Rroxscaffold_4G00297040
ERF Family

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX MLL subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003180
Physical Location & Seq
Forward (+)
17050847 .. 17063249
12403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00297040.1

Sequence Viewer

Length: 3669 bp
ATGATAATTAAGCGGCCGTCGAGATTTGAAATGCCGAATTTGAAACGGTGTAAGCTGGAGGAGGCGGAAGCTAAAGCCTATGCGTATTCGGCGAATCCGAAGAGGCGGAAAGCGAATGGGTACTGCTCAGTTGGCAATGGCGAGGGGGAGGATTTGAGCAGCGGCTCCGGTTCGTCGGGATACGACGAGTCGTTTTCGGCTGCGGTAGGGGTGGAGTCGAATTTGCAGCGGTTGAGTAAGGTTCGAGAAATGAATCGGAGGTTCGAGAAGTTGAGGAGGTCAGCTCGAGGACGGCCGCAAGTGCCGTCTTCGAGGTTTAACGGTTCAGGTGAGAAAAAGCAAGCGAAATATGTAGATACATATACGATTTTTGAAGAAGAAGAATGTGATGTTTTAGCAGTGGAGGACAGAGAAGGGATTGAGAAATTGGGTTTTGTTAAAAAGCGTAAGGAGGATATCAATGGGTTTAAGAAGCTGAAGAAGTTCCAATATTGTGGGAAGGTAGAGCAAGAAGAAGTGGATTATTATGATGATATTGGTTACAGACAGTATATGGGTTTGAAAAATTCGACAGCGTCGAAACGGGGGAGAAATTCTTTGCCAGAGGAAAGAAGTGAGGAATATGGAGCTGGGCTTAGTTTTGGCGGCAAGGAGAAAGTTAGCAAACCCGATTGTGCAAAGAAGAAAGAAGTGTATAAGCCGCAGGACTTTGCTTTGGGTGATATTGTTTGGGCTAAGTGTGGGAGGAGCTACCCAGTTTGGCCGGCGGTGGTTATCGACCCCATATTGCAAGCTCCAGAAGCAGTGTTGAGCTGCTGTATTCCTAATGCTATCTGTGTCATGTTCTTTGGGTTCTCAAAGAATGGAACACAAAGGGATTATGGGTGGGTGAAGCAGGGAATGATATATCCGTTTAAGCAGTTCCTTGAGAGATTTCGAGGACAGACCCAGTTGTACAGGAGTAAACCTAGCGAGCTTCAGATGGCAATAGAGGAGGCGGTGTTAGCAGAAAATGGCTTTATTTACGCAGCTTGTAAAACTAATGATTCAGAGGCAAATGCCCCTGAAATCCAAGAGGCCACAGATTTTTATCAAGATCAGGCATATTACAGTCAGAATCTGAATCAGGCAAGTTCTTTTCTGGGAATATTTTCTTGTCTCAACATTACAAAGTCTGGTTGTCTCTGGAATGAGAGTTCATCCAATGAATTTATTGTGCAGGAAGCATATCAGAAGGACACAAGACCTTGTGATTGCTGTGGTTTGATCACTCCATCTAAAGCTATGAAAAAAGTGAAGGGCTCAGGTTCTGAAACCCAAGTTTTATGTAAACATTGTGCAAAGTTGAAAAAATCAAGACAATATTGTGGCATATGTAAGAAGATATGGCACCATTCAGATGGTGGAGATTGGGTATGCTGTGATGGCTGTAATGTTTGGGTGCATGCTGAGTGTGACAAAATTTCCAGTAAAGTTTTCAAGGACCCAGAGCACATTGATTACTATTGTCCTGATTGCAAAAATAAGGCCAAAGGTGAAATTTCTAGCCGGAAATCTGTGACTGGCGTAACTGTGTCTGAGGTAACTGTGGTTTGTAATGGCATGGAAGGAACTTATGTACCAAACCTTCATATGGTTGCTTGTAAGTGTGGACCCTGTGATAAGTCAAAGATGCAGAGCCTTGGTGAATGGGAAAGGCACACAGGTTGTAGAGCAAAAAAATGGAAGCATAGTGTGAAAGTGAAGGCCACAATGCTAACACTGGAACAATGGATTGCAGAGTATAATGAACATGGCTTACAGCCTCTCAAATTAGATAAGCAGCGACTACTTGATTCCTTACAAGTGTCGCAGAGGCGGCTGAGCCTTTTGCTTAAGTGTCAAATAGCTGTGCACCAAGAATGCTATGGTGCAAAAGATGTCCAGGATTTTACTTCATGGGTTTGTAGAGCATGTGAAACACCTGATATCATTAAAGAATGTTGTCTCTGTCCTGTAAAAGGAGGTGCTTTGAAGCCCACTGATGTTGACACCTTGTGGGTTCATGTCTCTTGTGCTTGGTTTCGTCCCGAAGTAGGTTTCTTGAATCATGAGAAGATGGAACCTGCTGTTGGAATCCTTAGAATCCCACCCACTACTTTTATGAAGTTGCACTGCCAAGAGAAGAATGGGAAACAAGTAACAACGAAGATGATATATTGTGCTGTGCACAGATGCTGTTGTCGTTGTTCGTACTCCTTCGGGGGTTTTGCTGCCAGAAGCTTGCTTCAAAACGAGAAAGGGTTCTTCAGGGGTTCGAGGGTGGTCTCATCAAAGAGGACTGAAGTTCCAGAACCTTCAACCTCAGAGTCTGATGAGTTAGAACCACTCTATGCTGCAAGATGCCGTGCTATTAAAAGATCAAATTTTAAGAAAACTGAAAATCACCGGGTTTGTTTTGGTAAATCTGGAATACATGGATGGGGTCTATTTGCACGTAGGAATATTCAAGAAGGAGAAATGGTTGTTGAGTATCGTGGTGAGCGGGTAAGACGTAGTGTTGCAGATCTGAGGGAAGCACGGTACAGGCTACAAGGCAAAGATTGCTATCTTTTCAAGATCAGTGAGGAAGTAGTAATTGATGCTACATATAGAGGAAATATAGCACGGTTAATTAACCATTCGTGCACACCCAATTGCTATTCAAGGATCATGAGTGTGGGTGAGGAGAACCGGATAGTTCTCATTGCCAAGACTAGTGTTTCGGCCGGTGAAGAACTAACGTACGATTACTTGTTTGAGCCGGATGAATGTGATGAGCTGAGAGTTCCCTGTCTATGTAAAGCTCCCAACTGTAGGAAATTCTTTTACGAGCAGGACGTTTGGACGGAGATCACAAAGTTTCTGGACGGAAAGTCACTGGTGATGCTCGCTGCGACAAGTCGTTGGTTCCACGCGCTGGTCATGGACGGGAGCATATGGAGGTACTTGTGCTTGCGTGATCTTCAGGTCCTTGCTCCTCGCCACCCGGCGGCGTTTGATTGGAAGAAGCTCTACATTTCGGCTTTCAATGGGAGTCACTCTTACATGTTCCGCCAGCCGGAGAAGCATATCGACTGGATGCGTATCGGTGCATTTTTCCTGGAATCTTCTTCAGTACTCGTAACAGAAAGGCTGAGCACCCCACCGAAATTCCCCAAGGAAGATAGCATTCAGAAAATGTTGGAGTCGAGCGGCAGTTGTGTGTTGAACAATGTGAAGCCTGGGATATGGATTGCAGATCTCCAGCTTGTGCGATGCCCTGTCTGCGAGCTTAATACTTGTGATGGAACAATGCAAACATTGGATGCAAGGCATATTGAACTGTTCTTGAGTGAGGGATACCAGGATGGTAGCTGGGAATATGAACTTATTGGAATTCAGGATATCAAAAAGCATGTTGAGGGTGCTTCTGGAGCTATTTTCGATGTCAAACACCTCAAAGACTCTTCAACTTCAGCAGTCTTCAACCTCAAGTCATGGGTCGGGAAACCGAATGACTGGCAACCGAAGGCTGTAATCACAATGCATGCAGTTGCTGTCAACACAAATCTGCAGAGAAATGAAGGCCTGCAAGTCAAATACCATGTGATGAGAGCTGGAGCTGGAGGAGAAGTTGTATCTATTCGAATCTCGCAGCAGCTTCTGTAG

Protein Analysis

1222

Amino Acids

138.49

Weight (kDa)

8.65

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 240 - 335 9e-15 PWWP domain
PHD PF00628 456 - 506 8.1e-07 PHD-finger
PHD_2 PF13831 626 - 653 1.7e-08 PHD-finger
zf-HC5HC2H_2 PF13832 660 - 741 7.4e-13 PHD-zinc-finger like domain
SET PF00856 820 - 924 1.3e-26 SET domain
F-box-like PF12937 953 - 993 7e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2113
AccB1I GGYRCC 1 cut(s) 1389
AccB7I CCANNNNNTGG 1 cut(s) 2938
AccBSI CCGCTC 2 cut(s) 2524, 3213
AccII CGCG 1 cut(s) 2936
AclWI GGATC 1 cut(s) 2696
AcoI YGGCCR 4 cut(s) 14, 293, 761, 2745
AcuI CTGAAG 7 cut(s) 497, 962, 2272, 2343, 2969, 3117, 3459
AdeI CACNNNGTG 1 cut(s) 2037
AfaI GTAC 8 cut(s) 122, 956, 1620, 2234, 2564, 2765, 2966, 3138
