MD07G1137800.v1.1

Glycosyl hydrolase family 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006678
Physical Location & Seq
Forward (+)
20080253 .. 20092797
12545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1137800.v1.1.491

Sequence Viewer

Length: 1647 bp
ATGGCACAATTCTCGGCTCCTCAAGTATCCAGTACGCTGAGGGCGCCACGCCGGCCAATTGCTTGTTGCAAGGCGACGACAATGGCTCGGTTGTCGTCCTGCACACAGACCGCGGTAGCATCCCTTAAGCTCACTCCGGGGTTTCGGGGCTTGCCGGAGCTCAATTCGTGTCATTTGAGAGTATTGGGGAGTGATCGGTCTTGGAGTTTCGCCGTGGCAAGAGACAGTGCTGAGGGGACTGCGGTGGGTGATCCTGTGGAAAATGCCGGTCACAATCAGGCTGAAGATTCTTCAACAGAAAAGTTTGAGGAGCGTGATTTTACCGGCACGCCGTATGTTCCTGTGTATGTGATGTTACCGTTGGGTGTGATCAATATGAACTTTGAGCTAGTTGAGCCAGAAGCTCTGCGAAATCAACTACAAGTGTTGAAGTCGGTTGGAGTTGATGGTGTTATGGTTGATTGCTGGTGGGGGATAGTAGAGGCGCACAATCCACAGCAATATAATTGGAATGGCTACAAGAGACTTTTTCAGATTGTGCGTGATCTTAACCTTAAGCTACAGGTCGTGATGTCATTCCATGAATGTGGAGGCAATGTTGGGGATGATGTACATATCCCACTTCCTCACTGGGTGACAGAAATTGGCCAAAAGAATCCTGATATATATTTTACAAATAAAGAAGGGAAACGCAATGCTGAATGTCTCACATGGGGAATTGATAAGGAGCGAGTTTTAAGAGGCCGTACTGCTGTTGAGGTTTACTTCGACTACATGAGGAGCTTCAGAGTTGAATTTAATGAATTTTTTGAGGAAGGAATAATATCAGAAATTGAAGTTGGATTAGGTCCATGTGGAGAGCTACGTTATCCTTCTTATCCTGAACAGCATGGTTGGAAATATCCTGGTATTGGTGAATTCCAGTGTTATGACCGATACTTGATGAAGTATCTGACGCAGGCTGCAGAAGCAAGGGGCCACTCCTTTTGGGCCAGAGCACCAGATAATGCAGGTTCTTATAGTTCCCAACCACATGAGACAGGGTTTTTCCGTGACGGAGGTGATTATGATAGCTACTATGGCAGATTCTTCCTCAATTGGTACTCCCGTGTTTTGATTGATCATGGTGATCGTGTACTTGCTCTGGCCAATTTAGCTTTCGAAGGCACACACATTGCTACAAAGGTATCAGGTATCCACTGGTGGTACAAGACTGCCAGCCATCCTGCTGAATTAACTGCTGGATTTTACAACTCCTGCAATCGTGATGGGTATGTGCCAATATCAGAAATGTTAAAAAAGCATGAGGCTGCCCTGAATTTCACATGTGTTGAAATGCGCACGTCAGACCAGCATCAGGGCTTTCCGGAAGCACTGGCAGACCCTGAGGGATTGGTTTGGCAGGTGCTGAATGCTGCATGGGATGCTGACATTCCAGTCGCTAGTGAGAATGCTCTTGCATGCTTTGATAGAGAAGGCTACAACAAGATATTAGAAAATGCTAAGCCCAGGAATGATCCAGATGGCAGACATTTATCCGCATTTACCTACCTCAGATTAAGCCCAGTTCTCATTGAGAGGCATAACTTAATGGAGTTCGAACGGTTTGTCAAGAGAATGCATGGAGAAGCCGCTCATGATGTTTAG

Protein Analysis

549

Amino Acids

62.09

Weight (kDa)

5.6

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 116 - 528 3.5e-133 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1393
AasI GACNNNNNNGTC 1 cut(s) 1436
Acc16I TGCGCA 1 cut(s) 1340
Acc36I ACCTGC 2 cut(s) 1001, 1393
AccB1I GGYRCC 1 cut(s) 43
AccBSI CCGCTC 1 cut(s) 1634
AccII CGCG 1 cut(s) 113
AccIII TCCGGA 1 cut(s) 1366
AciI CCGC 5 cut(s) 111, 113, 242, 1539, 1632
AclWI GGATC 2 cut(s) 245, 1511
AcoI YGGCCR 3 cut(s) 53, 646, 1146
AcsI RAATTY 4 cut(s) 794, 803, 917, 1318
AcuI CTGAAG 2 cut(s) 303, 769
