RLG00000028008
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013949
Physical Location & Seq
Reverse (-)
17626774 .. 17627989
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028008

Sequence Viewer

Length: 990 bp
ATGGAAGACGATTGGGATCTCACCGCCGTGGTCAGAGGCTGCTCCACCGTCCCAACCACCAGCACCACCAACAAGCCAACCAACCCTTTCATCACAGACCAATTCCACACAAGCCCTCTCCTGTCCTCATTTGGTCAACAGGGTTTTGTCTCCAACAACACTACTCAAACCTTCTCTGTTCTTCCTCTTCCTCCAGATCCCATTACACCCCCAAGTACAATTGAGGAACTGCATGATCTTTACAAGCCATTCTTCCCCAAATCTCAACAAACCCCACCTCTTCTTTCTCCACCCCCACAAATCACACCACAGCCCACTTTCTCTTCCCCTCAACCGCTCCACAGACACCAACAACATCAGCCTAAGCTTTCTGCTACTAATCCCCAGCGCTCCAAAAAGAGAAAGAACCAGCTTAAGAAGGTGTGCCAAGTACCAGCGGAGTCTTTGTCTTCTGATGTGTGGGCATGGCGTAAATACGGACAGAAACCCATCAAGGGGTCACCATATCCGAGGGGTTACTACAGATGCAGCAGCTCAAAGGGTTGTATGGCCCGGAAGCAAGTGGAGCGGAACAGATCCGACCCGGGAATGTTCATAGTCACCTACACGGCGGAGCACAACCACCCTGCACCGACTCACCGCAACTCGCTTGCTGGGTCCACGCGTCACAAGCCTGTCTCTTCTCCCCAGTCGGGCTCCGTCACAGGCGGTGAAAATACCAAACCCAAGGGCTGCTCTCCGTCGCCGGCCACGTCGGCGGAGGAGGAGCTTGGTCGGAGCACAACAAGGATGGAAGCGAAAGAAGAAAAAAGTCCGATGGCGGATGAAGACGAAACAGAGTACTTTGGGGTGTGTGATACGATTGTGAGCGATGATTTCTTTGTGGGTTTGGAAGGTCTCGCCGGAGATTACTTTTCCGATCACTTTACGGCCAGCTTCGGCGGCCCGTGGAGTTCTGGTAATGCCGCCACTGCCGCCAGTAGTATCTGA

Protein Analysis

330

Amino Acids

36.03

Weight (kDa)