AfiI CCNNNNNNNGG 8 cut(s) 1633, 2000, 2075, 2111, 2835, 2938, 3010, 3079
AflII CTTAAG 1 cut(s) 1874
AflIII ACRYGT 1 cut(s) 3066
AhdI GACNNNNNGTC 1 cut(s) 2893
AhlI ACTAGT 1 cut(s) 2735
AjnI CCWGG 4 cut(s) 1923, 3120, 3241, 3363
AjuI GAANNNNNNNTTGG 2 cut(s) 2094, 2126
AloI GAACNNNNNNTCC 2 cut(s) 1180, 1212
Alw21I GWGCWC 5 cut(s) 1494, 1896, 2211, 2669, 3161
Alw26I GTCTC 5 cut(s) 1163, 1187, 1991, 2053, 2311
Alw44I GTGCAC 3 cut(s) 1892, 2207, 2665
AlwI GGATC 1 cut(s) 2696
AlwNI CAGNNNCTG 6 cut(s) 1120, 1310, 2217, 2351, 3557, 3664
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 8 cut(s) 14, 293, 761, 1077, 1527, 1746, 2745, 3586
ApaLI GTGCAC 3 cut(s) 1892, 2207, 2665
ArsI GACNNNNNNTTYG 2 cut(s) 2725, 2757
Asp700I GAANNNNTTC 3 cut(s) 482, 1150, 2282
AspLEI GCGC 1 cut(s) 2938
AspS9I GGNCC 3 cut(s) 1483, 1652, 2989
AsuC2I CCSGG 2 cut(s) 2429, 3008
AsuII TTCGAA 1 cut(s) 3646
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 3 cut(s) 1483, 1652, 2989
BaeGI GKGCMC 3 cut(s) 1896, 2211, 2669
BaeI ACNNNNGTAYC 2 cut(s) 1602, 1635
BanI GGYRCC 1 cut(s) 1389
BanII GRGCYC 1 cut(s) 1304
BarI GAAGNNNNNNTAC 4 cut(s) 1611, 1643, 3017, 3049
BbsI GAAGAC 2 cut(s) 300, 3475
Bbv12I GWGCWC 5 cut(s) 1494, 1896, 2211, 2669, 3161
BbvI GCAGC 9 cut(s) 171, 187, 238, 800, 1040, 1834, 2239, 2362, 2899
BccI CCATC 8 cut(s) 976, 1282, 1394, 1418, 2092, 2454, 3299, 3362
BceAI ACGGC 3 cut(s) 289, 308, 2370
BciT130I CCWGG 4 cut(s) 1925, 3122, 3243, 3365
BciVI GTATCC 2 cut(s) 173, 3353
BclI TGATCA 1 cut(s) 1266
BcnI CCSGG 2 cut(s) 2429, 3008
BcoDI GTCTC 5 cut(s) 1163, 1187, 1991, 2053, 2311
BcuI ACTAGT 1 cut(s) 2735
BfaI CTAG 3 cut(s) 969, 1545, 2736
BfmI CTRYAG 3 cut(s) 2833, 3572, 3665
BfrI CTTAAG 1 cut(s) 1874
BfuAI ACCTGC 1 cut(s) 2113
BfuI GTATCC 2 cut(s) 173, 3353
BglII AGATCT 2 cut(s) 2545, 3259
BlpI GCTNAGC 2 cut(s) 1862, 3155
BmcAI AGTACT 1 cut(s) 3138
Bme1390I CCNGG 6 cut(s) 1925, 2429, 3008, 3122, 3243, 3365
Bme18I GGWCC 3 cut(s) 1483, 1652, 2989
BmeRI GACNNNNNGTC 1 cut(s) 2893
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 1483, 1652, 2989
BmiI GGNNCC 6 cut(s) 166, 1391, 1485, 1654, 2103, 2930
BmrFI CCNGG 6 cut(s) 1925, 2429, 3008, 3122, 3243, 3365
BmrI ACTGGG 2 cut(s) 749, 943
BmsI GCATC 8 cut(s) 1662, 2204, 2372, 2611, 2895, 3090, 3266, 3316
BmuI ACTGGG 2 cut(s) 749, 943
BpiI GAAGAC 2 cut(s) 300, 3475
BplI GAGNNNNNCTC 2 cut(s) 268, 300
