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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G62900 AT3G62900 AT3G62900 |
| fragaria_vesca | FvH4_2g25070 |
| malus_domestica | MD03G1282000.v1.1 MD11G1301800.v1.1 |
| prunus_persica | Prupe.8G255400_v2.0.a1 |
| pyrus_communis | pycom03g22260 pycom03g22270 pycom03g22280 pycom11g26440 |
| rosa_chinensis | RchiOBHm_Chr6g0293091 |
| rosa_laevigata | RLG00000011972 |
| rosa_multiflora | Rmu_sc0001042.1_g000002 |
| rosa_roxburghii | Rroxscaffold_7G00174420 |
| rosa_rugosa | Rorug06G0232700 |
| rosa_samantha | Rh6AG345500 Rh6BG353300 Rh6CG359700 Rh6DG345600 |
| rosa_wichuraiana | Rw6G030110 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 4146 |
| AatII | GACGTC | 1 cut(s) | 2287 |
| AbsI | CCTCGAGG | 1 cut(s) | 4045 |
| Acc16I | TGCGCA | 1 cut(s) | 3465 |
| Acc36I | ACCTGC | 1 cut(s) | 2774 |
| Acc65I | GGTACC | 2 cut(s) | 490, 3923 |
| AccB1I | GGYRCC | 2 cut(s) | 490, 3923 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 4159 |
| AccBSI | CCGCTC | 1 cut(s) | 3188 |
| AccI | GTMKAC | 3 cut(s) | 764, 1077, 3912 |
| AccII | CGCG | 5 cut(s) | 2603, 2661, 2934, 2989, 3767 |
| AclI | AACGTT | 2 cut(s) | 217, 1629 |
| AclWI | GGATC | 9 cut(s) | 690, 723, 1873, 2157, 2865, 2878, 2920, 2933, 3006 |
| AcoI | YGGCCR | 4 cut(s) | 2097, 2223, 2971, 3281 |
| AcsI | RAATTY | 4 cut(s) | 463, 2074, 2798, 3529 |
| AcuI | CTGAAG | 6 cut(s) | 436, 491, 1185, 1539, 1860, 2364 |
| AcvI | CACGTG | 1 cut(s) | 4058 |
| AcyI | GRCGYC | 2 cut(s) | 1758, 2284 |
| AdeI | CACNNNGTG | 1 cut(s) | 1460 |
| AfaI | GTAC | 2 cut(s) | 492, 3925 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 3293, 4159, 4206 |
| AflII | CTTAAG | 1 cut(s) | 3578 |
| AflIII | ACRYGT | 1 cut(s) | 4057 |
| AgeI | ACCGGT | 1 cut(s) | 4206 |
| AjiI | CACGTC | 2 cut(s) | 2316, 3967 |
| AjnI | CCWGG | 7 cut(s) | 776, 1813, 1896, 1944, 3057, 3741, 3850 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1129, 1161 |
| Alw21I | GWGCWC | 4 cut(s) | 1796, 2020, 3552, 3561 |
| Alw26I | GTCTC | 9 cut(s) | 237, 645, 890, 1397, 1497, 2350, 3136, 3196, 3527 |
| Alw44I | GTGCAC | 1 cut(s) | 1792 |
| AlwI | GGATC | 9 cut(s) | 690, 723, 1873, 2157, 2865, 2878, 2920, 2933, 3006 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2387 |
