Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002698

Physical Location & Seq

Genomic Coordinates Forward (+)

24521351 .. 24526915

Gene Structure 5565 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1216000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2976 bp

ATGGTGGTGGCCGAGTCGGTTGCCCTCGACGCAATCATCAAGGAAGCTGTTGATCTGGAGAATGTCCCGCTGAACGAAGTTTTTGACCATCTGAAATGCACGAGAGATGGTTTAAGCTCCGATGAAGTCCAAGAGCGGTTGGATTTATTTGGTTACAATAAGCTTGAAGAAAAAAAGGAAAGTAAAATCTTGAAGTTTTTGGGATTTATGTGGAATCCTCTGTCCTGGGTCATGGAAGCTGCAGCTATCATAGCCGTTGCTTTCACACAAGGAGGGTCACAACCAGCAGATTATCATGATTTTGTCGGCATCCTCGTCCTGCTCATTATCAATTCTACCATCAGTTTTATAGAAGAAAACAATGCAGGCAATGCGGCAGCTGCTCTTATGGCTCGTTTGGCCCCAAAAGCAAAGGTCTTGCGCAATGGAAAATGGGCGGAAGAAGATGCTGCTGTATTGGTTCCTGGAGACATAATTAGCATCAAACTTGGTGATATCGTTCCTGCTGATGCCCGTCTTCTTGAAGGAGATCCTTTAAAGATTGATCAGTCTGCTCTTACTGGAGAATCGCTTCCGGTGACCAAAAATCCTGGCGATGGAGTTTACTCGGGATCAACATGCAAGCAGGGAGAGATTGAAGGGGTTGTTATTGCAACTGGAGTTCACACTTTCTTTGGAAAGGCAGCTCATCTTGTCGAGAACACAACACATGTTGGCCATTTCCAGCAGGTATTAACGTCAATTGGAAACTTTTGCATTTGTTCCATCGCTGTTGGGATGGCAATTGAAATCATAGTAATATACGGGATTCATGGGAGGGAATACCGTACGGGTATTGATAACCTTCTCGTCCTCCTAATCGGTGGGATCCCTATTGCTATGCCAACTGTTCTTTCTGTCACAATGGCCATCGGCTCACATCGTTTGTCCCAGCAGGGTGCCATAACAAAGAGAATGACTGCCATTGAAGAAATGGCAGGGATGGATGTTCTATGCAGTGATAAAACAGGAACACTGACACTTAACAAGCTTACAGTGGACAAAAATATGATTGAGGTTTTTACCAAAGGCGTTGACAAGGATCAGGTTATGCTTATGGCTGCAAGAGCATCAAGGCTCGAAAATCAAGATGCTATTGATACTGCAATTGTTTCGATGTTGGGAGACCCTAAGGAGGCACGCGCCGGAATCAGAGAAATTCACTTCCTTCCATTCAATCCTACCGATAAAAGAACTGCACTCACATACATTGATGCCGCTGGTAAAATGCACAGGGTCAGCAAAGGTGCACCTGAACAGATTCTTAATCTGGCACATAACAAATCAGCAATTGAGAAGAAGGTACACGCAATGATTGATAAGTTTGCAGAACGTGGACTACGATCCCTTGGTGTTGCCCTCCAGGAAGTACCTGCTGGAACCAAAGACAATCCTGGTGGACCCTGGGAATTTGTTGGTCTTCTCCCTCTCTTCGACCCTCCTCGCCATGACAGTGCCGAGACAATCAGAAGAGCTCTGGATCTTGGTGTCAGCGTTAAAATGATCACTGGGGACCAACTTGCAATCGGTAAGGAAACAGGAAGGCGGCTTGGGATGGGTACAAACATGTATCCTTCTTCAGCTCTGCTTGGTGAAAGTAAGGATGGGGATCTTGCTACCGTTTCCGTTGATGAACTCATTGAAAAAGCCGATGGTTTTGCTGGCGTCTTTCCTGAGCATAAATACGAGATTGTGAAGAGATTACAAGCTAGAAAGCACATCTGTGGAATGACTGGTGACGGAGTGAATGATGCCCCGGCCTTAAAGAAAGCTGACATTGGAATTGCGGTAGCAGATTCCACAGATGCTGCACGTAGCGCTTCTGATATAGTTTTAACAGAACCTGGATTGAGTGTGATCATTAGTGCTGTGTTAACAAGCCGTGCAATTTTCCAGAGAATGAAAAACTATACGATATATGCTGTCTCGATCACCATTCGTATTGTGCTAGGTTTTATGTTGCTGGCTGTCTTCTGGAAATTCGACTTCCCTCCTTTTATGGTTCTTGTCATTGCTGTTCTTAACGATGGTACTATCATGACCATATCCAAAGATAGGGTCAAACCATCTCCATTTCCTGACAGTTGGAAGCTCAGTGAGATATTTGTAACTGGAATTGCCCTCGGTGGTTACCTTGGTCTAACTACTGTTTTGTTCTTCTGGATATCTTATGAAACCAACTTCTTTCCGGAAAAATTTAACGTGAGAGACTTCAATAAACACCATTTCAATATGAAAAATAAGGATGAAGCTGCTATACTAAAGGATCAGATGTCGTCAGCTTTGTATCTTCAAATCAGCACCATCAGCCAGGCCTTGATATTTGTCACTCGCTCCCGCGGTTGGTCTTTCCGTGAAAGACCTGGCTTTTTGCTTGTCATTGCCTTCATCATCGCTCAACTGGTACTGAATAGTTCCTGCCTCGGAAGTAATTCCAATGTTTATAAACATTTGTTGACATCCAATAATTATTTGCAGATTGCGACAGTTATATCCGCAACAGCAACGTGGAAATTTACAAAAATCAGAAGTATTGGTTGGGGCTGGACTGGTATAATTTGGATGTACAATATCGTCATTTACATGCTGCTTGACCCTATCAAATTCATTGTCCGTTACGCACTCAGTGGAAAGGCTTGGGGTTTAATAGTAGACCAAAGAACCGCATTTACCAGCAAAAACGACTTTGGGAAGGAAGCTCGTGAGGCTGCGTGGGCAACTGAACAACGAACGCTGCATGGCCTCCAATCTCGTGAAACAAAAAAGTTTACAGAGAAGGGCACTTTTAGGGACATTAATCTCATGGCCGAAGAAGCTAAAAGGCGGGCAGAGATTGCAAGGTTGAGGGAGCTTCACACTCTGAAGGGAAAGGTTGAGTCATTTGCCAAGCTGAAGGGTATAGACATTGATGCCATCAATCAAAACTACACCCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

992

Amino Acids

108.86

Weight (kDa)

7.06

Isoelectric Point (pI)

34.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	21 - 85	7.7e-15	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	133 - 233	2.2e-30	P-type ATPase actuator domain
Hydrolase	PF00702	328 - 605	4.2e-13	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002698)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60330 AT3G60330 AT3G60330 AT3G60330
fragaria_vesca	FvH4_5g19790 FvH4_7g09980
malus_domestica	MD02G1216000.v1.1 MD03G1197700.v1.1 MD03G1197800.v1.1 MD04G1058300.v1.1
prunus_persica	Prupe.2G128800_v2.0.a1 Prupe.5G062200_v2.0.a1
pyrus_communis	pycom04g05210
rosa_chinensis	RchiOBHm_Chr1g0347581 RchiOBHm_Chr4g0412601 RchiOBHm_Chr7g0204571
rosa_laevigata	RLG00000003448 RLG00000028736 RLG00000030415
rosa_multiflora	Rmu_sc0001211.1_g000115 Rmu_sc0005397.1_g000001 Rmu_sc0007327.1_g000001 Rmu_sc0021384.1_g000002 Rmu_sc0024460.1_g000001 Rmu_sc0027638.1_g000006
rosa_roxburghii	Rroxscaffold_3G00252980 Rroxscaffold_4G00307640 Rroxscaffold_4G00307710
rosa_rugosa	Rorug01G0188000 Rorug07G0085200
rosa_samantha	Rh1DG200900 Rh7AG214600 Rh7BG212100 Rh7CG227700 Rh7DG222300
