Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002698

Physical Location & Seq

Genomic Coordinates Forward (+)

48618589 .. 48625981

Gene Structure 7393 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003448

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2889 bp

ATGGATACATCAATTGCCCTTCAGCCTATTACGCAGGAAGCTGTTGATCTGGAGAACATCCCTGTGGAGGAAGTTTTTGAACAATTGAAATGTACAGCAGCGGGTCTCAATTCTGAGGAGGTGCAAAAGCGTCTGGAGGTTTTTGGTTTCAACAAACTTCAAGAGAAAAAGGAAAGTAAAATACTTAACTTTCTGGGGTTTATGTGGAACCCTTTGTCTTGGGTTATGGAAGCAGCTGCACTAATGTCAATAACTCTCGCATATGGAGGCGGAAAAAAAAGAGACGTTGAGGATTTCTGTGGAATCCTAGCACTACTCCTTATCAATTCAACAATAAGCTTTATCGAGGAAAACAATGCTGGAAATGCAGCTGCAGCGCTTATGGCCCGGCTTGCTCCAAAAGCCAAGGTTTTACGCGATGGGAAATGGAATGAGGAAGATGCAGCTGTGTTGGTTCCTGGAGACATTATTAGTATCAAGTTAGGAGATATAATTCCTGCTGATGCTCGCCTGCTTCAAGGAGATCCTTTGAAGATCGACCAGTCTGCTCTGACAGGAGAGTCTCTCCCGGTAACTAAGCATGCCGGTGAAGGAGTATACTCTGGCTCAACATGTAAACAAGGTGAAATTGAAGCAGTTGTTATCGCAACAGGAGTACATACCTTCTTTGGAAAAGCTGCTCATCTTGTGGAAAACACAACACATGTTGGGCACTTTCAGAAGGTCTTAACAGCCATTGGAAACTTTTGCATCTGCTCAATTGCCATAGGTATTGTGATTGAACTAATTGTCCTAGTTCGTCAAGGAAAGAGTTATCGTTCTAAAATAGATAACCTTCTTGTACTACTTATTGGCGGCATCCCAATTGCTATGCCAACTGTTCTTTCTGTCACCATGGCCATTGGTTCTCATCGTTTGTCTCAGCAGGGTGCAATCACAAAGAGAATGACAGCCATTGAAGAAATGGCTGGGATGGATGTGCTCTGCAGTGATAAAACAGGAACTTTAACTCTCAACAAGTTAACAGTGGACAAGAATCTGATAGAGGTGTTTGCTAAAGATGTCGAAAAAGACTCGGTTGTATTGATGGCTGCAAGAGCTTCAAGGTTGGAAAATCAAGATGCCATTGATGCAGCAATTGTTGCAATGCTGGCTGATTCTAAGGAGGCACGAGCGGGAATTACAGAAGTTCACTTCCTTCCTTTCAATCCAACTGATAAGAGGACAGCACTTACATACCTAGACAAAGCTGGTAAAATGCACAGAGTGAGCAAAGGTGCACCAGAACAGATACTCAATTTGGCATGTAACAAATCAGATATTCAAAAGAGGGCACACACAGTAATTGACAAATTTGCAGAACGTGGTCTTCGATCCCTTGCTGTTGCACGACAGGAAGTACCTGCTGGAACCAAAGACAGTCCTGGTGGACCTTGGGAGTTTGTTGGTCTTCTACCTTTGTTTGATCCACCACGTCACGACAGTGCTGAGACTATTAGAAGAGCTCTAGATCTTGGTGTGAGTGTTAAGATGATTACAGGCGATCAACTAGCAATTGCCAAAGAGACAGGGAGACGACTTGGGATGGGCTCAAACATGTATCCGTCATCTTCATTGCTTGGTGAAAATAAGAATGGCTTAGATGCAAGTCTACAAATTGATGAACTCATTGAGAATGCTGATGGTTTTGCTGGTGTCTTTCCAGAGCATAAATATGAGATCGTTCAGCGATTACAAATTAAGAAGCACATAGTTGGAATGACCGGTGATGGAGTTAATGATGCACCTGCCTTGAAGAAAGCAGACATAGGAATTGCTGTGGCAGATGCCACAGATGCTGCTCGTAGTGCGTCTGATATAGTACTGACAGAGCCTGGATTGAGTGTTATTATTAGTGCTGTTCTGACAAGCCGTGCAATCTTTCAAAGAATGAAGAACTACACAATATATGCAGTGTCCATAACAATACGTATTGTGCTTGGTTTCTTGTTGCTAACTGCATTCTGGCGATTTGATTTCCCTCCTTTTATGGTCCTCATCATAGCCATTCTTAATGACGGTACTATTATGACAATATCCAAAGACAAGGTTAAGCCATCTCCAGTTCCTGACAGTTGGAAGCTCAGTGAAATATTTGCAACTGGGATTGTCTTAGGGAGTTACCTTGCCGTGACCACAGCTATATTTTTCTATATCATTTACGAAACCCACTTCTTCCCGAAACACTTCCATCTAGACCTGGACTTCAAGAAACTCGCTGATCTCAATGAGTTGAGGTATACAGAGACACTGAATGGCCAGCTAGCATCTGTTGTGTACCTTCAAGTTAGCACCATCAGCCAGGCACTAATTTTTGTAACTCGCTCCAGAGGTTGGTCATTCATGGAAAGACCCGGTCTTCTTCTTGTTGGTGCTTTCATAGTTGCTCAAATGGTTGCAACAGTAATATCTGCTCAGGCAACATGGAAATTTGCTCAAATTCATAGTGTTGGCTGGGGTTGGACTGGAGCTGTATGGGTTTACAACATTATAATCTATATGCTTCTTGATCCTATCAAGTTTTTTGTGCGATATGCACTCAGTGGGAGGGCCTGGAGTTTGGTACTGAACAAAAGGACGGCTTTCACCACCCAAAAGGACTTTGGTAGGGAAGCTCGTGAGGCTGCTTGGGCAACAGAACAGCGTACACTTCATGGTCTCCCAACTATGGACAGAAATGTCTTCCATGAGAGGCACACATTCAGGGATGTTAGCATGATGGCTGAAGAAGCCCGACGACGTGCAGAGATTGCAAGACTAAGGGAAATCCACACTCTCAAAGGGAAGGTGGAGTCCTTTGCAAAGCTAAGGGGTTTGGACATTGACGTCAATCCACATTACACAGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

963

Amino Acids

105.75

Weight (kDa)

7.08

Isoelectric Point (pI)

37.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	19 - 83	8.2e-15	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	131 - 231	7.3e-31	P-type ATPase actuator domain
Hydrolase	PF00702	325 - 602	8.6e-15	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002698)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60330 AT3G60330 AT3G60330 AT3G60330
fragaria_vesca	FvH4_5g19790 FvH4_7g09980
malus_domestica	MD02G1216000.v1.1 MD03G1197700.v1.1 MD03G1197800.v1.1 MD04G1058300.v1.1
prunus_persica	Prupe.2G128800_v2.0.a1 Prupe.5G062200_v2.0.a1
pyrus_communis	pycom04g05210
rosa_chinensis	RchiOBHm_Chr1g0347581 RchiOBHm_Chr4g0412601 RchiOBHm_Chr7g0204571
rosa_laevigata	RLG00000003448 RLG00000028736 RLG00000030415
rosa_multiflora	Rmu_sc0001211.1_g000115 Rmu_sc0005397.1_g000001 Rmu_sc0007327.1_g000001 Rmu_sc0021384.1_g000002 Rmu_sc0024460.1_g000001 Rmu_sc0027638.1_g000006
rosa_roxburghii	Rroxscaffold_3G00252980 Rroxscaffold_4G00307640 Rroxscaffold_4G00307710
rosa_rugosa	Rorug01G0188000 Rorug07G0085200
rosa_samantha	Rh1DG200900 Rh7AG214600 Rh7BG212100 Rh7CG227700 Rh7DG222300
