Rh1CG268200
ERF Family

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX MLL subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0003180
Physical Location & Seq
Reverse (-)
53297811 .. 53306109
8299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG268200.1

Sequence Viewer

Length: 3186 bp
ATGATAATTAAGCGGCGGTCGAGATTTGAAATGCCGAATTTGAAACGGTGTAAGCTGGAGGAGGCGGAAGCTAAAGCCTATGCGTATTCGGCGAATCCGAAGAGGCGGAAAGCGAATGGGTACTGCTCAGTTGGCAATGGCGAGGGGGAGGATTTGAGCAGCGGCTCCGGTTCGTCGGGATACGACGAGTCGTTTTCGGCTGCGGTCGAGGTGGTGGAGTCGAATTTGAGTAAGCGGCGAGAAATGAATCGGAGGTCCGAGAAGTTGAGGAGGTCTGCTCGAGGACGGCCGCAAGTGCCGTCTTCGAGGTTTAACGGTTCAGGTGAGAAAAAGCAAGCGAAATATGTAGATACATATACGGTTTTTGAAGTTGAAGAAGAATGTGATGTTTTAGCAGTGGAGGACAGAGAAGGGATTGAGAAATTGGGTTTTGTTAAAAAGCGAAAGGAGGATATCAATGGGTTTAAGAAGTCGAAGAAGTTTCAATATTGTGGGAAGGTAGAGCAAGAAGAAGTGGATTATTATGATGATATTGGTTACAGACAGTATATGGGTTTGAAAAATTCGATAGCGTCGAAACGTGGTGGAAATTCTTTGCCAGAGGAAAGAAGTGAGGAATATGGAGCTGGGTTTAGTTTTGGCGGCAAGGAGAAAGTTCGCAAACCCGATTGTGCAAAGAAGAAAGAAGTGTATAAGCCGCAGGACTTTGCTTTGGGTGATATTGTTTGGGCCAAGTGTGGGAGGAGCTACCCAGTTTGGCCGGCGGTGGTTATCGACCCCATATTGCAAGCTCCAGAAGCAGTGTTGAGCTGCTGTATTCCTAATGCAATCTGTGTCATGTTCTTTGGGTTCTCAAAGAATGGAACACAAAGGGATTATGGGTGGGTGAAGCAGGGAATGATATATCCGTTCAAGCAGTTCCTTGAGAGATTTCGAGGACAGACCCAGTTGTACAGGAGTAAACCAAGCGAGCTTCAGATGGCAATAGAGGAGGCAGTGTTAGCAGAAAATGGTTTTATTTACGCAGCTTGTAAAACTACTGATTCAGAGGCAAATGCCCCTGAAATCCAAGAGGCCACAGATTTTTATCAAGATCAGGCATATTACAGTCAGAATCTGAATCAGGAAGCATATCAGAAGGACACAAGACCTTGTGATTGCTGTGGTTTGATCACTCCTTCTAAAGCTATGAAAAAAGTGAAGGGCTCAGGTTCTGAAACCCAAGTTTTATGTAAACATTGTGCAAAGTTGAAAAAATCAAGACAATATTGTGGCATATGTAAGAAGATATGGCACCATTCAGATGGTGGAGATTGGGTATGCTGTGATGGCTGTAATGTTTGGGTGCATGCTGAGTGTGACAAAATTTCCAGTAAAGTTTTCAAGGACCCAGAGCACATTGATTACTATTGTCCTGATTGCAAAAATAAGGCCAAAGGTGAAATGTTAGATGAGTTACAGTGTCGGCAAAAACACAGTTCTGGCCGGAAATCTGTGACTGGCATAACTGTGTCTGAGGTAACTGTGGTTTGTAATGGCATGGAAGGAACTTATGTACCAAACCTTCATATGGTTGCTTGTAAGTGTGGACCCTGTGATGAGTCAAAGATGCAGAGCCTTGGTGAATGGGAAAGGCACACAGGTTGTAGAGCAAAAAAATGGAAGCATAGTGTGAAAGTGAAGGCCACAATGCTAACACTGGAACAATGGATTGCAGAGTATAATGAACATGGCTTACAGCCTCTCAAATTAGATAAGCAGCGACTACTTGATTCCTTACAAGAAAAGTACGAGCCAGTTAATGCAAAGTGGACAAGTGAAAGATGTGCAGTTTGTAGATGGGTTGAAGATTGGGAAGATAACAAAATGATCATATGCAACAGGTGTCAAATAGCTGTGCACCAAGAATGCTATGGTGCAAAAGATGTCCAGGATTTTACTTCATGGGTTTGTAGAGCATGTGAAACACCTGATATCATTAAAGAATGTTGTCTCTGTCCTGTAAAAGGAGGTGCTTTGAAGCCCACTGATGTTGACACCTTGTGGGTTCATGTCTCTTGTGCTTGGTTTCGTCCTGAAGTAGGTTTCTTGAATCATGAGAAGATGGAACCTGCTGTTGGAATCCTTAGAATCCCACCCACTACCTTTATGAAGCGTTGCGTAATTTGTACGCAGACTCATGGTTCCTGCACCACATGTTGCAAATGTGCCACTCATTTTCATGCAACCTGTGCATCAAGAGCAGGCTACAACATGGAATTGCACTGCCAAGAGAAGAATGGGAAACAAGTAACAACGAAGATGATATATTGTGCTGTGCACAGGACCCCTAATCCAGATGCTGTTGTTGTTGTTCGTACTCCTTCGGGGGTTTTTGCTGCCAGAAGCTTGCTTCAAAACGAGAAAGGGTTCTTCAGGGGTTCGAGGGTGGTCTCATCAAAGAGGACTGAAGTTCCAGAACCTTCAACCTCAGAGTCTGATGAGTTAGAACCACTCTCTGCTGCAAGATGCCGTGCTATTAAAAGATCAAATTTTAAGGGAGGTGAGCGGCTACCAATTTTTCATCGACCAAGGGGACCAACACATCATTCATTAGACTCAGTGAATAGCTTGAGCACATTTATAGAAGTAGAAGATTCTAAAATTTTTTCTTCCTTCAAGGATCGGCTTTACCACTTGCAGAAAACTGAAAATCACCGGGTTTGTTTTGGTAAATCTGGAATACATGGATGGGGCCTATTTGCGCGTAGGAATATTCAAGAAGGAGAAATGGTTGTTGAGTATCGTGGTGAGCGGGTAAGACGTAGTGTTGCAGATCTGAGGGAAGCACGGTACAGGCTACAAGGCAAAGATTGCTATCTTTTCAAGATCAGCGAGGAAGTAGTAATTGATGCTACATATAGAGGAAATATAGCACGGTTAATTAACCATTCGTTGGTCTATTGGAAGTTCAGAATTACATATCATGATGCTAAAATTTTGTTGCACGTGCTGCAGTGCACACCCAATTGCTATTCAAGGATCATGAGTGTGGGTGAGGAGAACCGAATAGTTCTCATTGCCAAGACTAGTGTTTCGGCCGGTGAAGAACTAACGTACGATTACTTGTTTGAGCCGGATGAATGTGACGAGCTGAGAGTTCCCTGTCTATGTAAAGCTCCCAACTGTAGGAAATTCTTGTGTTAG

Protein Analysis

1061

Amino Acids

120.69

Weight (kDa)

8.63

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 239 - 334 7.5e-15 PWWP domain
PHD PF00628 424 - 474 6.8e-07 PHD-finger
PHD PF00628 609 - 654 9.7e-06 PHD-finger
PHD_2 PF13831 621 - 655 1.4e-12 PHD-finger
zf-HC5HC2H_2 PF13832 662 - 774 5.5e-26 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 684 - 775 1.2e-10 PHD-like zinc-binding domain
SET PF00856 910 - 1035 2.5e-21 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2119
AccB1I GGYRCC 1 cut(s) 1293
AccB7I CCANNNNNTGG 1 cut(s) 2935
AccBSI CCGCTC 2 cut(s) 2548, 2794
AccII CGCG 1 cut(s) 2746
AclWI GGATC 2 cut(s) 2670, 3029
AcoI YGGCCR 4 cut(s) 287, 758, 1483, 3078
AcsI RAATTY 9 cut(s) 37, 223, 562, 589, 1365, 2530, 2643, 2976, 3173
AcuI CTGAAG 4 cut(s) 959, 2097, 2398, 2469
AcvI CACGTG 1 cut(s) 2989
AdeI CACNNNGTG 1 cut(s) 2043