rosa_wichuraiana	Rw0G013650 Rw0G018200 Rw1G017220 Rw7G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2484
Acc16I	TGCGCA	1 cut(s)	422
Acc36I	ACCTGC	2 cut(s)	718, 1420
AccB1I	GGYRCC	1 cut(s)	938
AccBSI	CCGCTC	1 cut(s)	136
AccI	GTMKAC	1 cut(s)	2691
AccII	CGCG	2 cut(s)	1182, 2379
AccIII	TCCGGA	1 cut(s)	2227
AclWI	GGATC	9 cut(s)	524, 619, 862, 875, 1089, 1377, 1527, 1656, 2313
AcoI	YGGCCR	4 cut(s)	9, 715, 906, 2844
AcsI	RAATTY	6 cut(s)	1197, 1448, 2018, 2234, 2552, 2642
AcuI	CTGAAG	3 cut(s)	1602, 2921, 2951
AcyI	GRCGYC	1 cut(s)	1704
AdeI	CACNNNGTG	1 cut(s)	2666
AfaI	GTAC	7 cut(s)	829, 1344, 1410, 1600, 2071, 2445, 2606
AfeI	AGCGCT	1 cut(s)	1858
AfiI	CCNNNNNNNGG	4 cut(s)	596, 1174, 2094, 2382
AflIII	ACRYGT	2 cut(s)	709, 1605
AjnI	CCWGG	9 cut(s)	224, 463, 589, 1401, 1432, 1442, 1882, 2349, 2401
AjuI	GAANNNNNNNTTGG	4 cut(s)	877, 909, 2560, 2592
AleI	CACNNNNGTG	1 cut(s)	1761
Alw21I	GWGCWC	2 cut(s)	1291, 1516
Alw26I	GTCTC	5 cut(s)	462, 1158, 1493, 1969, 2241
Alw44I	GTGCAC	1 cut(s)	1287
AlwI	GGATC	9 cut(s)	524, 619, 862, 875, 1089, 1377, 1527, 1656, 2313
AlwNI	CAGNNNCTG	2 cut(s)	1847, 1883
Ama87I	CYCGRG	1 cut(s)	607
Aor13HI	TCCGGA	1 cut(s)	2227
Aor51HI	AGCGCT	1 cut(s)	1858
AoxI	GGCC	8 cut(s)	9, 399, 715, 906, 1797, 2352, 2779, 2844
ApaLI	GTGCAC	1 cut(s)	1287
ApoI	RAATTY	6 cut(s)	1197, 1448, 2018, 2234, 2552, 2642
ArsI	GACNNNNNNTTYG	4 cut(s)	2082, 2114, 2634, 2666
AseI	ATTAAT	1 cut(s)	2835
Asp700I	GAANNNNTTC	3 cut(s)	570, 1299, 2470
AspLEI	GCGC	3 cut(s)	423, 1184, 1859
AspS9I	GGNCC	3 cut(s)	400, 1439, 1552
AsuC2I	CCSGG	1 cut(s)	1796
AsuHPI	GGTGA	5 cut(s)	503, 589, 1643, 1787, 1963
AvaI	CYCGRG	1 cut(s)	607
AvaII	GGWCC	2 cut(s)	1439, 1552
AxyI	CCTNAGG	1 cut(s)	1170
BaeGI	GKGCMC	2 cut(s)	1291, 2822
BalI	TGGCCA	2 cut(s)	717, 908
BamHI	GGATCC	1 cut(s)	867
BanI	GGYRCC	1 cut(s)	938
BanII	GRGCYC	1 cut(s)	1516
BarI	GAAGNNNNNNTAC	2 cut(s)	2280, 2312
BauI	CACGAG	3 cut(s)	100, 2739, 2790
BbsI	GAAGAC	3 cut(s)	509, 1451, 2002
Bbv12I	GWGCWC	2 cut(s)	1291, 1516
BceAI	ACGGC	2 cut(s)	239, 1905
BcgI	CGANNNNNNTGC	5 cut(s)	36, 1134, 1168, 1679, 1713
BciT130I	CCWGG	9 cut(s)	226, 465, 591, 1403, 1434, 1444, 1884, 2351, 2403
BciVI	GTATCC	1 cut(s)	1620
BclI	TGATCA	3 cut(s)	544, 1542, 1896
BcnI	CCSGG	1 cut(s)	1796
