Rw1G025230
ERF Family

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX MLL subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003180
Physical Location & Seq
Reverse (-)
51808487 .. 51816541
8055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025230.1

Sequence Viewer

Length: 3123 bp
ATGATAATTAAGCGGCGGTCGAGATTTGAAATGCCGAATTTGAAACGGTGTAAGCTGGAGGAGGCGGAAGCTAAAGCCTATGCGTATTCGGCGAATCCGAAGAGGCGGAAAGCGAATGGGTACTGCTCAGTTGGCAATGGCGAGGGGGAGGATTTGAGCAGCGGCTCCGGTTCGTCGGGATACGACGAGTCGTTTTCGGCTGCGGTCGAGGTGGTGGAGTCGAATTTGAGTAAGCGGCGAGAAATGAATCGGAGGTCCGAGAAGTTGAGGAGGTCTGCTCGAGGACGGCCGCAAGTGCCGTCTTCGAGGTTTAACGGTTCAGGTGAGAAAAAGCAAGCGAAATATGTAGATACATATACGGTTTTTGAAGTTGAAGAAGAATGTGATGTTTTAGCAGTGGAGGACAGAGAAGGGATTGAGAAATTGGGTTTTGTTAAAAAGCGAAAGGAGGATATCAATGGGTTTAAGAAGTTGAAGAAGTTCCAATATTGTGGGAAGGTAGAGCAAGAAGAAGTGGATTATTATGATGATATTGGTTACAGACAGTATATGGGTTTGAAAAATTCGATAGCGTCGAAACGTGGTGGAAATTCTTTGCCAGAGGAAAGAAGTGAGGAATATGGAGCTGGGTTTAGTTTTGGCGGCAAGGAGAAAGTTCGCAAACCCGATTGTGCAAAGAAGAAAGAAGTGTATAAGCCGCAGGACTTTGCTTTGGGTGATATTGTTTGGGCTAAGTGTGGGAGGAGCTACCCAGTTTGGCCGGCGGTGGTTATCGACCCCATATTGCAAGCTCCAGAAGCAGTGTTGAGCTGCTGTATTCCTAATGCAATCTGTGTCATGTTCTTTGGGTTCTCAAAGAATGGAACACAAAGGGATTATGGGTGGGTGAAGCAGGGAATGATATATCCGTTCAAGCAGTTCCTTGAGAGATTTCGAGGACAGACCCAGTTGTTCAGGAGTAAACCAAGTGAGCTTCAGATGGCAATAGAGGAGGCAGTGTTAGCAGAAAATGGTTTTATTTACGCAGCTTGTAAAACTACTGATTCAGAGGCAAATGCCCCTGAAATCCAAGAGGCCACAGATTTTTATCAAGATCAGGCATATTACAGTCAGAATCTGAATCAGGAAGCATATCAGAAGGACACAAGACCTTGTGATTGCTGTGGTTTGATCACTCCTTCTAAAGCTATGAAAAAAGTGAAGGGCTCAGGTTCTGAAACCCAATTTTTATGTAAACATTGTGCAAAGTTGAAAAAATCAAGACAATATTGTGGCATATGTAAGAAGATATGGCACCATTCAGATGGTGGAGATTGGGTATGCTGTGATGGCTGTAATGTTTGGGTGCATGCTGAGTGTGACAAAATTTCCAGTAAAGTTTTCAAGGACCCAGAGCACATTGATTACTATTGTCCTGATTGCAAAAATAAGGCCAAAGGTGAAATGTTAGATGAGTTACAGTGTCGGCAAAAACACAGTTCTGGCCGGAAATCTGTGACTGGCGTAACTGTGTCTGAGGTAACTGTGGTTTGTAATGGCATGGAAGGAACTTATGTACCAAACCTTCATATGGTTGCTTGTAAGTGTGGACCCTGTGATGAGTCAAAGATGCAGAGCCTTGGTGAATGGGAAAGGCACACAGGTTGTAGAGCAAAAAAATGGAAGCATAGTGTGAAAGTGAAGGCCACAATGCTAACACTGGAACAATGGATTGCAGAGTATAATGAACATGGCTTACAGCCTCTCAAATTAGATAAGCAGCGACTACTTGATTCCTTACAAGAAAAGTACGAGCCAGTTAATGCAAAGTGGACAAGTGAAAGATGTGCAGTTTGTAGATGGGTTGAAGATTGGGAAGATAACAAAATGATCATATGCAACAGGTGTCAAATAGCTGTGCACCAAGAATGCTATGGTGCAAAAGATGTCCAGGATTTTACTTCATGGGTTTGTAGAGCATGTGAAACACCTGATATCATTAAAGAATGTTGTCTCTGTCCTGTAAAAGGAGGTGCTTTGAAGCCCACTGATGTTGACACCTTGTGGGTTCATGTCTCTTGTGCTTGGTTTCGTCCTGAAGTAGGTTTCTTGAATCATGAGAAGATGGAACCTGCTGTTGGAATCCTTAGAATCCCACCCACTACTTTTATGAAGCGTTGCGTAATTTGTACGCAGACTCATGGTTCCTGCACCACATGTTGCAAATGTGCCACTCATTTTCATGCAACCTGTGCATCAAGAGCAGGCTACAACATGGAATTGCACTGCCAAGAGAAGAATGGGAAACAAGTAACAACGAAGATGATATATTGTGCTGTGCACAGGACCCCTAATCCAGATGCTGTTGTTGTTGTTCGTACTCCTTCGGGGGTTTTTGCTGCCAGAAGCTTGCTTCAAAACGAGAAAGGGTTCTTCAGGGGTTCGAGGGTGGTCTCATCAAAGAGGACTGAAGTTCCAGAACCTTCAACCTCAGAGTCTGATGAGTTAGAACCACTCTCTGCTGCAAGATGCCGTGCTATTAAAAGATCAAATTTTAAGGGAGGTGAGCGGCTACCAATTTTTCATCGACCAAGGGGACCAACACATCATTCATTAGACTCAGTGAATAGCTTGAGCACATTTATAGAAGTAGAAGATTCTAAAATTTTTTCTTCCTTCAAGGATCGGCTTTACCACTTGCAGAAAACTGAAAATCACCGGGTTTGTTTTGGTAAATCTGGAATACATGGATGGGGCCTATTTGCGCGTAGGAATATTCAAGAAGGAGAAATGGTTGTTGAGTATCGTGGTGAGCGGGTAAGACGTAGTGTTGCAGATCTGAGGGAAGCACGGTACAGGCTACAAGGCAAAGATTGCTATCTTTTCAAGATCAGCGAGGAAGTAGTAATTGATGCTACATATAGAGGAAATATAGCACGGTTAATTAACCATTCGTGCACACCCAATTGCTATTCAAGGATCATGAGTGTGGGTGAGGAGAACCGAATAGTTCTCATTGCCAAGACTAGTGTTTCGGCTGGTGAAGAACTAACGTACGATTACTTGTTTGAGCCGGATGAATGTGACGAGCTGAGAGTTCCCTGTCTATGTAAAGCTCCCAACTGTAGGAAATTCTTGTGTTAG

Protein Analysis

1040

Amino Acids

118.09

Weight (kDa)

8.58

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 239 - 334 1.3e-14 PWWP domain
PHD PF00628 424 - 474 6.7e-07 PHD-finger
PHD PF00628 609 - 654 9.5e-06 PHD-finger
PHD_2 PF13831 621 - 655 1.4e-12 PHD-finger
zf-HC5HC2H_2 PF13832 662 - 774 5.4e-26 PHD-zinc-finger like domain
zf-HC5HC2H PF13771 684 - 775 1.2e-10 PHD-like zinc-binding domain
SET PF00856 910 - 1014 1e-26 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2119
AccB1I GGYRCC 1 cut(s) 1293
AccBSI CCGCTC 2 cut(s) 2548, 2794
AccII CGCG 1 cut(s) 2746
AclWI GGATC 2 cut(s) 2670, 2966
AcoI YGGCCR 3 cut(s) 287, 758, 1483
AcsI RAATTY 8 cut(s) 37, 223, 562, 589, 1365, 2530, 2643, 3110
AcuI CTGAAG 4 cut(s) 959, 2097, 2398, 2469
AdeI CACNNNGTG 1 cut(s) 2043
AfaI GTAC 7 cut(s) 122, 1557, 1790, 2170, 2359, 2834, 3035
AfiI CCNNNNNNNGG 5 cut(s) 1570, 2006, 2081, 2117, 3105
AflIII ACRYGT 1 cut(s) 2195
AhlI ACTAGT 1 cut(s) 3005
AjnI CCWGG 1 cut(s) 1929
AjuI GAANNNNNNNTTGG 4 cut(s) 2100, 2132, 2572, 2604
Alw21I GWGCWC 5 cut(s) 1398, 1902, 2322, 2618, 2939
Alw26I GTCTC 3 cut(s) 1997, 2059, 2437
Alw44I GTGCAC 3 cut(s) 1898, 2318, 2935
AlwI GGATC 2 cut(s) 2670, 2966
AlwNI CAGNNNCTG 4 cut(s) 1117, 1214, 2342, 2477
Ama87I CYCGRG 1 cut(s) 279
AoxI GGCC 7 cut(s) 287, 758, 1074, 1431, 1483, 1683, 2734
