pycom03g22260

CW-type Zinc Finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009959
Physical Location & Seq
Forward (+)
22411539 .. 22414363
2825 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22260.4

Sequence Viewer

Length: 1599 bp
ATGATTTCTGTGGGGACTAGGGATGCAAGGAAGGGGGTAGAATTAGGGTTTGGTGGTCGGAGAGAGATGGAGGATACTGAGCTTGAAGAAGGCGAAGCTTGCTCTTCCCACATCAATGAATACGATCCCAACATCGATGCTCTCTCTTACATCGATGATAGAATTCAGGATGTTTTGGGACACTTCCAGAAAGAATTTGAAGGTGAGGTATCTGCGGAGAATTTGGGGGCAAAGTGGGGTGGGTATGGCTCTTTTTTACCTTCCTATCAGCGGTCTCCAGTGTGGTCTCATCCGAAGACTCCACAAAAAGTTCACAACTACAGCTTACTGAAATCTCCCAACAATTTGAAACTTGAGTCTGGTCAACGTAACAATGCAGTTTGTTATAATACACCTCAGTCGGTGGGAGTAGGAACTGCTTCTACCGGTTCCACTTCGCTTGTTGTACCAAAGGCACCCTCATTAGTTCAACCTGACCAGTACGCTCCTCGGCATGAATCTGCAAACAAGAAAGCTATCAATTCATCAGACCAGAAAACATTGAAGGTGCGAATTAAAGTGGGATCAGATAACTTGTCGACACGAAAAAATGCTATCTACAGTGGGCTTGGCCTTGATACCACACCGTCTTCCTCACTAGATGATAGCCCTTCAGAGAGTGAAGGGATATCTCATGAGCCTCGAGATGCCCCATTTGAATCTCCCACCAGTATTCTTCAGATTATGACATCCTTTCCGGTGCATGAGGATGTGATGTCACCTCTTCATGACGACCTCATTTACTTAATTGCAAAGGAAAAGCTCCTAAAAGAGGGTACAGCGAATGGAACTCATACCATGGAGGGTGGTGGGAAAGTATCAAGAGTGAGGAAAACAAAATCGGTTGAAAGAAATGATTTGTCAGTAGAATCAAAAAGTGGGAGAAATAAGGATGGAACTGGGCTTCTGTCAAAGAAGGAACACGACATTGACATGTTTGCTTGTGAGGAGTTTGTTTCTAAAACCTTGAAGCTCCCGCTTCTATCTAATTCATTTTCTACTGTCAATGACGTGATCAAGAGCAATGAAATTGATAAGAAAAGTTTAGTGAGGGACAAGGTCTTCCCTGCAGAAGACGAACCAATGGAGCGAATGTCCAACCAAGAGGATGGCTGGGTTGAAAAGCGAAAAGCCAATTTGGCTGGAAAGGTTCAGGAAGATAGAAAAGTAAATTTAAGCGATGATGTTTTGGCTCATCCAAAGAAAGAGGGCTGTTGCAGAGGAGAGAAAACTTATGAATTGGTAAAAGGTGACTTAAATGTTTCCAAGGGGAGGAAAGCTTTAAATACTGAAATCATGGACCATTCAAAGCAGAAAGTCAATCAGAAGGCTGGATTGCATGAGGTGGATGATACAAGACTATATTCTGGGAAGGAGTATCCGCTACCCGGGGAAAAAAAGAAACCGAAGGAAAGTCAGAGGACCCCAGTTGCTGAGATGCCAAAAGAAAGCTCAAGGGTTTGTTCTTCTTCAGCGCCCAAGATGAAGAGCACGCATGCGAACAGTTCTAATACTGATCAAAGTAGGGATACATATAGATCGATCTGTTTGGGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-CW

Protein Analysis

533

Amino Acids

58.76

Weight (kDa)

6.73

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009959)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62900 AT3G62900 AT3G62900
fragaria_vesca FvH4_2g25070
malus_domestica MD03G1282000.v1.1 MD11G1301800.v1.1
