AT4G00490

beta-amylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006678
Physical Location & Seq
Forward (+)
222289 .. 225131
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00490.1

Sequence Viewer

Length: 1629 bp
ATGGCGATTAGGTTGAATCATAGTGTAATTCCAGTGAGCGTGAAGCTTGGAGCTCCGACGAGAGTATCGGCTCGGAGCTCGTTACCGTTCAGCGTTGGAGATTGGCGTGGTGTTTCTACATTTTCCGGTGCTAGGCCTTTGGTTCTCGCGAAGGTGAAACTGAGAGCAGAGAGTACTGAGGAAGATCGAGTTCCAATTGATGACGACGACGATTCTACTGATCAGCTCGTTGATGAAGAAATTGTGCATTTCGAAGAGCGTGATTTTGCTGGCACAGCGTGTGTTCCTGTATATGTTATGCTACCACTGGGAGTGATTGATATGAACTCGGAAGTGGTGGAACCGGAGGAGCTTTTGGATCAGTTAAGAACTTTAAAGTCGGTTAATGTTGATGGTGTTATGGTTGACTGCTGGTGGGGAATTGTTGAGTCACATACACCACAGGTTTATAACTGGAGTGGTTACAAGAAACTTTTTCAGATGATTCGTGAGCTTGGACTTAAGATTCAGGTTGTTATGTCGTTTCACGAATGTGGAGGCAATGTTGGAGATGATGTGCATATACAAATCCCTGAGTGGGTGAGAGAAATTGGTCAAAGCAATCCTGACATCTACTTCACTGACAGTGCTGGCAGGCGTAACACTGAATGCCTTACTTGGGGAATTGACAAGCAACGTGTTTTAAGAGGACGGACTGCTCTTGAGGTTTACTTTGATTACATGAGAAGCTTTCGTGTGGAATTTGATGAATTCTTTGAGGAGAAAATCATCCCTGAAATTGAAGTTGGACTAGGTCCATGTGGGGAGCTGAGGTATCCTTCATACCCTGCACAATTTGGGTGGAAATACCCTGGTATTGGTGAATTCCAGTGTTATGATAAATATTTGATGAATAGCTTAAAGGAGGCTGCAGAAGTAAGAGGCCACAGCTTTTGGGGCAGAGGACCTGATAACACAGAGACTTATAATTCAACACCACATGGGACTGGATTTTTTCGCGATGGAGGCGATTATGACAGCTACTATGGTAGATTCTTTCTTAATTGGTACTCAAGAGTTCTGATTGACCATGGTGATCGTGTTCTTGCTATGGCAAATTTGGCTTTCGAAGGAACTTGCATTGCTGCAAAGCTCTCAGGTATACACTGGTGGTATAAAACAGCTAGTCATGCTGCTGAACTCACTGCTGGCTTCTACAACTCCTCAAACCGTGATGGCTATGGTCCAATAGCTGCAATGTTTAAGAAACATGATGCTGCTCTCAACTTCACATGTGTGGAGTTACGGACACTTGATCAACATGAGGATTTCCCAGAGGCTCTGGCAGATCCAGAAGGTCTAGTTTGGCAGGTGCTGAATGCTGCTTGGGATGCTAGTATACCTGTTGCTAGTGAAAATGCTCTTCCTTGTTATGACAGAGAAGGTTACAACAAGATACTTGAAAACGCAAAGCCCCTTACCGATCCTGATGGTCGCCACCTTTCATGTTTCACTTACCTGAGGCTTAACCCAACTCTGATGGAGTCTCAAAACTTCAAAGAGTTTGAGAGATTCCTCAAACGAATGCATGGTGAAGCCGTTCCGGATCTTGGTTTGGCTCCAGGGACACAAGAGACCAACCCCGAGTGA

Protein Analysis

542

Amino Acids

61.4

Weight (kDa)

4.89

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 98 - 517 1.6e-133 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 450, 966
AarI CACCTGC 1 cut(s) 1339
Acc36I ACCTGC 1 cut(s) 1339
AccI GTMKAC 2 cut(s) 1141, 1378
AccII CGCG 2 cut(s) 149, 999
AccIII TCCGGA 1 cut(s) 1582
AclWI GGATC 4 cut(s) 366, 1322, 1457, 1593
AcsI RAATTY 4 cut(s) 740, 749, 863, 1096
AdeI CACNNNGTG 1 cut(s) 279
AfaI GTAC 2 cut(s) 175, 1049