| Ama87I | CYCGRG | 4 cut(s) | 2293, 2340, 2490, 4045 |
| ApaLI | GTGCAC | 1 cut(s) | 1792 |
| ApoI | RAATTY | 4 cut(s) | 463, 2074, 2798, 3529 |
| AsiGI | ACCGGT | 1 cut(s) | 4206 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 1773, 2247, 2563, 2798, 3079 |
| Asp718I | GGTACC | 2 cut(s) | 490, 3923 |
| AspLEI | GCGC | 5 cut(s) | 1123, 2934, 2936, 3466, 4136 |
| AspS9I | GGNCC | 7 cut(s) | 919, 1634, 1925, 3093, 3137, 3649, 4096 |
| AsuC2I | CCSGG | 1 cut(s) | 2720 |
| AsuII | TTCGAA | 1 cut(s) | 2567 |
| AvaI | CYCGRG | 4 cut(s) | 2293, 2340, 2490, 4045 |
| AvaII | GGWCC | 4 cut(s) | 1634, 3093, 3137, 3649 |
| AxyI | CCTNAGG | 1 cut(s) | 618 |
| BaeGI | GKGCMC | 2 cut(s) | 1796, 3703 |
| BalI | TGGCCA | 2 cut(s) | 2225, 2973 |
| BamHI | GGATCC | 1 cut(s) | 2870 |
| BanI | GGYRCC | 2 cut(s) | 490, 3923 |
| BanII | GRGCYC | 1 cut(s) | 3561 |
| BbrPI | CACGTG | 1 cut(s) | 4058 |
| BbsI | GAAGAC | 7 cut(s) | 248, 442, 1353, 1753, 1776, 2685, 2874 |
| Bbv12I | GWGCWC | 4 cut(s) | 1796, 2020, 3552, 3561 |
| BceAI | ACGGC | 2 cut(s) | 1430, 3296 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 500, 534, 3745, 3779 |
| BciT130I | CCWGG | 7 cut(s) | 778, 1815, 1898, 1946, 3059, 3743, 3852 |
| BciVI | GTATCC | 1 cut(s) | 1199 |
| BclI | TGATCA | 1 cut(s) | 1695 |
| BcnI | CCSGG | 1 cut(s) | 2720 |
| BcoDI | GTCTC | 9 cut(s) | 237, 645, 890, 1397, 1497, 2350, 3136, 3196, 3527 |
| BfmI | CTRYAG | 2 cut(s) | 267, 2025 |
| BfoI | RGCGCY | 1 cut(s) | 4137 |
| BfrI | CTTAAG | 1 cut(s) | 3578 |
| BfuAI | ACCTGC | 1 cut(s) | 2774 |
| BfuI | GTATCC | 1 cut(s) | 1199 |
| BglII | AGATCT | 3 cut(s) | 278, 1111, 2203 |
| Bme1390I | CCNGG | 8 cut(s) | 778, 1815, 1898, 1946, 2720, 3059, 3743, 3852 |
| Bme18I | GGWCC | 4 cut(s) | 1634, 3093, 3137, 3649 |
| BmeT110I | CYCGRG | 4 cut(s) | 2293, 2340, 2490, 4045 |
| BmgBI | CACGTC | 2 cut(s) | 2316, 3967 |
| BmgT120I | GGNCC | 7 cut(s) | 919, 1634, 1925, 3093, 3137, 3649, 4096 |
| BmiI | GGNNCC | 9 cut(s) | 492, 615, 822, 1353, 2514, 2872, 3846, 3925, 3962 |
| BmrFI | CCNGG | 8 cut(s) | 778, 1815, 1898, 1946, 2720, 3059, 3743, 3852 |
| BmrI | ACTGGG | 2 cut(s) | 1914, 2415 |
| BmuI | ACTGGG | 2 cut(s) | 1914, 2415 |
| BoxI | GACNNNNGTC | 1 cut(s) | 902 |
| BpiI | GAAGAC | 7 cut(s) | 248, 442, 1353, 1753, 1776, 2685, 2874 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 20, 52 |
