RLG00000027874
ERF Family

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX MLL subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0003180
Physical Location & Seq
Forward (+)
16298786 .. 16306596
7811 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027874

Sequence Viewer

Length: 3129 bp
ATGATAATTAAGCGGCCGTCGAGATTTGAAATGCCGAATTTGAAACGGTGTAAGCTGGAGGAGGCGGAAGCTAAAGCCTATGCGTATTCGGCGAATCCGAAGAGGCGGAAAGCGAATGGGTACTGCTCAATTGGCAATGGCGAGGGGGAGGATTTGAGCAGCGGCTCCGGTTCGTGGGGATACGACGAGTCGTTTTCGGCTGCGGTCGAGGTGGTGGAGTCGAATTTGCAGCGGTTGAGTAACCCGCGAGAAATGAATCGGAGGTCTGAGAAGTTGAGGAGGTCAGCTCGAGGACGGCCGCAAGTGCCGTCTTCGAGGTTTAATGGTTCAGGTGATTTATGTGAGAAAAAGCAAGCGAAATATGTAGATACATGTACGCTTTATGAAGAAGAAGAATGTGATGTTTTAGCAGTGGAAGACAGAGAAGGGATTGAGAAATTGGGTTTTATTAAAAAGCGTAAGGAGGATATCAATGGGTTGAAGAAGTTCCAATATTGTGGGAAGGTAGAGCAAGAAGAAGTGGATTATTATGATGATATTGGTTACAGACAGTATATGGGTTTGAAAAATTCGATAGCGTCGAAACGTGGTGGAAATTCTTTGCCAGAGGAAAGAAGTGAGGAATATGGAGCTGGGTTTAGTTTTGGCGGCAAGGGGAAAGTTAGCAAACCCGATTGTGCAAAGAAGAAAGAAGTGTATAAGCCGCAGGACTTTGCTTTGGGTGATATTGTTTGGGCTAAGTGTGGGAGGAGCTACCCAGTTTGGCCGGCGGTGGTTATCGACCCCATACTGCAAGCTCCAGAAGCAGTGTTGAGCTGCTGTATTCCTAATGCTATCTGTGTCATGTTCTTTGGGTTCTCAAAGAATGGAACGCAAAGGGATTATGGGTGGGTGAAACAGGGAATGATATATCCGTTCAAGCAGTTCCTTGAGAGATTTCGAGGACAGACCCAGTTGTACAGGAGTAAACCAAGTGAGCTTCAGATGGCAATAGAGGAGGCGGTGTTAGCAGAAAATGGCTTTATTTACGCAGCTTGTAAAACTACTGATTCAGAGGCAAATGCCCCTGAAATCCAAGAGGCCACAGATTTTTATCAAGATCAGGCATATTACAGTCAGAATCTGAATCAGGAAGCATATCAGAAGGACACAAGACCTTGTGATTGCTGTGGTTTGATCACTCCATCTAAAGCTATGAAAAAAGTGAAGGGCTCAGGTTCTGAAACCCAAGTTTTATGTAAACACTGTGCAAAGTTGAAAAAATCAAGACAATATTGTGGCATATGTAAGAAGATATGGCACCATTCAGATGGTGGAGATTGGGTATGCTGTGATGGCTGTAATGTTTGGGTGCATGCTGAGTGTGACAAAATTTCCAGTAAAGTTTTCAAGGACCCAGAGCACATTGATTACTATTGTCCTGATTGCAAAAATAAGGCCAAAGGTGAAATGTTAGATGAGTTACAGTGTCAGCGAAAACACAGTTCCGGCCGGAAATCTGTGACTGGCGTAACTGTGACTGAGGTAACTGTGGTTTGTAATGGCATGGAAGGAACTTATGTACCAAACCTTCATATGGTTGCTTGTAAGTGTGGACCCTGTGATGAGTCAAAGATGCAGAGCCTTGGTGAATGGGAAAGGCACACAGGTTGTAGAGCAAAAAAATGGAAGCATAGTGTGAAAGTGAAGGCCACAATGCTAACACTGGAACAATGGATTGCAGAGTATAATGAACATGGCTTACAGCCTCTCAAATTAGATAAGCAGCGACTACTTGATTCCTTACAAGAAAAGTACGAGCCAGTTTATGCAAAGTGGACAAGTGAAAGATGTGCAGTTTGTAGATGGGTTGAAGATTGGGAAGATAACAAAATGATCATATGCAACAGGTGTCAAATAGCTGTGCACCAAGAATGCTATGGTGCAAAAGATGTCCAGGATTTTACTTCATGGGTTTGTAGAGCATGTGAAACACCTGATATCATTAAAGAATGTTGTCTCTGTCCTGTAAAAGGAGGTGCTTTGAAGCCCACTGATGTTGACACCTTGTGGGTTCATGTCTCTTGTGCTTGGTTTCGTCCCGAAATAGGTTTCTTGAATCATGAGAAGATGGAACCTGCTGTTGGAATCCTTAGAATCCCACCCATTACTTTTATGAAGCGTTGTGTAATTTGTACGCAGACTCATGGTTCCTGCACCACATGTTGCAAATGTGCCACTCATTTTCATGCAACCTGTGCATCAAGAGCAGGCTACAACATGGAATTGCACTGTCAAGAGAAGAATGGGAAACAAGTAACAACGAAGTTGATTTATTGTGCGGTGCACAGGACCCCTAATCCAGATGCTGTTGTCGTTGTTCATACTCCTTCAGGGGTTTTTGCTGCCAGAAGCTTGCTTCAAAACGAGAAAGGGTTCTTCAGGGGTTCGAGGGTTGTCTCATCAAAGAGGACTGAAGTTCCGGAACCTTCAACCTCAGAGTCTGATGAGTTAGAACCACTCTCTGCTGCAAGATGCCGTGCTATTAAAAGATCAAATTTTAAGGGAGGTGAGCGGCTACCAATTTTTCATCGACCAAGGGGATCAACACGTCATTCATTAGACTCAGTGAATAGCTTGAGCACATTTATAGAAGTAGAAGATTCTAAAATTTTTTCTTCCTTCAAGGATCGGCTTTACCACTTGCAGAAAACTGAAAATCACCGGGTTTGTTTTGGTAAATCTGGAATACATGGATGGGGTCTATTTGCACGGAGGAATATTCAAGAAGGAGAAATGGTTGTTGAGTATCGTGGTGAGCGGGTAAGACGTAGTGTTGCAGATCTTAGGGAAGCACAGTACAGGCTACAAGGCAAAGATTGCTATCTTTTCAAGATCAGCGAGGAAGTAGTAATTGATGCTACATATAGAGGAAATATAGCACGGTTAATTAACCATTCGTGCACGCCCAATTGCTATTCAAGGATCATGAGTGTGGGTGAGGAGAACCGAATAGTTCTCATTGCCAAGACTAGTGTTTCGGCTGGTGAAGAACTAACGTACGATTACTTGTTTGAGCCGGATGAATGTGACGAGCTGAGAGTTCCCTGTCTATGTAAAGCTCCCAACTGTAGGAAATTCTTGTGTTAG

Protein Analysis

1043

Amino Acids

118.15

Weight (kDa)

8.39

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 241 - 336 7.3e-15 PWWP domain
PHD PF00628 426 - 476 6.7e-07 PHD-finger
PHD PF00628 611 - 656 9.5e-06 PHD-finger
PHD_2 PF13831 623 - 657 1.4e-12 PHD-finger
zf-HC5HC2H_2 PF13832 664 - 776 1.4e-25 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 686 - 777 2.9e-10 PHD-like zinc-binding domain
SET PF00856 912 - 1016 6.5e-27 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2125
AccB1I GGYRCC 1 cut(s) 1299
AccBSI CCGCTC 2 cut(s) 2554, 2800
AccII CGCG 1 cut(s) 247
AccIII TCCGGA 1 cut(s) 2461
AclWI GGATC 3 cut(s) 2590, 2676, 2972
AcoI YGGCCR 4 cut(s) 14, 296, 764, 1489
AcsI RAATTY 8 cut(s) 37, 223, 568, 595, 1371, 2536, 2649, 3116
AcuI CTGAAG 4 cut(s) 965, 2355, 2404, 2475
AdeI CACNNNGTG 1 cut(s) 2049
