MD03G1197700.v1.1

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002698
Physical Location & Seq
Reverse (-)
27042382 .. 27044784
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1197700.v1.1.491

Sequence Viewer

Length: 1365 bp
ATGTATCCATCATCGGCGTTGCTTGGTGAAAATAAGAATAGCATAGATGGAACTCTAGTAATTGATGAACTCATTGAGAATGCTGATGGTTTTGCTGGTGTATTTCCTGAACATAAATATGAGATCGTTCAGCGATTACAAGGTAAGAAGCACATAGTCGGAATGACAGGTGATGGAGTCAATGATGCACCAGCATTGAAGAAAGCAGACATAGGAATTGCTGTCGCCGATGCCACAGATGCAGCCCGTGGTGCATCTGATATAGTACTGACAGAACCTGGTTTGAGTGTGATTATCAGTGCTGTTTTGACAAGCCGTGCAATCTTTCAAAGAATGAAGAATTACACAATATACGCAGTATCTATAACAATACGTATTGTGCTCGGTTTCTTGTTGCTTGCGGTATTCTGGAAATTTGATTTCCCTCCTTTTATGGTCCTAATCATTGCCATTCTTAATGATGGTACTATTATGACGATATCCAAGGACAGGGTTAAGCCATCTCCAGTTCCTGACAGTTGGAAGCTGAGTGAAATCTTTGCAACCGGGATTGTGTTAGGCAGTTATCTTGCCGTTACCACAGTTATATTCTTCTCGATCATTTACGATACTAAATTTTTTCCGATGAAATTGTTCTCACATTTTGTACTCGATTACAATGATCAAGTTTTCACCTTCTCTCGTTCTCTCTCGCAGAAAAAATTCGGTGTAGAAGATATCTTCAATGAGAATCTCTATTATACCAACGGGACCCTCAATCCGGGAATGAATGCCAAGCTATCATCTGCTCTGTACCTTCAAGTTAGCACCATCAGCCAGGCTCTAATTTTTGTCACTCGCTCCCGAGGATGGTCATTCCTGGAAGTACCTGGTCTGCTTCTGCTTAGTGCTTTCGTAATTGCTCAACTGATTGCTACATTAATATCTGCTTTAGTAACATGGAAAATTGCTAGAATTCAAGCAATCGGCTGGAAATGGTGTGGTGTCGTATGGATATACAACATTCTAATCTACATGCTACTTGATCCTGTCAAGATTTTCGTTCGATATGCACTCAGCGGGAGGGCTTGGAGTTTGGTATTGAACAAAAGAACGGCTTTCAACACCCAAAAGGACTTCGGTAAAGAGTTCCGTGAGGCTGCATGGGCGGCAGAACAGCGGACGGTCCATGGCCTCCCATCTATGGAGGCAAAGAACATTCCTGAGAGGCACACATTCAGGGATGTTAGCATCATGGCTGAAGAAGCCAGGAGACGTGCAGAGATTGCAAGACTAAGGGAGCTTCACACTCTGAAGGGGAAGGTCGAGTCCTTTGCAAAGCTAAGGGGTTTAGACATCGAAACGAACCCAAATTACACTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

455

Amino Acids

50.85

Weight (kDa)