rosa_wichuraiana	Rw0G013650 Rw0G018200 Rw1G017220 Rw7G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2531
AarI	CACCTGC	1 cut(s)	1795
AasI	GACNNNNNNGTC	3 cut(s)	559, 2717, 2864
AatII	GACGTC	1 cut(s)	2868
Acc36I	ACCTGC	2 cut(s)	1411, 1795
AccB7I	CCANNNNNTGG	1 cut(s)	2373
AccBSI	CCGCTC	1 cut(s)	1175
AccI	GTMKAC	3 cut(s)	597, 1651, 2279
AccII	CGCG	1 cut(s)	417
AciI	CCGC	4 cut(s)	101, 270, 855, 1175
AclWI	GGATC	4 cut(s)	518, 1368, 1460, 2543
AcoI	YGGCCR	2 cut(s)	897, 2296
AcsI	RAATTY	3 cut(s)	1352, 2468, 2478
AcuI	CTGAAG	2 cut(s)	5, 2784
AcyI	GRCGYC	1 cut(s)	2865
AdeI	CACNNNGTG	2 cut(s)	1267, 2582
AfaI	GTAC	9 cut(s)	94, 657, 843, 1401, 1863, 2062, 2318, 2604, 2686
AfeI	AGCGCT	1 cut(s)	378
AfiI	CCNNNNNNNGG	3 cut(s)	67, 2373, 2707
AflIII	ACRYGT	3 cut(s)	611, 703, 1596
AgeI	ACCGGT	1 cut(s)	1763
AjiI	CACGTC	2 cut(s)	1475, 2780
AjnI	CCWGG	6 cut(s)	457, 1423, 1873, 2238, 2340, 2591
AjuI	GAANNNNNNNTTGG	2 cut(s)	868, 900
AleI	CACNNNNGTG	1 cut(s)	1482
AloI	GAACNNNNNNTCC	2 cut(s)	774, 806
Alw21I	GWGCWC	3 cut(s)	984, 1282, 1507
Alw44I	GTGCAC	1 cut(s)	1278
AlwI	GGATC	4 cut(s)	518, 1368, 1460, 2543
AlwNI	CAGNNNCTG	3 cut(s)	1838, 1874, 2108
Aor51HI	AGCGCT	1 cut(s)	378
AoxI	GGCC	4 cut(s)	384, 897, 2296, 2589
ApaLI	GTGCAC	1 cut(s)	1278
ApoI	RAATTY	3 cut(s)	1352, 2468, 2478
AsiGI	ACCGGT	1 cut(s)	1763
Asp700I	GAANNNNTTC	1 cut(s)	2225
AspLEI	GCGC	1 cut(s)	379
AspS9I	GGNCC	4 cut(s)	385, 1430, 2032, 2589
AsuC2I	CCSGG	3 cut(s)	388, 569, 2394
AsuHPI	GGTGA	6 cut(s)	599, 635, 883, 1634, 1778, 2617
AsuNHI	GCTAGC	1 cut(s)	2302
AvaII	GGWCC	2 cut(s)	1430, 2032
BaeGI	GKGCMC	3 cut(s)	714, 1282, 1336
BalI	TGGCCA	2 cut(s)	899, 2298
BanII	GRGCYC	2 cut(s)	1507, 1592
BauI	CACGAG	2 cut(s)	1170, 2655
BbsI	GAAGAC	4 cut(s)	1361, 1442, 2390, 2713
Bbv12I	GWGCWC	3 cut(s)	984, 1282, 1507
BceAI	ACGGC	3 cut(s)	1896, 2153, 2634
BcgI	CGANNNNNNTGC	2 cut(s)	527, 561
BciT130I	CCWGG	6 cut(s)	459, 1425, 1875, 2240, 2342, 2593
BciVI	GTATCC	1 cut(s)	1611
BcnI	CCSGG	3 cut(s)	388, 569, 2394
BfaI	CTAG	7 cut(s)	308, 794, 1241, 1508, 1550, 2234, 2303
BfmI	CTRYAG	2 cut(s)	372, 985
BfoI	RGCGCY	1 cut(s)	380
BfuAI	ACCTGC	2 cut(s)	1411, 1795
BfuI	GTATCC	1 cut(s)	1611
BglII	AGATCT	1 cut(s)	1510
