTGAAGAAGCCCGACGACGTGCAGAGATTGCAAGACTAAGGGAAATTCACACTCTCAAAGGGAAGGTAGAGTCCTTTGCAAAGCTAAGGGGTTTGGACATTGACGTCAATCCACATTACACAGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

965

Amino Acids

106.17

Weight (kDa)

6.53

Isoelectric Point (pI)

38.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	22 - 86	1.3e-15	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	134 - 234	7.4e-31	P-type ATPase actuator domain
Hydrolase	PF00702	328 - 605	6.3e-15	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002698)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60330 AT3G60330 AT3G60330 AT3G60330
fragaria_vesca	FvH4_5g19790 FvH4_7g09980
malus_domestica	MD02G1216000.v1.1 MD03G1197700.v1.1 MD03G1197800.v1.1 MD04G1058300.v1.1
prunus_persica	Prupe.2G128800_v2.0.a1 Prupe.5G062200_v2.0.a1
pyrus_communis	pycom04g05210
rosa_chinensis	RchiOBHm_Chr1g0347581 RchiOBHm_Chr4g0412601 RchiOBHm_Chr7g0204571
rosa_laevigata	RLG00000003448 RLG00000028736 RLG00000030415
rosa_multiflora	Rmu_sc0001211.1_g000115 Rmu_sc0005397.1_g000001 Rmu_sc0007327.1_g000001 Rmu_sc0021384.1_g000002 Rmu_sc0024460.1_g000001 Rmu_sc0027638.1_g000006
rosa_roxburghii	Rroxscaffold_3G00252980 Rroxscaffold_4G00307640 Rroxscaffold_4G00307710
rosa_rugosa	Rorug01G0188000 Rorug07G0085200
rosa_samantha	Rh1DG200900 Rh7AG214600 Rh7BG212100 Rh7CG227700 Rh7DG222300
rosa_wichuraiana	Rw0G013650 Rw0G018200 Rw1G017220 Rw7G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2540
AarI	CACCTGC	1 cut(s)	1804
AasI	GACNNNNNNGTC	1 cut(s)	2873
AatII	GACGTC	1 cut(s)	2877
Acc36I	ACCTGC	2 cut(s)	1420, 1804
AccB1I	GGYRCC	1 cut(s)	2420
AccB7I	CCANNNNNTGG	1 cut(s)	2382
AccI	GTMKAC	2 cut(s)	606, 1660
AccII	CGCG	1 cut(s)	426
AciI	CCGC	3 cut(s)	6, 279, 864
AclWI	GGATC	4 cut(s)	527, 1377, 1469, 2552
AcoI	YGGCCR	3 cut(s)	3, 906, 2305
AcsI	RAATTY	4 cut(s)	1361, 2477, 2487, 2814
AcuI	CTGAAG	2 cut(s)	14, 2793
AcyI	GRCGYC	1 cut(s)	2874
AdeI	CACNNNGTG	2 cut(s)	1276, 2591
AfaI	GTAC	8 cut(s)	103, 666, 1410, 1872, 2071, 2327, 2613, 2695
AfeI	AGCGCT	1 cut(s)	387
AfiI	CCNNNNNNNGG	3 cut(s)	76, 2382, 2716
AflIII	ACRYGT	3 cut(s)	620, 712, 1605
