Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004395

Physical Location & Seq

Genomic Coordinates Reverse (-)

18202324 .. 18206643

Gene Structure 4320 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1076100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGCCTCGAGAGATCATCACGTTGCAGGTGGGACAATGCGGGAACCAGATCGGAATGGAGTTCTGGAAGCAGCTCTGCCTCGAGCACGGAATCAGCAAGGAAGGCATTCTCGAGGACTTTGCCACTCAGGGAGGTGACCGGAAAGATGTGTTTTTCTACCAAGCTGATGATCAGCATTATATACCACGAGCTTTGTTGATCGATCTGGAGCCCAGAGTGATTAATGGGATTCAAAACAGCGAATATCGAAATCTCTACAATCATGAAAACATCTTTGTTTCAGATCATGGTGGCGGTGCAGGAAACAATTGGGCCAGTGGATACGATCAGGGGAAGGGTGTTGAAGAGGCTATAATGGACATGATTGATAGAGAAGCGGATGGGAGTGATAGTCTTGAAGGATTTGTTCTATGCCATTCAATTGCTGGAGGAACAGGCTCAGGTATGGGTTCCTATCTGTTGGAGACATTGAATGATAGATACAGCAAAAAACTGGTGCAGACATACAGTGTCTTTCCTAACCAGATGGAAACAAGTGATGTGGTGGTCCAGCCGTATAACTCACTTTTGACGCTTAAGCGACTAACGCTTAATGCAGACTGTGTTGTGGTCCTTGACAACACTGCATTGAACAGAATTGCTGTTGAGCGCCTTCATCTAACAAATCCCACCTTTGCCCAAACAAATTCTTTGGTTTCTACTGTAATGTCTGCCAGTACAACCACTCTGAGATATCCAGGGTACATGAACAATGACTTGGTTGGTCTGCTTGCATCTTTAATTCCAACACCAAGATGCCATTTTCTAATGACAGGATATACACCGCTCACAGTTGAGCGCCAGGCTAATGTGATTCGCAAAACCACTGTACTTGATGTTATGAGAAGACTTCTGCAGACGAAAAATATCATGGTTTCCTCTTATGCTCGAACAAAAGAAGCCAGTCAAGCAAAATACATTTCAATATTAAACATCATCCAAGGAGAAGTGGACCCTACTCAGGTTCATGAAAGTTTGCAGAGGATACGTGAAAGAAAGCTTGTTAACTTTATTGAATGGGGACCCGCAAGCATTCAGGTTGCTTTGTCTAGAAAATCTCCATATGTGCAGACTGCCCATAGGGTCAGTGGTCTTATGCTGGCGAGCCATACTAGTATTCGCCACCTCTTCAGTAAGTGTTTGAGCCAGTACTCATTGTTACGGAAGAAGCAAGCCTTTCTTGACAACTATCGGAAGTTCCCAATGTTTGCTGAGAATGACCTTTCGGAGTTTGACGAATCAAGAGAAATATTGGAGAGTTTGGTTGATGAATATAAGGCTTGTGAGTCCCCAGATTACATCAAATGGGGAATGGAGGATCCAGACCAAGTTCTTACAGGAGAAGGCAATGCATCAGGAACGGTCGACCCAAAATTGGCAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0000911 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005739 GO:0005856 GO:0005874 GO:0005886 GO:0005938 GO:0007049 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009624 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010016 GO:0010053 GO:0010054 GO:0010103 GO:0010374 GO:0012505 GO:0015630 GO:0016020 GO:0016043 GO:0016049 GO:0021700 GO:0022402 GO:0022622 GO:0030154 GO:0030312 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032506 GO:0032989 GO:0040007 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048827 GO:0048856 GO:0048869 GO:0050896 GO:0051179 GO:0051301 GO:0051641 GO:0051704 GO:0051707 GO:0060560 GO:0061640 GO:0071695 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0099080 GO:0099081 GO:0099402 GO:0099512 GO:0099513 GO:0099568 GO:1902410 GO:1903047 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko05165 map05165