AfaI GTAC 8 cut(s) 122, 376, 959, 1563, 1796, 2176, 2840, 3041
AfiI CCNNNNNNNGG 6 cut(s) 174, 1576, 2012, 2087, 2123, 3111
AflIII ACRYGT 3 cut(s) 371, 2201, 2588
AhlI ACTAGT 1 cut(s) 3011
AjiI CACGTC 1 cut(s) 2591
AjnI CCWGG 1 cut(s) 1935
AjuI GAANNNNNNNTTGG 2 cut(s) 2106, 2138
Alw21I GWGCWC 5 cut(s) 1404, 1908, 2328, 2624, 2945
Alw26I GTCTC 3 cut(s) 2003, 2065, 2443
Alw44I GTGCAC 3 cut(s) 1904, 2324, 2941
AlwI GGATC 3 cut(s) 2590, 2676, 2972
AlwNI CAGNNNCTG 4 cut(s) 1123, 1220, 2348, 2483
Ama87I CYCGRG 1 cut(s) 288
Aor13HI TCCGGA 1 cut(s) 2461
AoxI GGCC 7 cut(s) 14, 296, 764, 1080, 1437, 1489, 1689
ApaLI GTGCAC 3 cut(s) 1904, 2324, 2941
ApeKI GCWGC 8 cut(s) 159, 200, 229, 816, 1031, 1765, 2384, 2507
ApoI RAATTY 8 cut(s) 37, 223, 568, 595, 1371, 2536, 2649, 3116
ArsI GACNNNNNNTTYG 2 cut(s) 3001, 3033
Asp700I GAANNNNTTC 2 cut(s) 485, 2414
AspS9I GGNCC 3 cut(s) 1393, 1595, 2331
AsuC2I CCSGG 1 cut(s) 2705
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 3 cut(s) 1393, 1595, 2331
BaeGI GKGCMC 3 cut(s) 1908, 2328, 2945
BaeI ACNNNNGTAYC 2 cut(s) 1545, 1578
BanI GGYRCC 1 cut(s) 1299
BanII GRGCYC 1 cut(s) 1214
BarI GAAGNNNNNNTAC 2 cut(s) 1554, 1586
BbsI GAAGAC 2 cut(s) 303, 423
Bbv12I GWGCWC 5 cut(s) 1404, 1908, 2328, 2624, 2945
BbvI GCAGC 8 cut(s) 171, 187, 241, 803, 1043, 1777, 2371, 2494
BccI CCATC 7 cut(s) 979, 1192, 1304, 1328, 1839, 2104, 2730
BceAI ACGGC 3 cut(s) 292, 311, 2502
BciT130I CCWGG 1 cut(s) 1937
BciVI GTATCC 1 cut(s) 173
BclI TGATCA 2 cut(s) 1176, 1875
BcnI CCSGG 1 cut(s) 2705
BcoDI GTCTC 3 cut(s) 2003, 2065, 2443
BcuI ACTAGT 1 cut(s) 3011
BfaI CTAG 1 cut(s) 3012
BfmI CTRYAG 1 cut(s) 3109
BfuAI ACCTGC 1 cut(s) 2125
BfuI GTATCC 1 cut(s) 173
BglII AGATCT 1 cut(s) 2821
Bme1390I CCNGG 2 cut(s) 1937, 2705
Bme18I GGWCC 3 cut(s) 1393, 1595, 2331
BmeT110I CYCGRG 1 cut(s) 288
BmgBI CACGTC 1 cut(s) 2591
BmgT120I GGNCC 3 cut(s) 1393, 1595, 2331
BmiI GGNNCC 8 cut(s) 166, 1301, 1395, 1597, 2115, 2191, 2333, 2466
BmrFI CCNGG 2 cut(s) 1937, 2705
BmrI ACTGGG 2 cut(s) 752, 946
BmsI GCATC 5 cut(s) 1605, 2249, 2335, 2504, 2887
BmuI ACTGGG 2 cut(s) 752, 946
BpiI GAAGAC 2 cut(s) 303, 423
