Rh1DG279200
ERF Family

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX MLL subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003180
Physical Location & Seq
Reverse (-)
50066439 .. 50074121
7683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG279200.1

Sequence Viewer

Length: 3129 bp
ATGATAATTAAGCGGCCGTCGAGATTTGAAATGCCGAATTTGAAACGGTGTAAGCTGGAGGAGGCGGAAGCTAAAGCCTATGCGTATTCGGCGAATCCGAAGAGGCGGAAAGCGAATGGGTACTGCTCAGTTGGCAATGGCGAGGGGGAGGATTTGAGCAGCGGCTCCGGTTCGTCGGGATACGACGAGTCGTTTTCGGCTGCGGTCGAGGTGGTGGAGTCGAATTTGCAGCGGTTGAGTAACCGGAGAGAAATGAATCGGAGGTCCGAGAAGTTGAGGAGGTCAGCTCGAGGACGGCCGCAAGTGCCGTCTTCGAGGTTTAACGGTTCAGGTGGGAAAAAGCAAGCGAAATATGTAGATACATATACGGTTTTTGAAGAAGAAGAATGTGATGTTTTAGCAGTGGAGGACAGAGAAGGGATTGAGAAATTGGGTTTTGTTAAAAAGCGAAAGGAGGATATCAATGGGTTTAAGAAGTTGAAGAAGTTCCAATATTGTGGGAAGGTAGAGCAAGAAGAAGTGGATTATTATGATGATATTGGTTACAGACAGTATATGGGTTTGAAAAATTCGATAGCGTCGAAACGTGGTGGAAATTCTTTGCCAGAGGAAAGAAGTGAGGAATATGGAGCTGGGTTTAGTTTTGGCGGCAAGGAGAAAGTTGGCAAACCCGATTGTGCAAAGAAGAAAGAAGTGTATAAGCCGCAGGACTTTGCTTTGGGTGATATTGTTTGGGCTAAGTGTGGGAGGAGCTACCCAGTTTGGCCCGCGGTGGTTATCGACCCCATATTGCAAGCTCCAGAAGCAGTGTTGAGCTGCTGTATTCCTAATGCTATCTGTGTCATGTTCTTTGGGTTCTCAAAGAATGGAACACAAAGGGATTATGGGTGGGTGAAGCAGGGAATGATATATCCGTTCAAGCAGTTCCTTGAGAGATTTCGAGGACAGACCCAGTTGTACAGGAGTAAACCAAGCGAGCTTCAGATGGCAATAGAGGAGGCGGTGTTAGCAGAAAATGGCTTTATTTACGCAGCTTGTAAAACTACTGATTCAGAGGCAAATGCCCCTGAAATCCAAGAGGCCACAGATTTTTATCAAGATCAGGCATATTACAGTCAGAATCTGAATCAGGAAGCATATCAGAAGGACACAAGACCTTGTGATTGCTGTGGTTTGATCACTCCATCTAAAGCTATGAAAAAAGTGAAGGGCTCAGGTTCTGAAACCCAAGTTTTATGTAAACATTGTGCAAAGTTGAAAAAATCAAGACAATATTGTGGCATATGTAAGAAGATATGGCACCATTCAGATGGTGGAGATTGGGTATGCTGTGATGGCTGTAATGTTTGGGTGCATGCTGAGTGTGACAAAATTTCCAGTAAAGTTTTCAAGGACCCAGAGCACATTGATTACTATTGTCCTGATTGCAAAAATAAGGCCAAAGGTGAAATGTTAGATGAGTTACAGTGTCGGCAAAAACAGAGTTCTGGCCGGAAATCTGTGACTGGCGTAACTGTGTCTGAGGTAACTGTGGTTTGTAATGGCATGGAAGGAACTTATGTACCAAACCTTCATATGGTTGCTTGTAAGTGTGGACCCTGTGATGAGTCAAAGATGCAGAGCCTTGGTGAATGGGAAAGGCACACAGGTTGTAGAGCAAAAAAATGGAAGCATAGTGTGAAAGTGAAGGCCACAATGCTAACACTGGAACAATGGATTGCAGAGTATAATGAACATGGCTTACAGCCTCTCAAATTAGATAAGCAGCGACTACTTGATTCCTTACAAGAAAAGTACGAGCCAGTTAATGCAAAGTGGACAAGTGAAAGATGTGCAGTTTGTAGATGGGTTGAAGATTGGGAAGATAACAAAATGATCATATGCAACAGGTGTCAAATAGCTGTGCACCAAGAATGCTATGGTGCAAAAGATGTCCAGGATTTTACTTCATGGGTTTGTAGAGCATGTGAAACACCTGATATCATTAAAGAATGTTGTCTCTGTCCTGTAAAAGGAGGTGCTTTGAAGCCCACTGATGTTGACACGTTGTGGGTTCATGTCTCTTGTGCTTGGTTTCGTCCCGAAGTAGGTTTCTTGAATCATGAGAAGATGGAACCTGCTGTTGGAATCCTTAGAATCCCACCCACTACTTTTATGAAGCGTTGCGTAATTTGTACGCAGACTCATGGTTCCTGCACCACATGTTGCAAATGTGCCACTCATTTTCATGCAACCTGTGCATCAAGAGCAGGCTACAACATGGAATTGCACTGCCAAGAGAAGAATGGGAAACAAGTAACAACGAAGATGATATATTGTGCTGTGCACAGGACCCCTAATCCAGATGCTGTTGTCGTTGTTCGTACTCCTTCGGGAGTTTTTGCTGCCAGAAGCTTGCTTCAAAACGAGAAAGGGTTCTTCAGGGGTTCGAGGGTGGTCTCATCAAAGAGGACTGAAGTTCCAGAACCTTCAACCTCAGAGTCTGATGAGTTAGAACCACTCTCTGCTGCAAGATGCCGTGCTATTAAAAGATCAAATTTTAAGGGAGGTGAGCGGCTACCAATTTTTCATCGACCAAGGGGACCAACACATCATTCATTAGACTCAGTGAATAGCTTGAGCACATTTATAGAAGTAGAAGATTCTAAAATTTTTTCTTCCTTCAAGGATCGGCTTTACCACTTGCAGAAAACTGAAAATCACCGGGTTTGCTTTGGTAAATCTGGAATACATGGATGGGGTCTATTTGCACGTAGGAATATTCAAGAAGGAGAAATGGTTGTTGAGTATCGTGGTGAGCGGGTAAGACGTAGTGTTGCAGATCTGAGGGAAGCACGGTACAGGCTACAAGGCAAAGATTGCTATCTTTTCAAGATCAGCGAGGAAGTAGTAATTGATGCTACATATAGAGGAAATATAGCACGGTTAATTAACCATTCGTGCACACCCAATTGCTATTCAAGGATCATGAGTGTGGGTGAGGAGAACCGAATAGTTCTCATTGCCAAGACTAGTGTTTCGGCCGGTGAAGAACTAACGTACGATTATTTGTTTGAGCCGGATGAATGTGACGAGCTGAGAGTTCCCTGTCTATGTAAAGCTCCCAACTGTAGGAAATTCTTGTGTTAG

Protein Analysis

1042

Amino Acids

118.11

Weight (kDa)

8.56

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 241 - 336 7.3e-15 PWWP domain
PHD PF00628 426 - 476 6.7e-07 PHD-finger
PHD PF00628 611 - 656 9.5e-06 PHD-finger
PHD_2 PF13831 623 - 657 1.4e-12 PHD-finger
zf-HC5HC2H_2 PF13832 664 - 776 5.4e-26 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 686 - 777 1.2e-10 PHD-like zinc-binding domain
SET PF00856 912 - 1016 1e-26 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2125
AccB1I GGYRCC 1 cut(s) 1299
AccBSI CCGCTC 2 cut(s) 2554, 2800
AccII CGCG 1 cut(s) 770
AclWI GGATC 2 cut(s) 2676, 2972
AcoI YGGCCR 4 cut(s) 14, 296, 1489, 3021
AcsI RAATTY 8 cut(s) 37, 223, 568, 595, 1371, 2536, 2649, 3116
AcuI CTGAAG 3 cut(s) 965, 2404, 2475
AdeI CACNNNGTG 1 cut(s) 2049
AfaI GTAC 8 cut(s) 122, 959, 1563, 1796, 2176, 2365, 2840, 3041