7.67

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 152 - 209 1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01250
fragaria_vesca FvH4_7g15350
malus_domestica MD01G1071300.v1.1 MD07G1131400.v1.1
prunus_persica Prupe.2G177400_v2.0.a1
pyrus_communis pycom01g10030
rosa_chinensis RchiOBHm_Chr1g0357671
rosa_laevigata RLG00000028008
rosa_multiflora Rmu_co8407323.1_g000001 Rmu_ssc0000425.1_g000001
rosa_roxburghii Rroxscaffold_4G00298610
rosa_rugosa Rorug01G0257600
rosa_samantha Rh1AG271600 Rh1BG238700 Rh1CG255500 Rh1DG267100
rosa_wichuraiana Rw1G024220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 337, 568
AccII CGCG 1 cut(s) 664
AclWI GGATC 3 cut(s) 24, 191, 570
AcoI YGGCCR 2 cut(s) 747, 930
AfaI GTAC 3 cut(s) 217, 432, 842
AfeI AGCGCT 1 cut(s) 389
AfiI CCNNNNNNNGG 3 cut(s) 494, 495, 692
AflII CTTAAG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 662
AjiI CACGTC 1 cut(s) 753
AleI CACNNNNGTG 1 cut(s) 26
AluBI AGCT 5 cut(s) 367, 412, 534, 769, 936
AluI AGCT 5 cut(s) 367, 412, 534, 769, 936
Alw21I GWGCWC 2 cut(s) 618, 782
Alw26I GTCTC 3 cut(s) 154, 682, 902
AlwI GGATC 3 cut(s) 24, 191, 570
AlwNI CAGNNNCTG 1 cut(s) 39
Ama87I CYCGRG 1 cut(s) 583
Aor51HI AGCGCT 1 cut(s) 389
AoxI GGCC 4 cut(s) 549, 747, 930, 943
ApeKI GCWGC 4 cut(s) 39, 528, 531, 732
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 3 cut(s) 550, 657, 944
AsuC2I CCSGG 3 cut(s) 553, 584, 585
AsuHPI GGTGA 5 cut(s) 13, 492, 592, 629, 722
AvaI CYCGRG 1 cut(s) 583
AvaII GGWCC 1 cut(s) 657
BaeI ACNNNNGTAYC 2 cut(s) 849, 882
BanII GRGCYC 1 cut(s) 698
BbsI GAAGAC 3 cut(s) 12, 441, 834
Bbv12I GWGCWC 2 cut(s) 618, 782
BbvI GCAGC 4 cut(s) 26, 540, 543, 719
BccI CCATC 3 cut(s) 497, 784, 811
BceAI ACGGC 3 cut(s) 11, 624, 945
BcnI CCSGG 3 cut(s) 553, 584, 585
BcoDI GTCTC 3 cut(s) 154, 682, 902
BfmI CTRYAG 1 cut(s) 520
BfoI RGCGCY 1 cut(s) 391
BfrI CTTAAG 1 cut(s) 413
BglI GCCNNNNNGGC 1 cut(s) 755
BisI GCNGC 7 cut(s) 40, 529, 532, 733, 943, 966, 975
BlsI GCNGC 7 cut(s) 41, 530, 533, 734, 944, 967, 976
BmcAI AGTACT 1 cut(s) 842
Bme1390I CCNGG 3 cut(s) 553, 584, 585
Bme18I GGWCC 1 cut(s) 657
BmeT110I CYCGRG 1 cut(s) 583
BmgBI CACGTC 1 cut(s) 753
BmgT120I GGNCC 3 cut(s) 550, 657, 944
BmiI GGNNCC 2 cut(s) 658, 697
BmrFI CCNGG 3 cut(s) 553, 584, 585
BmrI ACTGGG 1 cut(s) 682
BmsI GCATC 1 cut(s) 515
BmuI ACTGGG 1 cut(s) 682
BpiI GAAGAC 3 cut(s) 12, 441, 834
BpmI CTGGAG 1 cut(s) 177
Bpu10I CCTNAGC 1 cut(s) 363
BpuMI CCSGG 3 cut(s) 553, 584, 585
BsaBI GATNNNNATC 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 902
BsaJI CCNNGG 5 cut(s) 27, 509, 583, 726, 947
BsaXI ACNNNNNCTCC 4 cut(s) 431, 461, 943, 973
Bsc4I CCNNNNNNNGG 3 cut(s) 494, 495, 692
Bse118I RCCGGY 1 cut(s) 745
Bse1I ACTGG 2 cut(s) 688, 978
Bse8I GATNNNNATC 1 cut(s) 15
BseDI CCNNGG 5 cut(s) 27, 509, 583, 726, 947
BseGI GGATG 2 cut(s) 795, 829
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 3 cut(s) 494, 495, 692
BseNI ACTGG 2 cut(s) 688, 978
BseRI GAGGAG 2 cut(s) 776, 779
BseXI GCAGC 4 cut(s) 26, 540, 543, 719
BseYI CCCAGC 2 cut(s) 384, 653
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 664
BshFI GGCC 4 cut(s) 551, 749, 932, 945
BsiHKAI GWGCWC 2 cut(s) 618, 782
BsiHKCI CYCGRG 1 cut(s) 583
BsiSI CCGG 4 cut(s) 553, 584, 746, 903
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 3 cut(s) 494, 495, 692
BsmAI GTCTC 3 cut(s) 154, 682, 902
