MD04G1058300.v1.1

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002698
Physical Location & Seq
Forward (+)
7595890 .. 7601504
5615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1058300.v1.1.491

Sequence Viewer

Length: 2922 bp
ATGGATGCCCTTGAGACTATTTTGAAGGAAGCTGTTGATCTGGAGAACATACCCGTTGAGGAAGTCTTCGAAAATTTGAAATGTACAGCTGAGGGTCTCAGTACTGACGAGGTACAAAAGCGGTTGGAGATGTTTGGCTACAACAAACTTGAAGAGAAAAAGGAGAGCAAACTACTCAAATTTCTGGGATTTATGTGGAACCCTTTGTCTTGGGTTATGGAAGCAGCTGCGTTAATGTCCATATGTCTTGCGCACGGAGGCGGACTGGGTATGGATTATCACGATTTCGTGGGTATCATTATATTACTCATAGTTAATTCAACTATAAGTTTTATAGAGGAAAACAATGCTGGTAATGCAGCTCAAGCCCTTATGGCAAGACTGGCTCCAAAGGCGAAGGTTTTACGAGATGGAAAATGGAGTGAGGAAGATGCTTCTGTGCTGGTTCCTGGAGACATTATCAGTATCAAATTGGGTGATATTATTCCTGCTGATGCTCGTTTGCTCGAAGGAGATGCTTTAAAAATTGATCAGTCCGCTCTTACAGGAGAGTCACTTCCAGTAACTAAGAATGCCGGTGATGGTGTATACTCTGGTTCAACATGTAAACAAGGTGAACTTGAAGCGGTTGTCATTGCAACTGGAGTACATGCCTTCTTCGGAAAAGCTGCCCATCTTGTTGAAAACACAACTCATGTTGGGCACTTTCAGCAGGTCTTAACATCTATTGGAAACTTTTGCATTTGTTCAATAGCCACTGGACTTGTGATTGAGCTCATTGTCTTCATTTGTCAAAAAAGAGGTTATCGTCCTTCGGTAGACAACCTTCTTGTTCTACTTATCGGTGGCATCCCAATTGCTATGCCAACAGTTCTGTCTGTCACCATGGCCATTGGTTCTCATCGGTTGGCTCAGCAGGGTGCAATTACCAAGAGAATGACAGCTATTGAAGAAATGGCTGGGATGGATGTGCTCTGCAGTGATAAAACTGGAACTTTAACTCTCAACAAACTAACAGTGGACAAGAATTTGATAGAGGTGATAATCTTGATCCTGTCGGCTTCAAGATTGGAAAATCAAGATGCTATTGATGCAGCAATTGTTGCCATGCTAGCTGATCCTAAGGAGGCTCGCGCGGGGATTACAGAAGTTCACTTCCTTCCATTCAATCCAACTGATAAGAGGACAGCACTTACATACATAGACACAACCGGTAACATGCACAGAGTGAGCAAAGGTGCACCAGAGCAGATACTCAATTTGGCAAGCAACAAATCAGAAATTGAAAAAAGGGTACATACAGTAATTGAAAAATTCGCAGAGCGTGGACTTCGATCCCTTGCTGTTGCACGGCAGGAAGTTCCTGCTGGTACGAAAGACAGTCCCGGTGGACCTTGGGAGTTTGTCGGTCTTCTCCCCCTATTTGATCCACCACGTCATGACAGCGCTGAAACTATTAGAAGAGCTCTAGATTTGGGTGTGAGTGTTAAAATGATCACGGGTGATCAACTGGCAATTGGAAAAGAAACTGGGAGACGACTTGGGATGGGCACAAACATGTATCCATCATCGGCGTTGCTTGGTGAAAATAAGAATAGCATAGATGGAACTCTAGTAATTGATGAACTCATTGAGAATGCTGATGGTTTTGCTGGTGTATTTCCTGAACATAAATATGAGATCGTTCAGCGATTACAAGGTAAGAAGCACATAGTCGGAATGACAGGTGATGGAGTCAATGATGCACCAGCATTGAAGAAAGCAGACATAGGAATTGCTGTCGCCGATGCCACAGATGCAGCCCGTGGTGCATCTGATATAGTACTGACAGAACCTGGTTTGAGTGTGATTATCAGTGCTGTTTTGACAAGCCGTGCGATCTTTCAAAGAATGAAGAATTACACAATATACGCAGTATCTATAACAATACGTATTGTGCTCGGTTTCTTGTTGCTTGCGGTATTCTGGAAATTTGATTTCCCTCCTTTTATGGTCCTAATCATTGCCATTCTTAATGATGGTACTATTATGACGATATCCAAGGACAGGGTTAAGCCATCTCCAGTTCCTGACAGTTGGAAGCTGAGTGAAATCTTTGCAACCGGGATTGTGTTAGGCAGTTATCTTGCCGTTACCACAGTTATATTCTTCTCGATCATTTACGATACTAAATTTTTTCCGATGAAATTGTTCTCACATTTTGTACTCGATTACAATGATCAAGTTTTCACCTTCTCTCGTTCTCTCTCGCAGAAAAAATTCGGTGTAGAAGATATCTTCAATGAGAATCTCTATTATACCAACGGGACCCTCAATCCGGGAATGAATGCCAAGCTATCATCTGCTCTGTACCTTCAAGTTAGCACCATCAGCCAGGCTCTAATTTTTGTCACTCGCTCCCGAGGATGGTCATTCCTGGAAGTACCTGGTCTGCTTCTGCTTAGTGCTTTCGTAATTGCTCAACTGATTGCTACATTAATATCTGCTTTAGTAACATGGAAAATTGCTAGAATTCAAGCAATCGGCTGGAAATGGTGTGGTGTCGTATGGATATACAACATTCTAATCTACATGCTACTTGATCCTGTCAAGATTTTCGTTCGATATGCACTCAGCGGGAGGGCTTGGAGTTTGGTATTGAACAAAAGAACGGCTTTCAACACCCAAAAGGACTTCGGTAAAGAGTTCCGTGAGGCTGCATGGGCGGCAGAACAGCGGACGGTCCATGGCCTCCCATCTATGGAGGCAAAGAACATTCCTGAGAGGCACACATTCAGGGATGTTAGCATCATGGCTGAAGAAGCCAGGAGACGTGCAGAGATTGCAAGACTAAGGGAGCTTCACACTCTGAAGGGGAAGGTCGAGTCCTTTGCAAAGCTAAGGGGTTTAGACATCGAAACGAACCCAAATTACACTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

974

Amino Acids

106.86

Weight (kDa)

6.25

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 16 - 80 1.2e-15 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 128 - 228 5.6e-31 P-type ATPase actuator domain
Hydrolase PF00702 322 - 589 1.6e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 360 - 480 1e-05 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 252
Acc36I ACCTGC 1 cut(s) 703
AccBSI CCGCTC 1 cut(s) 539
AccI GTMKAC 2 cut(s) 588, 819
AccII CGCG 2 cut(s) 1134, 1136
AciI CCGC 9 cut(s) 121, 261, 537, 626, 1136, 1958, 2616, 2705, 2716
AclWI GGATC 5 cut(s) 1045, 1112, 1329, 1421, 2576
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 8 cut(s) 73, 179, 1027, 1313, 1970, 2171, 2258, 2511
AcuI CTGAAG 2 cut(s) 2817, 2870
AdeI CACNNNGTG 1 cut(s) 1228
AfeI AGCGCT 1 cut(s) 1447
AfiI CCNNNNNNNGG 4 cut(s) 2046, 2317, 2406, 2740
AflIII ACRYGT 2 cut(s) 602, 1557
AgeI ACCGGT 1 cut(s) 1211
AjiI CACGTC 2 cut(s) 1436, 2813
AjnI CCWGG 6 cut(s) 448, 1834, 2373, 2415, 2425, 2804
Alw21I GWGCWC 5 cut(s) 777, 975, 1243, 1468, 1941
Alw26I GTCTC 5 cut(s) 8, 101, 447, 1528, 2803
Alw44I GTGCAC 1 cut(s) 1239
AlwI GGATC 5 cut(s) 1045, 1112, 1329, 1421, 2576
AlwNI CAGNNNCTG 2 cut(s) 1835, 2069