AfiI CCNNNNNNNGG 5 cut(s) 1576, 2012, 2087, 2123, 3111
AflIII ACRYGT 2 cut(s) 2043, 2201
AhlI ACTAGT 1 cut(s) 3011
AjnI CCWGG 1 cut(s) 1935
AjuI GAANNNNNNNTTGG 4 cut(s) 2106, 2138, 2578, 2610
Alw21I GWGCWC 5 cut(s) 1404, 1908, 2328, 2624, 2945
Alw26I GTCTC 3 cut(s) 2003, 2065, 2443
Alw44I GTGCAC 3 cut(s) 1904, 2324, 2941
AlwI GGATC 2 cut(s) 2676, 2972
AlwNI CAGNNNCTG 4 cut(s) 1123, 1220, 2348, 2483
Ama87I CYCGRG 1 cut(s) 288
AoxI GGCC 8 cut(s) 14, 296, 764, 1080, 1437, 1489, 1689, 3021
ApaLI GTGCAC 3 cut(s) 1904, 2324, 2941
ApeKI GCWGC 8 cut(s) 159, 200, 229, 816, 1031, 1765, 2384, 2507
ApoI RAATTY 8 cut(s) 37, 223, 568, 595, 1371, 2536, 2649, 3116
ArsI GACNNNNNNTTYG 2 cut(s) 3001, 3033
Asp700I GAANNNNTTC 2 cut(s) 485, 2414
AspS9I GGNCC 6 cut(s) 264, 765, 1393, 1595, 2331, 2582
AsuC2I CCSGG 1 cut(s) 2705
AsuHPI GGTGA 9 cut(s) 734, 904, 1457, 1640, 2561, 2693, 2807, 2990, 3038
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 5 cut(s) 264, 1393, 1595, 2331, 2582
BaeGI GKGCMC 3 cut(s) 1908, 2328, 2945
BaeI ACNNNNGTAYC 2 cut(s) 1545, 1578
BanI GGYRCC 1 cut(s) 1299
BanII GRGCYC 1 cut(s) 1214
BarI GAAGNNNNNNTAC 2 cut(s) 1554, 1586
BbsI GAAGAC 1 cut(s) 303
Bbv12I GWGCWC 5 cut(s) 1404, 1908, 2328, 2624, 2945
BbvI GCAGC 8 cut(s) 171, 187, 241, 803, 1043, 1777, 2371, 2494
BccI CCATC 7 cut(s) 979, 1192, 1304, 1328, 1839, 2104, 2730
BceAI ACGGC 3 cut(s) 292, 311, 2502
BciT130I CCWGG 1 cut(s) 1937
BciVI GTATCC 1 cut(s) 173
BclI TGATCA 2 cut(s) 1176, 1875
BcnI CCSGG 1 cut(s) 2705
BcoDI GTCTC 3 cut(s) 2003, 2065, 2443
BcuI ACTAGT 1 cut(s) 3011
BfaI CTAG 1 cut(s) 3012
BfmI CTRYAG 1 cut(s) 3109
BfuAI ACCTGC 1 cut(s) 2125
BfuI GTATCC 1 cut(s) 173
BglII AGATCT 1 cut(s) 2821
Bme1390I CCNGG 2 cut(s) 1937, 2705
Bme18I GGWCC 5 cut(s) 264, 1393, 1595, 2331, 2582
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 6 cut(s) 264, 765, 1393, 1595, 2331, 2582
BmiI GGNNCC 8 cut(s) 166, 1301, 1395, 1597, 2115, 2191, 2333, 2583
BmrFI CCNGG 2 cut(s) 1937, 2705
BmrI ACTGGG 2 cut(s) 752, 946
BmsI GCATC 5 cut(s) 1605, 2249, 2335, 2504, 2887
BmuI ACTGGG 2 cut(s) 752, 946
BpiI GAAGAC 1 cut(s) 303
BplI GAGNNNNNCTC 2 cut(s) 271, 303
BpmI CTGGAG 2 cut(s) 77, 783
Bpu10I CCTNAGC 1 cut(s) 1213
BpuEI CTTGAG 2 cut(s) 950, 2638
BpuMI CCSGG 1 cut(s) 2705
BsaAI YACGTR 1 cut(s) 2753
BsaBI GATNNNNATC 2 cut(s) 1092, 2862
BsaI GGTCTC 1 cut(s) 2443
BsaJI CCNNGG 3 cut(s) 768, 1624, 2576
BsaWI WCCGGW 2 cut(s) 167, 243
BsaXI ACNNNNNCTCC 6 cut(s) 989, 1019, 1308, 1338, 2763, 2793
Bsc4I CCNNNNNNNGG 5 cut(s) 1576, 2012, 2087, 2123, 3111
Bse118I RCCGGY 1 cut(s) 3023
Bse1I ACTGG 6 cut(s) 758, 952, 1377, 1510, 1710, 1802
Bse3DI GCAATG 2 cut(s) 142, 3000
Bse8I GATNNNNATC 2 cut(s) 1092, 2862
BseBI CCWGG 1 cut(s) 1937
BseDI CCNNGG 3 cut(s) 768, 1624, 2576
BseGI GGATG 2 cut(s) 2741, 3067
BseJI GATNNNNATC 2 cut(s) 1092, 2862
BseLI CCNNNNNNNGG 5 cut(s) 1576, 2012, 2087, 2123, 3111
BseMI GCAATG 2 cut(s) 142, 3000
BseMII CTCAG 8 cut(s) 141, 1227, 1350, 1512, 2490, 2619, 2816, 3068
BseNI ACTGG 6 cut(s) 758, 952, 1377, 1510, 1710, 1802
BseRI GAGGAG 5 cut(s) 74, 292, 763, 1010, 2996
BseSI GKGCMC 3 cut(s) 1908, 2328, 2945
BseX3I CGGCCG 3 cut(s) 14, 296, 3021
BseXI GCAGC 8 cut(s) 171, 187, 241, 803, 1043, 1777, 2371, 2494
BseYI CCCAGC 1 cut(s) 632
BsgI GTGCAG 2 cut(s) 1854, 2179
Bsh1236I CGCG 1 cut(s) 770
Bsh1285I CGRYCG 4 cut(s) 17, 207, 299, 3024
BshFI GGCC 8 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691, 3023
BshNI GGYRCC 1 cut(s) 1299
BsiEI CGRYCG 4 cut(s) 17, 207, 299, 3024
BsiHKAI GWGCWC 5 cut(s) 1404, 1908, 2328, 2624, 2945
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 6 cut(s) 168, 244, 1492, 2704, 3024, 3059
BsiWI CGTACG 1 cut(s) 3039
BslFI GGGAC 2 cut(s) 2064, 2595
BslI CCNNNNNNNGG 5 cut(s) 1576, 2012, 2087, 2123, 3111
BsmAI GTCTC 3 cut(s) 2003, 2065, 2443
BsmFI GGGAC 2 cut(s) 2064, 2595
BsmI GAATGC 1 cut(s) 1919
BsnI GGCC 8 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691, 3023
Bso31I GGTCTC 1 cut(s) 2443
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 6 cut(s) 1214, 1404, 1908, 2328, 2624, 2945
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 8 cut(s) 1099, 1176, 1875, 2531, 2668, 2821, 2874, 2964
BspANI GGCC 8 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691, 3023