AgeI	ACCGGT	1 cut(s)	1772
AjiI	CACGTC	2 cut(s)	1484, 2789
AjnI	CCWGG	7 cut(s)	466, 1432, 1882, 2247, 2349, 2401, 2600
AleI	CACNNNNGTG	1 cut(s)	1491
AloI	GAACNNNNNNTCC	2 cut(s)	783, 815
Alw21I	GWGCWC	3 cut(s)	993, 1291, 1516
Alw26I	GTCTC	8 cut(s)	119, 285, 465, 576, 933, 1568, 1576, 2288
Alw44I	GTGCAC	1 cut(s)	1287
AlwI	GGATC	4 cut(s)	527, 1377, 1469, 2552
AlwNI	CAGNNNCTG	3 cut(s)	1847, 1883, 2117
Aor51HI	AGCGCT	1 cut(s)	387
AoxI	GGCC	6 cut(s)	3, 393, 906, 2305, 2598, 2704
ApaLI	GTGCAC	1 cut(s)	1287
ApoI	RAATTY	4 cut(s)	1361, 2477, 2487, 2814
AsiGI	ACCGGT	1 cut(s)	1772
Asp700I	GAANNNNTTC	1 cut(s)	2234
AspLEI	GCGC	1 cut(s)	388
AspS9I	GGNCC	4 cut(s)	394, 1439, 2041, 2598
AsuC2I	CCSGG	2 cut(s)	397, 578
AsuHPI	GGTGA	6 cut(s)	608, 644, 892, 1643, 1787, 2626
AsuNHI	GCTAGC	1 cut(s)	2311
AvaII	GGWCC	2 cut(s)	1439, 2041
BaeGI	GKGCMC	3 cut(s)	723, 1291, 1345
BalI	TGGCCA	2 cut(s)	908, 2307
BanI	GGYRCC	1 cut(s)	2420
BanII	GRGCYC	2 cut(s)	1516, 1601
BauI	CACGAG	2 cut(s)	1179, 2664
BbsI	GAAGAC	2 cut(s)	1370, 2399
Bbv12I	GWGCWC	3 cut(s)	993, 1291, 1516
BceAI	ACGGC	3 cut(s)	1905, 2162, 2643
BcgI	CGANNNNNNTGC	2 cut(s)	536, 570
BciT130I	CCWGG	7 cut(s)	468, 1434, 1884, 2249, 2351, 2403, 2602
BciVI	GTATCC	2 cut(s)	7, 1620
BcnI	CCSGG	2 cut(s)	397, 578
BcoDI	GTCTC	8 cut(s)	119, 285, 465, 576, 933, 1568, 1576, 2288
BfaI	CTAG	6 cut(s)	317, 803, 1250, 1517, 2243, 2312
BfmI	CTRYAG	2 cut(s)	381, 994
BfoI	RGCGCY	1 cut(s)	389
BfuAI	ACCTGC	2 cut(s)	1420, 1804
BfuI	GTATCC	2 cut(s)	7, 1620
BglII	AGATCT	1 cut(s)	1519
BmcAI	AGTACT	1 cut(s)	1872
Bme1390I	CCNGG	9 cut(s)	397, 468, 578, 1434, 1884, 2249, 2351, 2403, 2602
Bme18I	GGWCC	2 cut(s)	1439, 2041
BmgBI	CACGTC	2 cut(s)	1484, 2789
BmgT120I	GGNCC	4 cut(s)	394, 1439, 2041, 2598
BmiI	GGNNCC	4 cut(s)	218, 465, 1420, 2422
BmrFI	CCNGG	9 cut(s)	397, 468, 578, 1434, 1884, 2249, 2351, 2403, 2602
BmrI	ACTGGG	1 cut(s)	2160
BmtI	GCTAGC	1 cut(s)	2315
BmuI	ACTGGG	1 cut(s)	2160
BpiI	GAAGAC	2 cut(s)	1370, 2399
BplI	GAGNNNNNCTC	2 cut(s)	558, 590
BpmI	CTGGAG	7 cut(s)	80, 164, 489, 2094, 2359, 2535, 2623
Bpu10I	CCTNAGC	2 cut(s)	2463, 2855