AfiI CCNNNNNNNGG 5 cut(s) 132, 577, 658, 857, 1589
AflII CTTAAG 1 cut(s) 500
AflIII ACRYGT 2 cut(s) 676, 1271
AgsI TTSAA 5 cut(s) 16, 782, 972, 1442, 1537
AjnI CCWGG 2 cut(s) 850, 1600
AjuI GAANNNNNNNTTGG 2 cut(s) 768, 800
AleI CACNNNNGTG 2 cut(s) 531, 1274
Alw21I GWGCWC 2 cut(s) 55, 80
Alw26I GTCTC 3 cut(s) 953, 1530, 1607
AlwI GGATC 4 cut(s) 366, 1322, 1457, 1593
AlwNI CAGNNNCTG 1 cut(s) 1354
Ama87I CYCGRG 1 cut(s) 1622
Aor13HI TCCGGA 1 cut(s) 1582
AoxI GGCC 2 cut(s) 134, 922
ApeKI GCWGC 6 cut(s) 908, 1124, 1172, 1232, 1256, 1361
ApoI RAATTY 4 cut(s) 740, 749, 863, 1096
Asp700I GAANNNNTTC 1 cut(s) 1578
AspS9I GGNCC 3 cut(s) 794, 944, 1223
AsuHPI GGTGA 5 cut(s) 166, 592, 872, 1085, 1583
AsuII TTCGAA 2 cut(s) 252, 1107
AvaI CYCGRG 1 cut(s) 1622
AvaII GGWCC 3 cut(s) 794, 944, 1223
AxyI CCTNAGG 1 cut(s) 1499
BaeI ACNNNNGTAYC 2 cut(s) 48, 81
BanII GRGCYC 2 cut(s) 55, 80
Bbv12I GWGCWC 2 cut(s) 55, 80
BbvCI CCTCAGC 1 cut(s) 809
BbvI GCAGC 6 cut(s) 895, 1111, 1159, 1219, 1243, 1348
BccI CCATC 5 cut(s) 386, 995, 1208, 1463, 1513
BceAI ACGGC 1 cut(s) 1562
BciT130I CCWGG 2 cut(s) 852, 1602
BciVI GTATCC 1 cut(s) 825
BclI TGATCA 2 cut(s) 220, 1294
BcoDI GTCTC 3 cut(s) 953, 1530, 1607
BfaI CTAG 6 cut(s) 132, 791, 1164, 1340, 1374, 1389
BfmI CTRYAG 1 cut(s) 909
BfrI CTTAAG 1 cut(s) 500
BfuAI ACCTGC 1 cut(s) 1339
BfuI GTATCC 1 cut(s) 825
BisI GCNGC 6 cut(s) 909, 1125, 1173, 1233, 1257, 1362
BlsI GCNGC 6 cut(s) 910, 1126, 1174, 1234, 1258, 1363
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 2 cut(s) 852, 1602
Bme18I GGWCC 3 cut(s) 794, 944, 1223
BmeT110I CYCGRG 1 cut(s) 1622
BmgT120I GGNCC 3 cut(s) 794, 944, 1223
BmiI GGNNCC 2 cut(s) 342, 1599
BmrFI CCNGG 2 cut(s) 852, 1602
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 2 cut(s) 1243, 1360
BmuI ACTGGG 1 cut(s) 317
BpmI CTGGAG 2 cut(s) 475, 1584
Bpu10I CCTNAGC 1 cut(s) 809
Bpu14I TTCGAA 2 cut(s) 252, 1107
BpuEI CTTGAG 2 cut(s) 722, 1036
BsaI GGTCTC 1 cut(s) 1607
BsaJI CCNNGG 3 cut(s) 850, 1069, 1601
BsaWI WCCGGW 3 cut(s) 125, 343, 1582
BsaXI ACNNNNNCTCC 6 cut(s) 528, 540, 558, 570, 797, 827
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 577, 658, 857, 1589
Bse1I ACTGG 6 cut(s) 32, 312, 458, 868, 991, 1151
Bse21I CCTNAGG 1 cut(s) 1499
Bse3DI GCAATG 3 cut(s) 547, 1119, 1242
BseAI TCCGGA 1 cut(s) 1582
BseBI CCWGG 2 cut(s) 852, 1602
BseDI CCNNGG 3 cut(s) 850, 1069, 1601
