RchiOBHm_Chr1g0357671
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013949
Physical Location & Seq
Forward (+)
50013666 .. 50015099
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58289

Sequence Viewer

Length: 990 bp
ATGGAAGACGATTGGGATCTCACCGCCGTGGTCAGAGGTTGCTCCACCGTCCCAACCACCACCACCAACAAGCCAACCAACCCTTTCATCACAGATCACTTCCACACAAGCCCTCTCCTGTCTTCATTTGGTCAACAGGGTTATGTCTCCGACAACACTACTCAGACCTTCTCTGTTCTTCCTCTTCCTCCAGATCCCATTAAACCCACAAGTACAATTGAGGAACTGCATGATCTTTACAAGCCATTCTTCCCCAAATCTCAACAAACCCCTTCTCTTCTTTCTCCACCCCCACAAATCACACCACAGCCCAGTTTCTCTTCCCCTCAACCTCTCCACAGACACCAACAACATCAGCCGAAGCTTTCTGCTACTAATCCCCAGCGCTCCAAAAAGAGAAAGAACCAGCTTAAGAAGGTGTGCCAAGTACCAGCGGAGTCTTTGTCTTCTGATGTGTGGGCATGGCGTAAATACGGACAGAAACCCATCAAGGGCTCACCATATCCGAGAGGTTACTACAGATGCAGCAGCTCAAAGGGTTGTATGGCCCGGAAGCAAGTGGAGCGGAACAGATCCGACCCGGGAATGTTCATAGTCACCTACACGGCGGAGCACAACCATCCTGCACCGACTCACCGCAACTCGCTTGCTGGGTCCACGCGTCACAAGCCCGTCTCTTCTCCCCAGTCGGGCTCCGTCACAGGCGGTGACAATACCAAACCCAAGGGCTACTCTCCGTCGCCGGCCACGTCGGCGGAGGAGGAGCTTGGTCGGAGCACGACAAGGATGGAAGCGAAAGAAGAAAAACAGAGTCCTACGGCGGATGAAGACGAAACAGAGTACTTTGGGGTGTGTGATACGATTGTGAGCGATGATTTCTTTGTGGGTTTGGAAGGTCTCGCCGGAGATTACTTTTCCGATCACTTTACGGCCAGCTTCGGCGGCCCATGGAGTTCTGGTAATGCTGCCACCGCCGCCAGTAGTATCTGA

Protein Analysis

329

Amino Acids

36.12

Weight (kDa)

