Rh3BG306900

N-terminal domain of NEFA-interacting nuclear protein NIP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0005793
Physical Location & Seq
Forward (+)
30981067 .. 30983161
2095 bp
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UTR
Exon/CDS
Intron
Rh3BG306900.1

Sequence Viewer

Length: 633 bp
ATGGCTGAAGATTTCACACGCCCGCGGCTCATGAATTTCGTTTCTGAGGAACAGTTAGATGAAGCCAAGAAAACCCGGGGTGAACGAGTCGAGGATGGCACTGCCTACCGGGACAGACCCCTTTTCGAGATTTTGAAAGAAAACAAGGACAAGAAGGATGCGGAGTTCAATGAGCGTTTCAAGCACAGACCGCCGAAAGCTTTGGACGATGACGAGACGGAGTTTCTTGATCAATGGGAGACTTCAAAGAAGGAATATGAACGACAGGTTGCAGATGAGGAGGCCCAACAACTTTTGAGTTTTCAGGCAGCGGTGGCAGCAAAGTCTAGCATTGTACAAGAGTTGACGGAAATACCTTCTGTTCCGGTAGTCAAGGAGCAGCAGAAACCAGCCGGGAAGAGGAATCCACCTGCTTTTCCATTAGGTATGATTGTTAAAGTGAAGCCACAAACGAAGAAAGCTAAAATTGATCAAGGGGGCATTGAAGAAGCTACAAGCACAGCGAAAACCCCTTATGTTGATACAGATAAAACTTTAGAGGTAAACAAGACACCTGAGGCTGATACTGATAAGTCCAGTGTTGTTTCTAAGACTGGTCTTGTTTCATACAGTGATGATAGTGAAGATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nefa_Nip30_N

Protein Analysis

210

Amino Acids

23.76

Weight (kDa)