prunus_persica Prupe.8G255400_v2.0.a1
pyrus_communis pycom03g22260 pycom03g22270 pycom03g22280 pycom11g26440
rosa_chinensis RchiOBHm_Chr6g0293091
rosa_laevigata RLG00000011972
rosa_multiflora Rmu_sc0001042.1_g000002
rosa_roxburghii Rroxscaffold_7G00174420
rosa_rugosa Rorug06G0232700
rosa_samantha Rh6AG345500 Rh6BG353300 Rh6CG359700 Rh6DG345600
rosa_wichuraiana Rw6G030110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AccB1I GGYRCC 1 cut(s) 454
AccI GTMKAC 1 cut(s) 578
AciI CCGC 4 cut(s) 215, 271, 1016, 1421
AclWI GGATC 2 cut(s) 119, 571
AcsI RAATTY 4 cut(s) 162, 194, 220, 1210
AcuI CTGAAG 3 cut(s) 636, 701, 1494
AfaI GTAC 3 cut(s) 447, 482, 817
AfiI CCNNNNNNNGG 4 cut(s) 270, 811, 1311, 1427
AflIII ACRYGT 1 cut(s) 972
AgeI ACCGGT 1 cut(s) 425
AjiI CACGTC 1 cut(s) 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 33, 65
AluBI AGCT 8 cut(s) 82, 98, 324, 515, 802, 1012, 1319, 1491
AluI AGCT 8 cut(s) 82, 98, 324, 515, 802, 1012, 1319, 1491
Alw21I GWGCWC 1 cut(s) 1532
Alw26I GTCTC 2 cut(s) 279, 291
AlwI GGATC 2 cut(s) 119, 571
AlwNI CAGNNNCTG 1 cut(s) 1472
Ama87I CYCGRG 2 cut(s) 681, 1427
AoxI GGCC 1 cut(s) 610
ApoI RAATTY 4 cut(s) 162, 194, 220, 1210
AsiGI ACCGGT 1 cut(s) 425
Asp700I GAANNNNTTC 1 cut(s) 418
AspLEI GCGC 1 cut(s) 1516
AspS9I GGNCC 2 cut(s) 1339, 1461
AsuC2I CCSGG 2 cut(s) 1428, 1429
AsuHPI GGTGA 3 cut(s) 215, 750, 1301
AvaI CYCGRG 2 cut(s) 681, 1427
AvaII GGWCC 2 cut(s) 1339, 1461
BanI GGYRCC 1 cut(s) 454
BbsI GAAGAC 4 cut(s) 302, 621, 1093, 1119
Bbv12I GWGCWC 1 cut(s) 1532
BccI CCATC 3 cut(s) 61, 926, 1142
BciVI GTATCC 3 cut(s) 67, 1428, 1561
BclI TGATCA 2 cut(s) 1053, 1555
BcnI CCSGG 2 cut(s) 1428, 1429
BcoDI GTCTC 2 cut(s) 279, 291
BfaI CTAG 2 cut(s) 18, 638
BfmI CTRYAG 3 cut(s) 319, 598, 1107
BfoI RGCGCY 1 cut(s) 1517
BfuI GTATCC 3 cut(s) 67, 1428, 1561
BglI GCCNNNNNGGC 1 cut(s) 1178
Bme1390I CCNGG 2 cut(s) 1428, 1429
Bme18I GGWCC 2 cut(s) 1339, 1461
BmeT110I CYCGRG 2 cut(s) 681, 1427
BmgBI CACGTC 1 cut(s) 1051
BmgT120I GGNCC 2 cut(s) 1339, 1461
BmiI GGNNCC 3 cut(s) 430, 456, 1463
BmrFI CCNGG 2 cut(s) 1428, 1429
BmrI ACTGGG 2 cut(s) 948, 1460
BmsI GCATC 4 cut(s) 13, 127, 676, 1467
BmuI ACTGGG 2 cut(s) 948, 1460
