RchiOBHm_Chr4g0412601

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002698
Physical Location & Seq
Reverse (-)
36462656 .. 36464168
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38325

Sequence Viewer

Length: 801 bp
ATGGCTGCAAGAGCTTCAAGGTTGGAAAATCAAGATGCCATTGATGCAGCAATTGTTGTAATGCTGGCTGATTCTAAGGAGGCACGAGCCGGAATTACAGAAGTTCACTTCCTTCCTTTCAATCCAACTGATAAGAGGACAGCACTTACATACCTAGACAAAGCTGGTAAAATGCAGAGTGAGCAAAGGTGCACCAGAGCAGTACCTGCTGGAACCAAAGACAGTCCTGGTGGACCTTGGGAGTTTGTTGGTCTTCTACCTTTGTTTGATCCACCACGTCACGACAGTGCTGAAACTATTAGAAGAGCTCTAGATCTTGGTGTGAGTGTTAAGATGATTATAGGCGATCAACTAGCAATTGCTAAAGAGACAGGGAGACGACTTGGGATGGGATCAAACATGTATCCATCATCTTCATTGCTTGGTGAAAATAAGAATGGATTAGATGCAAGTCTACAAATTGATGAACTCATTGAGAATGCTGATGGTTTTGCTGGTATCTTTCCAGAGCATAAATATGAGATTGTTCAAAGATTACAAATTAAGAAGCACATAGTTGGAATGACCGGTGATGGAGTTAATGATGCACCTGCCTTGAAGAAAGCAGACATAGGAATTGCTGTGGCAGATGCCACAGATGCTGCTCGTAGTGCGTCTGATATAGTACTGACACAGCCTGGATTGAGTGTTATTATTAGTGCTGTTCTGACAAGCCGTGCAATCTTTCAAAGAATGAAGAACTACACAGTAAGGGGATTCTCCCTTCTCATTTTTTTAGGAATTGGACCAGCCTGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

266

Amino Acids

28.61

Weight (kDa)

