Rw1G024840

N-terminal domain of NEFA-interacting nuclear protein NIP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005793
Physical Location & Seq
Reverse (-)
51221507 .. 51223787
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G024840.1

Sequence Viewer

Length: 597 bp
ATGATACCTTTTCTTTTGACTTGTAACATTTTTAATACCAAGTCTCCCGTTAAAAAAAAAAATTTGAAAAAGAAGAAAGAAAACCTTAGGTCAATACCTTTCCTGAAAAATAAAAAGCAACTAAAATTGGAAGAAGCCAAGAAAACAAGGGGTGAACGAGTGGATGATGGAACTGCCCACAGGGATAGACCACTTTACGAGGTTCTGAAAGAAAACAAGGAGAGGAAGGATGCAGAGTTCAACGAACGTTTCAAGCACAGACCACCTAAAGCTTTGGACGATGAGGAGACTGACTTTCTTGATAGAATGCAGAAGGAAGCCCTCGCAGCCAAGGCCAGCGTCATGCATGAGCTTAAGGAAGCACATACTGAAAAGAAGAAGAATCCTACTCCTGGTTTATTGGGTATGATTATCAAAGTGAAGGAGCCACAAGCAAAGAAACCTAAAATTGAAGACGCTAACAACACAGTGAAAACTCCTCATGTTGATAGTAAAGATCATAGTTTAGAGGTAGTGAAGACTCCCAATGATGATAATACTGATAATTTCAGTGTTGTTGCTAAGACTGCTCTTGTTTCATATAGTGATCGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nefa_Nip30_N

Protein Analysis

198

Amino Acids

22.85

Weight (kDa)

9.52

Isoelectric Point (pI)