AfaI GTAC 8 cut(s) 122, 953, 1557, 1790, 2170, 2359, 2834, 3098
AfiI CCNNNNNNNGG 7 cut(s) 738, 1570, 2006, 2081, 2117, 2935, 3168
AflIII ACRYGT 1 cut(s) 2195
AhlI ACTAGT 1 cut(s) 3068
AjnI CCWGG 1 cut(s) 1929
AjuI GAANNNNNNNTTGG 4 cut(s) 2100, 2132, 2572, 2604
Alw21I GWGCWC 5 cut(s) 1398, 1902, 2322, 2618, 3002
Alw26I GTCTC 3 cut(s) 1997, 2059, 2437
Alw44I GTGCAC 3 cut(s) 1898, 2318, 2998
AlwI GGATC 2 cut(s) 2670, 3029
AlwNI CAGNNNCTG 4 cut(s) 1117, 1214, 2342, 2477
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 9 cut(s) 287, 729, 758, 1074, 1431, 1483, 1683, 2734, 3078
ApaLI GTGCAC 3 cut(s) 1898, 2318, 2998
ApeKI GCWGC 8 cut(s) 159, 200, 810, 1025, 1759, 2378, 2501, 2992
ApoI RAATTY 9 cut(s) 37, 223, 562, 589, 1365, 2530, 2643, 2976, 3173
ArsI GACNNNNNNTTYG 2 cut(s) 3058, 3090
Asp700I GAANNNNTTC 1 cut(s) 2408
AspLEI GCGC 1 cut(s) 2746
AspS9I GGNCC 7 cut(s) 255, 729, 1387, 1589, 2325, 2576, 2734
AsuC2I CCSGG 1 cut(s) 2699
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
BaeGI GKGCMC 3 cut(s) 1902, 2322, 3002
BaeI ACNNNNGTAYC 2 cut(s) 1539, 1572
BanI GGYRCC 1 cut(s) 1293
BanII GRGCYC 1 cut(s) 1208
BarI GAAGNNNNNNTAC 2 cut(s) 1548, 1580
BbrPI CACGTG 1 cut(s) 2989
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 5 cut(s) 1398, 1902, 2322, 2618, 3002
BbvI GCAGC 8 cut(s) 171, 187, 797, 1037, 1771, 2365, 2488, 2979
BccI CCATC 6 cut(s) 973, 1298, 1322, 1833, 2098, 2724
BceAI ACGGC 3 cut(s) 283, 302, 2496
BciT130I CCWGG 1 cut(s) 1931
BciVI GTATCC 1 cut(s) 173
BclI TGATCA 2 cut(s) 1170, 1869
BcnI CCSGG 1 cut(s) 2699
BcoDI GTCTC 3 cut(s) 1997, 2059, 2437
BcuI ACTAGT 1 cut(s) 3068
BfaI CTAG 1 cut(s) 3069
BfmI CTRYAG 2 cut(s) 2993, 3166
BfuAI ACCTGC 1 cut(s) 2119
BfuI GTATCC 1 cut(s) 173
BglII AGATCT 1 cut(s) 2815
Bme1390I CCNGG 2 cut(s) 1931, 2699
Bme18I GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 7 cut(s) 255, 729, 1387, 1589, 2325, 2576, 2734
BmiI GGNNCC 9 cut(s) 166, 1295, 1389, 1591, 2109, 2185, 2327, 2577, 2735
BmrFI CCNGG 2 cut(s) 1931, 2699
BmrI ACTGGG 2 cut(s) 746, 940
BmsI GCATC 6 cut(s) 1599, 2243, 2329, 2498, 2881, 2959
BmuI ACTGGG 2 cut(s) 746, 940
BpiI GAAGAC 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpmI CTGGAG 2 cut(s) 77, 777
Bpu10I CCTNAGC 1 cut(s) 1207
