AT3G61740
ERF Family

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX MLL subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003180
Physical Location & Seq
Reverse (-)
22850811 .. 22857016
6206 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G61740.2

Sequence Viewer

Length: 3057 bp
ATGATTTTGAAACGGACCTTGACGACATTCGAGAATCAGAATCTGAAGCGGTGCAAAATTGATTCAGAAATTGAGTATGGGAGGAAAAAGGGTGAGATTATAGTGTATAAGAAGAGACAAAGAGCAACCGTGGATCAACCATGTAGCAAAGAACCAGAACTTCTTACTAGTAGCTCAAGCTCCTTGACAAGCAAAGAAGAATCTCAACAAGTTTGCTCTGACCAGTCCAAGTCTTCGCGTGGCAGAGTTCGAGCGGTTCCTTCTAGGTTCAAGGACTCCATTGTTGGTACATGGAAATCTAGCCGTCGCAAGGGAGAGTCGACGGAGTCTAGTCATGATGACGACGACGTATCTCTGGGGAAGAAGGTCAAAGGTTTCAGTGGAAGCTCGAAATTGCATCGCAGCAAAGACTCGAAGGTGTTTCCACGTAAGGATAACGGAGACAGCAGTGAAGTAGATTGCGATTACTGGGATGTTCAAATTTCCTACGACGATGCTAACTTTGGTATGCCGAAAAAGTCCGACGCCTCGCGTAAGGGCGTGTACAAACCAGAGGAATTTACGGTTGGCGATCTTGTGTGGGCCAAGTGTGGGAAAAGATTCCCAGCATGGCCGGCTGTGGTGATCGATCCGATATCACAAGCGCCTGATGGGGTCTTGAAACATTGCGTCCCTGGCGCAATTTGTGTCATGTTTTTTGGGTACTCCAAGGATGGAACTCAGAGGGACTATGCATGGGTAAGACAAGGGATGGTGTATCCATTTACGGAATTTATGGACAAATTTCAGGATCAGACAAACTTGTTCAATTACAAGGCAAGTGAATTTAACAAGGCACTAGAGGAAGCAGTTTTAGCAGAAAATGGTAATTTTGGAGATGCTGAAATCATCTCCCCAGATTCCTCTGCGACGGAATCCGACCAGGACTATGGACCTGCTTCTAGATTTCAGGGTTCATATCATGAGGACATAAGGACATGCGACGGTTGTGGCTCAGTAATGCCATTAAAATCTCTAAAAAGGACTAAAGACTCACAACCGGAAGAGCTTTTATGCAAACACTGCTCAAAGTTGCGGAAATCCAATCAGTATTGTGGCATCTGCAAGAGAATATGGCACCCTTCAGATGATGGAGATTGGGTTTGTTGTGATGGGTGTGACGTATGGGTACATGCTGAATGCGACAACATTACGAATGAACGCTTTAAGGAACTGGAGCACAACAATTACTATTGCCCTGATTGTAAAGTCCAACACGAGCTTACGCCAACAATATTAGAAGAACAGAACTCAGTGTTTAAGTCTACAGAAAAGACAACAGAGACTGGGCTGCCTGATGCGATTACTGTAGTCTGTAATGGCATGGAAGGGACATATATCAGAAAATTTCATGCAATTGAGTGCAAGTGTGGTTCATGTGGGTCAAGGAAGCAGTCACCAAGTGAATGGGAACGGCATACAGGCTGCAGAGCCAAAAAGTGGAAGTATAGTGTAAGAGTGAAAGACACAATGCTACCTCTAGAAAAATGGATTGCAGAATTCAGTACCTATACTCTAGAAACCCAGATGTTGGATAAGCAAAAGATGCTCTCTTTGCTAGAAGAAAAGTATGAGCCAGTCAGGGCTAAGTGGACGACTGAAAGGTGTGCTGTATGCAGATGGGTAGAAGACTGGGAAGAAAATAAAATGATCATCTGTAACAGGTGTCAAGTGGCTGTGCACCAAGAATGTTATGGGGTAAGCAAATCTCAGGACCTCACTTCCTGGGTATGCAGAGCATGTGAAACACCAGACATTGAGAGAGATTGTTGTCTTTGTCCTGTAAAAGGTGGTGCTTTAAAACCAAGCGACGTTGAGGGTTTGTGGGTTCATGTAACATGTGCTTGGTTCCGACCGGAAGTTGGGTTTTTGAATCATGAAAATATGGAGCCTGCTGTTGGACTTTTCAAAATTCCAGCAAATTCATTTCTTAAGGTTTGCACAATTTGTAAGCAGACACATGGTTCCTGCGTTCATTGCTGCAAGTGTGCAACTCACTTCCACGCAATGTGTGCATCACGGGCAGGTTACAACATGGAGTTGCACTGCCTGGAAAAAAATGGTGTGCAGAGAACCAGGAAGTCAGTTTACTGTTCATTTCACAGAAAGCCAGATCCAGATAGTGTTGTAGTTGTGCACACACCCTCAGGAGTATTTGGCTCTAGAAATTTGCTTCAGAATCAATATGGGCGCGCCAAAGGTTCAAGACTCGTTTTGACCAAGAAGATGAAGCTTCCAGGTTTCCAAACCCAAACCCAAGCTGAACAGAGTCGTGTATTTGATTCTCTTTCTGCAGCAAGATGCCGTATCTATAGTAGATCAAACACAAAGATTGATCTGGAGGCTATATCCCACAGGCTTAAGGGACCAAGCCATCATTCTTTGAGTGCAATCGAGAATCTAAACTCTTTTAAGGCAAGTTTCTCATTCCGAGCTCCATTCATGTCAGTGTTTTGCTTTCTTGGGGCAACGTTTTCTGAATATCTTAGGAAGATCTTAATTTCCATTTACCTTGTTACACACCAGGAAGCAGATTTTACATCATTCAGAGAACGGCTAAAACATTTGCAGAGGACAGAAAATTTTCGAGTCTGCTTTGGTAAATCTGGCATACATGGATGGGGTTTGTTTGCAAGGAAAAGTATTCAAGAAGGAGAAATGATCATCGAGTATCGTGGGGTGAAAGTTAGGAGAAGTGTTGCAGATCTGAGAGAGGCAAACTATCGTTCGCAGGGCAAAGATTGTTACCTATTCAAGATAAGCGAAGAGATAGTAATTGATGCCACAGACTCTGGGAACATCGCACGATTGATCAATCATTCATGTATGCCCAATTGCTATGCTCGTATAGTGAGTATGGGTGATGGAGAAGACAACAGAATCGTCCTCATTGCAAAAACCAATGTCGCAGCTGGGGAAGAGCTTACGTATGATTATCTCTTTGAGGTTGATGAATCTGAAGAGATCAAAGTGCCCTGCCTTTGTAAAGCTCCAAACTGCCGCAAATTCATGAACTAG

Protein Analysis

1018

Amino Acids

115.69

Weight (kDa)

8.31

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 190 - 285 1.3e-16 PWWP domain
PHD PF00628 365 - 415 1.7e-07 PHD-finger
PHD PF00628 549 - 594 1e-06 PHD-finger
PHD_2 PF13831 561 - 595 9.8e-14 PHD-finger
zf-HC5HC2H_2 PF13832 602 - 714 1.2e-25 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 624 - 715 2.6e-10 PHD-like zinc-binding domain
SET PF00856 850 - 956 1e-25 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 943, 2054
AccB1I GGYRCC 1 cut(s) 1116
AccB7I CCANNNNNTGG 2 cut(s) 1479, 1570
AccBSI CCGCTC 1 cut(s) 254
AccI GTMKAC 2 cut(s) 320, 1304
AccII CGCG 3 cut(s) 238, 532, 2232
AciI CCGC 4 cut(s) 49, 254, 1075, 3040
AclI AACGTT 1 cut(s) 2510
AclWI GGATC 4 cut(s) 141, 623, 798, 2147
AcoI YGGCCR 1 cut(s) 611
AcuI CTGAAG 4 cut(s) 65, 1107, 2198, 3018
AcyI GRCGYC 1 cut(s) 525
AdeI CACNNNGTG 1 cut(s) 1442
AfaI GTAC 5 cut(s) 289, 545, 704, 1170, 1546
AfiI CCNNNNNNNGG 7 cut(s) 310, 591, 1479, 1570, 1826, 1901, 1937
AflII CTTAAG 2 cut(s) 1970, 2399
AflIII ACRYGT 1 cut(s) 1877
AhlI ACTAGT 1 cut(s) 167
AjnI CCWGG 7 cut(s) 673, 921, 1763, 2088, 2114, 2275, 2562
AjuI GAANNNNNNNTTGG 4 cut(s) 549, 581, 2401, 2433
Alw21I GWGCWC 4 cut(s) 1221, 1722, 2178, 2476