BplI GAGNNNNNCTC 2 cut(s) 271, 303
BpmI CTGGAG 2 cut(s) 77, 783
Bpu10I CCTNAGC 1 cut(s) 1213
BpuEI CTTGAG 2 cut(s) 950, 2638
BpuMI CCSGG 1 cut(s) 2705
BsaBI GATNNNNATC 2 cut(s) 1092, 2862
BsaJI CCNNGG 2 cut(s) 1624, 2576
BsaWI WCCGGW 2 cut(s) 167, 2461
BsaXI ACNNNNNCTCC 6 cut(s) 989, 1019, 1308, 1338, 2763, 2793
Bsc4I CCNNNNNNNGG 6 cut(s) 174, 1576, 2012, 2087, 2123, 3111
Bse118I RCCGGY 1 cut(s) 766
Bse1I ACTGG 6 cut(s) 758, 952, 1377, 1510, 1710, 1802
Bse3DI GCAATG 2 cut(s) 142, 3000
Bse8I GATNNNNATC 2 cut(s) 1092, 2862
BseAI TCCGGA 1 cut(s) 2461
BseBI CCWGG 1 cut(s) 1937
BseDI CCNNGG 2 cut(s) 1624, 2576
BseGI GGATG 2 cut(s) 2741, 3067
BseJI GATNNNNATC 2 cut(s) 1092, 2862
BseLI CCNNNNNNNGG 6 cut(s) 174, 1576, 2012, 2087, 2123, 3111
BseMI GCAATG 2 cut(s) 142, 3000
BseMII CTCAG 7 cut(s) 258, 1227, 1350, 1512, 2490, 2619, 3068
BseNI ACTGG 6 cut(s) 758, 952, 1377, 1510, 1710, 1802
BseRI GAGGAG 5 cut(s) 74, 292, 763, 1010, 2996
BseSI GKGCMC 3 cut(s) 1908, 2328, 2945
BseX3I CGGCCG 3 cut(s) 14, 296, 1489
BseXI GCAGC 8 cut(s) 171, 187, 241, 803, 1043, 1777, 2371, 2494
BseYI CCCAGC 1 cut(s) 632
BsgI GTGCAG 2 cut(s) 1854, 2179
Bsh1236I CGCG 1 cut(s) 247
Bsh1285I CGRYCG 4 cut(s) 17, 207, 299, 1492
BshFI GGCC 7 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691
BshNI GGYRCC 1 cut(s) 1299
BsiEI CGRYCG 4 cut(s) 17, 207, 299, 1492
BsiHKAI GWGCWC 5 cut(s) 1404, 1908, 2328, 2624, 2945
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 7 cut(s) 168, 767, 1488, 1492, 2462, 2704, 3059
BsiWI CGTACG 1 cut(s) 3039
BslFI GGGAC 1 cut(s) 2064
BslI CCNNNNNNNGG 6 cut(s) 174, 1576, 2012, 2087, 2123, 3111
BsmAI GTCTC 3 cut(s) 2003, 2065, 2443
BsmFI GGGAC 1 cut(s) 2064
BsmI GAATGC 1 cut(s) 1919
BsnI GGCC 7 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 6 cut(s) 1214, 1404, 1908, 2328, 2624, 2945
Bsp13I TCCGGA 1 cut(s) 2461
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 9 cut(s) 1099, 1176, 1875, 2531, 2582, 2668, 2821, 2874, 2964
BspANI GGCC 7 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691
BspCNI CTCAG 7 cut(s) 259, 1226, 1351, 1513, 2489, 2618, 3069
BspEI TCCGGA 1 cut(s) 2461
