MD17G1284800.v1.1

N-terminal domain of NEFA-interacting nuclear protein NIP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005793
Physical Location & Seq
Reverse (-)
34407850 .. 34410624
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1284800.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGCCGAGGACGATTTCACACGCCCACCCCGGCTTATGAGCTTCGTCTCCGAGGAACAGTTGGATGAAGCCAAGAGAGCAAGGGGCGAACGAGTTGAGGATGGCACTGCCCAGAGAGACAGACCCCTTTTCGAGATTTTGAAAGAAAACAAAGACAAGAAGGATGCAGAGTTCAATGAACGTTTCAAGCACAGACCGCCCAAAGCTTTGGACGATGACGAGACTGAGTTCCTTGAAAATTTAGAGACTTCAAGGAAGGAATATGAGCAAAAAATTGCAGATGAGGAAGCTCAACAGCTTCTGAGTTTCCAGGCTGCGGTGGCGGCGAAGTCTAGCATTGTGCAAGAGCTTAAGGAAGCACCTTCGGTTCCTATAGGCCAGGAACAGAAATCAGCTGAGAAGAAGAATCCACCTCCTCGTCGATTAGGTATGATTATTAAGGTGCAGCCACAAGCAAAGAAAGCCAAAATTGATCGGGGAGGTAGTGAAGAACCTAGAAACACAATGAAAAGCCCACATGTAGATACAGAAAAAACTTTAGAGGTAATTAAGACGCTTAATGGTGGTATTGATAAGTCCAGTGTTGCTGCCAAGACAGGTCTTGTTTCCTATAGTGATGACAGTGAAGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nefa_Nip30_N

Protein Analysis

212

Amino Acids

23.84

Weight (kDa)

