Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002698

Physical Location & Seq

Genomic Coordinates Forward (+)

47061653 .. 47067757

Gene Structure 6105 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00252980.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGGATACATCAATTGCCCTTCAGCCTATTACGCAGGAAGCTGTTGATCTGGAGAACATCCCTGTGGAGGAAGTTTTTGAACAATTGAAATGTACAGCAGAGGGTCTCAATTCTGAGGAGGTGCAAAAGCGTCTGGAGGTTTTTGGTTTCAACAAACTTCAAGAGAAAAAGGGGAGAAAAAGAGACGTTGAGGATTTCTGTGGAATCCTAGCCCTACTCCTTATCAATTCAACAATAAGCTTTATCGAGGAAAACAATGCTGGAAATGCAGCTGCAGCGCTTATGGCCCGGCTTGCTCCAAAAGCCAAGGTTTTACGCGATGGGAAATGGAATGAGGAAGATGCAGCTGTGCTAGTTCCTGGAGACATTATCAGTATCAAGTTAGGAGATATAATTCCTGCTGATGCTCGCCTGCTTCAAGGAGATCCTTTGAAGATCGACCAGTCTGCTCTTACAGGAGAGTCTCTCCCGGTAACTAAGCATGCCGGTGAAGGAGTATACTCTGGCTCAACATGTAAACAAGGTGAAATTGAAGCAGTTGTCATCGCAACAGGAGTACATACCTTCTTTGGAAAAGCTGCTCATCTTGTGGAAAACACAACACATGTTGGGCACTTTCAGAAGGTCTTAACAGCCATTGGAAACTTCTGCATCTGCTCAATTGCCATAGGTATTGTGATTGAACTAATTGTCCTAGTTCGTCAAGGAAAGGGTTATCGTTCTAAAATAGATAACCTTCTTGTCCTACTTATTGGCGGCATCCCAATTGCTATGCCAACTGTTCTCTCTGTCACCATGGCCATTGGTTCTCATCGTTTGTCTCAGCAGGGTGCAATCACAAAGAGAATGACAGCCATTGAAGAAATGGCTGGGATGGATGTGCTCTGCAGTGATAAAACAGGAACTTTAACTCTCAACAAGTTAACAGTGGACAAGAATCTGATAGAGGTGTTTGCAAAAGATGTTGAAAAAGACTCGGTTGTATTGATGGCTGCAAGAGCTTCAAGGTTAGAAAATCAAGATGCCATTGATGCAGCAATTGTTGCAATGCTGGCTGATTCTAAGGAGGCACGAGAGGGAATTACAGAAGTTCACTTCCTTCCTTTCAATCCAACTGATAAGAGGACAGCACTTACATACCTAGACAAAGCTGGTAAAATGCACAGAGTGAGCAAAGGTGCACCAGAGCAGATACTCAATTTGGCATGTAACAAATCAGATATTGAAAAGAGGGCGCACACAGTAATTGACAAATTTGCAGAACGTGGTCTTCGATCCCTTGCTGTTGCACGACAGGAAGTACCTGCTGGAACCAAAGACAGTCCTGGTGGACCTTGGGAGTTTGTTGGTCTTCTACCTTTGTTTGATCCACCACGACACGACAGTGCTGAAACTATTAGAAGAGCTCTAGATCTTGGTGTGAGTGTTAAGATGATTACAGGCGATCAACTGGCAATTGCCAAAGAGACAGGGAGACGACTTGGGATGGGCTCAAACATGTATCCATCATCTTCATTGCTTGGTGAAAATAAGAATGGCTTAGATGCAAGTCTACAAATTGATGAACTCATTGAGAATGCTGATGGTTTTGCTGGTGTCTTTCCAGAGCATAAATATGAGATCGTTCAGCGATTACAAATTAAGAAGCACATAGTTGGAATGACCGGTGATGGAGTTAATGATGCACCTGCCTTGAAGAAAGCAGACATAGGAATTGCTGTGGCAGATGCCACAGATGCTGCTCGTAGTGCATCTGATATAGTACTGACAGAGCCTGGATTGAGTGTTATTATTAGTGCTGTTCTGACAAGCCGTGCAATCTTTCAAAGAATGAAGAACTACACAATATATGCAGTGTCTATAACAATACGTATTGTGCTTGGTTTTTTGTTGCTAACTGCATTTTGGCGATTTGATTTCCCTCCTTTTATGGTCCTCATCATAGCCATTCTTAATGACGGTACTATTATGACAATATCCAAAGACAAGGTTAAGCCATCTCCAGTTCCTGACAGTTGGAAGCTCAGTGAAATATTTGCAACTGGGATTGTCTTAGGGAGTTACCTTGCCGTGACCACAGCTATATTTTTCTATATCATTTACGAAACCCACTTCTTCCCGAAACACTTCCATCTAGACCTGGACTTCAAGAAACTCGCTGATCTCAATGAGTCGAGTTATACAGAGACACTGAATGGCCAGCTAGCATCTGTTGTGTACCTTCAAGTTAGCACCATCAGCCAGGCACTAATTTTTGTAACTCGCTCCAGAGGTTGGTCATTCATGGAAAGACCTGGTCTTCTTCTTGTTGGTGCTTTCATAGTTGCTCAAATGGTTGCAACAGTAATAACTGCTCAGGCAACATGGAAATTTGCTCAAATTCATAGTGTTGGCTGGGGCTGGACTGGAGCTGTATGGATTTACAACATTATAATCTATATGCTTCTTGATCCTATCAAGTTTTTTGTGCGATATGCACTCAGTGGGAGGGCCTGGAGTTTGGTACTGAACAAAAGGACGGCTTTCACCACCCAAAAGGACTTTGGTAGGGAAGCTCGTGAGGCTGCTTGGGCAACAGAACAGCGTACACTTCATGGTCTCCCAACTATGGACAGACATGTCTTCCATGAGAGGCACACATTCAGGGATGTTAGCATGATGGCTGAAGAAGCCCGACGACGTGCAGAGATTGCAAGACTAAGGGAAATTCACACTCTCAAAGGGAAGGTGGAGTCCTTTGCAAAGCTAAGGGGTTTGGACATTGACGTCAATCCACATTACACAGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

929

Amino Acids

102.18

Weight (kDa)

6.65

Isoelectric Point (pI)

38.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	19 - 59	2.7e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	98 - 198	7e-31	P-type ATPase actuator domain
Hydrolase	PF00702	292 - 569	6.6e-15	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002698)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60330 AT3G60330 AT3G60330 AT3G60330
fragaria_vesca	FvH4_5g19790 FvH4_7g09980
malus_domestica	MD02G1216000.v1.1 MD03G1197700.v1.1 MD03G1197800.v1.1 MD04G1058300.v1.1
prunus_persica	Prupe.2G128800_v2.0.a1 Prupe.5G062200_v2.0.a1
pyrus_communis	pycom04g05210
rosa_chinensis	RchiOBHm_Chr1g0347581 RchiOBHm_Chr4g0412601 RchiOBHm_Chr7g0204571
rosa_laevigata	RLG00000003448 RLG00000028736 RLG00000030415
rosa_multiflora	Rmu_sc0001211.1_g000115 Rmu_sc0005397.1_g000001 Rmu_sc0007327.1_g000001 Rmu_sc0021384.1_g000002 Rmu_sc0024460.1_g000001 Rmu_sc0027638.1_g000006
rosa_roxburghii	Rroxscaffold_3G00252980 Rroxscaffold_4G00307640 Rroxscaffold_4G00307710
rosa_rugosa	Rorug01G0188000 Rorug07G0085200
rosa_samantha	Rh1DG200900 Rh7AG214600 Rh7BG212100 Rh7CG227700 Rh7DG222300
rosa_wichuraiana	Rw0G013650 Rw0G018200 Rw1G017220 Rw7G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2432
AarI	CACCTGC	1 cut(s)	1696