BspFNI CGCG 1 cut(s) 247
BspHI TCATGA 2 cut(s) 2101, 2967
BspLI GGNNCC 8 cut(s) 166, 1301, 1395, 1597, 2115, 2191, 2333, 2466
BspMI ACCTGC 1 cut(s) 2125
BspPI GGATC 3 cut(s) 2590, 2676, 2972
BspT107I GGYRCC 1 cut(s) 1299
BsrBI CCGCTC 2 cut(s) 2554, 2800
BsrDI GCAATG 2 cut(s) 142, 3000
BsrFI RCCGGY 1 cut(s) 766
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 6 cut(s) 758, 952, 1377, 1510, 1710, 1802
BssAI RCCGGY 1 cut(s) 766
BssECI CCNNGG 2 cut(s) 1624, 2576
BssMI GATC 9 cut(s) 1099, 1176, 1875, 2531, 2582, 2668, 2821, 2874, 2964
BssT1I CCWWGG 2 cut(s) 1624, 2576
Bst2UI CCWGG 1 cut(s) 1937
Bst6I CTCTTC 1 cut(s) 95
BstAPI GCANNNNNTGC 2 cut(s) 2237, 2859
BstAUI TGTACA 1 cut(s) 957
BstC8I GCNNGC 7 cut(s) 354, 768, 795, 1356, 2251, 2396, 2945
BstENI CCTNNNNNAGG 1 cut(s) 2010
BstF5I GGATG 2 cut(s) 2741, 3067
BstFNI CGCG 1 cut(s) 247
BstKTI GATC 9 cut(s) 1102, 1179, 1878, 2534, 2585, 2671, 2824, 2877, 2967
BstMAI GTCTC 3 cut(s) 2003, 2065, 2443
BstMBI GATC 9 cut(s) 1099, 1176, 1875, 2531, 2582, 2668, 2821, 2874, 2964
BstMCI CGRYCG 4 cut(s) 17, 207, 299, 1492
BstMWI GCNNNNNNNGC 9 cut(s) 89, 132, 304, 803, 1007, 1335, 2237, 2246, 2859
BstNI CCWGG 1 cut(s) 1937
BstNSI RCATGY 4 cut(s) 375, 1358, 1968, 2205
BstSCI CCNGG 2 cut(s) 1935, 2703
BstSFI CTRYAG 1 cut(s) 3109
BstSLI GKGCMC 3 cut(s) 1908, 2328, 2945
BstUI CGCG 1 cut(s) 247
BstV1I GCAGC 8 cut(s) 171, 187, 241, 803, 1043, 1777, 2371, 2494
BstV2I GAAGAC 2 cut(s) 303, 423
BstX2I RGATCY 1 cut(s) 2821
BstXI CCANNNNNNTGG 2 cut(s) 497, 1310
BstYI RGATCY 1 cut(s) 2821
BstZI CGGCCG 3 cut(s) 14, 296, 1489
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 7 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691
BtrI CACGTC 1 cut(s) 2591
BtsCI GGATG 2 cut(s) 2741, 3067
BtsI GCAGTG 2 cut(s) 417, 813
BtsIMutI CAGTG 8 cut(s) 417, 813, 1243, 1472, 1703, 2031, 2269, 2613
BveI ACCTGC 1 cut(s) 2125
Cac8I GCNNGC 7 cut(s) 354, 768, 795, 1356, 2251, 2396, 2945
CaiI CAGNNNCTG 4 cut(s) 1123, 1220, 2348, 2483
CciI TCATGA 2 cut(s) 2101, 2967
Cfr10I RCCGGY 1 cut(s) 766
Cfr13I GGNCC 3 cut(s) 1393, 1595, 2331
CseI GACGC 1 cut(s) 567
Csp6I GTAC 8 cut(s) 121, 375, 958, 1562, 1795, 2175, 2839, 3040