7.7

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 151 - 208 1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01250
fragaria_vesca FvH4_7g15350
malus_domestica MD01G1071300.v1.1 MD07G1131400.v1.1
prunus_persica Prupe.2G177400_v2.0.a1
pyrus_communis pycom01g10030
rosa_chinensis RchiOBHm_Chr1g0357671
rosa_laevigata RLG00000028008
rosa_multiflora Rmu_co8407323.1_g000001 Rmu_ssc0000425.1_g000001
rosa_roxburghii Rroxscaffold_4G00298610
rosa_rugosa Rorug01G0257600
rosa_samantha Rh1AG271600 Rh1BG238700 Rh1CG255500 Rh1DG267100
rosa_wichuraiana Rw1G024220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 565
AccII CGCG 1 cut(s) 661
AclWI GGATC 3 cut(s) 24, 188, 567
AcoI YGGCCR 2 cut(s) 744, 930
AfaI GTAC 3 cut(s) 214, 429, 842
AfeI AGCGCT 1 cut(s) 386
AfiI CCNNNNNNNGG 2 cut(s) 491, 689
AflII CTTAAG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 659
AjiI CACGTC 1 cut(s) 750
AleI CACNNNNGTG 1 cut(s) 26
AluBI AGCT 5 cut(s) 364, 409, 531, 766, 936
AluI AGCT 5 cut(s) 364, 409, 531, 766, 936
Alw21I GWGCWC 2 cut(s) 615, 779
Alw26I GTCTC 3 cut(s) 151, 679, 902
AlwI GGATC 3 cut(s) 24, 188, 567
Ama87I CYCGRG 1 cut(s) 580
Aor51HI AGCGCT 1 cut(s) 386
AoxI GGCC 4 cut(s) 546, 744, 930, 943
ApeKI GCWGC 3 cut(s) 525, 528, 965
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 3 cut(s) 547, 654, 944
AsuC2I CCSGG 3 cut(s) 550, 581, 582
AsuHPI GGTGA 5 cut(s) 13, 489, 589, 626, 719
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 1 cut(s) 654
BaeI ACNNNNGTAYC 2 cut(s) 849, 882
BanII GRGCYC 2 cut(s) 497, 695
BbsI GAAGAC 4 cut(s) 12, 114, 438, 834
Bbv12I GWGCWC 2 cut(s) 615, 779
BbvI GCAGC 3 cut(s) 537, 540, 952
BccI CCATC 3 cut(s) 494, 627, 781
BceAI ACGGC 4 cut(s) 11, 621, 834, 945
BcnI CCSGG 3 cut(s) 550, 581, 582
BcoDI GTCTC 3 cut(s) 151, 679, 902
BfmI CTRYAG 1 cut(s) 517
BfoI RGCGCY 1 cut(s) 388
BfrI CTTAAG 1 cut(s) 410
BglI GCCNNNNNGGC 1 cut(s) 752
BisI GCNGC 5 cut(s) 526, 529, 943, 966, 975
BlsI GCNGC 5 cut(s) 527, 530, 944, 967, 976
BmcAI AGTACT 1 cut(s) 842
Bme1390I CCNGG 3 cut(s) 550, 581, 582
Bme18I GGWCC 1 cut(s) 654
BmeT110I CYCGRG 1 cut(s) 580
BmgBI CACGTC 1 cut(s) 750
BmgT120I GGNCC 3 cut(s) 547, 654, 944
BmiI GGNNCC 2 cut(s) 655, 694
BmrFI CCNGG 3 cut(s) 550, 581, 582
BmrI ACTGGG 2 cut(s) 306, 679
BmsI GCATC 1 cut(s) 512
BmuI ACTGGG 2 cut(s) 306, 679
BpiI GAAGAC 4 cut(s) 12, 114, 438, 834
BpmI CTGGAG 1 cut(s) 174
BpuMI CCSGG 3 cut(s) 550, 581, 582
BsaBI GATNNNNATC 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 902
BsaJI CCNNGG 4 cut(s) 27, 580, 723, 947
BsaXI ACNNNNNCTCC 4 cut(s) 428, 458, 943, 973
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 689
Bse118I RCCGGY 1 cut(s) 742
Bse1I ACTGG 3 cut(s) 312, 685, 978
Bse8I GATNNNNATC 1 cut(s) 15
BseDI CCNNGG 4 cut(s) 27, 580, 723, 947
BseGI GGATG 3 cut(s) 619, 792, 829
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 2 cut(s) 491, 689
BseMII CTCAG 1 cut(s) 176
BseNI ACTGG 3 cut(s) 312, 685, 978
BseRI GAGGAG 2 cut(s) 773, 776
BseXI GCAGC 3 cut(s) 537, 540, 952
BseYI CCCAGC 2 cut(s) 381, 650
BsgI GTGCAG 1 cut(s) 609
Bsh1236I CGCG 1 cut(s) 661
BshFI GGCC 4 cut(s) 548, 746, 932, 945
BsiHKAI GWGCWC 2 cut(s) 615, 779
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 4 cut(s) 550, 581, 743, 903
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 491, 689
BsmAI GTCTC 3 cut(s) 151, 679, 902
BsmBI CGTCTC 1 cut(s) 679
