FvH4_1g26260

N-terminal domain of NEFA-interacting nuclear protein NIP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005793
Physical Location & Seq
Forward (+)
18088073 .. 18092979
4907 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g26260.t2

Sequence Viewer

Length: 636 bp
ATGGGTGATGATTTCACACGCCCCCGCCTTATGAATTTCGTTTCTGAGGAACAGTTGGATCAAGCCAAGAAAACCCGGGGCGAACGAGTCGAGGATGGCACCGCCTACCGCGACAGACCCCTTTTTGAGATTCTCAAGGAAAATAAGGACAAGAAGGATGCCGAGTTCAACGAACGTTTCAAGCACAGACCTCCCAAAGCTCTGGATGATGACGAGACGGAGTTTCTTGATCAGTGGGAGACTTCGAAGAAGGAATATGAACGACAGATTGTGGATGAGGAAGCACAACAACTTTTGAGCTTTCAGGCAGCGGTGGCAGCAAAGTCGAGCATTGTACAAGAGTTAACGGAAATACCTTCTGTTCCGGTAGTCAAGGAGCAGCTGAAACCAACCGGGAAGAGGAATCCACCTGCTTTTCCTTTAGGCATGATTGTTAAAGTGAAGCCACAAGCGAAGAAAGCTAAAACTGATCAAGGGGGCATTGGAGAAGCTACAAGCACAGCGAAACCCACTAATGTCAACACAGATAAAACTTTAGAGGTAACGAAGACACCTGAGGGTGATGCTGATAATTCTAGTGCTGTTTCTGAGACTGGTCTTGTTTCATACAGTGATGACAGTGAAGATGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nefa_Nip30_N

Protein Analysis

212

Amino Acids

23.63

Weight (kDa)