CspCI CAANNNNNGTGG 2 cut(s) 2020, 2055
CviQI GTAC 8 cut(s) 121, 375, 958, 1562, 1795, 2175, 2839, 3040
DpnI GATC 9 cut(s) 1101, 1178, 1877, 2533, 2584, 2670, 2823, 2876, 2966
DpnII GATC 9 cut(s) 1099, 1176, 1875, 2531, 2582, 2668, 2821, 2874, 2964
DraIII CACNNNGTG 1 cut(s) 2049
EaeI YGGCCR 4 cut(s) 14, 296, 764, 1489
EagI CGGCCG 3 cut(s) 14, 296, 1489
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
EciI GGCGGA 2 cut(s) 80, 121
EclXI CGGCCG 3 cut(s) 14, 296, 1489
Eco130I CCWWGG 2 cut(s) 1624, 2576
Eco24I GRGCYC 1 cut(s) 1214
Eco32I GATATC 2 cut(s) 469, 1981
Eco47I GGWCC 3 cut(s) 1393, 1595, 2331
Eco52I CGGCCG 3 cut(s) 14, 296, 1489
Eco57I CTGAAG 4 cut(s) 965, 2355, 2404, 2475
Eco88I CYCGRG 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 2010
EcoO109I RGGNCCY 2 cut(s) 1393, 2331
EcoRII CCWGG 1 cut(s) 1935
EcoRV GATATC 2 cut(s) 469, 1981
EcoT14I CCWWGG 2 cut(s) 1624, 2576
EcoT38I GRGCYC 1 cut(s) 1214
ErhI CCWWGG 2 cut(s) 1624, 2576
FaqI GGGAC 1 cut(s) 2064
FauI CCCGC 2 cut(s) 252, 2793
FauNDI CATATG 3 cut(s) 1283, 1575, 1880
FbaI TGATCA 2 cut(s) 1176, 1875
FokI GGATG 2 cut(s) 2748, 3074
FriOI GRGCYC 1 cut(s) 1214
FspBI CTAG 1 cut(s) 3012
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 2 cut(s) 77, 783
HaeIII GGCC 7 cut(s) 16, 298, 766, 1082, 1439, 1491, 1691
HapII CCGG 7 cut(s) 168, 767, 1488, 1492, 2462, 2704, 3059
HgaI GACGC 1 cut(s) 567
HincII GTYRAC 1 cut(s) 2041
HindII GTYRAC 1 cut(s) 2041
HindIII AAGCTT 1 cut(s) 2392
HpaII CCGG 7 cut(s) 168, 767, 1488, 1492, 2462, 2704, 3059
Hpy166II GTNNAC 8 cut(s) 968, 1241, 1595, 1818, 1906, 2041, 2326, 2943
Hpy8I GTNNAC 8 cut(s) 968, 1241, 1595, 1818, 1906, 2041, 2326, 2943
Hpy99I CGWCG 3 cut(s) 22, 188, 583
HpyCH4IV ACGT 4 cut(s) 586, 2590, 2809, 3038
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 132, 304, 803, 1007, 1335, 2237, 2246, 2859
HpySE526I ACGT 4 cut(s) 586, 2590, 2809, 3038
Kpn2I TCCGGA 1 cut(s) 2461
KroI GCCGGC 1 cut(s) 766
KroNI GCCGGC 1 cut(s) 768
Ksp22I TGATCA 2 cut(s) 1176, 1875
Kzo9I GATC 9 cut(s) 1099, 1176, 1875, 2531, 2582, 2668, 2821, 2874, 2964
LmnI GCTCC 5 cut(s) 170, 629, 750, 802, 3106