BpiI GAAGAC 4 cut(s) 302, 621, 1093, 1119
BpmI CTGGAG 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 374, 1477
BpuMI CCSGG 2 cut(s) 1428, 1429
Bsa29I ATCGAT 3 cut(s) 135, 153, 1580
BsaI GGTCTC 2 cut(s) 279, 291
BsaJI CCNNGG 5 cut(s) 488, 837, 1305, 1427, 1428
BsaWI WCCGGW 2 cut(s) 425, 736
BsaXI ACNNNNNCTCC 2 cut(s) 1118, 1148
Bsc4I CCNNNNNNNGG 4 cut(s) 270, 811, 1311, 1427
Bse118I RCCGGY 1 cut(s) 425
Bse1I ACTGG 5 cut(s) 278, 478, 708, 943, 1466
Bse3DI GCAATG 1 cut(s) 1069
BseCI ATCGAT 3 cut(s) 135, 153, 1580
BseDI CCNNGG 5 cut(s) 488, 837, 1305, 1427, 1428
BseLI CCNNNNNNNGG 4 cut(s) 270, 811, 1311, 1427
BseMI GCAATG 1 cut(s) 1069
BseMII CTCAG 3 cut(s) 69, 410, 1464
BseNI ACTGG 5 cut(s) 278, 478, 708, 943, 1466
BseRI GAGGAG 3 cut(s) 477, 1001, 1275
BseYI CCCAGC 1 cut(s) 1152
BshFI GGCC 1 cut(s) 612
BshNI GGYRCC 1 cut(s) 454
BshTI ACCGGT 1 cut(s) 425
BshVI ATCGAT 3 cut(s) 135, 153, 1580
BsiHKAI GWGCWC 1 cut(s) 1532
BsiHKCI CYCGRG 2 cut(s) 681, 1427
BsiSI CCGG 3 cut(s) 426, 737, 1428
BslFI GGGAC 3 cut(s) 28, 192, 1106
BslI CCNNNNNNNGG 4 cut(s) 270, 811, 1311, 1427
BsmAI GTCTC 2 cut(s) 279, 291
BsmFI GGGAC 3 cut(s) 28, 192, 1106
BsnI GGCC 1 cut(s) 612
Bso31I GGTCTC 2 cut(s) 279, 291
BsoBI CYCGRG 2 cut(s) 681, 1427
Bsp1286I GDGCHC 1 cut(s) 1532
Bsp143I GATC 6 cut(s) 124, 563, 1053, 1555, 1577, 1581
Bsp19I CCATGG 1 cut(s) 837
BspACI CCGC 4 cut(s) 215, 271, 1016, 1421
BspANI GGCC 1 cut(s) 612
BspCNI CTCAG 3 cut(s) 70, 409, 1465
BspDI ATCGAT 3 cut(s) 135, 153, 1580
BspHI TCATGA 2 cut(s) 673, 766
BspLI GGNNCC 3 cut(s) 430, 456, 1463
BspMAI CTGCAG 1 cut(s) 1111
BspPI GGATC 2 cut(s) 119, 571
BspQI GCTCTTC 2 cut(s) 109, 1520
BspT107I GGYRCC 1 cut(s) 454
BspTNI GGTCTC 2 cut(s) 279, 291
BsrDI GCAATG 1 cut(s) 1069
BsrFI RCCGGY 1 cut(s) 425
BsrI ACTGG 5 cut(s) 278, 478, 708, 943, 1466
BssAI RCCGGY 1 cut(s) 425
BssECI CCNNGG 5 cut(s) 488, 837, 1305, 1427, 1428
BssMI GATC 6 cut(s) 124, 563, 1053, 1555, 1577, 1581
BssT1I CCWWGG 2 cut(s) 837, 1305
Bst4CI ACNGT 4 cut(s) 602, 627, 1042, 1544
Bst6I CTCTTC 3 cut(s) 109, 768, 1520
BstC8I GCNNGC 3 cut(s) 100, 1532, 1536
BstDEI CTNAG 3 cut(s) 78, 396, 1473
BstDSI CCRYGG 1 cut(s) 837
BstENI CCTNNNNNAGG 1 cut(s) 809
BstH2I RGCGCY 1 cut(s) 1517
