pycom03g22280

CW-type Zinc Finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009959
Physical Location & Seq
Forward (+)
22417540 .. 22418923
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22280.2

Sequence Viewer

Length: 564 bp
ATGACTCAGTGCATGCAAATGTATAGTAGCACCGCAAAACTATGCAAGTTTTGTGCTCATGAATATGAGAAAGCCAAGGATATGGCTGCTGCTGCTTTGGCCTACAAATGCATGGAGGTGGCTTATATGAGGGCGGTATACTGCTCGCATGCTAGTGCAAGCAGAGATCGTCTTGAGTTGCAAACAGCTTTACAATTGGTTCCTCCTGGTGAATCTCCTTCTTCCTCTGCCTCTGATGTTGATAACCTAAACAACCCCTCAACGGTTGACAAGGTTGCCTTACCCAAGGGTGTTAGCTCTCCCCAAGTTGCTGGAAACCATGTTATTGCTGCCCGAAACCGTCCCAATTTCCTCCGGATACTCAATTTTACTCAGGATGTAAATTTTGCAATGGAAGCGTCGAGGAAATCGCGGCTTGCATTTGCAGCTGCTAATACAAACACTGGAGATGCTAAGCGTTCAGAAGGTATTTCTGCCATTAAAAGGGCTCTTGACTTTCACTTCCAAGACGTAGAGGGATTGCTACATTTGGTACGGCTCGCAATGGATGCTATCAGCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CW

Protein Analysis

188

Amino Acids

20.42

Weight (kDa)

8.84

Isoelectric Point (pI)