5.14

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM192A_Fyv6_N PF10187 19 - 103 3.7e-15 FAM192A/Fyv6, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 197, 317, 323
AclI AACGTT 1 cut(s) 181
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 316
AflII CTTAAG 1 cut(s) 350
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 5 cut(s) 142, 175, 187, 236, 252
AjnI CCWGG 2 cut(s) 309, 378
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 6 cut(s) 42, 206, 290, 298, 349, 395
AluI AGCT 6 cut(s) 42, 206, 290, 298, 349, 395
Alw26I GTCTC 4 cut(s) 52, 111, 215, 239
AlwNI CAGNNNCTG 1 cut(s) 301
AoxI GGCC 2 cut(s) 3, 376
ApeKI GCWGC 3 cut(s) 314, 445, 587
ApoI RAATTY 1 cut(s) 238
AsuC2I CCSGG 1 cut(s) 31
BbvI GCAGC 3 cut(s) 301, 457, 574
BccI CCATC 1 cut(s) 95
BciT130I CCWGG 2 cut(s) 311, 380
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 4 cut(s) 52, 111, 215, 239
BfaI CTAG 2 cut(s) 333, 495
BfmI CTRYAG 2 cut(s) 372, 610
BfrI CTTAAG 1 cut(s) 350
BisI GCNGC 4 cut(s) 315, 324, 446, 588
BlsI GCNGC 4 cut(s) 316, 325, 447, 589
Bme1390I CCNGG 3 cut(s) 31, 311, 380
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 3 cut(s) 31, 311, 380
BmsI GCATC 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 31
BsaJI CCNNGG 3 cut(s) 6, 29, 51
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 579
BseBI CCWGG 2 cut(s) 311, 380
BseDI CCNNGG 3 cut(s) 6, 29, 51
BseGI GGATG 3 cut(s) 70, 106, 169
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 3 cut(s) 216, 293, 387
BseNI ACTGG 1 cut(s) 579
BseRI GAGGAG 1 cut(s) 405
BseXI GCAGC 3 cut(s) 301, 457, 574
BsgI GTGCAG 1 cut(s) 464
BshFI GGCC 2 cut(s) 5, 378
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 4 cut(s) 52, 111, 215, 239
BsmBI CGTCTC 1 cut(s) 52
BsnI GGCC 2 cut(s) 5, 378
Bsp143I GATC 1 cut(s) 472
BspACI CCGC 3 cut(s) 197, 317, 323
BspANI GGCC 2 cut(s) 5, 378
BspCNI CTCAG 3 cut(s) 217, 294, 388
BspLI GGNNCC 1 cut(s) 369
BspTI CTTAAG 1 cut(s) 350
BsrI ACTGG 1 cut(s) 579
BssECI CCNNGG 3 cut(s) 6, 29, 51
BssMI GATC 1 cut(s) 472
Bst2UI CCWGG 2 cut(s) 311, 380
Bst4CI ACNGT 2 cut(s) 60, 623
BstAFI CTTAAG 1 cut(s) 350
BstDEI CTNAG 3 cut(s) 225, 302, 396
BstF5I GGATG 3 cut(s) 70, 106, 169
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 4 cut(s) 52, 111, 215, 239
BstMBI GATC 1 cut(s) 472
BstMWI GCNNNNNNNGC 5 cut(s) 77, 196, 320, 323, 461
BstNI CCWGG 2 cut(s) 311, 380
BstNSI RCATGY 1 cut(s) 521
BstSCI CCNGG 3 cut(s) 29, 309, 378
BstSFI CTRYAG 2 cut(s) 372, 610
BstV1I GCAGC 3 cut(s) 301, 457, 574
BstXI CCANNNNNNTGG 1 cut(s) 208
BsuRI GGCC 2 cut(s) 5, 378
BtsCI GGATG 3 cut(s) 70, 106, 169
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 3 cut(s) 105, 586, 628
CaiI CAGNNNCTG 1 cut(s) 301
CseI GACGC 1 cut(s) 562
CviAII CATG 1 cut(s) 518
DdeI CTNAG 3 cut(s) 225, 302, 396
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 3
EcoRII CCWGG 2 cut(s) 309, 378
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 1 cut(s) 521
FaiI YATR 6 cut(s) 38, 264, 374, 431, 519, 612
FatI CATG 1 cut(s) 517
Fnu4HI GCNGC 4 cut(s) 315, 324, 446, 588
FokI GGATG 3 cut(s) 77, 113, 176
Fsp4HI GCNGC 4 cut(s) 315, 324, 446, 588
FspBI CTAG 2 cut(s) 333, 495
GluI GCNGC 4 cut(s) 315, 324, 446, 588
HaeIII GGCC 2 cut(s) 5, 378
HapII CCGG 1 cut(s) 31
HgaI GACGC 1 cut(s) 562
Hin1II CATG 1 cut(s) 521
HindIII AAGCTT 1 cut(s) 204
HinfI GANTC 1 cut(s) 406
HpaII CCGG 1 cut(s) 31
Hpy188I TCNGA 2 cut(s) 52, 303
Hpy188III TCNNGA 1 cut(s) 133
Hpy99I CGWCG 1 cut(s) 423
HpyAV CCTTC 3 cut(s) 154, 250, 372
HpyCH4III ACNGT 2 cut(s) 60, 623
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 4 cut(s) 167, 278, 343, 445
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 196, 320, 323, 461
HpyF3I CTNAG 3 cut(s) 225, 302, 396
HpySE526I ACGT 1 cut(s) 181
Hsp92II CATG 1 cut(s) 521
Kzo9I GATC 1 cut(s) 472
LpnPI CCDG 8 cut(s) 44, 125, 296, 323, 365, 392, 582, 592
Lsp1109I GCAGC 3 cut(s) 301, 457, 574
LweI GCATC 1 cut(s) 154
MaeI CTAG 2 cut(s) 333, 495
MaeII ACGT 1 cut(s) 181
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 3 cut(s) 412, 415, 500
MluCI AATT 4 cut(s) 238, 273, 468, 546
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 7 cut(s) 46, 91, 277, 423, 426, 473, 535
MseI TTAA 4 cut(s) 351, 438, 549, 558
MspA1I CMGCKG 1 cut(s) 395
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 3 cut(s) 31, 311, 380
MvaI CCWGG 2 cut(s) 311, 380
MwoI GCNNNNNNNGC 5 cut(s) 77, 196, 320, 323, 461
NciI CCSGG 1 cut(s) 31
NdeII GATC 1 cut(s) 472
NlaIII CATG 1 cut(s) 521
NlaIV GGNNCC 1 cut(s) 369
NmeAIII GCCGAG 1 cut(s) 31
NspI RCATGY 1 cut(s) 521
PciI ACATGT 1 cut(s) 517
PfeI GAWTC 1 cut(s) 406
PkrI GCNGC 4 cut(s) 316, 325, 447, 589
PscI ACATGT 1 cut(s) 517
Psp1406I AACGTT 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 309, 378
PspGI CCWGG 2 cut(s) 309, 378
PspN4I GGNNCC 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 395
SaqAI TTAA 4 cut(s) 351, 438, 549, 558
SatI GCNGC 4 cut(s) 315, 324, 446, 588
Sau3AI GATC 1 cut(s) 472
ScrFI CCNGG 3 cut(s) 31, 311, 380
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 2 cut(s) 372, 610
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 4 cut(s) 238, 273, 468, 546
SsiI CCGC 3 cut(s) 197, 317, 323
SspMI CTAG 2 cut(s) 333, 495
StyD4I CCNGG 3 cut(s) 29, 309, 378
TaaI ACNGT 2 cut(s) 60, 623
TaiI ACGT 1 cut(s) 184
TaqI TCGA 2 cut(s) 132, 421
TasI AATT 4 cut(s) 238, 273, 468, 546
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 4 cut(s) 351, 438, 549, 558
Tru9I TTAA 4 cut(s) 351, 438, 549, 558
TscAI CASTG 3 cut(s) 112, 586, 628
TseI GCWGC 3 cut(s) 314, 445, 587
TspDTI ATGAA 3 cut(s) 81, 192, 521
TspRI CASTG 3 cut(s) 112, 586, 628
Vha464I CTTAAG 1 cut(s) 350
XapI RAATTY 1 cut(s) 238
XceI RCATGY 1 cut(s) 521
XspI CTAG 2 cut(s) 333, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.