BspCNI CTCAG 8 cut(s) 140, 1226, 1351, 1513, 2489, 2618, 2817, 3069
BspFNI CGCG 1 cut(s) 770
BspHI TCATGA 2 cut(s) 2101, 2967
BspLI GGNNCC 8 cut(s) 166, 1301, 1395, 1597, 2115, 2191, 2333, 2583
BspMI ACCTGC 1 cut(s) 2125
BspPI GGATC 2 cut(s) 2676, 2972
BspT107I GGYRCC 1 cut(s) 1299
BspTNI GGTCTC 1 cut(s) 2443
BsrBI CCGCTC 2 cut(s) 2554, 2800
BsrDI GCAATG 2 cut(s) 142, 3000
BsrFI RCCGGY 1 cut(s) 3023
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 6 cut(s) 758, 952, 1377, 1510, 1710, 1802
BssAI RCCGGY 1 cut(s) 3023
BssECI CCNNGG 3 cut(s) 768, 1624, 2576
BssMI GATC 8 cut(s) 1099, 1176, 1875, 2531, 2668, 2821, 2874, 2964
BssT1I CCWWGG 2 cut(s) 1624, 2576
Bst2UI CCWGG 1 cut(s) 1937
Bst6I CTCTTC 1 cut(s) 95
BstAPI GCANNNNNTGC 2 cut(s) 2237, 2859
BstAUI TGTACA 1 cut(s) 957
BstBAI YACGTR 1 cut(s) 2753
BstC8I GCNNGC 7 cut(s) 345, 768, 795, 977, 1356, 2251, 2396
BstDSI CCRYGG 1 cut(s) 768
BstENI CCTNNNNNAGG 1 cut(s) 2010
BstF5I GGATG 2 cut(s) 2741, 3067
BstFNI CGCG 1 cut(s) 770
BstKTI GATC 8 cut(s) 1102, 1179, 1878, 2534, 2671, 2824, 2877, 2967
BstMAI GTCTC 3 cut(s) 2003, 2065, 2443
BstMBI GATC 8 cut(s) 1099, 1176, 1875, 2531, 2668, 2821, 2874, 2964
BstMCI CGRYCG 4 cut(s) 17, 207, 299, 3024
BstMWI GCNNNNNNNGC 9 cut(s) 89, 132, 304, 803, 1007, 1335, 2237, 2246, 2859
BstNI CCWGG 1 cut(s) 1937
BstNSI RCATGY 3 cut(s) 1358, 1968, 2205
BstSCI CCNGG 2 cut(s) 1935, 2703
BstSFI CTRYAG 1 cut(s) 3109
BstSLI GKGCMC 3 cut(s) 1908, 2328, 2945
BstUI CGCG 1 cut(s) 770
BstV1I GCAGC 8 cut(s) 171, 187, 241, 803, 1043, 1777, 2371, 2494
BstV2I GAAGAC 1 cut(s) 303
BstX2I RGATCY 1 cut(s) 2821
BstXI CCANNNNNNTGG 2 cut(s) 497, 1310
BstYI RGATCY 1 cut(s) 2821
BstZI CGGCCG 3 cut(s) 14, 296, 3021
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 8 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691, 3023
BtgI CCRYGG 1 cut(s) 768
BtsCI GGATG 2 cut(s) 2741, 3067
BtsI GCAGTG 3 cut(s) 408, 813, 2269
BtsIMutI CAGTG 7 cut(s) 408, 813, 1472, 1703, 2031, 2269, 2613
BveI ACCTGC 1 cut(s) 2125
Cac8I GCNNGC 7 cut(s) 345, 768, 795, 977, 1356, 2251, 2396
CaiI CAGNNNCTG 4 cut(s) 1123, 1220, 2348, 2483
CciI TCATGA 2 cut(s) 2101, 2967