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 4 cut(s) 548, 746, 932, 945
Bso31I GGTCTC 1 cut(s) 902
BsoBI CYCGRG 1 cut(s) 580
Bsp1286I GDGCHC 4 cut(s) 497, 615, 695, 779
Bsp143I GATC 6 cut(s) 16, 94, 193, 232, 572, 919
Bsp19I CCATGG 1 cut(s) 947
BspANI GGCC 4 cut(s) 548, 746, 932, 945
BspCNI CTCAG 1 cut(s) 175
BspFNI CGCG 1 cut(s) 661
BspLI GGNNCC 2 cut(s) 655, 694
BspPI GGATC 3 cut(s) 24, 188, 567
BspTI CTTAAG 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 902
BsrBI CCGCTC 1 cut(s) 565
BsrFI RCCGGY 1 cut(s) 742
BsrI ACTGG 3 cut(s) 312, 685, 978
BssAI RCCGGY 1 cut(s) 742
BssECI CCNNGG 4 cut(s) 27, 580, 723, 947
BssMI GATC 6 cut(s) 16, 94, 193, 232, 572, 919
BssT1I CCWWGG 2 cut(s) 723, 947
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 4 cut(s) 189, 282, 325, 682
BstAFI CTTAAG 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 648, 744, 934
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 2 cut(s) 27, 947
BstF5I GGATG 3 cut(s) 619, 792, 829
BstFNI CGCG 1 cut(s) 661
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 6 cut(s) 19, 97, 196, 235, 575, 922
BstMAI GTCTC 3 cut(s) 151, 679, 902
BstMBI GATC 6 cut(s) 16, 94, 193, 232, 572, 919
BstMWI GCNNNNNNNGC 6 cut(s) 562, 667, 752, 942, 971, 974
BstSCI CCNGG 3 cut(s) 548, 579, 580
BstSFI CTRYAG 1 cut(s) 517
BstUI CGCG 1 cut(s) 661
BstV1I GCAGC 3 cut(s) 537, 540, 952
BstV2I GAAGAC 4 cut(s) 12, 114, 438, 834
BstX2I RGATCY 3 cut(s) 16, 193, 572
BstYI RGATCY 3 cut(s) 16, 193, 572
BsuRI GGCC 4 cut(s) 548, 746, 932, 945
BtgI CCRYGG 2 cut(s) 27, 947
BtgZI GCGATG 1 cut(s) 885
BtrI CACGTC 1 cut(s) 750
BtsCI GGATG 3 cut(s) 619, 792, 829
Cac8I GCNNGC 3 cut(s) 648, 744, 934
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 1 cut(s) 742
Cfr13I GGNCC 3 cut(s) 547, 654, 944
Cfr9I CCCGGG 1 cut(s) 580
CseI GACGC 1 cut(s) 650
Csp6I GTAC 3 cut(s) 213, 428, 841
CviAII CATG 3 cut(s) 230, 462, 948
CviQI GTAC 3 cut(s) 213, 428, 841
DdeI CTNAG 1 cut(s) 162
DpnI GATC 6 cut(s) 18, 96, 195, 234, 574, 921
DpnII GATC 6 cut(s) 16, 94, 193, 232, 572, 919
EaeI YGGCCR 2 cut(s) 744, 930
Eam1104I CTCTTC 4 cut(s) 189, 282, 325, 682
EarI CTCTTC 4 cut(s) 189, 282, 325, 682
EciI GGCGGA 3 cut(s) 623, 770, 836
Eco130I CCWWGG 2 cut(s) 723, 947
Eco24I GRGCYC 2 cut(s) 497, 695
Eco31I GGTCTC 1 cut(s) 902
Eco47I GGWCC 1 cut(s) 654
Eco47III AGCGCT 1 cut(s) 386
Eco88I CYCGRG 1 cut(s) 580
EcoT14I CCWWGG 2 cut(s) 723, 947
EcoT38I GRGCYC 2 cut(s) 497, 695
ErhI CCWWGG 2 cut(s) 723, 947
Esp3I CGTCTC 1 cut(s) 679
FaeI CATG 3 cut(s) 233, 465, 951
FaiI YATR 7 cut(s) 144, 231, 463, 502, 545, 593, 949
FalI AAGNNNNNCTT 2 cut(s) 233, 265
FaqI GGGAC 1 cut(s) 35
FatI CATG 3 cut(s) 229, 461, 947
Fnu4HI GCNGC 5 cut(s) 526, 529, 943, 966, 975
FokI GGATG 3 cut(s) 606, 799, 836
FriOI GRGCYC 2 cut(s) 497, 695
Fsp4HI GCNGC 5 cut(s) 526, 529, 943, 966, 975
GlaI GCGC 1 cut(s) 386
GluI GCNGC 5 cut(s) 526, 529, 943, 966, 975
GsaI CCCAGC 2 cut(s) 385, 654
GsuI CTGGAG 1 cut(s) 174
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 4 cut(s) 548, 746, 932, 945
HapII CCGG 4 cut(s) 550, 581, 743, 903
HgaI GACGC 1 cut(s) 650
HhaI GCGC 1 cut(s) 387
Hin1II CATG 3 cut(s) 233, 465, 951
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HincII GTYRAC 1 cut(s) 134
HindII GTYRAC 1 cut(s) 134
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 3 cut(s) 437, 631, 811