BpuMI	CCSGG	2 cut(s)	397, 578
BsaAI	YACGTR	1 cut(s)	1979
BsaHI	GRCGYC	1 cut(s)	2874
BsaI	GGTCTC	1 cut(s)	119
BsaJI	CCNNGG	3 cut(s)	414, 903, 1442
BsaWI	WCCGGW	1 cut(s)	1772
Bsc4I	CCNNNNNNNGG	3 cut(s)	76, 2382, 2716
Bse118I	RCCGGY	2 cut(s)	593, 1772
Bse1I	ACTGG	5 cut(s)	550, 1563, 2111, 2155, 2518
Bse3DI	GCAATG	3 cut(s)	15, 1161, 1622
BseBI	CCWGG	7 cut(s)	468, 1434, 1884, 2249, 2351, 2403, 2602
BseDI	CCNNGG	3 cut(s)	414, 903, 1442
BseGI	GGATG	6 cut(s)	66, 867, 987, 991, 1599, 2761
BseLI	CCNNNNNNNGG	3 cut(s)	76, 2382, 2716
BseMI	GCAATG	3 cut(s)	15, 1161, 1622
BseMII	CTCAG	5 cut(s)	114, 944, 2146, 2477, 2602
BseNI	ACTGG	5 cut(s)	550, 1563, 2111, 2155, 2518
BseRI	GAGGAG	1 cut(s)	140
BseSI	GKGCMC	3 cut(s)	723, 1291, 1345
BseYI	CCCAGC	2 cut(s)	977, 2502
BsgI	GTGCAG	1 cut(s)	2811
Bsh1236I	CGCG	1 cut(s)	426
BshFI	GGCC	6 cut(s)	5, 395, 908, 2307, 2600, 2706
BshNI	GGYRCC	1 cut(s)	2420
BshTI	ACCGGT	1 cut(s)	1772
BsiHKAI	GWGCWC	3 cut(s)	993, 1291, 1516
BsiSI	CCGG	4 cut(s)	397, 578, 594, 1773
BslI	CCNNNNNNNGG	3 cut(s)	76, 2382, 2716
BsmAI	GTCTC	8 cut(s)	119, 285, 465, 576, 933, 1568, 1576, 2288
BsmBI	CGTCTC	2 cut(s)	285, 1576
BsmI	GAATGC	2 cut(s)	1690, 2009
BsnI	GGCC	6 cut(s)	5, 395, 908, 2307, 2600, 2706
Bso31I	GGTCTC	1 cut(s)	119
Bsp1286I	GDGCHC	6 cut(s)	723, 993, 1291, 1345, 1516, 1601
Bsp1407I	TGTACA	1 cut(s)	101
Bsp19I	CCATGG	1 cut(s)	903
BspACI	CCGC	3 cut(s)	6, 279, 864
BspANI	GGCC	6 cut(s)	5, 395, 908, 2307, 2600, 2706
BspCNI	CTCAG	5 cut(s)	115, 943, 2145, 2476, 2601
BspFNI	CGCG	1 cut(s)	426
BspLI	GGNNCC	4 cut(s)	218, 465, 1420, 2422
BspMAI	CTGCAG	2 cut(s)	385, 998
BspMI	ACCTGC	2 cut(s)	1420, 1804
BspOI	GCTAGC	1 cut(s)	2315
BspPI	GGATC	4 cut(s)	527, 1377, 1469, 2552
BspQI	GCTCTTC	1 cut(s)	1504
BspT107I	GGYRCC	1 cut(s)	2420
BspTNI	GGTCTC	1 cut(s)	119
BsrDI	GCAATG	3 cut(s)	15, 1161, 1622
BsrFI	RCCGGY	2 cut(s)	593, 1772
BsrGI	TGTACA	1 cut(s)	101
BsrI	ACTGG	5 cut(s)	550, 1563, 2111, 2155, 2518
BssAI	RCCGGY	2 cut(s)	593, 1772
BssECI	CCNNGG	3 cut(s)	414, 903, 1442
BssNAI	GTATAC	1 cut(s)	607
BssNI	GRCGYC	1 cut(s)	2874
BssSI	CACGAG	2 cut(s)	1179, 2664
