Rw3G024150

N-terminal domain of NEFA-interacting nuclear protein NIP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005793
Physical Location & Seq
Forward (+)
30813980 .. 30816076
2097 bp
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UTR
Exon/CDS
Intron
Rw3G024150.1

Sequence Viewer

Length: 633 bp
ATGGCTGAAGATTTCACACGCCCGCGGCTCATGAATTTCGTTTCTGAGGAACAGTTAGATGAAGCCAAGAAAACCCGGGGTGAACGAGTCGAGGATGGCACTGCCTACCGGGACAGACCCCTTTTCGAGATTCTGAAAGAAAACAAGGACAAGAAGGATGCGGAGTTCAATGAACGTTTCAAGCACAGACCGCCGAAAGCTTTGGACGATGACGAGACGGAGTTTCTTGATCAATGGGAGACCTCAAAGAAGGAATATGAACGACAGGTTGCAGATGAGGAGGCCCAACAACTTTTGAGTTTTCAGGCAGCGGTGGCAGCAAAATCTAGCATTGTACAAGAGTTGACGGAAATACCTTCTGTTCCGGTAGTCAAGCAGCAGAAACCAGCCGGGAAGAGGAATCCACCTACTTTTCCATTAGGCATGATTGTTAAAGTGAAGCCACAGGCGAAGAAAGCTAAAATTGATCAAGGGGGCATTGAAGAAGCTACAAGCACAGCGAAAACCCCTTATGTTGATACAGATAAAACTTTAGAGGTAACCAAGACACCTGAGGCTGATACTGATAAGTCCAGTGTTGTTTCTAAGACTGGTCTTGTTTCATACAGTGATGATAGTGAAGATGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nefa_Nip30_N

Protein Analysis

210

Amino Acids

23.73

Weight (kDa)