BpuEI CTTGAG 2 cut(s) 944, 2632
BpuMI CCSGG 1 cut(s) 2699
BsaAI YACGTR 1 cut(s) 2989
BsaBI GATNNNNATC 2 cut(s) 1086, 2856
BsaI GGTCTC 1 cut(s) 2437
BsaJI CCNNGG 2 cut(s) 1618, 2570
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 6 cut(s) 983, 1013, 1302, 1332, 2757, 2787
Bsc4I CCNNNNNNNGG 7 cut(s) 738, 1570, 2006, 2081, 2117, 2935, 3168
Bse118I RCCGGY 2 cut(s) 760, 3080
Bse1I ACTGG 6 cut(s) 752, 946, 1371, 1504, 1704, 1796
Bse3DI GCAATG 2 cut(s) 142, 3057
Bse8I GATNNNNATC 2 cut(s) 1086, 2856
BseBI CCWGG 1 cut(s) 1931
BseDI CCNNGG 2 cut(s) 1618, 2570
BseGI GGATG 2 cut(s) 2735, 3124
BseJI GATNNNNATC 2 cut(s) 1086, 2856
BseLI CCNNNNNNNGG 7 cut(s) 738, 1570, 2006, 2081, 2117, 2935, 3168
BseMI GCAATG 2 cut(s) 142, 3057
BseMII CTCAG 8 cut(s) 141, 1221, 1344, 1506, 2484, 2613, 2810, 3125
BseNI ACTGG 6 cut(s) 752, 946, 1371, 1504, 1704, 1796
BseRI GAGGAG 5 cut(s) 74, 283, 757, 1004, 3053
BseSI GKGCMC 3 cut(s) 1902, 2322, 3002
BseX3I CGGCCG 2 cut(s) 287, 3078
BseXI GCAGC 8 cut(s) 171, 187, 797, 1037, 1771, 2365, 2488, 2979
BseYI CCCAGC 1 cut(s) 626
BsgI GTGCAG 2 cut(s) 1848, 2173
Bsh1236I CGCG 1 cut(s) 2746
Bsh1285I CGRYCG 4 cut(s) 20, 207, 290, 3081
BshFI GGCC 9 cut(s) 289, 731, 760, 1076, 1433, 1485, 1685, 2736, 3080
BshNI GGYRCC 1 cut(s) 1293
BsiEI CGRYCG 4 cut(s) 20, 207, 290, 3081
BsiHKAI GWGCWC 5 cut(s) 1398, 1902, 2322, 2618, 3002
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 6 cut(s) 168, 761, 1486, 2698, 3081, 3116
BsiWI CGTACG 1 cut(s) 3096
BslFI GGGAC 1 cut(s) 2589
BslI CCNNNNNNNGG 7 cut(s) 738, 1570, 2006, 2081, 2117, 2935, 3168
BsmAI GTCTC 3 cut(s) 1997, 2059, 2437
BsmFI GGGAC 1 cut(s) 2589
BsmI GAATGC 1 cut(s) 1913
BsnI GGCC 9 cut(s) 289, 731, 760, 1076, 1433, 1485, 1685, 2736, 3080
Bso31I GGTCTC 1 cut(s) 2437
BsoBI CYCGRG 1 cut(s) 279
Bsp1286I GDGCHC 6 cut(s) 1208, 1398, 1902, 2322, 2618, 3002
Bsp1407I TGTACA 1 cut(s) 951
Bsp143I GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 3021
BspANI GGCC 9 cut(s) 289, 731, 760, 1076, 1433, 1485, 1685, 2736, 3080
BspCNI CTCAG 8 cut(s) 140, 1220, 1345, 1507, 2483, 2612, 2811, 3126
BspFNI CGCG 1 cut(s) 2746
BspHI TCATGA 3 cut(s) 2095, 2965, 3024