BcoDI	GTCTC	5 cut(s)	462, 1158, 1493, 1969, 2241
BfaI	CTAG	3 cut(s)	1749, 1988, 2974
BfmI	CTRYAG	1 cut(s)	240
BfoI	RGCGCY	1 cut(s)	1860
BfuAI	ACCTGC	2 cut(s)	718, 1420
BfuI	GTATCC	1 cut(s)	1620
Bme18I	GGWCC	2 cut(s)	1439, 1552
BmeT110I	CYCGRG	1 cut(s)	607
BmgT120I	GGNCC	3 cut(s)	400, 1439, 1552
BmiI	GGNNCC	7 cut(s)	402, 462, 869, 940, 1420, 1441, 1553
BmrI	ACTGGG	1 cut(s)	1557
BmuI	ACTGGG	1 cut(s)	1557
BpiI	GAAGAC	3 cut(s)	509, 1451, 2002
BpmI	CTGGAG	5 cut(s)	77, 486, 582, 678, 1385
Bpu10I	CCTNAGC	1 cut(s)	1713
BpuMI	CCSGG	1 cut(s)	1796
BsaAI	YACGTR	1 cut(s)	1853
BsaBI	GATNNNNATC	1 cut(s)	1647
BsaHI	GRCGYC	1 cut(s)	1704
BsaI	GGTCTC	1 cut(s)	1158
BsaJI	CCNNGG	9 cut(s)	225, 1387, 1442, 1443, 1794, 2161, 2173, 2377, 2461
BsaWI	WCCGGW	2 cut(s)	574, 2227
Bsc4I	CCNNNNNNNGG	4 cut(s)	596, 1174, 2094, 2382
Bse1I	ACTGG	7 cut(s)	565, 661, 1552, 1777, 2155, 2445, 2593
Bse21I	CCTNAGG	1 cut(s)	1170
Bse3DI	GCAATG	5 cut(s)	376, 430, 1356, 2049, 2418
Bse8I	GATNNNNATC	1 cut(s)	1647
BseAI	TCCGGA	1 cut(s)	2227
BseBI	CCWGG	9 cut(s)	226, 465, 591, 1403, 1434, 1444, 1884, 2351, 2403
BseDI	CCNNGG	9 cut(s)	225, 1387, 1442, 1443, 1794, 2161, 2173, 2377, 2461
BseGI	GGATG	9 cut(s)	309, 783, 987, 991, 1599, 1648, 2290, 2498, 2607
BseJI	GATNNNNATC	1 cut(s)	1647
BseLI	CCNNNNNNNGG	4 cut(s)	596, 1174, 2094, 2382
BseMI	GCAATG	5 cut(s)	376, 430, 1356, 2049, 2418
BseMII	CTCAG	3 cut(s)	1704, 2146, 2677
BseNI	ACTGG	7 cut(s)	565, 661, 1552, 1777, 2155, 2445, 2593
BseRI	GAGGAG	1 cut(s)	1470
BseSI	GKGCMC	2 cut(s)	1291, 2822
BseYI	CCCAGC	1 cut(s)	930
BsgI	GTGCAG	2 cut(s)	1221, 1833
Bsh1236I	CGCG	2 cut(s)	1182, 2379
BshFI	GGCC	8 cut(s)	11, 401, 717, 908, 1799, 2354, 2781, 2846
BshNI	GGYRCC	1 cut(s)	938
BsiHKAI	GWGCWC	2 cut(s)	1291, 1516
BsiHKCI	CYCGRG	1 cut(s)	607
BsiSI	CCGG	4 cut(s)	575, 1185, 1796, 2228
BsiWI	CGTACG	1 cut(s)	827
BslFI	GGGAC	4 cut(s)	50, 913, 1565, 2843
BslI	CCNNNNNNNGG	4 cut(s)	596, 1174, 2094, 2382
BsmAI	GTCTC	5 cut(s)	462, 1158, 1493, 1969, 2241
BsmFI	GGGAC	4 cut(s)	50, 913, 1565, 2843
BsnI	GGCC	8 cut(s)	11, 401, 717, 908, 1799, 2354, 2781, 2846
Bso31I	GGTCTC	1 cut(s)	1158
BsoBI	CYCGRG	1 cut(s)	607
Bsp1286I	GDGCHC	3 cut(s)	1291, 1516, 2822
Bsp13I	TCCGGA	1 cut(s)	2227
Bsp1407I	TGTACA	1 cut(s)	2604