BmcAI	AGTACT	1 cut(s)	1863
Bme1390I	CCNGG	9 cut(s)	388, 459, 569, 1425, 1875, 2240, 2342, 2394, 2593
Bme18I	GGWCC	2 cut(s)	1430, 2032
BmgBI	CACGTC	2 cut(s)	1475, 2780
BmgT120I	GGNCC	4 cut(s)	385, 1430, 2032, 2589
BmiI	GGNNCC	3 cut(s)	209, 456, 1411
BmrFI	CCNGG	9 cut(s)	388, 459, 569, 1425, 1875, 2240, 2342, 2394, 2593
BmrI	ACTGGG	1 cut(s)	2151
BmtI	GCTAGC	1 cut(s)	2306
BmuI	ACTGGG	1 cut(s)	2151
BpiI	GAAGAC	4 cut(s)	1361, 1442, 2390, 2713
BplI	GAGNNNNNCTC	2 cut(s)	549, 581
BpmI	CTGGAG	7 cut(s)	71, 155, 480, 2085, 2350, 2526, 2614
Bpu10I	CCTNAGC	2 cut(s)	2454, 2846
BpuMI	CCSGG	3 cut(s)	388, 569, 2394
BsaAI	YACGTR	1 cut(s)	1970
BsaHI	GRCGYC	1 cut(s)	2865
BsaI	GGTCTC	2 cut(s)	110, 2702
BsaJI	CCNNGG	3 cut(s)	405, 894, 1433
BsaWI	WCCGGW	1 cut(s)	1763
Bsc4I	CCNNNNNNNGG	3 cut(s)	67, 2373, 2707
Bse118I	RCCGGY	2 cut(s)	584, 1763
Bse1I	ACTGG	4 cut(s)	541, 2102, 2146, 2509
Bse3DI	GCAATG	2 cut(s)	1152, 1613
BseBI	CCWGG	6 cut(s)	459, 1425, 1875, 2240, 2342, 2593
BseDI	CCNNGG	3 cut(s)	405, 894, 1433
BseGI	GGATG	6 cut(s)	57, 858, 978, 982, 1590, 2752
BseLI	CCNNNNNNNGG	3 cut(s)	67, 2373, 2707
BseMI	GCAATG	2 cut(s)	1152, 1613
BseMII	CTCAG	6 cut(s)	105, 935, 1479, 2137, 2468, 2593
BseNI	ACTGG	4 cut(s)	541, 2102, 2146, 2509
BseRI	GAGGAG	1 cut(s)	131
BseSI	GKGCMC	3 cut(s)	714, 1282, 1336
BseYI	CCCAGC	2 cut(s)	968, 2493
BsgI	GTGCAG	2 cut(s)	222, 2802
Bsh1236I	CGCG	1 cut(s)	417
BshFI	GGCC	4 cut(s)	386, 899, 2298, 2591
BshTI	ACCGGT	1 cut(s)	1763
BsiHKAI	GWGCWC	3 cut(s)	984, 1282, 1507
BsiSI	CCGG	5 cut(s)	388, 569, 585, 1764, 2394
BslI	CCNNNNNNNGG	3 cut(s)	67, 2373, 2707
BsmBI	CGTCTC	2 cut(s)	276, 1567
BsmI	GAATGC	2 cut(s)	1681, 2000
BsnI	GGCC	4 cut(s)	386, 899, 2298, 2591
Bso31I	GGTCTC	2 cut(s)	110, 2702
Bsp1286I	GDGCHC	6 cut(s)	714, 984, 1282, 1336, 1507, 1592
Bsp1407I	TGTACA	1 cut(s)	92
Bsp19I	CCATGG	1 cut(s)	894
BspACI	CCGC	4 cut(s)	101, 270, 855, 1175
BspANI	GGCC	4 cut(s)	386, 899, 2298, 2591
BspCNI	CTCAG	6 cut(s)	106, 934, 1480, 2136, 2467, 2592
BspFNI	CGCG	1 cut(s)	417
BspLI	GGNNCC	3 cut(s)	209, 456, 1411
BspMAI	CTGCAG	2 cut(s)	376, 989
BspMI	ACCTGC	2 cut(s)	1411, 1795
BspOI	GCTAGC	1 cut(s)	2306
BspPI	GGATC	4 cut(s)	518, 1368, 1460, 2543
BspQI	GCTCTTC	1 cut(s)	1495