Ama87I CYCGRG 1 cut(s) 2400
Aor51HI AGCGCT 1 cut(s) 1447
AoxI GGCC 2 cut(s) 888, 2728
ApaLI GTGCAC 1 cut(s) 1239
ApeKI GCWGC 7 cut(s) 224, 227, 359, 668, 1094, 1799, 2696
ApoI RAATTY 8 cut(s) 73, 179, 1027, 1313, 1970, 2171, 2258, 2511
AseI ATTAAT 1 cut(s) 2477
AsiGI ACCGGT 1 cut(s) 1211
Asp700I GAANNNNTTC 2 cut(s) 2189, 2258
AspLEI GCGC 3 cut(s) 253, 1136, 1448
AspS9I GGNCC 4 cut(s) 1391, 1993, 2307, 2722
AsuC2I CCSGG 3 cut(s) 1386, 2104, 2319
AsuHPI GGTGA 9 cut(s) 488, 590, 626, 874, 1051, 1514, 1595, 1739, 2221
AsuII TTCGAA 1 cut(s) 69
AsuNHI GCTAGC 1 cut(s) 1111
AvaI CYCGRG 1 cut(s) 2400
AvaII GGWCC 4 cut(s) 1391, 1993, 2307, 2722
AxyI CCTNAGG 1 cut(s) 1122
BaeGI GKGCMC 3 cut(s) 705, 1243, 1553
BalI TGGCCA 1 cut(s) 890
BanII GRGCYC 2 cut(s) 777, 1468
BarI GAAGNNNNNNTAC 2 cut(s) 810, 842
BbsI GAAGAC 3 cut(s) 58, 775, 1403
Bbv12I GWGCWC 5 cut(s) 777, 975, 1243, 1468, 1941
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 7 cut(s) 214, 236, 371, 655, 1106, 1811, 2683
BceAI ACGGC 4 cut(s) 1367, 1857, 2114, 2667
BcgI CGANNNNNNTGC 4 cut(s) 497, 531, 2852, 2886
BciT130I CCWGG 6 cut(s) 450, 1836, 2375, 2417, 2427, 2806
BciVI GTATCC 1 cut(s) 1572
BclI TGATCA 4 cut(s) 529, 1494, 1504, 2218
BcnI CCSGG 3 cut(s) 1386, 2104, 2319
BcoDI GTCTC 5 cut(s) 8, 101, 447, 1528, 2803
BfaI CTAG 4 cut(s) 1112, 1469, 1613, 2508
BfmI CTRYAG 1 cut(s) 976
BfoI RGCGCY 1 cut(s) 1449
BfuAI ACCTGC 1 cut(s) 703
BfuI GTATCC 1 cut(s) 1572
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 8 cut(s) 225, 228, 360, 669, 1095, 1800, 2697, 2706
BlpI GCTNAGC 1 cut(s) 912
BlsI GCNGC 8 cut(s) 226, 229, 361, 670, 1096, 1801, 2698, 2707
BmcAI AGTACT 2 cut(s) 103, 1824
Bme1390I CCNGG 9 cut(s) 450, 1386, 1836, 2104, 2319, 2375, 2417, 2427, 2806
Bme18I GGWCC 4 cut(s) 1391, 1993, 2307, 2722
BmeT110I CYCGRG 1 cut(s) 2400
BmgBI CACGTC 2 cut(s) 1436, 2813
BmgT120I GGNCC 4 cut(s) 1391, 1993, 2307, 2722
BmiI GGNNCC 5 cut(s) 200, 387, 447, 2308, 2309
BmrFI CCNGG 9 cut(s) 450, 1386, 1836, 2104, 2319, 2375, 2417, 2427, 2806
BmrI ACTGGG 2 cut(s) 275, 1539
BmtI GCTAGC 1 cut(s) 1115
BmuI ACTGGG 2 cut(s) 275, 1539
BpiI GAAGAC 3 cut(s) 58, 775, 1403
BpmI CTGGAG 4 cut(s) 62, 471, 663, 2046
Bpu10I CCTNAGC 2 cut(s) 90, 2879
