RLG00000030415

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002698
Physical Location & Seq
Reverse (-)
61149161 .. 61150064
904 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030415

Sequence Viewer

Length: 630 bp
ATGGATAATGATTTCTGTAATTTACCTGAAGTACCTGCTGGAACCAAAGACAGTCCTGGTGGACCTTGGGAGTTTGTTGGTCTTCTACCTTTGTTTGATCTACCACATCACGACAGTGCTGAAACTATTAGAAGAGCTCTAGATCTTGGTGTGAGTGTTAAGATGATTACAGGCGATCAACTAGAAATTGCTAAAGAGACAGGGAGACGACTTGGGATGGGATCAAACATGTATCCATCATCTTCATTGCTTGGTGAAAATAAGAATGGATTAGATGCAAGTCTACAAATTGATGAACTCATTGAGAATGCTGATGGTTTTGCTGGTGTCTTTCCAGAGCATAAATATGAGATTGTTCAGCGATTACAAATTAAGAAGCACATAGTTGGAATGACCGGTGATGGAGTTAAGGATGCACCTGCCTTGAAGAAAGCAGACATAGGAATTGCTGTGGCAGATGCCACAGATGCTGCTCGTAGTGCGTCTGATATAGTACTGACACAGCCTGGATTGAGTGTTATTATTAGTGCTGTTCTGACAAGCCGTGCAATCTTTCAAAGAATGAAGTACTACACAGTAAGGGGATTCTCCCTTCTCATTTTTTTAGGAATTGGACCAGCCTGGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

210

Amino Acids

22.48

Weight (kDa)