Lsp1109I GCAGC 8 cut(s) 171, 187, 241, 803, 1043, 1777, 2371, 2494
LweI GCATC 5 cut(s) 1605, 2249, 2335, 2504, 2887
MaeI CTAG 1 cut(s) 3012
MaeII ACGT 4 cut(s) 586, 2590, 2809, 3038
MalI GATC 9 cut(s) 1101, 1178, 1877, 2533, 2584, 2670, 2823, 2876, 2966
MbiI CCGCTC 2 cut(s) 2554, 2800
MboI GATC 9 cut(s) 1099, 1176, 1875, 2531, 2582, 2668, 2821, 2874, 2964
MfeI CAATTG 2 cut(s) 129, 2950
MflI RGATCY 1 cut(s) 2821
MhlI GDGCHC 6 cut(s) 1214, 1404, 1908, 2328, 2624, 2945
MlyI GAGTC 6 cut(s) 197, 227, 1616, 2176, 2489, 2597
MmeI TCCRAC 1 cut(s) 2104
MroI TCCGGA 1 cut(s) 2461
MroNI GCCGGC 1 cut(s) 766
MroXI GAANNNNTTC 2 cut(s) 485, 2414
MseI TTAA 8 cut(s) 9, 321, 450, 1986, 2526, 2541, 2927, 2931
MslI CAYNNNNRTG 3 cut(s) 1308, 2226, 2972
MspA1I CMGCKG 2 cut(s) 162, 232
MspI CCGG 7 cut(s) 168, 767, 1488, 1492, 2462, 2704, 3059
MspR9I CCNGG 2 cut(s) 1937, 2705
MunI CAATTG 2 cut(s) 129, 2950
Mva1269I GAATGC 1 cut(s) 1919
MvaI CCWGG 1 cut(s) 1937
MvnI CGCG 1 cut(s) 247
MwoI GCNNNNNNNGC 9 cut(s) 89, 132, 304, 803, 1007, 1335, 2237, 2246, 2859
NaeI GCCGGC 1 cut(s) 768
NciI CCSGG 1 cut(s) 2705
NdeI CATATG 3 cut(s) 1283, 1575, 1880
NdeII GATC 9 cut(s) 1099, 1176, 1875, 2531, 2582, 2668, 2821, 2874, 2964
NgoMIV GCCGGC 1 cut(s) 766
NlaIV GGNNCC 8 cut(s) 166, 1301, 1395, 1597, 2115, 2191, 2333, 2466
NmuCI GTSAC 4 cut(s) 1364, 1501, 1516, 3068
NspI RCATGY 4 cut(s) 375, 1358, 1968, 2205
PacI TTAATTAA 1 cut(s) 2931
PaeI GCATGC 1 cut(s) 1358
PaeR7I CTCGAG 1 cut(s) 288
PagI TCATGA 2 cut(s) 2101, 2967
PciI ACATGT 2 cut(s) 371, 2201
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 578
PctI GAATGC 1 cut(s) 1919
PdiI GCCGGC 1 cut(s) 768
PdmI GAANNNNTTC 2 cut(s) 485, 2414
Pfl23II CGTACG 1 cut(s) 3039
PfoI TCCNGGA 1 cut(s) 1935
PleI GAGTC 6 cut(s) 196, 226, 1615, 2176, 2488, 2597
PpsI GAGTC 6 cut(s) 196, 226, 1615, 2176, 2488, 2597
PpuMI RGGWCCY 2 cut(s) 1393, 2331
PscI ACATGT 2 cut(s) 371, 2201
Psp5II RGGWCCY 2 cut(s) 1393, 2331
Psp6I CCWGG 1 cut(s) 1935
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 1 cut(s) 1935
PspLI CGTACG 1 cut(s) 3039
PspN4I GGNNCC 8 cut(s) 166, 1301, 1395, 1597, 2115, 2191, 2333, 2466