ApaLI GTGCAC 3 cut(s) 1898, 2318, 2935
ApeKI GCWGC 7 cut(s) 159, 200, 810, 1025, 1759, 2378, 2501
ApoI RAATTY 8 cut(s) 37, 223, 562, 589, 1365, 2530, 2643, 3110
ArsI GACNNNNNNTTYG 2 cut(s) 2995, 3027
Asp700I GAANNNNTTC 2 cut(s) 479, 2408
AspLEI GCGC 1 cut(s) 2746
AspS9I GGNCC 6 cut(s) 255, 1387, 1589, 2325, 2576, 2734
AsuC2I CCSGG 1 cut(s) 2699
AvaI CYCGRG 1 cut(s) 279
AvaII GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
BaeGI GKGCMC 3 cut(s) 1902, 2322, 2939
BaeI ACNNNNGTAYC 2 cut(s) 1539, 1572
BanI GGYRCC 1 cut(s) 1293
BanII GRGCYC 1 cut(s) 1208
BarI GAAGNNNNNNTAC 2 cut(s) 1548, 1580
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 5 cut(s) 1398, 1902, 2322, 2618, 2939
BbvI GCAGC 7 cut(s) 171, 187, 797, 1037, 1771, 2365, 2488
BccI CCATC 6 cut(s) 973, 1298, 1322, 1833, 2098, 2724
BceAI ACGGC 3 cut(s) 283, 302, 2496
BciT130I CCWGG 1 cut(s) 1931
BciVI GTATCC 1 cut(s) 173
BclI TGATCA 2 cut(s) 1170, 1869
BcnI CCSGG 1 cut(s) 2699
BcoDI GTCTC 3 cut(s) 1997, 2059, 2437
BcuI ACTAGT 1 cut(s) 3005
BfaI CTAG 1 cut(s) 3006
BfmI CTRYAG 1 cut(s) 3103
BfuAI ACCTGC 1 cut(s) 2119
BfuI GTATCC 1 cut(s) 173
BglII AGATCT 1 cut(s) 2815
Bme1390I CCNGG 2 cut(s) 1931, 2699
Bme18I GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
BmeT110I CYCGRG 1 cut(s) 279
BmgT120I GGNCC 6 cut(s) 255, 1387, 1589, 2325, 2576, 2734
BmiI GGNNCC 9 cut(s) 166, 1295, 1389, 1591, 2109, 2185, 2327, 2577, 2735
BmrFI CCNGG 2 cut(s) 1931, 2699
BmrI ACTGGG 2 cut(s) 746, 940
BmsI GCATC 5 cut(s) 1599, 2243, 2329, 2498, 2881
BmuI ACTGGG 2 cut(s) 746, 940
BpiI GAAGAC 1 cut(s) 294
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpmI CTGGAG 2 cut(s) 77, 777
Bpu10I CCTNAGC 1 cut(s) 1207
BpuEI CTTGAG 2 cut(s) 944, 2632
BpuMI CCSGG 1 cut(s) 2699
BsaBI GATNNNNATC 2 cut(s) 1086, 2856
BsaI GGTCTC 1 cut(s) 2437
BsaJI CCNNGG 2 cut(s) 1618, 2570
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 6 cut(s) 983, 1013, 1302, 1332, 2757, 2787
Bsc4I CCNNNNNNNGG 5 cut(s) 1570, 2006, 2081, 2117, 3105
Bse118I RCCGGY 1 cut(s) 760
Bse1I ACTGG 6 cut(s) 752, 946, 1371, 1504, 1704, 1796
Bse3DI GCAATG 2 cut(s) 142, 2994
Bse8I GATNNNNATC 2 cut(s) 1086, 2856
BseBI CCWGG 1 cut(s) 1931
BseDI CCNNGG 2 cut(s) 1618, 2570
BseGI GGATG 2 cut(s) 2735, 3061
BseJI GATNNNNATC 2 cut(s) 1086, 2856
BseLI CCNNNNNNNGG 5 cut(s) 1570, 2006, 2081, 2117, 3105
BseMI GCAATG 2 cut(s) 142, 2994