9.19

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 28 - 70 4.8e-06 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 401, 1059, 1148, 1159
AclWI GGATC 1 cut(s) 1019
AcsI RAATTY 4 cut(s) 413, 614, 701, 954
AcuI CTGAAG 2 cut(s) 1260, 1313
AfaI GTAC 5 cut(s) 267, 466, 648, 794, 867
AfiI CCNNNNNNNGG 4 cut(s) 489, 760, 849, 1183
AgsI TTSAA 7 cut(s) 199, 329, 724, 800, 959, 1084, 1102
AjiI CACGTC 1 cut(s) 1256
AjnI CCWGG 5 cut(s) 277, 816, 858, 868, 1247
AluBI AGCT 4 cut(s) 526, 778, 1282, 1321
AluI AGCT 4 cut(s) 526, 778, 1282, 1321
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 1 cut(s) 1246
AlwI GGATC 1 cut(s) 1019
AlwNI CAGNNNCTG 2 cut(s) 278, 512
Ama87I CYCGRG 1 cut(s) 843
AoxI GGCC 1 cut(s) 1171
ApeKI GCWGC 2 cut(s) 242, 1139
ApoI RAATTY 4 cut(s) 413, 614, 701, 954
AseI ATTAAT 1 cut(s) 920
Asp700I GAANNNNTTC 2 cut(s) 632, 701
AspS9I GGNCC 3 cut(s) 436, 750, 1165
AsuC2I CCSGG 2 cut(s) 547, 762
AsuHPI GGTGA 3 cut(s) 38, 182, 664
AvaI CYCGRG 1 cut(s) 843
AvaII GGWCC 3 cut(s) 436, 750, 1165
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 254, 1126
BccI CCATC 9 cut(s) 16, 41, 80, 167, 455, 508, 818, 843, 1186
BceAI ACGGC 3 cut(s) 300, 557, 1110
BcgI CGANNNNNNTGC 2 cut(s) 1295, 1329
BciT130I CCWGG 5 cut(s) 279, 818, 860, 870, 1249
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 661
BcnI CCSGG 2 cut(s) 547, 762
BcoDI GTCTC 1 cut(s) 1246
BfaI CTAG 2 cut(s) 56, 951
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 3 cut(s) 243, 1140, 1149
BlsI GCNGC 3 cut(s) 244, 1141, 1150
BmcAI AGTACT 1 cut(s) 267
Bme1390I CCNGG 7 cut(s) 279, 547, 762, 818, 860, 870, 1249
Bme18I GGWCC 3 cut(s) 436, 750, 1165
BmeT110I CYCGRG 1 cut(s) 843
BmgBI CACGTC 1 cut(s) 1256
BmgT120I GGNCC 3 cut(s) 436, 750, 1165
BmiI GGNNCC 2 cut(s) 751, 752
BmrFI CCNGG 7 cut(s) 279, 547, 762, 818, 860, 870, 1249
BmsI GCATC 5 cut(s) 175, 220, 229, 263, 1239
BpmI CTGGAG 1 cut(s) 489
Bpu10I CCTNAGC 1 cut(s) 1322
BpuMI CCSGG 2 cut(s) 547, 762
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 4 cut(s) 247, 483, 844, 1168
Bsc4I CCNNNNNNNGG 4 cut(s) 489, 760, 849, 1183
Bse1I ACTGG 1 cut(s) 506
Bse3DI GCAATG 1 cut(s) 444
BseBI CCWGG 5 cut(s) 279, 818, 860, 870, 1249
BseDI CCNNGG 4 cut(s) 247, 483, 844, 1168
BseGI GGATG 2 cut(s) 854, 1228
BseLI CCNNNNNNNGG 4 cut(s) 489, 760, 849, 1183
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 3 cut(s) 518, 1069, 1194
BseNI ACTGG 1 cut(s) 506
BseXI GCAGC 2 cut(s) 254, 1126
BsgI GTGCAG 1 cut(s) 1278
BshFI GGCC 1 cut(s) 1173
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 1 cut(s) 843
BsiSI CCGG 2 cut(s) 546, 761
BslFI GGGAC 1 cut(s) 763
BslI CCNNNNNNNGG 4 cut(s) 489, 760, 849, 1183
BsmAI GTCTC 1 cut(s) 1246
BsmBI CGTCTC 1 cut(s) 1246
BsmFI GGGAC 1 cut(s) 763
BsmI GAATGC 2 cut(s) 85, 775
BsnI GGCC 1 cut(s) 1173
BsoBI CYCGRG 1 cut(s) 843
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 4 cut(s) 123, 597, 661, 1024
Bsp19I CCATGG 1 cut(s) 1168
BspACI CCGC 4 cut(s) 401, 1059, 1148, 1159
BspANI GGCC 1 cut(s) 1173
BspCNI CTCAG 3 cut(s) 519, 1068, 1195
BspLI GGNNCC 2 cut(s) 751, 752
BspPI GGATC 1 cut(s) 1019