BseGI GGATG 2 cut(s) 768, 1375
BseLI CCNNNNNNNGG 5 cut(s) 132, 577, 658, 857, 1589
BseMI GCAATG 3 cut(s) 547, 1119, 1242
BseMII CTCAG 6 cut(s) 152, 168, 564, 800, 1149, 1490
BseNI ACTGG 6 cut(s) 32, 312, 458, 868, 991, 1151
BseRI GAGGAG 3 cut(s) 362, 773, 1192
BseXI GCAGC 6 cut(s) 895, 1111, 1159, 1219, 1243, 1348
BsgI GTGCAG 1 cut(s) 813
Bsh1236I CGCG 2 cut(s) 149, 999
BshFI GGCC 2 cut(s) 136, 924
BsiHKAI GWGCWC 2 cut(s) 55, 80
BsiHKCI CYCGRG 1 cut(s) 1622
BsiSI CCGG 3 cut(s) 126, 344, 1583
BslFI GGGAC 2 cut(s) 997, 1618
BslI CCNNNNNNNGG 5 cut(s) 132, 577, 658, 857, 1589
BsmAI GTCTC 3 cut(s) 953, 1530, 1607
BsmFI GGGAC 2 cut(s) 997, 1618
BsmI GAATGC 3 cut(s) 653, 1363, 1569
BsnI GGCC 2 cut(s) 136, 924
Bso31I GGTCTC 1 cut(s) 1607
BsoBI CYCGRG 1 cut(s) 1622
Bsp119I TTCGAA 2 cut(s) 252, 1107
Bsp1286I GDGCHC 2 cut(s) 55, 80
Bsp13I TCCGGA 1 cut(s) 1582
Bsp143I GATC 8 cut(s) 184, 220, 358, 1075, 1294, 1327, 1462, 1585
Bsp19I CCATGG 1 cut(s) 1069
Bsp68I TCGCGA 2 cut(s) 149, 999
BspANI GGCC 2 cut(s) 136, 924
BspCNI CTCAG 6 cut(s) 153, 169, 565, 801, 1148, 1491
BspEI TCCGGA 1 cut(s) 1582
BspFNI CGCG 2 cut(s) 149, 999
BspLI GGNNCC 2 cut(s) 342, 1599
BspMAI CTGCAG 1 cut(s) 913
BspMI ACCTGC 1 cut(s) 1339
BspPI GGATC 4 cut(s) 366, 1322, 1457, 1593
BspQI GCTCTTC 2 cut(s) 249, 1407
BspT104I TTCGAA 2 cut(s) 252, 1107
BspTI CTTAAG 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 1607
BsrDI GCAATG 3 cut(s) 547, 1119, 1242
BsrI ACTGG 6 cut(s) 32, 312, 458, 868, 991, 1151
BssECI CCNNGG 3 cut(s) 850, 1069, 1601
BssMI GATC 8 cut(s) 184, 220, 358, 1075, 1294, 1327, 1462, 1585
BssNAI GTATAC 2 cut(s) 1142, 1379
BssT1I CCWWGG 1 cut(s) 1069
Bst1107I GTATAC 2 cut(s) 1142, 1379
Bst2UI CCWGG 2 cut(s) 852, 1602
Bst4CI ACNGT 3 cut(s) 87, 626, 1211
Bst6I CTCTTC 2 cut(s) 249, 1407
BstAFI CTTAAG 1 cut(s) 500
BstBI TTCGAA 2 cut(s) 252, 1107
BstC8I GCNNGC 4 cut(s) 271, 631, 635, 1189
BstDEI CTNAG 6 cut(s) 161, 177, 573, 809, 1135, 1499
BstDSI CCRYGG 1 cut(s) 1069
BstF5I GGATG 2 cut(s) 768, 1375
BstFNI CGCG 2 cut(s) 149, 999
BstKTI GATC 8 cut(s) 187, 223, 361, 1078, 1297, 1330, 1465, 1588
BstMAI GTCTC 3 cut(s) 953, 1530, 1607
BstMBI GATC 8 cut(s) 184, 220, 358, 1075, 1294, 1327, 1462, 1585
BstMWI GCNNNNNNNGC 6 cut(s) 275, 936, 1005, 1100, 1169, 1370
BstNI CCWGG 2 cut(s) 852, 1602
BstNSI RCATGY 1 cut(s) 1275
BstSCI CCNGG 2 cut(s) 850, 1600