Alw26I GTCTC 3 cut(s) 109, 435, 1316
Alw44I GTGCAC 2 cut(s) 1718, 2174
AlwI GGATC 4 cut(s) 141, 623, 798, 2147
AlwNI CAGNNNCTG 3 cut(s) 43, 1325, 2831
AoxI GGCC 2 cut(s) 582, 611
ApaLI GTGCAC 2 cut(s) 1718, 2174
ApeKI GCWGC 6 cut(s) 402, 1330, 1464, 2019, 2333, 2948
AscI GGCGCGCC 1 cut(s) 2230
Asp700I GAANNNNTTC 2 cut(s) 599, 2622
AspLEI GCGC 4 cut(s) 646, 680, 2232, 2234
AspS9I GGNCC 5 cut(s) 15, 582, 932, 1753, 2405
AsuHPI GGTGA 5 cut(s) 104, 634, 1428, 2731, 2912
AvaII GGWCC 4 cut(s) 15, 932, 1753, 2405
AxyI CCTNAGG 1 cut(s) 2185
BaeGI GKGCMC 3 cut(s) 1722, 2178, 3015
BanI GGYRCC 1 cut(s) 1116
BanII GRGCYC 1 cut(s) 2476
BarI GAAGNNNNNNTAC 2 cut(s) 2111, 2143
BauI CACGAG 1 cut(s) 1256
BbsI GAAGAC 3 cut(s) 225, 1674, 2916
Bbv12I GWGCWC 4 cut(s) 1221, 1722, 2178, 2476
BbvI GCAGC 6 cut(s) 414, 1317, 1451, 2006, 2345, 2960
BccI CCATC 9 cut(s) 644, 707, 745, 1124, 1145, 1653, 2421, 2652, 2897
BceAI ACGGC 4 cut(s) 288, 1469, 2328, 2609
BciT130I CCWGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
BciVI GTATCC 1 cut(s) 768
BclI TGATCA 3 cut(s) 1689, 2700, 2850
BcoDI GTCTC 3 cut(s) 109, 435, 1316
BcuI ACTAGT 1 cut(s) 167
BfmI CTRYAG 5 cut(s) 1305, 1347, 1465, 2331, 2350
BfoI RGCGCY 1 cut(s) 647
BfrI CTTAAG 2 cut(s) 1970, 2399
BfuAI ACCTGC 2 cut(s) 943, 2054
BfuI GTATCC 1 cut(s) 768
BglII AGATCT 2 cut(s) 2532, 2743
BisI GCNGC 7 cut(s) 403, 1331, 1465, 2020, 2334, 2949, 3040
BlsI GCNGC 7 cut(s) 404, 1332, 1466, 2021, 2335, 2950, 3041
Bme1390I CCNGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
Bme18I GGWCC 4 cut(s) 15, 932, 1753, 2405
BmgT120I GGNCC 5 cut(s) 15, 582, 932, 1753, 2405
BmiI GGNNCC 6 cut(s) 258, 1118, 1889, 1929, 2005, 2406
BmrFI CCNGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
BmrI ACTGGG 3 cut(s) 478, 1335, 1681
BmsI GCATC 9 cut(s) 406, 484, 868, 1107, 1327, 1575, 2063, 2330, 2809
BmuI ACTGGG 3 cut(s) 478, 1335, 1681
BpiI GAAGAC 3 cut(s) 225, 1674, 2916
BpmI CTGGAG 2 cut(s) 1235, 2399
BpuEI CTTGAG 1 cut(s) 160
Bsa29I ATCGAT 1 cut(s) 627
BsaAI YACGTR 2 cut(s) 428, 2967
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 4 cut(s) 129, 673, 708, 1764
BsaWI WCCGGW 2 cut(s) 1039, 1894
BsaXI ACNNNNNCTCC 2 cut(s) 1125, 1155
Bsc4I CCNNNNNNNGG 7 cut(s) 310, 591, 1479, 1570, 1826, 1901, 1937
Bse118I RCCGGY 1 cut(s) 613
