AT3G62140

N-terminal domain of NEFA-interacting nuclear protein NIP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005793
Physical Location & Seq
Forward (+)
23007083 .. 23008749
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62140.1

Sequence Viewer

Length: 645 bp
ATGGTTGATGAGACAGAAAAACCTATTAGGTTACTCAATTTCGTCTCCGAGGAACAGTTAGATGAATCGAAGAAAGAAAGAGGAGAAAGAGTTGAAGATGGAACATTCCAGAGGGACAGGGCTCTCTACGAGATTTTGAAAGAGAACAAAGACAAGAAGGACGCTGAGTTTAATGAACGATTCAAGCATAGGCCACCCAAAGCTCTGGATGAAGATGAGACCGAGTTTCTTGATAAACTGGAAATGTCAAAGAGGGAATATGAACGTCAATTGGCAAATGAAGAAGATGAACAGCTTCGTAATTTCCAGGCAGCGGTTGCAGCACGATCTGCAATCCTTCACGAACCAAAGGAAGCAGCACTGCCACCACCAGCGCCAGTCACCAAGGAACAAAAACCAATCGGGAAGAGAAATCCTGCGACTCGTCCATTTAAAGCGATCATAAAAGTCAAGCCACAACCCAAGAAGGCTAAAGCAACAGAGAAAGAAGAGAAAGAGATCCCAGGGAATGGAAAACCGGCCAGCCATATCGACCAGGCTTCTTTAGATTCTGTAAAAGGCAATGTCACAGGTAAAACTACTGAAGGTCTGCAAACTGGTTTAGCCTTAGTCTCATATAGTGATGAAAGTGAAGACGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nefa_Nip30_N

Protein Analysis

214

Amino Acids

24.29

Weight (kDa)