BspLI GGNNCC 9 cut(s) 166, 1295, 1389, 1591, 2109, 2185, 2327, 2577, 2735
BspMAI CTGCAG 1 cut(s) 2997
BspMI ACCTGC 1 cut(s) 2119
BspPI GGATC 2 cut(s) 2670, 3029
BspT107I GGYRCC 1 cut(s) 1293
BspTNI GGTCTC 1 cut(s) 2437
BsrBI CCGCTC 2 cut(s) 2548, 2794
BsrDI GCAATG 2 cut(s) 142, 3057
BsrFI RCCGGY 2 cut(s) 760, 3080
BsrGI TGTACA 1 cut(s) 951
BsrI ACTGG 6 cut(s) 752, 946, 1371, 1504, 1704, 1796
BssAI RCCGGY 2 cut(s) 760, 3080
BssECI CCNNGG 2 cut(s) 1618, 2570
BssMI GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 3021
BssT1I CCWWGG 2 cut(s) 1618, 2570
Bst2UI CCWGG 1 cut(s) 1931
Bst6I CTCTTC 1 cut(s) 95
BstAPI GCANNNNNTGC 3 cut(s) 2231, 2853, 2992
BstAUI TGTACA 1 cut(s) 951
BstBAI YACGTR 1 cut(s) 2989
BstC8I GCNNGC 7 cut(s) 336, 762, 789, 971, 1350, 2245, 2390
BstDEI CTNAG 9 cut(s) 127, 1207, 1353, 1515, 2126, 2470, 2599, 2819, 3134
BstENI CCTNNNNNAGG 2 cut(s) 2004, 2079
BstF5I GGATG 2 cut(s) 2735, 3124
BstFNI CGCG 1 cut(s) 2746
BstHHI GCGC 1 cut(s) 2746
BstKTI GATC 8 cut(s) 1096, 1173, 1872, 2528, 2665, 2818, 2871, 3024
BstMAI GTCTC 3 cut(s) 1997, 2059, 2437
BstMBI GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 3021
BstMCI CGRYCG 4 cut(s) 20, 207, 290, 3081
BstNI CCWGG 1 cut(s) 1931
BstNSI RCATGY 3 cut(s) 1352, 1962, 2199
BstSCI CCNGG 2 cut(s) 1929, 2697
BstSFI CTRYAG 2 cut(s) 2993, 3166
BstSLI GKGCMC 3 cut(s) 1902, 2322, 3002
BstUI CGCG 1 cut(s) 2746
BstV1I GCAGC 8 cut(s) 171, 187, 797, 1037, 1771, 2365, 2488, 2979
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 2815
BstXI CCANNNNNNTGG 1 cut(s) 1304
BstYI RGATCY 1 cut(s) 2815
BstZI CGGCCG 2 cut(s) 287, 3078
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 9 cut(s) 289, 731, 760, 1076, 1433, 1485, 1685, 2736, 3080
BtsCI GGATG 2 cut(s) 2735, 3124
BtsI GCAGTG 5 cut(s) 402, 807, 1002, 2263, 3002
BtsIMutI CAGTG 9 cut(s) 402, 807, 1002, 1466, 1697, 2025, 2263, 2607, 3002
BveI ACCTGC 1 cut(s) 2119
Cac8I GCNNGC 7 cut(s) 336, 762, 789, 971, 1350, 2245, 2390
CaiI CAGNNNCTG 4 cut(s) 1117, 1214, 2342, 2477
CciI TCATGA 3 cut(s) 2095, 2965, 3024
CfoI GCGC 1 cut(s) 2746
Cfr10I RCCGGY 2 cut(s) 760, 3080
Cfr13I GGNCC 7 cut(s) 255, 729, 1387, 1589, 2325, 2576, 2734
CseI GACGC 1 cut(s) 561
Csp6I GTAC 8 cut(s) 121, 952, 1556, 1789, 2169, 2358, 2833, 3097