6.44

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 81 - 203 6.4e-10 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 598
Acc36I ACCTGC 2 cut(s) 214, 598
AccI GTMKAC 1 cut(s) 454
AclWI GGATC 2 cut(s) 263, 400
AfaI GTAC 2 cut(s) 204, 666
AflIII ACRYGT 1 cut(s) 399
AgeI ACCGGT 1 cut(s) 566
AgsI TTSAA 5 cut(s) 18, 121, 530, 598, 728
AjiI CACGTC 1 cut(s) 278
AjnI CCWGG 3 cut(s) 226, 676, 791
AleI CACNNNNGTG 1 cut(s) 285
AluBI AGCT 3 cut(s) 14, 164, 308
AluI AGCT 3 cut(s) 14, 164, 308
Alw21I GWGCWC 2 cut(s) 194, 310
Alw26I GTCTC 2 cut(s) 362, 370
Alw44I GTGCAC 1 cut(s) 190
AlwI GGATC 2 cut(s) 263, 400
AlwNI CAGNNNCTG 2 cut(s) 206, 641
ApaLI GTGCAC 1 cut(s) 190
ApeKI GCWGC 3 cut(s) 5, 47, 641
AsiGI ACCGGT 1 cut(s) 566
AspS9I GGNCC 2 cut(s) 233, 785
AsuHPI GGTGA 2 cut(s) 437, 581
AvaII GGWCC 2 cut(s) 233, 785
BaeGI GKGCMC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 310
BauI CACGAG 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 245
Bbv12I GWGCWC 2 cut(s) 194, 310
BbvI GCAGC 2 cut(s) 59, 628
BccI CCATC 4 cut(s) 382, 415, 479, 566
BceAI ACGGC 1 cut(s) 699
BciT130I CCWGG 3 cut(s) 228, 678, 793
BciVI GTATCC 1 cut(s) 414
BcoDI GTCTC 2 cut(s) 362, 370
BfaI CTAG 3 cut(s) 155, 311, 353
BfuAI ACCTGC 2 cut(s) 214, 598
BfuI GTATCC 1 cut(s) 414
BglII AGATCT 1 cut(s) 313
BisI GCNGC 3 cut(s) 6, 48, 642
BlsI GCNGC 3 cut(s) 7, 49, 643
BmcAI AGTACT 1 cut(s) 666
Bme1390I CCNGG 3 cut(s) 228, 678, 793
Bme18I GGWCC 2 cut(s) 233, 785
BmgBI CACGTC 1 cut(s) 278
BmgT120I GGNCC 2 cut(s) 233, 785
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 3 cut(s) 228, 678, 793
BmsI GCATC 6 cut(s) 25, 34, 436, 574, 619, 628
BpiI GAAGAC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 236
BsaWI WCCGGW 1 cut(s) 566
Bse118I RCCGGY 1 cut(s) 566
Bse3DI GCAATG 1 cut(s) 416
BseBI CCWGG 3 cut(s) 228, 678, 793
BseDI CCNNGG 1 cut(s) 236
BseGI GGATG 1 cut(s) 393
BseMI GCAATG 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 2 cut(s) 59, 628
BshTI ACCGGT 1 cut(s) 566
BsiHKAI GWGCWC 2 cut(s) 194, 310
BsiSI CCGG 2 cut(s) 90, 567
BsmAI GTCTC 2 cut(s) 362, 370
BsmBI CGTCTC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 484
Bsp1286I GDGCHC 2 cut(s) 194, 310
Bsp143I GATC 4 cut(s) 268, 313, 346, 392
BspLI GGNNCC 1 cut(s) 214
BspMI ACCTGC 2 cut(s) 214, 598
BspPI GGATC 2 cut(s) 263, 400
BspQI GCTCTTC 1 cut(s) 298
BsrDI GCAATG 1 cut(s) 416
BsrFI RCCGGY 1 cut(s) 566
BssAI RCCGGY 1 cut(s) 566
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 4 cut(s) 268, 313, 346, 392
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 236
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 3 cut(s) 228, 678, 793
Bst4CI ACNGT 3 cut(s) 224, 287, 748
Bst6I CTCTTC 1 cut(s) 298
BstAPI GCANNNNNTGC 1 cut(s) 206
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 1 cut(s) 75
BstF5I GGATG 1 cut(s) 393
BstKTI GATC 4 cut(s) 271, 316, 349, 395
BstMAI GTCTC 2 cut(s) 362, 370
BstMBI GATC 4 cut(s) 268, 313, 346, 392
BstMWI GCNNNNNNNGC 6 cut(s) 11, 44, 181, 206, 638, 650
BstNI CCWGG 3 cut(s) 228, 678, 793
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 3 cut(s) 226, 676, 791
BstSLI GKGCMC 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 59, 628
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BsuI GTATCC 1 cut(s) 414
BtrI CACGTC 1 cut(s) 278
BtsCI GGATG 1 cut(s) 393
BtsIMutI CAGTG 1 cut(s) 292
BveI ACCTGC 2 cut(s) 214, 598
Cac8I GCNNGC 1 cut(s) 66
CaiI CAGNNNCTG 2 cut(s) 206, 641
Cfr10I RCCGGY 1 cut(s) 566
Cfr13I GGNCC 2 cut(s) 233, 785
CseI GACGC 1 cut(s) 642
Csp6I GTAC 2 cut(s) 203, 665
CspAI ACCGGT 1 cut(s) 566
CviAII CATG 1 cut(s) 400
CviJI RGCY 9 cut(s) 5, 14, 68, 89, 164, 308, 676, 714, 791
CviKI_1 RGCY 9 cut(s) 5, 14, 68, 89, 164, 308, 676, 714, 791
CviQI GTAC 2 cut(s) 203, 665
DdeI CTNAG 1 cut(s) 75
DpnI GATC 4 cut(s) 270, 315, 348, 394
DpnII GATC 4 cut(s) 268, 313, 346, 392
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
Ecl136II GAGCTC 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 310
Eco47I GGWCC 2 cut(s) 233, 785