5.2

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 24 - 146 8.4e-11 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
Acc36I ACCTGC 2 cut(s) 43, 427
AccI GTMKAC 1 cut(s) 283
AclWI GGATC 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 3 cut(s) 33, 495, 569
AflIII ACRYGT 1 cut(s) 228
AgeI ACCGGT 1 cut(s) 395
AgsI TTSAA 2 cut(s) 427, 557
AjnI CCWGG 3 cut(s) 55, 505, 620
AleI CACNNNNGTG 1 cut(s) 114
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw21I GWGCWC 1 cut(s) 139
Alw26I GTCTC 2 cut(s) 191, 199
AlwI GGATC 1 cut(s) 229
AlwNI CAGNNNCTG 1 cut(s) 470
ApeKI GCWGC 1 cut(s) 470
AsiGI ACCGGT 1 cut(s) 395
AspS9I GGNCC 2 cut(s) 62, 614
AsuHPI GGTGA 2 cut(s) 266, 410
AvaII GGWCC 2 cut(s) 62, 614
BanII GRGCYC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 139
BbvI GCAGC 1 cut(s) 457
BccI CCATC 4 cut(s) 211, 244, 308, 395
BceAI ACGGC 1 cut(s) 528
BciT130I CCWGG 3 cut(s) 57, 507, 622
BciVI GTATCC 1 cut(s) 243
BcoDI GTCTC 2 cut(s) 191, 199
BfaI CTAG 2 cut(s) 140, 182
BfuAI ACCTGC 2 cut(s) 43, 427
BfuI GTATCC 1 cut(s) 243
BglII AGATCT 1 cut(s) 142
BisI GCNGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 472
BmcAI AGTACT 2 cut(s) 495, 569
Bme1390I CCNGG 3 cut(s) 57, 507, 622
Bme18I GGWCC 2 cut(s) 62, 614
BmgT120I GGNCC 2 cut(s) 62, 614
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 3 cut(s) 57, 507, 622
BmsI GCATC 4 cut(s) 265, 403, 448, 457
BpiI GAAGAC 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 65
BsaWI WCCGGW 1 cut(s) 395
Bse118I RCCGGY 1 cut(s) 395
Bse3DI GCAATG 1 cut(s) 245
BseBI CCWGG 3 cut(s) 57, 507, 622
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 2 cut(s) 222, 418
BseMI GCAATG 1 cut(s) 245
BseXI GCAGC 1 cut(s) 457
BshTI ACCGGT 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 139
BsiSI CCGG 1 cut(s) 396
BsmAI GTCTC 2 cut(s) 191, 199
BsmBI CGTCTC 1 cut(s) 199
BsmI GAATGC 1 cut(s) 313
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 4 cut(s) 97, 142, 175, 221
BspLI GGNNCC 1 cut(s) 43
BspMI ACCTGC 2 cut(s) 43, 427
BspPI GGATC 1 cut(s) 229
BspQI GCTCTTC 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 245
BsrFI RCCGGY 1 cut(s) 395
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 4 cut(s) 97, 142, 175, 221
BssT1I CCWWGG 1 cut(s) 65
Bst2UI CCWGG 3 cut(s) 57, 507, 622
Bst4CI ACNGT 3 cut(s) 53, 116, 577
Bst6I CTCTTC 1 cut(s) 127
BstF5I GGATG 2 cut(s) 222, 418
BstKTI GATC 4 cut(s) 100, 145, 178, 224
BstMAI GTCTC 2 cut(s) 191, 199
BstMBI GATC 4 cut(s) 97, 142, 175, 221
BstMWI GCNNNNNNNGC 2 cut(s) 467, 479
BstNI CCWGG 3 cut(s) 57, 507, 622
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 55, 505, 620
BstV1I GCAGC 1 cut(s) 457
BstV2I GAAGAC 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
BsuI GTATCC 1 cut(s) 243
BtsCI GGATG 2 cut(s) 222, 418
BtsIMutI CAGTG 1 cut(s) 121
BveI ACCTGC 2 cut(s) 43, 427
CaiI CAGNNNCTG 1 cut(s) 470
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 2 cut(s) 62, 614
CseI GACGC 1 cut(s) 471
Csp6I GTAC 3 cut(s) 32, 494, 568
CspAI ACCGGT 1 cut(s) 395
CviAII CATG 1 cut(s) 229
CviJI RGCY 4 cut(s) 137, 505, 543, 620
CviKI_1 RGCY 4 cut(s) 137, 505, 543, 620
CviQI GTAC 3 cut(s) 32, 494, 568
DpnI GATC 4 cut(s) 99, 144, 177, 223
DpnII GATC 4 cut(s) 97, 142, 175, 221
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
Ecl136II GAGCTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 62, 614
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 137