BpmI CTGGAG 6 cut(s) 77, 780, 3248, 3453, 3639, 3645
Bpu10I CCTNAGC 1 cut(s) 1303
Bpu1102I GCTNAGC 2 cut(s) 1862, 3155
Bpu14I TTCGAA 1 cut(s) 3646
BpuEI CTTGAG 3 cut(s) 947, 3370, 3476
BpuMI CCSGG 2 cut(s) 2429, 3008
BsaAI YACGTR 1 cut(s) 2477
BsaBI GATNNNNATC 3 cut(s) 1089, 2586, 3104
BsaI GGTCTC 1 cut(s) 2311
BsaJI CCNNGG 3 cut(s) 1681, 3177, 3242
BsaWI WCCGGW 2 cut(s) 167, 2712
Bsc4I CCNNNNNNNGG 8 cut(s) 1633, 2000, 2075, 2111, 2835, 2938, 3010, 3079
Bse118I RCCGGY 2 cut(s) 763, 2747
Bse1I ACTGG 8 cut(s) 755, 949, 1467, 1567, 1767, 2904, 3101, 3524
Bse3DI GCAATG 2 cut(s) 142, 2724
Bse8I GATNNNNATC 3 cut(s) 1089, 2586, 3104
BseBI CCWGG 4 cut(s) 1925, 3122, 3243, 3365
BseDI CCNNGG 3 cut(s) 1681, 3177, 3242
BseGI GGATG 6 cut(s) 1199, 2465, 2791, 3105, 3331, 3373
BseJI GATNNNNATC 3 cut(s) 1089, 2586, 3104
BseLI CCNNNNNNNGG 8 cut(s) 1633, 2000, 2075, 2111, 2835, 2938, 3010, 3079
BseMI GCAATG 2 cut(s) 142, 2724
BseMII CTCAG 9 cut(s) 141, 1317, 1440, 1569, 1853, 2358, 2540, 2792, 3146
BseNI ACTGG 8 cut(s) 755, 949, 1467, 1567, 1767, 2904, 3101, 3524
BseRI GAGGAG 7 cut(s) 74, 289, 760, 1007, 2720, 2988, 3642
BseSI GKGCMC 3 cut(s) 1896, 2211, 2669
BseX3I CGGCCG 3 cut(s) 14, 293, 2745
BseXI GCAGC 9 cut(s) 171, 187, 238, 800, 1040, 1834, 2239, 2362, 2899
BseYI CCCAGC 2 cut(s) 629, 3375
BsgI GTGCAG 1 cut(s) 1238
Bsh1236I CGCG 1 cut(s) 2936
Bsh1285I CGRYCG 3 cut(s) 17, 296, 2748
BshFI GGCC 8 cut(s) 16, 295, 763, 1079, 1529, 1748, 2747, 3588
BshNI GGYRCC 1 cut(s) 1389
BsiEI CGRYCG 3 cut(s) 17, 296, 2748
BsiHKAI GWGCWC 5 cut(s) 1494, 1896, 2211, 2669, 3161
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 9 cut(s) 168, 764, 1549, 2428, 2713, 2748, 2783, 3008, 3080
BsiWI CGTACG 1 cut(s) 2763
BslFI GGGAC 1 cut(s) 2052
BslI CCNNNNNNNGG 8 cut(s) 1633, 2000, 2075, 2111, 2835, 2938, 3010, 3079
BsmAI GTCTC 5 cut(s) 1163, 1187, 1991, 2053, 2311
BsmFI GGGAC 1 cut(s) 2052
BsmI GAATGC 2 cut(s) 1907, 3189
BsnI GGCC 8 cut(s) 16, 295, 763, 1079, 1529, 1748, 2747, 3588
Bso31I GGTCTC 1 cut(s) 2311
BsoBI CYCGRG 1 cut(s) 285
Bsp119I TTCGAA 1 cut(s) 3646
Bsp1286I GDGCHC 6 cut(s) 1304, 1494, 1896, 2211, 2669, 3161
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 9 cut(s) 1096, 1266, 2399, 2545, 2598, 2688, 2871, 2980, 3259
Bsp1720I GCTNAGC 2 cut(s) 1862, 3155
BspANI GGCC 8 cut(s) 16, 295, 763, 1079, 1529, 1748, 2747, 3588
BspCNI CTCAG 9 cut(s) 140, 1316, 1441, 1570, 1854, 2357, 2541, 2793, 3147
