FvH4_7g14220

Glycosyl hydrolase family 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006678
Physical Location & Seq
Reverse (-)
12578259 .. 12582739
4481 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g14220.t1

Sequence Viewer

Length: 1635 bp
ATGGCTCTACTCTCACTTCAACCTTTCCGTTGCTTCACTCCACGGAGACCAAGTAACGTTTCTAAAACGACAATGCCTGCGTTGTCGTTCTCTCCGGGGCTTTGGGGATCGCCGGAGTTTGACTCGAGGAGGTTGAGGGTGGTGGGTGGTGGGAAGTATAGGAGGTTGGCGGTGGTGAGTGCGGAGGGGAGTGTGGTGGAGAATGTTGGTGATGAGCAGGTTGCAGAGGTGGCACAAATTGTTCAGGAGCGTGATTTCACTGGGACTCCTTATGTTCCTATATATGTGATGTTACCGTTGGGTGTCATCAATATGAACAGTGAGGTAGTTGAGCCAGAAGTTCTGATGGATCAACTGCGAGCGTTGAAGTCAGTTGGTGTTGACGGTGTTATGGTTGATTGCTGGTGGGGGATAGTCGAGGCGCATAATCCACAGTCGTATAATTGGAGTGGCTACAAGAAGCTCTTCCAGATTGTGCGTGATCTGAATCTTAAGTTACAGGTTGTAATGTCATTTCACGAATGCGGAGGCAATGTTGGGGATGATGTTCATATCCCACTTCCTTACTGGGTGACAGAAATTGGTCAAAAAAATCCTGATATATATTTTACCAATAGAGAAGGGCAACGGATTGTGGAATGCCTCACGTGGGGAATTGATAAGGAGCGTGTCTTAAAAGGCCGGACTGCTGTTGAGGTTTACTTCGATTACATGAGGAGCTTCAGGGTCGAATTTGATGAATTTTTTGCGGAAGGAATTATCTCAGAGATTGAAGTTGGTTTAGGTCCTTGTGGAGAGTTACGGTATCCTTCTTATCCAGCACAGCATGGTTGGAAATATCCTGGAATTGGTGAATTTCAGTGTTATGATCGGTACTTAATGGAGAATCTAAGGAAGGCTGCAGAAGTAAGGGGGCACTCTTTCTGGGCCAGAGCACCCGATAATGCAGGTTCTTACAATTTTCGACCACAAGACACAGGGTTTTTCCGCGATGGAGGTGATTATGACAGCTACTATGGGAGATTCTTCCTTAATTGGTATTCCAATGTTTTGGTGGATCATGGTGATCGTGTACTTACACTGGCCAACTTAGCTTTTGAAGGCACTTCAATTGCTGCAAAGCTATCAGGTATTCATTGGTGGTACAAGACTGCCAGCCATGCTGCTGAATTAACTGCTGGATTTTACAATCCCTGCAATCGTGATGGGTATACTCCAATTGCAGTGATGTTTAAAAAGCATGCGGCTGCCTTGAATTTCACGTGTGTTGAATTGCGCACATTAAACCAGCACGAGGACTTCCCAGAAGCAATGGCTGACCCTGAGGGTTTAGTTTGGCAGGTGCTGAATGCTGCATGGGATGCCAACATTCCAGTTGCTAGTGAGAATGCTCTTAATTGCCATGATAGAGAAGGCTACAACAAGATATTGGAAAATGCCAAACCCCGGAATGATCCAGATGGTAGGCATTTGTCGGCATTTACCTACCTCAGGTTAAGCCCGGTTCTCCTGGAGAGGCATAACTTCATGGAGTTTGAAAGATTTGTCAAGAAAATGCATGGAGAAGCTGCTACAGATCTCCAGATTAATCCAAATGAAGAGAACACTGCAGACTCATCTGCAAATCTGCAGTAG

Protein Analysis

545

Amino Acids

61.75

Weight (kDa)

5.36

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 95 - 508 6e-130 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1330
Acc16I TGCGCA 1 cut(s) 1277
Acc36I ACCTGC 3 cut(s) 208, 938, 1330
AccI GTMKAC 1 cut(s) 1211
AccII CGCG 1 cut(s) 990
AciI CCGC 6 cut(s) 170, 182, 525, 749, 988, 1244
AclI AACGTT 1 cut(s) 57
AclWI GGATC 4 cut(s) 115, 357, 1065, 1448
AcoI YGGCCR 1 cut(s) 1083
AcsI RAATTY 4 cut(s) 731, 740, 854, 1255
AcuI CTGAAG 1 cut(s) 706