EcoRII CCWGG 3 cut(s) 55, 505, 620
EcoT14I CCWWGG 1 cut(s) 65
EcoT38I GRGCYC 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 65
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 1 cut(s) 232
FaiI YATR 6 cut(s) 230, 342, 348, 383, 440, 491
FatI CATG 1 cut(s) 228
FblI GTMKAC 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 471
FokI GGATG 2 cut(s) 229, 425
FriOI GRGCYC 1 cut(s) 139
Fsp4HI GCNGC 1 cut(s) 471
FspBI CTAG 2 cut(s) 140, 182
GluI GCNGC 1 cut(s) 471
HapII CCGG 1 cut(s) 396
HgaI GACGC 1 cut(s) 471
Hin1II CATG 1 cut(s) 232
HinfI GANTC 1 cut(s) 585
HpaII CCGG 1 cut(s) 396
HphI GGTGA 2 cut(s) 266, 410
Hpy166II GTNNAC 2 cut(s) 62, 284
Hpy188I TCNGA 2 cut(s) 487, 537
Hpy188III TCNNGA 3 cut(s) 110, 140, 335
Hpy8I GTNNAC 2 cut(s) 62, 284
HpyAV CCTTC 1 cut(s) 602
HpyCH4III ACNGT 3 cut(s) 53, 116, 577
HpyCH4V TGCA 3 cut(s) 278, 416, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 467, 479
Hsp92II CATG 1 cut(s) 232
Kzo9I GATC 4 cut(s) 97, 142, 175, 221
LguI GCTCTTC 1 cut(s) 127
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 4 cut(s) 265, 403, 448, 457
MaeI CTAG 2 cut(s) 140, 182
MalI GATC 4 cut(s) 99, 144, 177, 223
MboI GATC 4 cut(s) 97, 142, 175, 221
MboII GAAGA 4 cut(s) 74, 144, 234, 439
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 6 cut(s) 19, 186, 288, 369, 444, 609
MmeI TCCRAC 1 cut(s) 367
MseI TTAA 3 cut(s) 159, 372, 408
MslI CAYNNNNRTG 2 cut(s) 114, 345
MspI CCGG 1 cut(s) 396
MspR9I CCNGG 3 cut(s) 57, 507, 622
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 3 cut(s) 57, 507, 622
MwoI GCNNNNNNNGC 2 cut(s) 467, 479
NdeII GATC 4 cut(s) 97, 142, 175, 221
NlaIII CATG 1 cut(s) 232
NlaIV GGNNCC 1 cut(s) 43
NspI RCATGY 1 cut(s) 232
OliI CACNNNNGTG 1 cut(s) 114
PaqCI CACCTGC 1 cut(s) 427
PciI ACATGT 1 cut(s) 228
PciSI GCTCTTC 1 cut(s) 127
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 1 cut(s) 585
PinAI ACCGGT 1 cut(s) 395
PkrI GCNGC 1 cut(s) 472
PscI ACATGT 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 139
Psp6I CCWGG 3 cut(s) 55, 505, 620
PspGI CCWGG 3 cut(s) 55, 505, 620
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 2 cut(s) 62, 614
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 1 cut(s) 142
RsaI GTAC 3 cut(s) 33, 495, 569
RsaNI GTAC 3 cut(s) 32, 494, 568
RseI CAYNNNNRTG 2 cut(s) 114, 345
SacI GAGCTC 1 cut(s) 139
SapI GCTCTTC 1 cut(s) 127
SaqAI TTAA 3 cut(s) 159, 372, 408
SatI GCNGC 1 cut(s) 471
Sau3AI GATC 4 cut(s) 97, 142, 175, 221
Sau96I GGNCC 2 cut(s) 62, 614
ScaI AGTACT 2 cut(s) 495, 569
ScrFI CCNGG 3 cut(s) 57, 507, 622
SduI GDGCHC 1 cut(s) 139
SetI ASST 6 cut(s) 28, 37, 67, 91, 139, 421
SfaNI GCATC 4 cut(s) 265, 403, 448, 457
SinI GGWCC 2 cut(s) 62, 614
SmiMI CAYNNNNRTG 2 cut(s) 114, 345
Sse9I AATT 6 cut(s) 19, 186, 288, 369, 444, 609
SspMI CTAG 2 cut(s) 140, 182
SstI GAGCTC 1 cut(s) 139
StyD4I CCNGG 3 cut(s) 55, 505, 620
StyI CCWWGG 1 cut(s) 65
TaaI ACNGT 3 cut(s) 53, 116, 577
TasI AATT 6 cut(s) 19, 186, 288, 369, 444, 609
TatI WGTACW 2 cut(s) 493, 567
TfiI GAWTC 1 cut(s) 585
Tru1I TTAA 3 cut(s) 159, 372, 408
Tru9I TTAA 3 cut(s) 159, 372, 408
TscAI CASTG 1 cut(s) 121
TseI GCWGC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 234, 309, 578
TspRI CASTG 1 cut(s) 121
VpaK11BI GGWCC 2 cut(s) 62, 614
XbaI TCTAGA 1 cut(s) 139
XceI RCATGY 1 cut(s) 232
XmiI GTMKAC 1 cut(s) 283
XspI CTAG 2 cut(s) 140, 182
ZrmI AGTACT 2 cut(s) 495, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.