Bpu1102I GCTNAGC 1 cut(s) 912
Bpu14I TTCGAA 1 cut(s) 69
BpuEI CTTGAG 2 cut(s) 32, 348
BpuMI CCSGG 3 cut(s) 1386, 2104, 2319
BsaAI YACGTR 1 cut(s) 1931
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 6 cut(s) 885, 1394, 1804, 2040, 2401, 2725
BsaWI WCCGGW 1 cut(s) 1211
Bsc4I CCNNNNNNNGG 4 cut(s) 2046, 2317, 2406, 2740
Bse118I RCCGGY 2 cut(s) 575, 1211
Bse1I ACTGG 9 cut(s) 270, 387, 560, 646, 763, 994, 1515, 1534, 2063
Bse21I CCTNAGG 1 cut(s) 1122
Bse3DI GCAATG 2 cut(s) 633, 2001
BseBI CCWGG 6 cut(s) 450, 1836, 2375, 2417, 2427, 2806
BseDI CCNNGG 6 cut(s) 885, 1394, 1804, 2040, 2401, 2725
BseGI GGATG 7 cut(s) 10, 849, 969, 973, 1551, 2411, 2785
BseLI CCNNNNNNNGG 4 cut(s) 2046, 2317, 2406, 2740
BseMI GCAATG 2 cut(s) 633, 2001
BseMII CTCAG 6 cut(s) 81, 112, 926, 2075, 2626, 2751
BseNI ACTGG 9 cut(s) 270, 387, 560, 646, 763, 994, 1515, 1534, 2063
BseSI GKGCMC 3 cut(s) 705, 1243, 1553
BseXI GCAGC 7 cut(s) 214, 236, 371, 655, 1106, 1811, 2683
BseYI CCCAGC 1 cut(s) 959
BsgI GTGCAG 1 cut(s) 2835
Bsh1236I CGCG 2 cut(s) 1134, 1136
BshFI GGCC 2 cut(s) 890, 2730
BshTI ACCGGT 1 cut(s) 1211
BsiHKAI GWGCWC 5 cut(s) 777, 975, 1243, 1468, 1941
BsiHKCI CYCGRG 1 cut(s) 2400
BsiSI CCGG 5 cut(s) 576, 1212, 1386, 2103, 2318
BslFI GGGAC 2 cut(s) 1368, 2320
BslI CCNNNNNNNGG 4 cut(s) 2046, 2317, 2406, 2740
BsmAI GTCTC 5 cut(s) 8, 101, 447, 1528, 2803
BsmBI CGTCTC 2 cut(s) 1528, 2803
BsmFI GGGAC 2 cut(s) 1368, 2320
BsmI GAATGC 3 cut(s) 577, 1642, 2332
BsnI GGCC 2 cut(s) 890, 2730
Bso31I GGTCTC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 2400
Bsp119I TTCGAA 1 cut(s) 69
Bsp1286I GDGCHC 7 cut(s) 705, 777, 975, 1243, 1468, 1553, 1941
Bsp1407I TGTACA 1 cut(s) 83
Bsp1720I GCTNAGC 1 cut(s) 912
Bsp19I CCATGG 2 cut(s) 885, 2725
BspACI CCGC 9 cut(s) 121, 261, 537, 626, 1136, 1958, 2616, 2705, 2716
BspANI GGCC 2 cut(s) 890, 2730
BspCNI CTCAG 6 cut(s) 82, 111, 925, 2076, 2625, 2752
BspFNI CGCG 2 cut(s) 1134, 1136
BspHI TCATGA 1 cut(s) 1438
BspLI GGNNCC 5 cut(s) 200, 387, 447, 2308, 2309
BspMAI CTGCAG 1 cut(s) 980
BspMI ACCTGC 1 cut(s) 703
BspOI GCTAGC 1 cut(s) 1115
BspPI GGATC 5 cut(s) 1045, 1112, 1329, 1421, 2576
BspQI GCTCTTC 1 cut(s) 1456
BspT104I TTCGAA 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 539