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

475

Amino Acids

53.22

Weight (kDa)

5.48

Isoelectric Point (pI)

27.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	4 - 214	2.7e-61	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	264 - 393	7.4e-45	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004395)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G61650 AT5G05620
fragaria_vesca	FvH4_7g15920
malus_domestica	MD01G1076100.v1.1 MD07G1145100.v1.1
prunus_persica	Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.1G559600_v2.0.a1 Prupe.2G100900_v2.0.a1 Prupe.2G100900_v2.0.a1 Prupe.8G052800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0358791
rosa_laevigata	RLG00000027923
rosa_multiflora	Rmu_sc0001063.1_g000016 Rmu_sc0002268.1_g000034 Rmu_sc0003660.1_g000005 Rmu_sc0004799.1_g000013 Rmu_sc0008757.1_g000022 Rmu_sc0031640.1_g000001
rosa_roxburghii	Rroxscaffold_4G00297630
rosa_rugosa	Rorug01G0265000 Rorug01G0265300
rosa_samantha	Rh1AG279400 Rh1BG245900 Rh1CG262700 Rh1CG263300 Rh1DG274300
rosa_wichuraiana	Rw1G024770 Rw1G024830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	16
Acc36I	ACCTGC	1 cut(s)	16
AccBSI	CCGCTC	1 cut(s)	826
AccI	GTMKAC	1 cut(s)	1404
AciI	CCGC	5 cut(s)	39, 294, 377, 824, 1065
AclWI	GGATC	2 cut(s)	1352, 1365
AcsI	RAATTY	1 cut(s)	685
AcuI	CTGAAG	1 cut(s)	1153
AfaI	GTAC	4 cut(s)	718, 743, 870, 1190
AfiI	CCNNNNNNNGG	2 cut(s)	1000, 1414
AflII	CTTAAG	1 cut(s)	575
AgsI	TTSAA	8 cut(s)	233, 344, 398, 420, 472, 631, 963, 1055
AhlI	ACTAGT	1 cut(s)	1151
AjnI	CCWGG	2 cut(s)	736, 840
AjuI	GAANNNNNNNTTGG	2 cut(s)	740, 772
AluBI	AGCT	4 cut(s)	73, 164, 191, 1039
AluI	AGCT	4 cut(s)	73, 164, 191, 1039
Alw21I	GWGCWC	1 cut(s)	87
Alw26I	GTCTC	1 cut(s)	458
AlwI	GGATC	2 cut(s)	1352, 1365
Ama87I	CYCGRG	3 cut(s)	6, 80, 110
AoxI	GGCC	1 cut(s)	312
ApeKI	GCWGC	1 cut(s)	70
ApoI	RAATTY	1 cut(s)	685
AseI	ATTAAT	1 cut(s)	222
Asp700I	GAANNNNTTC	1 cut(s)	105
AspLEI	GCGC	2 cut(s)	651, 840
AspS9I	GGNCC	5 cut(s)	312, 547, 610, 991, 1061
AsuHPI	GGTGA	1 cut(s)	146
AvaI	CYCGRG	3 cut(s)	6, 80, 110
