MD09G1275700.v1.1

Glycosyl hydrolase family 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006678
Physical Location & Seq
Forward (+)
35322018 .. 35326518
4501 bp
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UTR
Exon/CDS
Intron
MD09G1275700.v1.1.491

Sequence Viewer

Length: 249 bp
ATGTTGCAGCGTATTATGGGAATAATATCAGAAATTGAAGTTGGATTAGGTCCGTGTGGAGAGCTACGGTATCCTTATCCTGAACGGCATGGTTGGAAATATCCTAGTATTGGTGAATTCCAGTGTTATGACCGGTACTTGATGAAGAATCTGACTCAGGCTGCAAAAGCAAGGGCCACTCCTTCTAGGCCTGTAGCTCATTTGATTTTTACCATTACGCTTGGTAGATATATGATCCTTATTGAATGA

Protein Analysis

83

Amino Acids

9.47

Weight (kDa)

9.06

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 6 - 55 5.4e-16 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 229
AcsI RAATTY 1 cut(s) 116
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 110
AgeI ACCGGT 1 cut(s) 132
AgsI TTSAA 2 cut(s) 38, 245
AjuI GAANNNNNNNTTGG 2 cut(s) 24, 56
AluBI AGCT 2 cut(s) 64, 197
AluI AGCT 2 cut(s) 64, 197
AlwI GGATC 1 cut(s) 229
AoxI GGCC 2 cut(s) 174, 188
ApeKI GCWGC 2 cut(s) 7, 161
ApoI RAATTY 1 cut(s) 116
AsiGI ACCGGT 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 50, 174
AsuHPI GGTGA 1 cut(s) 125
AvaII GGWCC 1 cut(s) 50
BbvI GCAGC 2 cut(s) 19, 148
BceAI ACGGC 1 cut(s) 101
BciVI GTATCC 1 cut(s) 81
BfaI CTAG 2 cut(s) 105, 186
BfmI CTRYAG 1 cut(s) 192
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 2 cut(s) 8, 162
BlsI GCNGC 2 cut(s) 9, 163
Bme18I GGWCC 1 cut(s) 50
BmgT120I GGNCC 2 cut(s) 50, 174
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 132
Bse1I ACTGG 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 110
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 1 cut(s) 121
BseXI GCAGC 2 cut(s) 19, 148
BshFI GGCC 2 cut(s) 176, 190
BshTI ACCGGT 1 cut(s) 132
BsiSI CCGG 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 110
BsnI GGCC 2 cut(s) 176, 190
Bsp143I GATC 1 cut(s) 234
BspANI GGCC 2 cut(s) 176, 190
BspCNI CTCAG 1 cut(s) 169
BspPI GGATC 1 cut(s) 229
BsrFI RCCGGY 1 cut(s) 132
BsrI ACTGG 1 cut(s) 121
BssAI RCCGGY 1 cut(s) 132
BssMI GATC 1 cut(s) 234
Bst4CI ACNGT 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 156
BstKTI GATC 1 cut(s) 237
BstMBI GATC 1 cut(s) 234
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 19, 148
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 2 cut(s) 176, 190
BtsIMutI CAGTG 1 cut(s) 128
Cfr10I RCCGGY 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 50, 174
Csp6I GTAC 1 cut(s) 136
CspAI ACCGGT 1 cut(s) 132
CviAII CATG 1 cut(s) 89
CviJI RGCY 5 cut(s) 64, 161, 176, 190, 197
CviKI_1 RGCY 5 cut(s) 64, 161, 176, 190, 197
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 1 cut(s) 156
DpnI GATC 1 cut(s) 236
DpnII GATC 1 cut(s) 234
Eco147I AGGCCT 1 cut(s) 190
Eco47I GGWCC 1 cut(s) 50
EcoRI GAATTC 1 cut(s) 116
FaeI CATG 1 cut(s) 92
FaiI YATR 5 cut(s) 17, 90, 129, 231, 233
FatI CATG 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 8, 162
Fsp4HI GCNGC 2 cut(s) 8, 162
FspBI CTAG 2 cut(s) 105, 186
GluI GCNGC 2 cut(s) 8, 162
HaeIII GGCC 2 cut(s) 176, 190
HapII CCGG 1 cut(s) 133
Hin1II CATG 1 cut(s) 92
HinfI GANTC 2 cut(s) 148, 154
HpaII CCGG 1 cut(s) 133
HphI GGTGA 1 cut(s) 125
Hpy188I TCNGA 2 cut(s) 31, 153
Hpy188III TCNNGA 1 cut(s) 80
HpyAV CCTTC 1 cut(s) 192
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 7, 164
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 1 cut(s) 156
Hsp92II CATG 1 cut(s) 92
Kzo9I GATC 1 cut(s) 234
LpnPI CCDG 5 cut(s) 93, 134, 143, 146, 204
Lsp1109I GCAGC 2 cut(s) 19, 148
MaeI CTAG 2 cut(s) 105, 186
MalI GATC 1 cut(s) 236
MboI GATC 1 cut(s) 234
MboII GAAGA 1 cut(s) 157
MluCI AATT 2 cut(s) 33, 116
MlyI GAGTC 1 cut(s) 148
MmeI TCCRAC 2 cut(s) 22, 74
MspI CCGG 1 cut(s) 133
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 1 cut(s) 234
NlaIII CATG 1 cut(s) 92
PceI AGGCCT 1 cut(s) 190
PfeI GAWTC 1 cut(s) 148
PinAI ACCGGT 1 cut(s) 132
PkrI GCNGC 2 cut(s) 9, 163
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PspPI GGNCC 2 cut(s) 50, 174
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
SatI GCNGC 2 cut(s) 8, 162
Sau3AI GATC 1 cut(s) 234
Sau96I GGNCC 2 cut(s) 50, 174
SchI GAGTC 1 cut(s) 148
SetI ASST 3 cut(s) 52, 66, 199
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 50
Sse9I AATT 2 cut(s) 33, 116
SseBI AGGCCT 1 cut(s) 190
SspMI CTAG 2 cut(s) 105, 186
StuI AGGCCT 1 cut(s) 190
TaaI ACNGT 1 cut(s) 69
TasI AATT 2 cut(s) 33, 116
TfiI GAWTC 1 cut(s) 148
TscAI CASTG 1 cut(s) 128
TseI GCWGC 2 cut(s) 7, 161
TspDTI ATGAA 1 cut(s) 158
TspGWI ACGGA 1 cut(s) 42
TspRI CASTG 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 50
XapI RAATTY 1 cut(s) 116
XspI CTAG 2 cut(s) 105, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.