| BpmI | CTGGAG | 2 cut(s) | 855, 1836 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1929, 4017 |
| Bpu14I | TTCGAA | 1 cut(s) | 2567 |
| BpuEI | CTTGAG | 4 cut(s) | 2596, 3509, 3566, 3913 |
| BpuMI | CCSGG | 1 cut(s) | 2720 |
| BsaAI | YACGTR | 1 cut(s) | 4058 |
| BsaBI | GATNNNNATC | 2 cut(s) | 84, 3036 |
| BsaHI | GRCGYC | 2 cut(s) | 1758, 2284 |
| BsaI | GGTCTC | 2 cut(s) | 237, 3196 |
| BsaWI | WCCGGW | 1 cut(s) | 4206 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 265, 295, 625, 655, 1343, 1373, 3752, 3782 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 3293, 4159, 4206 |
| Bse118I | RCCGGY | 1 cut(s) | 4206 |
| Bse1I | ACTGG | 8 cut(s) | 411, 648, 1005, 1920, 2392, 2410, 3000, 3798 |
| Bse21I | CCTNAGG | 1 cut(s) | 618 |
| Bse3DI | GCAATG | 5 cut(s) | 322, 1837, 1875, 2539, 3055 |
| Bse8I | GATNNNNATC | 2 cut(s) | 84, 3036 |
| BseBI | CCWGG | 7 cut(s) | 778, 1815, 1898, 1946, 3059, 3743, 3852 |
| BseJI | GATNNNNATC | 2 cut(s) | 84, 3036 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 3293, 4159, 4206 |
| BseMI | GCAATG | 5 cut(s) | 322, 1837, 1875, 2539, 3055 |
| BseNI | ACTGG | 8 cut(s) | 411, 648, 1005, 1920, 2392, 2410, 3000, 3798 |
| BsePI | GCGCGC | 1 cut(s) | 2932 |
| BseRI | GAGGAG | 7 cut(s) | 20, 626, 693, 892, 2338, 2388, 3262 |
| BseSI | GKGCMC | 2 cut(s) | 1796, 3703 |
| BseYI | CCCAGC | 1 cut(s) | 3956 |
| BsgI | GTGCAG | 1 cut(s) | 3960 |
| Bsh1236I | CGCG | 5 cut(s) | 2603, 2661, 2934, 2989, 3767 |
| BshNI | GGYRCC | 2 cut(s) | 490, 3923 |
| BshTI | ACCGGT | 1 cut(s) | 4206 |
| BsiHKAI | GWGCWC | 4 cut(s) | 1796, 2020, 3552, 3561 |
| BsiHKCI | CYCGRG | 4 cut(s) | 2293, 2340, 2490, 4045 |
| BsiSI | CCGG | 2 cut(s) | 2720, 4207 |
| BslFI | GGGAC | 7 cut(s) | 286, 289, 1526, 1581, 1871, 2097, 2291 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 3293, 4159, 4206 |
| BsmAI | GTCTC | 9 cut(s) | 237, 645, 890, 1397, 1497, 2350, 3136, 3196, 3527 |
| BsmBI | CGTCTC | 1 cut(s) | 3527 |
| BsmFI | GGGAC | 7 cut(s) | 286, 289, 1526, 1581, 1871, 2097, 2291 |
| BsmI | GAATGC | 1 cut(s) | 523 |
| Bso31I | GGTCTC | 2 cut(s) | 237, 3196 |
| BsoBI | CYCGRG | 4 cut(s) | 2293, 2340, 2490, 4045 |
| Bsp119I | TTCGAA | 1 cut(s) | 2567 |
| Bsp1286I | GDGCHC | 5 cut(s) | 1796, 2020, 3552, 3561, 3703 |