Cfr10I RCCGGY 1 cut(s) 3023
Cfr13I GGNCC 6 cut(s) 264, 765, 1393, 1595, 2331, 2582
Cfr42I CCGCGG 1 cut(s) 771
CseI GACGC 1 cut(s) 567
Csp6I GTAC 8 cut(s) 121, 958, 1562, 1795, 2175, 2364, 2839, 3040
CspCI CAANNNNNGTGG 2 cut(s) 2020, 2055
CviQI GTAC 8 cut(s) 121, 958, 1562, 1795, 2175, 2364, 2839, 3040
DpnI GATC 8 cut(s) 1101, 1178, 1877, 2533, 2670, 2823, 2876, 2966
DpnII GATC 8 cut(s) 1099, 1176, 1875, 2531, 2668, 2821, 2874, 2964
DraIII CACNNNGTG 1 cut(s) 2049
EaeI YGGCCR 4 cut(s) 14, 296, 1489, 3021
EagI CGGCCG 3 cut(s) 14, 296, 3021
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
EciI GGCGGA 2 cut(s) 80, 121
EclXI CGGCCG 3 cut(s) 14, 296, 3021
Eco130I CCWWGG 2 cut(s) 1624, 2576
Eco24I GRGCYC 1 cut(s) 1214
Eco31I GGTCTC 1 cut(s) 2443
Eco32I GATATC 2 cut(s) 460, 1981
Eco47I GGWCC 5 cut(s) 264, 1393, 1595, 2331, 2582
Eco52I CGGCCG 3 cut(s) 14, 296, 3021
Eco57I CTGAAG 3 cut(s) 965, 2404, 2475
Eco88I CYCGRG 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 2010
EcoO109I RGGNCCY 2 cut(s) 1393, 2331
EcoRII CCWGG 1 cut(s) 1935
EcoRV GATATC 2 cut(s) 460, 1981
EcoT14I CCWWGG 2 cut(s) 1624, 2576
EcoT38I GRGCYC 1 cut(s) 1214
ErhI CCWWGG 2 cut(s) 1624, 2576
FaqI GGGAC 2 cut(s) 2064, 2595
FauI CCCGC 2 cut(s) 775, 2793
FauNDI CATATG 3 cut(s) 1283, 1575, 1880
FbaI TGATCA 2 cut(s) 1176, 1875
FokI GGATG 2 cut(s) 2748, 3074
FriOI GRGCYC 1 cut(s) 1214
FspBI CTAG 1 cut(s) 3012
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 2 cut(s) 77, 783
HaeIII GGCC 8 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691, 3023
HapII CCGG 6 cut(s) 168, 244, 1492, 2704, 3024, 3059
HgaI GACGC 1 cut(s) 567
HincII GTYRAC 1 cut(s) 2041
HindII GTYRAC 1 cut(s) 2041
HindIII AAGCTT 1 cut(s) 2392
HpaII CCGG 6 cut(s) 168, 244, 1492, 2704, 3024, 3059
HphI GGTGA 9 cut(s) 734, 904, 1457, 1640, 2561, 2693, 2807, 2990, 3038
Hpy166II GTNNAC 8 cut(s) 968, 1241, 1595, 1818, 1906, 2041, 2326, 2943
Hpy8I GTNNAC 8 cut(s) 968, 1241, 1595, 1818, 1906, 2041, 2326, 2943
Hpy99I CGWCG 4 cut(s) 22, 178, 188, 583
HpyCH4IV ACGT 5 cut(s) 586, 2045, 2752, 2809, 3038
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 132, 304, 803, 1007, 1335, 2237, 2246, 2859