CspCI CAANNNNNGTGG 2 cut(s) 2014, 2049
CviQI GTAC 8 cut(s) 121, 952, 1556, 1789, 2169, 2358, 2833, 3097
DdeI CTNAG 9 cut(s) 127, 1207, 1353, 1515, 2126, 2470, 2599, 2819, 3134
DpnI GATC 8 cut(s) 1095, 1172, 1871, 2527, 2664, 2817, 2870, 3023
DpnII GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 3021
DraIII CACNNNGTG 1 cut(s) 2043
EaeI YGGCCR 4 cut(s) 287, 758, 1483, 3078
EagI CGGCCG 2 cut(s) 287, 3078
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
EciI GGCGGA 2 cut(s) 80, 121
EclXI CGGCCG 2 cut(s) 287, 3078
Eco130I CCWWGG 2 cut(s) 1618, 2570
Eco24I GRGCYC 1 cut(s) 1208
Eco31I GGTCTC 1 cut(s) 2437
Eco32I GATATC 2 cut(s) 454, 1975
Eco47I GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
Eco52I CGGCCG 2 cut(s) 287, 3078
Eco57I CTGAAG 4 cut(s) 959, 2097, 2398, 2469
Eco72I CACGTG 1 cut(s) 2989
Eco88I CYCGRG 1 cut(s) 279
EcoNI CCTNNNNNAGG 2 cut(s) 2004, 2079
EcoO109I RGGNCCY 3 cut(s) 1387, 2325, 2734
EcoRII CCWGG 1 cut(s) 1929
EcoRV GATATC 2 cut(s) 454, 1975
EcoT14I CCWWGG 2 cut(s) 1618, 2570
EcoT38I GRGCYC 1 cut(s) 1208
ErhI CCWWGG 2 cut(s) 1618, 2570
FaqI GGGAC 1 cut(s) 2589
FauI CCCGC 1 cut(s) 2787
FauNDI CATATG 3 cut(s) 1277, 1569, 1874
FbaI TGATCA 2 cut(s) 1170, 1869
FokI GGATG 2 cut(s) 2742, 3131
FriOI GRGCYC 1 cut(s) 1208
FspBI CTAG 1 cut(s) 3069
GlaI GCGC 1 cut(s) 2745
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 2 cut(s) 77, 777
HaeIII GGCC 9 cut(s) 289, 731, 760, 1076, 1433, 1485, 1685, 2736, 3080
HapII CCGG 6 cut(s) 168, 761, 1486, 2698, 3081, 3116
HgaI GACGC 1 cut(s) 561
HhaI GCGC 1 cut(s) 2746
Hin6I GCGC 1 cut(s) 2744
HinP1I GCGC 1 cut(s) 2744
HincII GTYRAC 1 cut(s) 2035
HindII GTYRAC 1 cut(s) 2035
HindIII AAGCTT 1 cut(s) 2386
HpaII CCGG 6 cut(s) 168, 761, 1486, 2698, 3081, 3116
Hpy166II GTNNAC 8 cut(s) 962, 1235, 1589, 1812, 1900, 2035, 2320, 3000
Hpy8I GTNNAC 8 cut(s) 962, 1235, 1589, 1812, 1900, 2035, 2320, 3000
Hpy99I CGWCG 3 cut(s) 178, 188, 577
HpyCH4IV ACGT 4 cut(s) 580, 2803, 2988, 3095
HpyF3I CTNAG 9 cut(s) 127, 1207, 1353, 1515, 2126, 2470, 2599, 2819, 3134
HpySE526I ACGT 4 cut(s) 580, 2803, 2988, 3095
HspAI GCGC 1 cut(s) 2744
KroI GCCGGC 1 cut(s) 760
KroNI GCCGGC 1 cut(s) 762
Ksp22I TGATCA 2 cut(s) 1170, 1869