BssT1I	CCWWGG	3 cut(s)	414, 903, 1442
Bst1107I	GTATAC	1 cut(s)	607
Bst2BI	CACGAG	2 cut(s)	1179, 2664
Bst2UI	CCWGG	7 cut(s)	468, 1434, 1884, 2249, 2351, 2403, 2602
Bst4CI	ACNGT	9 cut(s)	889, 1036, 1351, 1430, 1493, 2069, 2123, 2452, 2893
Bst6I	CTCTTC	1 cut(s)	1504
BstACI	GRCGYC	1 cut(s)	2874
BstAPI	GCANNNNNTGC	2 cut(s)	1151, 2798
BstAUI	TGTACA	1 cut(s)	101
BstBAI	YACGTR	1 cut(s)	1979
BstC8I	GCNNGC	7 cut(s)	402, 517, 521, 591, 1161, 2309, 2313
BstDSI	CCRYGG	1 cut(s)	903
BstF5I	GGATG	6 cut(s)	66, 867, 987, 991, 1599, 2761
BstFNI	CGCG	1 cut(s)	426
BstH2I	RGCGCY	1 cut(s)	389
BstHHI	GCGC	1 cut(s)	388
BstMAI	GTCTC	8 cut(s)	119, 285, 465, 576, 933, 1568, 1576, 2288
BstNI	CCWGG	7 cut(s)	468, 1434, 1884, 2249, 2351, 2403, 2602
BstNSI	RCATGY	4 cut(s)	593, 624, 716, 1609
BstSCI	CCNGG	9 cut(s)	395, 466, 576, 1432, 1882, 2247, 2349, 2401, 2600
BstSFI	CTRYAG	2 cut(s)	381, 994
BstSLI	GKGCMC	3 cut(s)	723, 1291, 1345
BstSNI	TACGTA	1 cut(s)	1979
BstUI	CGCG	1 cut(s)	426
BstV2I	GAAGAC	2 cut(s)	1370, 2399
BstX2I	RGATCY	2 cut(s)	532, 1519
BstYI	RGATCY	2 cut(s)	532, 1519
BstZ17I	GTATAC	1 cut(s)	607
BsuI	GTATCC	2 cut(s)	7, 1620
BsuRI	GGCC	6 cut(s)	5, 395, 908, 2307, 2600, 2706
BtgI	CCRYGG	1 cut(s)	903
BtgZI	GCGATG	2 cut(s)	441, 637
BtrI	CACGTC	2 cut(s)	1484, 2789
BtsCI	GGATG	6 cut(s)	66, 867, 987, 991, 1599, 2761
BtsI	GCAGTG	2 cut(s)	1003, 1968
BtsIMutI	CAGTG	6 cut(s)	1003, 1041, 1498, 1968, 2140, 2596
BveI	ACCTGC	2 cut(s)	1420, 1804
Cac8I	GCNNGC	7 cut(s)	402, 517, 521, 591, 1161, 2309, 2313
CaiI	CAGNNNCTG	3 cut(s)	1847, 1883, 2117
CfoI	GCGC	1 cut(s)	388
Cfr10I	RCCGGY	2 cut(s)	593, 1772
Cfr13I	GGNCC	4 cut(s)	394, 1439, 2041, 2598
CseI	GACGC	1 cut(s)	128
CsiI	ACCWGGT	1 cut(s)	2401
Csp6I	GTAC	8 cut(s)	102, 665, 1409, 1871, 2070, 2326, 2612, 2694
CspAI	ACCGGT	1 cut(s)	1772
CviQI	GTAC	8 cut(s)	102, 665, 1409, 1871, 2070, 2326, 2612, 2694
DraIII	CACNNNGTG	2 cut(s)	1276, 2591
DrdI	GACNNNNNNGTC	1 cut(s)	2873
DseDI	GACNNNNNNGTC	1 cut(s)	2873
EaeI	YGGCCR	3 cut(s)	3, 906, 2305
Eam1104I	CTCTTC	1 cut(s)	1504
EarI	CTCTTC	1 cut(s)	1504
Ecl136II	GAGCTC	1 cut(s)	1514
Eco105I	TACGTA	1 cut(s)	1979