AcvI CACGTG 2 cut(s) 648, 1263
AfaI GTAC 3 cut(s) 875, 1074, 1145
AfiI CCNNNNNNNGG 5 cut(s) 649, 848, 1294, 1446, 1491
AflII CTTAAG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 1262
AgsI TTSAA 8 cut(s) 20, 367, 773, 1100, 1110, 1255, 1271, 1538
AjnI CCWGG 2 cut(s) 841, 1509
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 6 cut(s) 463, 720, 1011, 1094, 1123, 1568
AluI AGCT 6 cut(s) 463, 720, 1011, 1094, 1123, 1568
Alw21I GWGCWC 1 cut(s) 937
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 4 cut(s) 115, 357, 1065, 1448
AlwNI CAGNNNCTG 1 cut(s) 1345
Ama87I CYCGRG 1 cut(s) 124
AoxI GGCC 3 cut(s) 679, 927, 1083
ApeKI GCWGC 6 cut(s) 899, 1115, 1163, 1247, 1352, 1568
ApoI RAATTY 4 cut(s) 731, 740, 854, 1255
AseI ATTAAT 1 cut(s) 1587
Asp700I GAANNNNTTC 1 cut(s) 464
AspLEI GCGC 2 cut(s) 424, 1278
AspS9I GGNCC 2 cut(s) 785, 927
AsuC2I CCSGG 3 cut(s) 96, 1447, 1502
AsuHPI GGTGA 6 cut(s) 187, 221, 583, 863, 1010, 1076
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 1 cut(s) 785
AxyI CCTNAGG 2 cut(s) 1323, 1490
BaeGI GKGCMC 1 cut(s) 918
BalI TGGCCA 1 cut(s) 1085
BauI CACGAG 1 cut(s) 1292
BbrPI CACGTG 2 cut(s) 648, 1263
Bbv12I GWGCWC 1 cut(s) 937
BbvI GCAGC 6 cut(s) 886, 1102, 1150, 1234, 1339, 1555
BccI CCATC 4 cut(s) 340, 986, 1199, 1454
BciT130I CCWGG 2 cut(s) 843, 1511
BciVI GTATCC 1 cut(s) 816
BcnI CCSGG 3 cut(s) 96, 1447, 1502
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 1380
BfmI CTRYAG 4 cut(s) 900, 1572, 1608, 1628
BfrI CTTAAG 1 cut(s) 491
BfuAI ACCTGC 3 cut(s) 208, 938, 1330
BfuI GTATCC 1 cut(s) 816
BglII AGATCT 1 cut(s) 1576
BisI GCNGC 7 cut(s) 900, 1116, 1164, 1245, 1248, 1353, 1569
BlsI GCNGC 7 cut(s) 901, 1117, 1165, 1246, 1249, 1354, 1570
Bme1390I CCNGG 5 cut(s) 96, 843, 1447, 1502, 1511
Bme18I GGWCC 1 cut(s) 785
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 2 cut(s) 785, 927
BmrFI CCNGG 5 cut(s) 96, 843, 1447, 1502, 1511
BmrI ACTGGG 2 cut(s) 270, 577
BmsI GCATC 1 cut(s) 1351
BmuI ACTGGG 2 cut(s) 270, 577
BplI GAGNNNNNCTC 2 cut(s) 107, 139
BpmI CTGGAG 2 cut(s) 1532, 1565
BpuMI CCSGG 3 cut(s) 96, 1447, 1502
BsaAI YACGTR 2 cut(s) 648, 1263
BsaBI GATNNNNATC 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 40
BsaJI CCNNGG 3 cut(s) 41, 95, 1445
BsaXI ACNNNNNCTCC 6 cut(s) 37, 67, 250, 280, 519, 549
Bsc4I CCNNNNNNNGG 5 cut(s) 649, 848, 1294, 1446, 1491
Bse1I ACTGG 4 cut(s) 265, 572, 1086, 1373