Kzo9I GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 3021
LmnI GCTCC 5 cut(s) 170, 623, 744, 796, 3163
Lsp1109I GCAGC 8 cut(s) 171, 187, 797, 1037, 1771, 2365, 2488, 2979
LweI GCATC 6 cut(s) 1599, 2243, 2329, 2498, 2881, 2959
MaeI CTAG 1 cut(s) 3069
MaeII ACGT 4 cut(s) 580, 2803, 2988, 3095
MaeIII GTNAC 7 cut(s) 536, 1358, 1455, 1495, 1519, 2290, 3125
MalI GATC 8 cut(s) 1095, 1172, 1871, 2527, 2664, 2817, 2870, 3023
MbiI CCGCTC 2 cut(s) 2548, 2794
MboI GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 3021
MfeI CAATTG 1 cut(s) 3007
MflI RGATCY 1 cut(s) 2815
MhlI GDGCHC 6 cut(s) 1208, 1398, 1902, 2322, 2618, 3002
MlyI GAGTC 6 cut(s) 197, 227, 1610, 2170, 2483, 2591
MmeI TCCRAC 1 cut(s) 2098
MroNI GCCGGC 1 cut(s) 760
MroXI GAANNNNTTC 1 cut(s) 2408
MslI CAYNNNNRTG 4 cut(s) 1302, 1508, 2220, 3029
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 6 cut(s) 168, 761, 1486, 2698, 3081, 3116
MspR9I CCNGG 2 cut(s) 1931, 2699
MunI CAATTG 1 cut(s) 3007
Mva1269I GAATGC 1 cut(s) 1913
MvaI CCWGG 1 cut(s) 1931
MvnI CGCG 1 cut(s) 2746
NaeI GCCGGC 1 cut(s) 762
NciI CCSGG 1 cut(s) 2699
NdeI CATATG 3 cut(s) 1277, 1569, 1874
NdeII GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 3021
NgoMIV GCCGGC 1 cut(s) 760
NlaIV GGNNCC 9 cut(s) 166, 1295, 1389, 1591, 2109, 2185, 2327, 2577, 2735
NmuCI GTSAC 3 cut(s) 1358, 1495, 3125
NspI RCATGY 3 cut(s) 1352, 1962, 2199
PacI TTAATTAA 1 cut(s) 2925
PaeI GCATGC 1 cut(s) 1352
PaeR7I CTCGAG 1 cut(s) 279
PagI TCATGA 3 cut(s) 2095, 2965, 3024
PciI ACATGT 1 cut(s) 2195
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 572
PctI GAATGC 1 cut(s) 1913
PdiI GCCGGC 1 cut(s) 762
PdmI GAANNNNTTC 1 cut(s) 2408
Pfl23II CGTACG 1 cut(s) 3096
PflMI CCANNNNNTGG 1 cut(s) 2935
PfoI TCCNGGA 1 cut(s) 1929
PleI GAGTC 6 cut(s) 196, 226, 1609, 2170, 2482, 2591
PmaCI CACGTG 1 cut(s) 2989
PmlI CACGTG 1 cut(s) 2989
PpsI GAGTC 6 cut(s) 196, 226, 1609, 2170, 2482, 2591
Ppu21I YACGTR 1 cut(s) 2989
PpuMI RGGWCCY 2 cut(s) 1387, 2325
PscI ACATGT 1 cut(s) 2195
Psp5II RGGWCCY 2 cut(s) 1387, 2325
Psp6I CCWGG 1 cut(s) 1929
PspCI CACGTG 1 cut(s) 2989
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 1 cut(s) 1929
PspLI CGTACG 1 cut(s) 3096
