Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0013949

Physical Location & Seq

Genomic Coordinates Reverse (-)

522530 .. 524249

Gene Structure 1720 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G01250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGGCCGACGATTGGGATCTCCACGCCGTAGTCAGAGGCTGCTCAGCCGTAAGCTCATCAGCTACTACCACCGTATATTCCCCCGGCGTTTCATCTCACACAAACCCTATATTCACCGTCGGACGACAAAGTAATGCCGTCTCCTTCGGAGAGATTCGAGATCTCTACACACCGTTCACACAAGAATCTGTCGTCTCTTCGTTTTCTTGTATAAACTACCCAGAAGAACCTAGAAAGCCACAGAACCAGAAACGTCCTCTTTCTCTCTCTGCTTCTTCCGGTAGCGTCACTAGCAAACCCAGTGGCTCCAATACCTCTAGATCTAAAAGAAGAAAGATACAGCATAAGAAAGTGTGCCATGTAGCAGCAGAAGCTTTAAACTCCGATGTCTGGGCATGGCGAAAGTACGGACAGAAACCCATCAAAGGTTCACCATATCCAAGAGGATACTACAGATGTAGTACATCAAAAGGTTGTTTAGCCCGTAAACAAGTGGAGCGAAATAGATCCGACCCGAAGATGTTTATCGTCACTTACACGGCGGAGCATAATCATCCAGCTCCGACACACCGTAATTCTCTCGCCGGAAGCACACGTCAGAAACCATCCGATCAACAGACGAGTAAATCTCCGACGACCACTATTGCTACTTATTCATCGTCTCCGGTGACTTCAGCCGACGAATTTGTTTTGCCTGTTGAGGATCATCTAGCGGTGGGAGATCTTGACGGAGAAGAAGATCTGTTATCTTTGTCGGATACGGTGGTTAGCGATGATTTCTTCGATGGGTTAGAGGAATTCGCAGCCGGAGATAGCTTTTCCGGGAACTCGGCTCCGGCGAGTTTTGATCTCTCTTGGGTTGTGAACAGTGCCGCCACTACCACCGGAGGAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0009889 GO:0010033 GO:0010150 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090693 GO:0099402 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

298

Amino Acids

32.26

Weight (kDa)

7.08

Isoelectric Point (pI)