Bse1I ACTGG 6 cut(s) 223, 473, 1218, 1330, 1616, 1676
Bse21I CCTNAGG 1 cut(s) 2185
Bse3DI GCAATG 4 cut(s) 664, 2014, 2052, 2928
BseBI CCWGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
BseCI ATCGAT 1 cut(s) 627
BseDI CCNNGG 4 cut(s) 129, 673, 708, 1764
BseGI GGATG 4 cut(s) 478, 718, 756, 2663
BseLI CCNNNNNNNGG 7 cut(s) 310, 591, 1479, 1570, 1826, 1901, 1937
BseMI GCAATG 4 cut(s) 664, 2014, 2052, 2928
BseMII CTCAG 6 cut(s) 734, 1008, 1305, 1763, 2199, 2738
BseNI ACTGG 6 cut(s) 223, 473, 1218, 1330, 1616, 1676
BsePI GCGCGC 1 cut(s) 2230
BseSI GKGCMC 3 cut(s) 1722, 2178, 3015
BseXI GCAGC 6 cut(s) 414, 1317, 1451, 2006, 2345, 2960
BseYI CCCAGC 2 cut(s) 604, 2951
BsgI GTGCAG 1 cut(s) 2126
Bsh1236I CGCG 3 cut(s) 238, 532, 2232
Bsh1285I CGRYCG 1 cut(s) 1895
BshFI GGCC 2 cut(s) 584, 613
BshNI GGYRCC 1 cut(s) 1116
BshVI ATCGAT 1 cut(s) 627
BsiEI CGRYCG 1 cut(s) 1895
BsiHKAI GWGCWC 4 cut(s) 1221, 1722, 2178, 2476
BsiSI CCGG 3 cut(s) 614, 1040, 1895
BslFI GGGAC 4 cut(s) 656, 740, 1384, 2418
BslI CCNNNNNNNGG 7 cut(s) 310, 591, 1479, 1570, 1826, 1901, 1937
BsmAI GTCTC 3 cut(s) 109, 435, 1316
BsmFI GGGAC 4 cut(s) 656, 740, 1384, 2418
BsmI GAATGC 1 cut(s) 1184
BsnI GGCC 2 cut(s) 584, 613
Bsp1286I GDGCHC 5 cut(s) 1221, 1722, 2178, 2476, 3015
Bsp1407I TGTACA 1 cut(s) 543
BspACI CCGC 4 cut(s) 49, 254, 1075, 3040
BspANI GGCC 2 cut(s) 584, 613
BspCNI CTCAG 6 cut(s) 733, 1007, 1304, 1762, 2198, 2739
BspDI ATCGAT 1 cut(s) 627
BspFNI CGCG 3 cut(s) 238, 532, 2232
BspHI TCATGA 4 cut(s) 334, 961, 1915, 3048
BspLI GGNNCC 6 cut(s) 258, 1118, 1889, 1929, 2005, 2406
BspMAI CTGCAG 2 cut(s) 1469, 2335
BspMI ACCTGC 2 cut(s) 943, 2054
BspPI GGATC 4 cut(s) 141, 623, 798, 2147
BspQI GCTCTTC 2 cut(s) 1038, 2952
BspT107I GGYRCC 1 cut(s) 1116
BspTI CTTAAG 2 cut(s) 1970, 2399
BsrBI CCGCTC 1 cut(s) 254
BsrDI GCAATG 4 cut(s) 664, 2014, 2052, 2928
BsrFI RCCGGY 1 cut(s) 613
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 6 cut(s) 223, 473, 1218, 1330, 1616, 1676
BssAI RCCGGY 1 cut(s) 613
BssECI CCNNGG 4 cut(s) 129, 673, 708, 1764
BssHII GCGCGC 1 cut(s) 2230
BssNI GRCGYC 1 cut(s) 525
BssSI CACGAG 1 cut(s) 1256
BssT1I CCWWGG 1 cut(s) 708
Bst2BI CACGAG 1 cut(s) 1256
Bst2UI CCWGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
Bst4CI ACNGT 5 cut(s) 130, 565, 986, 1348, 2132