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 4 cut(s) 551, 749, 932, 945
Bso31I GGTCTC 1 cut(s) 902
BsoBI CYCGRG 1 cut(s) 583
Bsp1286I GDGCHC 3 cut(s) 618, 698, 782
Bsp143I GATC 5 cut(s) 16, 196, 235, 575, 919
BspANI GGCC 4 cut(s) 551, 749, 932, 945
BspFNI CGCG 1 cut(s) 664
BspLI GGNNCC 2 cut(s) 658, 697
BspPI GGATC 3 cut(s) 24, 191, 570
BspTI CTTAAG 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 902
BsrBI CCGCTC 2 cut(s) 337, 568
BsrFI RCCGGY 1 cut(s) 745
BsrI ACTGG 2 cut(s) 688, 978
BssAI RCCGGY 1 cut(s) 745
BssECI CCNNGG 5 cut(s) 27, 509, 583, 726, 947
BssMI GATC 5 cut(s) 16, 196, 235, 575, 919
BssT1I CCWWGG 1 cut(s) 726
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 4 cut(s) 192, 285, 328, 685
BstAFI CTTAAG 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 651, 747, 934
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 2 cut(s) 27, 947
BstEII GGTNACC 1 cut(s) 498
BstF5I GGATG 2 cut(s) 795, 829
BstFNI CGCG 1 cut(s) 664
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 5 cut(s) 19, 199, 238, 578, 922
BstMAI GTCTC 3 cut(s) 154, 682, 902
BstMBI GATC 5 cut(s) 16, 196, 235, 575, 919
BstMWI GCNNNNNNNGC 6 cut(s) 565, 670, 755, 942, 971, 974
BstPI GGTNACC 1 cut(s) 498
BstSCI CCNGG 3 cut(s) 551, 582, 583
BstSFI CTRYAG 1 cut(s) 520
BstUI CGCG 1 cut(s) 664
BstV1I GCAGC 4 cut(s) 26, 540, 543, 719
BstV2I GAAGAC 3 cut(s) 12, 441, 834
BstX2I RGATCY 3 cut(s) 16, 196, 575
BstYI RGATCY 3 cut(s) 16, 196, 575
BsuRI GGCC 4 cut(s) 551, 749, 932, 945
BtgI CCRYGG 2 cut(s) 27, 947
BtgZI GCGATG 1 cut(s) 885
BtrI CACGTC 1 cut(s) 753
BtsCI GGATG 2 cut(s) 795, 829
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 1 cut(s) 969
Cac8I GCNNGC 3 cut(s) 651, 747, 934
CaiI CAGNNNCTG 1 cut(s) 39
CfoI GCGC 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 745
Cfr13I GGNCC 3 cut(s) 550, 657, 944
Cfr9I CCCGGG 1 cut(s) 583
CseI GACGC 1 cut(s) 653
Csp6I GTAC 3 cut(s) 216, 431, 841
CviAII CATG 2 cut(s) 233, 465
CviQI GTAC 3 cut(s) 216, 431, 841
DdeI CTNAG 1 cut(s) 363
DpnI GATC 5 cut(s) 18, 198, 237, 577, 921
DpnII GATC 5 cut(s) 16, 196, 235, 575, 919
EaeI YGGCCR 2 cut(s) 747, 930
Eam1104I CTCTTC 4 cut(s) 192, 285, 328, 685
EarI CTCTTC 4 cut(s) 192, 285, 328, 685
EciI GGCGGA 3 cut(s) 626, 773, 836
Eco130I CCWWGG 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 698
Eco31I GGTCTC 1 cut(s) 902
Eco47I GGWCC 1 cut(s) 657
Eco47III AGCGCT 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 583
Eco91I GGTNACC 1 cut(s) 498
EcoO65I GGTNACC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 698
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 2 cut(s) 236, 468
FaiI YATR 5 cut(s) 234, 466, 505, 548, 596
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 35
FatI CATG 2 cut(s) 232, 464
Fnu4HI GCNGC 7 cut(s) 40, 529, 532, 733, 943, 966, 975
FokI GGATG 2 cut(s) 802, 836
FriOI GRGCYC 1 cut(s) 698
Fsp4HI GCNGC 7 cut(s) 40, 529, 532, 733, 943, 966, 975
GlaI GCGC 1 cut(s) 389
GluI GCNGC 7 cut(s) 40, 529, 532, 733, 943, 966, 975
GsaI CCCAGC 2 cut(s) 388, 657
GsuI CTGGAG 1 cut(s) 177
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 4 cut(s) 551, 749, 932, 945
HapII CCGG 4 cut(s) 553, 584, 746, 903
HgaI GACGC 1 cut(s) 653
HhaI GCGC 1 cut(s) 390
Hin1II CATG 2 cut(s) 236, 468
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HincII GTYRAC 1 cut(s) 137
HindII GTYRAC 1 cut(s) 137