AasI	GACNNNNNNGTC	2 cut(s)	2618, 2765
AatII	GACGTC	1 cut(s)	2769
Acc36I	ACCTGC	2 cut(s)	1312, 1696
AccB7I	CCANNNNNTGG	1 cut(s)	2274
AccI	GTMKAC	2 cut(s)	498, 1552
AccII	CGCG	1 cut(s)	318
AciI	CCGC	1 cut(s)	756
AclWI	GGATC	4 cut(s)	419, 1269, 1361, 2444
AcoI	YGGCCR	2 cut(s)	798, 2197
AcsI	RAATTY	4 cut(s)	1253, 2369, 2379, 2706
AcuI	CTGAAG	2 cut(s)	5, 2685
AcyI	GRCGYC	1 cut(s)	2766
AdeI	CACNNNGTG	2 cut(s)	1168, 2483
AfaI	GTAC	8 cut(s)	94, 558, 1302, 1764, 1963, 2219, 2505, 2587
AfeI	AGCGCT	1 cut(s)	279
AfiI	CCNNNNNNNGG	3 cut(s)	67, 2274, 2608
AflIII	ACRYGT	4 cut(s)	512, 604, 1497, 2617
AgeI	ACCGGT	1 cut(s)	1664
AjiI	CACGTC	1 cut(s)	2681
AjnI	CCWGG	7 cut(s)	358, 1324, 1774, 2139, 2241, 2293, 2492
AleI	CACNNNNGTG	1 cut(s)	1383
AloI	GAACNNNNNNTCC	2 cut(s)	675, 707
Alw21I	GWGCWC	3 cut(s)	885, 1183, 1408
Alw26I	GTCTC	9 cut(s)	110, 177, 357, 468, 825, 1460, 1468, 2180, 2603
Alw44I	GTGCAC	1 cut(s)	1179
AlwI	GGATC	4 cut(s)	419, 1269, 1361, 2444
AlwNI	CAGNNNCTG	3 cut(s)	1739, 1775, 2009
Aor51HI	AGCGCT	1 cut(s)	279
AoxI	GGCC	4 cut(s)	285, 798, 2197, 2490
ApaLI	GTGCAC	1 cut(s)	1179
ApeKI	GCWGC	9 cut(s)	269, 272, 275, 344, 578, 992, 1034, 1739, 2564
ApoI	RAATTY	4 cut(s)	1253, 2369, 2379, 2706
AsiGI	ACCGGT	1 cut(s)	1664
Asp700I	GAANNNNTTC	1 cut(s)	2126
AspLEI	GCGC	2 cut(s)	280, 1237
AspS9I	GGNCC	4 cut(s)	286, 1331, 1933, 2490
AsuC2I	CCSGG	2 cut(s)	289, 470
AsuHPI	GGTGA	6 cut(s)	500, 536, 784, 1535, 1679, 2518
AsuNHI	GCTAGC	1 cut(s)	2203
AvaII	GGWCC	2 cut(s)	1331, 1933
BaeGI	GKGCMC	2 cut(s)	615, 1183
BalI	TGGCCA	2 cut(s)	800, 2199
BanII	GRGCYC	2 cut(s)	1408, 1493
BauI	CACGAG	2 cut(s)	1071, 2556
BbsI	GAAGAC	4 cut(s)	1262, 1343, 2291, 2614
Bbv12I	GWGCWC	3 cut(s)	885, 1183, 1408
BbvI	GCAGC	9 cut(s)	259, 281, 287, 356, 565, 979, 1046, 1726, 2551
BceAI	ACGGC	3 cut(s)	1797, 2054, 2535
BcgI	CGANNNNNNTGC	2 cut(s)	428, 462
BciT130I	CCWGG	7 cut(s)	360, 1326, 1776, 2141, 2243, 2295, 2494
BciVI	GTATCC	1 cut(s)	1512
BcnI	CCSGG	2 cut(s)	289, 470
BcoDI	GTCTC	9 cut(s)	110, 177, 357, 468, 825, 1460, 1468, 2180, 2603
BfaI	CTAG	7 cut(s)	209, 353, 695, 1142, 1409, 2135, 2204
BfmI	CTRYAG	2 cut(s)	273, 886
BfoI	RGCGCY	1 cut(s)	281
BfuAI	ACCTGC	2 cut(s)	1312, 1696
BfuI	GTATCC	1 cut(s)	1512