AvaII	GGWCC	4 cut(s)	547, 610, 991, 1061
BamHI	GGATCC	1 cut(s)	1357
BanII	GRGCYC	1 cut(s)	213
BauI	CACGAG	1 cut(s)	186
BbsI	GAAGAC	1 cut(s)	892
Bbv12I	GWGCWC	1 cut(s)	87
BbvI	GCAGC	1 cut(s)	82
BccI	CCATC	2 cut(s)	374, 520
BceAI	ACGGC	1 cut(s)	538
BcgI	CGANNNNNNTGC	2 cut(s)	101, 135
BciT130I	CCWGG	2 cut(s)	738, 842
BciVI	GTATCC	2 cut(s)	314, 1017
BclI	TGATCA	1 cut(s)	169
BcoDI	GTCTC	1 cut(s)	458
BcuI	ACTAGT	1 cut(s)	1151
BfaI	CTAG	2 cut(s)	1089, 1152
BfmI	CTRYAG	1 cut(s)	893
BfoI	RGCGCY	2 cut(s)	652, 841
BfrI	CTTAAG	1 cut(s)	575
BfuAI	ACCTGC	1 cut(s)	16
BfuI	GTATCC	2 cut(s)	314, 1017
BisI	GCNGC	1 cut(s)	71
BlsI	GCNGC	1 cut(s)	72
BmcAI	AGTACT	1 cut(s)	1190
Bme1390I	CCNGG	2 cut(s)	738, 842
Bme18I	GGWCC	4 cut(s)	547, 610, 991, 1061
BmeT110I	CYCGRG	3 cut(s)	6, 80, 110
BmgT120I	GGNCC	5 cut(s)	312, 547, 610, 991, 1061
BmiI	GGNNCC	7 cut(s)	44, 210, 451, 993, 1062, 1063, 1359
BmrFI	CCNGG	2 cut(s)	738, 842
BmsI	GCATC	3 cut(s)	782, 785, 1400
BpiI	GAAGAC	1 cut(s)	892
BpmI	CTGGAG	2 cut(s)	227, 447
Bpu10I	CCTNAGC	1 cut(s)	439
Bsa29I	ATCGAT	1 cut(s)	201
BsaAI	YACGTR	1 cut(s)	1028
BsaJI	CCNNGG	2 cut(s)	737, 979
BsaWI	WCCGGW	1 cut(s)	138
BsaXI	ACNNNNNCTCC	2 cut(s)	376, 406
Bsc4I	CCNNNNNNNGG	2 cut(s)	1000, 1414
Bse1I	ACTGG	5 cut(s)	315, 498, 714, 942, 1186
Bse3DI	GCAATG	1 cut(s)	1393
BseBI	CCWGG	2 cut(s)	738, 842
BseCI	ATCGAT	1 cut(s)	201
BseDI	CCNNGG	2 cut(s)	737, 979
BseGI	GGATG	2 cut(s)	385, 975
BseLI	CCNNNNNNNGG	2 cut(s)	1000, 1414
BseMI	GCAATG	1 cut(s)	1393
BseMII	CTCAG	5 cut(s)	140, 453, 719, 1013, 1242
BseNI	ACTGG	5 cut(s)	315, 498, 714, 942, 1186
BseXI	GCAGC	1 cut(s)	82
BsgI	GTGCAG	3 cut(s)	318, 518, 1127
Bsh1285I	CGRYCG	1 cut(s)	1404
BshFI	GGCC	1 cut(s)	314
BshVI	ATCGAT	1 cut(s)	201
BsiEI	CGRYCG	1 cut(s)	1404
BsiHKAI	GWGCWC	1 cut(s)	87
BsiHKCI	CYCGRG	3 cut(s)	6, 80, 110
BsiSI	CCGG	1 cut(s)	139
BslFI	GGGAC	3 cut(s)	45, 1074, 1312
BslI	CCNNNNNNNGG	2 cut(s)	1000, 1414
BsmAI	GTCTC	1 cut(s)	458
BsmFI	GGGAC	3 cut(s)	45, 1074, 1312
BsmI	GAATGC	2 cut(s)	105, 1071
BsnI	GGCC	1 cut(s)	314
BsoBI	CYCGRG	3 cut(s)	6, 80, 110