BsrDI GCAATG 2 cut(s) 633, 2001
BsrFI RCCGGY 2 cut(s) 575, 1211
BsrGI TGTACA 1 cut(s) 83
BsrI ACTGG 9 cut(s) 270, 387, 560, 646, 763, 994, 1515, 1534, 2063
BssAI RCCGGY 2 cut(s) 575, 1211
BssECI CCNNGG 6 cut(s) 885, 1394, 1804, 2040, 2401, 2725
BssNAI GTATAC 1 cut(s) 589
BssT1I CCWWGG 4 cut(s) 885, 1394, 2040, 2725
Bst1107I GTATAC 1 cut(s) 589
Bst2UI CCWGG 6 cut(s) 450, 1836, 2375, 2417, 2427, 2806
Bst4CI ACNGT 7 cut(s) 871, 1018, 1303, 1382, 2075, 2140, 2722
Bst6I CTCTTC 2 cut(s) 147, 1456
BstAPI GCANNNNNTGC 2 cut(s) 1103, 2822
BstAUI TGTACA 1 cut(s) 83
BstBAI YACGTR 1 cut(s) 1931
BstBI TTCGAA 1 cut(s) 69
BstC8I GCNNGC 4 cut(s) 1113, 1132, 1267, 1956
BstDSI CCRYGG 3 cut(s) 885, 1804, 2725
BstF5I GGATG 7 cut(s) 10, 849, 969, 973, 1551, 2411, 2785
BstFNI CGCG 2 cut(s) 1134, 1136
BstH2I RGCGCY 1 cut(s) 1449
BstHHI GCGC 3 cut(s) 253, 1136, 1448
BstMAI GTCTC 5 cut(s) 8, 101, 447, 1528, 2803
BstNI CCWGG 6 cut(s) 450, 1836, 2375, 2417, 2427, 2806
BstNSI RCATGY 5 cut(s) 606, 653, 1222, 1561, 2575
BstSCI CCNGG 9 cut(s) 448, 1384, 1834, 2102, 2317, 2373, 2415, 2425, 2804
BstSFI CTRYAG 1 cut(s) 976
BstSLI GKGCMC 3 cut(s) 705, 1243, 1553
BstSNI TACGTA 1 cut(s) 1931
BstUI CGCG 2 cut(s) 1134, 1136
BstV1I GCAGC 7 cut(s) 214, 236, 371, 655, 1106, 1811, 2683
BstV2I GAAGAC 3 cut(s) 58, 775, 1403
BstZ17I GTATAC 1 cut(s) 589
Bsu36I CCTNAGG 1 cut(s) 1122
BsuI GTATCC 1 cut(s) 1572
BsuRI GGCC 2 cut(s) 890, 2730
BtgI CCRYGG 3 cut(s) 885, 1804, 2725
BtrI CACGTC 2 cut(s) 1436, 2813
BtsCI GGATG 7 cut(s) 10, 849, 969, 973, 1551, 2411, 2785
BtsI GCAGTG 1 cut(s) 985
BtsIMutI CAGTG 4 cut(s) 756, 985, 1023, 1861
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 4 cut(s) 1113, 1132, 1267, 1956
CaiI CAGNNNCTG 2 cut(s) 1835, 2069
CciI TCATGA 1 cut(s) 1438
CfoI GCGC 3 cut(s) 253, 1136, 1448
Cfr10I RCCGGY 2 cut(s) 575, 1211
Cfr13I GGNCC 4 cut(s) 1391, 1993, 2307, 2722
CsiI ACCWGGT 2 cut(s) 1834, 2425
CspAI ACCGGT 1 cut(s) 1211
CspCI CAANNNNNGTGG 2 cut(s) 745, 780
DraI TTTAAA 1 cut(s) 522
DraIII CACNNNGTG 1 cut(s) 1228
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 2 cut(s) 147, 1456
EarI CTCTTC 2 cut(s) 147, 1456
EciI GGCGGA 1 cut(s) 276
Ecl136II GAGCTC 2 cut(s) 775, 1466