38.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62900 AT3G62900 AT3G62900
fragaria_vesca FvH4_2g25070
malus_domestica MD03G1282000.v1.1 MD11G1301800.v1.1
prunus_persica Prupe.8G255400_v2.0.a1
pyrus_communis pycom03g22260 pycom03g22270 pycom03g22280 pycom11g26440
rosa_chinensis RchiOBHm_Chr6g0293091
rosa_laevigata RLG00000011972
rosa_multiflora Rmu_sc0001042.1_g000002
rosa_roxburghii Rroxscaffold_7G00174420
rosa_rugosa Rorug06G0232700
rosa_samantha Rh6AG345500 Rh6BG353300 Rh6CG359700 Rh6DG345600
rosa_wichuraiana Rw6G030110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 412
AccIII TCCGGA 1 cut(s) 354
AciI CCGC 3 cut(s) 33, 134, 412
AcsI RAATTY 1 cut(s) 382
AfaI GTAC 1 cut(s) 534
AfiI CCNNNNNNNGG 2 cut(s) 262, 483
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 3 cut(s) 188, 297, 428
AluI AGCT 3 cut(s) 188, 297, 428
Alw21I GWGCWC 1 cut(s) 58
Aor13HI TCCGGA 1 cut(s) 354
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 6 cut(s) 86, 89, 92, 329, 425, 428
ApoI RAATTY 1 cut(s) 382
AsuHPI GGTGA 1 cut(s) 221
BanII GRGCYC 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 58
BbvI GCAGC 6 cut(s) 73, 76, 79, 316, 415, 437
BceAI ACGGC 1 cut(s) 551
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 1 cut(s) 351
BfaI CTAG 1 cut(s) 153
BfuI GTATCC 1 cut(s) 351
BisI GCNGC 7 cut(s) 87, 90, 93, 330, 413, 426, 429
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 7 cut(s) 88, 91, 94, 331, 414, 427, 430
Bme1390I CCNGG 1 cut(s) 207
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 2 cut(s) 439, 538
BpmI CTGGAG 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 75, 285
BsaWI WCCGGW 1 cut(s) 354
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 483
Bse118I RCCGGY 1 cut(s) 558
Bse1I ACTGG 1 cut(s) 448
Bse3DI GCAATG 2 cut(s) 396, 549
BseAI TCCGGA 1 cut(s) 354
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 75, 285
BseGI GGATG 2 cut(s) 382, 553
BseLI CCNNNNNNNGG 2 cut(s) 262, 483
BseMI GCAATG 2 cut(s) 396, 549
BseMII CTCAG 2 cut(s) 20, 386
BseNI ACTGG 1 cut(s) 448
BseXI GCAGC 6 cut(s) 73, 76, 79, 316, 415, 437
Bsh1236I CGCG 1 cut(s) 412
BshFI GGCC 1 cut(s) 101
BsiHKAI GWGCWC 1 cut(s) 58
BsiSI CCGG 2 cut(s) 355, 559
BslFI GGGAC 1 cut(s) 327
BslI CCNNNNNNNGG 2 cut(s) 262, 483
BsmFI GGGAC 1 cut(s) 327
BsnI GGCC 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 58, 490
Bsp13I TCCGGA 1 cut(s) 354
Bsp143I GATC 1 cut(s) 166
Bsp1720I GCTNAGC 1 cut(s) 453
BspACI CCGC 3 cut(s) 33, 134, 412
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 2 cut(s) 19, 385
BspEI TCCGGA 1 cut(s) 354
BspFNI CGCG 1 cut(s) 412
BspHI TCATGA 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 201
BsrDI GCAATG 2 cut(s) 396, 549
BsrFI RCCGGY 1 cut(s) 558
BsrI ACTGG 1 cut(s) 448
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 2 cut(s) 75, 285
BssMI GATC 1 cut(s) 166
BssNAI GTATAC 1 cut(s) 139
BssT1I CCWWGG 2 cut(s) 75, 285
Bst1107I GTATAC 1 cut(s) 139
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 2 cut(s) 265, 341
BstAPI GCANNNNNTGC 1 cut(s) 548
BstC8I GCNNGC 6 cut(s) 14, 146, 150, 160, 417, 540
BstDEI CTNAG 3 cut(s) 6, 372, 453
BstF5I GGATG 2 cut(s) 382, 553
BstFNI CGCG 1 cut(s) 412
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstMWI GCNNNNNNNGC 5 cut(s) 92, 98, 395, 425, 548
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 2 cut(s) 16, 152
BstSCI CCNGG 1 cut(s) 205
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 6 cut(s) 73, 76, 79, 316, 415, 437
BstXI CCANNNNNNTGG 2 cut(s) 82, 311
BstZ17I GTATAC 1 cut(s) 139
BsuI GTATCC 1 cut(s) 351
BsuRI GGCC 1 cut(s) 101
BtsCI GGATG 2 cut(s) 382, 553
BtsIMutI CAGTG 2 cut(s) 14, 441
Cac8I GCNNGC 6 cut(s) 14, 146, 150, 160, 417, 540
CciI TCATGA 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 558
CseI GACGC 1 cut(s) 387
Csp6I GTAC 1 cut(s) 533
CviAII CATG 5 cut(s) 13, 59, 112, 149, 320
CviQI GTAC 1 cut(s) 533
DdeI CTNAG 3 cut(s) 6, 372, 453
