MD01G1059800.v1.1

Glycosyl hydrolase family 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006678
Physical Location & Seq
Reverse (-)
16365085 .. 16375622
10538 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1059800.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGTTGATTGCTGGTGGGGAATCGTAGAGGCACACAGTCCGCAGGGATATAATTGGGGTGGCTACAAGAAACTATTCCAAATTGTGCGCGATCTTAACCTTAAGTTACAGGTCGTGATGTCATTCCACGAGTGTGGAGGCAATGTCGGGGATGATGTACATATCCCACTTCCTCACTGGGTGACAGAAATTGGCCAAAAGAATCCTGATATATATTTTACTAATAAAGAAGGGAAACGCAACACTGAATGTCTCACATGGGGAATTGATAAAGAGCGGGTTTTAAGAGGCCGCACTGCCGTTGAGGTTTACTTCGACTACATGAGGAGCTTCAGAGTTGAATTTGATGAGTTTTTTGAGGAAGGAATAATATCAGAAATTGAAGTTGGATTAGGTCCGTGTGGAGAGCTACGGTATCCTTCTTATCCTGAACAGCATGGTTGGAAATATCCTGGTATTGGTGAATTCCAGTGTTATGACCGATACTTGATGAAGAATCTGACGCAGGCTGCAGAAGCAAGGGGCCACACCTTCTGGGCCAGAGTACCAGATAATGCAGGTTCTTATAATTCCCAACCACATGAGACAGGGTTTTTCCGTGACGGAGGTGATTATGATAGCTACTATGGCAGATTCTTTCTTAATTGGTACTCCCGTGTTTTGATTGATCATGGTGACCGTGTACTTGCTCTGGCCAATTTAGCTTTTGAAGGCACACGCATTGCTACAAAGGTATCAGGTATCCACTGGTGGTACAAGACTGCCAGCCATCCTGCTGAATTAACTGCTGGATTTTACAACTCCTGCAATCGTGATGGGTATGCGCCAATATCAGCAATGTTAAAAAAGCATGAGGCTGCTCTGAATTTCACATGTGTTGAAATGCGCACATTAGACCAACATGAGGGCTTTCCGGAAGCACTGGCAGACCCTGAGGGATTAGTTTGGCAGGTGCTGAATGCTGCATGGGATGCTAACATTCCAGTTGCTAGTGAGAATGCTCTTACTTGCTTTGACAGAGAAGGCTACAACAAGATATTAGAAAATGCTAAGCCCTGGAACGATCCAGATGGCAGACATTTATCAGCATTTACCTATCTCAGATTAAGCCCAGTTCTCATTGAGAGGCATAACCTAACGGAGTTCGAACGATTTGTCAAGAGAATGCATGGAGAAGCCGCTTCTGATGTTTAG

Protein Analysis

398

Amino Acids

45.64

Weight (kDa)