Eco105I TACGTA 1 cut(s) 1931
Eco130I CCWWGG 4 cut(s) 885, 1394, 2040, 2725
Eco24I GRGCYC 2 cut(s) 777, 1468
Eco31I GGTCTC 1 cut(s) 101
Eco32I GATATC 2 cut(s) 2037, 2275
Eco47I GGWCC 4 cut(s) 1391, 1993, 2307, 2722
Eco47III AGCGCT 1 cut(s) 1447
Eco53kI GAGCTC 2 cut(s) 775, 1466
Eco57I CTGAAG 2 cut(s) 2817, 2870
Eco81I CCTNAGG 1 cut(s) 1122
Eco88I CYCGRG 1 cut(s) 2400
EcoICRI GAGCTC 2 cut(s) 775, 1466
EcoO109I RGGNCCY 1 cut(s) 2307
EcoRI GAATTC 1 cut(s) 2511
EcoRII CCWGG 6 cut(s) 448, 1834, 2373, 2415, 2425, 2804
EcoRV GATATC 2 cut(s) 2037, 2275
EcoT14I CCWWGG 4 cut(s) 885, 1394, 2040, 2725
EcoT38I GRGCYC 2 cut(s) 777, 1468
ErhI CCWWGG 4 cut(s) 885, 1394, 2040, 2725
Esp3I CGTCTC 2 cut(s) 1528, 2803
FalI AAGNNNNNCTT 4 cut(s) 603, 635, 2638, 2670
FaqI GGGAC 2 cut(s) 1368, 2320
FauI CCCGC 2 cut(s) 1129, 2609
FauNDI CATATG 1 cut(s) 242
FbaI TGATCA 4 cut(s) 529, 1494, 1504, 2218
FblI GTMKAC 2 cut(s) 588, 819
Fnu4HI GCNGC 8 cut(s) 225, 228, 360, 669, 1095, 1800, 2697, 2706
FokI GGATG 7 cut(s) 17, 836, 976, 980, 1558, 2418, 2792
FriOI GRGCYC 2 cut(s) 777, 1468
Fsp4HI GCNGC 8 cut(s) 225, 228, 360, 669, 1095, 1800, 2697, 2706
FspBI CTAG 4 cut(s) 1112, 1469, 1613, 2508
FspI TGCGCA 1 cut(s) 252
GlaI GCGC 3 cut(s) 252, 1135, 1447
GluI GCNGC 8 cut(s) 225, 228, 360, 669, 1095, 1800, 2697, 2706
GsaI CCCAGC 1 cut(s) 963
GsuI CTGGAG 4 cut(s) 62, 471, 663, 2046
HaeII RGCGCY 1 cut(s) 1449
HaeIII GGCC 2 cut(s) 890, 2730
HapII CCGG 5 cut(s) 576, 1212, 1386, 2103, 2318
HhaI GCGC 3 cut(s) 253, 1136, 1448
Hin6I GCGC 3 cut(s) 251, 1134, 1446
HinP1I GCGC 3 cut(s) 251, 1134, 1446
HinfI GANTC 4 cut(s) 551, 1734, 2287, 2864
HpaII CCGG 5 cut(s) 576, 1212, 1386, 2103, 2318
HphI GGTGA 9 cut(s) 488, 590, 626, 874, 1051, 1514, 1595, 1739, 2221
Hpy166II GTNNAC 9 cut(s) 589, 608, 617, 820, 1021, 1153, 1241, 1328, 1391
Hpy188I TCNGA 6 cut(s) 662, 1279, 1718, 1816, 2181, 2850
Hpy8I GTNNAC 9 cut(s) 589, 608, 617, 820, 1021, 1153, 1241, 1328, 1391
HpyCH4III ACNGT 7 cut(s) 871, 1018, 1303, 1382, 2075, 2140, 2722
HpyCH4IV ACGT 3 cut(s) 1435, 1930, 2812
HpySE526I ACGT 3 cut(s) 1435, 1930, 2812
HspAI GCGC 3 cut(s) 251, 1134, 1446
KflI GGGWCCC 1 cut(s) 2307
Ksp22I TGATCA 4 cut(s) 529, 1494, 1504, 2218
LguI GCTCTTC 1 cut(s) 1456