AcyI GRCGYC 1 cut(s) 44
AdeI CACNNNGTG 1 cut(s) 634
AfaI GTAC 6 cut(s) 34, 612, 748, 1103, 1137, 1208
AfiI CCNNNNNNNGG 2 cut(s) 911, 1357
AflII CTTAAG 2 cut(s) 125, 554
AflIII ACRYGT 1 cut(s) 1325
AgsI TTSAA 5 cut(s) 294, 430, 794, 836, 1334
AjiI CACGTC 1 cut(s) 1344
AjnI CCWGG 2 cut(s) 904, 1508
AjuI GAANNNNNNNTTGG 4 cut(s) 822, 854, 1142, 1174
AluBI AGCT 9 cut(s) 130, 160, 388, 404, 559, 783, 862, 1074, 1157
AluI AGCT 9 cut(s) 130, 160, 388, 404, 559, 783, 862, 1074, 1157
Alw21I GWGCWC 2 cut(s) 162, 1000
Alw26I GTCTC 4 cut(s) 216, 517, 710, 1031
AlwI GGATC 2 cut(s) 245, 1511
AlwNI CAGNNNCTG 2 cut(s) 1385, 1408
Aor13HI TCCGGA 1 cut(s) 1366
AoxI GGCC 6 cut(s) 53, 646, 742, 976, 990, 1146
ApeKI GCWGC 3 cut(s) 962, 1310, 1415
ApoI RAATTY 4 cut(s) 794, 803, 917, 1318
ArsI GACNNNNNNTTYG 2 cut(s) 1593, 1625
AspLEI GCGC 3 cut(s) 46, 487, 1341
AspS9I GGNCC 3 cut(s) 848, 976, 990
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 5 cut(s) 260, 646, 926, 1073, 1139
AsuII TTCGAA 2 cut(s) 1161, 1599
AvaII GGWCC 1 cut(s) 848
AxyI CCTNAGG 1 cut(s) 1386
BaeI ACNNNNGTAYC 4 cut(s) 1093, 1126, 1170, 1203
BalI TGGCCA 2 cut(s) 648, 1148
BanI GGYRCC 1 cut(s) 43
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 2 cut(s) 162, 1000
BbvCI CCTCAGC 2 cut(s) 38, 231
BbvI GCAGC 3 cut(s) 949, 1297, 1402
BccI CCATC 4 cut(s) 440, 1230, 1262, 1517
BceAI ACGGC 3 cut(s) 197, 316, 729
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 2 cut(s) 906, 1510
BciVI GTATCC 2 cut(s) 37, 1205
BclI TGATCA 2 cut(s) 369, 1120
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 4 cut(s) 216, 517, 710, 1031
BfaI CTAG 2 cut(s) 389, 1443
BfmI CTRYAG 2 cut(s) 560, 963
BfoI RGCGCY 1 cut(s) 47
BfrI CTTAAG 2 cut(s) 125, 554
BfuAI ACCTGC 2 cut(s) 1001, 1393
BfuI GTATCC 2 cut(s) 37, 1205
BglI GCCNNNNNGGC 1 cut(s) 52
BisI GCNGC 4 cut(s) 963, 1311, 1416, 1632
BlpI GCTNAGC 1 cut(s) 1503
BlsI GCNGC 4 cut(s) 964, 1312, 1417, 1633
Bme1390I CCNGG 3 cut(s) 138, 906, 1510
Bme18I GGWCC 1 cut(s) 848
BmgBI CACGTC 1 cut(s) 1344
BmgT120I GGNCC 3 cut(s) 848, 976, 990
BmiI GGNNCC 3 cut(s) 18, 45, 977
BmrFI CCNGG 3 cut(s) 138, 906, 1510
BmrI ACTGGG 2 cut(s) 640, 1559
BmsI GCATC 3 cut(s) 128, 1363, 1414
BmuI ACTGGG 2 cut(s) 640, 1559
Bpu10I CCTNAGC 2 cut(s) 38, 231
Bpu1102I GCTNAGC 1 cut(s) 1503
Bpu14I TTCGAA 2 cut(s) 1161, 1599
BpuEI CTTGAG 1 cut(s) 6
BpuMI CCSGG 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 44
BsaJI CCNNGG 4 cut(s) 111, 137, 213, 1508
BsaWI WCCGGW 1 cut(s) 1366
