Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004395

Physical Location & Seq

Genomic Coordinates Reverse (-)

22812082 .. 22815216

Gene Structure 3135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G61650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGCCTCGGGAGATAATTACGCTTCAGGTTGGACAATGTGGGAATCAGATCGGTATGGAGTTCTGGAAACAGCTTTGCCTCGAGCACGGTATCAGTAAAGATGGTATTCTCGAGGACTTCGCTACTCAGGGAGGTGATAGAAAAGATGTGTTTTTTTACCAAGCGGATGACCAACACTATATCCCACGAGCTCTCCTCATTGATTTGGAGCCTAGAGTCATCAATGGTATTCAGAATGGTGATTACCGCAATCTCTATAATCACGAGAATATCTTCGTTGCTGACCATGGTGGTGGTGCTGGTAATAACTGGGCCAGTGGTTATCACCAGGGGAAGGGTGTTGAAGAGGAAATCATGGACATGATTGATCGAGAAGCTGATGGAAGTGACAGTCTTGAGGGTTTTGTTCTTTGCCACTCTATTGCTGGAGGCACTGGCTCAGGTATGGGATCTTATTTGTTGGAGACTTTGAATGATCGCTACAGCAAGAAGTTGGTTCAGACATACAGTGTCTTTCCCAATCAGATGGAAACGAGTGACGTTGTTGTCCAGCCCTACAACTCACTTTTGACGCTTAAGCGGCTTACATTAAATGCTGATTGTGTTGTTGTCCTTGACAACACTGCTTTGGGAAGGATTGCTGTGGAGCGTCTACATCTGACAAATCCTACCTTTGCTCAAACGAATTCTCTAGTGTCTACTGTGATGTCTGCTAGCACAACAACACTGCGTTATCCAGGATACATGAATAACGACCTCGTTGGCTTGCTTGCATCTTTGATCCCAACACCAAGGTGTCACTTCCTCATGACTGGATATACACCATTGACTGTTGAGCGCCAGGCAAATGTCATTCGTAAAACTACTGTGCTGGATGTTATGAGAAGACTTCTACAGACAAAAAATATCATGGTTTCGTCTTATGCTAGAAACAAAGAAGCTAGTCAGGCAAAGTATATATCAATATTGAATATAATTCAAGGAGAAGTCGATCCCACTCAGGTGCATGAGAGTTTGCAGAGGATACGAGAAAGAAAGCTTGTTAATTTCATTGAGTGGGGACCTGCAAGCATACAGGTTGCTCTTTCCAAGAAGTCCCCATATGTTCAAACTGCCCATAGGGTCAGTGGTCTTATGTTAGCAAGCCACACTAGTATCAGGCACCTATTCAGCAAATGTTTGAGCCAATATGATAAGTTGAGGAAGAAGCAAGCTTTTCTAGACAATTACCGGAAGTTTCCCATGTTTGCTGACAATGATCTTTCAGAGTTTGACGAATCAAGGGACATAATTGAGAGTTTGGTAGATGAATACAAGGCCTGTGAGTCTCCAGATTACATCAAATGGGGAATGGAGGACCCTGAACAGCTTATGACTGGTGAAGGCAATGCTTCAGGAGTTGTTGATCCGAAGTTGGCGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0000911 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005739 GO:0005856 GO:0005874 GO:0005886 GO:0005938 GO:0007049 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010016 GO:0010053 GO:0010054 GO:0010103 GO:0010374 GO:0012505 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0021700 GO:0022402 GO:0022622 GO:0030154 GO:0030312 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032506 GO:0032989 GO:0040007 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048827 GO:0048856 GO:0048869 GO:0050896 GO:0051179 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0060560 GO:0061640 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:1902410 GO:1903047 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko05165 map05165

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

474

Amino Acids

53.25

Weight (kDa)

5.61

Isoelectric Point (pI)