BstHHI GCGC 1 cut(s) 1516
BstKTI GATC 6 cut(s) 127, 566, 1056, 1558, 1580, 1584
BstMAI GTCTC 2 cut(s) 279, 291
BstMBI GATC 6 cut(s) 124, 563, 1053, 1555, 1577, 1581
BstMWI GCNNNNNNNGC 2 cut(s) 99, 1178
BstNSI RCATGY 2 cut(s) 976, 1538
BstSCI CCNGG 2 cut(s) 1426, 1427
BstSFI CTRYAG 3 cut(s) 319, 598, 1107
BstV2I GAAGAC 4 cut(s) 302, 621, 1093, 1119
BstXI CCANNNNNNTGG 1 cut(s) 1148
Bsu15I ATCGAT 3 cut(s) 135, 153, 1580
BsuI GTATCC 3 cut(s) 67, 1428, 1561
BsuRI GGCC 1 cut(s) 612
BsuTUI ATCGAT 3 cut(s) 135, 153, 1580
BtgI CCRYGG 1 cut(s) 837
BtgZI GCGATG 1 cut(s) 1233
BtrI CACGTC 1 cut(s) 1051
BtsIMutI CAGTG 2 cut(s) 285, 607
Cac8I GCNNGC 3 cut(s) 100, 1532, 1536
CaiI CAGNNNCTG 1 cut(s) 1472
CciI TCATGA 2 cut(s) 673, 766
CfoI GCGC 1 cut(s) 1516
Cfr10I RCCGGY 1 cut(s) 425
Cfr13I GGNCC 2 cut(s) 1339, 1461
Cfr9I CCCGGG 1 cut(s) 1427
ClaI ATCGAT 3 cut(s) 135, 153, 1580
Csp6I GTAC 3 cut(s) 446, 481, 816
CspAI ACCGGT 1 cut(s) 425
CspCI CAANNNNNGTGG 2 cut(s) 421, 456
CviAII CATG 9 cut(s) 494, 674, 743, 767, 838, 973, 1336, 1379, 1535
CviQI GTAC 3 cut(s) 446, 481, 816
DdeI CTNAG 3 cut(s) 78, 396, 1473
DpnI GATC 6 cut(s) 126, 565, 1055, 1557, 1579, 1583
DpnII GATC 6 cut(s) 124, 563, 1053, 1555, 1577, 1581
DraI TTTAAA 1 cut(s) 1323
Eam1104I CTCTTC 3 cut(s) 109, 768, 1520
EarI CTCTTC 3 cut(s) 109, 768, 1520
Eco130I CCWWGG 2 cut(s) 837, 1305
Eco31I GGTCTC 2 cut(s) 279, 291
Eco32I GATATC 1 cut(s) 669
Eco47I GGWCC 2 cut(s) 1339, 1461
Eco57I CTGAAG 3 cut(s) 636, 701, 1494
Eco88I CYCGRG 2 cut(s) 681, 1427
EcoNI CCTNNNNNAGG 1 cut(s) 809
EcoO109I RGGNCCY 1 cut(s) 1461
EcoRI GAATTC 1 cut(s) 162
EcoRV GATATC 1 cut(s) 669
EcoT14I CCWWGG 2 cut(s) 837, 1305
ErhI CCWWGG 2 cut(s) 837, 1305
FaeI CATG 9 cut(s) 497, 677, 746, 770, 841, 976, 1339, 1382, 1538
FaqI GGGAC 3 cut(s) 28, 192, 1106
FatI CATG 9 cut(s) 493, 673, 742, 766, 837, 972, 1335, 1378, 1534
FauI CCCGC 1 cut(s) 1023
FbaI TGATCA 2 cut(s) 1053, 1555
FblI GTMKAC 1 cut(s) 578
FokI GGATG 9 cut(s) 35, 182, 276, 715, 761, 944, 1160, 1221, 1400
FspBI CTAG 2 cut(s) 18, 638
GlaI GCGC 1 cut(s) 1515
GsaI CCCAGC 1 cut(s) 1156
GsuI CTGGAG 1 cut(s) 261
HaeII RGCGCY 1 cut(s) 1517
HaeIII GGCC 1 cut(s) 612