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 2 cut(s) 911, 1357
Bse118I RCCGGY 3 cut(s) 51, 266, 323
Bse1I ACTGG 7 cut(s) 30, 635, 922, 1205, 1380, 1436, 1565
Bse21I CCTNAGG 1 cut(s) 1386
Bse3DI GCAATG 3 cut(s) 601, 700, 1173
BseAI TCCGGA 1 cut(s) 1366
BseBI CCWGG 2 cut(s) 906, 1510
BseDI CCNNGG 4 cut(s) 111, 137, 213, 1508
BseGI GGATG 4 cut(s) 119, 610, 1222, 1429
BseLI CCNNNNNNNGG 2 cut(s) 911, 1357
BseMI GCAATG 3 cut(s) 601, 700, 1173
BseMII CTCAG 4 cut(s) 29, 222, 1377, 1567
BseNI ACTGG 7 cut(s) 30, 635, 922, 1205, 1380, 1436, 1565
BseRI GAGGAG 3 cut(s) 9, 323, 793
BseXI GCAGC 3 cut(s) 949, 1297, 1402
BsgI GTGCAG 1 cut(s) 85
Bsh1236I CGCG 1 cut(s) 113
BshFI GGCC 6 cut(s) 55, 648, 744, 978, 992, 1148
BshNI GGYRCC 1 cut(s) 43
BsiHKAI GWGCWC 2 cut(s) 162, 1000
BsiSI CCGG 6 cut(s) 52, 137, 155, 267, 324, 1367
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 911, 1357
BsmAI GTCTC 4 cut(s) 216, 517, 710, 1031
BsmFI GGGAC 1 cut(s) 250
BsmI GAATGC 3 cut(s) 1417, 1456, 1623
BsnI GGCC 6 cut(s) 55, 648, 744, 978, 992, 1148
Bsp119I TTCGAA 2 cut(s) 1161, 1599
Bsp1286I GDGCHC 2 cut(s) 162, 1000
Bsp13I TCCGGA 1 cut(s) 1366
Bsp1407I TGTACA 1 cut(s) 610
Bsp143I GATC 7 cut(s) 193, 250, 369, 544, 1120, 1129, 1516
Bsp1720I GCTNAGC 1 cut(s) 1503
BspACI CCGC 5 cut(s) 111, 113, 242, 1539, 1632
BspANI GGCC 6 cut(s) 55, 648, 744, 978, 992, 1148
BspCNI CTCAG 4 cut(s) 30, 223, 1378, 1566
BspEI TCCGGA 1 cut(s) 1366
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 1 cut(s) 1636
BspLI GGNNCC 3 cut(s) 18, 45, 977
BspMAI CTGCAG 1 cut(s) 967
BspMI ACCTGC 2 cut(s) 1001, 1393
BspPI GGATC 2 cut(s) 245, 1511
BspT104I TTCGAA 2 cut(s) 1161, 1599
BspT107I GGYRCC 1 cut(s) 43
BspTI CTTAAG 2 cut(s) 125, 554
BsrBI CCGCTC 1 cut(s) 1634
BsrDI GCAATG 3 cut(s) 601, 700, 1173
BsrFI RCCGGY 3 cut(s) 51, 266, 323
BsrGI TGTACA 1 cut(s) 610
BsrI ACTGG 7 cut(s) 30, 635, 922, 1205, 1380, 1436, 1565
BssAI RCCGGY 3 cut(s) 51, 266, 323
BssECI CCNNGG 4 cut(s) 111, 137, 213, 1508
BssMI GATC 7 cut(s) 193, 250, 369, 544, 1120, 1129, 1516
BssNI GRCGYC 1 cut(s) 44
Bst2UI CCWGG 2 cut(s) 906, 1510
Bst4CI ACNGT 3 cut(s) 227, 360, 1605
BstACI GRCGYC 1 cut(s) 44
BstAFI CTTAAG 2 cut(s) 125, 554
BstAPI GCANNNNNTGC 1 cut(s) 1424
BstAUI TGTACA 1 cut(s) 610
BstBI TTCGAA 2 cut(s) 1161, 1599
BstC8I GCNNGC 6 cut(s) 53, 152, 329, 960, 1219, 1462
BstDEI CTNAG 5 cut(s) 38, 231, 1386, 1503, 1553
BstDSI CCRYGG 2 cut(s) 111, 213
BstF5I GGATG 4 cut(s) 119, 610, 1222, 1429
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 47