BspTNI	GGTCTC	2 cut(s)	110, 2702
BsrBI	CCGCTC	1 cut(s)	1175
BsrDI	GCAATG	2 cut(s)	1152, 1613
BsrFI	RCCGGY	2 cut(s)	584, 1763
BsrGI	TGTACA	1 cut(s)	92
BsrI	ACTGG	4 cut(s)	541, 2102, 2146, 2509
BssAI	RCCGGY	2 cut(s)	584, 1763
BssECI	CCNNGG	3 cut(s)	405, 894, 1433
BssNAI	GTATAC	2 cut(s)	598, 2280
BssNI	GRCGYC	1 cut(s)	2865
BssSI	CACGAG	2 cut(s)	1170, 2655
BssT1I	CCWWGG	3 cut(s)	405, 894, 1433
Bst1107I	GTATAC	2 cut(s)	598, 2280
Bst2BI	CACGAG	2 cut(s)	1170, 2655
Bst2UI	CCWGG	6 cut(s)	459, 1425, 1875, 2240, 2342, 2593
Bst4CI	ACNGT	9 cut(s)	880, 1027, 1342, 1421, 1484, 2060, 2114, 2443, 2884
Bst6I	CTCTTC	1 cut(s)	1495
BstACI	GRCGYC	1 cut(s)	2865
BstAPI	GCANNNNNTGC	2 cut(s)	1142, 2789
BstAUI	TGTACA	1 cut(s)	92
BstBAI	YACGTR	1 cut(s)	1970
BstC8I	GCNNGC	7 cut(s)	393, 508, 512, 582, 1152, 2300, 2304
BstDSI	CCRYGG	1 cut(s)	894
BstF5I	GGATG	6 cut(s)	57, 858, 978, 982, 1590, 2752
BstFNI	CGCG	1 cut(s)	417
BstH2I	RGCGCY	1 cut(s)	380
BstHHI	GCGC	1 cut(s)	379
BstNI	CCWGG	6 cut(s)	459, 1425, 1875, 2240, 2342, 2593
BstNSI	RCATGY	5 cut(s)	584, 615, 707, 1308, 1600
BstSCI	CCNGG	9 cut(s)	386, 457, 567, 1423, 1873, 2238, 2340, 2392, 2591
BstSFI	CTRYAG	2 cut(s)	372, 985
BstSLI	GKGCMC	3 cut(s)	714, 1282, 1336
BstSNI	TACGTA	1 cut(s)	1970
BstUI	CGCG	1 cut(s)	417
BstV2I	GAAGAC	4 cut(s)	1361, 1442, 2390, 2713
BstX2I	RGATCY	2 cut(s)	523, 1510
BstYI	RGATCY	2 cut(s)	523, 1510
BstZ17I	GTATAC	2 cut(s)	598, 2280
BsuI	GTATCC	1 cut(s)	1611
BsuRI	GGCC	4 cut(s)	386, 899, 2298, 2591
BtgI	CCRYGG	1 cut(s)	894
BtgZI	GCGATG	1 cut(s)	432
BtrI	CACGTC	2 cut(s)	1475, 2780
BtsCI	GGATG	6 cut(s)	57, 858, 978, 982, 1590, 2752
BtsI	GCAGTG	2 cut(s)	994, 1959
BtsIMutI	CAGTG	7 cut(s)	994, 1032, 1489, 1959, 2131, 2288, 2587
BveI	ACCTGC	2 cut(s)	1411, 1795
Cac8I	GCNNGC	7 cut(s)	393, 508, 512, 582, 1152, 2300, 2304
CaiI	CAGNNNCTG	3 cut(s)	1838, 1874, 2108
CfoI	GCGC	1 cut(s)	379
Cfr10I	RCCGGY	2 cut(s)	584, 1763
Cfr13I	GGNCC	4 cut(s)	385, 1430, 2032, 2589
CseI	GACGC	2 cut(s)	119, 1839
Csp6I	GTAC	9 cut(s)	93, 656, 842, 1400, 1862, 2061, 2317, 2603, 2685
CspAI	ACCGGT	1 cut(s)	1763
CviQI	GTAC	9 cut(s)	93, 656, 842, 1400, 1862, 2061, 2317, 2603, 2685
DraIII	CACNNNGTG	2 cut(s)	1267, 2582