Bsp1286I	GDGCHC	2 cut(s)	87, 213
Bsp143I	GATC	8 cut(s)	12, 48, 169, 198, 202, 283, 325, 1357
BspACI	CCGC	5 cut(s)	39, 294, 377, 824, 1065
BspANI	GGCC	1 cut(s)	314
BspCNI	CTCAG	5 cut(s)	139, 452, 720, 1012, 1243
BspDI	ATCGAT	1 cut(s)	201
BspHI	TCATGA	2 cut(s)	262, 1006
BspLI	GGNNCC	7 cut(s)	44, 210, 451, 993, 1062, 1063, 1359
BspMAI	CTGCAG	1 cut(s)	897
BspMI	ACCTGC	1 cut(s)	16
BspPI	GGATC	2 cut(s)	1352, 1365
BspTI	CTTAAG	1 cut(s)	575
BsrBI	CCGCTC	1 cut(s)	826
BsrDI	GCAATG	1 cut(s)	1393
BsrI	ACTGG	5 cut(s)	315, 498, 714, 942, 1186
BssECI	CCNNGG	2 cut(s)	737, 979
BssMI	GATC	8 cut(s)	12, 48, 169, 198, 202, 283, 325, 1357
BssSI	CACGAG	1 cut(s)	186
BssT1I	CCWWGG	1 cut(s)	979
Bst2BI	CACGAG	1 cut(s)	186
Bst2UI	CCWGG	2 cut(s)	738, 842
Bst4CI	ACNGT	6 cut(s)	509, 602, 703, 832, 868, 1402
Bst6I	CTCTTC	2 cut(s)	339, 1172
BstAFI	CTTAAG	1 cut(s)	575
BstBAI	YACGTR	1 cut(s)	1028
BstC8I	GCNNGC	5 cut(s)	771, 1069, 1140, 1144, 1212
BstDEI	CTNAG	5 cut(s)	126, 439, 728, 999, 1251
BstEII	GGTNACC	1 cut(s)	134
BstF5I	GGATG	2 cut(s)	385, 975
BstH2I	RGCGCY	2 cut(s)	652, 841
BstHHI	GCGC	2 cut(s)	651, 840
BstKTI	GATC	8 cut(s)	15, 51, 172, 201, 205, 286, 328, 1360
BstMAI	GTCTC	1 cut(s)	458
BstMBI	GATC	8 cut(s)	12, 48, 169, 198, 202, 283, 325, 1357
BstMCI	CGRYCG	1 cut(s)	1404
BstMWI	GCNNNNNNNGC	3 cut(s)	102, 586, 947
BstNI	CCWGG	2 cut(s)	738, 842
BstPI	GGTNACC	1 cut(s)	134
BstSCI	CCNGG	2 cut(s)	736, 840
BstSFI	CTRYAG	1 cut(s)	893
BstV1I	GCAGC	1 cut(s)	82
BstV2I	GAAGAC	1 cut(s)	892
BstX2I	RGATCY	1 cut(s)	1357
BstYI	RGATCY	1 cut(s)	1357
Bsu15I	ATCGAT	1 cut(s)	201
BsuI	GTATCC	2 cut(s)	314, 1017
BsuRI	GGCC	1 cut(s)	314
BsuTUI	ATCGAT	1 cut(s)	201
BtsCI	GGATG	2 cut(s)	385, 975
BtsI	GCAGTG	1 cut(s)	621
BtsIMutI	CAGTG	5 cut(s)	322, 514, 621, 864, 1132
BveI	ACCTGC	1 cut(s)	16
Cac8I	GCNNGC	5 cut(s)	771, 1069, 1140, 1144, 1212
CciI	TCATGA	2 cut(s)	262, 1006
CfoI	GCGC	2 cut(s)	651, 840
Cfr13I	GGNCC	5 cut(s)	312, 547, 610, 991, 1061
ClaI	ATCGAT	1 cut(s)	201
CseI	GACGC	1 cut(s)	580
Csp6I	GTAC	4 cut(s)	717, 742, 869, 1189