4.86

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM192A_Fyv6_N PF10187 17 - 101 1e-16 FAM192A/Fyv6, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 418
Acc36I ACCTGC 1 cut(s) 418
AccII CGCG 1 cut(s) 25
AciI CCGC 5 cut(s) 23, 25, 161, 191, 311
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 27
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 399
AgsI TTSAA 5 cut(s) 136, 169, 181, 246, 485
AloI GAACNNNNNNTCC 2 cut(s) 149, 181
AluBI AGCT 3 cut(s) 200, 461, 491
AluI AGCT 3 cut(s) 200, 461, 491
Alw26I GTCTC 2 cut(s) 209, 233
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 3 cut(s) 308, 317, 379
ApoI RAATTY 1 cut(s) 34
AspS9I GGNCC 1 cut(s) 283
AsuC2I CCSGG 4 cut(s) 76, 77, 110, 394
AsuHPI GGTGA 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 75
AxyI CCTNAGG 1 cut(s) 555
BbvI GCAGC 3 cut(s) 320, 329, 391
BccI CCATC 1 cut(s) 89
BclI TGATCA 2 cut(s) 229, 469
BcnI CCSGG 4 cut(s) 76, 77, 110, 394
BcoDI GTCTC 2 cut(s) 209, 233
BfaI CTAG 2 cut(s) 327, 631
BfuAI ACCTGC 1 cut(s) 418
BisI GCNGC 4 cut(s) 26, 309, 318, 380
BlsI GCNGC 4 cut(s) 27, 310, 319, 381
Bme1390I CCNGG 4 cut(s) 76, 77, 110, 394
BmeT110I CYCGRG 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 283
BmrFI CCNGG 4 cut(s) 76, 77, 110, 394
BmsI GCATC 1 cut(s) 148
BpuMI CCSGG 4 cut(s) 76, 77, 110, 394
BsaJI CCNNGG 3 cut(s) 23, 75, 76
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse1I ACTGG 2 cut(s) 576, 598
Bse21I CCTNAGG 1 cut(s) 555
BseDI CCNNGG 3 cut(s) 23, 75, 76
BseGI GGATG 2 cut(s) 100, 163
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 36, 546
BseNI ACTGG 2 cut(s) 576, 598
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 3 cut(s) 320, 329, 391
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 4 cut(s) 76, 109, 365, 393
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 2 cut(s) 209, 233
BsmBI CGTCTC 1 cut(s) 209
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 75
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 2 cut(s) 229, 469
BspACI CCGC 5 cut(s) 23, 25, 161, 191, 311
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 2 cut(s) 37, 547
BspFNI CGCG 1 cut(s) 25
BspHI TCATGA 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 418
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 2 cut(s) 576, 598
BssECI CCNNGG 3 cut(s) 23, 75, 76
BssMI GATC 2 cut(s) 229, 469
Bst4CI ACNGT 2 cut(s) 54, 611
Bst6I CTCTTC 1 cut(s) 392
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 3 cut(s) 45, 555, 588
BstDSI CCRYGG 1 cut(s) 23
BstF5I GGATG 2 cut(s) 100, 163
BstFNI CGCG 1 cut(s) 25
BstKTI GATC 2 cut(s) 232, 472
BstMAI GTCTC 2 cut(s) 209, 233
BstMBI GATC 2 cut(s) 229, 469
BstMWI GCNNNNNNNGC 4 cut(s) 181, 190, 314, 317
BstSCI CCNGG 4 cut(s) 74, 75, 108, 392
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 3 cut(s) 320, 329, 391
Bsu36I CCTNAGG 1 cut(s) 555
BsuRI GGCC 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 23
BtsCI GGATG 2 cut(s) 100, 163
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 99, 583, 616
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 1 cut(s) 23
CciI TCATGA 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 283
Cfr42I CCGCGG 1 cut(s) 26
Cfr9I CCCGGG 1 cut(s) 75
Csp6I GTAC 1 cut(s) 335
CviAII CATG 1 cut(s) 31
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 3 cut(s) 45, 555, 588
DpnI GATC 2 cut(s) 231, 471
DpnII GATC 2 cut(s) 229, 469
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 27
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 1 cut(s) 34
FaiI YATR 5 cut(s) 32, 258, 428, 516, 607
FaqI GGGAC 1 cut(s) 125
FatI CATG 1 cut(s) 30
FauI CCCGC 1 cut(s) 30
FbaI TGATCA 2 cut(s) 229, 469
Fnu4HI GCNGC 4 cut(s) 26, 309, 318, 380
FokI GGATG 2 cut(s) 107, 170
Fsp4HI GCNGC 4 cut(s) 26, 309, 318, 380
FspBI CTAG 2 cut(s) 327, 631
GluI GCNGC 4 cut(s) 26, 309, 318, 380
HaeIII GGCC 1 cut(s) 284
HapII CCGG 4 cut(s) 76, 109, 365, 393
Hin1II CATG 1 cut(s) 34
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 2 cut(s) 87, 403
HpaII CCGG 4 cut(s) 76, 109, 365, 393
HphI GGTGA 1 cut(s) 92
Hpy166II GTNNAC 3 cut(s) 83, 345, 544
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 3 cut(s) 31, 127, 227
Hpy8I GTNNAC 3 cut(s) 83, 345, 544
HpyAV CCTTC 3 cut(s) 148, 244, 366
HpyCH4III ACNGT 2 cut(s) 54, 611
HpyCH4V TGCA 1 cut(s) 272
HpyF10VI GCNNNNNNNGC 4 cut(s) 181, 190, 314, 317
HpyF3I CTNAG 3 cut(s) 45, 555, 588
Hsp92II CATG 1 cut(s) 34
Ksp22I TGATCA 2 cut(s) 229, 469
KspI CCGCGG 1 cut(s) 26
Kzo9I GATC 2 cut(s) 229, 469
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 3 cut(s) 320, 329, 391
LweI GCATC 1 cut(s) 148
MaeI CTAG 2 cut(s) 327, 631
MalI GATC 2 cut(s) 231, 471
MboI GATC 2 cut(s) 229, 469
MboII GAAGA 4 cut(s) 20, 409, 466, 497
MluCI AATT 2 cut(s) 34, 465
MlyI GAGTC 1 cut(s) 96
MnlI CCTC 7 cut(s) 40, 85, 271, 274, 393, 532, 550
MseI TTAA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 25, 311
MspI CCGG 4 cut(s) 76, 109, 365, 393
MspR9I CCNGG 4 cut(s) 76, 77, 110, 394
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 4 cut(s) 181, 190, 314, 317
NciI CCSGG 4 cut(s) 76, 77, 110, 394
NdeII GATC 2 cut(s) 229, 469
NlaIII CATG 1 cut(s) 34
PagI TCATGA 1 cut(s) 30
PaqCI CACCTGC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 403
PkrI GCNGC 4 cut(s) 27, 310, 319, 381
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PspPI GGNCC 1 cut(s) 283
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
SacII CCGCGG 1 cut(s) 26
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 4 cut(s) 26, 309, 318, 380
Sau3AI GATC 2 cut(s) 229, 469
Sau96I GGNCC 1 cut(s) 283
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 4 cut(s) 76, 77, 110, 394
SetI ASST 9 cut(s) 202, 270, 358, 412, 427, 463, 493, 543, 556
SfaNI GCATC 1 cut(s) 148
Sfr303I CCGCGG 1 cut(s) 26
SgrBI CCGCGG 1 cut(s) 26
SmaI CCCGGG 1 cut(s) 77
Sse9I AATT 2 cut(s) 34, 465
SsiI CCGC 5 cut(s) 23, 25, 161, 191, 311
SspMI CTAG 2 cut(s) 327, 631
StyD4I CCNGG 4 cut(s) 74, 75, 108, 392
TaaI ACNGT 2 cut(s) 54, 611
TaqI TCGA 2 cut(s) 90, 126
TasI AATT 2 cut(s) 34, 465
TatI WGTACW 1 cut(s) 334
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 1 cut(s) 403
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 3 cut(s) 106, 583, 616
TseI GCWGC 3 cut(s) 308, 317, 379
TspDTI ATGAA 4 cut(s) 47, 75, 273, 594
TspGWI ACGGA 2 cut(s) 233, 362
TspMI CCCGGG 1 cut(s) 75
TspRI CASTG 3 cut(s) 106, 583, 616
XapI RAATTY 1 cut(s) 34
XmaI CCCGGG 1 cut(s) 75
XspI CTAG 2 cut(s) 327, 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.