DrdI	GACNNNNNNGTC	3 cut(s)	559, 2717, 2864
DseDI	GACNNNNNNGTC	3 cut(s)	559, 2717, 2864
EaeI	YGGCCR	2 cut(s)	897, 2296
Eam1104I	CTCTTC	1 cut(s)	1495
EarI	CTCTTC	1 cut(s)	1495
EciI	GGCGGA	1 cut(s)	285
Ecl136II	GAGCTC	1 cut(s)	1505
Eco105I	TACGTA	1 cut(s)	1970
Eco130I	CCWWGG	3 cut(s)	405, 894, 1433
Eco24I	GRGCYC	2 cut(s)	1507, 1592
Eco31I	GGTCTC	2 cut(s)	110, 2702
Eco47I	GGWCC	2 cut(s)	1430, 2032
Eco47III	AGCGCT	1 cut(s)	378
Eco53kI	GAGCTC	1 cut(s)	1505
Eco57I	CTGAAG	2 cut(s)	5, 2784
EcoICRI	GAGCTC	1 cut(s)	1505
EcoO109I	RGGNCCY	1 cut(s)	2589
EcoRII	CCWGG	6 cut(s)	457, 1423, 1873, 2238, 2340, 2591
EcoT14I	CCWWGG	3 cut(s)	405, 894, 1433
EcoT38I	GRGCYC	2 cut(s)	1507, 1592
ErhI	CCWWGG	3 cut(s)	405, 894, 1433
Esp3I	CGTCTC	2 cut(s)	276, 1567
FalI	AAGNNNNNCTT	4 cut(s)	1622, 1654, 2605, 2637
FauI	CCCGC	2 cut(s)	94, 1168
FauNDI	CATATG	1 cut(s)	262
FblI	GTMKAC	3 cut(s)	597, 1651, 2279
FokI	GGATG	6 cut(s)	44, 845, 985, 989, 1597, 2759
FriOI	GRGCYC	2 cut(s)	1507, 1592
FspBI	CTAG	7 cut(s)	308, 794, 1241, 1508, 1550, 2234, 2303
GlaI	GCGC	1 cut(s)	378
GsaI	CCCAGC	2 cut(s)	972, 2497
GsuI	CTGGAG	7 cut(s)	71, 155, 480, 2085, 2350, 2526, 2614
HaeII	RGCGCY	1 cut(s)	380
HaeIII	GGCC	4 cut(s)	386, 899, 2298, 2591
HapII	CCGG	5 cut(s)	388, 569, 585, 1764, 2394
HgaI	GACGC	2 cut(s)	119, 1839
HhaI	GCGC	1 cut(s)	379
Hin1I	GRCGYC	1 cut(s)	2865
Hin6I	GCGC	1 cut(s)	377
HinP1I	GCGC	1 cut(s)	377
HincII	GTYRAC	1 cut(s)	1023
HindII	GTYRAC	1 cut(s)	1023
HindIII	AAGCTT	1 cut(s)	337
HinfI	GANTC	6 cut(s)	303, 560, 1036, 1073, 1157, 2831
HpaI	GTTAAC	1 cut(s)	1023
HpaII	CCGG	5 cut(s)	388, 569, 585, 1764, 2394
HphI	GGTGA	6 cut(s)	599, 635, 883, 1634, 1778, 2617
Hpy188I	TCNGA	7 cut(s)	115, 552, 720, 1041, 1318, 1855, 1905
Hpy99I	CGWCG	2 cut(s)	2778, 2781
HpyAV	CCTTC	8 cut(s)	29, 584, 673, 715, 845, 1208, 2330, 2818
HpyCH4III	ACNGT	9 cut(s)	880, 1027, 1342, 1421, 1484, 2060, 2114, 2443, 2884
HpyCH4IV	ACGT	6 cut(s)	285, 1363, 1474, 1969, 2779, 2865
HpySE526I	ACGT	6 cut(s)	285, 1363, 1474, 1969, 2779, 2865
Hsp92I	GRCGYC	1 cut(s)	2865
HspAI	GCGC	1 cut(s)	377
KspAI	GTTAAC	1 cut(s)	1023
LguI	GCTCTTC	1 cut(s)	1495
LmnI	GCTCC	3 cut(s)	400, 2369, 2507
MaeI	CTAG	7 cut(s)	308, 794, 1241, 1508, 1550, 2234, 2303