BspFNI CGCG 1 cut(s) 2936
BspHI TCATGA 2 cut(s) 2089, 2691
BspLI GGNNCC 6 cut(s) 166, 1391, 1485, 1654, 2103, 2930
BspMAI CTGCAG 1 cut(s) 3576
BspMI ACCTGC 1 cut(s) 2113
BspPI GGATC 1 cut(s) 2696
BspT104I TTCGAA 1 cut(s) 3646
BspT107I GGYRCC 1 cut(s) 1389
BspTI CTTAAG 1 cut(s) 1874
BspTNI GGTCTC 1 cut(s) 2311
BsrBI CCGCTC 2 cut(s) 2524, 3213
BsrDI GCAATG 2 cut(s) 142, 2724
BsrFI RCCGGY 2 cut(s) 763, 2747
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 8 cut(s) 755, 949, 1467, 1567, 1767, 2904, 3101, 3524
BssAI RCCGGY 2 cut(s) 763, 2747
BssECI CCNNGG 3 cut(s) 1681, 3177, 3242
BssMI GATC 9 cut(s) 1096, 1266, 2399, 2545, 2598, 2688, 2871, 2980, 3259
BssT1I CCWWGG 2 cut(s) 1681, 3177
Bst2UI CCWGG 4 cut(s) 1925, 3122, 3243, 3365
Bst6I CTCTTC 2 cut(s) 95, 3472
BstAFI CTTAAG 1 cut(s) 1874
BstAPI GCANNNNNTGC 1 cut(s) 2583
BstAUI TGTACA 1 cut(s) 954
BstBAI YACGTR 1 cut(s) 2477
BstBI TTCGAA 1 cut(s) 3646
BstENI CCTNNNNNAGG 1 cut(s) 1998
BstF5I GGATG 6 cut(s) 1199, 2465, 2791, 3105, 3331, 3373
BstFNI CGCG 1 cut(s) 2936
BstHHI GCGC 1 cut(s) 2938
BstKTI GATC 9 cut(s) 1099, 1269, 2402, 2548, 2601, 2691, 2874, 2983, 3262
BstMAI GTCTC 5 cut(s) 1163, 1187, 1991, 2053, 2311
BstMBI GATC 9 cut(s) 1096, 1266, 2399, 2545, 2598, 2688, 2871, 2980, 3259
BstMCI CGRYCG 3 cut(s) 17, 296, 2748
BstNI CCWGG 4 cut(s) 1925, 3122, 3243, 3365
BstNSI RCATGY 5 cut(s) 1448, 1956, 3070, 3419, 3551
BstSCI CCNGG 6 cut(s) 1923, 2427, 3006, 3120, 3241, 3363
BstSFI CTRYAG 3 cut(s) 2833, 3572, 3665
BstSLI GKGCMC 3 cut(s) 1896, 2211, 2669
BstUI CGCG 1 cut(s) 2936
BstV1I GCAGC 9 cut(s) 171, 187, 238, 800, 1040, 1834, 2239, 2362, 2899
BstV2I GAAGAC 2 cut(s) 300, 3475
BstX2I RGATCY 2 cut(s) 2545, 3259
BstXI CCANNNNNNTGG 2 cut(s) 494, 1400
BstYI RGATCY 2 cut(s) 2545, 3259
BstZI CGGCCG 3 cut(s) 14, 293, 2745
BsuI GTATCC 2 cut(s) 173, 3353
BsuRI GGCC 8 cut(s) 16, 295, 763, 1079, 1529, 1748, 2747, 3588
BtgZI GCGATG 1 cut(s) 3289
BtsCI GGATG 6 cut(s) 1199, 2465, 2791, 3105, 3331, 3373
BtsI GCAGTG 3 cut(s) 405, 810, 2152
BtsIMutI CAGTG 7 cut(s) 405, 810, 1760, 2019, 2152, 2608, 2897
BveI ACCTGC 1 cut(s) 2113
CaiI CAGNNNCTG 6 cut(s) 1120, 1310, 2217, 2351, 3557, 3664
CciI TCATGA 2 cut(s) 2089, 2691
CfoI GCGC 1 cut(s) 2938
Cfr10I RCCGGY 2 cut(s) 763, 2747
Cfr13I GGNCC 3 cut(s) 1483, 1652, 2989
CseI GACGC 1 cut(s) 564
Csp6I GTAC 8 cut(s) 121, 955, 1619, 2233, 2563, 2764, 2965, 3137