BseMII CTCAG 8 cut(s) 141, 1221, 1344, 1506, 2484, 2613, 2810, 3062
BseNI ACTGG 6 cut(s) 752, 946, 1371, 1504, 1704, 1796
BseRI GAGGAG 5 cut(s) 74, 283, 757, 1004, 2990
BseSI GKGCMC 3 cut(s) 1902, 2322, 2939
BseX3I CGGCCG 1 cut(s) 287
BseXI GCAGC 7 cut(s) 171, 187, 797, 1037, 1771, 2365, 2488
BseYI CCCAGC 1 cut(s) 626
BsgI GTGCAG 2 cut(s) 1848, 2173
Bsh1236I CGCG 1 cut(s) 2746
Bsh1285I CGRYCG 3 cut(s) 20, 207, 290
BshFI GGCC 7 cut(s) 289, 760, 1076, 1433, 1485, 1685, 2736
BshNI GGYRCC 1 cut(s) 1293
BsiEI CGRYCG 3 cut(s) 20, 207, 290
BsiHKAI GWGCWC 5 cut(s) 1398, 1902, 2322, 2618, 2939
BsiHKCI CYCGRG 1 cut(s) 279
BsiSI CCGG 5 cut(s) 168, 761, 1486, 2698, 3053
BsiWI CGTACG 1 cut(s) 3033
BslFI GGGAC 1 cut(s) 2589
BslI CCNNNNNNNGG 5 cut(s) 1570, 2006, 2081, 2117, 3105
BsmAI GTCTC 3 cut(s) 1997, 2059, 2437
BsmFI GGGAC 1 cut(s) 2589
BsmI GAATGC 1 cut(s) 1913
BsnI GGCC 7 cut(s) 289, 760, 1076, 1433, 1485, 1685, 2736
Bso31I GGTCTC 1 cut(s) 2437
BsoBI CYCGRG 1 cut(s) 279
Bsp1286I GDGCHC 6 cut(s) 1208, 1398, 1902, 2322, 2618, 2939
Bsp143I GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 2958
BspANI GGCC 7 cut(s) 289, 760, 1076, 1433, 1485, 1685, 2736
BspCNI CTCAG 8 cut(s) 140, 1220, 1345, 1507, 2483, 2612, 2811, 3063
BspFNI CGCG 1 cut(s) 2746
BspHI TCATGA 2 cut(s) 2095, 2961
BspLI GGNNCC 9 cut(s) 166, 1295, 1389, 1591, 2109, 2185, 2327, 2577, 2735
BspMI ACCTGC 1 cut(s) 2119
BspPI GGATC 2 cut(s) 2670, 2966
BspT107I GGYRCC 1 cut(s) 1293
BspTNI GGTCTC 1 cut(s) 2437
BsrBI CCGCTC 2 cut(s) 2548, 2794
BsrDI GCAATG 2 cut(s) 142, 2994
BsrFI RCCGGY 1 cut(s) 760
BsrI ACTGG 6 cut(s) 752, 946, 1371, 1504, 1704, 1796
BssAI RCCGGY 1 cut(s) 760
BssECI CCNNGG 2 cut(s) 1618, 2570
BssMI GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 2958
BssT1I CCWWGG 2 cut(s) 1618, 2570
Bst2UI CCWGG 1 cut(s) 1931
Bst6I CTCTTC 1 cut(s) 95
BstAPI GCANNNNNTGC 2 cut(s) 2231, 2853
BstC8I GCNNGC 6 cut(s) 336, 762, 789, 1350, 2245, 2390
BstENI CCTNNNNNAGG 2 cut(s) 2004, 2079
BstF5I GGATG 2 cut(s) 2735, 3061
BstFNI CGCG 1 cut(s) 2746
BstHHI GCGC 1 cut(s) 2746
BstKTI GATC 8 cut(s) 1096, 1173, 1872, 2528, 2665, 2818, 2871, 2961
BstMAI GTCTC 3 cut(s) 1997, 2059, 2437
BstMBI GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 2958
BstMCI CGRYCG 3 cut(s) 20, 207, 290
BstMWI GCNNNNNNNGC 9 cut(s) 89, 132, 295, 797, 1001, 1329, 2231, 2240, 2853
BstNI CCWGG 1 cut(s) 1931