Bst6I CTCTTC 5 cut(s) 107, 1038, 2799, 2952, 2994
BstACI GRCGYC 1 cut(s) 525
BstAFI CTTAAG 2 cut(s) 1970, 2399
BstAPI GCANNNNNTGC 3 cut(s) 1062, 1585, 2051
BstAUI TGTACA 1 cut(s) 543
BstBAI YACGTR 2 cut(s) 428, 2967
BstC8I GCNNGC 3 cut(s) 615, 1932, 2232
BstDEI CTNAG 8 cut(s) 720, 994, 1291, 1626, 1749, 2185, 2525, 2747
BstDSI CCRYGG 1 cut(s) 129
BstENI CCTNNNNNAGG 1 cut(s) 1824
BstF5I GGATG 4 cut(s) 478, 718, 756, 2663
BstFNI CGCG 3 cut(s) 238, 532, 2232
BstH2I RGCGCY 1 cut(s) 647
BstHHI GCGC 4 cut(s) 646, 680, 2232, 2234
BstMAI GTCTC 3 cut(s) 109, 435, 1316
BstMCI CGRYCG 1 cut(s) 1895
BstMWI GCNNNNNNNGC 9 cut(s) 614, 675, 854, 1062, 1585, 1594, 2016, 2051, 2060
BstNI CCWGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
BstNSI RCATGY 4 cut(s) 981, 1175, 1782, 1881
BstSCI CCNGG 7 cut(s) 673, 921, 1763, 2088, 2114, 2275, 2562
BstSFI CTRYAG 5 cut(s) 1305, 1347, 1465, 2331, 2350
BstSLI GKGCMC 3 cut(s) 1722, 2178, 3015
BstSNI TACGTA 1 cut(s) 2967
BstUI CGCG 3 cut(s) 238, 532, 2232
BstV1I GCAGC 6 cut(s) 414, 1317, 1451, 2006, 2345, 2960
BstV2I GAAGAC 3 cut(s) 225, 1674, 2916
BstX2I RGATCY 3 cut(s) 2152, 2532, 2743
BstXI CCANNNNNNTGG 2 cut(s) 929, 1446
BstYI RGATCY 3 cut(s) 2152, 2532, 2743
Bsu15I ATCGAT 1 cut(s) 627
Bsu36I CCTNAGG 1 cut(s) 2185
BsuI GTATCC 1 cut(s) 768
BsuRI GGCC 2 cut(s) 584, 613
BsuTUI ATCGAT 1 cut(s) 627
BtgI CCRYGG 1 cut(s) 129
BtgZI GCGATG 2 cut(s) 383, 2824
BtsCI GGATG 4 cut(s) 478, 718, 756, 2663
BtsI GCAGTG 3 cut(s) 454, 1060, 2083
BtsIMutI CAGTG 6 cut(s) 385, 454, 1060, 1299, 2083, 2493
BveI ACCTGC 2 cut(s) 943, 2054
Cac8I GCNNGC 3 cut(s) 615, 1932, 2232
CaiI CAGNNNCTG 3 cut(s) 43, 1325, 2831
CciI TCATGA 4 cut(s) 334, 961, 1915, 3048
CfoI GCGC 4 cut(s) 646, 680, 2232, 2234
Cfr10I RCCGGY 1 cut(s) 613
Cfr13I GGNCC 5 cut(s) 15, 582, 932, 1753, 2405
ClaI ATCGAT 1 cut(s) 627
CseI GACGC 2 cut(s) 533, 658
Csp6I GTAC 5 cut(s) 288, 544, 703, 1169, 1545
CviQI GTAC 5 cut(s) 288, 544, 703, 1169, 1545
DdeI CTNAG 8 cut(s) 720, 994, 1291, 1626, 1749, 2185, 2525, 2747
DraI TTTAAA 1 cut(s) 1839
DraIII CACNNNGTG 1 cut(s) 1442
EaeI YGGCCR 1 cut(s) 611
Eam1104I CTCTTC 5 cut(s) 107, 1038, 2799, 2952, 2994
EarI CTCTTC 5 cut(s) 107, 1038, 2799, 2952, 2994
Ecl136II GAGCTC 1 cut(s) 2474
Eco105I TACGTA 1 cut(s) 2967
Eco130I CCWWGG 1 cut(s) 708