BstSFI CTRYAG 1 cut(s) 909
BstUI CGCG 2 cut(s) 149, 999
BstV1I GCAGC 6 cut(s) 895, 1111, 1159, 1219, 1243, 1348
BstX2I RGATCY 2 cut(s) 1327, 1585
BstYI RGATCY 2 cut(s) 1327, 1585
BstZ17I GTATAC 2 cut(s) 1142, 1379
Bsu36I CCTNAGG 1 cut(s) 1499
BsuI GTATCC 1 cut(s) 825
BsuRI GGCC 2 cut(s) 136, 924
BtgI CCRYGG 1 cut(s) 1069
BtgZI GCGATG 1 cut(s) 1014
BtsCI GGATG 2 cut(s) 768, 1375
BtsI GCAGTG 1 cut(s) 1182
BtsIMutI CAGTG 8 cut(s) 39, 305, 618, 631, 642, 875, 1144, 1182
BtuMI TCGCGA 2 cut(s) 149, 999
BveI ACCTGC 1 cut(s) 1339
Cac8I GCNNGC 4 cut(s) 271, 631, 635, 1189
CaiI CAGNNNCTG 1 cut(s) 1354
Cfr13I GGNCC 3 cut(s) 794, 944, 1223
Csp6I GTAC 2 cut(s) 174, 1048
CspCI CAANNNNNGTGG 4 cut(s) 821, 856, 914, 949
CviQI GTAC 2 cut(s) 174, 1048
DdeI CTNAG 6 cut(s) 161, 177, 573, 809, 1135, 1499
DpnI GATC 8 cut(s) 186, 222, 360, 1077, 1296, 1329, 1464, 1587
DpnII GATC 8 cut(s) 184, 220, 358, 1075, 1294, 1327, 1462, 1585
DraI TTTAAA 1 cut(s) 375
DraIII CACNNNGTG 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 249, 1407
EarI CTCTTC 2 cut(s) 249, 1407
Ecl136II GAGCTC 2 cut(s) 53, 78
Eco130I CCWWGG 1 cut(s) 1069
Eco147I AGGCCT 1 cut(s) 136
Eco24I GRGCYC 2 cut(s) 55, 80
Eco31I GGTCTC 1 cut(s) 1607
Eco47I GGWCC 3 cut(s) 794, 944, 1223
Eco53kI GAGCTC 2 cut(s) 53, 78
Eco81I CCTNAGG 1 cut(s) 1499
Eco88I CYCGRG 1 cut(s) 1622
EcoICRI GAGCTC 2 cut(s) 53, 78
EcoO109I RGGNCCY 1 cut(s) 944
EcoRI GAATTC 2 cut(s) 749, 863
EcoRII CCWGG 2 cut(s) 850, 1600
EcoT14I CCWWGG 1 cut(s) 1069
EcoT22I ATGCAT 1 cut(s) 1569
EcoT38I GRGCYC 2 cut(s) 55, 80
ErhI CCWWGG 1 cut(s) 1069
FaqI GGGAC 2 cut(s) 997, 1618
FbaI TGATCA 2 cut(s) 220, 1294
FblI GTMKAC 2 cut(s) 1141, 1378
Fnu4HI GCNGC 6 cut(s) 909, 1125, 1173, 1233, 1257, 1362
FokI GGATG 2 cut(s) 755, 1382
FriOI GRGCYC 2 cut(s) 55, 80
Fsp4HI GCNGC 6 cut(s) 909, 1125, 1173, 1233, 1257, 1362
FspBI CTAG 6 cut(s) 132, 791, 1164, 1340, 1374, 1389
GluI GCNGC 6 cut(s) 909, 1125, 1173, 1233, 1257, 1362
GsuI CTGGAG 2 cut(s) 475, 1584
HaeIII GGCC 2 cut(s) 136, 924
HapII CCGG 3 cut(s) 126, 344, 1583
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HindIII AAGCTT 2 cut(s) 44, 727
HinfI GANTC 8 cut(s) 16, 212, 428, 484, 505, 1032, 1523, 1551
HpaII CCGG 3 cut(s) 126, 344, 1583
HphI GGTGA 5 cut(s) 166, 592, 872, 1085, 1583
Hpy166II GTNNAC 4 cut(s) 406, 709, 1142, 1379
Hpy188I TCNGA 6 cut(s) 57, 75, 331, 480, 1062, 1518