BspANI	GGCC	8 cut(s)	11, 401, 717, 908, 1799, 2354, 2781, 2846
BspCNI	CTCAG	3 cut(s)	1705, 2145, 2676
BspEI	TCCGGA	1 cut(s)	2227
BspFNI	CGCG	2 cut(s)	1182, 2379
BspHI	TCATGA	2 cut(s)	295, 2076
BspLI	GGNNCC	7 cut(s)	402, 462, 869, 940, 1420, 1441, 1553
BspMAI	CTGCAG	1 cut(s)	244
BspMI	ACCTGC	2 cut(s)	718, 1420
BspPI	GGATC	9 cut(s)	524, 619, 862, 875, 1089, 1377, 1527, 1656, 2313
BspQI	GCTCTTC	1 cut(s)	1504
BspT107I	GGYRCC	1 cut(s)	938
BspTNI	GGTCTC	1 cut(s)	1158
BsrBI	CCGCTC	1 cut(s)	136
BsrDI	GCAATG	5 cut(s)	376, 430, 1356, 2049, 2418
BsrGI	TGTACA	1 cut(s)	2604
BsrI	ACTGG	7 cut(s)	565, 661, 1552, 1777, 2155, 2445, 2593
BssECI	CCNNGG	9 cut(s)	225, 1387, 1442, 1443, 1794, 2161, 2173, 2377, 2461
BssNI	GRCGYC	1 cut(s)	1704
BssSI	CACGAG	3 cut(s)	100, 2739, 2790
BssT1I	CCWWGG	2 cut(s)	1387, 2173
Bst2BI	CACGAG	3 cut(s)	100, 2739, 2790
Bst2UI	CCWGG	9 cut(s)	226, 465, 591, 1403, 1434, 1444, 1884, 2351, 2403
Bst4CI	ACNGT	8 cut(s)	827, 889, 1036, 1493, 1660, 2123, 2188, 2527
Bst6I	CTCTTC	3 cut(s)	1475, 1504, 1730
BstACI	GRCGYC	1 cut(s)	1704
BstAPI	GCANNNNNTGC	2 cut(s)	371, 2873
BstAUI	TGTACA	1 cut(s)	2604
BstBAI	YACGTR	1 cut(s)	1853
BstC8I	GCNNGC	6 cut(s)	367, 623, 1180, 1702, 2004, 2865
BstDEI	CTNAG	4 cut(s)	1170, 1713, 2132, 2663
BstDSI	CCRYGG	1 cut(s)	2377
BstEII	GGTNACC	2 cut(s)	577, 2168
BstF5I	GGATG	9 cut(s)	309, 783, 987, 991, 1599, 1648, 2290, 2498, 2607
BstFNI	CGCG	2 cut(s)	1182, 2379
BstH2I	RGCGCY	1 cut(s)	1860
BstHHI	GCGC	3 cut(s)	423, 1184, 1859
BstMAI	GTCTC	5 cut(s)	462, 1158, 1493, 1969, 2241
BstNI	CCWGG	9 cut(s)	226, 465, 591, 1403, 1434, 1444, 1884, 2351, 2403
BstNSI	RCATGY	4 cut(s)	621, 713, 1609, 2626
BstPI	GGTNACC	2 cut(s)	577, 2168
BstSFI	CTRYAG	1 cut(s)	240
BstSLI	GKGCMC	2 cut(s)	1291, 2822
BstUI	CGCG	2 cut(s)	1182, 2379
BstV2I	GAAGAC	3 cut(s)	509, 1451, 2002
BstX2I	RGATCY	4 cut(s)	529, 867, 1519, 1648
BstYI	RGATCY	4 cut(s)	529, 867, 1519, 1648
Bsu36I	CCTNAGG	1 cut(s)	1170
BsuI	GTATCC	1 cut(s)	1620
BsuRI	GGCC	8 cut(s)	11, 401, 717, 908, 1799, 2354, 2781, 2846
BtgI	CCRYGG	1 cut(s)	2377
BtgZI	GCGATG	3 cut(s)	609, 751, 2416
BtsCI	GGATG	9 cut(s)	309, 783, 987, 991, 1599, 1648, 2290, 2498, 2607
BtsI	GCAGTG	1 cut(s)	1003
BtsIMutI	CAGTG	7 cut(s)	1003, 1013, 1041, 1498, 1545, 2140, 2671