MaeII	ACGT	6 cut(s)	285, 1363, 1474, 1969, 2779, 2865
MaeIII	GTNAC	7 cut(s)	571, 889, 1307, 1475, 2159, 2170, 2356
MbiI	CCGCTC	1 cut(s)	1175
MfeI	CAATTG	6 cut(s)	12, 83, 759, 864, 1137, 1554
MflI	RGATCY	2 cut(s)	523, 1510
MhlI	GDGCHC	6 cut(s)	714, 984, 1282, 1336, 1507, 1592
MlsI	TGGCCA	2 cut(s)	899, 2298
MluNI	TGGCCA	2 cut(s)	899, 2298
MlyI	GAGTC	3 cut(s)	569, 1067, 2840
MmeI	TCCRAC	5 cut(s)	1089, 1235, 1735, 2096, 2480
Mox20I	TGGCCA	2 cut(s)	899, 2298
MroXI	GAANNNNTTC	1 cut(s)	2225
MscI	TGGCCA	2 cut(s)	899, 2298
MseI	TTAA	9 cut(s)	186, 728, 1007, 1022, 1527, 1740, 1776, 2052, 2091
MslI	CAYNNNNRTG	4 cut(s)	62, 585, 1482, 1713
Msp20I	TGGCCA	2 cut(s)	899, 2298
MspA1I	CMGCKG	4 cut(s)	101, 236, 371, 446
MspI	CCGG	5 cut(s)	388, 569, 585, 1764, 2394
MspR9I	CCNGG	9 cut(s)	388, 459, 569, 1425, 1875, 2240, 2342, 2394, 2593
MunI	CAATTG	6 cut(s)	12, 83, 759, 864, 1137, 1554
Mva1269I	GAATGC	2 cut(s)	1681, 2000
MvaI	CCWGG	6 cut(s)	459, 1425, 1875, 2240, 2342, 2593
MvnI	CGCG	1 cut(s)	417
NciI	CCSGG	3 cut(s)	388, 569, 2394
NcoI	CCATGG	1 cut(s)	894
NdeI	CATATG	1 cut(s)	262
NheI	GCTAGC	1 cut(s)	2302
NlaIV	GGNNCC	3 cut(s)	209, 456, 1411
NmuCI	GTSAC	3 cut(s)	889, 1475, 2170
NspI	RCATGY	5 cut(s)	584, 615, 707, 1308, 1600
OliI	CACNNNNGTG	1 cut(s)	1482
PaeI	GCATGC	1 cut(s)	584
PaqCI	CACCTGC	1 cut(s)	1795
PciI	ACATGT	3 cut(s)	611, 703, 1596
PciSI	GCTCTTC	1 cut(s)	1495
PcsI	WCGNNNNNNNCGW	1 cut(s)	1369
PctI	GAATGC	2 cut(s)	1681, 2000
PdmI	GAANNNNTTC	1 cut(s)	2225
PfeI	GAWTC	3 cut(s)	303, 1036, 1157
PflFI	GACNNNGTC	1 cut(s)	2394
PflMI	CCANNNNNTGG	1 cut(s)	2373
PfoI	TCCNGGA	1 cut(s)	457
PinAI	ACCGGT	1 cut(s)	1763
PleI	GAGTC	3 cut(s)	568, 1067, 2839
PpsI	GAGTC	3 cut(s)	568, 1067, 2839
Ppu21I	YACGTR	1 cut(s)	1970
PscI	ACATGT	3 cut(s)	611, 703, 1596
PsiI	TTATAA	1 cut(s)	2531
Psp124BI	GAGCTC	1 cut(s)	1507
Psp6I	CCWGG	6 cut(s)	457, 1423, 1873, 2238, 2340, 2591
PspFI	CCCAGC	2 cut(s)	968, 2493
PspGI	CCWGG	6 cut(s)	457, 1423, 1873, 2238, 2340, 2591
PspN4I	GGNNCC	3 cut(s)	209, 456, 1411
PspPI	GGNCC	4 cut(s)	385, 1430, 2032, 2589
PstI	CTGCAG	2 cut(s)	376, 989
PstNI	CAGNNNCTG	3 cut(s)	1838, 1874, 2108
PsuI	RGATCY	2 cut(s)	523, 1510
PsyI	GACNNNGTC	1 cut(s)	2394