LmnI GCTCC 3 cut(s) 391, 2402, 2836
Lsp1109I GCAGC 7 cut(s) 214, 236, 371, 655, 1106, 1811, 2683
MabI ACCWGGT 2 cut(s) 1834, 2425
MaeI CTAG 4 cut(s) 1112, 1469, 1613, 2508
MaeII ACGT 3 cut(s) 1435, 1930, 2812
MaeIII GTNAC 7 cut(s) 552, 562, 880, 1214, 2131, 2389, 2491
MbiI CCGCTC 1 cut(s) 539
MfeI CAATTG 3 cut(s) 855, 1098, 1515
MhlI GDGCHC 7 cut(s) 705, 777, 975, 1243, 1468, 1553, 1941
MlsI TGGCCA 1 cut(s) 890
MluNI TGGCCA 1 cut(s) 890
MlyI GAGTC 3 cut(s) 560, 1743, 2873
MmeI TCCRAC 4 cut(s) 105, 1196, 1696, 2057
Mox20I TGGCCA 1 cut(s) 890
MroXI GAANNNNTTC 2 cut(s) 2189, 2258
MscI TGGCCA 1 cut(s) 890
MseI TTAA 9 cut(s) 233, 315, 521, 719, 998, 1488, 2013, 2052, 2477
MslI CAYNNNNRTG 2 cut(s) 1556, 1674
Msp20I TGGCCA 1 cut(s) 890
MspA1I CMGCKG 4 cut(s) 89, 227, 2616, 2716
MspI CCGG 5 cut(s) 576, 1212, 1386, 2103, 2318
MspR9I CCNGG 9 cut(s) 450, 1386, 1836, 2104, 2319, 2375, 2417, 2427, 2806
MunI CAATTG 3 cut(s) 855, 1098, 1515
Mva1269I GAATGC 3 cut(s) 577, 1642, 2332
MvaI CCWGG 6 cut(s) 450, 1836, 2375, 2417, 2427, 2806
MvnI CGCG 2 cut(s) 1134, 1136
NciI CCSGG 3 cut(s) 1386, 2104, 2319
NcoI CCATGG 2 cut(s) 885, 2725
NdeI CATATG 1 cut(s) 242
NheI GCTAGC 1 cut(s) 1111
NlaIV GGNNCC 5 cut(s) 200, 387, 447, 2308, 2309
NmuCI GTSAC 3 cut(s) 552, 880, 2389
NsbI TGCGCA 1 cut(s) 252
NspI RCATGY 5 cut(s) 606, 653, 1222, 1561, 2575
NspV TTCGAA 1 cut(s) 69
PagI TCATGA 1 cut(s) 1438
PciI ACATGT 2 cut(s) 602, 1557
PciSI GCTCTTC 1 cut(s) 1456
PctI GAATGC 3 cut(s) 577, 1642, 2332
PdmI GAANNNNTTC 2 cut(s) 2189, 2258
PfeI GAWTC 1 cut(s) 2287
PfoI TCCNGGA 3 cut(s) 448, 2317, 2415
PinAI ACCGGT 1 cut(s) 1211
PkrI GCNGC 8 cut(s) 226, 229, 361, 670, 1096, 1801, 2698, 2707
PleI GAGTC 3 cut(s) 559, 1742, 2872
PpsI GAGTC 3 cut(s) 559, 1742, 2872
Ppu21I YACGTR 1 cut(s) 1931
PpuMI RGGWCCY 1 cut(s) 2307
PscI ACATGT 2 cut(s) 602, 1557
PshBI ATTAAT 1 cut(s) 2477
Psp124BI GAGCTC 2 cut(s) 777, 1468
Psp5II RGGWCCY 1 cut(s) 2307
Psp6I CCWGG 6 cut(s) 448, 1834, 2373, 2415, 2425, 2804
PspFI CCCAGC 1 cut(s) 959
PspGI CCWGG 6 cut(s) 448, 1834, 2373, 2415, 2425, 2804
PspN4I GGNNCC 5 cut(s) 200, 387, 447, 2308, 2309
PspPI GGNCC 4 cut(s) 1391, 1993, 2307, 2722
PspPPI RGGWCCY 1 cut(s) 2307
PsrI GAACNNNNNNTAC 1 cut(s) 2894