5.48

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 1 - 380 1.3e-120 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 567
AarI CACCTGC 1 cut(s) 940
Acc16I TGCGCA 1 cut(s) 887
Acc36I ACCTGC 2 cut(s) 548, 940
AccBSI CCGCTC 1 cut(s) 277
AccII CGCG 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 913
AciI CCGC 4 cut(s) 41, 277, 292, 1179
AclWI GGATC 1 cut(s) 1058
AcoI YGGCCR 2 cut(s) 193, 693
AcsI RAATTY 3 cut(s) 341, 464, 865
AcuI CTGAAG 1 cut(s) 316
AdeI CACNNNGTG 1 cut(s) 181
AfaI GTAC 5 cut(s) 159, 546, 650, 684, 755
AfiI CCNNNNNNNGG 2 cut(s) 458, 904
AflII CTTAAG 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 872
AgsI TTSAA 4 cut(s) 341, 383, 710, 881
AjnI CCWGG 2 cut(s) 451, 1055
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AleI CACNNNNGTG 1 cut(s) 132
AluBI AGCT 4 cut(s) 330, 409, 621, 704
AluI AGCT 4 cut(s) 330, 409, 621, 704
Alw26I GTCTC 2 cut(s) 257, 578
AlwI GGATC 1 cut(s) 1058
AlwNI CAGNNNCTG 2 cut(s) 932, 955
Aor13HI TCCGGA 1 cut(s) 913
AoxI GGCC 5 cut(s) 193, 289, 523, 537, 693
ApeKI GCWGC 3 cut(s) 509, 857, 962
ApoI RAATTY 3 cut(s) 341, 464, 865
ArsI GACNNNNNNTTYG 2 cut(s) 1140, 1172
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 3 cut(s) 90, 826, 888
AspS9I GGNCC 3 cut(s) 395, 523, 537
AsuHPI GGTGA 4 cut(s) 193, 473, 620, 686
AsuII TTCGAA 1 cut(s) 1146
AvaII GGWCC 1 cut(s) 395
AxyI CCTNAGG 1 cut(s) 933
BaeI ACNNNNGTAYC 4 cut(s) 640, 673, 717, 750
BalI TGGCCA 2 cut(s) 195, 695
BauI CACGAG 1 cut(s) 128
BbvI GCAGC 3 cut(s) 496, 844, 949
BccI CCATC 3 cut(s) 777, 809, 1064
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 2 cut(s) 453, 1057
BciVI GTATCC 2 cut(s) 426, 752
BclI TGATCA 1 cut(s) 667
BcoDI GTCTC 2 cut(s) 257, 578
BfaI CTAG 1 cut(s) 990
BfmI CTRYAG 1 cut(s) 510
BfrI CTTAAG 1 cut(s) 101
BfuAI ACCTGC 2 cut(s) 548, 940
BfuI GTATCC 2 cut(s) 426, 752
BisI GCNGC 5 cut(s) 292, 510, 858, 963, 1179
BlpI GCTNAGC 1 cut(s) 1050
BlsI GCNGC 5 cut(s) 293, 511, 859, 964, 1180
Bme1390I CCNGG 2 cut(s) 453, 1057
Bme18I GGWCC 1 cut(s) 395
BmgT120I GGNCC 3 cut(s) 395, 523, 537
BmiI GGNNCC 1 cut(s) 524
BmrFI CCNGG 2 cut(s) 453, 1057
BmrI ACTGGG 2 cut(s) 187, 1106
BmsI GCATC 1 cut(s) 961
BmuI ACTGGG 2 cut(s) 187, 1106
Bpu1102I GCTNAGC 1 cut(s) 1050
Bpu14I TTCGAA 1 cut(s) 1146
BsaJI CCNNGG 1 cut(s) 1055
BsaWI WCCGGW 1 cut(s) 913
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 904
Bse1I ACTGG 6 cut(s) 182, 469, 752, 927, 983, 1112
Bse21I CCTNAGG 1 cut(s) 933
Bse3DI GCAATG 3 cut(s) 148, 720, 843
BseAI TCCGGA 1 cut(s) 913
BseBI CCWGG 2 cut(s) 453, 1057
BseDI CCNNGG 1 cut(s) 1055
BseGI GGATG 3 cut(s) 157, 769, 976
BseLI CCNNNNNNNGG 2 cut(s) 458, 904
BseMI GCAATG 3 cut(s) 148, 720, 843
BseMII CTCAG 2 cut(s) 924, 1114
BseNI ACTGG 6 cut(s) 182, 469, 752, 927, 983, 1112
BseRI GAGGAG 1 cut(s) 340
BseXI GCAGC 3 cut(s) 496, 844, 949
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 5 cut(s) 195, 291, 525, 539, 695
BsiSI CCGG 1 cut(s) 914
BslI CCNNNNNNNGG 2 cut(s) 458, 904
BsmAI GTCTC 2 cut(s) 257, 578
BsmI GAATGC 3 cut(s) 964, 1003, 1170
BsnI GGCC 5 cut(s) 195, 291, 525, 539, 695
Bsp119I TTCGAA 1 cut(s) 1146
Bsp13I TCCGGA 1 cut(s) 913
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 3 cut(s) 91, 667, 1063
Bsp1720I GCTNAGC 1 cut(s) 1050