CspCI	CAANNNNNGTGG	6 cut(s)	174, 209, 522, 557, 853, 888
CviAII	CATG	6 cut(s)	263, 287, 361, 745, 910, 1007
CviQI	GTAC	4 cut(s)	717, 742, 869, 1189
DdeI	CTNAG	5 cut(s)	126, 439, 728, 999, 1251
DpnI	GATC	8 cut(s)	14, 50, 171, 200, 204, 285, 327, 1359
DpnII	GATC	8 cut(s)	12, 48, 169, 198, 202, 283, 325, 1357
Eam1104I	CTCTTC	2 cut(s)	339, 1172
EarI	CTCTTC	2 cut(s)	339, 1172
Eco130I	CCWWGG	1 cut(s)	979
Eco24I	GRGCYC	1 cut(s)	213
Eco32I	GATATC	1 cut(s)	734
Eco47I	GGWCC	4 cut(s)	547, 610, 991, 1061
Eco57I	CTGAAG	1 cut(s)	1153
Eco88I	CYCGRG	3 cut(s)	6, 80, 110
Eco91I	GGTNACC	1 cut(s)	134
EcoO109I	RGGNCCY	1 cut(s)	1061
EcoO65I	GGTNACC	1 cut(s)	134
EcoRII	CCWGG	2 cut(s)	736, 840
EcoRV	GATATC	1 cut(s)	734
EcoT14I	CCWWGG	1 cut(s)	979
EcoT22I	ATGCAT	1 cut(s)	1393
EcoT38I	GRGCYC	1 cut(s)	213
ErhI	CCWWGG	1 cut(s)	979
FaeI	CATG	6 cut(s)	266, 290, 364, 748, 913, 1010
FalI	AAGNNNNNCTT	4 cut(s)	1199, 1231, 1203, 1235
FaqI	GGGAC	3 cut(s)	45, 1074, 1312
FatI	CATG	6 cut(s)	262, 286, 360, 744, 909, 1006
FauI	CCCGC	2 cut(s)	32, 1072
FauNDI	CATATG	1 cut(s)	1102
FbaI	TGATCA	1 cut(s)	169
FblI	GTMKAC	1 cut(s)	1404
Fnu4HI	GCNGC	1 cut(s)	71
FokI	GGATG	2 cut(s)	392, 962
FriOI	GRGCYC	1 cut(s)	213
Fsp4HI	GCNGC	1 cut(s)	71
FspBI	CTAG	2 cut(s)	1089, 1152
GlaI	GCGC	2 cut(s)	650, 839
GluI	GCNGC	1 cut(s)	71
GsuI	CTGGAG	2 cut(s)	227, 447
HaeII	RGCGCY	2 cut(s)	652, 841
HaeIII	GGCC	1 cut(s)	314
HapII	CCGG	1 cut(s)	139
HgaI	GACGC	1 cut(s)	580
HhaI	GCGC	2 cut(s)	651, 840
Hin1II	CATG	6 cut(s)	266, 290, 364, 748, 913, 1010
Hin6I	GCGC	2 cut(s)	649, 838
HinP1I	GCGC	2 cut(s)	649, 838
HincII	GTYRAC	2 cut(s)	1045, 1405
HindII	GTYRAC	2 cut(s)	1045, 1405
HindIII	AAGCTT	1 cut(s)	1037
HinfI	GANTC	5 cut(s)	90, 229, 853, 1277, 1325
HpaI	GTTAAC	1 cut(s)	1045
HpaII	CCGG	1 cut(s)	139
HphI	GGTGA	1 cut(s)	146
Hpy166II	GTNNAC	3 cut(s)	991, 1045, 1405
Hpy188I	TCNGA	5 cut(s)	53, 283, 729, 1233, 1267
Hpy8I	GTNNAC	3 cut(s)	991, 1045, 1405
HpyAV	CCTTC	5 cut(s)	95, 328, 392, 662, 1376
HpyCH4III	ACNGT	6 cut(s)	509, 602, 703, 832, 868, 1402
HpyCH4IV	ACGT	2 cut(s)	20, 1027
HpyF10VI	GCNNNNNNNGC	3 cut(s)	102, 586, 947