BstNSI RCATGY 3 cut(s) 1352, 1962, 2199
BstSCI CCNGG 2 cut(s) 1929, 2697
BstSFI CTRYAG 1 cut(s) 3103
BstSLI GKGCMC 3 cut(s) 1902, 2322, 2939
BstUI CGCG 1 cut(s) 2746
BstV1I GCAGC 7 cut(s) 171, 187, 797, 1037, 1771, 2365, 2488
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 2815
BstXI CCANNNNNNTGG 2 cut(s) 491, 1304
BstYI RGATCY 1 cut(s) 2815
BstZI CGGCCG 1 cut(s) 287
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 7 cut(s) 289, 760, 1076, 1433, 1485, 1685, 2736
BtsCI GGATG 2 cut(s) 2735, 3061
BtsI GCAGTG 4 cut(s) 402, 807, 1002, 2263
BtsIMutI CAGTG 8 cut(s) 402, 807, 1002, 1466, 1697, 2025, 2263, 2607
BveI ACCTGC 1 cut(s) 2119
Cac8I GCNNGC 6 cut(s) 336, 762, 789, 1350, 2245, 2390
CaiI CAGNNNCTG 4 cut(s) 1117, 1214, 2342, 2477
CciI TCATGA 2 cut(s) 2095, 2961
CfoI GCGC 1 cut(s) 2746
Cfr10I RCCGGY 1 cut(s) 760
Cfr13I GGNCC 6 cut(s) 255, 1387, 1589, 2325, 2576, 2734
CseI GACGC 1 cut(s) 561
Csp6I GTAC 7 cut(s) 121, 1556, 1789, 2169, 2358, 2833, 3034
CspCI CAANNNNNGTGG 2 cut(s) 2014, 2049
CviQI GTAC 7 cut(s) 121, 1556, 1789, 2169, 2358, 2833, 3034
DpnI GATC 8 cut(s) 1095, 1172, 1871, 2527, 2664, 2817, 2870, 2960
DpnII GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 2958
DraIII CACNNNGTG 1 cut(s) 2043
EaeI YGGCCR 3 cut(s) 287, 758, 1483
EagI CGGCCG 1 cut(s) 287
Eam1104I CTCTTC 1 cut(s) 95
EarI CTCTTC 1 cut(s) 95
EciI GGCGGA 2 cut(s) 80, 121
EclXI CGGCCG 1 cut(s) 287
Eco130I CCWWGG 2 cut(s) 1618, 2570
Eco24I GRGCYC 1 cut(s) 1208
Eco31I GGTCTC 1 cut(s) 2437
Eco32I GATATC 2 cut(s) 454, 1975
Eco47I GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
Eco52I CGGCCG 1 cut(s) 287
Eco57I CTGAAG 4 cut(s) 959, 2097, 2398, 2469
Eco88I CYCGRG 1 cut(s) 279
EcoNI CCTNNNNNAGG 2 cut(s) 2004, 2079
EcoO109I RGGNCCY 3 cut(s) 1387, 2325, 2734
EcoRII CCWGG 1 cut(s) 1929
EcoRV GATATC 2 cut(s) 454, 1975
EcoT14I CCWWGG 2 cut(s) 1618, 2570
EcoT38I GRGCYC 1 cut(s) 1208
ErhI CCWWGG 2 cut(s) 1618, 2570
FaqI GGGAC 1 cut(s) 2589
FauI CCCGC 1 cut(s) 2787
FauNDI CATATG 3 cut(s) 1277, 1569, 1874
FbaI TGATCA 2 cut(s) 1170, 1869
FokI GGATG 2 cut(s) 2742, 3068
FriOI GRGCYC 1 cut(s) 1208
FspBI CTAG 1 cut(s) 3006
GlaI GCGC 1 cut(s) 2745
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 2 cut(s) 77, 777
HaeIII GGCC 7 cut(s) 289, 760, 1076, 1433, 1485, 1685, 2736
HapII CCGG 5 cut(s) 168, 761, 1486, 2698, 3053
HgaI GACGC 1 cut(s) 561
HhaI GCGC 1 cut(s) 2746