Eco130I	CCWWGG	3 cut(s)	414, 903, 1442
Eco24I	GRGCYC	2 cut(s)	1516, 1601
Eco31I	GGTCTC	1 cut(s)	119
Eco47I	GGWCC	2 cut(s)	1439, 2041
Eco47III	AGCGCT	1 cut(s)	387
Eco53kI	GAGCTC	1 cut(s)	1514
Eco57I	CTGAAG	2 cut(s)	14, 2793
EcoICRI	GAGCTC	1 cut(s)	1514
EcoO109I	RGGNCCY	1 cut(s)	2598
EcoRII	CCWGG	7 cut(s)	466, 1432, 1882, 2247, 2349, 2401, 2600
EcoT14I	CCWWGG	3 cut(s)	414, 903, 1442
EcoT38I	GRGCYC	2 cut(s)	1516, 1601
ErhI	CCWWGG	3 cut(s)	414, 903, 1442
Esp3I	CGTCTC	2 cut(s)	285, 1576
FalI	AAGNNNNNCTT	4 cut(s)	1631, 1663, 2614, 2646
FauI	CCCGC	1 cut(s)	272
FauNDI	CATATG	1 cut(s)	271
FblI	GTMKAC	2 cut(s)	606, 1660
FokI	GGATG	6 cut(s)	53, 854, 994, 998, 1606, 2768
FriOI	GRGCYC	2 cut(s)	1516, 1601
FspBI	CTAG	6 cut(s)	317, 803, 1250, 1517, 2243, 2312
GlaI	GCGC	1 cut(s)	387
GsaI	CCCAGC	2 cut(s)	981, 2506
GsuI	CTGGAG	7 cut(s)	80, 164, 489, 2094, 2359, 2535, 2623
HaeII	RGCGCY	1 cut(s)	389
HaeIII	GGCC	6 cut(s)	5, 395, 908, 2307, 2600, 2706
HapII	CCGG	4 cut(s)	397, 578, 594, 1773
HgaI	GACGC	1 cut(s)	128
HhaI	GCGC	1 cut(s)	388
Hin1I	GRCGYC	1 cut(s)	2874
Hin6I	GCGC	1 cut(s)	386
HinP1I	GCGC	1 cut(s)	386
HincII	GTYRAC	1 cut(s)	1032
HindII	GTYRAC	1 cut(s)	1032
HindIII	AAGCTT	1 cut(s)	346
HinfI	GANTC	7 cut(s)	312, 569, 1045, 1082, 1166, 2279, 2840
HpaI	GTTAAC	1 cut(s)	1032
HpaII	CCGG	4 cut(s)	397, 578, 594, 1773
HphI	GGTGA	6 cut(s)	608, 644, 892, 1643, 1787, 2626
Hpy188I	TCNGA	6 cut(s)	124, 729, 1050, 1327, 1864, 1914
Hpy99I	CGWCG	2 cut(s)	2787, 2790
HpyAV	CCTTC	9 cut(s)	38, 593, 682, 724, 854, 1217, 2339, 2434, 2827
HpyCH4III	ACNGT	9 cut(s)	889, 1036, 1351, 1430, 1493, 2069, 2123, 2452, 2893
HpyCH4IV	ACGT	6 cut(s)	294, 1372, 1483, 1978, 2788, 2874
HpySE526I	ACGT	6 cut(s)	294, 1372, 1483, 1978, 2788, 2874
Hsp92I	GRCGYC	1 cut(s)	2874
HspAI	GCGC	1 cut(s)	386
KspAI	GTTAAC	1 cut(s)	1032
LguI	GCTCTTC	1 cut(s)	1504
LmnI	GCTCC	3 cut(s)	409, 2378, 2516
MabI	ACCWGGT	1 cut(s)	2401
MaeI	CTAG	6 cut(s)	317, 803, 1250, 1517, 2243, 2312
MaeII	ACGT	6 cut(s)	294, 1372, 1483, 1978, 2788, 2874
MaeIII	GTNAC	6 cut(s)	580, 898, 1484, 2168, 2179, 2365
MfeI	CAATTG	6 cut(s)	21, 92, 768, 873, 1146, 1563