BglII	AGATCT	1 cut(s)	1411
BmcAI	AGTACT	1 cut(s)	1764
Bme1390I	CCNGG	9 cut(s)	289, 360, 470, 1326, 1776, 2141, 2243, 2295, 2494
Bme18I	GGWCC	2 cut(s)	1331, 1933
BmgBI	CACGTC	1 cut(s)	2681
BmgT120I	GGNCC	4 cut(s)	286, 1331, 1933, 2490
BmiI	GGNNCC	1 cut(s)	1312
BmrFI	CCNGG	9 cut(s)	289, 360, 470, 1326, 1776, 2141, 2243, 2295, 2494
BmrI	ACTGGG	1 cut(s)	2052
BmtI	GCTAGC	1 cut(s)	2207
BmuI	ACTGGG	1 cut(s)	2052
BpiI	GAAGAC	4 cut(s)	1262, 1343, 2291, 2614
BplI	GAGNNNNNCTC	2 cut(s)	450, 482
BpmI	CTGGAG	7 cut(s)	71, 155, 381, 1986, 2251, 2427, 2515
Bpu10I	CCTNAGC	2 cut(s)	2355, 2747
BpuMI	CCSGG	2 cut(s)	289, 470
BsaAI	YACGTR	1 cut(s)	1871
BsaHI	GRCGYC	1 cut(s)	2766
BsaI	GGTCTC	2 cut(s)	110, 2603
BsaJI	CCNNGG	3 cut(s)	306, 795, 1334
BsaWI	WCCGGW	1 cut(s)	1664
Bsc4I	CCNNNNNNNGG	3 cut(s)	67, 2274, 2608
Bse118I	RCCGGY	2 cut(s)	485, 1664
Bse1I	ACTGG	5 cut(s)	442, 1455, 2003, 2047, 2410
Bse3DI	GCAATG	2 cut(s)	1053, 1514
BseBI	CCWGG	7 cut(s)	360, 1326, 1776, 2141, 2243, 2295, 2494
BseDI	CCNNGG	3 cut(s)	306, 795, 1334
BseGI	GGATG	6 cut(s)	57, 759, 879, 883, 1491, 2653
BseLI	CCNNNNNNNGG	3 cut(s)	67, 2274, 2608
BseMI	GCAATG	2 cut(s)	1053, 1514
BseMII	CTCAG	5 cut(s)	105, 836, 2038, 2369, 2494
BseNI	ACTGG	5 cut(s)	442, 1455, 2003, 2047, 2410
BseRI	GAGGAG	1 cut(s)	131
BseSI	GKGCMC	2 cut(s)	615, 1183
BseXI	GCAGC	9 cut(s)	259, 281, 287, 356, 565, 979, 1046, 1726, 2551
BseYI	CCCAGC	2 cut(s)	869, 2394
BsgI	GTGCAG	1 cut(s)	2703
Bsh1236I	CGCG	1 cut(s)	318
BshFI	GGCC	4 cut(s)	287, 800, 2199, 2492
BshTI	ACCGGT	1 cut(s)	1664
BsiHKAI	GWGCWC	3 cut(s)	885, 1183, 1408
BsiSI	CCGG	4 cut(s)	289, 470, 486, 1665
BslI	CCNNNNNNNGG	3 cut(s)	67, 2274, 2608
BsmAI	GTCTC	9 cut(s)	110, 177, 357, 468, 825, 1460, 1468, 2180, 2603
BsmBI	CGTCTC	2 cut(s)	177, 1468
BsmI	GAATGC	1 cut(s)	1582
BsnI	GGCC	4 cut(s)	287, 800, 2199, 2492
Bso31I	GGTCTC	2 cut(s)	110, 2603
Bsp1286I	GDGCHC	5 cut(s)	615, 885, 1183, 1408, 1493
Bsp1407I	TGTACA	1 cut(s)	92
Bsp19I	CCATGG	1 cut(s)	795
BspACI	CCGC	1 cut(s)	756
BspANI	GGCC	4 cut(s)	287, 800, 2199, 2492
BspCNI	CTCAG	5 cut(s)	106, 835, 2037, 2368, 2493
BspFNI	CGCG	1 cut(s)	318
BspLI	GGNNCC	1 cut(s)	1312
BspMAI	CTGCAG	2 cut(s)	277, 890
BspMI	ACCTGC	2 cut(s)	1312, 1696
BspOI	GCTAGC	1 cut(s)	2207