29.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	4 - 213	2.6e-61	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	264 - 393	3e-45	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004395)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61650 AT5G05620
fragaria_vesca	FvH4_7g15920
malus_domestica	MD01G1076100.v1.1 MD07G1145100.v1.1
prunus_persica	Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.2G100900_v2.0.a1 Prupe.2G100900_v2.0.a1 Prupe.8G052800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0358791
rosa_laevigata	RLG00000027923
rosa_multiflora	Rmu_sc0001063.1_g000016 Rmu_sc0002268.1_g000034 Rmu_sc0003660.1_g000005 Rmu_sc0004799.1_g000013 Rmu_sc0008757.1_g000022 Rmu_sc0031640.1_g000001
rosa_roxburghii	Rroxscaffold_4G00297630
rosa_rugosa	Rorug01G0265000 Rorug01G0265300
rosa_samantha	Rh1AG279400 Rh1BG245900 Rh1CG262700 Rh1CG263300 Rh1DG274300
rosa_wichuraiana	Rw1G024770 Rw1G024830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	545
Acc36I	ACCTGC	1 cut(s)	1072
AccB1I	GGYRCC	1 cut(s)	1161
AccI	GTMKAC	2 cut(s)	652, 698
AciI	CCGC	3 cut(s)	164, 247, 580
AclWI	GGATC	4 cut(s)	457, 775, 986, 1400
AcsI	RAATTY	1 cut(s)	685
AcuI	CTGAAG	2 cut(s)	8, 1377
AfiI	CCNNNNNNNGG	1 cut(s)	334
AflII	CTTAAG	1 cut(s)	575
AgsI	TTSAA	5 cut(s)	344, 472, 970, 980, 1109
AhlI	ACTAGT	1 cut(s)	1151
AjnI	CCWGG	3 cut(s)	327, 736, 840
AleI	CACNNNNGTG	2 cut(s)	793, 1001
AluBI	AGCT	7 cut(s)	73, 191, 377, 941, 1039, 1214, 1369
AluI	AGCT	7 cut(s)	73, 191, 377, 941, 1039, 1214, 1369
Alw21I	GWGCWC	2 cut(s)	87, 193
Alw26I	GTCTC	2 cut(s)	458, 1332
AlwI	GGATC	4 cut(s)	457, 775, 986, 1400
Ama87I	CYCGRG	3 cut(s)	6, 80, 110
AoxI	GGCC	2 cut(s)	312, 1317
ApoI	RAATTY	1 cut(s)	685
Asp700I	GAANNNNTTC	1 cut(s)	272
AspLEI	GCGC	1 cut(s)	840
AspS9I	GGNCC	3 cut(s)	312, 1061, 1357
AsuHPI	GGTGA	4 cut(s)	146, 251, 317, 1391
AsuNHI	GCTAGC	1 cut(s)	713
AvaI	CYCGRG	3 cut(s)	6, 80, 110
AvaII	GGWCC	2 cut(s)	1061, 1357
BaeI	ACNNNNGTAYC	2 cut(s)	1138, 1171
BanI	GGYRCC	1 cut(s)	1161
BanII	GRGCYC	1 cut(s)	193
BauI	CACGAG	2 cut(s)	186, 263
BbsI	GAAGAC	1 cut(s)	892
Bbv12I	GWGCWC	2 cut(s)	87, 193
BccI	CCATC	3 cut(s)	95, 374, 520
BciT130I	CCWGG	3 cut(s)	329, 738, 842
BciVI	GTATCC	2 cut(s)	734, 1017
BcoDI	GTCTC	2 cut(s)	458, 1332
BcuI	ACTAGT	1 cut(s)	1151