PstI CTGCAG 1 cut(s) 980
PstNI CAGNNNCTG 2 cut(s) 1835, 2069
PvuII CAGCTG 2 cut(s) 89, 227
RseI CAYNNNNRTG 2 cut(s) 1556, 1674
SacI GAGCTC 2 cut(s) 777, 1468
SapI GCTCTTC 1 cut(s) 1456
SaqAI TTAA 9 cut(s) 233, 315, 521, 719, 998, 1488, 2013, 2052, 2477
SatI GCNGC 8 cut(s) 225, 228, 360, 669, 1095, 1800, 2697, 2706
Sau96I GGNCC 4 cut(s) 1391, 1993, 2307, 2722
ScaI AGTACT 2 cut(s) 103, 1824
SchI GAGTC 3 cut(s) 560, 1743, 2873
ScrFI CCNGG 9 cut(s) 450, 1386, 1836, 2104, 2319, 2375, 2417, 2427, 2806
SduI GDGCHC 7 cut(s) 705, 777, 975, 1243, 1468, 1553, 1941
SexAI ACCWGGT 2 cut(s) 1834, 2425
SfcI CTRYAG 1 cut(s) 976
SfuI TTCGAA 1 cut(s) 69
SinI GGWCC 4 cut(s) 1391, 1993, 2307, 2722
SmiMI CAYNNNNRTG 2 cut(s) 1556, 1674
SmlI CTYRAG 2 cut(s) 11, 363
SmoI CTYRAG 2 cut(s) 11, 363
SnaBI TACGTA 1 cut(s) 1931
SsiI CCGC 9 cut(s) 121, 261, 537, 626, 1136, 1958, 2616, 2705, 2716
SspMI CTAG 4 cut(s) 1112, 1469, 1613, 2508
SstI GAGCTC 2 cut(s) 777, 1468
StyD4I CCNGG 9 cut(s) 448, 1384, 1834, 2102, 2317, 2373, 2415, 2425, 2804
StyI CCWWGG 4 cut(s) 885, 1394, 2040, 2725
TaaI ACNGT 7 cut(s) 871, 1018, 1303, 1382, 2075, 2140, 2722
TaiI ACGT 3 cut(s) 1438, 1933, 2815
TaqI TCGA 8 cut(s) 69, 507, 1333, 2153, 2208, 2602, 2862, 2895
TaqII GACCGA 1 cut(s) 1397
TatI WGTACW 5 cut(s) 83, 101, 646, 1822, 2203
TauI GCSGC 1 cut(s) 2708
TfiI GAWTC 1 cut(s) 2287
Tru1I TTAA 9 cut(s) 233, 315, 521, 719, 998, 1488, 2013, 2052, 2477
Tru9I TTAA 9 cut(s) 233, 315, 521, 719, 998, 1488, 2013, 2052, 2477
TscAI CASTG 4 cut(s) 763, 985, 1023, 1861
TseFI GTSAC 3 cut(s) 552, 880, 2389
TseI GCWGC 7 cut(s) 224, 227, 359, 668, 1094, 1799, 2696
Tsp45I GTSAC 3 cut(s) 552, 880, 2389
TspDTI ATGAA 5 cut(s) 775, 1638, 1907, 2198, 2339
TspGWI ACGGA 2 cut(s) 270, 2678
TspRI CASTG 4 cut(s) 763, 985, 1023, 1861
VneI GTGCAC 1 cut(s) 1239
VpaK11BI GGWCC 4 cut(s) 1391, 1993, 2307, 2722
VspI ATTAAT 1 cut(s) 2477
XapI RAATTY 8 cut(s) 73, 179, 1027, 1313, 1970, 2171, 2258, 2511
XbaI TCTAGA 1 cut(s) 1468
XceI RCATGY 5 cut(s) 606, 653, 1222, 1561, 2575
XmiI GTMKAC 2 cut(s) 588, 819
XmnI GAANNNNTTC 2 cut(s) 2189, 2258
XspI CTAG 4 cut(s) 1112, 1469, 1613, 2508
ZrmI AGTACT 2 cut(s) 103, 1824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.