HapII CCGG 3 cut(s) 426, 737, 1428
HhaI GCGC 1 cut(s) 1516
Hin1II CATG 9 cut(s) 497, 677, 746, 770, 841, 976, 1339, 1382, 1538
Hin6I GCGC 1 cut(s) 1514
HinP1I GCGC 1 cut(s) 1514
HincII GTYRAC 2 cut(s) 365, 579
HindII GTYRAC 2 cut(s) 365, 579
HindIII AAGCTT 2 cut(s) 96, 1317
HinfI GANTC 5 cut(s) 298, 356, 497, 698, 908
HpaII CCGG 3 cut(s) 426, 737, 1428
HphI GGTGA 3 cut(s) 215, 750, 1301
Hpy166II GTNNAC 3 cut(s) 313, 365, 579
Hpy188I TCNGA 8 cut(s) 60, 294, 529, 568, 655, 720, 1365, 1458
Hpy188III TCNNGA 8 cut(s) 167, 187, 674, 683, 767, 861, 1057, 1193
Hpy8I GTNNAC 3 cut(s) 313, 365, 579
HpyCH4III ACNGT 4 cut(s) 602, 627, 1042, 1544
HpyCH4IV ACGT 2 cut(s) 367, 1050
HpyCH4V TGCA 8 cut(s) 26, 377, 503, 742, 791, 1109, 1257, 1378
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 1178
HpyF3I CTNAG 3 cut(s) 78, 396, 1473
HpySE526I ACGT 2 cut(s) 367, 1050
Hsp92II CATG 9 cut(s) 497, 677, 746, 770, 841, 976, 1339, 1382, 1538
HspAI GCGC 1 cut(s) 1514
Ksp22I TGATCA 2 cut(s) 1053, 1555
Kzo9I GATC 6 cut(s) 124, 563, 1053, 1555, 1577, 1581
LguI GCTCTTC 2 cut(s) 109, 1520
LmnI GCTCC 4 cut(s) 490, 807, 1017, 1126
LweI GCATC 4 cut(s) 13, 127, 676, 1467
MaeI CTAG 2 cut(s) 18, 638
MaeII ACGT 2 cut(s) 367, 1050
MaeIII GTNAC 3 cut(s) 368, 756, 1289
MalI GATC 6 cut(s) 126, 565, 1055, 1557, 1579, 1583
MboI GATC 6 cut(s) 124, 563, 1053, 1555, 1577, 1581
MhlI GDGCHC 1 cut(s) 1532
MlyI GAGTC 2 cut(s) 292, 365
MmeI TCCRAC 2 cut(s) 38, 1161
MroXI GAANNNNTTC 1 cut(s) 418
MseI TTAA 5 cut(s) 555, 785, 1214, 1295, 1322
MslI CAYNNNNRTG 3 cut(s) 114, 747, 971
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 3 cut(s) 426, 737, 1428
MspR9I CCNGG 2 cut(s) 1428, 1429
MwoI GCNNNNNNNGC 2 cut(s) 99, 1178
NciI CCSGG 2 cut(s) 1428, 1429
NcoI CCATGG 1 cut(s) 837
NdeII GATC 6 cut(s) 124, 563, 1053, 1555, 1577, 1581
NlaIII CATG 9 cut(s) 497, 677, 746, 770, 841, 976, 1339, 1382, 1538
NlaIV GGNNCC 3 cut(s) 430, 456, 1463
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 2 cut(s) 756, 1289
NspI RCATGY 2 cut(s) 976, 1538
PaeI GCATGC 1 cut(s) 1538
PaeR7I CTCGAG 1 cut(s) 681
PagI TCATGA 2 cut(s) 673, 766
PciI ACATGT 1 cut(s) 972
PciSI GCTCTTC 2 cut(s) 109, 1520
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 3 cut(s) 497, 698, 908