44.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	129 - 186	4.9e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01250
fragaria_vesca	FvH4_7g15350
malus_domestica	MD01G1071300.v1.1 MD07G1131400.v1.1
prunus_persica	Prupe.2G177400_v2.0.a1
pyrus_communis	pycom01g10030
rosa_chinensis	RchiOBHm_Chr1g0357671
rosa_laevigata	RLG00000028008
rosa_multiflora	Rmu_co8407323.1_g000001 Rmu_ssc0000425.1_g000001
rosa_roxburghii	Rroxscaffold_4G00298610
rosa_rugosa	Rorug01G0257600
rosa_samantha	Rh1AG271600 Rh1BG238700 Rh1CG255500 Rh1DG267100
rosa_wichuraiana	Rw1G024220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	542, 713, 873
AclWI	GGATC	3 cut(s)	24, 501, 711
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	683, 797
AcuI	CTGAAG	1 cut(s)	657
AfaI	GTAC	2 cut(s)	407, 463
AfiI	CCNNNNNNNGG	3 cut(s)	12, 390, 425
AflIII	ACRYGT	1 cut(s)	593
AjiI	CACGTC	1 cut(s)	596
AluBI	AGCT	5 cut(s)	54, 62, 374, 560, 816
AluI	AGCT	5 cut(s)	54, 62, 374, 560, 816
Alw26I	GTCTC	3 cut(s)	145, 199, 666
AlwI	GGATC	3 cut(s)	24, 501, 711
AlwNI	CAGNNNCTG	1 cut(s)	39
AoxI	GGCC	1 cut(s)	3
ApeKI	GCWGC	3 cut(s)	39, 365, 803
ApoI	RAATTY	2 cut(s)	683, 797
AsuC2I	CCSGG	2 cut(s)	84, 823
AsuHPI	GGTGA	3 cut(s)	106, 423, 679
BaeI	ACNNNNGTAYC	2 cut(s)	750, 783
BbvI	GCAGC	3 cut(s)	26, 377, 815
BccI	CCATC	3 cut(s)	428, 613, 779
BceAI	ACGGC	4 cut(s)	11, 32, 122, 555
BciVI	GTATCC	2 cut(s)	440, 751
BcnI	CCSGG	2 cut(s)	84, 823
BcoDI	GTCTC	3 cut(s)	145, 199, 666
BfaI	CTAG	4 cut(s)	231, 291, 318, 710
BfmI	CTRYAG	1 cut(s)	451
BfuI	GTATCC	2 cut(s)	440, 751
BglII	AGATCT	4 cut(s)	160, 320, 721, 739
BisI	GCNGC	4 cut(s)	40, 366, 804, 873
BlpI	GCTNAGC	1 cut(s)	43
BlsI	GCNGC	4 cut(s)	41, 367, 805, 874
Bme1390I	CCNGG	2 cut(s)	84, 823
BmgBI	CACGTC	1 cut(s)	596
BmiI	GGNNCC	2 cut(s)	307, 834
BmrFI	CCNGG	2 cut(s)	84, 823
BmrI	ACTGGG	1 cut(s)	294
BmuI	ACTGGG	1 cut(s)	294
BplI	GAGNNNNNCTC	2 cut(s)	613, 645
Bpu1102I	GCTNAGC	1 cut(s)	43
BpuMI	CCSGG	2 cut(s)	84, 823
BsaBI	GATNNNNATC	2 cut(s)	15, 524
BsaJI	CCNNGG	1 cut(s)	82
BsaWI	WCCGGW	3 cut(s)	278, 664, 884
Bsc4I	CCNNNNNNNGG	3 cut(s)	12, 390, 425
Bse1I	ACTGG	1 cut(s)	300
Bse8I	GATNNNNATC	2 cut(s)	15, 524
BseDI	CCNNGG	1 cut(s)	82
BseGI	GGATG	2 cut(s)	553, 605
BseJI	GATNNNNATC	2 cut(s)	15, 524
BseLI	CCNNNNNNNGG	3 cut(s)	12, 390, 425
BseMII	CTCAG	1 cut(s)	57
BseNI	ACTGG	1 cut(s)	300
BseXI	GCAGC	3 cut(s)	26, 377, 815
BshFI	GGCC	1 cut(s)	5
BsiSI	CCGG	8 cut(s)	84, 279, 585, 665, 807, 822, 836, 885
BslI	CCNNNNNNNGG	3 cut(s)	12, 390, 425