Eco24I GRGCYC 1 cut(s) 2476
Eco32I GATATC 1 cut(s) 636
Eco47I GGWCC 4 cut(s) 15, 932, 1753, 2405
Eco53kI GAGCTC 1 cut(s) 2474
Eco57I CTGAAG 4 cut(s) 65, 1107, 2198, 3018
Eco81I CCTNAGG 1 cut(s) 2185
EcoICRI GAGCTC 1 cut(s) 2474
EcoNI CCTNNNNNAGG 1 cut(s) 1824
EcoO109I RGGNCCY 1 cut(s) 1753
EcoRI GAATTC 1 cut(s) 1538
EcoRII CCWGG 7 cut(s) 673, 921, 1763, 2088, 2114, 2275, 2562
EcoRV GATATC 1 cut(s) 636
EcoT14I CCWWGG 1 cut(s) 708
EcoT22I ATGCAT 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 2476
ErhI CCWWGG 1 cut(s) 708
FaqI GGGAC 4 cut(s) 656, 740, 1384, 2418
FbaI TGATCA 3 cut(s) 1689, 2700, 2850
FblI GTMKAC 2 cut(s) 320, 1304
Fnu4HI GCNGC 7 cut(s) 403, 1331, 1465, 2020, 2334, 2949, 3040
FokI GGATG 4 cut(s) 485, 725, 763, 2670
FriOI GRGCYC 1 cut(s) 2476
Fsp4HI GCNGC 7 cut(s) 403, 1331, 1465, 2020, 2334, 2949, 3040
GlaI GCGC 4 cut(s) 645, 679, 2231, 2233
GluI GCNGC 7 cut(s) 403, 1331, 1465, 2020, 2334, 2949, 3040
GsaI CCCAGC 2 cut(s) 608, 2955
GsuI CTGGAG 2 cut(s) 1235, 2399
HaeII RGCGCY 1 cut(s) 647
HaeIII GGCC 2 cut(s) 584, 613
HapII CCGG 3 cut(s) 614, 1040, 1895
HgaI GACGC 2 cut(s) 533, 658
HhaI GCGC 4 cut(s) 646, 680, 2232, 2234
Hin1I GRCGYC 1 cut(s) 525
Hin6I GCGC 4 cut(s) 644, 678, 2230, 2232
HinP1I GCGC 4 cut(s) 644, 678, 2230, 2232
HincII GTYRAC 1 cut(s) 321
HindII GTYRAC 1 cut(s) 321
HindIII AAGCTT 1 cut(s) 2270
HpaII CCGG 3 cut(s) 614, 1040, 1895
HphI GGTGA 5 cut(s) 104, 634, 1428, 2731, 2912
Hpy166II GTNNAC 7 cut(s) 321, 544, 1305, 1632, 1720, 2128, 2176
Hpy8I GTNNAC 7 cut(s) 321, 544, 1305, 1632, 1720, 2128, 2176
Hpy99I CGWCG 9 cut(s) 309, 325, 347, 350, 494, 527, 913, 986, 1853
HpyAV CCTTC 6 cut(s) 270, 358, 409, 1131, 1361, 2684
HpyCH4III ACNGT 5 cut(s) 130, 565, 986, 1348, 2132
HpyCH4IV ACGT 6 cut(s) 348, 427, 1161, 1851, 2510, 2966
HpyF10VI GCNNNNNNNGC 9 cut(s) 614, 675, 854, 1062, 1585, 1594, 2016, 2051, 2060
HpyF3I CTNAG 8 cut(s) 720, 994, 1291, 1626, 1749, 2185, 2525, 2747
HpySE526I ACGT 6 cut(s) 348, 427, 1161, 1851, 2510, 2966
Hsp92I GRCGYC 1 cut(s) 525
HspAI GCGC 4 cut(s) 644, 678, 2230, 2232
KroI GCCGGC 1 cut(s) 613
KroNI GCCGGC 1 cut(s) 615
Ksp22I TGATCA 3 cut(s) 1689, 2700, 2850
LguI GCTCTTC 2 cut(s) 1038, 2952
LmnI GCTCC 5 cut(s) 185, 1216, 1927, 2479, 3034
Lsp1109I GCAGC 6 cut(s) 414, 1317, 1451, 2006, 2345, 2960