4.88

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM192A_Fyv6_N PF10187 17 - 101 1e-16 FAM192A/Fyv6, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 25
AciI CCGC 5 cut(s) 23, 25, 161, 191, 311
AclI AACGTT 1 cut(s) 175
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 396
AgsI TTSAA 3 cut(s) 169, 181, 482
AloI GAACNNNNNNTCC 2 cut(s) 149, 181
AluBI AGCT 3 cut(s) 200, 458, 488
AluI AGCT 3 cut(s) 200, 458, 488
Alw26I GTCTC 2 cut(s) 209, 233
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 3 cut(s) 308, 317, 376
ApoI RAATTY 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 283
AsuC2I CCSGG 4 cut(s) 76, 77, 110, 391
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 75
AxyI CCTNAGG 1 cut(s) 552
BbvI GCAGC 3 cut(s) 320, 329, 388
BccI CCATC 1 cut(s) 89
BclI TGATCA 2 cut(s) 229, 466
BcnI CCSGG 4 cut(s) 76, 77, 110, 391
BcoDI GTCTC 2 cut(s) 209, 233
BfaI CTAG 1 cut(s) 327
BisI GCNGC 4 cut(s) 26, 309, 318, 377
BlsI GCNGC 4 cut(s) 27, 310, 319, 378
Bme1390I CCNGG 4 cut(s) 76, 77, 110, 391
BmeT110I CYCGRG 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 283
BmrFI CCNGG 4 cut(s) 76, 77, 110, 391
BmsI GCATC 1 cut(s) 148
BpuMI CCSGG 4 cut(s) 76, 77, 110, 391
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 3 cut(s) 23, 75, 76
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 1 cut(s) 396
Bse1I ACTGG 2 cut(s) 573, 595
Bse21I CCTNAGG 1 cut(s) 552
BseDI CCNNGG 3 cut(s) 23, 75, 76
BseGI GGATG 2 cut(s) 100, 163
BseLI CCNNNNNNNGG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 36, 543
BseNI ACTGG 2 cut(s) 573, 595
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 3 cut(s) 320, 329, 388
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 4 cut(s) 76, 109, 365, 390
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 396
BsmAI GTCTC 2 cut(s) 209, 233
BsmBI CGTCTC 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 1 cut(s) 284
Bso31I GGTCTC 1 cut(s) 233
BsoBI CYCGRG 1 cut(s) 75
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 2 cut(s) 229, 466
BspACI CCGC 5 cut(s) 23, 25, 161, 191, 311
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 2 cut(s) 37, 544
BspFNI CGCG 1 cut(s) 25
BspHI TCATGA 1 cut(s) 30
BspTNI GGTCTC 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 2 cut(s) 573, 595
BssECI CCNNGG 3 cut(s) 23, 75, 76
BssMI GATC 2 cut(s) 229, 466
Bst4CI ACNGT 2 cut(s) 54, 608
Bst6I CTCTTC 1 cut(s) 389
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 3 cut(s) 45, 552, 585
BstDSI CCRYGG 1 cut(s) 23
BstEII GGTNACC 1 cut(s) 538
BstF5I GGATG 2 cut(s) 100, 163
BstFNI CGCG 1 cut(s) 25
BstKTI GATC 2 cut(s) 232, 469
BstMAI GTCTC 2 cut(s) 209, 233
BstMBI GATC 2 cut(s) 229, 466
BstMWI GCNNNNNNNGC 4 cut(s) 190, 314, 317, 455
BstPI GGTNACC 1 cut(s) 538
BstSCI CCNGG 4 cut(s) 74, 75, 108, 389
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 3 cut(s) 320, 329, 388
Bsu36I CCTNAGG 1 cut(s) 552
BsuRI GGCC 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 23
BtsCI GGATG 2 cut(s) 100, 163
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 99, 580, 613
Cac8I GCNNGC 1 cut(s) 23
CciI TCATGA 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 283
Cfr42I CCGCGG 1 cut(s) 26
Cfr9I CCCGGG 1 cut(s) 75
Csp6I GTAC 1 cut(s) 335
CviAII CATG 2 cut(s) 31, 424
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 3 cut(s) 45, 552, 585
DpnI GATC 2 cut(s) 231, 468
DpnII GATC 2 cut(s) 229, 466
Eam1104I CTCTTC 1 cut(s) 389
EarI CTCTTC 1 cut(s) 389
Eco31I GGTCTC 1 cut(s) 233
Eco57I CTGAAG 1 cut(s) 27
Eco81I CCTNAGG 1 cut(s) 552
Eco88I CYCGRG 1 cut(s) 75
Eco91I GGTNACC 1 cut(s) 538
EcoO65I GGTNACC 1 cut(s) 538
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 2 cut(s) 34, 427
FaiI YATR 5 cut(s) 32, 258, 425, 513, 604
FaqI GGGAC 1 cut(s) 125
FatI CATG 2 cut(s) 30, 423
FauI CCCGC 1 cut(s) 30
FbaI TGATCA 2 cut(s) 229, 466
Fnu4HI GCNGC 4 cut(s) 26, 309, 318, 377
FokI GGATG 2 cut(s) 107, 170
Fsp4HI GCNGC 4 cut(s) 26, 309, 318, 377
FspBI CTAG 1 cut(s) 327
GluI GCNGC 4 cut(s) 26, 309, 318, 377
HaeIII GGCC 1 cut(s) 284
HapII CCGG 4 cut(s) 76, 109, 365, 390
Hin1II CATG 2 cut(s) 34, 427
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 3 cut(s) 87, 130, 400
HpaII CCGG 4 cut(s) 76, 109, 365, 390
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 2 cut(s) 83, 345
Hpy188I TCNGA 2 cut(s) 46, 135
Hpy188III TCNNGA 3 cut(s) 31, 127, 227
Hpy8I GTNNAC 2 cut(s) 83, 345
HpyAV CCTTC 3 cut(s) 148, 244, 366
HpyCH4III ACNGT 2 cut(s) 54, 608
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 1 cut(s) 272
HpyF10VI GCNNNNNNNGC 4 cut(s) 190, 314, 317, 455
HpyF3I CTNAG 3 cut(s) 45, 552, 585
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 2 cut(s) 34, 427
Ksp22I TGATCA 2 cut(s) 229, 466
KspI CCGCGG 1 cut(s) 26
Kzo9I GATC 2 cut(s) 229, 466
Lsp1109I GCAGC 3 cut(s) 320, 329, 388
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 327
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 538
MalI GATC 2 cut(s) 231, 468
MboI GATC 2 cut(s) 229, 466
MboII GAAGA 5 cut(s) 20, 406, 463, 494, 632
MluCI AATT 2 cut(s) 34, 462
MlyI GAGTC 1 cut(s) 96
MnlI CCTC 8 cut(s) 40, 85, 253, 271, 274, 390, 529, 547
MseI TTAA 1 cut(s) 432
MspA1I CMGCKG 2 cut(s) 25, 311
MspI CCGG 4 cut(s) 76, 109, 365, 390
MspR9I CCNGG 4 cut(s) 76, 77, 110, 391
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 4 cut(s) 190, 314, 317, 455
NciI CCSGG 4 cut(s) 76, 77, 110, 391
NdeII GATC 2 cut(s) 229, 466
NlaIII CATG 2 cut(s) 34, 427
PagI TCATGA 1 cut(s) 30
PfeI GAWTC 2 cut(s) 130, 400
PkrI GCNGC 4 cut(s) 27, 310, 319, 378
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp1406I AACGTT 1 cut(s) 175
PspEI GGTNACC 1 cut(s) 538
PspPI GGNCC 1 cut(s) 283
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
SacII CCGCGG 1 cut(s) 26
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 4 cut(s) 26, 309, 318, 377
Sau3AI GATC 2 cut(s) 229, 466
Sau96I GGNCC 1 cut(s) 283
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 4 cut(s) 76, 77, 110, 391
SfaNI GCATC 1 cut(s) 148
Sfr303I CCGCGG 1 cut(s) 26
SgrBI CCGCGG 1 cut(s) 26
SmaI CCCGGG 1 cut(s) 77
Sse9I AATT 2 cut(s) 34, 462
SsiI CCGC 5 cut(s) 23, 25, 161, 191, 311
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 4 cut(s) 74, 75, 108, 389
TaaI ACNGT 2 cut(s) 54, 608
TaiI ACGT 1 cut(s) 178
TaqI TCGA 2 cut(s) 90, 126
TasI AATT 2 cut(s) 34, 462
TatI WGTACW 1 cut(s) 334
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 2 cut(s) 130, 400
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 3 cut(s) 106, 580, 613
TseI GCWGC 3 cut(s) 308, 317, 376
TspDTI ATGAA 5 cut(s) 47, 75, 186, 273, 591
TspGWI ACGGA 2 cut(s) 233, 362
TspMI CCCGGG 1 cut(s) 75
TspRI CASTG 3 cut(s) 106, 580, 613
XapI RAATTY 1 cut(s) 34
XmaI CCCGGG 1 cut(s) 75
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.