BstHHI GCGC 3 cut(s) 46, 487, 1341
BstKTI GATC 7 cut(s) 196, 253, 372, 547, 1123, 1132, 1519
BstMAI GTCTC 4 cut(s) 216, 517, 710, 1031
BstMBI GATC 7 cut(s) 193, 250, 369, 544, 1120, 1129, 1516
BstMWI GCNNNNNNNGC 7 cut(s) 43, 52, 394, 968, 1080, 1154, 1424
BstNI CCWGG 2 cut(s) 906, 1510
BstNSI RCATGY 2 cut(s) 1329, 1464
BstSCI CCNGG 3 cut(s) 136, 904, 1508
BstSFI CTRYAG 2 cut(s) 560, 963
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 949, 1297, 1402
BstXI CCANNNNNNTGG 1 cut(s) 587
Bsu36I CCTNAGG 1 cut(s) 1386
BsuI GTATCC 2 cut(s) 37, 1205
BsuRI GGCC 6 cut(s) 55, 648, 744, 978, 992, 1148
BtgI CCRYGG 2 cut(s) 111, 213
BtrI CACGTC 1 cut(s) 1344
BtsCI GGATG 4 cut(s) 119, 610, 1222, 1429
BtsIMutI CAGTG 5 cut(s) 232, 628, 929, 1198, 1373
BveI ACCTGC 2 cut(s) 1001, 1393
Cac8I GCNNGC 6 cut(s) 53, 152, 329, 960, 1219, 1462
CaiI CAGNNNCTG 2 cut(s) 1385, 1408
CciI TCATGA 1 cut(s) 1636
CfoI GCGC 3 cut(s) 46, 487, 1341
Cfr10I RCCGGY 3 cut(s) 51, 266, 323
Cfr13I GGNCC 3 cut(s) 848, 976, 990
Cfr42I CCGCGG 1 cut(s) 114
CseI GACGC 1 cut(s) 964
Csp6I GTAC 6 cut(s) 33, 611, 747, 1102, 1136, 1207
CspCI CAANNNNNGTGG 2 cut(s) 968, 1003
CviQI GTAC 6 cut(s) 33, 611, 747, 1102, 1136, 1207
DdeI CTNAG 5 cut(s) 38, 231, 1386, 1503, 1553
DinI GGCGCC 1 cut(s) 45
DpnI GATC 7 cut(s) 195, 252, 371, 546, 1122, 1131, 1518
DpnII GATC 7 cut(s) 193, 250, 369, 544, 1120, 1129, 1516
DraIII CACNNNGTG 1 cut(s) 634
DrdI GACNNNNNNGTC 1 cut(s) 1436
DseDI GACNNNNNNGTC 1 cut(s) 1436
EaeI YGGCCR 3 cut(s) 53, 646, 1146
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 848
Eco53kI GAGCTC 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 303, 769
Eco81I CCTNAGG 1 cut(s) 1386
EcoICRI GAGCTC 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 917
EcoRII CCWGG 2 cut(s) 904, 1508
EcoT22I ATGCAT 1 cut(s) 1623
EcoT38I GRGCYC 1 cut(s) 162
EgeI GGCGCC 1 cut(s) 45
EheI GGCGCC 1 cut(s) 45
FaqI GGGAC 1 cut(s) 250
FbaI TGATCA 2 cut(s) 369, 1120
Fnu4HI GCNGC 4 cut(s) 963, 1311, 1416, 1632
FokI GGATG 4 cut(s) 106, 617, 1209, 1436
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 4 cut(s) 963, 1311, 1416, 1632
FspAI RTGCGCAY 1 cut(s) 1340
FspBI CTAG 2 cut(s) 389, 1443
FspI TGCGCA 1 cut(s) 1340
GlaI GCGC 3 cut(s) 45, 486, 1340
GluI GCNGC 4 cut(s) 963, 1311, 1416, 1632
HaeII RGCGCY 1 cut(s) 47
HaeIII GGCC 6 cut(s) 55, 648, 744, 978, 992, 1148
HapII CCGG 6 cut(s) 52, 137, 155, 267, 324, 1367
HgaI GACGC 1 cut(s) 964
HhaI GCGC 3 cut(s) 46, 487, 1341
Hin1I GRCGYC 1 cut(s) 44
Hin6I GCGC 3 cut(s) 44, 485, 1339