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 75, 285
Eco24I GRGCYC 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 2 cut(s) 75, 285
EcoT22I ATGCAT 1 cut(s) 113
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 2 cut(s) 75, 285
FaeI CATG 5 cut(s) 16, 62, 115, 152, 323
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 1 cut(s) 327
FatI CATG 5 cut(s) 12, 58, 111, 148, 319
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 7 cut(s) 87, 90, 93, 330, 413, 426, 429
FokI GGATG 2 cut(s) 389, 560
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 7 cut(s) 87, 90, 93, 330, 413, 426, 429
FspBI CTAG 1 cut(s) 153
GluI GCNGC 7 cut(s) 87, 90, 93, 330, 413, 426, 429
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 1 cut(s) 101
HapII CCGG 2 cut(s) 355, 559
HgaI GACGC 1 cut(s) 387
Hin1II CATG 5 cut(s) 16, 62, 115, 152, 323
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HinfI GANTC 2 cut(s) 4, 212
HpaII CCGG 2 cut(s) 355, 559
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 2 cut(s) 139, 268
Hpy188I TCNGA 2 cut(s) 235, 463
Hpy188III TCNNGA 5 cut(s) 59, 173, 355, 374, 491
Hpy8I GTNNAC 2 cut(s) 139, 268
Hpy99I CGWCG 1 cut(s) 403
HpyAV CCTTC 2 cut(s) 228, 458
HpyCH4III ACNGT 2 cut(s) 265, 341
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 9 cut(s) 12, 16, 45, 111, 158, 181, 389, 419, 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 98, 395, 425, 548
HpyF3I CTNAG 3 cut(s) 6, 372, 453
HpySE526I ACGT 1 cut(s) 510
Hsp92II CATG 5 cut(s) 16, 62, 115, 152, 323
Kpn2I TCCGGA 1 cut(s) 354
Kzo9I GATC 1 cut(s) 166
LpnPI CCDG 6 cut(s) 192, 219, 297, 359, 368, 429
Lsp1109I GCAGC 6 cut(s) 73, 76, 79, 316, 415, 437
LweI GCATC 2 cut(s) 439, 538
MaeI CTAG 1 cut(s) 153
MaeII ACGT 1 cut(s) 510
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 1 cut(s) 213
MfeI CAATTG 1 cut(s) 194
MhlI GDGCHC 2 cut(s) 58, 490
MluCI AATT 4 cut(s) 194, 346, 364, 382
MnlI CCTC 9 cut(s) 109, 123, 213, 235, 241, 268, 362, 396, 508
Mph1103I ATGCAT 1 cut(s) 113
MroI TCCGGA 1 cut(s) 354
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 4 cut(s) 17, 63, 116, 153
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 2 cut(s) 355, 559
MspR9I CCNGG 1 cut(s) 207
MunI CAATTG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 207
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 5 cut(s) 92, 98, 395, 425, 548
NdeII GATC 1 cut(s) 166
NlaIII CATG 5 cut(s) 16, 62, 115, 152, 323
NlaIV GGNNCC 1 cut(s) 201
NsiI ATGCAT 1 cut(s) 113
NspI RCATGY 2 cut(s) 16, 152
PaeI GCATGC 2 cut(s) 16, 152
PagI TCATGA 1 cut(s) 58
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 7 cut(s) 88, 91, 94, 331, 414, 427, 430
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 201
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 1 cut(s) 534
RsaNI GTAC 1 cut(s) 533
RseI CAYNNNNRTG 4 cut(s) 17, 63, 116, 153
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 7 cut(s) 87, 90, 93, 330, 413, 426, 429
Sau3AI GATC 1 cut(s) 166
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 2 cut(s) 58, 490
SetI ASST 8 cut(s) 120, 190, 249, 276, 299, 430, 469, 513
SfaNI GCATC 2 cut(s) 439, 538
SmiMI CAYNNNNRTG 4 cut(s) 17, 63, 116, 153
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
SphI GCATGC 2 cut(s) 16, 152
Sse9I AATT 4 cut(s) 194, 346, 364, 382
SsiI CCGC 3 cut(s) 33, 134, 412
SspMI CTAG 1 cut(s) 153
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 2 cut(s) 75, 285
TaaI ACNGT 2 cut(s) 265, 341
TaiI ACGT 1 cut(s) 513
TaqI TCGA 1 cut(s) 401
TasI AATT 4 cut(s) 194, 346, 364, 382
TauI GCSGC 1 cut(s) 415
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 2 cut(s) 14, 448
TseI GCWGC 6 cut(s) 86, 89, 92, 329, 425, 428
TspDTI ATGAA 1 cut(s) 75
TspRI CASTG 2 cut(s) 14, 448
XapI RAATTY 1 cut(s) 382
XceI RCATGY 2 cut(s) 16, 152
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 1 cut(s) 153
Zsp2I ATGCAT 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.