BveI	ACCTGC	2 cut(s)	718, 1420
Cac8I	GCNNGC	6 cut(s)	367, 623, 1180, 1702, 2004, 2865
CaiI	CAGNNNCTG	2 cut(s)	1847, 1883
CciI	TCATGA	2 cut(s)	295, 2076
CfoI	GCGC	3 cut(s)	423, 1184, 1859
Cfr13I	GGNCC	3 cut(s)	400, 1439, 1552
Cfr42I	CCGCGG	1 cut(s)	2380
CseI	GACGC	2 cut(s)	38, 1693
Csp6I	GTAC	7 cut(s)	828, 1343, 1409, 1599, 2070, 2444, 2605
CspCI	CAANNNNNGTGG	2 cut(s)	1417, 1452
CviQI	GTAC	7 cut(s)	828, 1343, 1409, 1599, 2070, 2444, 2605
DdeI	CTNAG	4 cut(s)	1170, 1713, 2132, 2663
DraI	TTTAAA	1 cut(s)	537
DraIII	CACNNNGTG	1 cut(s)	2666
EaeI	YGGCCR	4 cut(s)	9, 715, 906, 2844
Eam1104I	CTCTTC	3 cut(s)	1475, 1504, 1730
EarI	CTCTTC	3 cut(s)	1475, 1504, 1730
EciI	GGCGGA	1 cut(s)	452
Ecl136II	GAGCTC	1 cut(s)	1514
Eco130I	CCWWGG	2 cut(s)	1387, 2173
Eco147I	AGGCCT	1 cut(s)	2354
Eco24I	GRGCYC	1 cut(s)	1516
Eco31I	GGTCTC	1 cut(s)	1158
Eco32I	GATATC	2 cut(s)	496, 2205
Eco47I	GGWCC	2 cut(s)	1439, 1552
Eco47III	AGCGCT	1 cut(s)	1858
Eco53kI	GAGCTC	1 cut(s)	1514
Eco57I	CTGAAG	3 cut(s)	1602, 2921, 2951
Eco81I	CCTNAGG	1 cut(s)	1170
Eco88I	CYCGRG	1 cut(s)	607
Eco91I	GGTNACC	2 cut(s)	577, 2168
EcoICRI	GAGCTC	1 cut(s)	1514
EcoO65I	GGTNACC	2 cut(s)	577, 2168
EcoRII	CCWGG	9 cut(s)	224, 463, 589, 1401, 1432, 1442, 1882, 2349, 2401
EcoRV	GATATC	2 cut(s)	496, 2205
EcoT14I	CCWWGG	2 cut(s)	1387, 2173
EcoT38I	GRGCYC	1 cut(s)	1516
ErhI	CCWWGG	2 cut(s)	1387, 2173
FaqI	GGGAC	4 cut(s)	50, 913, 1565, 2843
FauI	CCCGC	3 cut(s)	75, 2384, 2856
FbaI	TGATCA	3 cut(s)	544, 1542, 1896
FblI	GTMKAC	1 cut(s)	2691
FokI	GGATG	9 cut(s)	296, 790, 994, 998, 1606, 1655, 2297, 2485, 2614
FriOI	GRGCYC	1 cut(s)	1516
FspBI	CTAG	3 cut(s)	1749, 1988, 2974
FspI	TGCGCA	1 cut(s)	422
GlaI	GCGC	3 cut(s)	422, 1183, 1858
GsaI	CCCAGC	1 cut(s)	934
GsuI	CTGGAG	5 cut(s)	77, 486, 582, 678, 1385
HaeII	RGCGCY	1 cut(s)	1860
HaeIII	GGCC	8 cut(s)	11, 401, 717, 908, 1799, 2354, 2781, 2846
HapII	CCGG	4 cut(s)	575, 1185, 1796, 2228
HgaI	GACGC	2 cut(s)	38, 1693
HhaI	GCGC	3 cut(s)	423, 1184, 1859
Hin1I	GRCGYC	1 cut(s)	1704
Hin6I	GCGC	3 cut(s)	421, 1182, 1857
HinP1I	GCGC	3 cut(s)	421, 1182, 1857
HincII	GTYRAC	3 cut(s)	1075, 1914, 2496
HindII	GTYRAC	3 cut(s)	1075, 1914, 2496
HindIII	AAGCTT	2 cut(s)	161, 1028
HinfI	GANTC	8 cut(s)	14, 214, 566, 808, 1188, 1300, 1835, 2915
HpaI	GTTAAC	1 cut(s)	1914