BspPI	GGATC	4 cut(s)	419, 1269, 1361, 2444
BspQI	GCTCTTC	1 cut(s)	1396
BspTNI	GGTCTC	2 cut(s)	110, 2603
BsrDI	GCAATG	2 cut(s)	1053, 1514
BsrFI	RCCGGY	2 cut(s)	485, 1664
BsrGI	TGTACA	1 cut(s)	92
BsrI	ACTGG	5 cut(s)	442, 1455, 2003, 2047, 2410
BssAI	RCCGGY	2 cut(s)	485, 1664
BssECI	CCNNGG	3 cut(s)	306, 795, 1334
BssNAI	GTATAC	1 cut(s)	499
BssNI	GRCGYC	1 cut(s)	2766
BssSI	CACGAG	2 cut(s)	1071, 2556
BssT1I	CCWWGG	3 cut(s)	306, 795, 1334
Bst1107I	GTATAC	1 cut(s)	499
Bst2BI	CACGAG	2 cut(s)	1071, 2556
Bst2UI	CCWGG	7 cut(s)	360, 1326, 1776, 2141, 2243, 2295, 2494
Bst4CI	ACNGT	9 cut(s)	781, 928, 1243, 1322, 1385, 1961, 2015, 2344, 2785
Bst6I	CTCTTC	1 cut(s)	1396
BstACI	GRCGYC	1 cut(s)	2766
BstAPI	GCANNNNNTGC	2 cut(s)	1043, 2690
BstAUI	TGTACA	1 cut(s)	92
BstBAI	YACGTR	1 cut(s)	1871
BstC8I	GCNNGC	7 cut(s)	294, 409, 413, 483, 1053, 2201, 2205
BstDSI	CCRYGG	1 cut(s)	795
BstF5I	GGATG	6 cut(s)	57, 759, 879, 883, 1491, 2653
BstFNI	CGCG	1 cut(s)	318
BstH2I	RGCGCY	1 cut(s)	281
BstHHI	GCGC	2 cut(s)	280, 1237
BstMAI	GTCTC	9 cut(s)	110, 177, 357, 468, 825, 1460, 1468, 2180, 2603
BstNI	CCWGG	7 cut(s)	360, 1326, 1776, 2141, 2243, 2295, 2494
BstNSI	RCATGY	6 cut(s)	485, 516, 608, 1209, 1501, 2621
BstSCI	CCNGG	9 cut(s)	287, 358, 468, 1324, 1774, 2139, 2241, 2293, 2492
BstSFI	CTRYAG	2 cut(s)	273, 886
BstSLI	GKGCMC	2 cut(s)	615, 1183
BstSNI	TACGTA	1 cut(s)	1871
BstUI	CGCG	1 cut(s)	318
BstV1I	GCAGC	9 cut(s)	259, 281, 287, 356, 565, 979, 1046, 1726, 2551
BstV2I	GAAGAC	4 cut(s)	1262, 1343, 2291, 2614
BstX2I	RGATCY	2 cut(s)	424, 1411
BstYI	RGATCY	2 cut(s)	424, 1411
BstZ17I	GTATAC	1 cut(s)	499
BsuI	GTATCC	1 cut(s)	1512
BsuRI	GGCC	4 cut(s)	287, 800, 2199, 2492
BtgI	CCRYGG	1 cut(s)	795
BtgZI	GCGATG	2 cut(s)	333, 529
BtrI	CACGTC	1 cut(s)	2681
BtsCI	GGATG	6 cut(s)	57, 759, 879, 883, 1491, 2653
BtsI	GCAGTG	2 cut(s)	895, 1860
BtsIMutI	CAGTG	7 cut(s)	895, 933, 1390, 1860, 2032, 2189, 2488
BveI	ACCTGC	2 cut(s)	1312, 1696
Cac8I	GCNNGC	7 cut(s)	294, 409, 413, 483, 1053, 2201, 2205
CaiI	CAGNNNCTG	3 cut(s)	1739, 1775, 2009
CfoI	GCGC	2 cut(s)	280, 1237
Cfr10I	RCCGGY	2 cut(s)	485, 1664
Cfr13I	GGNCC	4 cut(s)	286, 1331, 1933, 2490
CseI	GACGC	1 cut(s)	119
CsiI	ACCWGGT	1 cut(s)	2293
Csp6I	GTAC	8 cut(s)	93, 557, 1301, 1763, 1962, 2218, 2504, 2586