Bse21I CCTNAGG 2 cut(s) 1323, 1490
Bse3DI GCAATG 2 cut(s) 538, 1317
Bse8I GATNNNNATC 1 cut(s) 486
BseBI CCWGG 2 cut(s) 843, 1511
BseDI CCNNGG 3 cut(s) 41, 95, 1445
BseGI GGATG 2 cut(s) 547, 1366
BseJI GATNNNNATC 1 cut(s) 486
BseLI CCNNNNNNNGG 5 cut(s) 649, 848, 1294, 1446, 1491
BseMI GCAATG 2 cut(s) 538, 1317
BseMII CTCAG 3 cut(s) 777, 1314, 1504
BseNI ACTGG 4 cut(s) 265, 572, 1086, 1373
BseRI GAGGAG 2 cut(s) 142, 730
BseSI GKGCMC 1 cut(s) 918
BseXI GCAGC 6 cut(s) 886, 1102, 1150, 1234, 1339, 1555
Bsh1236I CGCG 1 cut(s) 990
BshFI GGCC 3 cut(s) 681, 929, 1085
BsiHKAI GWGCWC 1 cut(s) 937
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 5 cut(s) 95, 113, 682, 1447, 1502
BslFI GGGAC 1 cut(s) 277
BslI CCNNNNNNNGG 5 cut(s) 649, 848, 1294, 1446, 1491
BsmAI GTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 277
BsmI GAATGC 4 cut(s) 527, 644, 1354, 1393
BsnI GGCC 3 cut(s) 681, 929, 1085
Bso31I GGTCTC 1 cut(s) 40
BsoBI CYCGRG 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 918, 937
Bsp143I GATC 8 cut(s) 107, 349, 481, 868, 1057, 1066, 1453, 1576
BspACI CCGC 6 cut(s) 170, 182, 525, 749, 988, 1244
BspANI GGCC 3 cut(s) 681, 929, 1085
BspCNI CTCAG 3 cut(s) 776, 1315, 1503
BspFNI CGCG 1 cut(s) 990
BspMAI CTGCAG 3 cut(s) 904, 1612, 1632
BspMI ACCTGC 3 cut(s) 208, 938, 1330
BspPI GGATC 4 cut(s) 115, 357, 1065, 1448
BspQI GCTCTTC 1 cut(s) 470
BspTI CTTAAG 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 40
BsrDI GCAATG 2 cut(s) 538, 1317
BsrI ACTGG 4 cut(s) 265, 572, 1086, 1373
BssECI CCNNGG 3 cut(s) 41, 95, 1445
BssMI GATC 8 cut(s) 107, 349, 481, 868, 1057, 1066, 1453, 1576
BssNAI GTATAC 1 cut(s) 1212
BssSI CACGAG 1 cut(s) 1292
Bst1107I GTATAC 1 cut(s) 1212
Bst2BI CACGAG 1 cut(s) 1292
Bst2UI CCWGG 2 cut(s) 843, 1511
Bst4CI ACNGT 5 cut(s) 297, 320, 386, 435, 804
Bst6I CTCTTC 2 cut(s) 470, 1593
BstAFI CTTAAG 1 cut(s) 491
BstAPI GCANNNNNTGC 1 cut(s) 1361
BstBAI YACGTR 2 cut(s) 648, 1263
BstC8I GCNNGC 4 cut(s) 78, 360, 1156, 1242
BstDEI CTNAG 5 cut(s) 763, 890, 1090, 1323, 1490
BstDSI CCRYGG 1 cut(s) 41
BstENI CCTNNNNNAGG 1 cut(s) 1489
BstF5I GGATG 2 cut(s) 547, 1366
BstFNI CGCG 1 cut(s) 990
BstHHI GCGC 2 cut(s) 424, 1278
BstKTI GATC 8 cut(s) 110, 352, 484, 871, 1060, 1069, 1456, 1579
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 8 cut(s) 107, 349, 481, 868, 1057, 1066, 1453, 1576
BstMWI GCNNNNNNNGC 4 cut(s) 230, 1091, 1160, 1361
BstNI CCWGG 2 cut(s) 843, 1511