| Bsp19I | CCATGG | 1 cut(s) | 2440 |
| BspFNI | CGCG | 5 cut(s) | 2603, 2661, 2934, 2989, 3767 |
| BspHI | TCATGA | 2 cut(s) | 1126, 2473 |
| BspLI | GGNNCC | 9 cut(s) | 492, 615, 822, 1353, 2514, 2872, 3846, 3925, 3962 |
| BspMAI | CTGCAG | 1 cut(s) | 2029 |
| BspMI | ACCTGC | 1 cut(s) | 2774 |
| BspPI | GGATC | 9 cut(s) | 690, 723, 1873, 2157, 2865, 2878, 2920, 2933, 3006 |
| BspQI | GCTCTTC | 1 cut(s) | 2165 |
| BspT104I | TTCGAA | 1 cut(s) | 2567 |
| BspT107I | GGYRCC | 2 cut(s) | 490, 3923 |
| BspTI | CTTAAG | 1 cut(s) | 3578 |
| BspTNI | GGTCTC | 2 cut(s) | 237, 3196 |
| BsrBI | CCGCTC | 1 cut(s) | 3188 |
| BsrDI | GCAATG | 5 cut(s) | 322, 1837, 1875, 2539, 3055 |
| BsrFI | RCCGGY | 1 cut(s) | 4206 |
| BsrI | ACTGG | 8 cut(s) | 411, 648, 1005, 1920, 2392, 2410, 3000, 3798 |
| BssAI | RCCGGY | 1 cut(s) | 4206 |
| BssHII | GCGCGC | 1 cut(s) | 2932 |
| BssNAI | GTATAC | 1 cut(s) | 765 |
| BssNI | GRCGYC | 2 cut(s) | 1758, 2284 |
| BssT1I | CCWWGG | 7 cut(s) | 121, 541, 948, 1029, 1211, 2440, 3301 |
| Bst1107I | GTATAC | 1 cut(s) | 765 |
| Bst2UI | CCWGG | 7 cut(s) | 778, 1815, 1898, 1946, 3059, 3743, 3852 |
| Bst6I | CTCTTC | 4 cut(s) | 1521, 1680, 2165, 2499 |
| BstACI | GRCGYC | 2 cut(s) | 1758, 2284 |
| BstAFI | CTTAAG | 1 cut(s) | 3578 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1865 |
| BstBAI | YACGTR | 1 cut(s) | 4058 |
| BstBI | TTCGAA | 1 cut(s) | 2567 |
| BstC8I | GCNNGC | 8 cut(s) | 34, 1607, 2223, 2934, 3425, 3468, 3574, 4188 |
| BstDSI | CCRYGG | 2 cut(s) | 2440, 2987 |
| BstFNI | CGCG | 5 cut(s) | 2603, 2661, 2934, 2989, 3767 |
| BstH2I | RGCGCY | 1 cut(s) | 4137 |
| BstHHI | GCGC | 5 cut(s) | 1123, 2934, 2936, 3466, 4136 |
| BstMAI | GTCTC | 9 cut(s) | 237, 645, 890, 1397, 1497, 2350, 3136, 3196, 3527 |
| BstNI | CCWGG | 7 cut(s) | 778, 1815, 1898, 1946, 3059, 3743, 3852 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 902 |
| BstSCI | CCNGG | 8 cut(s) | 776, 1813, 1896, 1944, 2718, 3057, 3741, 3850 |
| BstSFI | CTRYAG | 2 cut(s) | 267, 2025 |
| BstSLI | GKGCMC | 2 cut(s) | 1796, 3703 |
| BstUI | CGCG | 5 cut(s) | 2603, 2661, 2934, 2989, 3767 |
| BstV2I | GAAGAC | 7 cut(s) | 248, 442, 1353, 1753, 1776, 2685, 2874 |
| BstX2I | RGATCY | 7 cut(s) | 278, 715, 1111, 1878, 2203, 2870, 2912 |