BspACI CCGC 4 cut(s) 41, 277, 292, 1179
BspANI GGCC 5 cut(s) 195, 291, 525, 539, 695
BspCNI CTCAG 2 cut(s) 925, 1113
BspEI TCCGGA 1 cut(s) 913
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 524
BspMAI CTGCAG 1 cut(s) 514
BspMI ACCTGC 2 cut(s) 548, 940
BspPI GGATC 1 cut(s) 1058
BspT104I TTCGAA 1 cut(s) 1146
BspTI CTTAAG 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 277
BsrDI GCAATG 3 cut(s) 148, 720, 843
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 6 cut(s) 182, 469, 752, 927, 983, 1112
BssECI CCNNGG 1 cut(s) 1055
BssMI GATC 3 cut(s) 91, 667, 1063
BssSI CACGAG 1 cut(s) 128
Bst2BI CACGAG 1 cut(s) 128
Bst2UI CCWGG 2 cut(s) 453, 1057
Bst4CI ACNGT 3 cut(s) 38, 414, 680
BstAFI CTTAAG 1 cut(s) 101
BstAPI GCANNNNNTGC 1 cut(s) 971
BstAUI TGTACA 1 cut(s) 157
BstBI TTCGAA 1 cut(s) 1146
BstC8I GCNNGC 2 cut(s) 507, 766
BstDEI CTNAG 3 cut(s) 933, 1050, 1100
BstEII GGTNACC 1 cut(s) 674
BstF5I GGATG 3 cut(s) 157, 769, 976
BstFNI CGCG 1 cut(s) 90
BstHHI GCGC 3 cut(s) 90, 826, 888
BstKTI GATC 3 cut(s) 94, 670, 1066
BstMAI GTCTC 2 cut(s) 257, 578
BstMBI GATC 3 cut(s) 91, 667, 1063
BstMWI GCNNNNNNNGC 4 cut(s) 515, 627, 701, 971
BstNI CCWGG 2 cut(s) 453, 1057
BstNSI RCATGY 1 cut(s) 876
BstPI GGTNACC 1 cut(s) 674
BstSCI CCNGG 2 cut(s) 451, 1055
BstSFI CTRYAG 1 cut(s) 510
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 496, 844, 949
BstXI CCANNNNNNTGG 1 cut(s) 134
Bsu36I CCTNAGG 1 cut(s) 933
BsuI GTATCC 2 cut(s) 426, 752
BsuRI GGCC 5 cut(s) 195, 291, 525, 539, 695
BtsCI GGATG 3 cut(s) 157, 769, 976
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 6 cut(s) 175, 243, 294, 476, 745, 920
BveI ACCTGC 2 cut(s) 548, 940
Cac8I GCNNGC 2 cut(s) 507, 766
CaiI CAGNNNCTG 2 cut(s) 932, 955
CfoI GCGC 3 cut(s) 90, 826, 888
Cfr13I GGNCC 3 cut(s) 395, 523, 537
CseI GACGC 1 cut(s) 511
Csp6I GTAC 5 cut(s) 158, 545, 649, 683, 754
CviQI GTAC 5 cut(s) 158, 545, 649, 683, 754
DdeI CTNAG 3 cut(s) 933, 1050, 1100
DpnI GATC 3 cut(s) 93, 669, 1065
DpnII GATC 3 cut(s) 91, 667, 1063
DraIII CACNNNGTG 1 cut(s) 181
EaeI YGGCCR 2 cut(s) 193, 693
Eco47I GGWCC 1 cut(s) 395
Eco57I CTGAAG 1 cut(s) 316
Eco81I CCTNAGG 1 cut(s) 933
Eco91I GGTNACC 1 cut(s) 674
EcoO65I GGTNACC 1 cut(s) 674
EcoRI GAATTC 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 451, 1055
EcoT22I ATGCAT 1 cut(s) 1170
FauI CCCGC 1 cut(s) 270
FbaI TGATCA 1 cut(s) 667
Fnu4HI GCNGC 5 cut(s) 292, 510, 858, 963, 1179
FokI GGATG 3 cut(s) 164, 756, 983
Fsp4HI GCNGC 5 cut(s) 292, 510, 858, 963, 1179
FspAI RTGCGCAY 1 cut(s) 887
FspBI CTAG 1 cut(s) 990
FspI TGCGCA 1 cut(s) 887
GlaI GCGC 3 cut(s) 89, 825, 887
GluI GCNGC 5 cut(s) 292, 510, 858, 963, 1179
HaeIII GGCC 5 cut(s) 195, 291, 525, 539, 695
HapII CCGG 1 cut(s) 914
HgaI GACGC 1 cut(s) 511
HhaI GCGC 3 cut(s) 90, 826, 888
Hin6I GCGC 3 cut(s) 88, 824, 886
HinP1I GCGC 3 cut(s) 88, 824, 886
HinfI GANTC 4 cut(s) 21, 202, 496, 633
HpaII CCGG 1 cut(s) 914
HphI GGTGA 4 cut(s) 193, 473, 620, 686
Hpy166II GTNNAC 2 cut(s) 310, 683
Hpy188I TCNGA 6 cut(s) 335, 376, 501, 864, 1103, 1186
Hpy188III TCNNGA 7 cut(s) 115, 206, 428, 812, 914, 1067, 1159
Hpy8I GTNNAC 2 cut(s) 310, 683
HpyAV CCTTC 6 cut(s) 224, 356, 429, 541, 704, 1016
HpyCH4III ACNGT 3 cut(s) 38, 414, 680
HpyCH4V TGCA 5 cut(s) 512, 557, 807, 965, 1168