PspN4I GGNNCC 9 cut(s) 166, 1295, 1389, 1591, 2109, 2185, 2327, 2577, 2735
PspPI GGNCC 7 cut(s) 255, 729, 1387, 1589, 2325, 2576, 2734
PspPPI RGGWCCY 2 cut(s) 1387, 2325
PspXI VCTCGAGB 1 cut(s) 279
PsrI GAACNNNNNNTAC 2 cut(s) 1719, 1751
PstI CTGCAG 1 cut(s) 2997
PstNI CAGNNNCTG 4 cut(s) 1117, 1214, 2342, 2477
PsuI RGATCY 1 cut(s) 2815
RsaI GTAC 8 cut(s) 122, 953, 1557, 1790, 2170, 2359, 2834, 3098
RsaNI GTAC 8 cut(s) 121, 952, 1556, 1789, 2169, 2358, 2833, 3097
RseI CAYNNNNRTG 4 cut(s) 1302, 1508, 2220, 3029
Sau3AI GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 3021
Sau96I GGNCC 7 cut(s) 255, 729, 1387, 1589, 2325, 2576, 2734
SchI GAGTC 6 cut(s) 197, 227, 1610, 2170, 2483, 2591
ScrFI CCNGG 2 cut(s) 1931, 2699
SduI GDGCHC 6 cut(s) 1208, 1398, 1902, 2322, 2618, 3002
SfaNI GCATC 6 cut(s) 1599, 2243, 2329, 2498, 2881, 2959
SfcI CTRYAG 2 cut(s) 2993, 3166
Sfr274I CTCGAG 1 cut(s) 279
SinI GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
SlaI CTCGAG 1 cut(s) 279
SmiMI CAYNNNNRTG 4 cut(s) 1302, 1508, 2220, 3029
SmlI CTYRAG 3 cut(s) 279, 923, 2611
SmoI CTYRAG 3 cut(s) 279, 923, 2611
SpeI ACTAGT 1 cut(s) 3068
SphI GCATGC 1 cut(s) 1352
SspI AATATT 3 cut(s) 488, 1268, 2755
SspMI CTAG 1 cut(s) 3069
StyD4I CCNGG 2 cut(s) 1929, 2697
StyI CCWWGG 2 cut(s) 1618, 2570
TaiI ACGT 4 cut(s) 583, 2806, 2991, 3098
TatI WGTACW 1 cut(s) 951
TauI GCSGC 7 cut(s) 16, 165, 238, 292, 645, 700, 2551
TscAI CASTG 9 cut(s) 402, 807, 1002, 1466, 1704, 2032, 2270, 2607, 3002
TseFI GTSAC 3 cut(s) 1358, 1495, 3125
TseI GCWGC 8 cut(s) 159, 200, 810, 1025, 1759, 2378, 2501, 2992
Tsp45I GTSAC 3 cut(s) 1358, 1495, 3125
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 9 cut(s) 402, 807, 1002, 1466, 1704, 2032, 2270, 2607, 3002
Van91I CCANNNNNTGG 1 cut(s) 2935
VneI GTGCAC 3 cut(s) 1898, 2318, 2998
VpaK11BI GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
XagI CCTNNNNNAGG 2 cut(s) 2004, 2079
XapI RAATTY 9 cut(s) 37, 223, 562, 589, 1365, 2530, 2643, 2976, 3173
XceI RCATGY 3 cut(s) 1352, 1962, 2199
XcmI CCANNNNNNNNNTGG 3 cut(s) 1304, 1910, 2276
XhoI CTCGAG 1 cut(s) 279
XmnI GAANNNNTTC 1 cut(s) 2408
XspI CTAG 1 cut(s) 3069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.