| BstYI | RGATCY | 7 cut(s) | 278, 715, 1111, 1878, 2203, 2870, 2912 |
| BstZ17I | GTATAC | 1 cut(s) | 765 |
| Bsu36I | CCTNAGG | 1 cut(s) | 618 |
| BsuI | GTATCC | 1 cut(s) | 1199 |
| BtgI | CCRYGG | 2 cut(s) | 2440, 2987 |
| BtrI | CACGTC | 2 cut(s) | 2316, 3967 |
| BtsI | GCAGTG | 3 cut(s) | 786, 2151, 3675 |
| BveI | ACCTGC | 1 cut(s) | 2774 |
| Cac8I | GCNNGC | 8 cut(s) | 34, 1607, 2223, 2934, 3425, 3468, 3574, 4188 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2387 |
| CciI | TCATGA | 2 cut(s) | 1126, 2473 |
| CfoI | GCGC | 5 cut(s) | 1123, 2934, 2936, 3466, 4136 |
| Cfr10I | RCCGGY | 1 cut(s) | 4206 |
| Cfr13I | GGNCC | 7 cut(s) | 919, 1634, 1925, 3093, 3137, 3649, 4096 |
| Cfr42I | CCGCGG | 1 cut(s) | 2990 |
| CseI | GACGC | 1 cut(s) | 1747 |
| Csp6I | GTAC | 2 cut(s) | 491, 3924 |
| CspAI | ACCGGT | 1 cut(s) | 4206 |
| CviAII | CATG | 9 cut(s) | 1127, 1645, 2441, 2474, 2867, 2876, 3704, 3757, 4159 |
| CviQI | GTAC | 2 cut(s) | 491, 3924 |
| DraI | TTTAAA | 1 cut(s) | 3486 |
| DraIII | CACNNNGTG | 1 cut(s) | 1460 |
| EaeI | YGGCCR | 4 cut(s) | 2097, 2223, 2971, 3281 |
| Eam1104I | CTCTTC | 4 cut(s) | 1521, 1680, 2165, 2499 |
| EarI | CTCTTC | 4 cut(s) | 1521, 1680, 2165, 2499 |
| EciI | GGCGGA | 2 cut(s) | 1563, 3837 |
| Ecl136II | GAGCTC | 1 cut(s) | 3559 |
| Eco130I | CCWWGG | 7 cut(s) | 121, 541, 948, 1029, 1211, 2440, 3301 |
| Eco24I | GRGCYC | 1 cut(s) | 3561 |
| Eco31I | GGTCTC | 2 cut(s) | 237, 3196 |
| Eco32I | GATATC | 1 cut(s) | 3812 |
| Eco47I | GGWCC | 4 cut(s) | 1634, 3093, 3137, 3649 |
| Eco53kI | GAGCTC | 1 cut(s) | 3559 |
| Eco57I | CTGAAG | 6 cut(s) | 436, 491, 1185, 1539, 1860, 2364 |
| Eco72I | CACGTG | 1 cut(s) | 4058 |
| Eco81I | CCTNAGG | 1 cut(s) | 618 |
| Eco88I | CYCGRG | 4 cut(s) | 2293, 2340, 2490, 4045 |
| EcoICRI | GAGCTC | 1 cut(s) | 3559 |
| EcoO109I | RGGNCCY | 1 cut(s) | 919 |
| EcoRI | GAATTC | 2 cut(s) | 463, 3529 |
| EcoRII | CCWGG | 7 cut(s) | 776, 1813, 1896, 1944, 3057, 3741, 3850 |
| EcoRV | GATATC | 1 cut(s) | 3812 |
| EcoT14I | CCWWGG | 7 cut(s) | 121, 541, 948, 1029, 1211, 2440, 3301 |
| EcoT22I | ATGCAT | 2 cut(s) | 1027, 3758 |
| EcoT38I | GRGCYC | 1 cut(s) | 3561 |
| ErhI | CCWWGG | 7 cut(s) | 121, 541, 948, 1029, 1211, 2440, 3301 |
| Esp3I | CGTCTC | 1 cut(s) | 3527 |