HpySE526I ACGT 5 cut(s) 586, 2045, 2752, 2809, 3038
Ksp22I TGATCA 2 cut(s) 1176, 1875
KspI CCGCGG 1 cut(s) 771
Kzo9I GATC 8 cut(s) 1099, 1176, 1875, 2531, 2668, 2821, 2874, 2964
LmnI GCTCC 5 cut(s) 170, 629, 750, 802, 3106
Lsp1109I GCAGC 8 cut(s) 171, 187, 241, 803, 1043, 1777, 2371, 2494
LweI GCATC 5 cut(s) 1605, 2249, 2335, 2504, 2887
MaeI CTAG 1 cut(s) 3012
MaeII ACGT 5 cut(s) 586, 2045, 2752, 2809, 3038
MaeIII GTNAC 9 cut(s) 239, 542, 1364, 1461, 1501, 1510, 1525, 2296, 3068
MalI GATC 8 cut(s) 1101, 1178, 1877, 2533, 2670, 2823, 2876, 2966
MbiI CCGCTC 2 cut(s) 2554, 2800
MboI GATC 8 cut(s) 1099, 1176, 1875, 2531, 2668, 2821, 2874, 2964
MfeI CAATTG 1 cut(s) 2950
MflI RGATCY 1 cut(s) 2821
MhlI GDGCHC 6 cut(s) 1214, 1404, 1908, 2328, 2624, 2945
MlyI GAGTC 6 cut(s) 197, 227, 1616, 2176, 2489, 2597
MmeI TCCRAC 1 cut(s) 2104
MroXI GAANNNNTTC 2 cut(s) 485, 2414
MslI CAYNNNNRTG 3 cut(s) 1308, 2226, 2972
MspA1I CMGCKG 3 cut(s) 162, 232, 770
MspI CCGG 6 cut(s) 168, 244, 1492, 2704, 3024, 3059
MspR9I CCNGG 2 cut(s) 1937, 2705
MunI CAATTG 1 cut(s) 2950
Mva1269I GAATGC 1 cut(s) 1919
MvaI CCWGG 1 cut(s) 1937
MvnI CGCG 1 cut(s) 770
MwoI GCNNNNNNNGC 9 cut(s) 89, 132, 304, 803, 1007, 1335, 2237, 2246, 2859
NciI CCSGG 1 cut(s) 2705
NdeI CATATG 3 cut(s) 1283, 1575, 1880
NdeII GATC 8 cut(s) 1099, 1176, 1875, 2531, 2668, 2821, 2874, 2964
NlaIV GGNNCC 8 cut(s) 166, 1301, 1395, 1597, 2115, 2191, 2333, 2583
NmuCI GTSAC 3 cut(s) 1364, 1501, 3068
NspI RCATGY 3 cut(s) 1358, 1968, 2205
PacI TTAATTAA 1 cut(s) 2931
PaeI GCATGC 1 cut(s) 1358
PaeR7I CTCGAG 1 cut(s) 288
PagI TCATGA 2 cut(s) 2101, 2967
PciI ACATGT 1 cut(s) 2201
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 578
PctI GAATGC 1 cut(s) 1919
PdmI GAANNNNTTC 2 cut(s) 485, 2414
Pfl23II CGTACG 1 cut(s) 3039
PfoI TCCNGGA 1 cut(s) 1935
PleI GAGTC 6 cut(s) 196, 226, 1615, 2176, 2488, 2597
PpsI GAGTC 6 cut(s) 196, 226, 1615, 2176, 2488, 2597
Ppu21I YACGTR 1 cut(s) 2753
PpuMI RGGWCCY 2 cut(s) 1393, 2331
PscI ACATGT 1 cut(s) 2201
Psp5II RGGWCCY 2 cut(s) 1393, 2331
Psp6I CCWGG 1 cut(s) 1935
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 1 cut(s) 1935