BsmAI	GTCTC	3 cut(s)	145, 199, 666
BsmBI	CGTCTC	3 cut(s)	145, 199, 666
BsnI	GGCC	1 cut(s)	5
Bsp143I	GATC	9 cut(s)	16, 160, 320, 506, 610, 703, 721, 739, 847
Bsp1720I	GCTNAGC	1 cut(s)	43
BspACI	CCGC	3 cut(s)	542, 713, 873
BspANI	GGCC	1 cut(s)	5
BspCNI	CTCAG	1 cut(s)	56
BspLI	GGNNCC	2 cut(s)	307, 834
BspPI	GGATC	3 cut(s)	24, 501, 711
BsrI	ACTGG	1 cut(s)	300
BssECI	CCNNGG	1 cut(s)	82
BssMI	GATC	9 cut(s)	16, 160, 320, 506, 610, 703, 721, 739, 847
Bst4CI	ACNGT	6 cut(s)	73, 118, 174, 572, 763, 869
Bst6I	CTCTTC	1 cut(s)	202
BstDEI	CTNAG	1 cut(s)	43
BstF5I	GGATG	2 cut(s)	553, 605
BstKTI	GATC	9 cut(s)	19, 163, 323, 509, 613, 706, 724, 742, 850
BstMAI	GTCTC	3 cut(s)	145, 199, 666
BstMBI	GATC	9 cut(s)	16, 160, 320, 506, 610, 703, 721, 739, 847
BstMWI	GCNNNNNNNGC	2 cut(s)	291, 371
BstSCI	CCNGG	2 cut(s)	82, 821
BstSFI	CTRYAG	1 cut(s)	451
BstV1I	GCAGC	3 cut(s)	26, 377, 815
BstX2I	RGATCY	6 cut(s)	16, 160, 320, 506, 721, 739
BstYI	RGATCY	6 cut(s)	16, 160, 320, 506, 721, 739
BsuI	GTATCC	2 cut(s)	440, 751
BsuRI	GGCC	1 cut(s)	5
BtgZI	GCGATG	1 cut(s)	786
BtrI	CACGTC	1 cut(s)	596
BtsCI	GGATG	2 cut(s)	553, 605
BtsIMutI	CAGTG	2 cut(s)	307, 874
CaiI	CAGNNNCTG	1 cut(s)	39
CseI	GACGC	1 cut(s)	274
Csp6I	GTAC	2 cut(s)	406, 462
CspCI	CAANNNNNGTGG	2 cut(s)	283, 318
CviAII	CATG	2 cut(s)	359, 396
CviQI	GTAC	2 cut(s)	406, 462
DdeI	CTNAG	1 cut(s)	43
DpnI	GATC	9 cut(s)	18, 162, 322, 508, 612, 705, 723, 741, 849
DpnII	GATC	9 cut(s)	16, 160, 320, 506, 610, 703, 721, 739, 847
DraI	TTTAAA	1 cut(s)	378
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	1 cut(s)	202
EarI	CTCTTC	1 cut(s)	202
EciI	GGCGGA	1 cut(s)	557
Eco57I	CTGAAG	1 cut(s)	657
EcoRI	GAATTC	1 cut(s)	797
Esp3I	CGTCTC	3 cut(s)	145, 199, 666
FaeI	CATG	2 cut(s)	362, 399
FaiI	YATR	9 cut(s)	76, 110, 212, 345, 360, 397, 436, 549, 895
FatI	CATG	2 cut(s)	358, 395
Fnu4HI	GCNGC	4 cut(s)	40, 366, 804, 873
FokI	GGATG	2 cut(s)	540, 592
Fsp4HI	GCNGC	4 cut(s)	40, 366, 804, 873
FspBI	CTAG	4 cut(s)	231, 291, 318, 710
GluI	GCNGC	4 cut(s)	40, 366, 804, 873
HaeIII	GGCC	1 cut(s)	5
HapII	CCGG	8 cut(s)	84, 279, 585, 665, 807, 822, 836, 885
HgaI	GACGC	1 cut(s)	274
Hin1II	CATG	2 cut(s)	362, 399
HindIII	AAGCTT	1 cut(s)	372
HinfI	GANTC	2 cut(s)	154, 185
HpaII	CCGG	8 cut(s)	84, 279, 585, 665, 807, 822, 836, 885
HphI	GGTGA	3 cut(s)	106, 423, 679
Hpy166II	GTNNAC	4 cut(s)	177, 431, 488, 865
Hpy188III	TCNNGA	3 cut(s)	158, 318, 725
Hpy8I	GTNNAC	4 cut(s)	177, 431, 488, 865
Hpy99I	CGWCG	4 cut(s)	11, 122, 637, 683