HinP1I GCGC 3 cut(s) 44, 485, 1339
HinfI GANTC 3 cut(s) 287, 655, 1086
HpaII CCGG 6 cut(s) 52, 137, 155, 267, 324, 1367
HphI GGTGA 5 cut(s) 260, 646, 926, 1073, 1139
Hpy166II GTNNAC 2 cut(s) 763, 1136
Hpy188I TCNGA 7 cut(s) 534, 788, 829, 954, 1288, 1348, 1556
Hpy188III TCNNGA 8 cut(s) 568, 659, 881, 1265, 1367, 1520, 1612, 1637
Hpy8I GTNNAC 2 cut(s) 763, 1136
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 5 cut(s) 677, 809, 882, 1157, 1469
HpyCH4III ACNGT 3 cut(s) 227, 360, 1605
HpyCH4IV ACGT 2 cut(s) 865, 1343
HpyCH4V TGCA 8 cut(s) 69, 102, 965, 1010, 1260, 1418, 1460, 1621
HpyF10VI GCNNNNNNNGC 7 cut(s) 43, 52, 394, 968, 1080, 1154, 1424
HpyF3I CTNAG 5 cut(s) 38, 231, 1386, 1503, 1553
HpySE526I ACGT 2 cut(s) 865, 1343
Hsp92I GRCGYC 1 cut(s) 44
HspAI GCGC 3 cut(s) 44, 485, 1339
KasI GGCGCC 1 cut(s) 43
Kpn2I TCCGGA 1 cut(s) 1366
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
Ksp22I TGATCA 2 cut(s) 369, 1120
KspI CCGCGG 1 cut(s) 114
Kzo9I GATC 7 cut(s) 193, 250, 369, 544, 1120, 1129, 1516
LmnI GCTCC 5 cut(s) 22, 157, 310, 727, 780
Lsp1109I GCAGC 3 cut(s) 949, 1297, 1402
LweI GCATC 3 cut(s) 128, 1363, 1414
MaeI CTAG 2 cut(s) 389, 1443
MaeII ACGT 2 cut(s) 865, 1343
MaeIII GTNAC 4 cut(s) 269, 354, 634, 1052
MalI GATC 7 cut(s) 195, 252, 371, 546, 1122, 1131, 1518
MbiI CCGCTC 1 cut(s) 1634
MboI GATC 7 cut(s) 193, 250, 369, 544, 1120, 1129, 1516
MboII GAAGA 3 cut(s) 282, 296, 1081
MfeI CAATTG 2 cut(s) 57, 1096
MhlI GDGCHC 2 cut(s) 162, 1000
MlsI TGGCCA 2 cut(s) 648, 1148
MluNI TGGCCA 2 cut(s) 648, 1148
Mly113I GGCGCC 1 cut(s) 44
MmeI TCCRAC 3 cut(s) 418, 820, 875
Mox20I TGGCCA 2 cut(s) 648, 1148
Mph1103I ATGCAT 1 cut(s) 1623
MroI TCCGGA 1 cut(s) 1366
MroNI GCCGGC 1 cut(s) 51
MscI TGGCCA 2 cut(s) 648, 1148
MseI TTAA 9 cut(s) 126, 549, 555, 737, 798, 1235, 1295, 1559, 1589
MslI CAYNNNNRTG 1 cut(s) 585
Msp20I TGGCCA 2 cut(s) 648, 1148
MspA1I CMGCKG 1 cut(s) 113
MspCI CTTAAG 2 cut(s) 125, 554
MspI CCGG 6 cut(s) 52, 137, 155, 267, 324, 1367
MspR9I CCNGG 3 cut(s) 138, 906, 1510
MunI CAATTG 2 cut(s) 57, 1096
Mva1269I GAATGC 3 cut(s) 1417, 1456, 1623
MvaI CCWGG 2 cut(s) 906, 1510
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 7 cut(s) 43, 52, 394, 968, 1080, 1154, 1424
NaeI GCCGGC 1 cut(s) 53
NarI GGCGCC 1 cut(s) 44
NciI CCSGG 1 cut(s) 138
NdeII GATC 7 cut(s) 193, 250, 369, 544, 1120, 1129, 1516
NgoMIV GCCGGC 1 cut(s) 51
NlaIV GGNNCC 3 cut(s) 18, 45, 977
NmuCI GTSAC 3 cut(s) 269, 634, 1052
NsbI TGCGCA 1 cut(s) 1340