CspAI	ACCGGT	1 cut(s)	1664
CviQI	GTAC	8 cut(s)	93, 557, 1301, 1763, 1962, 2218, 2504, 2586
DraIII	CACNNNGTG	2 cut(s)	1168, 2483
DrdI	GACNNNNNNGTC	2 cut(s)	2618, 2765
DseDI	GACNNNNNNGTC	2 cut(s)	2618, 2765
EaeI	YGGCCR	2 cut(s)	798, 2197
Eam1104I	CTCTTC	1 cut(s)	1396
EarI	CTCTTC	1 cut(s)	1396
Ecl136II	GAGCTC	1 cut(s)	1406
Eco105I	TACGTA	1 cut(s)	1871
Eco130I	CCWWGG	3 cut(s)	306, 795, 1334
Eco24I	GRGCYC	2 cut(s)	1408, 1493
Eco31I	GGTCTC	2 cut(s)	110, 2603
Eco47I	GGWCC	2 cut(s)	1331, 1933
Eco47III	AGCGCT	1 cut(s)	279
Eco53kI	GAGCTC	1 cut(s)	1406
Eco57I	CTGAAG	2 cut(s)	5, 2685
EcoICRI	GAGCTC	1 cut(s)	1406
EcoO109I	RGGNCCY	1 cut(s)	2490
EcoRII	CCWGG	7 cut(s)	358, 1324, 1774, 2139, 2241, 2293, 2492
EcoT14I	CCWWGG	3 cut(s)	306, 795, 1334
EcoT38I	GRGCYC	2 cut(s)	1408, 1493
ErhI	CCWWGG	3 cut(s)	306, 795, 1334
Esp3I	CGTCTC	2 cut(s)	177, 1468
FalI	AAGNNNNNCTT	4 cut(s)	1523, 1555, 2506, 2538
FblI	GTMKAC	2 cut(s)	498, 1552
FokI	GGATG	6 cut(s)	44, 746, 886, 890, 1498, 2660
FriOI	GRGCYC	2 cut(s)	1408, 1493
FspBI	CTAG	7 cut(s)	209, 353, 695, 1142, 1409, 2135, 2204
GlaI	GCGC	2 cut(s)	279, 1236
GsaI	CCCAGC	2 cut(s)	873, 2398
GsuI	CTGGAG	7 cut(s)	71, 155, 381, 1986, 2251, 2427, 2515
HaeII	RGCGCY	1 cut(s)	281
HaeIII	GGCC	4 cut(s)	287, 800, 2199, 2492
HapII	CCGG	4 cut(s)	289, 470, 486, 1665
HgaI	GACGC	1 cut(s)	119
HhaI	GCGC	2 cut(s)	280, 1237
Hin1I	GRCGYC	1 cut(s)	2766
Hin6I	GCGC	2 cut(s)	278, 1235
HinP1I	GCGC	2 cut(s)	278, 1235
HincII	GTYRAC	1 cut(s)	924
HindII	GTYRAC	1 cut(s)	924
HindIII	AAGCTT	1 cut(s)	238
HinfI	GANTC	7 cut(s)	204, 461, 937, 974, 1058, 2171, 2732
HpaI	GTTAAC	1 cut(s)	924
HpaII	CCGG	4 cut(s)	289, 470, 486, 1665
HphI	GGTGA	6 cut(s)	500, 536, 784, 1535, 1679, 2518
Hpy188I	TCNGA	6 cut(s)	115, 621, 942, 1219, 1756, 1806
Hpy99I	CGWCG	2 cut(s)	2679, 2682
HpyAV	CCTTC	8 cut(s)	29, 485, 574, 616, 746, 1109, 2231, 2719
HpyCH4III	ACNGT	9 cut(s)	781, 928, 1243, 1322, 1385, 1961, 2015, 2344, 2785
HpyCH4IV	ACGT	5 cut(s)	186, 1264, 1870, 2680, 2766
HpySE526I	ACGT	5 cut(s)	186, 1264, 1870, 2680, 2766
Hsp92I	GRCGYC	1 cut(s)	2766
HspAI	GCGC	2 cut(s)	278, 1235
KspAI	GTTAAC	1 cut(s)	924
LguI	GCTCTTC	1 cut(s)	1396
LmnI	GCTCC	3 cut(s)	301, 2270, 2408