BfaI	CTAG	7 cut(s)	213, 692, 714, 927, 942, 1152, 1220
BfmI	CTRYAG	2 cut(s)	481, 893
BfoI	RGCGCY	1 cut(s)	841
BfrI	CTTAAG	1 cut(s)	575
BfuAI	ACCTGC	1 cut(s)	1072
BfuI	GTATCC	2 cut(s)	734, 1017
BisI	GCNGC	1 cut(s)	581
BlsI	GCNGC	1 cut(s)	582
Bme1390I	CCNGG	3 cut(s)	329, 738, 842
Bme18I	GGWCC	2 cut(s)	1061, 1357
BmeT110I	CYCGRG	3 cut(s)	6, 80, 110
BmgT120I	GGNCC	3 cut(s)	312, 1061, 1357
BmiI	GGNNCC	4 cut(s)	210, 1062, 1163, 1359
BmrFI	CCNGG	3 cut(s)	329, 738, 842
BmrI	ACTGGG	1 cut(s)	319
BmsI	GCATC	1 cut(s)	782
BmtI	GCTAGC	1 cut(s)	717
BmuI	ACTGGG	1 cut(s)	319
BpiI	GAAGAC	1 cut(s)	892
BplI	GAGNNNNNCTC	2 cut(s)	180, 212
BpmI	CTGGAG	2 cut(s)	447, 1314
Bpu10I	CCTNAGC	1 cut(s)	439
BpuEI	CTTGAG	1 cut(s)	416
BsaJI	CCNNGG	4 cut(s)	5, 286, 328, 791
BsaWI	WCCGGW	1 cut(s)	1230
BsaXI	ACNNNNNCTCC	2 cut(s)	177, 207
Bsc4I	CCNNNNNNNGG	1 cut(s)	334
Bse1I	ACTGG	5 cut(s)	314, 315, 439, 817, 1381
Bse3DI	GCAATG	1 cut(s)	1393
BseBI	CCWGG	3 cut(s)	329, 738, 842
BseDI	CCNNGG	4 cut(s)	5, 286, 328, 791
BseGI	GGATG	2 cut(s)	172, 880
BseLI	CCNNNNNNNGG	1 cut(s)	334
BseMI	GCAATG	1 cut(s)	1393
BseMII	CTCAG	3 cut(s)	140, 453, 1013
BseNI	ACTGG	5 cut(s)	314, 315, 439, 817, 1381
BseRI	GAGGAG	1 cut(s)	185
BshFI	GGCC	2 cut(s)	314, 1319
BshNI	GGYRCC	1 cut(s)	1161
BsiHKAI	GWGCWC	2 cut(s)	87, 193
BsiHKCI	CYCGRG	3 cut(s)	6, 80, 110
BsiSI	CCGG	1 cut(s)	1231
BslFI	GGGAC	3 cut(s)	1074, 1081, 1298
BslI	CCNNNNNNNGG	1 cut(s)	334
BsmAI	GTCTC	2 cut(s)	458, 1332
BsmFI	GGGAC	3 cut(s)	1074, 1081, 1298
BsnI	GGCC	2 cut(s)	314, 1319
BsoBI	CYCGRG	3 cut(s)	6, 80, 110
Bsp1286I	GDGCHC	2 cut(s)	87, 193
Bsp143I	GATC	8 cut(s)	48, 367, 449, 475, 780, 991, 1258, 1405
Bsp19I	CCATGG	1 cut(s)	286
BspACI	CCGC	3 cut(s)	164, 247, 580
BspANI	GGCC	2 cut(s)	314, 1319
BspCNI	CTCAG	3 cut(s)	139, 452, 1012
BspHI	TCATGA	1 cut(s)	807
BspLI	GGNNCC	4 cut(s)	210, 1062, 1163, 1359
BspMI	ACCTGC	1 cut(s)	1072
BspOI	GCTAGC	1 cut(s)	717
BspPI	GGATC	4 cut(s)	457, 775, 986, 1400
BspT107I	GGYRCC	1 cut(s)	1161
BspTI	CTTAAG	1 cut(s)	575
BsrDI	GCAATG	1 cut(s)	1393
BsrI	ACTGG	5 cut(s)	314, 315, 439, 817, 1381
BssECI	CCNNGG	4 cut(s)	5, 286, 328, 791
BssMI	GATC	8 cut(s)	48, 367, 449, 475, 780, 991, 1258, 1405
BssSI	CACGAG	2 cut(s)	186, 263
BssT1I	CCWWGG	2 cut(s)	286, 791
Bst2BI	CACGAG	2 cut(s)	186, 263