PspPI GGNCC 3 cut(s) 1393, 1595, 2331
PspPPI RGGWCCY 2 cut(s) 1393, 2331
PspXI VCTCGAGB 1 cut(s) 288
PsrI GAACNNNNNNTAC 2 cut(s) 1725, 1757
PstNI CAGNNNCTG 4 cut(s) 1123, 1220, 2348, 2483
PsuI RGATCY 1 cut(s) 2821
RsaI GTAC 8 cut(s) 122, 376, 959, 1563, 1796, 2176, 2840, 3041
RsaNI GTAC 8 cut(s) 121, 375, 958, 1562, 1795, 2175, 2839, 3040
RseI CAYNNNNRTG 3 cut(s) 1308, 2226, 2972
SaqAI TTAA 8 cut(s) 9, 321, 450, 1986, 2526, 2541, 2927, 2931
Sau3AI GATC 9 cut(s) 1099, 1176, 1875, 2531, 2582, 2668, 2821, 2874, 2964
Sau96I GGNCC 3 cut(s) 1393, 1595, 2331
SchI GAGTC 6 cut(s) 197, 227, 1616, 2176, 2489, 2597
ScrFI CCNGG 2 cut(s) 1937, 2705
SduI GDGCHC 6 cut(s) 1214, 1404, 1908, 2328, 2624, 2945
SfaNI GCATC 5 cut(s) 1605, 2249, 2335, 2504, 2887
SfcI CTRYAG 1 cut(s) 3109
Sfr274I CTCGAG 1 cut(s) 288
SinI GGWCC 3 cut(s) 1393, 1595, 2331
SlaI CTCGAG 1 cut(s) 288
SmiMI CAYNNNNRTG 3 cut(s) 1308, 2226, 2972
SmlI CTYRAG 3 cut(s) 288, 929, 2617
SmoI CTYRAG 3 cut(s) 288, 929, 2617
SpeI ACTAGT 1 cut(s) 3011
SphI GCATGC 1 cut(s) 1358
SspI AATATT 3 cut(s) 494, 1274, 2761
SspMI CTAG 1 cut(s) 3012
StyD4I CCNGG 2 cut(s) 1935, 2703
StyI CCWWGG 2 cut(s) 1624, 2576
TaiI ACGT 4 cut(s) 589, 2593, 2812, 3041
TatI WGTACW 2 cut(s) 957, 2838
TauI GCSGC 6 cut(s) 16, 165, 301, 651, 706, 2557
Tru1I TTAA 8 cut(s) 9, 321, 450, 1986, 2526, 2541, 2927, 2931
Tru9I TTAA 8 cut(s) 9, 321, 450, 1986, 2526, 2541, 2927, 2931
TscAI CASTG 8 cut(s) 417, 813, 1250, 1472, 1710, 2038, 2276, 2613
TseFI GTSAC 4 cut(s) 1364, 1501, 1516, 3068
TseI GCWGC 8 cut(s) 159, 200, 229, 816, 1031, 1765, 2384, 2507
Tsp45I GTSAC 4 cut(s) 1364, 1501, 1516, 3068
TspGWI ACGGA 2 cut(s) 903, 2767
TspRI CASTG 8 cut(s) 417, 813, 1250, 1472, 1710, 2038, 2276, 2613
VneI GTGCAC 3 cut(s) 1904, 2324, 2941
VpaK11BI GGWCC 3 cut(s) 1393, 1595, 2331
XagI CCTNNNNNAGG 1 cut(s) 2010
XapI RAATTY 8 cut(s) 37, 223, 568, 595, 1371, 2536, 2649, 3116
XceI RCATGY 4 cut(s) 375, 1358, 1968, 2205
XcmI CCANNNNNNNNNTGG 2 cut(s) 1310, 1916
XhoI CTCGAG 1 cut(s) 288
XmnI GAANNNNTTC 2 cut(s) 485, 2414
XspI CTAG 1 cut(s) 3012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.