Lsp1109I	GCAGC	9 cut(s)	259, 281, 287, 356, 565, 979, 1046, 1726, 2551
MabI	ACCWGGT	1 cut(s)	2293
MaeI	CTAG	7 cut(s)	209, 353, 695, 1142, 1409, 2135, 2204
MaeII	ACGT	5 cut(s)	186, 1264, 1870, 2680, 2766
MaeIII	GTNAC	6 cut(s)	472, 790, 1208, 2060, 2071, 2257
MfeI	CAATTG	6 cut(s)	12, 83, 660, 765, 1038, 1455
MflI	RGATCY	2 cut(s)	424, 1411
MhlI	GDGCHC	5 cut(s)	615, 885, 1183, 1408, 1493
MlsI	TGGCCA	2 cut(s)	800, 2199
MluNI	TGGCCA	2 cut(s)	800, 2199
MlyI	GAGTC	4 cut(s)	470, 968, 2180, 2741
MmeI	TCCRAC	3 cut(s)	1136, 1636, 1997
Mox20I	TGGCCA	2 cut(s)	800, 2199
MroXI	GAANNNNTTC	1 cut(s)	2126
MscI	TGGCCA	2 cut(s)	800, 2199
MseI	TTAA	8 cut(s)	629, 908, 923, 1428, 1641, 1677, 1953, 1992
MslI	CAYNNNNRTG	4 cut(s)	62, 486, 1383, 1614
Msp20I	TGGCCA	2 cut(s)	800, 2199
MspA1I	CMGCKG	2 cut(s)	272, 347
MspI	CCGG	4 cut(s)	289, 470, 486, 1665
MspR9I	CCNGG	9 cut(s)	289, 360, 470, 1326, 1776, 2141, 2243, 2295, 2494
MunI	CAATTG	6 cut(s)	12, 83, 660, 765, 1038, 1455
Mva1269I	GAATGC	1 cut(s)	1582
MvaI	CCWGG	7 cut(s)	360, 1326, 1776, 2141, 2243, 2295, 2494
MvnI	CGCG	1 cut(s)	318
NciI	CCSGG	2 cut(s)	289, 470
NcoI	CCATGG	1 cut(s)	795
NheI	GCTAGC	1 cut(s)	2203
NlaIV	GGNNCC	1 cut(s)	1312
NmuCI	GTSAC	2 cut(s)	790, 2071
NspI	RCATGY	6 cut(s)	485, 516, 608, 1209, 1501, 2621
OliI	CACNNNNGTG	1 cut(s)	1383
PaeI	GCATGC	1 cut(s)	485
PaqCI	CACCTGC	1 cut(s)	1696
PciI	ACATGT	4 cut(s)	512, 604, 1497, 2617
PciSI	GCTCTTC	1 cut(s)	1396
PcsI	WCGNNNNNNNCGW	1 cut(s)	1270
PctI	GAATGC	1 cut(s)	1582
PdmI	GAANNNNTTC	1 cut(s)	2126
PfeI	GAWTC	3 cut(s)	204, 937, 1058
PflFI	GACNNNGTC	1 cut(s)	2295
PflMI	CCANNNNNTGG	1 cut(s)	2274
PfoI	TCCNGGA	1 cut(s)	358
PinAI	ACCGGT	1 cut(s)	1664
PleI	GAGTC	4 cut(s)	469, 968, 2179, 2740
PpsI	GAGTC	4 cut(s)	469, 968, 2179, 2740
Ppu21I	YACGTR	1 cut(s)	1871
PscI	ACATGT	4 cut(s)	512, 604, 1497, 2617
PsiI	TTATAA	1 cut(s)	2432
Psp124BI	GAGCTC	1 cut(s)	1408
Psp6I	CCWGG	7 cut(s)	358, 1324, 1774, 2139, 2241, 2293, 2492
PspFI	CCCAGC	2 cut(s)	869, 2394
PspGI	CCWGG	7 cut(s)	358, 1324, 1774, 2139, 2241, 2293, 2492
PspN4I	GGNNCC	1 cut(s)	1312
PspPI	GGNCC	4 cut(s)	286, 1331, 1933, 2490
PstI	CTGCAG	2 cut(s)	277, 890
PstNI	CAGNNNCTG	3 cut(s)	1739, 1775, 2009
PsuI	RGATCY	2 cut(s)	424, 1411
PsyI	GACNNNGTC	1 cut(s)	2295