HpyF3I	CTNAG	5 cut(s)	126, 439, 728, 999, 1251
HpySE526I	ACGT	2 cut(s)	20, 1027
Hsp92II	CATG	6 cut(s)	266, 290, 364, 748, 913, 1010
HspAI	GCGC	2 cut(s)	649, 838
KflI	GGGWCCC	1 cut(s)	1061
Ksp22I	TGATCA	1 cut(s)	169
KspAI	GTTAAC	1 cut(s)	1045
Kzo9I	GATC	8 cut(s)	12, 48, 169, 198, 202, 283, 325, 1357
LmnI	GCTCC	1 cut(s)	208
Lsp1109I	GCAGC	1 cut(s)	82
LweI	GCATC	3 cut(s)	782, 785, 1400
MaeI	CTAG	2 cut(s)	1089, 1152
MaeII	ACGT	2 cut(s)	20, 1027
MaeIII	GTNAC	2 cut(s)	134, 1197
MalI	GATC	8 cut(s)	14, 50, 171, 200, 204, 285, 327, 1359
MbiI	CCGCTC	1 cut(s)	826
MboI	GATC	8 cut(s)	12, 48, 169, 198, 202, 283, 325, 1357
MboII	GAAGA	4 cut(s)	356, 897, 1159, 1216
MfeI	CAATTG	2 cut(s)	307, 420
MflI	RGATCY	1 cut(s)	1357
MhlI	GDGCHC	2 cut(s)	87, 213
MluCI	AATT	6 cut(s)	307, 420, 636, 685, 780, 1412
MlyI	GAGTC	1 cut(s)	1334
MmeI	TCCRAC	2 cut(s)	441, 809
Mph1103I	ATGCAT	1 cut(s)	1393
MroXI	GAANNNNTTC	1 cut(s)	105
MseI	TTAA	6 cut(s)	222, 576, 591, 779, 968, 1044
MslI	CAYNNNNRTG	1 cut(s)	793
MspCI	CTTAAG	1 cut(s)	575
MspI	CCGG	1 cut(s)	139
MspR9I	CCNGG	2 cut(s)	738, 842
MunI	CAATTG	2 cut(s)	307, 420
Mva1269I	GAATGC	2 cut(s)	105, 1071
MvaI	CCWGG	2 cut(s)	738, 842
MwoI	GCNNNNNNNGC	3 cut(s)	102, 586, 947
NdeI	CATATG	1 cut(s)	1102
NdeII	GATC	8 cut(s)	12, 48, 169, 198, 202, 283, 325, 1357
NlaIII	CATG	6 cut(s)	266, 290, 364, 748, 913, 1010
NlaIV	GGNNCC	7 cut(s)	44, 210, 451, 993, 1062, 1063, 1359
NmuCI	GTSAC	1 cut(s)	134
NsiI	ATGCAT	1 cut(s)	1393
PaeR7I	CTCGAG	3 cut(s)	6, 80, 110
PagI	TCATGA	2 cut(s)	262, 1006
PaqCI	CACCTGC	1 cut(s)	16
PctI	GAATGC	2 cut(s)	105, 1071
PdmI	GAANNNNTTC	1 cut(s)	105
PfeI	GAWTC	4 cut(s)	90, 229, 853, 1277
PkrI	GCNGC	1 cut(s)	72
PleI	GAGTC	1 cut(s)	1333
PpsI	GAGTC	1 cut(s)	1333
Ppu21I	YACGTR	1 cut(s)	1028
PpuMI	RGGWCCY	1 cut(s)	1061
PshBI	ATTAAT	1 cut(s)	222
Psp5II	RGGWCCY	1 cut(s)	1061
Psp6I	CCWGG	2 cut(s)	736, 840
PspEI	GGTNACC	1 cut(s)	134
PspGI	CCWGG	2 cut(s)	736, 840
PspN4I	GGNNCC	7 cut(s)	44, 210, 451, 993, 1062, 1063, 1359
PspPI	GGNCC	5 cut(s)	312, 547, 610, 991, 1061
PspPPI	RGGWCCY	1 cut(s)	1061