HpyAV	CCTTC	1 cut(s)	154
HpyCH4III	ACNGT	6 cut(s)	73, 118, 174, 572, 763, 869
HpyCH4IV	ACGT	2 cut(s)	253, 595
HpyF10VI	GCNNNNNNNGC	2 cut(s)	291, 371
HpyF3I	CTNAG	1 cut(s)	43
HpySE526I	ACGT	2 cut(s)	253, 595
Hsp92II	CATG	2 cut(s)	362, 399
Kzo9I	GATC	9 cut(s)	16, 160, 320, 506, 610, 703, 721, 739, 847
LmnI	GCTCC	5 cut(s)	311, 496, 544, 565, 838
Lsp1109I	GCAGC	3 cut(s)	26, 377, 815
MaeI	CTAG	4 cut(s)	231, 291, 318, 710
MaeII	ACGT	2 cut(s)	253, 595
MaeIII	GTNAC	3 cut(s)	286, 529, 667
MalI	GATC	9 cut(s)	18, 162, 322, 508, 612, 705, 723, 741, 849
MboI	GATC	9 cut(s)	16, 160, 320, 506, 610, 703, 721, 739, 847
MboII	GAAGA	8 cut(s)	189, 236, 267, 342, 529, 746, 749, 772
MflI	RGATCY	6 cut(s)	16, 160, 320, 506, 721, 739
MluCI	AATT	3 cut(s)	574, 683, 797
MmeI	TCCRAC	5 cut(s)	100, 534, 587, 656, 735
MnlI	CCTC	7 cut(s)	29, 267, 325, 437, 694, 787, 881
MseI	TTAA	1 cut(s)	377
MspI	CCGG	8 cut(s)	84, 279, 585, 665, 807, 822, 836, 885
MspR9I	CCNGG	2 cut(s)	84, 823
MwoI	GCNNNNNNNGC	2 cut(s)	291, 371
NciI	CCSGG	2 cut(s)	84, 823
NdeII	GATC	9 cut(s)	16, 160, 320, 506, 610, 703, 721, 739, 847
NlaIII	CATG	2 cut(s)	362, 399
NlaIV	GGNNCC	2 cut(s)	307, 834
NmeAIII	GCCGAG	1 cut(s)	809
NmuCI	GTSAC	3 cut(s)	286, 529, 667
PcsI	WCGNNNNNNNCGW	1 cut(s)	836
PfeI	GAWTC	2 cut(s)	154, 185
PfoI	TCCNGGA	1 cut(s)	821
PkrI	GCNGC	4 cut(s)	41, 367, 805, 874
PspN4I	GGNNCC	2 cut(s)	307, 834
PstNI	CAGNNNCTG	1 cut(s)	39
PsuI	RGATCY	6 cut(s)	16, 160, 320, 506, 721, 739
RsaI	GTAC	2 cut(s)	407, 463
RsaNI	GTAC	2 cut(s)	406, 462
SaqAI	TTAA	1 cut(s)	377
SatI	GCNGC	4 cut(s)	40, 366, 804, 873
Sau3AI	GATC	9 cut(s)	16, 160, 320, 506, 610, 703, 721, 739, 847
ScrFI	CCNGG	2 cut(s)	84, 823
SfcI	CTRYAG	1 cut(s)	451
Sse9I	AATT	3 cut(s)	574, 683, 797
SsiI	CCGC	3 cut(s)	542, 713, 873
SspMI	CTAG	4 cut(s)	231, 291, 318, 710
StyD4I	CCNGG	2 cut(s)	82, 821
TaaI	ACNGT	6 cut(s)	73, 118, 174, 572, 763, 869
TaiI	ACGT	2 cut(s)	256, 598
TaqI	TCGA	2 cut(s)	157, 783
TasI	AATT	3 cut(s)	574, 683, 797
TatI	WGTACW	1 cut(s)	461
TauI	GCSGC	1 cut(s)	875
TfiI	GAWTC	2 cut(s)	154, 185
Tru1I	TTAA	1 cut(s)	377
Tru9I	TTAA	1 cut(s)	377
TscAI	CASTG	2 cut(s)	307, 874
TseFI	GTSAC	3 cut(s)	286, 529, 667
TseI	GCWGC	3 cut(s)	39, 365, 803
Tsp45I	GTSAC	3 cut(s)	286, 529, 667
TspDTI	ATGAA	2 cut(s)	81, 645
TspGWI	ACGGA	2 cut(s)	423, 744
TspRI	CASTG	2 cut(s)	307, 874
XapI	RAATTY	2 cut(s)	683, 797
XbaI	TCTAGA	1 cut(s)	317
XspI	CTAG	4 cut(s)	231, 291, 318, 710

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.