CspCI CAANNNNNGTGG 2 cut(s) 2008, 2043
CviQI GTAC 8 cut(s) 121, 955, 1619, 2233, 2563, 2764, 2965, 3137
DpnI GATC 9 cut(s) 1098, 1268, 2401, 2547, 2600, 2690, 2873, 2982, 3261
DpnII GATC 9 cut(s) 1096, 1266, 2399, 2545, 2598, 2688, 2871, 2980, 3259
DraIII CACNNNGTG 1 cut(s) 2037
DriI GACNNNNNGTC 1 cut(s) 2893
EaeI YGGCCR 4 cut(s) 14, 293, 761, 2745
EagI CGGCCG 3 cut(s) 14, 293, 2745
Eam1104I CTCTTC 2 cut(s) 95, 3472
Eam1105I GACNNNNNGTC 1 cut(s) 2893
EarI CTCTTC 2 cut(s) 95, 3472
EciI GGCGGA 3 cut(s) 80, 121, 3062
EclXI CGGCCG 3 cut(s) 14, 293, 2745
Eco130I CCWWGG 2 cut(s) 1681, 3177
Eco147I AGGCCT 1 cut(s) 3588
Eco24I GRGCYC 1 cut(s) 1304
Eco31I GGTCTC 1 cut(s) 2311
Eco32I GATATC 3 cut(s) 457, 1969, 3406
Eco47I GGWCC 3 cut(s) 1483, 1652, 2989
Eco52I CGGCCG 3 cut(s) 14, 293, 2745
Eco57I CTGAAG 7 cut(s) 497, 962, 2272, 2343, 2969, 3117, 3459
Eco88I CYCGRG 1 cut(s) 285
EcoNI CCTNNNNNAGG 1 cut(s) 1998
EcoO109I RGGNCCY 2 cut(s) 1483, 2989
EcoRI GAATTC 1 cut(s) 3396
EcoRII CCWGG 4 cut(s) 1923, 3120, 3241, 3363
EcoRV GATATC 3 cut(s) 457, 1969, 3406
EcoT14I CCWWGG 2 cut(s) 1681, 3177
EcoT22I ATGCAT 1 cut(s) 3549
EcoT38I GRGCYC 1 cut(s) 1304
ErhI CCWWGG 2 cut(s) 1681, 3177
FaqI GGGAC 1 cut(s) 2052
FauI CCCGC 1 cut(s) 2517
FauNDI CATATG 3 cut(s) 1373, 1632, 2957
FbaI TGATCA 1 cut(s) 1266
FokI GGATG 6 cut(s) 1186, 2472, 2798, 3112, 3338, 3380
FriOI GRGCYC 1 cut(s) 1304
FspBI CTAG 3 cut(s) 969, 1545, 2736
GlaI GCGC 1 cut(s) 2937
GsaI CCCAGC 2 cut(s) 633, 3379
GsuI CTGGAG 6 cut(s) 77, 780, 3248, 3453, 3639, 3645
HaeIII GGCC 8 cut(s) 16, 295, 763, 1079, 1529, 1748, 2747, 3588
HapII CCGG 9 cut(s) 168, 764, 1549, 2428, 2713, 2748, 2783, 3008, 3080
HgaI GACGC 1 cut(s) 564
HhaI GCGC 1 cut(s) 2938
Hin6I GCGC 1 cut(s) 2936
HinP1I GCGC 1 cut(s) 2936
HincII GTYRAC 2 cut(s) 2029, 3562
HindII GTYRAC 2 cut(s) 2029, 3562
HindIII AAGCTT 1 cut(s) 2260
HpaII CCGG 9 cut(s) 168, 764, 1549, 2428, 2713, 2748, 2783, 3008, 3080
Hpy166II GTNNAC 8 cut(s) 965, 1331, 1652, 1894, 2029, 2209, 2667, 3562
Hpy8I GTNNAC 8 cut(s) 965, 1331, 1652, 1894, 2029, 2209, 2667, 3562
Hpy99I CGWCG 4 cut(s) 22, 178, 188, 580
HpyCH4IV ACGT 4 cut(s) 2476, 2533, 2762, 2859
HpySE526I ACGT 4 cut(s) 2476, 2533, 2762, 2859
HspAI GCGC 1 cut(s) 2936
KroI GCCGGC 1 cut(s) 763
KroNI GCCGGC 1 cut(s) 765
Ksp22I TGATCA 1 cut(s) 1266
Kzo9I GATC 9 cut(s) 1096, 1266, 2399, 2545, 2598, 2688, 2871, 2980, 3259