| FaeI | CATG | 9 cut(s) | 1130, 1648, 2444, 2477, 2870, 2879, 3707, 3760, 4162 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 3412, 3444, 3561, 3593 |
| FaqI | GGGAC | 7 cut(s) | 286, 289, 1526, 1581, 1871, 2097, 2291 |
| FatI | CATG | 9 cut(s) | 1126, 1644, 2440, 2473, 2866, 2875, 3703, 3756, 4158 |
| FauI | CCCGC | 1 cut(s) | 2666 |
| FbaI | TGATCA | 1 cut(s) | 1695 |
| FblI | GTMKAC | 3 cut(s) | 764, 1077, 3912 |
| FriOI | GRGCYC | 1 cut(s) | 3561 |
| FspI | TGCGCA | 1 cut(s) | 3465 |
| GlaI | GCGC | 5 cut(s) | 1122, 2933, 2935, 3465, 4135 |
| GsaI | CCCAGC | 1 cut(s) | 3960 |
| GsuI | CTGGAG | 2 cut(s) | 855, 1836 |
| HaeII | RGCGCY | 1 cut(s) | 4137 |
| HapII | CCGG | 2 cut(s) | 2720, 4207 |
| HgaI | GACGC | 1 cut(s) | 1747 |
| HhaI | GCGC | 5 cut(s) | 1123, 2934, 2936, 3466, 4136 |
| Hin1I | GRCGYC | 2 cut(s) | 1758, 2284 |
| Hin1II | CATG | 9 cut(s) | 1130, 1648, 2444, 2477, 2870, 2879, 3707, 3760, 4162 |
| Hin6I | GCGC | 5 cut(s) | 1121, 2932, 2934, 3464, 4134 |
| HinP1I | GCGC | 5 cut(s) | 1121, 2932, 2934, 3464, 4134 |
| HincII | GTYRAC | 1 cut(s) | 1078 |
| HindII | GTYRAC | 1 cut(s) | 1078 |
| HindIII | AAGCTT | 5 cut(s) | 107, 473, 1242, 3570, 4184 |
| HpaII | CCGG | 2 cut(s) | 2720, 4207 |
| Hpy166II | GTNNAC | 7 cut(s) | 765, 1078, 1794, 1822, 1941, 3895, 3913 |
| Hpy8I | GTNNAC | 7 cut(s) | 765, 1078, 1794, 1822, 1941, 3895, 3913 |
| Hpy99I | CGWCG | 1 cut(s) | 3045 |
| Hsp92I | GRCGYC | 2 cut(s) | 1758, 2284 |
| Hsp92II | CATG | 9 cut(s) | 1130, 1648, 2444, 2477, 2870, 2879, 3707, 3760, 4162 |
| HspAI | GCGC | 5 cut(s) | 1121, 2932, 2934, 3464, 4134 |
| KpnI | GGTACC | 2 cut(s) | 494, 3927 |
| Ksp22I | TGATCA | 1 cut(s) | 1695 |
| KspI | CCGCGG | 1 cut(s) | 2990 |
| LguI | GCTCTTC | 1 cut(s) | 2165 |
| LmnI | GCTCC | 8 cut(s) | 613, 879, 1303, 1351, 2375, 2512, 2660, 3731 |
| MaeIII | GTNAC | 3 cut(s) | 555, 1721, 3388 |
| MbiI | CCGCTC | 1 cut(s) | 3188 |
| MflI | RGATCY | 7 cut(s) | 278, 715, 1111, 1878, 2203, 2870, 2912 |
| MhlI | GDGCHC | 5 cut(s) | 1796, 2020, 3552, 3561, 3703 |
| MlsI | TGGCCA | 2 cut(s) | 2225, 2973 |
| MluNI | TGGCCA | 2 cut(s) | 2225, 2973 |
| MmeI | TCCRAC | 3 cut(s) | 656, 2523, 2739 |
| Mox20I | TGGCCA | 2 cut(s) | 2225, 2973 |
| Mph1103I | ATGCAT | 2 cut(s) | 1027, 3758 |
| MroXI | GAANNNNTTC | 5 cut(s) | 1773, 2247, 2563, 2798, 3079 |