BsrDI GCAATG 1 cut(s) 444
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 4 cut(s) 247, 483, 844, 1168
BssMI GATC 4 cut(s) 123, 597, 661, 1024
BssT1I CCWWGG 2 cut(s) 483, 1168
Bst2UI CCWGG 5 cut(s) 279, 818, 860, 870, 1249
Bst4CI ACNGT 3 cut(s) 518, 583, 1165
BstAPI GCANNNNNTGC 1 cut(s) 1265
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 399
BstDEI CTNAG 6 cut(s) 527, 884, 1055, 1203, 1274, 1322
BstDSI CCRYGG 2 cut(s) 247, 1168
BstF5I GGATG 2 cut(s) 854, 1228
BstKTI GATC 4 cut(s) 126, 600, 664, 1027
BstMAI GTCTC 1 cut(s) 1246
BstMBI GATC 4 cut(s) 123, 597, 661, 1024
BstMWI GCNNNNNNNGC 7 cut(s) 239, 251, 813, 1145, 1148, 1244, 1265
BstNI CCWGG 5 cut(s) 279, 818, 860, 870, 1249
BstNSI RCATGY 1 cut(s) 1018
BstSCI CCNGG 7 cut(s) 277, 545, 760, 816, 858, 868, 1247
BstSNI TACGTA 1 cut(s) 374
BstV1I GCAGC 2 cut(s) 254, 1126
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 1173
BtgI CCRYGG 2 cut(s) 247, 1168
BtrI CACGTC 1 cut(s) 1256
BtsCI GGATG 2 cut(s) 854, 1228
BtsIMutI CAGTG 1 cut(s) 304
Cac8I GCNNGC 1 cut(s) 399
CaiI CAGNNNCTG 2 cut(s) 278, 512
Cfr13I GGNCC 3 cut(s) 436, 750, 1165
CsiI ACCWGGT 2 cut(s) 277, 868
Csp6I GTAC 5 cut(s) 266, 465, 647, 793, 866
CviAII CATG 5 cut(s) 939, 1015, 1143, 1169, 1234
CviQI GTAC 5 cut(s) 266, 465, 647, 793, 866
DdeI CTNAG 6 cut(s) 527, 884, 1055, 1203, 1274, 1322
DpnI GATC 4 cut(s) 125, 599, 663, 1026
DpnII GATC 4 cut(s) 123, 597, 661, 1024
Eco105I TACGTA 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 483, 1168
Eco32I GATATC 2 cut(s) 480, 718
Eco47I GGWCC 3 cut(s) 436, 750, 1165
Eco57I CTGAAG 2 cut(s) 1260, 1313
Eco88I CYCGRG 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 750
EcoRI GAATTC 1 cut(s) 954
EcoRII CCWGG 5 cut(s) 277, 816, 858, 868, 1247
EcoRV GATATC 2 cut(s) 480, 718
EcoT14I CCWWGG 2 cut(s) 483, 1168
ErhI CCWWGG 2 cut(s) 483, 1168
Esp3I CGTCTC 1 cut(s) 1246
FaeI CATG 5 cut(s) 942, 1018, 1146, 1172, 1237
FalI AAGNNNNNCTT 2 cut(s) 1081, 1113
FaqI GGGAC 1 cut(s) 763
FatI CATG 5 cut(s) 938, 1014, 1142, 1168, 1233
FauI CCCGC 1 cut(s) 1052
FbaI TGATCA 1 cut(s) 661
Fnu4HI GCNGC 3 cut(s) 243, 1140, 1149
FokI GGATG 2 cut(s) 861, 1235
Fsp4HI GCNGC 3 cut(s) 243, 1140, 1149
FspBI CTAG 2 cut(s) 56, 951
GluI GCNGC 3 cut(s) 243, 1140, 1149
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 1 cut(s) 1173
HapII CCGG 2 cut(s) 546, 761
Hin1II CATG 5 cut(s) 942, 1018, 1146, 1172, 1237
HinfI GANTC 3 cut(s) 177, 730, 1307
HpaII CCGG 2 cut(s) 546, 761
HphI GGTGA 3 cut(s) 38, 182, 664
Hpy188I TCNGA 4 cut(s) 161, 259, 624, 1293
Hpy188III TCNNGA 7 cut(s) 107, 409, 512, 595, 843, 1033, 1202
HpyAV CCTTC 4 cut(s) 685, 806, 1288, 1294
HpyCH4III ACNGT 3 cut(s) 518, 583, 1165
HpyCH4IV ACGT 2 cut(s) 373, 1255
HpyF10VI GCNNNNNNNGC 7 cut(s) 239, 251, 813, 1145, 1148, 1244, 1265
HpyF3I CTNAG 6 cut(s) 527, 884, 1055, 1203, 1274, 1322
HpySE526I ACGT 2 cut(s) 373, 1255
Hsp92II CATG 5 cut(s) 942, 1018, 1146, 1172, 1237
KflI GGGWCCC 1 cut(s) 750
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 4 cut(s) 123, 597, 661, 1024
LmnI GCTCC 2 cut(s) 845, 1279
Lsp1109I GCAGC 2 cut(s) 254, 1126