Hpy8I GTNNAC 4 cut(s) 406, 709, 1142, 1379
Hpy99I CGWCG 3 cut(s) 61, 209, 212
HpyAV CCTTC 5 cut(s) 145, 828, 1103, 1328, 1415
HpyCH4III ACNGT 3 cut(s) 87, 626, 1211
HpyCH4IV ACGT 1 cut(s) 676
HpyCH4V TGCA 8 cut(s) 247, 559, 830, 911, 1119, 1127, 1235, 1567
HpyF10VI GCNNNNNNNGC 6 cut(s) 275, 936, 1005, 1100, 1169, 1370
HpyF3I CTNAG 6 cut(s) 161, 177, 573, 809, 1135, 1499
HpySE526I ACGT 1 cut(s) 676
Kpn2I TCCGGA 1 cut(s) 1582
Ksp22I TGATCA 2 cut(s) 220, 1294
Kzo9I GATC 8 cut(s) 184, 220, 358, 1075, 1294, 1327, 1462, 1585
LguI GCTCTTC 2 cut(s) 249, 1407
LmnI GCTCC 6 cut(s) 50, 58, 75, 349, 805, 1603
Lsp1109I GCAGC 6 cut(s) 895, 1111, 1159, 1219, 1243, 1348
LweI GCATC 2 cut(s) 1243, 1360
MaeI CTAG 6 cut(s) 132, 791, 1164, 1340, 1374, 1389
MaeII ACGT 1 cut(s) 676
MaeIII GTNAC 6 cut(s) 81, 429, 461, 638, 1281, 1424
MalI GATC 8 cut(s) 186, 222, 360, 1077, 1296, 1329, 1464, 1587
MboI GATC 8 cut(s) 184, 220, 358, 1075, 1294, 1327, 1462, 1585
MboII GAAGA 4 cut(s) 194, 248, 266, 1394
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 2 cut(s) 1327, 1585
MhlI GDGCHC 2 cut(s) 55, 80
MlyI GAGTC 2 cut(s) 437, 1532
MmeI TCCRAC 4 cut(s) 76, 80, 526, 766
Mph1103I ATGCAT 1 cut(s) 1569
MroI TCCGGA 1 cut(s) 1582
MroXI GAANNNNTTC 1 cut(s) 1578
MseI TTAA 9 cut(s) 365, 374, 384, 501, 683, 899, 1041, 1242, 1506
MslI CAYNNNNRTG 2 cut(s) 531, 1274
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 3 cut(s) 126, 344, 1583
MspR9I CCNGG 2 cut(s) 852, 1602
MunI CAATTG 1 cut(s) 195
Mva1269I GAATGC 3 cut(s) 653, 1363, 1569
MvaI CCWGG 2 cut(s) 852, 1602
MvnI CGCG 2 cut(s) 149, 999
MwoI GCNNNNNNNGC 6 cut(s) 275, 936, 1005, 1100, 1169, 1370
NcoI CCATGG 1 cut(s) 1069
NdeII GATC 8 cut(s) 184, 220, 358, 1075, 1294, 1327, 1462, 1585
NlaIV GGNNCC 2 cut(s) 342, 1599
NmuCI GTSAC 1 cut(s) 429
NruI TCGCGA 2 cut(s) 149, 999
NsiI ATGCAT 1 cut(s) 1569
NspI RCATGY 1 cut(s) 1275
NspV TTCGAA 2 cut(s) 252, 1107
OliI CACNNNNGTG 2 cut(s) 531, 1274
PaqCI CACCTGC 1 cut(s) 1339
PceI AGGCCT 1 cut(s) 136
PciI ACATGT 1 cut(s) 1271
PciSI GCTCTTC 2 cut(s) 249, 1407
PctI GAATGC 3 cut(s) 653, 1363, 1569
PdmI GAANNNNTTC 1 cut(s) 1578
PfeI GAWTC 6 cut(s) 16, 212, 484, 505, 1032, 1551
PflFI GACNNNGTC 1 cut(s) 792
PkrI GCNGC 6 cut(s) 910, 1126, 1174, 1234, 1258, 1363
PleI GAGTC 2 cut(s) 436, 1531
PpsI GAGTC 2 cut(s) 436, 1531
PpuMI RGGWCCY 1 cut(s) 944