Bst2UI	CCWGG	3 cut(s)	329, 738, 842
Bst4CI	ACNGT	6 cut(s)	89, 392, 509, 703, 832, 868
Bst6I	CTCTTC	1 cut(s)	339
BstAFI	CTTAAG	1 cut(s)	575
BstC8I	GCNNGC	6 cut(s)	715, 767, 771, 1069, 1144, 1212
BstDEI	CTNAG	3 cut(s)	126, 439, 999
BstDSI	CCRYGG	1 cut(s)	286
BstF5I	GGATG	2 cut(s)	172, 880
BstH2I	RGCGCY	1 cut(s)	841
BstHHI	GCGC	1 cut(s)	840
BstKTI	GATC	8 cut(s)	51, 370, 452, 478, 783, 994, 1261, 1408
BstMAI	GTCTC	2 cut(s)	458, 1332
BstMBI	GATC	8 cut(s)	48, 367, 449, 475, 780, 991, 1258, 1405
BstMWI	GCNNNNNNNGC	2 cut(s)	580, 947
BstNI	CCWGG	3 cut(s)	329, 738, 842
BstSCI	CCNGG	3 cut(s)	327, 736, 840
BstSFI	CTRYAG	2 cut(s)	481, 893
BstV2I	GAAGAC	1 cut(s)	892
BstX2I	RGATCY	1 cut(s)	449
BstXI	CCANNNNNNTGG	2 cut(s)	293, 526
BstYI	RGATCY	1 cut(s)	449
BsuI	GTATCC	2 cut(s)	734, 1017
BsuRI	GGCC	2 cut(s)	314, 1319
BtgI	CCRYGG	1 cut(s)	286
BtsCI	GGATG	2 cut(s)	172, 880
BtsI	GCAGTG	2 cut(s)	621, 725
BtsIMutI	CAGTG	6 cut(s)	322, 432, 514, 621, 725, 1132
BveI	ACCTGC	1 cut(s)	1072
Cac8I	GCNNGC	6 cut(s)	715, 767, 771, 1069, 1144, 1212
CciI	TCATGA	1 cut(s)	807
CfoI	GCGC	1 cut(s)	840
Cfr13I	GGNCC	3 cut(s)	312, 1061, 1357
CseI	GACGC	2 cut(s)	580, 638
CviAII	CATG	8 cut(s)	287, 355, 361, 745, 808, 910, 1007, 1243
DdeI	CTNAG	3 cut(s)	126, 439, 999
DpnI	GATC	8 cut(s)	50, 369, 451, 477, 782, 993, 1260, 1407
DpnII	GATC	8 cut(s)	48, 367, 449, 475, 780, 991, 1258, 1405
DrdI	GACNNNNNNGTC	1 cut(s)	545
DseDI	GACNNNNNNGTC	1 cut(s)	545
Eam1104I	CTCTTC	1 cut(s)	339
EarI	CTCTTC	1 cut(s)	339
Ecl136II	GAGCTC	1 cut(s)	191
Eco130I	CCWWGG	2 cut(s)	286, 791
Eco147I	AGGCCT	1 cut(s)	1319
Eco24I	GRGCYC	1 cut(s)	193
Eco47I	GGWCC	2 cut(s)	1061, 1357
Eco53kI	GAGCTC	1 cut(s)	191
Eco57I	CTGAAG	2 cut(s)	8, 1377
Eco88I	CYCGRG	3 cut(s)	6, 80, 110
EcoICRI	GAGCTC	1 cut(s)	191
EcoO109I	RGGNCCY	2 cut(s)	1061, 1357
EcoRI	GAATTC	1 cut(s)	685
EcoRII	CCWGG	3 cut(s)	327, 736, 840
EcoT14I	CCWWGG	2 cut(s)	286, 791
EcoT38I	GRGCYC	1 cut(s)	193
ErhI	CCWWGG	2 cut(s)	286, 791
FaeI	CATG	8 cut(s)	290, 358, 364, 748, 811, 913, 1010, 1246
FaqI	GGGAC	3 cut(s)	1074, 1081, 1298
FatI	CATG	8 cut(s)	286, 354, 360, 744, 807, 909, 1006, 1242
FauNDI	CATATG	1 cut(s)	1102
FblI	GTMKAC	2 cut(s)	652, 698
Fnu4HI	GCNGC	1 cut(s)	581
FokI	GGATG	2 cut(s)	179, 887
FriOI	GRGCYC	1 cut(s)	193