LmnI GCTCC 9 cut(s) 170, 626, 747, 799, 2830, 2952, 3001, 3434, 3620
Lsp1109I GCAGC 9 cut(s) 171, 187, 238, 800, 1040, 1834, 2239, 2362, 2899
LweI GCATC 8 cut(s) 1662, 2204, 2372, 2611, 2895, 3090, 3266, 3316
MaeI CTAG 3 cut(s) 969, 1545, 2736
MaeII ACGT 4 cut(s) 2476, 2533, 2762, 2859
MaeIII GTNAC 9 cut(s) 539, 1454, 1558, 1567, 1582, 2179, 2895, 3056, 3142
MalI GATC 9 cut(s) 1098, 1268, 2401, 2547, 2600, 2690, 2873, 2982, 3261
MbiI CCGCTC 2 cut(s) 2524, 3213
MboI GATC 9 cut(s) 1096, 1266, 2399, 2545, 2598, 2688, 2871, 2980, 3259
MfeI CAATTG 1 cut(s) 2674
MflI RGATCY 2 cut(s) 2545, 3259
MhlI GDGCHC 6 cut(s) 1304, 1494, 1896, 2211, 2669, 3161
MlyI GAGTC 6 cut(s) 197, 224, 2357, 3064, 3215, 3458
MmeI TCCRAC 2 cut(s) 2092, 3183
Mph1103I ATGCAT 1 cut(s) 3549
MroNI GCCGGC 1 cut(s) 763
MroXI GAANNNNTTC 3 cut(s) 482, 1150, 2282
MslI CAYNNNNRTG 2 cut(s) 1398, 2696
MspA1I CMGCKG 2 cut(s) 162, 229
MspCI CTTAAG 1 cut(s) 1874
MspI CCGG 9 cut(s) 168, 764, 1549, 2428, 2713, 2748, 2783, 3008, 3080
MspR9I CCNGG 6 cut(s) 1925, 2429, 3008, 3122, 3243, 3365
MunI CAATTG 1 cut(s) 2674
Mva1269I GAATGC 2 cut(s) 1907, 3189
MvaI CCWGG 4 cut(s) 1925, 3122, 3243, 3365
MvnI CGCG 1 cut(s) 2936
NaeI GCCGGC 1 cut(s) 765
NciI CCSGG 2 cut(s) 2429, 3008
NdeI CATATG 3 cut(s) 1373, 1632, 2957
NdeII GATC 9 cut(s) 1096, 1266, 2399, 2545, 2598, 2688, 2871, 2980, 3259
NgoMIV GCCGGC 1 cut(s) 763
NlaIV GGNNCC 6 cut(s) 166, 1391, 1485, 1654, 2103, 2930
NmuCI GTSAC 4 cut(s) 1454, 1558, 2895, 3056
NsiI ATGCAT 1 cut(s) 3549
NspI RCATGY 5 cut(s) 1448, 1956, 3070, 3419, 3551
NspV TTCGAA 1 cut(s) 3646
PacI TTAATTAA 1 cut(s) 2655
PaeI GCATGC 2 cut(s) 1448, 3551
PaeR7I CTCGAG 1 cut(s) 285
PagI TCATGA 2 cut(s) 2089, 2691
PceI AGGCCT 1 cut(s) 3588
PciI ACATGT 1 cut(s) 3066
PcsI WCGNNNNNNNCGW 3 cut(s) 95, 575, 2856
PctI GAATGC 2 cut(s) 1907, 3189
PdiI GCCGGC 1 cut(s) 765
PdmI GAANNNNTTC 3 cut(s) 482, 1150, 2282
Pfl23II CGTACG 1 cut(s) 2763
PflFI GACNNNGTC 1 cut(s) 574
PflMI CCANNNNNTGG 1 cut(s) 2938
PfoI TCCNGGA 2 cut(s) 1923, 3120
PleI GAGTC 6 cut(s) 196, 223, 2356, 3063, 3214, 3458
PpsI GAGTC 6 cut(s) 196, 223, 2356, 3063, 3214, 3458
Ppu21I YACGTR 1 cut(s) 2477
PpuMI RGGWCCY 2 cut(s) 1483, 2989
PscI ACATGT 1 cut(s) 3066
Psp5II RGGWCCY 2 cut(s) 1483, 2989
Psp6I CCWGG 4 cut(s) 1923, 3120, 3241, 3363
PspFI CCCAGC 2 cut(s) 629, 3375
PspGI CCWGG 4 cut(s) 1923, 3120, 3241, 3363