PflFI GACNNNGTC 1 cut(s) 1097
PinAI ACCGGT 1 cut(s) 425
PleI GAGTC 2 cut(s) 292, 364
PpsI GAGTC 2 cut(s) 292, 364
PpuMI RGGWCCY 1 cut(s) 1461
PscI ACATGT 1 cut(s) 972
PsiI TTATAA 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 1461
PspFI CCCAGC 1 cut(s) 1152
PspN4I GGNNCC 3 cut(s) 430, 456, 1463
PspPI GGNCC 2 cut(s) 1339, 1461
PspPPI RGGWCCY 1 cut(s) 1461
PsrI GAACNNNNNNTAC 2 cut(s) 406, 438
PstI CTGCAG 1 cut(s) 1111
PstNI CAGNNNCTG 1 cut(s) 1472
PsyI GACNNNGTC 1 cut(s) 1097
RsaI GTAC 3 cut(s) 447, 482, 817
RsaNI GTAC 3 cut(s) 446, 481, 816
RseI CAYNNNNRTG 3 cut(s) 114, 747, 971
SalI GTCGAC 1 cut(s) 577
SapI GCTCTTC 2 cut(s) 109, 1520
SaqAI TTAA 5 cut(s) 555, 785, 1214, 1295, 1322
Sau3AI GATC 6 cut(s) 124, 563, 1053, 1555, 1577, 1581
Sau96I GGNCC 2 cut(s) 1339, 1461
SchI GAGTC 2 cut(s) 292, 365
ScrFI CCNGG 2 cut(s) 1428, 1429
SduI GDGCHC 1 cut(s) 1532
SfaNI GCATC 4 cut(s) 13, 127, 676, 1467
SfcI CTRYAG 3 cut(s) 319, 598, 1107
Sfr274I CTCGAG 1 cut(s) 681
SinI GGWCC 2 cut(s) 1339, 1461
SlaI CTCGAG 1 cut(s) 681
SmaI CCCGGG 1 cut(s) 1429
SmiMI CAYNNNNRTG 3 cut(s) 114, 747, 971
SmlI CTYRAG 3 cut(s) 353, 681, 1492
SmoI CTYRAG 3 cut(s) 353, 681, 1492
SphI GCATGC 1 cut(s) 1538
SsiI CCGC 4 cut(s) 215, 271, 1016, 1421
SspMI CTAG 2 cut(s) 18, 638
StyD4I CCNGG 2 cut(s) 1426, 1427
StyI CCWWGG 2 cut(s) 837, 1305
TaaI ACNGT 4 cut(s) 602, 627, 1042, 1544
TaiI ACGT 2 cut(s) 370, 1053
TaqI TCGA 5 cut(s) 135, 153, 578, 682, 1580
TfiI GAWTC 3 cut(s) 497, 698, 908
Tru1I TTAA 5 cut(s) 555, 785, 1214, 1295, 1322
Tru9I TTAA 5 cut(s) 555, 785, 1214, 1295, 1322
TscAI CASTG 2 cut(s) 285, 607
TseFI GTSAC 2 cut(s) 756, 1289
Tsp45I GTSAC 2 cut(s) 756, 1289
TspDTI ATGAA 8 cut(s) 132, 510, 513, 755, 1020, 1080, 1290, 1538
TspMI CCCGGG 1 cut(s) 1427
TspRI CASTG 2 cut(s) 285, 607
Tth111I GACNNNGTC 1 cut(s) 1097
VpaK11BI GGWCC 2 cut(s) 1339, 1461
XagI CCTNNNNNAGG 1 cut(s) 809
XapI RAATTY 4 cut(s) 162, 194, 220, 1210
XceI RCATGY 2 cut(s) 976, 1538
XhoI CTCGAG 1 cut(s) 681
XmaI CCCGGG 1 cut(s) 1427
XmiI GTMKAC 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 418
XspI CTAG 2 cut(s) 18, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.