| MscI | TGGCCA | 2 cut(s) | 2225, 2973 |
| MseI | TTAA | 8 cut(s) | 657, 1803, 2415, 3485, 3579, 3783, 4067, 4128 |
| MslI | CAYNNNNRTG | 1 cut(s) | 3708 |
| Msp20I | TGGCCA | 2 cut(s) | 2225, 2973 |
| MspA1I | CMGCKG | 4 cut(s) | 161, 2989, 3359, 3515 |
| MspCI | CTTAAG | 1 cut(s) | 3578 |
| MspI | CCGG | 2 cut(s) | 2720, 4207 |
| MspR9I | CCNGG | 8 cut(s) | 778, 1815, 1898, 1946, 2720, 3059, 3743, 3852 |
| Mva1269I | GAATGC | 1 cut(s) | 523 |
| MvaI | CCWGG | 7 cut(s) | 778, 1815, 1898, 1946, 3059, 3743, 3852 |
| MvnI | CGCG | 5 cut(s) | 2603, 2661, 2934, 2989, 3767 |
| NciI | CCSGG | 1 cut(s) | 2720 |
| NcoI | CCATGG | 1 cut(s) | 2440 |
| NlaIII | CATG | 9 cut(s) | 1130, 1648, 2444, 2477, 2870, 2879, 3707, 3760, 4162 |
| NlaIV | GGNNCC | 9 cut(s) | 492, 615, 822, 1353, 2514, 2872, 3846, 3925, 3962 |
| NmeAIII | GCCGAG | 2 cut(s) | 1501, 2125 |
| NmuCI | GTSAC | 3 cut(s) | 555, 1721, 3388 |
| NsbI | TGCGCA | 1 cut(s) | 3465 |
| NsiI | ATGCAT | 2 cut(s) | 1027, 3758 |
| NspV | TTCGAA | 1 cut(s) | 2567 |
| PaeR7I | CTCGAG | 3 cut(s) | 2293, 2490, 4045 |
| PagI | TCATGA | 2 cut(s) | 1126, 2473 |
| PasI | CCCWGGG | 1 cut(s) | 1945 |
| PauI | GCGCGC | 1 cut(s) | 2932 |
| PciSI | GCTCTTC | 1 cut(s) | 2165 |
| PctI | GAATGC | 1 cut(s) | 523 |
| PdmI | GAANNNNTTC | 5 cut(s) | 1773, 2247, 2563, 2798, 3079 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 4159 |
| PinAI | ACCGGT | 1 cut(s) | 4206 |
| PmaCI | CACGTG | 1 cut(s) | 4058 |
| PmlI | CACGTG | 1 cut(s) | 4058 |
| Ppu21I | YACGTR | 1 cut(s) | 4058 |
| PshAI | GACNNNNGTC | 1 cut(s) | 902 |
| PsiI | TTATAA | 1 cut(s) | 4146 |
| Psp124BI | GAGCTC | 1 cut(s) | 3561 |
| Psp1406I | AACGTT | 2 cut(s) | 217, 1629 |
| Psp6I | CCWGG | 7 cut(s) | 776, 1813, 1896, 1944, 3057, 3741, 3850 |
| PspCI | CACGTG | 1 cut(s) | 4058 |
| PspFI | CCCAGC | 1 cut(s) | 3956 |
| PspGI | CCWGG | 7 cut(s) | 776, 1813, 1896, 1944, 3057, 3741, 3850 |
| PspN4I | GGNNCC | 9 cut(s) | 492, 615, 822, 1353, 2514, 2872, 3846, 3925, 3962 |
| PspPI | GGNCC | 7 cut(s) | 919, 1634, 1925, 3093, 3137, 3649, 4096 |
| PspXI | VCTCGAGB | 3 cut(s) | 2293, 2490, 4045 |
| PstI | CTGCAG | 1 cut(s) | 2029 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2387 |
| PsuI | RGATCY | 7 cut(s) | 278, 715, 1111, 1878, 2203, 2870, 2912 |
| PteI | GCGCGC | 1 cut(s) | 2932 |