MflI	RGATCY	2 cut(s)	532, 1519
MhlI	GDGCHC	6 cut(s)	723, 993, 1291, 1345, 1516, 1601
MlsI	TGGCCA	2 cut(s)	908, 2307
MluNI	TGGCCA	2 cut(s)	908, 2307
MlyI	GAGTC	4 cut(s)	578, 1076, 2288, 2849
MmeI	TCCRAC	5 cut(s)	1098, 1244, 1744, 2105, 2489
Mox20I	TGGCCA	2 cut(s)	908, 2307
MroXI	GAANNNNTTC	1 cut(s)	2234
MscI	TGGCCA	2 cut(s)	908, 2307
MseI	TTAA	9 cut(s)	195, 737, 1016, 1031, 1536, 1749, 1785, 2061, 2100
MslI	CAYNNNNRTG	4 cut(s)	71, 594, 1491, 1722
Msp20I	TGGCCA	2 cut(s)	908, 2307
MspA1I	CMGCKG	3 cut(s)	245, 380, 455
MspI	CCGG	4 cut(s)	397, 578, 594, 1773
MspR9I	CCNGG	9 cut(s)	397, 468, 578, 1434, 1884, 2249, 2351, 2403, 2602
MunI	CAATTG	6 cut(s)	21, 92, 768, 873, 1146, 1563
Mva1269I	GAATGC	2 cut(s)	1690, 2009
MvaI	CCWGG	7 cut(s)	468, 1434, 1884, 2249, 2351, 2403, 2602
MvnI	CGCG	1 cut(s)	426
NciI	CCSGG	2 cut(s)	397, 578
NcoI	CCATGG	1 cut(s)	903
NdeI	CATATG	1 cut(s)	271
NheI	GCTAGC	1 cut(s)	2311
NlaIV	GGNNCC	4 cut(s)	218, 465, 1420, 2422
NmuCI	GTSAC	3 cut(s)	898, 1484, 2179
NspI	RCATGY	4 cut(s)	593, 624, 716, 1609
OliI	CACNNNNGTG	1 cut(s)	1491
PaeI	GCATGC	1 cut(s)	593
PaqCI	CACCTGC	1 cut(s)	1804
PciI	ACATGT	3 cut(s)	620, 712, 1605
PciSI	GCTCTTC	1 cut(s)	1504
PcsI	WCGNNNNNNNCGW	1 cut(s)	1378
PctI	GAATGC	2 cut(s)	1690, 2009
PdmI	GAANNNNTTC	1 cut(s)	2234
PfeI	GAWTC	3 cut(s)	312, 1045, 1166
PflFI	GACNNNGTC	1 cut(s)	2403
PflMI	CCANNNNNTGG	1 cut(s)	2382
PfoI	TCCNGGA	1 cut(s)	466
PinAI	ACCGGT	1 cut(s)	1772
PleI	GAGTC	4 cut(s)	577, 1076, 2287, 2848
PpsI	GAGTC	4 cut(s)	577, 1076, 2287, 2848
Ppu21I	YACGTR	1 cut(s)	1979
PscI	ACATGT	3 cut(s)	620, 712, 1605
PsiI	TTATAA	1 cut(s)	2540
Psp124BI	GAGCTC	1 cut(s)	1516
Psp6I	CCWGG	7 cut(s)	466, 1432, 1882, 2247, 2349, 2401, 2600
PspFI	CCCAGC	2 cut(s)	977, 2502
PspGI	CCWGG	7 cut(s)	466, 1432, 1882, 2247, 2349, 2401, 2600
PspN4I	GGNNCC	4 cut(s)	218, 465, 1420, 2422
PspPI	GGNCC	4 cut(s)	394, 1439, 2041, 2598
PstI	CTGCAG	2 cut(s)	385, 998
PstNI	CAGNNNCTG	3 cut(s)	1847, 1883, 2117
PsuI	RGATCY	2 cut(s)	532, 1519
PsyI	GACNNNGTC	1 cut(s)	2403
PvuII	CAGCTG	3 cut(s)	245, 380, 455
RsaI	GTAC	8 cut(s)	103, 666, 1410, 1872, 2071, 2327, 2613, 2695