NsiI ATGCAT 1 cut(s) 1623
NspI RCATGY 2 cut(s) 1329, 1464
NspV TTCGAA 2 cut(s) 1161, 1599
PaeI GCATGC 1 cut(s) 1464
PagI TCATGA 1 cut(s) 1636
PaqCI CACCTGC 1 cut(s) 1393
PciI ACATGT 1 cut(s) 1325
PctI GAATGC 3 cut(s) 1417, 1456, 1623
PdiI GCCGGC 1 cut(s) 53
PfeI GAWTC 3 cut(s) 287, 655, 1086
PkrI GCNGC 4 cut(s) 964, 1312, 1417, 1633
PluTI GGCGCC 1 cut(s) 47
PscI ACATGT 1 cut(s) 1325
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 904, 1508
PspGI CCWGG 2 cut(s) 904, 1508
PspN4I GGNNCC 3 cut(s) 18, 45, 977
PspPI GGNCC 3 cut(s) 848, 976, 990
PstI CTGCAG 1 cut(s) 967
PstNI CAGNNNCTG 2 cut(s) 1385, 1408
RsaI GTAC 6 cut(s) 34, 612, 748, 1103, 1137, 1208
RsaNI GTAC 6 cut(s) 33, 611, 747, 1102, 1136, 1207
RseI CAYNNNNRTG 1 cut(s) 585
SacI GAGCTC 1 cut(s) 162
SacII CCGCGG 1 cut(s) 114
SaqAI TTAA 9 cut(s) 126, 549, 555, 737, 798, 1235, 1295, 1559, 1589
SatI GCNGC 4 cut(s) 963, 1311, 1416, 1632
Sau3AI GATC 7 cut(s) 193, 250, 369, 544, 1120, 1129, 1516
Sau96I GGNCC 3 cut(s) 848, 976, 990
ScrFI CCNGG 3 cut(s) 138, 906, 1510
SduI GDGCHC 2 cut(s) 162, 1000
SfaNI GCATC 3 cut(s) 128, 1363, 1414
SfcI CTRYAG 2 cut(s) 560, 963
SfoI GGCGCC 1 cut(s) 45
Sfr303I CCGCGG 1 cut(s) 114
SfuI TTCGAA 2 cut(s) 1161, 1599
SgrBI CCGCGG 1 cut(s) 114
SinI GGWCC 1 cut(s) 848
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 3 cut(s) 21, 125, 554
SmoI CTYRAG 3 cut(s) 21, 125, 554
SphI GCATGC 1 cut(s) 1464
SsiI CCGC 5 cut(s) 111, 113, 242, 1539, 1632
SspDI GGCGCC 1 cut(s) 43
SspMI CTAG 2 cut(s) 389, 1443
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 136, 904, 1508
TaaI ACNGT 3 cut(s) 227, 360, 1605
TaiI ACGT 2 cut(s) 868, 1346
TaqI TCGA 3 cut(s) 768, 1161, 1599
TaqII GACCGA 2 cut(s) 186, 948
TatI WGTACW 2 cut(s) 610, 1135
TauI GCSGC 1 cut(s) 1634
TfiI GAWTC 3 cut(s) 287, 655, 1086
Tru1I TTAA 9 cut(s) 126, 549, 555, 737, 798, 1235, 1295, 1559, 1589
Tru9I TTAA 9 cut(s) 126, 549, 555, 737, 798, 1235, 1295, 1559, 1589
TscAI CASTG 5 cut(s) 232, 635, 929, 1205, 1380
TseFI GTSAC 3 cut(s) 269, 634, 1052
TseI GCWGC 3 cut(s) 962, 1310, 1415
Tsp45I GTSAC 3 cut(s) 269, 634, 1052
TspDTI ATGAA 4 cut(s) 392, 597, 816, 959
TspGWI ACGGA 2 cut(s) 1040, 1071
TspRI CASTG 5 cut(s) 232, 635, 929, 1205, 1380
Vha464I CTTAAG 2 cut(s) 125, 554
VpaK11BI GGWCC 1 cut(s) 848
XapI RAATTY 4 cut(s) 794, 803, 917, 1318
XceI RCATGY 2 cut(s) 1329, 1464
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XspI CTAG 2 cut(s) 389, 1443
Zsp2I ATGCAT 1 cut(s) 1623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.