4.81

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM192A_Fyv6_N PF10187 17 - 101 2.4e-16 FAM192A/Fyv6, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 418
Acc36I ACCTGC 1 cut(s) 418
AccB1I GGYRCC 1 cut(s) 98
AccII CGCG 1 cut(s) 111
AciI CCGC 4 cut(s) 25, 102, 109, 311
AclI AACGTT 1 cut(s) 175
AclWI GGATC 1 cut(s) 66
AcsI RAATTY 1 cut(s) 34
AfaI GTAC 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 399
AgsI TTSAA 2 cut(s) 169, 181
AloI GAACNNNNNNTCC 2 cut(s) 149, 181
AluBI AGCT 5 cut(s) 200, 300, 382, 461, 491
AluI AGCT 5 cut(s) 200, 300, 382, 461, 491
Alw26I GTCTC 3 cut(s) 209, 233, 584
AlwI GGATC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 75
ApeKI GCWGC 3 cut(s) 308, 317, 379
ApoI RAATTY 1 cut(s) 34
AsuC2I CCSGG 3 cut(s) 76, 77, 394
AsuHPI GGTGA 2 cut(s) 17, 572
AsuII TTCGAA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 75
AxyI CCTNAGG 1 cut(s) 555
BanI GGYRCC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 554
BbvI GCAGC 3 cut(s) 320, 329, 391
BccI CCATC 1 cut(s) 89
BcgI CGANNNNNNTGC 2 cut(s) 306, 340
BclI TGATCA 2 cut(s) 229, 469
BcnI CCSGG 3 cut(s) 76, 77, 394
BcoDI GTCTC 3 cut(s) 209, 233, 584
BfaI CTAG 1 cut(s) 576
BfuAI ACCTGC 1 cut(s) 418
BisI GCNGC 3 cut(s) 309, 318, 380
BlsI GCNGC 3 cut(s) 310, 319, 381
Bme1390I CCNGG 3 cut(s) 76, 77, 394
BmeT110I CYCGRG 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 3 cut(s) 76, 77, 394
BmsI GCATC 2 cut(s) 148, 553
BpiI GAAGAC 1 cut(s) 554
Bpu14I TTCGAA 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 3 cut(s) 76, 77, 394
BsaJI CCNNGG 2 cut(s) 75, 76
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 598
Bse21I CCTNAGG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 75, 76
BseGI GGATG 4 cut(s) 100, 163, 211, 280
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMII CTCAG 3 cut(s) 36, 546, 579
BseNI ACTGG 1 cut(s) 598
BseXI GCAGC 3 cut(s) 320, 329, 391
Bsh1236I CGCG 1 cut(s) 111
BshNI GGYRCC 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 75
BsiSI CCGG 3 cut(s) 76, 365, 393
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 3 cut(s) 209, 233, 584
BsmBI CGTCTC 1 cut(s) 209
BsoBI CYCGRG 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 245
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 3 cut(s) 58, 229, 469
BspACI CCGC 4 cut(s) 25, 102, 109, 311
BspCNI CTCAG 3 cut(s) 37, 547, 580
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 1 cut(s) 100
BspMI ACCTGC 1 cut(s) 418
BspPI GGATC 1 cut(s) 66
BspT104I TTCGAA 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 1 cut(s) 598
BssECI CCNNGG 2 cut(s) 75, 76
BssMI GATC 3 cut(s) 58, 229, 469
Bst4CI ACNGT 3 cut(s) 54, 611, 620
Bst6I CTCTTC 1 cut(s) 392
BstAUI TGTACA 1 cut(s) 334
BstBI TTCGAA 1 cut(s) 245
BstDEI CTNAG 3 cut(s) 45, 555, 588
BstF5I GGATG 4 cut(s) 100, 163, 211, 280
BstFNI CGCG 1 cut(s) 111
BstKTI GATC 3 cut(s) 61, 232, 472
BstMAI GTCTC 3 cut(s) 209, 233, 584
BstMBI GATC 3 cut(s) 58, 229, 469
BstMWI GCNNNNNNNGC 3 cut(s) 314, 317, 458
BstSCI CCNGG 3 cut(s) 74, 75, 392
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 3 cut(s) 320, 329, 391
BstV2I GAAGAC 1 cut(s) 554
BstXI CCANNNNNNTGG 1 cut(s) 202
Bsu36I CCTNAGG 1 cut(s) 555
BtsCI GGATG 4 cut(s) 100, 163, 211, 280
BtsIMutI CAGTG 3 cut(s) 239, 616, 625
BveI ACCTGC 1 cut(s) 418
Cfr9I CCCGGG 1 cut(s) 75
Csp6I GTAC 1 cut(s) 335
CviAII CATG 1 cut(s) 427