HpaII CCGG 4 cut(s) 550, 581, 743, 903
HphI GGTGA 5 cut(s) 13, 489, 589, 626, 719
Hpy166II GTNNAC 2 cut(s) 134, 657
Hpy188I TCNGA 9 cut(s) 35, 151, 165, 451, 507, 577, 774, 919, 989
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 2 cut(s) 134, 657
Hpy99I CGWCG 2 cut(s) 742, 754
HpyAV CCTTC 4 cut(s) 178, 282, 409, 887
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 749
HpyCH4V TGCA 3 cut(s) 229, 525, 626
HpyF10VI GCNNNNNNNGC 6 cut(s) 562, 667, 752, 942, 971, 974
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 749
Hsp92II CATG 3 cut(s) 233, 465, 951
HspAI GCGC 1 cut(s) 385
KroI GCCGGC 1 cut(s) 742
KroNI GCCGGC 1 cut(s) 744
Kzo9I GATC 6 cut(s) 16, 94, 193, 232, 572, 919
LmnI GCTCC 7 cut(s) 47, 392, 562, 610, 698, 763, 774
Lsp1109I GCAGC 3 cut(s) 537, 540, 952
LweI GCATC 1 cut(s) 512
MaeII ACGT 1 cut(s) 749
MaeIII GTNAC 5 cut(s) 512, 595, 662, 697, 707
MalI GATC 6 cut(s) 18, 96, 195, 234, 574, 921
MbiI CCGCTC 1 cut(s) 565
MboI GATC 6 cut(s) 16, 94, 193, 232, 572, 919
MfeI CAATTG 1 cut(s) 216
MflI RGATCY 3 cut(s) 16, 193, 572
MhlI GDGCHC 4 cut(s) 497, 615, 695, 779
MluCI AATT 1 cut(s) 216
MluI ACGCGT 1 cut(s) 659
MlyI GAGTC 3 cut(s) 446, 625, 820
MmeI TCCRAC 3 cut(s) 174, 600, 752
MroNI GCCGGC 1 cut(s) 742
MseI TTAA 2 cut(s) 201, 411
MslI CAYNNNNRTG 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 4 cut(s) 550, 581, 743, 903
MspR9I CCNGG 3 cut(s) 550, 581, 582
MunI CAATTG 1 cut(s) 216
MvnI CGCG 1 cut(s) 661
MwoI GCNNNNNNNGC 6 cut(s) 562, 667, 752, 942, 971, 974
NaeI GCCGGC 1 cut(s) 744
NciI CCSGG 3 cut(s) 550, 581, 582
NcoI CCATGG 1 cut(s) 947
NdeII GATC 6 cut(s) 16, 94, 193, 232, 572, 919
NgoMIV GCCGGC 1 cut(s) 742
NlaIII CATG 3 cut(s) 233, 465, 951
NlaIV GGNNCC 2 cut(s) 655, 694
NmuCI GTSAC 4 cut(s) 595, 662, 697, 707
OliI CACNNNNGTG 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 746
PdiI GCCGGC 1 cut(s) 744
PkrI GCNGC 5 cut(s) 527, 530, 944, 967, 976
PleI GAGTC 3 cut(s) 445, 625, 819
PpsI GAGTC 3 cut(s) 445, 625, 819
PspFI CCCAGC 2 cut(s) 381, 650
PspN4I GGNNCC 2 cut(s) 655, 694
PspPI GGNCC 3 cut(s) 547, 654, 944
PsuI RGATCY 3 cut(s) 16, 193, 572
RsaI GTAC 3 cut(s) 214, 429, 842
RsaNI GTAC 3 cut(s) 213, 428, 841
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 2 cut(s) 201, 411
SatI GCNGC 5 cut(s) 526, 529, 943, 966, 975
Sau3AI GATC 6 cut(s) 16, 94, 193, 232, 572, 919
Sau96I GGNCC 3 cut(s) 547, 654, 944
ScaI AGTACT 1 cut(s) 842
SchI GAGTC 3 cut(s) 446, 625, 820
ScrFI CCNGG 3 cut(s) 550, 581, 582
SduI GDGCHC 4 cut(s) 497, 615, 695, 779
SfaNI GCATC 1 cut(s) 512
SfcI CTRYAG 1 cut(s) 517
SinI GGWCC 1 cut(s) 654
SmaI CCCGGG 1 cut(s) 582
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 1 cut(s) 216
StyD4I CCNGG 3 cut(s) 548, 579, 580
StyI CCWWGG 2 cut(s) 723, 947
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 1 cut(s) 752
TasI AATT 1 cut(s) 216
TatI WGTACW 2 cut(s) 212, 840
TauI GCSGC 2 cut(s) 945, 977
Tru1I TTAA 2 cut(s) 201, 411
Tru9I TTAA 2 cut(s) 201, 411
TseFI GTSAC 4 cut(s) 595, 662, 697, 707
TseI GCWGC 3 cut(s) 525, 528, 965
Tsp45I GTSAC 4 cut(s) 595, 662, 697, 707
TspDTI ATGAA 4 cut(s) 76, 114, 580, 840
TspGWI ACGGA 3 cut(s) 489, 685, 726
TspMI CCCGGG 1 cut(s) 580
Vha464I CTTAAG 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 654
XmaI CCCGGG 1 cut(s) 580
ZrmI AGTACT 1 cut(s) 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.