Fsp4HI	GCNGC	1 cut(s)	581
FspBI	CTAG	7 cut(s)	213, 692, 714, 927, 942, 1152, 1220
GlaI	GCGC	1 cut(s)	839
GluI	GCNGC	1 cut(s)	581
GsuI	CTGGAG	2 cut(s)	447, 1314
HaeII	RGCGCY	1 cut(s)	841
HaeIII	GGCC	2 cut(s)	314, 1319
HapII	CCGG	1 cut(s)	1231
HgaI	GACGC	2 cut(s)	580, 638
HhaI	GCGC	1 cut(s)	840
Hin1II	CATG	8 cut(s)	290, 358, 364, 748, 811, 913, 1010, 1246
Hin6I	GCGC	1 cut(s)	838
HinP1I	GCGC	1 cut(s)	838
HindIII	AAGCTT	2 cut(s)	1037, 1212
HinfI	GANTC	4 cut(s)	43, 216, 1277, 1325
HpaII	CCGG	1 cut(s)	1231
HphI	GGTGA	4 cut(s)	146, 251, 317, 1391
Hpy166II	GTNNAC	2 cut(s)	653, 699
Hpy188I	TCNGA	8 cut(s)	48, 234, 501, 525, 660, 1267, 1410, 1424
Hpy8I	GTNNAC	2 cut(s)	653, 699
HpyAV	CCTTC	3 cut(s)	328, 627, 1376
HpyCH4III	ACNGT	6 cut(s)	89, 392, 509, 703, 832, 868
HpyCH4IV	ACGT	1 cut(s)	540
HpyCH4V	TGCA	4 cut(s)	773, 1006, 1018, 1067
HpyF10VI	GCNNNNNNNGC	2 cut(s)	580, 947
HpyF3I	CTNAG	3 cut(s)	126, 439, 999
HpySE526I	ACGT	1 cut(s)	540
Hsp92II	CATG	8 cut(s)	290, 358, 364, 748, 811, 913, 1010, 1246
HspAI	GCGC	1 cut(s)	838
Kzo9I	GATC	8 cut(s)	48, 367, 449, 475, 780, 991, 1258, 1405
LmnI	GCTCC	2 cut(s)	208, 646
LweI	GCATC	1 cut(s)	782
MaeI	CTAG	7 cut(s)	213, 692, 714, 927, 942, 1152, 1220
MaeII	ACGT	1 cut(s)	540
MaeIII	GTNAC	3 cut(s)	386, 536, 797
MalI	GATC	8 cut(s)	50, 369, 451, 477, 782, 993, 1260, 1407
MboI	GATC	8 cut(s)	48, 367, 449, 475, 780, 991, 1258, 1405
MboII	GAAGA	4 cut(s)	265, 356, 897, 1216
MflI	RGATCY	1 cut(s)	449
MhlI	GDGCHC	2 cut(s)	87, 193
MluCI	AATT	6 cut(s)	15, 685, 975, 1045, 1225, 1290
MlyI	GAGTC	2 cut(s)	225, 1334
MmeI	TCCRAC	2 cut(s)	10, 441
MroXI	GAANNNNTTC	1 cut(s)	272
MseI	TTAA	3 cut(s)	576, 590, 1044
MslI	CAYNNNNRTG	4 cut(s)	291, 359, 793, 1001
MspCI	CTTAAG	1 cut(s)	575
MspI	CCGG	1 cut(s)	1231
MspR9I	CCNGG	3 cut(s)	329, 738, 842
MvaI	CCWGG	3 cut(s)	329, 738, 842
MwoI	GCNNNNNNNGC	2 cut(s)	580, 947
NcoI	CCATGG	1 cut(s)	286
NdeI	CATATG	1 cut(s)	1102
NdeII	GATC	8 cut(s)	48, 367, 449, 475, 780, 991, 1258, 1405
NheI	GCTAGC	1 cut(s)	713
NlaIII	CATG	8 cut(s)	290, 358, 364, 748, 811, 913, 1010, 1246
NlaIV	GGNNCC	4 cut(s)	210, 1062, 1163, 1359
NmuCI	GTSAC	3 cut(s)	386, 536, 797
OliI	CACNNNNGTG	2 cut(s)	793, 1001
PaeR7I	CTCGAG	2 cut(s)	80, 110
PagI	TCATGA	1 cut(s)	807
PceI	AGGCCT	1 cut(s)	1319
PdmI	GAANNNNTTC	1 cut(s)	272