RsaNI	GTAC	8 cut(s)	102, 665, 1409, 1871, 2070, 2326, 2612, 2694
RseI	CAYNNNNRTG	4 cut(s)	71, 594, 1491, 1722
SacI	GAGCTC	1 cut(s)	1516
SapI	GCTCTTC	1 cut(s)	1504
SaqAI	TTAA	9 cut(s)	195, 737, 1016, 1031, 1536, 1749, 1785, 2061, 2100
Sau96I	GGNCC	4 cut(s)	394, 1439, 2041, 2598
ScaI	AGTACT	1 cut(s)	1872
SchI	GAGTC	4 cut(s)	578, 1076, 2288, 2849
ScrFI	CCNGG	9 cut(s)	397, 468, 578, 1434, 1884, 2249, 2351, 2403, 2602
SduI	GDGCHC	6 cut(s)	723, 993, 1291, 1345, 1516, 1601
SexAI	ACCWGGT	1 cut(s)	2401
SfcI	CTRYAG	2 cut(s)	381, 994
SinI	GGWCC	2 cut(s)	1439, 2041
SmiMI	CAYNNNNRTG	4 cut(s)	71, 594, 1491, 1722
SnaBI	TACGTA	1 cut(s)	1979
SphI	GCATGC	1 cut(s)	593
SsiI	CCGC	3 cut(s)	6, 279, 864
SspI	AATATT	2 cut(s)	1929, 2142
SspMI	CTAG	6 cut(s)	317, 803, 1250, 1517, 2243, 2312
SstI	GAGCTC	1 cut(s)	1516
StyD4I	CCNGG	9 cut(s)	395, 466, 576, 1432, 1882, 2247, 2349, 2401, 2600
StyI	CCWWGG	3 cut(s)	414, 903, 1442
TaaI	ACNGT	9 cut(s)	889, 1036, 1351, 1430, 1493, 2069, 2123, 2452, 2893
TaiI	ACGT	6 cut(s)	297, 1375, 1486, 1981, 2791, 2877
TaqI	TCGA	4 cut(s)	354, 546, 1381, 2282
TatI	WGTACW	3 cut(s)	101, 664, 1870
TauI	GCSGC	2 cut(s)	8, 867
TfiI	GAWTC	3 cut(s)	312, 1045, 1166
Tru1I	TTAA	9 cut(s)	195, 737, 1016, 1031, 1536, 1749, 1785, 2061, 2100
Tru9I	TTAA	9 cut(s)	195, 737, 1016, 1031, 1536, 1749, 1785, 2061, 2100
TscAI	CASTG	6 cut(s)	1003, 1041, 1498, 1968, 2140, 2596
TseFI	GTSAC	3 cut(s)	898, 1484, 2179
Tsp45I	GTSAC	3 cut(s)	898, 1484, 2179
TspDTI	ATGAA	9 cut(s)	199, 340, 1611, 1686, 1955, 2380, 2416, 2480, 2690
TspRI	CASTG	6 cut(s)	1003, 1041, 1498, 1968, 2140, 2596
Tth111I	GACNNNGTC	1 cut(s)	2403
Van91I	CCANNNNNTGG	1 cut(s)	2382
VneI	GTGCAC	1 cut(s)	1287
VpaK11BI	GGWCC	2 cut(s)	1439, 2041
XapI	RAATTY	4 cut(s)	1361, 2477, 2487, 2814
XbaI	TCTAGA	2 cut(s)	1516, 2242
XceI	RCATGY	4 cut(s)	593, 624, 716, 1609
XcmI	CCANNNNNNNNNTGG	2 cut(s)	970, 2648
XmiI	GTMKAC	2 cut(s)	606, 1660
XmnI	GAANNNNTTC	1 cut(s)	2234
XspI	CTAG	6 cut(s)	317, 803, 1250, 1517, 2243, 2312
ZraI	GACGTC	1 cut(s)	2875
ZrmI	AGTACT	1 cut(s)	1872

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.