HpyF10VI GCNNNNNNNGC 4 cut(s) 515, 627, 701, 971
HpyF3I CTNAG 3 cut(s) 933, 1050, 1100
HspAI GCGC 3 cut(s) 88, 824, 886
Kpn2I TCCGGA 1 cut(s) 913
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 3 cut(s) 91, 667, 1063
LmnI GCTCC 1 cut(s) 327
Lsp1109I GCAGC 3 cut(s) 496, 844, 949
LweI GCATC 1 cut(s) 961
MaeI CTAG 1 cut(s) 990
MaeIII GTNAC 4 cut(s) 105, 181, 599, 674
MalI GATC 3 cut(s) 93, 669, 1065
MbiI CCGCTC 1 cut(s) 277
MboI GATC 3 cut(s) 91, 667, 1063
MboII GAAGA 1 cut(s) 505
MlsI TGGCCA 2 cut(s) 195, 695
MluNI TGGCCA 2 cut(s) 195, 695
MmeI TCCRAC 2 cut(s) 367, 422
Mox20I TGGCCA 2 cut(s) 195, 695
Mph1103I ATGCAT 1 cut(s) 1170
MroI TCCGGA 1 cut(s) 913
MroXI GAANNNNTTC 1 cut(s) 74
MscI TGGCCA 2 cut(s) 195, 695
MseI TTAA 7 cut(s) 96, 102, 284, 642, 782, 842, 1106
MslI CAYNNNNRTG 1 cut(s) 132
Msp20I TGGCCA 2 cut(s) 195, 695
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 1 cut(s) 914
MspR9I CCNGG 2 cut(s) 453, 1057
Mva1269I GAATGC 3 cut(s) 964, 1003, 1170
MvaI CCWGG 2 cut(s) 453, 1057
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 4 cut(s) 515, 627, 701, 971
NdeII GATC 3 cut(s) 91, 667, 1063
NlaIV GGNNCC 1 cut(s) 524
NmuCI GTSAC 3 cut(s) 181, 599, 674
NsbI TGCGCA 1 cut(s) 887
NsiI ATGCAT 1 cut(s) 1170
NspI RCATGY 1 cut(s) 876
NspV TTCGAA 1 cut(s) 1146
OliI CACNNNNGTG 1 cut(s) 132
PaqCI CACCTGC 1 cut(s) 940
PciI ACATGT 1 cut(s) 872
PctI GAATGC 3 cut(s) 964, 1003, 1170
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 4 cut(s) 21, 202, 496, 633
PkrI GCNGC 5 cut(s) 293, 511, 859, 964, 1180
PscI ACATGT 1 cut(s) 872
PsiI TTATAA 1 cut(s) 567
Psp6I CCWGG 2 cut(s) 451, 1055
PspEI GGTNACC 1 cut(s) 674
PspGI CCWGG 2 cut(s) 451, 1055
PspN4I GGNNCC 1 cut(s) 524
PspPI GGNCC 3 cut(s) 395, 523, 537
PstI CTGCAG 1 cut(s) 514
PstNI CAGNNNCTG 2 cut(s) 932, 955
RsaI GTAC 5 cut(s) 159, 546, 650, 684, 755
RsaNI GTAC 5 cut(s) 158, 545, 649, 683, 754
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 7 cut(s) 96, 102, 284, 642, 782, 842, 1106
SatI GCNGC 5 cut(s) 292, 510, 858, 963, 1179
Sau3AI GATC 3 cut(s) 91, 667, 1063
Sau96I GGNCC 3 cut(s) 395, 523, 537
ScrFI CCNGG 2 cut(s) 453, 1057
SfaNI GCATC 1 cut(s) 961
SfcI CTRYAG 1 cut(s) 510
SfuI TTCGAA 1 cut(s) 1146
SinI GGWCC 1 cut(s) 395
SmiMI CAYNNNNRTG 1 cut(s) 132
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SsiI CCGC 4 cut(s) 41, 277, 292, 1179
SspMI CTAG 1 cut(s) 990
StyD4I CCNGG 2 cut(s) 451, 1055
TaaI ACNGT 3 cut(s) 38, 414, 680
TaqI TCGA 2 cut(s) 315, 1146
TaqII GACCGA 1 cut(s) 495
TatI WGTACW 2 cut(s) 157, 682
TauI GCSGC 2 cut(s) 294, 1181
TfiI GAWTC 4 cut(s) 21, 202, 496, 633
Tru1I TTAA 7 cut(s) 96, 102, 284, 642, 782, 842, 1106
Tru9I TTAA 7 cut(s) 96, 102, 284, 642, 782, 842, 1106
TscAI CASTG 6 cut(s) 182, 250, 301, 476, 752, 927
TseFI GTSAC 3 cut(s) 181, 599, 674
TseI GCWGC 3 cut(s) 509, 857, 962
Tsp45I GTSAC 3 cut(s) 181, 599, 674
TspDTI ATGAA 1 cut(s) 506
TspGWI ACGGA 4 cut(s) 387, 587, 618, 1154
TspRI CASTG 6 cut(s) 182, 250, 301, 476, 752, 927
Vha464I CTTAAG 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 3 cut(s) 341, 464, 865
XceI RCATGY 1 cut(s) 876
XcmI CCANNNNNNNNNTGG 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 1 cut(s) 990
Zsp2I ATGCAT 1 cut(s) 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.