PfeI	GAWTC	2 cut(s)	43, 1277
PfoI	TCCNGGA	1 cut(s)	736
PkrI	GCNGC	1 cut(s)	582
PleI	GAGTC	2 cut(s)	224, 1333
PpsI	GAGTC	2 cut(s)	224, 1333
PpuMI	RGGWCCY	2 cut(s)	1061, 1357
Psp124BI	GAGCTC	1 cut(s)	193
Psp5II	RGGWCCY	2 cut(s)	1061, 1357
Psp6I	CCWGG	3 cut(s)	327, 736, 840
PspGI	CCWGG	3 cut(s)	327, 736, 840
PspN4I	GGNNCC	4 cut(s)	210, 1062, 1163, 1359
PspPI	GGNCC	3 cut(s)	312, 1061, 1357
PspPPI	RGGWCCY	2 cut(s)	1061, 1357
PspXI	VCTCGAGB	1 cut(s)	80
PsuI	RGATCY	1 cut(s)	449
RseI	CAYNNNNRTG	4 cut(s)	291, 359, 793, 1001
SacI	GAGCTC	1 cut(s)	193
SaqAI	TTAA	3 cut(s)	576, 590, 1044
SatI	GCNGC	1 cut(s)	581
Sau3AI	GATC	8 cut(s)	48, 367, 449, 475, 780, 991, 1258, 1405
Sau96I	GGNCC	3 cut(s)	312, 1061, 1357
SchI	GAGTC	2 cut(s)	225, 1334
ScrFI	CCNGG	3 cut(s)	329, 738, 842
SduI	GDGCHC	2 cut(s)	87, 193
SfaNI	GCATC	1 cut(s)	782
SfcI	CTRYAG	2 cut(s)	481, 893
Sfr274I	CTCGAG	2 cut(s)	80, 110
SinI	GGWCC	2 cut(s)	1061, 1357
SlaI	CTCGAG	2 cut(s)	80, 110
SmiMI	CAYNNNNRTG	4 cut(s)	291, 359, 793, 1001
SmlI	CTYRAG	4 cut(s)	80, 110, 395, 575
SmoI	CTYRAG	4 cut(s)	80, 110, 395, 575
SpeI	ACTAGT	1 cut(s)	1151
Sse9I	AATT	6 cut(s)	15, 685, 975, 1045, 1225, 1290
SseBI	AGGCCT	1 cut(s)	1319
SsiI	CCGC	3 cut(s)	164, 247, 580
SspI	AATATT	1 cut(s)	966
SspMI	CTAG	7 cut(s)	213, 692, 714, 927, 942, 1152, 1220
SstI	GAGCTC	1 cut(s)	193
StuI	AGGCCT	1 cut(s)	1319
StyD4I	CCNGG	3 cut(s)	327, 736, 840
StyI	CCWWGG	2 cut(s)	286, 791
TaaI	ACNGT	6 cut(s)	89, 392, 509, 703, 832, 868
TaiI	ACGT	1 cut(s)	543
TaqI	TCGA	4 cut(s)	81, 111, 370, 990
TasI	AATT	6 cut(s)	15, 685, 975, 1045, 1225, 1290
TauI	GCSGC	1 cut(s)	583
TfiI	GAWTC	2 cut(s)	43, 1277
Tru1I	TTAA	3 cut(s)	576, 590, 1044
Tru9I	TTAA	3 cut(s)	576, 590, 1044
TscAI	CASTG	6 cut(s)	322, 439, 514, 628, 732, 1132
TseFI	GTSAC	3 cut(s)	386, 536, 797
Tsp45I	GTSAC	3 cut(s)	386, 536, 797
TspDTI	ATGAA	3 cut(s)	761, 1039, 1323
TspRI	CASTG	6 cut(s)	322, 439, 514, 628, 732, 1132
Vha464I	CTTAAG	1 cut(s)	575
VpaK11BI	GGWCC	2 cut(s)	1061, 1357
XapI	RAATTY	1 cut(s)	685
XbaI	TCTAGA	1 cut(s)	1219
XcmI	CCANNNNNNNNNTGG	2 cut(s)	422, 1124
XhoI	CTCGAG	2 cut(s)	80, 110
XmiI	GTMKAC	2 cut(s)	652, 698
XmnI	GAANNNNTTC	1 cut(s)	272
XspI	CTAG	7 cut(s)	213, 692, 714, 927, 942, 1152, 1220

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.