5.24

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM192A_Fyv6_N PF10187 18 - 103 2.1e-15 FAM192A/Fyv6, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 509
AciI CCGC 1 cut(s) 314
AclWI GGATC 1 cut(s) 493
AcoI YGGCCR 1 cut(s) 519
AcuI CTGAAG 1 cut(s) 603
AfiI CCNNNNNNNGG 2 cut(s) 313, 509
AgsI TTSAA 3 cut(s) 95, 139, 184
AjnI CCWGG 3 cut(s) 306, 502, 534
AluBI AGCT 2 cut(s) 203, 295
AluI AGCT 2 cut(s) 203, 295
Alw26I GTCTC 4 cut(s) 5, 49, 212, 616
AlwI GGATC 1 cut(s) 493
AoxI GGCC 2 cut(s) 191, 519
ApeKI GCWGC 3 cut(s) 311, 320, 356
Asp700I GAANNNNTTC 1 cut(s) 294
AspLEI GCGC 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 373
BanII GRGCYC 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 639
BbvI GCAGC 3 cut(s) 323, 332, 368
BccI CCATC 1 cut(s) 92
BciT130I CCWGG 3 cut(s) 308, 504, 536
BcoDI GTCTC 4 cut(s) 5, 49, 212, 616
BfoI RGCGCY 1 cut(s) 377
BisI GCNGC 3 cut(s) 312, 321, 357
BlsI GCNGC 3 cut(s) 313, 322, 358
Bme1390I CCNGG 3 cut(s) 308, 504, 536
BmrFI CCNGG 3 cut(s) 308, 504, 536
BpiI GAAGAC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 4 cut(s) 48, 384, 502, 503
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 509
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 3 cut(s) 243, 377, 601
Bse3DI GCAATG 1 cut(s) 568
BseBI CCWGG 3 cut(s) 308, 504, 536
BseDI CCNNGG 4 cut(s) 48, 384, 502, 503
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 313, 509
BseMI GCAATG 1 cut(s) 568
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 3 cut(s) 243, 377, 601
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 3 cut(s) 323, 332, 368
BshFI GGCC 2 cut(s) 193, 521
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 313, 509
BsmAI GTCTC 4 cut(s) 5, 49, 212, 616
BsmBI CGTCTC 1 cut(s) 49
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 2 cut(s) 193, 521
Bso31I GGTCTC 1 cut(s) 212
Bsp1286I GDGCHC 1 cut(s) 124
Bsp143I GATC 3 cut(s) 326, 438, 498
BspACI CCGC 1 cut(s) 314
BspANI GGCC 2 cut(s) 193, 521
BspCNI CTCAG 1 cut(s) 157
BspPI GGATC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 568
BsrFI RCCGGY 1 cut(s) 517
BsrI ACTGG 3 cut(s) 243, 377, 601
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 4 cut(s) 48, 384, 502, 503
BssMI GATC 3 cut(s) 326, 438, 498
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 3 cut(s) 308, 504, 536
Bst4CI ACNGT 1 cut(s) 57
Bst6I CTCTTC 2 cut(s) 401, 483
BstAPI GCANNNNNTGC 2 cut(s) 317, 329
BstC8I GCNNGC 1 cut(s) 523
BstDEI CTNAG 2 cut(s) 165, 607
BstF5I GGATG 1 cut(s) 214
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 3 cut(s) 329, 441, 501
BstMAI GTCTC 4 cut(s) 5, 49, 212, 616
BstMBI GATC 3 cut(s) 326, 438, 498
BstMWI GCNNNNNNNGC 3 cut(s) 317, 320, 329
BstNI CCWGG 3 cut(s) 308, 504, 536
BstSCI CCNGG 3 cut(s) 306, 502, 534
BstV1I GCAGC 3 cut(s) 323, 332, 368
BstV2I GAAGAC 1 cut(s) 639
BstX2I RGATCY 1 cut(s) 498
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 1 cut(s) 498
BsuRI GGCC 2 cut(s) 193, 521
BtsCI GGATG 1 cut(s) 214
BtsI GCAGTG 1 cut(s) 359
BtsIMutI CAGTG 1 cut(s) 359
Cac8I GCNNGC 1 cut(s) 523
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 517
CseI GACGC 1 cut(s) 170
DdeI CTNAG 2 cut(s) 165, 607
DpnI GATC 3 cut(s) 328, 440, 500
DpnII GATC 3 cut(s) 326, 438, 498
DraI TTTAAA 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 2 cut(s) 401, 483
EarI CTCTTC 2 cut(s) 401, 483
Eco130I CCWWGG 1 cut(s) 384
Eco24I GRGCYC 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 603
EcoRII CCWGG 3 cut(s) 306, 502, 534
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 49
FaiI YATR 6 cut(s) 189, 261, 443, 528, 616, 618
FaqI GGGAC 1 cut(s) 128
Fnu4HI GCNGC 3 cut(s) 312, 321, 357
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 124
Fsp4HI GCNGC 3 cut(s) 312, 321, 357
GlaI GCGC 1 cut(s) 375
GluI GCNGC 3 cut(s) 312, 321, 357
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 2 cut(s) 193, 521
HapII CCGG 1 cut(s) 518
HgaI GACGC 1 cut(s) 170
HhaI GCGC 1 cut(s) 376
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HinfI GANTC 4 cut(s) 65, 180, 421, 548
HpaII CCGG 1 cut(s) 518
HphI GGTGA 1 cut(s) 373
Hpy188I TCNGA 1 cut(s) 49
Hpy188III TCNNGA 5 cut(s) 109, 206, 230, 341, 403
HpyAV CCTTC 4 cut(s) 151, 347, 460, 578
HpyCH4III ACNGT 1 cut(s) 57
HpyCH4IV ACGT 1 cut(s) 265
HpyCH4V TGCA 3 cut(s) 320, 332, 592
HpyF10VI GCNNNNNNNGC 3 cut(s) 317, 320, 329
HpyF3I CTNAG 2 cut(s) 165, 607
HpySE526I ACGT 1 cut(s) 265
HspAI GCGC 1 cut(s) 374
Kzo9I GATC 3 cut(s) 326, 438, 498
Lsp1109I GCAGC 3 cut(s) 323, 332, 368
MaeII ACGT 1 cut(s) 265
MaeIII GTNAC 3 cut(s) 30, 379, 565
MalI GATC 3 cut(s) 328, 440, 500
MboI GATC 3 cut(s) 326, 438, 498
MboII GAAGA 8 cut(s) 82, 107, 224, 293, 296, 418, 500, 644
MfeI CAATTG 1 cut(s) 269
MflI RGATCY 1 cut(s) 498
MhlI GDGCHC 1 cut(s) 124
MluCI AATT 3 cut(s) 37, 269, 301
MlyI GAGTC 1 cut(s) 415
MnlI CCTC 4 cut(s) 43, 74, 105, 246
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 3 cut(s) 171, 432, 643
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 3 cut(s) 308, 504, 536
MunI CAATTG 1 cut(s) 269
MvaI CCWGG 3 cut(s) 308, 504, 536
MwoI GCNNNNNNNGC 3 cut(s) 317, 320, 329
NdeII GATC 3 cut(s) 326, 438, 498
NmuCI GTSAC 2 cut(s) 379, 565
PasI CCCWGGG 1 cut(s) 503
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 3 cut(s) 65, 180, 548
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 3 cut(s) 313, 322, 358
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 306, 502, 534
PspGI CCWGG 3 cut(s) 306, 502, 534
PsuI RGATCY 1 cut(s) 498
SaqAI TTAA 3 cut(s) 171, 432, 643
SatI GCNGC 3 cut(s) 312, 321, 357
Sau3AI GATC 3 cut(s) 326, 438, 498
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 3 cut(s) 308, 504, 536
SduI GDGCHC 1 cut(s) 124
SetI ASST 7 cut(s) 25, 32, 205, 268, 297, 574, 589
Sse9I AATT 3 cut(s) 37, 269, 301
SsiI CCGC 1 cut(s) 314
StyD4I CCNGG 3 cut(s) 306, 502, 534
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 1 cut(s) 57
TaiI ACGT 1 cut(s) 268
TaqI TCGA 2 cut(s) 68, 531
TaqII GACCGA 1 cut(s) 236
TasI AATT 3 cut(s) 37, 269, 301
TfiI GAWTC 3 cut(s) 65, 180, 548
Tru1I TTAA 3 cut(s) 171, 432, 643
Tru9I TTAA 3 cut(s) 171, 432, 643
TscAI CASTG 1 cut(s) 366
TseFI GTSAC 2 cut(s) 379, 565
TseI GCWGC 3 cut(s) 311, 320, 356
Tsp45I GTSAC 2 cut(s) 379, 565
TspDTI ATGAA 7 cut(s) 78, 189, 225, 276, 294, 303, 639
TspRI CASTG 1 cut(s) 366
Van91I CCANNNNNTGG 1 cut(s) 509
XmnI GAANNNNTTC 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.