25.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM192A_Fyv6_N PF10187 37 - 121 6.8e-13 FAM192A/Fyv6, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 247
AcsI RAATTY 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 392
AflII CTTAAG 1 cut(s) 353
AgsI TTSAA 4 cut(s) 67, 241, 253, 452
AjnI CCWGG 1 cut(s) 391
AloI GAACNNNNNNTCC 2 cut(s) 221, 253
AluBI AGCT 2 cut(s) 272, 352
AluI AGCT 2 cut(s) 272, 352
Alw26I GTCTC 2 cut(s) 48, 281
AoxI GGCC 1 cut(s) 333
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 1 cut(s) 61
AsuHPI GGTGA 1 cut(s) 164
AxyI CCTNAGG 1 cut(s) 86
BbsI GAAGAC 2 cut(s) 459, 524
BbvI GCAGC 1 cut(s) 338
BccI CCATC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 393
BcoDI GTCTC 2 cut(s) 48, 281
BfrI CTTAAG 1 cut(s) 353
BisI GCNGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 328
Bme1390I CCNGG 1 cut(s) 393
BmiI GGNNCC 1 cut(s) 426
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 1 cut(s) 220
BpiI GAAGAC 2 cut(s) 459, 524
Bsa29I ATCGAT 1 cut(s) 589
BsaJI CCNNGG 1 cut(s) 330
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse21I CCTNAGG 1 cut(s) 86
BseBI CCWGG 1 cut(s) 393
BseCI ATCGAT 1 cut(s) 589
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 2 cut(s) 169, 235
BseLI CCNNNNNNNGG 1 cut(s) 392
BseRI GAGGAG 2 cut(s) 299, 468
BseXI GCAGC 1 cut(s) 338
BshFI GGCC 1 cut(s) 335
BshVI ATCGAT 1 cut(s) 589
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 2 cut(s) 48, 281
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 1 cut(s) 335
Bsp143I GATC 2 cut(s) 496, 586
BspANI GGCC 1 cut(s) 335
BspDI ATCGAT 1 cut(s) 589
BspLI GGNNCC 1 cut(s) 426
BspTI CTTAAG 1 cut(s) 353
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 2 cut(s) 496, 586
BssT1I CCWWGG 1 cut(s) 330
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 469
BstAFI CTTAAG 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 2 cut(s) 86, 561
BstF5I GGATG 2 cut(s) 169, 235
BstKTI GATC 2 cut(s) 499, 589
BstMAI GTCTC 2 cut(s) 48, 281
BstMBI GATC 2 cut(s) 496, 586
BstMWI GCNNNNNNNGC 3 cut(s) 326, 332, 566
BstNI CCWGG 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 391
BstV1I GCAGC 1 cut(s) 338
BstV2I GAAGAC 2 cut(s) 459, 524
Bsu15I ATCGAT 1 cut(s) 589
Bsu36I CCTNAGG 1 cut(s) 86
BsuRI GGCC 1 cut(s) 335
BsuTUI ATCGAT 1 cut(s) 589
BtsCI GGATG 2 cut(s) 169, 235
BtsIMutI CAGTG 2 cut(s) 474, 556
Cac8I GCNNGC 1 cut(s) 337
ClaI ATCGAT 1 cut(s) 589
CseI GACGC 2 cut(s) 328, 464
CviAII CATG 3 cut(s) 343, 347, 482
CviJI RGCY 7 cut(s) 137, 272, 320, 329, 335, 352, 427
CviKI_1 RGCY 7 cut(s) 137, 272, 320, 329, 335, 352, 427
DdeI CTNAG 2 cut(s) 86, 561
DpnI GATC 2 cut(s) 498, 588
DpnII GATC 2 cut(s) 496, 586
Eco130I CCWWGG 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 330
EcoT22I ATGCAT 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 3 cut(s) 346, 350, 485
FaiI YATR 8 cut(s) 344, 348, 366, 407, 483, 501, 580, 582
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FatI CATG 3 cut(s) 342, 346, 481
Fnu4HI GCNGC 1 cut(s) 327
FokI GGATG 2 cut(s) 176, 242
Fsp4HI GCNGC 1 cut(s) 327
GluI GCNGC 1 cut(s) 327
HaeIII GGCC 1 cut(s) 335
HgaI GACGC 2 cut(s) 328, 464
Hin1II CATG 3 cut(s) 346, 350, 485
HindIII AAGCTT 1 cut(s) 270
HinfI GANTC 2 cut(s) 382, 520
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 2 cut(s) 103, 299
Hpy8I GTNNAC 1 cut(s) 155
HpyAV CCTTC 3 cut(s) 220, 307, 415
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 3 cut(s) 233, 310, 346
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 332, 566
HpyF3I CTNAG 2 cut(s) 86, 561
HpySE526I ACGT 1 cut(s) 247
Hsp92II CATG 3 cut(s) 346, 350, 485
Kzo9I GATC 2 cut(s) 496, 586
LmnI GCTCC 1 cut(s) 424
LpnPI CCDG 5 cut(s) 116, 166, 349, 378, 405
Lsp1109I GCAGC 1 cut(s) 338
LweI GCATC 1 cut(s) 220
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 1 cut(s) 23
MalI GATC 2 cut(s) 498, 588
MboI GATC 2 cut(s) 496, 586
MboII GAAGA 6 cut(s) 85, 143, 388, 391, 464, 529
MluCI AATT 4 cut(s) 61, 125, 447, 544
MlyI GAGTC 1 cut(s) 514
MnlI CCTC 6 cut(s) 193, 216, 277, 332, 489, 502
Mph1103I ATGCAT 1 cut(s) 348
MseI TTAA 3 cut(s) 33, 51, 354
MspCI CTTAAG 1 cut(s) 353
MspR9I CCNGG 1 cut(s) 393
Mva1269I GAATGC 1 cut(s) 312
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 3 cut(s) 326, 332, 566
NdeII GATC 2 cut(s) 496, 586
NlaIII CATG 3 cut(s) 346, 350, 485
NlaIV GGNNCC 1 cut(s) 426
NsiI ATGCAT 1 cut(s) 348
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 382
PkrI GCNGC 1 cut(s) 328
PleI GAGTC 1 cut(s) 514
PpsI GAGTC 1 cut(s) 514
Psp1406I AACGTT 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 1 cut(s) 426
SaqAI TTAA 3 cut(s) 33, 51, 354
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 2 cut(s) 496, 586
SchI GAGTC 1 cut(s) 514
ScrFI CCNGG 1 cut(s) 393
SfaNI GCATC 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 4 cut(s) 61, 125, 447, 544
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 1 cut(s) 250
TaqI TCGA 1 cut(s) 589
TasI AATT 4 cut(s) 61, 125, 447, 544
TfiI GAWTC 1 cut(s) 382
Tru1I TTAA 3 cut(s) 33, 51, 354
Tru9I TTAA 3 cut(s) 33, 51, 354
TscAI CASTG 2 cut(s) 474, 556
TseI GCWGC 1 cut(s) 326
TspDTI ATGAA 1 cut(s) 567
TspRI CASTG 2 cut(s) 474, 556
Vha464I CTTAAG 1 cut(s) 353
XapI RAATTY 1 cut(s) 61
Zsp2I ATGCAT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.