Hin6I GCGC 1 cut(s) 2744
HinP1I GCGC 1 cut(s) 2744
HincII GTYRAC 1 cut(s) 2035
HindII GTYRAC 1 cut(s) 2035
HindIII AAGCTT 1 cut(s) 2386
HpaII CCGG 5 cut(s) 168, 761, 1486, 2698, 3053
Hpy166II GTNNAC 8 cut(s) 962, 1235, 1589, 1812, 1900, 2035, 2320, 2937
Hpy8I GTNNAC 8 cut(s) 962, 1235, 1589, 1812, 1900, 2035, 2320, 2937
Hpy99I CGWCG 3 cut(s) 178, 188, 577
HpyCH4IV ACGT 3 cut(s) 580, 2803, 3032
HpyF10VI GCNNNNNNNGC 9 cut(s) 89, 132, 295, 797, 1001, 1329, 2231, 2240, 2853
HpySE526I ACGT 3 cut(s) 580, 2803, 3032
HspAI GCGC 1 cut(s) 2744
KroI GCCGGC 1 cut(s) 760
KroNI GCCGGC 1 cut(s) 762
Ksp22I TGATCA 2 cut(s) 1170, 1869
Kzo9I GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 2958
LmnI GCTCC 5 cut(s) 170, 623, 744, 796, 3100
Lsp1109I GCAGC 7 cut(s) 171, 187, 797, 1037, 1771, 2365, 2488
LweI GCATC 5 cut(s) 1599, 2243, 2329, 2498, 2881
MaeI CTAG 1 cut(s) 3006
MaeII ACGT 3 cut(s) 580, 2803, 3032
MaeIII GTNAC 8 cut(s) 536, 1358, 1455, 1495, 1504, 1519, 2290, 3062
MalI GATC 8 cut(s) 1095, 1172, 1871, 2527, 2664, 2817, 2870, 2960
MbiI CCGCTC 2 cut(s) 2548, 2794
MboI GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 2958
MfeI CAATTG 1 cut(s) 2944
MflI RGATCY 1 cut(s) 2815
MhlI GDGCHC 6 cut(s) 1208, 1398, 1902, 2322, 2618, 2939
MlyI GAGTC 6 cut(s) 197, 227, 1610, 2170, 2483, 2591
MmeI TCCRAC 1 cut(s) 2098
MroNI GCCGGC 1 cut(s) 760
MroXI GAANNNNTTC 2 cut(s) 479, 2408
MslI CAYNNNNRTG 3 cut(s) 1302, 2220, 2966
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 5 cut(s) 168, 761, 1486, 2698, 3053
MspR9I CCNGG 2 cut(s) 1931, 2699
MunI CAATTG 1 cut(s) 2944
Mva1269I GAATGC 1 cut(s) 1913
MvaI CCWGG 1 cut(s) 1931
MvnI CGCG 1 cut(s) 2746
MwoI GCNNNNNNNGC 9 cut(s) 89, 132, 295, 797, 1001, 1329, 2231, 2240, 2853
NaeI GCCGGC 1 cut(s) 762
NciI CCSGG 1 cut(s) 2699
NdeI CATATG 3 cut(s) 1277, 1569, 1874
NdeII GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 2958
NgoMIV GCCGGC 1 cut(s) 760
NlaIV GGNNCC 9 cut(s) 166, 1295, 1389, 1591, 2109, 2185, 2327, 2577, 2735
NmuCI GTSAC 3 cut(s) 1358, 1495, 3062
NspI RCATGY 3 cut(s) 1352, 1962, 2199
PacI TTAATTAA 1 cut(s) 2925
PaeI GCATGC 1 cut(s) 1352
PaeR7I CTCGAG 1 cut(s) 279
PagI TCATGA 2 cut(s) 2095, 2961
PciI ACATGT 1 cut(s) 2195
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 572
PctI GAATGC 1 cut(s) 1913
PdiI GCCGGC 1 cut(s) 762
PdmI GAANNNNTTC 2 cut(s) 479, 2408
Pfl23II CGTACG 1 cut(s) 3033