BstNSI RCATGY 1 cut(s) 1244
BstSCI CCNGG 5 cut(s) 94, 841, 1445, 1500, 1509
BstSFI CTRYAG 4 cut(s) 900, 1572, 1608, 1628
BstSLI GKGCMC 1 cut(s) 918
BstUI CGCG 1 cut(s) 990
BstV1I GCAGC 6 cut(s) 886, 1102, 1150, 1234, 1339, 1555
BstX2I RGATCY 1 cut(s) 1576
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 1576
BstZ17I GTATAC 1 cut(s) 1212
Bsu36I CCTNAGG 2 cut(s) 1323, 1490
BsuI GTATCC 1 cut(s) 816
BsuRI GGCC 3 cut(s) 681, 929, 1085
BtgI CCRYGG 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 1005
BtsCI GGATG 2 cut(s) 547, 1366
BtsI GCAGTG 2 cut(s) 1230, 1605
BtsIMutI CAGTG 6 cut(s) 258, 325, 866, 1079, 1230, 1605
BveI ACCTGC 3 cut(s) 208, 938, 1330
Cac8I GCNNGC 4 cut(s) 78, 360, 1156, 1242
CaiI CAGNNNCTG 1 cut(s) 1345
CfoI GCGC 2 cut(s) 424, 1278
Cfr13I GGNCC 2 cut(s) 785, 927
Csp6I GTAC 3 cut(s) 874, 1073, 1144
CviAII CATG 9 cut(s) 712, 827, 1061, 1160, 1241, 1356, 1403, 1528, 1559
CviQI GTAC 3 cut(s) 874, 1073, 1144
DdeI CTNAG 5 cut(s) 763, 890, 1090, 1323, 1490
DpnI GATC 8 cut(s) 109, 351, 483, 870, 1059, 1068, 1455, 1578
DpnII GATC 8 cut(s) 107, 349, 481, 868, 1057, 1066, 1453, 1576
DraI TTTAAA 1 cut(s) 1234
EaeI YGGCCR 1 cut(s) 1083
Eam1104I CTCTTC 2 cut(s) 470, 1593
EarI CTCTTC 2 cut(s) 470, 1593
Eco31I GGTCTC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 785
Eco57I CTGAAG 1 cut(s) 706
Eco72I CACGTG 2 cut(s) 648, 1263
Eco81I CCTNAGG 2 cut(s) 1323, 1490
Eco88I CYCGRG 1 cut(s) 124
EcoNI CCTNNNNNAGG 1 cut(s) 1489
EcoO109I RGGNCCY 1 cut(s) 785
EcoRII CCWGG 2 cut(s) 841, 1509
EcoT22I ATGCAT 1 cut(s) 1560
FaeI CATG 9 cut(s) 715, 830, 1064, 1163, 1244, 1359, 1406, 1531, 1562
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FaqI GGGAC 1 cut(s) 277
FatI CATG 9 cut(s) 711, 826, 1060, 1159, 1240, 1355, 1402, 1527, 1558
FblI GTMKAC 1 cut(s) 1211
Fnu4HI GCNGC 7 cut(s) 900, 1116, 1164, 1245, 1248, 1353, 1569
FokI GGATG 2 cut(s) 554, 1373
Fsp4HI GCNGC 7 cut(s) 900, 1116, 1164, 1245, 1248, 1353, 1569
FspBI CTAG 1 cut(s) 1380
FspI TGCGCA 1 cut(s) 1277
GlaI GCGC 2 cut(s) 423, 1277
GluI GCNGC 7 cut(s) 900, 1116, 1164, 1245, 1248, 1353, 1569
GsuI CTGGAG 2 cut(s) 1532, 1565
HaeIII GGCC 3 cut(s) 681, 929, 1085
HapII CCGG 5 cut(s) 95, 113, 682, 1447, 1502
HhaI GCGC 2 cut(s) 424, 1278
Hin1II CATG 9 cut(s) 715, 830, 1064, 1163, 1244, 1359, 1406, 1531, 1562
Hin6I GCGC 2 cut(s) 422, 1276
HinP1I GCGC 2 cut(s) 422, 1276