| PvuII | CAGCTG | 3 cut(s) | 161, 3359, 3515 |
| RsaI | GTAC | 2 cut(s) | 492, 3925 |
| RsaNI | GTAC | 2 cut(s) | 491, 3924 |
| RseI | CAYNNNNRTG | 1 cut(s) | 3708 |
| SacI | GAGCTC | 1 cut(s) | 3561 |
| SacII | CCGCGG | 1 cut(s) | 2990 |
| SalI | GTCGAC | 1 cut(s) | 1076 |
| SapI | GCTCTTC | 1 cut(s) | 2165 |
| SaqAI | TTAA | 8 cut(s) | 657, 1803, 2415, 3485, 3579, 3783, 4067, 4128 |
| Sau96I | GGNCC | 7 cut(s) | 919, 1634, 1925, 3093, 3137, 3649, 4096 |
| ScrFI | CCNGG | 8 cut(s) | 778, 1815, 1898, 1946, 2720, 3059, 3743, 3852 |
| SduI | GDGCHC | 5 cut(s) | 1796, 2020, 3552, 3561, 3703 |
| SfcI | CTRYAG | 2 cut(s) | 267, 2025 |
| Sfr274I | CTCGAG | 3 cut(s) | 2293, 2490, 4045 |
| Sfr303I | CCGCGG | 1 cut(s) | 2990 |
| SfuI | TTCGAA | 1 cut(s) | 2567 |
| SgrBI | CCGCGG | 1 cut(s) | 2990 |
| SinI | GGWCC | 4 cut(s) | 1634, 3093, 3137, 3649 |
| SlaI | CTCGAG | 3 cut(s) | 2293, 2490, 4045 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 3708 |
| SmlI | CTYRAG | 8 cut(s) | 2293, 2490, 2611, 3524, 3545, 3578, 3928, 4045 |
| SmoI | CTYRAG | 8 cut(s) | 2293, 2490, 2611, 3524, 3545, 3578, 3928, 4045 |
| SspI | AATATT | 1 cut(s) | 46 |
| SstI | GAGCTC | 1 cut(s) | 3561 |
| StyD4I | CCNGG | 8 cut(s) | 776, 1813, 1896, 1944, 2718, 3057, 3741, 3850 |
| StyI | CCWWGG | 7 cut(s) | 121, 541, 948, 1029, 1211, 2440, 3301 |
| TaqII | GACCGA | 1 cut(s) | 3081 |
| TauI | GCSGC | 4 cut(s) | 1612, 1744, 2606, 3473 |
| Tru1I | TTAA | 8 cut(s) | 657, 1803, 2415, 3485, 3579, 3783, 4067, 4128 |
| Tru9I | TTAA | 8 cut(s) | 657, 1803, 2415, 3485, 3579, 3783, 4067, 4128 |
| TseFI | GTSAC | 3 cut(s) | 555, 1721, 3388 |
| Tsp45I | GTSAC | 3 cut(s) | 555, 1721, 3388 |
| TspGWI | ACGGA | 2 cut(s) | 650, 3317 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 4159 |
| Vha464I | CTTAAG | 1 cut(s) | 3578 |
| VneI | GTGCAC | 1 cut(s) | 1792 |
| VpaK11BI | GGWCC | 4 cut(s) | 1634, 3093, 3137, 3649 |
| XapI | RAATTY | 4 cut(s) | 463, 2074, 2798, 3529 |
| XbaI | TCTAGA | 2 cut(s) | 2206, 3290 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 418, 955 |
| XhoI | CTCGAG | 3 cut(s) | 2293, 2490, 4045 |
| XmiI | GTMKAC | 3 cut(s) | 764, 1077, 3912 |
| XmnI | GAANNNNTTC | 5 cut(s) | 1773, 2247, 2563, 2798, 3079 |
| ZraI | GACGTC | 1 cut(s) | 2285 |
| Zsp2I | ATGCAT | 2 cut(s) | 1027, 3758 |