HpaII	CCGG	4 cut(s)	575, 1185, 1796, 2228
HphI	GGTGA	5 cut(s)	503, 589, 1643, 1787, 1963
Hpy188I	TCNGA	9 cut(s)	93, 121, 1193, 1508, 1864, 2310, 2465, 2567, 2901
Hpy99I	CGWCG	1 cut(s)	32
HpyCH4III	ACNGT	8 cut(s)	827, 889, 1036, 1493, 1660, 2123, 2188, 2527
HpyCH4IV	ACGT	5 cut(s)	737, 1372, 1852, 2241, 2546
HpyF3I	CTNAG	4 cut(s)	1170, 1713, 2132, 2663
HpySE526I	ACGT	5 cut(s)	737, 1372, 1852, 2241, 2546
Hsp92I	GRCGYC	1 cut(s)	1704
HspAI	GCGC	3 cut(s)	421, 1182, 1857
Kpn2I	TCCGGA	1 cut(s)	2227
Ksp22I	TGATCA	3 cut(s)	544, 1542, 1896
KspAI	GTTAAC	1 cut(s)	1914
KspI	CCGCGG	1 cut(s)	2380
LguI	GCTCTTC	1 cut(s)	1504
LmnI	GCTCC	3 cut(s)	122, 2378, 2887
MaeI	CTAG	3 cut(s)	1749, 1988, 2974
MaeII	ACGT	5 cut(s)	737, 1372, 1852, 2241, 2546
MaeIII	GTNAC	9 cut(s)	152, 276, 577, 898, 1775, 2146, 2168, 2365, 2654
MbiI	CCGCTC	1 cut(s)	136
MfeI	CAATTG	4 cut(s)	741, 783, 1146, 1329
MflI	RGATCY	4 cut(s)	529, 867, 1519, 1648
MhlI	GDGCHC	3 cut(s)	1291, 1516, 2822
MlsI	TGGCCA	2 cut(s)	717, 908
MluNI	TGGCCA	2 cut(s)	717, 908
MlyI	GAGTC	2 cut(s)	23, 2924
MmeI	TCCRAC	2 cut(s)	120, 2105
Mox20I	TGGCCA	2 cut(s)	717, 908
MroI	TCCGGA	1 cut(s)	2227
MroXI	GAANNNNTTC	3 cut(s)	570, 1299, 2470
MscI	TGGCCA	2 cut(s)	717, 908
MslI	CAYNNNNRTG	3 cut(s)	1491, 1761, 2621
Msp20I	TGGCCA	2 cut(s)	717, 908
MspA1I	CMGCKG	4 cut(s)	70, 380, 1259, 2379
MspI	CCGG	4 cut(s)	575, 1185, 1796, 2228
MunI	CAATTG	4 cut(s)	741, 783, 1146, 1329
MvaI	CCWGG	9 cut(s)	226, 465, 591, 1403, 1434, 1444, 1884, 2351, 2403
MvnI	CGCG	2 cut(s)	1182, 2379
NciI	CCSGG	1 cut(s)	1796
NlaIV	GGNNCC	7 cut(s)	402, 462, 869, 940, 1420, 1441, 1553
NmeAIII	GCCGAG	2 cut(s)	37, 1522
NmuCI	GTSAC	5 cut(s)	276, 577, 898, 1775, 2365
NsbI	TGCGCA	1 cut(s)	422
NspI	RCATGY	4 cut(s)	621, 713, 1609, 2626
OliI	CACNNNNGTG	1 cut(s)	1761
PagI	TCATGA	2 cut(s)	295, 2076
PasI	CCCWGGG	1 cut(s)	1443
PceI	AGGCCT	1 cut(s)	2354
PciI	ACATGT	2 cut(s)	709, 1605
PciSI	GCTCTTC	1 cut(s)	1504
PcsI	WCGNNNNNNNCGW	2 cut(s)	312, 1378
PdmI	GAANNNNTTC	3 cut(s)	570, 1299, 2470
PfeI	GAWTC	6 cut(s)	214, 566, 808, 1188, 1300, 1835
Pfl23II	CGTACG	1 cut(s)	827
PfoI	TCCNGGA	2 cut(s)	463, 1401
PleI	GAGTC	2 cut(s)	22, 2923
PpsI	GAGTC	2 cut(s)	22, 2923
Ppu21I	YACGTR	1 cut(s)	1853
PscI	ACATGT	2 cut(s)	709, 1605