LweI GCATC 9 cut(s) 406, 484, 868, 1107, 1327, 1575, 2063, 2330, 2809
MaeII ACGT 6 cut(s) 348, 427, 1161, 1851, 2510, 2966
MaeIII GTNAC 7 cut(s) 1157, 1434, 1697, 1873, 2066, 2554, 2783
MbiI CCGCTC 1 cut(s) 254
MfeI CAATTG 2 cut(s) 1395, 2872
MflI RGATCY 3 cut(s) 2152, 2532, 2743
MhlI GDGCHC 5 cut(s) 1221, 1722, 2178, 2476, 3015
MlyI GAGTC 9 cut(s) 269, 326, 335, 404, 1025, 2241, 2317, 2637, 2822
MmeI TCCRAC 6 cut(s) 546, 942, 1276, 1551, 1915, 1918
Mph1103I ATGCAT 1 cut(s) 736
MroNI GCCGGC 1 cut(s) 613
MroXI GAANNNNTTC 2 cut(s) 599, 2622
MseI TTAA 9 cut(s) 828, 1007, 1206, 1299, 1838, 1971, 2400, 2451, 2537
MslI CAYNNNNRTG 1 cut(s) 2486
MspA1I CMGCKG 1 cut(s) 2951
MspCI CTTAAG 2 cut(s) 1970, 2399
MspI CCGG 3 cut(s) 614, 1040, 1895
MspR9I CCNGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
MunI CAATTG 2 cut(s) 1395, 2872
Mva1269I GAATGC 1 cut(s) 1184
MvaI CCWGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
MvnI CGCG 3 cut(s) 238, 532, 2232
MwoI GCNNNNNNNGC 9 cut(s) 614, 675, 854, 1062, 1585, 1594, 2016, 2051, 2060
NaeI GCCGGC 1 cut(s) 615
NgoMIV GCCGGC 1 cut(s) 613
NlaIV GGNNCC 6 cut(s) 258, 1118, 1889, 1929, 2005, 2406
NmuCI GTSAC 2 cut(s) 1157, 1434
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 4 cut(s) 981, 1175, 1782, 1881
PagI TCATGA 4 cut(s) 334, 961, 1915, 3048
PalAI GGCGCGCC 1 cut(s) 2230
PauI GCGCGC 1 cut(s) 2230
PciI ACATGT 1 cut(s) 1877
PciSI GCTCTTC 2 cut(s) 1038, 2952
PctI GAATGC 1 cut(s) 1184
PdiI GCCGGC 1 cut(s) 615
PdmI GAANNNNTTC 2 cut(s) 599, 2622
PflFI GACNNNGTC 1 cut(s) 325
PflMI CCANNNNNTGG 2 cut(s) 1479, 1570
PkrI GCNGC 7 cut(s) 404, 1332, 1466, 2021, 2335, 2950, 3041
PleI GAGTC 9 cut(s) 269, 325, 334, 404, 1025, 2241, 2316, 2636, 2822
PpsI GAGTC 9 cut(s) 269, 325, 334, 404, 1025, 2241, 2316, 2636, 2822
Ppu21I YACGTR 2 cut(s) 428, 2967
PpuMI RGGWCCY 1 cut(s) 1753
PscI ACATGT 1 cut(s) 1877
Psp124BI GAGCTC 1 cut(s) 2476
Psp1406I AACGTT 1 cut(s) 2510
Psp5II RGGWCCY 1 cut(s) 1753
Psp6I CCWGG 7 cut(s) 673, 921, 1763, 2088, 2114, 2275, 2562
PspFI CCCAGC 2 cut(s) 604, 2951
PspGI CCWGG 7 cut(s) 673, 921, 1763, 2088, 2114, 2275, 2562
PspN4I GGNNCC 6 cut(s) 258, 1118, 1889, 1929, 2005, 2406
PspPI GGNCC 5 cut(s) 15, 582, 932, 1753, 2405
PspPPI RGGWCCY 1 cut(s) 1753
PstI CTGCAG 2 cut(s) 1469, 2335
PstNI CAGNNNCTG 3 cut(s) 43, 1325, 2831