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 6 cut(s) 122, 265, 487, 886, 1023, 1613
HpaII CCGG 5 cut(s) 95, 113, 682, 1447, 1502
HphI GGTGA 6 cut(s) 187, 221, 583, 863, 1010, 1076
Hpy166II GTNNAC 4 cut(s) 382, 700, 1073, 1212
Hpy188I TCNGA 3 cut(s) 345, 486, 766
Hpy188III TCNNGA 8 cut(s) 245, 469, 518, 596, 1202, 1457, 1549, 1582
Hpy8I GTNNAC 4 cut(s) 382, 700, 1073, 1212
HpyAV CCTTC 6 cut(s) 614, 746, 819, 889, 1094, 1406
HpyCH4III ACNGT 5 cut(s) 297, 320, 386, 435, 804
HpyCH4IV ACGT 3 cut(s) 57, 647, 1262
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 1091, 1160, 1361
HpyF3I CTNAG 5 cut(s) 763, 890, 1090, 1323, 1490
HpySE526I ACGT 3 cut(s) 57, 647, 1262
Hsp92II CATG 9 cut(s) 715, 830, 1064, 1163, 1244, 1359, 1406, 1531, 1562
HspAI GCGC 2 cut(s) 422, 1276
Kzo9I GATC 8 cut(s) 107, 349, 481, 868, 1057, 1066, 1453, 1576
LguI GCTCTTC 1 cut(s) 470
LmnI GCTCC 3 cut(s) 247, 664, 717
Lsp1109I GCAGC 6 cut(s) 886, 1102, 1150, 1234, 1339, 1555
LweI GCATC 1 cut(s) 1351
MaeI CTAG 1 cut(s) 1380
MaeII ACGT 3 cut(s) 57, 647, 1262
MaeIII GTNAC 5 cut(s) 53, 291, 495, 571, 798
MalI GATC 8 cut(s) 109, 351, 483, 870, 1059, 1068, 1455, 1578
MboI GATC 8 cut(s) 107, 349, 481, 868, 1057, 1066, 1453, 1576
MboII GAAGA 3 cut(s) 457, 1018, 1610
MfeI CAATTG 2 cut(s) 1110, 1218
MflI RGATCY 1 cut(s) 1576
MhlI GDGCHC 2 cut(s) 918, 937
MlsI TGGCCA 1 cut(s) 1085
MluNI TGGCCA 1 cut(s) 1085
MlyI GAGTC 3 cut(s) 116, 259, 1607
MmeI TCCRAC 1 cut(s) 812
Mox20I TGGCCA 1 cut(s) 1085
Mph1103I ATGCAT 1 cut(s) 1560
MroXI GAANNNNTTC 1 cut(s) 464
MscI TGGCCA 1 cut(s) 1085
MslI CAYNNNNRTG 1 cut(s) 311
Msp20I TGGCCA 1 cut(s) 1085
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 5 cut(s) 95, 113, 682, 1447, 1502
MspR9I CCNGG 5 cut(s) 96, 843, 1447, 1502, 1511
MunI CAATTG 2 cut(s) 1110, 1218
Mva1269I GAATGC 4 cut(s) 527, 644, 1354, 1393
MvaI CCWGG 2 cut(s) 843, 1511
MvnI CGCG 1 cut(s) 990
MwoI GCNNNNNNNGC 4 cut(s) 230, 1091, 1160, 1361
NciI CCSGG 3 cut(s) 96, 1447, 1502
NdeII GATC 8 cut(s) 107, 349, 481, 868, 1057, 1066, 1453, 1576
NlaIII CATG 9 cut(s) 715, 830, 1064, 1163, 1244, 1359, 1406, 1531, 1562
NmuCI GTSAC 1 cut(s) 571
NsbI TGCGCA 1 cut(s) 1277
NsiI ATGCAT 1 cut(s) 1560
NspI RCATGY 1 cut(s) 1244
PaeI GCATGC 1 cut(s) 1244
PaeR7I CTCGAG 1 cut(s) 124
PaqCI CACCTGC 1 cut(s) 1330
PciSI GCTCTTC 1 cut(s) 470
PctI GAATGC 4 cut(s) 527, 644, 1354, 1393
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 3 cut(s) 487, 886, 1023