PfoI TCCNGGA 1 cut(s) 1929
PleI GAGTC 6 cut(s) 196, 226, 1609, 2170, 2482, 2591
PpsI GAGTC 6 cut(s) 196, 226, 1609, 2170, 2482, 2591
PpuMI RGGWCCY 2 cut(s) 1387, 2325
PscI ACATGT 1 cut(s) 2195
Psp5II RGGWCCY 2 cut(s) 1387, 2325
Psp6I CCWGG 1 cut(s) 1929
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 1 cut(s) 1929
PspLI CGTACG 1 cut(s) 3033
PspN4I GGNNCC 9 cut(s) 166, 1295, 1389, 1591, 2109, 2185, 2327, 2577, 2735
PspPI GGNCC 6 cut(s) 255, 1387, 1589, 2325, 2576, 2734
PspPPI RGGWCCY 2 cut(s) 1387, 2325
PspXI VCTCGAGB 1 cut(s) 279
PsrI GAACNNNNNNTAC 2 cut(s) 1719, 1751
PstNI CAGNNNCTG 4 cut(s) 1117, 1214, 2342, 2477
PsuI RGATCY 1 cut(s) 2815
RsaI GTAC 7 cut(s) 122, 1557, 1790, 2170, 2359, 2834, 3035
RsaNI GTAC 7 cut(s) 121, 1556, 1789, 2169, 2358, 2833, 3034
RseI CAYNNNNRTG 3 cut(s) 1302, 2220, 2966
Sau3AI GATC 8 cut(s) 1093, 1170, 1869, 2525, 2662, 2815, 2868, 2958
Sau96I GGNCC 6 cut(s) 255, 1387, 1589, 2325, 2576, 2734
SchI GAGTC 6 cut(s) 197, 227, 1610, 2170, 2483, 2591
ScrFI CCNGG 2 cut(s) 1931, 2699
SduI GDGCHC 6 cut(s) 1208, 1398, 1902, 2322, 2618, 2939
SfaNI GCATC 5 cut(s) 1599, 2243, 2329, 2498, 2881
SfcI CTRYAG 1 cut(s) 3103
Sfr274I CTCGAG 1 cut(s) 279
SinI GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
SlaI CTCGAG 1 cut(s) 279
SmiMI CAYNNNNRTG 3 cut(s) 1302, 2220, 2966
SmlI CTYRAG 3 cut(s) 279, 923, 2611
SmoI CTYRAG 3 cut(s) 279, 923, 2611
SpeI ACTAGT 1 cut(s) 3005
SphI GCATGC 1 cut(s) 1352
SspI AATATT 3 cut(s) 488, 1268, 2755
SspMI CTAG 1 cut(s) 3006
StyD4I CCNGG 2 cut(s) 1929, 2697
StyI CCWWGG 2 cut(s) 1618, 2570
TaiI ACGT 3 cut(s) 583, 2806, 3035
TauI GCSGC 7 cut(s) 16, 165, 238, 292, 645, 700, 2551
TscAI CASTG 8 cut(s) 402, 807, 1002, 1466, 1704, 2032, 2270, 2607
TseFI GTSAC 3 cut(s) 1358, 1495, 3062
TseI GCWGC 7 cut(s) 159, 200, 810, 1025, 1759, 2378, 2501
Tsp45I GTSAC 3 cut(s) 1358, 1495, 3062
TspGWI ACGGA 1 cut(s) 897
TspRI CASTG 8 cut(s) 402, 807, 1002, 1466, 1704, 2032, 2270, 2607
VneI GTGCAC 3 cut(s) 1898, 2318, 2935
VpaK11BI GGWCC 5 cut(s) 255, 1387, 1589, 2325, 2576
XagI CCTNNNNNAGG 2 cut(s) 2004, 2079
XapI RAATTY 8 cut(s) 37, 223, 562, 589, 1365, 2530, 2643, 3110
XceI RCATGY 3 cut(s) 1352, 1962, 2199
XcmI CCANNNNNNNNNTGG 3 cut(s) 1304, 1910, 2276
XhoI CTCGAG 1 cut(s) 279
XmnI GAANNNNTTC 2 cut(s) 479, 2408
XspI CTAG 1 cut(s) 3006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.