PvuII	CAGCTG	3 cut(s)	236, 371, 446
RsaI	GTAC	9 cut(s)	94, 657, 843, 1401, 1863, 2062, 2318, 2604, 2686
RsaNI	GTAC	9 cut(s)	93, 656, 842, 1400, 1862, 2061, 2317, 2603, 2685
RseI	CAYNNNNRTG	4 cut(s)	62, 585, 1482, 1713
SacI	GAGCTC	1 cut(s)	1507
SapI	GCTCTTC	1 cut(s)	1495
SaqAI	TTAA	9 cut(s)	186, 728, 1007, 1022, 1527, 1740, 1776, 2052, 2091
Sau96I	GGNCC	4 cut(s)	385, 1430, 2032, 2589
ScaI	AGTACT	1 cut(s)	1863
SchI	GAGTC	3 cut(s)	569, 1067, 2840
ScrFI	CCNGG	9 cut(s)	388, 459, 569, 1425, 1875, 2240, 2342, 2394, 2593
SduI	GDGCHC	6 cut(s)	714, 984, 1282, 1336, 1507, 1592
SfcI	CTRYAG	2 cut(s)	372, 985
SinI	GGWCC	2 cut(s)	1430, 2032
SmiMI	CAYNNNNRTG	4 cut(s)	62, 585, 1482, 1713
SnaBI	TACGTA	1 cut(s)	1970
SphI	GCATGC	1 cut(s)	584
SsiI	CCGC	4 cut(s)	101, 270, 855, 1175
SspI	AATATT	1 cut(s)	2133
SspMI	CTAG	7 cut(s)	308, 794, 1241, 1508, 1550, 2234, 2303
SstI	GAGCTC	1 cut(s)	1507
StyD4I	CCNGG	9 cut(s)	386, 457, 567, 1423, 1873, 2238, 2340, 2392, 2591
StyI	CCWWGG	3 cut(s)	405, 894, 1433
TaaI	ACNGT	9 cut(s)	880, 1027, 1342, 1421, 1484, 2060, 2114, 2443, 2884
TaiI	ACGT	6 cut(s)	288, 1366, 1477, 1972, 2782, 2868
TaqI	TCGA	4 cut(s)	345, 537, 1065, 1372
TatI	WGTACW	4 cut(s)	92, 655, 841, 1861
TauI	GCSGC	1 cut(s)	858
TfiI	GAWTC	3 cut(s)	303, 1036, 1157
Tru1I	TTAA	9 cut(s)	186, 728, 1007, 1022, 1527, 1740, 1776, 2052, 2091
Tru9I	TTAA	9 cut(s)	186, 728, 1007, 1022, 1527, 1740, 1776, 2052, 2091
TscAI	CASTG	7 cut(s)	994, 1032, 1489, 1959, 2131, 2295, 2587
TseFI	GTSAC	3 cut(s)	889, 1475, 2170
Tsp45I	GTSAC	3 cut(s)	889, 1475, 2170
TspDTI	ATGAA	7 cut(s)	1602, 1677, 1946, 2371, 2407, 2471, 2681
TspGWI	ACGGA	1 cut(s)	1593
TspRI	CASTG	7 cut(s)	994, 1032, 1489, 1959, 2131, 2295, 2587
Tth111I	GACNNNGTC	1 cut(s)	2394
Van91I	CCANNNNNTGG	1 cut(s)	2373
VneI	GTGCAC	1 cut(s)	1278
VpaK11BI	GGWCC	2 cut(s)	1430, 2032
XapI	RAATTY	3 cut(s)	1352, 2468, 2478
XbaI	TCTAGA	2 cut(s)	1507, 2233
XceI	RCATGY	5 cut(s)	584, 615, 707, 1308, 1600
XcmI	CCANNNNNNNNNTGG	2 cut(s)	961, 2639
XmiI	GTMKAC	3 cut(s)	597, 1651, 2279
XmnI	GAANNNNTTC	1 cut(s)	2225
XspI	CTAG	7 cut(s)	308, 794, 1241, 1508, 1550, 2234, 2303
ZraI	GACGTC	1 cut(s)	2866
ZrmI	AGTACT	1 cut(s)	1863

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.