PshBI	ATTAAT	1 cut(s)	2835
PsiI	TTATAA	1 cut(s)	2484
Psp124BI	GAGCTC	1 cut(s)	1516
Psp6I	CCWGG	9 cut(s)	224, 463, 589, 1401, 1432, 1442, 1882, 2349, 2401
PspEI	GGTNACC	2 cut(s)	577, 2168
PspFI	CCCAGC	1 cut(s)	930
PspGI	CCWGG	9 cut(s)	224, 463, 589, 1401, 1432, 1442, 1882, 2349, 2401
PspLI	CGTACG	1 cut(s)	827
PspN4I	GGNNCC	7 cut(s)	402, 462, 869, 940, 1420, 1441, 1553
PspPI	GGNCC	3 cut(s)	400, 1439, 1552
PsrI	GAACNNNNNNTAC	4 cut(s)	1362, 1394, 2692, 2724
PstI	CTGCAG	1 cut(s)	244
PstNI	CAGNNNCTG	2 cut(s)	1847, 1883
PsuI	RGATCY	4 cut(s)	529, 867, 1519, 1648
PvuII	CAGCTG	1 cut(s)	380
RsaI	GTAC	7 cut(s)	829, 1344, 1410, 1600, 2071, 2445, 2606
RsaNI	GTAC	7 cut(s)	828, 1343, 1409, 1599, 2070, 2444, 2605
RseI	CAYNNNNRTG	3 cut(s)	1491, 1761, 2621
SacI	GAGCTC	1 cut(s)	1516
SacII	CCGCGG	1 cut(s)	2380
SapI	GCTCTTC	1 cut(s)	1504
Sau96I	GGNCC	3 cut(s)	400, 1439, 1552
SchI	GAGTC	2 cut(s)	23, 2924
SduI	GDGCHC	3 cut(s)	1291, 1516, 2822
SfcI	CTRYAG	1 cut(s)	240
Sfr303I	CCGCGG	1 cut(s)	2380
SgrBI	CCGCGG	1 cut(s)	2380
SinI	GGWCC	2 cut(s)	1439, 1552
SmiMI	CAYNNNNRTG	3 cut(s)	1491, 1761, 2621
SseBI	AGGCCT	1 cut(s)	2354
SspMI	CTAG	3 cut(s)	1749, 1988, 2974
SstI	GAGCTC	1 cut(s)	1516
StuI	AGGCCT	1 cut(s)	2354
StyI	CCWWGG	2 cut(s)	1387, 2173
TaaI	ACNGT	8 cut(s)	827, 889, 1036, 1493, 1660, 2123, 2188, 2527
TaiI	ACGT	5 cut(s)	740, 1375, 1855, 2244, 2549
TaqI	TCGA	7 cut(s)	27, 696, 1119, 1154, 1473, 1967, 2022
TatI	WGTACW	1 cut(s)	2604
TauI	GCSGC	3 cut(s)	377, 1259, 1588
TfiI	GAWTC	6 cut(s)	214, 566, 808, 1188, 1300, 1835
TscAI	CASTG	7 cut(s)	1003, 1020, 1041, 1498, 1552, 2140, 2671
TseFI	GTSAC	5 cut(s)	276, 577, 898, 1775, 2365
Tsp45I	GTSAC	5 cut(s)	276, 577, 898, 1775, 2365
TspDTI	ATGAA	9 cut(s)	138, 800, 1686, 1955, 2226, 2288, 2301, 2416, 2635
TspGWI	ACGGA	4 cut(s)	1654, 1794, 2381, 2642
TspRI	CASTG	7 cut(s)	1003, 1020, 1041, 1498, 1552, 2140, 2671
VneI	GTGCAC	1 cut(s)	1287
VpaK11BI	GGWCC	2 cut(s)	1439, 1552
VspI	ATTAAT	1 cut(s)	2835
XapI	RAATTY	6 cut(s)	1197, 1448, 2018, 2234, 2552, 2642
XceI	RCATGY	4 cut(s)	621, 713, 1609, 2626
XcmI	CCANNNNNNNNNTGG	1 cut(s)	970
XmiI	GTMKAC	1 cut(s)	2691
XmnI	GAANNNNTTC	3 cut(s)	570, 1299, 2470
XspI	CTAG	3 cut(s)	1749, 1988, 2974

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.