PspLI CGTACG 1 cut(s) 2763
PspN4I GGNNCC 6 cut(s) 166, 1391, 1485, 1654, 2103, 2930
PspPI GGNCC 3 cut(s) 1483, 1652, 2989
PspPPI RGGWCCY 2 cut(s) 1483, 2989
PspXI VCTCGAGB 1 cut(s) 285
PsrI GAACNNNNNNTAC 2 cut(s) 1782, 1814
PstI CTGCAG 1 cut(s) 3576
PstNI CAGNNNCTG 6 cut(s) 1120, 1310, 2217, 2351, 3557, 3664
PsuI RGATCY 2 cut(s) 2545, 3259
PsyI GACNNNGTC 1 cut(s) 574
RsaI GTAC 8 cut(s) 122, 956, 1620, 2234, 2564, 2765, 2966, 3138
RsaNI GTAC 8 cut(s) 121, 955, 1619, 2233, 2563, 2764, 2965, 3137
RseI CAYNNNNRTG 2 cut(s) 1398, 2696
Sau3AI GATC 9 cut(s) 1096, 1266, 2399, 2545, 2598, 2688, 2871, 2980, 3259
Sau96I GGNCC 3 cut(s) 1483, 1652, 2989
ScaI AGTACT 1 cut(s) 3138
SchI GAGTC 6 cut(s) 197, 224, 2357, 3064, 3215, 3458
ScrFI CCNGG 6 cut(s) 1925, 2429, 3008, 3122, 3243, 3365
SduI GDGCHC 6 cut(s) 1304, 1494, 1896, 2211, 2669, 3161
SfaNI GCATC 8 cut(s) 1662, 2204, 2372, 2611, 2895, 3090, 3266, 3316
SfcI CTRYAG 3 cut(s) 2833, 3572, 3665
Sfr274I CTCGAG 1 cut(s) 285
SfuI TTCGAA 1 cut(s) 3646
SinI GGWCC 3 cut(s) 1483, 1652, 2989
SlaI CTCGAG 1 cut(s) 285
SmiMI CAYNNNNRTG 2 cut(s) 1398, 2696
SmlI CTYRAG 5 cut(s) 285, 926, 1874, 3349, 3491
SmoI CTYRAG 5 cut(s) 285, 926, 1874, 3349, 3491
SpeI ACTAGT 1 cut(s) 2735
SphI GCATGC 2 cut(s) 1448, 3551
SseBI AGGCCT 1 cut(s) 3588
SspI AATATT 4 cut(s) 491, 1149, 1364, 2485
SspMI CTAG 3 cut(s) 969, 1545, 2736
StuI AGGCCT 1 cut(s) 3588
StyD4I CCNGG 6 cut(s) 1923, 2427, 3006, 3120, 3241, 3363
StyI CCWWGG 2 cut(s) 1681, 3177
TaiI ACGT 4 cut(s) 2479, 2536, 2765, 2862
TatI WGTACW 2 cut(s) 954, 3136
TauI GCSGC 8 cut(s) 16, 165, 298, 648, 703, 1861, 3014, 3216
TscAI CASTG 7 cut(s) 405, 810, 1767, 2026, 2159, 2608, 2904
TseFI GTSAC 4 cut(s) 1454, 1558, 2895, 3056
Tsp45I GTSAC 4 cut(s) 1454, 1558, 2895, 3056
TspGWI ACGGA 3 cut(s) 900, 2882, 2904
TspRI CASTG 7 cut(s) 405, 810, 1767, 2026, 2159, 2608, 2904
Tth111I GACNNNGTC 1 cut(s) 574
Van91I CCANNNNNTGG 1 cut(s) 2938
Vha464I CTTAAG 1 cut(s) 1874
VneI GTGCAC 3 cut(s) 1892, 2207, 2665
VpaK11BI GGWCC 3 cut(s) 1483, 1652, 2989
XagI CCTNNNNNAGG 1 cut(s) 1998
XceI RCATGY 5 cut(s) 1448, 1956, 3070, 3419, 3551
XcmI CCANNNNNNNNNTGG 3 cut(s) 1400, 1904, 2165
XhoI CTCGAG 1 cut(s) 285
XmnI GAANNNNTTC 3 cut(s) 482, 1150, 2282
XspI CTAG 3 cut(s) 969, 1545, 2736
ZrmI AGTACT 1 cut(s) 3138
Zsp2I ATGCAT 1 cut(s) 3549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.