PfoI TCCNGGA 2 cut(s) 841, 1509
PkrI GCNGC 7 cut(s) 901, 1117, 1165, 1246, 1249, 1354, 1570
PleI GAGTC 3 cut(s) 116, 259, 1607
PmaCI CACGTG 2 cut(s) 648, 1263
PmlI CACGTG 2 cut(s) 648, 1263
PpsI GAGTC 3 cut(s) 116, 259, 1607
Ppu21I YACGTR 2 cut(s) 648, 1263
PpuMI RGGWCCY 1 cut(s) 785
PshBI ATTAAT 1 cut(s) 1587
Psp1406I AACGTT 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 785
Psp6I CCWGG 2 cut(s) 841, 1509
PspCI CACGTG 2 cut(s) 648, 1263
PspGI CCWGG 2 cut(s) 841, 1509
PspPI GGNCC 2 cut(s) 785, 927
PspPPI RGGWCCY 1 cut(s) 785
PspXI VCTCGAGB 1 cut(s) 124
PstI CTGCAG 3 cut(s) 904, 1612, 1632
PstNI CAGNNNCTG 1 cut(s) 1345
PsuI RGATCY 1 cut(s) 1576
RsaI GTAC 3 cut(s) 875, 1074, 1145
RsaNI GTAC 3 cut(s) 874, 1073, 1144
RseI CAYNNNNRTG 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 470
SatI GCNGC 7 cut(s) 900, 1116, 1164, 1245, 1248, 1353, 1569
Sau3AI GATC 8 cut(s) 107, 349, 481, 868, 1057, 1066, 1453, 1576
Sau96I GGNCC 2 cut(s) 785, 927
SchI GAGTC 3 cut(s) 116, 259, 1607
ScrFI CCNGG 5 cut(s) 96, 843, 1447, 1502, 1511
SduI GDGCHC 2 cut(s) 918, 937
SfaNI GCATC 1 cut(s) 1351
SfcI CTRYAG 4 cut(s) 900, 1572, 1608, 1628
Sfr274I CTCGAG 1 cut(s) 124
SinI GGWCC 1 cut(s) 785
SlaI CTCGAG 1 cut(s) 124
SmiMI CAYNNNNRTG 1 cut(s) 311
SmlI CTYRAG 2 cut(s) 124, 491
SmoI CTYRAG 2 cut(s) 124, 491
SphI GCATGC 1 cut(s) 1244
SsiI CCGC 6 cut(s) 170, 182, 525, 749, 988, 1244
SspMI CTAG 1 cut(s) 1380
StyD4I CCNGG 5 cut(s) 94, 841, 1445, 1500, 1509
TaaI ACNGT 5 cut(s) 297, 320, 386, 435, 804
TaiI ACGT 3 cut(s) 60, 650, 1265
TaqI TCGA 5 cut(s) 125, 417, 705, 729, 964
TatI WGTACW 1 cut(s) 1072
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 3 cut(s) 487, 886, 1023
TscAI CASTG 6 cut(s) 265, 325, 866, 1086, 1230, 1612
TseFI GTSAC 1 cut(s) 571
TseI GCWGC 6 cut(s) 899, 1115, 1163, 1247, 1352, 1568
Tsp45I GTSAC 1 cut(s) 571
TspDTI ATGAA 6 cut(s) 329, 539, 753, 1124, 1516, 1611
TspGWI ACGGA 3 cut(s) 17, 58, 643
TspRI CASTG 6 cut(s) 265, 325, 866, 1086, 1230, 1612
Vha464I CTTAAG 1 cut(s) 491
VpaK11BI GGWCC 1 cut(s) 785
VspI ATTAAT 1 cut(s) 1587
XagI CCTNNNNNAGG 1 cut(s) 1489
XapI RAATTY 4 cut(s) 731, 740, 854, 1255
XceI RCATGY 1 cut(s) 1244
XcmI CCANNNNNNNNNTGG 2 cut(s) 564, 1051
XhoI CTCGAG 1 cut(s) 124
XmiI GTMKAC 1 cut(s) 1211
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 1 cut(s) 1380
Zsp2I ATGCAT 1 cut(s) 1560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.