Eco53kI GAGCTC 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 308
EcoRII CCWGG 3 cut(s) 226, 676, 791
EcoT14I CCWWGG 1 cut(s) 236
EcoT38I GRGCYC 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 236
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 1 cut(s) 403
FaiI YATR 8 cut(s) 151, 341, 401, 513, 519, 554, 611, 662
FatI CATG 1 cut(s) 399
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 3 cut(s) 6, 48, 642
FokI GGATG 1 cut(s) 400
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 3 cut(s) 6, 48, 642
FspBI CTAG 3 cut(s) 155, 311, 353
GluI GCNGC 3 cut(s) 6, 48, 642
HapII CCGG 2 cut(s) 90, 567
HgaI GACGC 1 cut(s) 642
Hin1II CATG 1 cut(s) 403
HinfI GANTC 2 cut(s) 71, 756
HpaII CCGG 2 cut(s) 90, 567
HphI GGTGA 2 cut(s) 437, 581
Hpy166II GTNNAC 4 cut(s) 106, 192, 233, 455
Hpy188I TCNGA 2 cut(s) 658, 708
Hpy188III TCNNGA 4 cut(s) 32, 281, 311, 506
Hpy8I GTNNAC 4 cut(s) 106, 192, 233, 455
HpyAV CCTTC 2 cut(s) 122, 773
HpyCH4III ACNGT 3 cut(s) 224, 287, 748
HpyCH4IV ACGT 1 cut(s) 277
HpyCH4V TGCA 7 cut(s) 8, 47, 175, 192, 449, 587, 719
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 44, 181, 206, 638, 650
HpyF3I CTNAG 1 cut(s) 75
HpySE526I ACGT 1 cut(s) 277
Hsp92II CATG 1 cut(s) 403
Kzo9I GATC 4 cut(s) 268, 313, 346, 392
LguI GCTCTTC 1 cut(s) 298
Lsp1109I GCAGC 2 cut(s) 59, 628
LweI GCATC 6 cut(s) 25, 34, 436, 574, 619, 628
MaeI CTAG 3 cut(s) 155, 311, 353
MaeII ACGT 1 cut(s) 277
MaeIII GTNAC 1 cut(s) 278
MalI GATC 4 cut(s) 270, 315, 348, 394
MboI GATC 4 cut(s) 268, 313, 346, 392
MboII GAAGA 5 cut(s) 245, 315, 405, 610, 748
MfeI CAATTG 2 cut(s) 51, 357
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 194, 310
MluCI AATT 7 cut(s) 51, 93, 357, 459, 540, 615, 780
MmeI TCCRAC 2 cut(s) 149, 538
MnlI CCTC 2 cut(s) 73, 129
MseI TTAA 3 cut(s) 330, 543, 579
MslI CAYNNNNRTG 2 cut(s) 285, 516
MspI CCGG 2 cut(s) 90, 567
MspR9I CCNGG 3 cut(s) 228, 678, 793
MunI CAATTG 2 cut(s) 51, 357
Mva1269I GAATGC 1 cut(s) 484
MvaI CCWGG 3 cut(s) 228, 678, 793
MwoI GCNNNNNNNGC 6 cut(s) 11, 44, 181, 206, 638, 650
NdeII GATC 4 cut(s) 268, 313, 346, 392
NlaIII CATG 1 cut(s) 403
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 278
NspI RCATGY 1 cut(s) 403
OliI CACNNNNGTG 1 cut(s) 285
PaqCI CACCTGC 1 cut(s) 598
PciI ACATGT 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 298
PctI GAATGC 1 cut(s) 484
PfeI GAWTC 2 cut(s) 71, 756
PinAI ACCGGT 1 cut(s) 566
PkrI GCNGC 3 cut(s) 7, 49, 643
PscI ACATGT 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 310
Psp6I CCWGG 3 cut(s) 226, 676, 791
PspGI CCWGG 3 cut(s) 226, 676, 791
PspN4I GGNNCC 1 cut(s) 214
PspPI GGNCC 2 cut(s) 233, 785
PstNI CAGNNNCTG 2 cut(s) 206, 641
PsuI RGATCY 1 cut(s) 313
RsaI GTAC 2 cut(s) 204, 666
RsaNI GTAC 2 cut(s) 203, 665
RseI CAYNNNNRTG 2 cut(s) 285, 516
SacI GAGCTC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 298
SaqAI TTAA 3 cut(s) 330, 543, 579
SatI GCNGC 3 cut(s) 6, 48, 642
Sau3AI GATC 4 cut(s) 268, 313, 346, 392
Sau96I GGNCC 2 cut(s) 233, 785
ScaI AGTACT 1 cut(s) 666
ScrFI CCNGG 3 cut(s) 228, 678, 793
SduI GDGCHC 2 cut(s) 194, 310
SfaNI GCATC 6 cut(s) 25, 34, 436, 574, 619, 628
SinI GGWCC 2 cut(s) 233, 785
SmiMI CAYNNNNRTG 2 cut(s) 285, 516
Sse9I AATT 7 cut(s) 51, 93, 357, 459, 540, 615, 780
SspMI CTAG 3 cut(s) 155, 311, 353
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 3 cut(s) 226, 676, 791
StyI CCWWGG 1 cut(s) 236
TaaI ACNGT 3 cut(s) 224, 287, 748
TaiI ACGT 1 cut(s) 280
TasI AATT 7 cut(s) 51, 93, 357, 459, 540, 615, 780
TatI WGTACW 1 cut(s) 664
TfiI GAWTC 2 cut(s) 71, 756
Tru1I TTAA 3 cut(s) 330, 543, 579
Tru9I TTAA 3 cut(s) 330, 543, 579
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 3 cut(s) 5, 47, 641
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 3 cut(s) 405, 480, 749
TspRI CASTG 1 cut(s) 292
VneI GTGCAC 1 cut(s) 190
VpaK11BI GGWCC 2 cut(s) 233, 785
XbaI TCTAGA 1 cut(s) 310
XceI RCATGY 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 454
XspI CTAG 3 cut(s) 155, 311, 353
ZrmI AGTACT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.