PsuI RGATCY 3 cut(s) 2152, 2532, 2743
PsyI GACNNNGTC 1 cut(s) 325
PteI GCGCGC 1 cut(s) 2230
PvuII CAGCTG 1 cut(s) 2951
RsaI GTAC 5 cut(s) 289, 545, 704, 1170, 1546
RsaNI GTAC 5 cut(s) 288, 544, 703, 1169, 1545
RseI CAYNNNNRTG 1 cut(s) 2486
SacI GAGCTC 1 cut(s) 2476
SalI GTCGAC 1 cut(s) 319
SapI GCTCTTC 2 cut(s) 1038, 2952
SaqAI TTAA 9 cut(s) 828, 1007, 1206, 1299, 1838, 1971, 2400, 2451, 2537
SatI GCNGC 7 cut(s) 403, 1331, 1465, 2020, 2334, 2949, 3040
Sau96I GGNCC 5 cut(s) 15, 582, 932, 1753, 2405
SchI GAGTC 9 cut(s) 269, 326, 335, 404, 1025, 2241, 2317, 2637, 2822
ScrFI CCNGG 7 cut(s) 675, 923, 1765, 2090, 2116, 2277, 2564
SduI GDGCHC 5 cut(s) 1221, 1722, 2178, 2476, 3015
SfaNI GCATC 9 cut(s) 406, 484, 868, 1107, 1327, 1575, 2063, 2330, 2809
SfcI CTRYAG 5 cut(s) 1305, 1347, 1465, 2331, 2350
SgsI GGCGCGCC 1 cut(s) 2230
SinI GGWCC 4 cut(s) 15, 932, 1753, 2405
SmiMI CAYNNNNRTG 1 cut(s) 2486
SmlI CTYRAG 3 cut(s) 175, 1970, 2399
SmoI CTYRAG 3 cut(s) 175, 1970, 2399
SnaBI TACGTA 1 cut(s) 2967
SpeI ACTAGT 1 cut(s) 167
SsiI CCGC 4 cut(s) 49, 254, 1075, 3040
SspI AATATT 1 cut(s) 1275
SstI GAGCTC 1 cut(s) 2476
StyD4I CCNGG 7 cut(s) 673, 921, 1763, 2088, 2114, 2275, 2562
StyI CCWWGG 1 cut(s) 708
TaaI ACNGT 5 cut(s) 130, 565, 986, 1348, 2132
TaiI ACGT 6 cut(s) 351, 430, 1164, 1854, 2513, 2969
TaqI TCGA 9 cut(s) 30, 250, 320, 389, 413, 627, 2433, 2626, 2706
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 3042
Tru1I TTAA 9 cut(s) 828, 1007, 1206, 1299, 1838, 1971, 2400, 2451, 2537
Tru9I TTAA 9 cut(s) 828, 1007, 1206, 1299, 1838, 1971, 2400, 2451, 2537
TscAI CASTG 6 cut(s) 385, 454, 1067, 1299, 2090, 2493
TseFI GTSAC 2 cut(s) 1157, 1434
TseI GCWGC 6 cut(s) 402, 1330, 1464, 2019, 2333, 2948
Tsp45I GTSAC 2 cut(s) 1157, 1434
TspGWI ACGGA 5 cut(s) 28, 338, 453, 782, 926
TspRI CASTG 6 cut(s) 385, 454, 1067, 1299, 2090, 2493
Tth111I GACNNNGTC 1 cut(s) 325
Van91I CCANNNNNTGG 2 cut(s) 1479, 1570
Vha464I CTTAAG 2 cut(s) 1970, 2399
VneI GTGCAC 2 cut(s) 1718, 2174
VpaK11BI GGWCC 4 cut(s) 15, 932, 1753, 2405
XagI CCTNNNNNAGG 1 cut(s) 1824
XbaI TCTAGA 4 cut(s) 941, 1519, 1555, 2201
XceI RCATGY 4 cut(s) 981, 1175, 1782, 1881
XcmI CCANNNNNNNNNTGG 1 cut(s) 1730
XmiI GTMKAC 2 cut(s) 320, 1304
XmnI GAANNNNTTC 2 cut(s) 599, 2622
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.