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 2 cut(s) 440, 634
HpaII CCGG 4 cut(s) 553, 584, 746, 903
HphI GGTGA 5 cut(s) 13, 492, 592, 629, 722
Hpy166II GTNNAC 2 cut(s) 137, 660
Hpy188I TCNGA 8 cut(s) 35, 454, 510, 580, 777, 816, 919, 989
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 2 cut(s) 137, 660
Hpy99I CGWCG 2 cut(s) 745, 757
HpyAV CCTTC 3 cut(s) 181, 412, 887
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 752
HpyCH4V TGCA 3 cut(s) 232, 528, 629
HpyF10VI GCNNNNNNNGC 6 cut(s) 565, 670, 755, 942, 971, 974
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 752
Hsp92II CATG 2 cut(s) 236, 468
HspAI GCGC 1 cut(s) 388
KroI GCCGGC 1 cut(s) 745
KroNI GCCGGC 1 cut(s) 747
Kzo9I GATC 5 cut(s) 16, 196, 235, 575, 919
LmnI GCTCC 8 cut(s) 47, 342, 395, 565, 613, 701, 766, 777
Lsp1109I GCAGC 4 cut(s) 26, 540, 543, 719
LweI GCATC 1 cut(s) 515
MaeII ACGT 1 cut(s) 752
MaeIII GTNAC 5 cut(s) 498, 515, 598, 665, 700
MalI GATC 5 cut(s) 18, 198, 237, 577, 921
MbiI CCGCTC 2 cut(s) 337, 568
MboI GATC 5 cut(s) 16, 196, 235, 575, 919
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 3 cut(s) 16, 196, 575
MhlI GDGCHC 3 cut(s) 618, 698, 782
MluCI AATT 2 cut(s) 101, 219
MluI ACGCGT 1 cut(s) 662
MlyI GAGTC 2 cut(s) 449, 628
MmeI TCCRAC 3 cut(s) 177, 603, 755
MroNI GCCGGC 1 cut(s) 745
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 4 cut(s) 553, 584, 746, 903
MspR9I CCNGG 3 cut(s) 553, 584, 585
MunI CAATTG 1 cut(s) 219
MvnI CGCG 1 cut(s) 664
MwoI GCNNNNNNNGC 6 cut(s) 565, 670, 755, 942, 971, 974
NaeI GCCGGC 1 cut(s) 747
NciI CCSGG 3 cut(s) 553, 584, 585
NdeII GATC 5 cut(s) 16, 196, 235, 575, 919
NgoMIV GCCGGC 1 cut(s) 745
NlaIII CATG 2 cut(s) 236, 468
NlaIV GGNNCC 2 cut(s) 658, 697
NmuCI GTSAC 4 cut(s) 498, 598, 665, 700
OliI CACNNNNGTG 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 749
PdiI GCCGGC 1 cut(s) 747
PkrI GCNGC 7 cut(s) 41, 530, 533, 734, 944, 967, 976
PleI GAGTC 2 cut(s) 448, 628
PpsI GAGTC 2 cut(s) 448, 628
PspEI GGTNACC 1 cut(s) 498
PspFI CCCAGC 2 cut(s) 384, 653
PspN4I GGNNCC 2 cut(s) 658, 697
PspPI GGNCC 3 cut(s) 550, 657, 944
PstNI CAGNNNCTG 1 cut(s) 39
PsuI RGATCY 3 cut(s) 16, 196, 575
RsaI GTAC 3 cut(s) 217, 432, 842
RsaNI GTAC 3 cut(s) 216, 431, 841
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 7 cut(s) 40, 529, 532, 733, 943, 966, 975
Sau3AI GATC 5 cut(s) 16, 196, 235, 575, 919
Sau96I GGNCC 3 cut(s) 550, 657, 944
ScaI AGTACT 1 cut(s) 842
SchI GAGTC 2 cut(s) 449, 628
ScrFI CCNGG 3 cut(s) 553, 584, 585
SduI GDGCHC 3 cut(s) 618, 698, 782
SfaNI GCATC 1 cut(s) 515
SfcI CTRYAG 1 cut(s) 520
SinI GGWCC 1 cut(s) 657
SmaI CCCGGG 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 2 cut(s) 101, 219
StyD4I CCNGG 3 cut(s) 551, 582, 583
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 1 cut(s) 755
TasI AATT 2 cut(s) 101, 219
TatI WGTACW 2 cut(s) 215, 840
TauI GCSGC 3 cut(s) 945, 968, 977
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 1 cut(s) 976
TseFI GTSAC 4 cut(s) 498, 598, 665, 700
TseI GCWGC 4 cut(s) 39, 528, 531, 732
Tsp45I GTSAC 4 cut(s) 498, 598, 665, 700
TspDTI ATGAA 3 cut(s) 79, 583, 840
TspGWI ACGGA 3 cut(s) 492, 688, 729
TspMI CCCGGG 1 cut(s) 583
TspRI CASTG 1 cut(s) 976
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 657
XmaI CCCGGG 1 cut(s) 583
ZrmI AGTACT 1 cut(s) 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.