CviJI RGCY 7 cut(s) 65, 200, 300, 382, 445, 461, 491
CviKI_1 RGCY 7 cut(s) 65, 200, 300, 382, 445, 461, 491
CviQI GTAC 1 cut(s) 335
DdeI CTNAG 3 cut(s) 45, 555, 588
DpnI GATC 3 cut(s) 60, 231, 471
DpnII GATC 3 cut(s) 58, 229, 469
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 1 cut(s) 430
FaiI YATR 4 cut(s) 32, 258, 428, 607
FatI CATG 1 cut(s) 426
FauI CCCGC 1 cut(s) 32
FbaI TGATCA 2 cut(s) 229, 469
Fnu4HI GCNGC 3 cut(s) 309, 318, 380
FokI GGATG 4 cut(s) 107, 170, 218, 287
Fsp4HI GCNGC 3 cut(s) 309, 318, 380
FspBI CTAG 1 cut(s) 576
GluI GCNGC 3 cut(s) 309, 318, 380
HapII CCGG 3 cut(s) 76, 365, 393
Hin1II CATG 1 cut(s) 430
HincII GTYRAC 2 cut(s) 345, 520
HindII GTYRAC 2 cut(s) 345, 520
HinfI GANTC 3 cut(s) 87, 130, 403
HpaI GTTAAC 1 cut(s) 345
HpaII CCGG 3 cut(s) 76, 365, 393
HphI GGTGA 2 cut(s) 17, 572
Hpy166II GTNNAC 2 cut(s) 345, 520
Hpy188I TCNGA 2 cut(s) 46, 589
Hpy188III TCNNGA 2 cut(s) 203, 227
Hpy8I GTNNAC 2 cut(s) 345, 520
HpyAV CCTTC 3 cut(s) 148, 244, 366
HpyCH4III ACNGT 3 cut(s) 54, 611, 620
HpyCH4IV ACGT 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 317, 458
HpyF3I CTNAG 3 cut(s) 45, 555, 588
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 1 cut(s) 430
Ksp22I TGATCA 2 cut(s) 229, 469
KspAI GTTAAC 1 cut(s) 345
Kzo9I GATC 3 cut(s) 58, 229, 469
LmnI GCTCC 1 cut(s) 376
LpnPI CCDG 8 cut(s) 89, 188, 290, 378, 406, 423, 567, 579
Lsp1109I GCAGC 3 cut(s) 320, 329, 391
LweI GCATC 2 cut(s) 148, 553
MaeI CTAG 1 cut(s) 576
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 1 cut(s) 541
MalI GATC 3 cut(s) 60, 231, 471
MboI GATC 3 cut(s) 58, 229, 469
MboII GAAGA 5 cut(s) 259, 409, 466, 559, 635
MluCI AATT 2 cut(s) 34, 571
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 7 cut(s) 40, 85, 201, 271, 393, 532, 550
MseI TTAA 2 cut(s) 344, 435
MspA1I CMGCKG 2 cut(s) 311, 382
MspI CCGG 3 cut(s) 76, 365, 393
MspR9I CCNGG 3 cut(s) 76, 77, 394
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 3 cut(s) 314, 317, 458
NciI CCSGG 3 cut(s) 76, 77, 394
NdeII GATC 3 cut(s) 58, 229, 469
NlaIII CATG 1 cut(s) 430
NlaIV GGNNCC 1 cut(s) 100
NmeAIII GCCGAG 1 cut(s) 187
NspV TTCGAA 1 cut(s) 245
PaqCI CACCTGC 1 cut(s) 418
PfeI GAWTC 2 cut(s) 130, 403
PkrI GCNGC 3 cut(s) 310, 319, 381
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
Psp1406I AACGTT 1 cut(s) 175
PspN4I GGNNCC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 382
RsaI GTAC 1 cut(s) 336
RsaNI GTAC 1 cut(s) 335
SaqAI TTAA 2 cut(s) 344, 435
SatI GCNGC 3 cut(s) 309, 318, 380
Sau3AI GATC 3 cut(s) 58, 229, 469
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 3 cut(s) 76, 77, 394
SfaNI GCATC 2 cut(s) 148, 553
SfuI TTCGAA 1 cut(s) 245
SmaI CCCGGG 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
Sse9I AATT 2 cut(s) 34, 571
SsiI CCGC 4 cut(s) 25, 102, 109, 311
SspMI CTAG 1 cut(s) 576
StyD4I CCNGG 3 cut(s) 74, 75, 392
TaaI ACNGT 3 cut(s) 54, 611, 620
TaiI ACGT 1 cut(s) 178
TaqI TCGA 3 cut(s) 90, 245, 326
TasI AATT 2 cut(s) 34, 571
TatI WGTACW 1 cut(s) 334
TfiI GAWTC 2 cut(s) 130, 403
Tru1I TTAA 2 cut(s) 344, 435
Tru9I TTAA 2 cut(s) 344, 435
TscAI CASTG 3 cut(s) 239, 616, 625
TseI GCWGC 3 cut(s) 308, 317, 379
TspDTI ATGAA 3 cut(s) 47, 273, 594
TspGWI ACGGA 2 cut(s) 233, 362
TspMI CCCGGG 1 cut(s) 75
TspRI CASTG 3 cut(s) 239, 616, 625
XapI RAATTY 1 cut(s) 34
XmaI CCCGGG 1 cut(s) 75
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.