PvuII	CAGCTG	2 cut(s)	272, 347
RsaI	GTAC	8 cut(s)	94, 558, 1302, 1764, 1963, 2219, 2505, 2587
RsaNI	GTAC	8 cut(s)	93, 557, 1301, 1763, 1962, 2218, 2504, 2586
RseI	CAYNNNNRTG	4 cut(s)	62, 486, 1383, 1614
SacI	GAGCTC	1 cut(s)	1408
SapI	GCTCTTC	1 cut(s)	1396
SaqAI	TTAA	8 cut(s)	629, 908, 923, 1428, 1641, 1677, 1953, 1992
Sau96I	GGNCC	4 cut(s)	286, 1331, 1933, 2490
ScaI	AGTACT	1 cut(s)	1764
SchI	GAGTC	4 cut(s)	470, 968, 2180, 2741
ScrFI	CCNGG	9 cut(s)	289, 360, 470, 1326, 1776, 2141, 2243, 2295, 2494
SduI	GDGCHC	5 cut(s)	615, 885, 1183, 1408, 1493
SexAI	ACCWGGT	1 cut(s)	2293
SfcI	CTRYAG	2 cut(s)	273, 886
SinI	GGWCC	2 cut(s)	1331, 1933
SmiMI	CAYNNNNRTG	4 cut(s)	62, 486, 1383, 1614
SnaBI	TACGTA	1 cut(s)	1871
SphI	GCATGC	1 cut(s)	485
SsiI	CCGC	1 cut(s)	756
SspI	AATATT	1 cut(s)	2034
SspMI	CTAG	7 cut(s)	209, 353, 695, 1142, 1409, 2135, 2204
SstI	GAGCTC	1 cut(s)	1408
StyD4I	CCNGG	9 cut(s)	287, 358, 468, 1324, 1774, 2139, 2241, 2293, 2492
StyI	CCWWGG	3 cut(s)	306, 795, 1334
TaaI	ACNGT	9 cut(s)	781, 928, 1243, 1322, 1385, 1961, 2015, 2344, 2785
TaiI	ACGT	5 cut(s)	189, 1267, 1873, 2683, 2769
TaqI	TCGA	4 cut(s)	246, 438, 1273, 2174
TatI	WGTACW	3 cut(s)	92, 556, 1762
TauI	GCSGC	1 cut(s)	759
TfiI	GAWTC	3 cut(s)	204, 937, 1058
Tru1I	TTAA	8 cut(s)	629, 908, 923, 1428, 1641, 1677, 1953, 1992
Tru9I	TTAA	8 cut(s)	629, 908, 923, 1428, 1641, 1677, 1953, 1992
TscAI	CASTG	7 cut(s)	895, 933, 1390, 1860, 2032, 2196, 2488
TseFI	GTSAC	2 cut(s)	790, 2071
TseI	GCWGC	9 cut(s)	269, 272, 275, 344, 578, 992, 1034, 1739, 2564
Tsp45I	GTSAC	2 cut(s)	790, 2071
TspDTI	ATGAA	7 cut(s)	1503, 1578, 1847, 2272, 2308, 2372, 2582
TspRI	CASTG	7 cut(s)	895, 933, 1390, 1860, 2032, 2196, 2488
Tth111I	GACNNNGTC	1 cut(s)	2295
Van91I	CCANNNNNTGG	1 cut(s)	2274
VneI	GTGCAC	1 cut(s)	1179
VpaK11BI	GGWCC	2 cut(s)	1331, 1933
XapI	RAATTY	4 cut(s)	1253, 2369, 2379, 2706
XbaI	TCTAGA	2 cut(s)	1408, 2134
XceI	RCATGY	6 cut(s)	485, 516, 608, 1209, 1501, 2621
XcmI	CCANNNNNNNNNTGG	2 cut(s)	862, 2540
XmiI	GTMKAC	2 cut(s)	498, 1552
XmnI	GAANNNNTTC	1 cut(s)	2126
XspI	CTAG	7 cut(s)	209, 353, 695, 1142, 1409, 2135, 2204
ZraI	GACGTC	1 cut(s)	2767
ZrmI	AGTACT	1 cut(s)	1764

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.