PspLI CGTACG 1 cut(s) 3039
PspN4I GGNNCC 8 cut(s) 166, 1301, 1395, 1597, 2115, 2191, 2333, 2583
PspPI GGNCC 6 cut(s) 264, 765, 1393, 1595, 2331, 2582
PspPPI RGGWCCY 2 cut(s) 1393, 2331
PspXI VCTCGAGB 1 cut(s) 288
PsrI GAACNNNNNNTAC 2 cut(s) 1725, 1757
PstNI CAGNNNCTG 4 cut(s) 1123, 1220, 2348, 2483
PsuI RGATCY 1 cut(s) 2821
RsaI GTAC 8 cut(s) 122, 959, 1563, 1796, 2176, 2365, 2840, 3041
RsaNI GTAC 8 cut(s) 121, 958, 1562, 1795, 2175, 2364, 2839, 3040
RseI CAYNNNNRTG 3 cut(s) 1308, 2226, 2972
SacII CCGCGG 1 cut(s) 771
Sau3AI GATC 8 cut(s) 1099, 1176, 1875, 2531, 2668, 2821, 2874, 2964
Sau96I GGNCC 6 cut(s) 264, 765, 1393, 1595, 2331, 2582
SchI GAGTC 6 cut(s) 197, 227, 1616, 2176, 2489, 2597
ScrFI CCNGG 2 cut(s) 1937, 2705
SduI GDGCHC 6 cut(s) 1214, 1404, 1908, 2328, 2624, 2945
SfaNI GCATC 5 cut(s) 1605, 2249, 2335, 2504, 2887
SfcI CTRYAG 1 cut(s) 3109
Sfr274I CTCGAG 1 cut(s) 288
Sfr303I CCGCGG 1 cut(s) 771
SgrBI CCGCGG 1 cut(s) 771
SinI GGWCC 5 cut(s) 264, 1393, 1595, 2331, 2582
SlaI CTCGAG 1 cut(s) 288
SmiMI CAYNNNNRTG 3 cut(s) 1308, 2226, 2972
SmlI CTYRAG 3 cut(s) 288, 929, 2617
SmoI CTYRAG 3 cut(s) 288, 929, 2617
SpeI ACTAGT 1 cut(s) 3011
SphI GCATGC 1 cut(s) 1358
SspI AATATT 3 cut(s) 494, 1274, 2761
SspMI CTAG 1 cut(s) 3012
StyD4I CCNGG 2 cut(s) 1935, 2703
StyI CCWWGG 2 cut(s) 1624, 2576
TaiI ACGT 5 cut(s) 589, 2048, 2755, 2812, 3041
TatI WGTACW 1 cut(s) 957
TauI GCSGC 6 cut(s) 16, 165, 301, 651, 706, 2557
TscAI CASTG 7 cut(s) 408, 813, 1472, 1710, 2038, 2276, 2613
TseFI GTSAC 3 cut(s) 1364, 1501, 3068
TseI GCWGC 8 cut(s) 159, 200, 229, 816, 1031, 1765, 2384, 2507
Tsp45I GTSAC 3 cut(s) 1364, 1501, 3068
TspGWI ACGGA 1 cut(s) 903
TspRI CASTG 7 cut(s) 408, 813, 1472, 1710, 2038, 2276, 2613
VneI GTGCAC 3 cut(s) 1904, 2324, 2941
VpaK11BI GGWCC 5 cut(s) 264, 1393, 1595, 2331, 2582
XagI CCTNNNNNAGG 1 cut(s) 2010
XapI RAATTY 8 cut(s) 37, 223, 568, 595, 1371, 2536, 2649, 3116
XceI RCATGY 3 cut(s) 1358, 1968, 2205
XcmI CCANNNNNNNNNTGG 3 cut(s) 1310, 1916, 2282
XhoI CTCGAG 1 cut(s) 288
XmnI GAANNNNTTC 2 cut(s) 485, 2414
XspI CTAG 1 cut(s) 3012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.