PscI ACATGT 1 cut(s) 1271
PsiI TTATAA 2 cut(s) 450, 966
Psp124BI GAGCTC 2 cut(s) 55, 80
Psp5II RGGWCCY 1 cut(s) 944
Psp6I CCWGG 2 cut(s) 850, 1600
PspGI CCWGG 2 cut(s) 850, 1600
PspN4I GGNNCC 2 cut(s) 342, 1599
PspPI GGNCC 3 cut(s) 794, 944, 1223
PspPPI RGGWCCY 1 cut(s) 944
PstI CTGCAG 1 cut(s) 913
PstNI CAGNNNCTG 1 cut(s) 1354
PsuI RGATCY 2 cut(s) 1327, 1585
PsyI GACNNNGTC 1 cut(s) 792
RruI TCGCGA 2 cut(s) 149, 999
RsaI GTAC 2 cut(s) 175, 1049
RsaNI GTAC 2 cut(s) 174, 1048
RseI CAYNNNNRTG 2 cut(s) 531, 1274
SacI GAGCTC 2 cut(s) 55, 80
SapI GCTCTTC 2 cut(s) 249, 1407
SaqAI TTAA 9 cut(s) 365, 374, 384, 501, 683, 899, 1041, 1242, 1506
SatI GCNGC 6 cut(s) 909, 1125, 1173, 1233, 1257, 1362
Sau3AI GATC 8 cut(s) 184, 220, 358, 1075, 1294, 1327, 1462, 1585
Sau96I GGNCC 3 cut(s) 794, 944, 1223
ScaI AGTACT 1 cut(s) 175
SchI GAGTC 2 cut(s) 437, 1532
ScrFI CCNGG 2 cut(s) 852, 1602
SduI GDGCHC 2 cut(s) 55, 80
SfaNI GCATC 2 cut(s) 1243, 1360
SfcI CTRYAG 1 cut(s) 909
SfuI TTCGAA 2 cut(s) 252, 1107
SinI GGWCC 3 cut(s) 794, 944, 1223
SmiMI CAYNNNNRTG 2 cut(s) 531, 1274
SmlI CTYRAG 3 cut(s) 500, 701, 1051
SmoI CTYRAG 3 cut(s) 500, 701, 1051
SseBI AGGCCT 1 cut(s) 136
SspI AATATT 1 cut(s) 884
SspMI CTAG 6 cut(s) 132, 791, 1164, 1340, 1374, 1389
SstI GAGCTC 2 cut(s) 55, 80
StuI AGGCCT 1 cut(s) 136
StyD4I CCNGG 2 cut(s) 850, 1600
StyI CCWWGG 1 cut(s) 1069
TaaI ACNGT 3 cut(s) 87, 626, 1211
TaiI ACGT 1 cut(s) 679
TaqI TCGA 3 cut(s) 187, 252, 1107
TatI WGTACW 1 cut(s) 173
TfiI GAWTC 6 cut(s) 16, 212, 484, 505, 1032, 1551
Tru1I TTAA 9 cut(s) 365, 374, 384, 501, 683, 899, 1041, 1242, 1506
Tru9I TTAA 9 cut(s) 365, 374, 384, 501, 683, 899, 1041, 1242, 1506
TscAI CASTG 8 cut(s) 39, 312, 625, 631, 649, 875, 1151, 1189
TseFI GTSAC 1 cut(s) 429
TseI GCWGC 6 cut(s) 908, 1124, 1172, 1232, 1256, 1361
Tsp45I GTSAC 1 cut(s) 429
TspDTI ATGAA 6 cut(s) 249, 338, 762, 810, 905, 1473
TspGWI ACGGA 2 cut(s) 706, 1300
TspRI CASTG 8 cut(s) 39, 312, 625, 631, 649, 875, 1151, 1189
Tth111I GACNNNGTC 1 cut(s) 792
Vha464I CTTAAG 1 cut(s) 500
VpaK11BI GGWCC 3 cut(s) 794, 944, 1223
XapI RAATTY 4 cut(s) 740, 749, 863, 1096
XceI RCATGY 1 cut(s) 1275
XmiI GTMKAC 2 cut(s) 1141, 1378
XmnI GAANNNNTTC 1 cut(s) 1578
XspI CTAG 6 cut(s) 132, 791, 1164, 1340, 1374, 1389
ZrmI AGTACT 1 cut(s) 175
Zsp2I ATGCAT 1 cut(s) 1569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.