PspXI	VCTCGAGB	1 cut(s)	80
PstI	CTGCAG	1 cut(s)	897
PsuI	RGATCY	1 cut(s)	1357
RsaI	GTAC	4 cut(s)	718, 743, 870, 1190
RsaNI	GTAC	4 cut(s)	717, 742, 869, 1189
RseI	CAYNNNNRTG	1 cut(s)	793
SalI	GTCGAC	1 cut(s)	1403
SaqAI	TTAA	6 cut(s)	222, 576, 591, 779, 968, 1044
SatI	GCNGC	1 cut(s)	71
Sau3AI	GATC	8 cut(s)	12, 48, 169, 198, 202, 283, 325, 1357
Sau96I	GGNCC	5 cut(s)	312, 547, 610, 991, 1061
ScaI	AGTACT	1 cut(s)	1190
SchI	GAGTC	1 cut(s)	1334
ScrFI	CCNGG	2 cut(s)	738, 842
SduI	GDGCHC	2 cut(s)	87, 213
SfaNI	GCATC	3 cut(s)	782, 785, 1400
SfcI	CTRYAG	1 cut(s)	893
Sfr274I	CTCGAG	3 cut(s)	6, 80, 110
SinI	GGWCC	4 cut(s)	547, 610, 991, 1061
SlaI	CTCGAG	3 cut(s)	6, 80, 110
SmiMI	CAYNNNNRTG	1 cut(s)	793
SmlI	CTYRAG	4 cut(s)	6, 80, 110, 575
SmoI	CTYRAG	4 cut(s)	6, 80, 110, 575
SpeI	ACTAGT	1 cut(s)	1151
Sse9I	AATT	6 cut(s)	307, 420, 636, 685, 780, 1412
SsiI	CCGC	5 cut(s)	39, 294, 377, 824, 1065
SspI	AATATT	2 cut(s)	966, 1290
SspMI	CTAG	2 cut(s)	1089, 1152
StyD4I	CCNGG	2 cut(s)	736, 840
StyI	CCWWGG	1 cut(s)	979
TaaI	ACNGT	6 cut(s)	509, 602, 703, 832, 868, 1402
TaiI	ACGT	2 cut(s)	23, 1030
TaqI	TCGA	7 cut(s)	7, 81, 111, 201, 247, 928, 1404
TasI	AATT	6 cut(s)	307, 420, 636, 685, 780, 1412
TatI	WGTACW	3 cut(s)	716, 868, 1188
TfiI	GAWTC	4 cut(s)	90, 229, 853, 1277
Tru1I	TTAA	6 cut(s)	222, 576, 591, 779, 968, 1044
Tru9I	TTAA	6 cut(s)	222, 576, 591, 779, 968, 1044
TscAI	CASTG	5 cut(s)	322, 514, 628, 871, 1132
TseFI	GTSAC	1 cut(s)	134
TseI	GCWGC	1 cut(s)	70
Tsp45I	GTSAC	1 cut(s)	134
TspDTI	ATGAA	6 cut(s)	279, 644, 761, 995, 1023, 1323
TspGWI	ACGGA	2 cut(s)	102, 1216
TspRI	CASTG	5 cut(s)	322, 514, 628, 871, 1132
Vha464I	CTTAAG	1 cut(s)	575
VpaK11BI	GGWCC	4 cut(s)	547, 610, 991, 1061
VspI	ATTAAT	1 cut(s)	222
XapI	RAATTY	1 cut(s)	685
XbaI	TCTAGA	1 cut(s)	1088
XcmI	CCANNNNNNNNNTGG	2 cut(s)	422, 1124
XhoI	CTCGAG	3 cut(s)	6, 80, 110
XmiI	GTMKAC	1 cut(s)	1404
XmnI	GAANNNNTTC	1 cut(s)	105
XspI	CTAG	2 cut(s)	1089, 1152
ZrmI	AGTACT	1 cut(s)	1190
Zsp2I	ATGCAT	1 cut(s)	1393

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.