LweI GCATC 5 cut(s) 175, 220, 229, 263, 1239
MabI ACCWGGT 2 cut(s) 277, 868
MaeI CTAG 2 cut(s) 56, 951
MaeII ACGT 2 cut(s) 373, 1255
MaeIII GTNAC 3 cut(s) 574, 832, 934
MalI GATC 4 cut(s) 125, 599, 663, 1026
MboI GATC 4 cut(s) 123, 597, 661, 1024
MboII GAAGA 6 cut(s) 211, 349, 583, 712, 725, 1253
MhlI GDGCHC 1 cut(s) 384
MlyI GAGTC 2 cut(s) 186, 1316
MmeI TCCRAC 2 cut(s) 139, 500
MnlI CCTC 8 cut(s) 435, 764, 839, 1056, 1129, 1180, 1184, 1200
MroXI GAANNNNTTC 2 cut(s) 632, 701
MseI TTAA 3 cut(s) 456, 495, 920
MslI CAYNNNNRTG 1 cut(s) 117
MspA1I CMGCKG 2 cut(s) 1059, 1159
MspI CCGG 2 cut(s) 546, 761
MspR9I CCNGG 7 cut(s) 279, 547, 762, 818, 860, 870, 1249
Mva1269I GAATGC 2 cut(s) 85, 775
MvaI CCWGG 5 cut(s) 279, 818, 860, 870, 1249
MwoI GCNNNNNNNGC 7 cut(s) 239, 251, 813, 1145, 1148, 1244, 1265
NciI CCSGG 2 cut(s) 547, 762
NcoI CCATGG 1 cut(s) 1168
NdeII GATC 4 cut(s) 123, 597, 661, 1024
NlaIII CATG 5 cut(s) 942, 1018, 1146, 1172, 1237
NlaIV GGNNCC 2 cut(s) 751, 752
NmuCI GTSAC 1 cut(s) 832
NspI RCATGY 1 cut(s) 1018
PctI GAATGC 2 cut(s) 85, 775
PdmI GAANNNNTTC 2 cut(s) 632, 701
PfeI GAWTC 1 cut(s) 730
PfoI TCCNGGA 2 cut(s) 760, 858
PkrI GCNGC 3 cut(s) 244, 1141, 1150
PleI GAGTC 2 cut(s) 185, 1315
PpsI GAGTC 2 cut(s) 185, 1315
Ppu21I YACGTR 1 cut(s) 374
PpuMI RGGWCCY 1 cut(s) 750
PshBI ATTAAT 1 cut(s) 920
Psp5II RGGWCCY 1 cut(s) 750
Psp6I CCWGG 5 cut(s) 277, 816, 858, 868, 1247
PspGI CCWGG 5 cut(s) 277, 816, 858, 868, 1247
PspN4I GGNNCC 2 cut(s) 751, 752
PspPI GGNCC 3 cut(s) 436, 750, 1165
PspPPI RGGWCCY 1 cut(s) 750
PsrI GAACNNNNNNTAC 1 cut(s) 1337
PstNI CAGNNNCTG 2 cut(s) 278, 512
RsaI GTAC 5 cut(s) 267, 466, 648, 794, 867
RsaNI GTAC 5 cut(s) 266, 465, 647, 793, 866
RseI CAYNNNNRTG 1 cut(s) 117
SaqAI TTAA 3 cut(s) 456, 495, 920
SatI GCNGC 3 cut(s) 243, 1140, 1149
Sau3AI GATC 4 cut(s) 123, 597, 661, 1024
Sau96I GGNCC 3 cut(s) 436, 750, 1165
ScaI AGTACT 1 cut(s) 267
SchI GAGTC 2 cut(s) 186, 1316
ScrFI CCNGG 7 cut(s) 279, 547, 762, 818, 860, 870, 1249
SduI GDGCHC 1 cut(s) 384
SexAI ACCWGGT 2 cut(s) 277, 868
SfaNI GCATC 5 cut(s) 175, 220, 229, 263, 1239
SinI GGWCC 3 cut(s) 436, 750, 1165
SmiMI CAYNNNNRTG 1 cut(s) 117
SnaBI TACGTA 1 cut(s) 374
SsiI CCGC 4 cut(s) 401, 1059, 1148, 1159
SspMI CTAG 2 cut(s) 56, 951
StyD4I CCNGG 7 cut(s) 277, 545, 760, 816, 858, 868, 1247
StyI CCWWGG 2 cut(s) 483, 1168
TaaI ACNGT 3 cut(s) 518, 583, 1165
TaiI ACGT 2 cut(s) 376, 1258
TaqI TCGA 5 cut(s) 596, 651, 1045, 1305, 1338
TatI WGTACW 2 cut(s) 265, 646
TauI GCSGC 1 cut(s) 1151
TfiI GAWTC 1 cut(s) 730
Tru1I TTAA 3 cut(s) 456, 495, 920
Tru9I TTAA 3 cut(s) 456, 495, 920
TscAI CASTG 1 cut(s) 304
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 2 cut(s) 242, 1139
Tsp45I GTSAC 1 cut(s) 832
TspDTI ATGAA 4 cut(s) 81, 350, 641, 782
TspGWI ACGGA 1 cut(s) 1121
TspRI CASTG 1 cut(s) 304
VpaK11BI GGWCC 3 cut(s) 436, 750, 1165
VspI ATTAAT 1 cut(s) 920
XapI RAATTY 4 cut(s) 413, 614, 701, 954
XceI RCATGY 1 cut(s) 1018
XmnI GAANNNNTTC 2 cut(s) 632, 701
XspI CTAG 2 cut(s) 56, 951
ZrmI AGTACT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.