pycom01g10030
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013949
Physical Location & Seq
Forward (+)
10884967 .. 10886239
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g10030.1

Sequence Viewer

Length: 1041 bp
ATGGAGGACGATTGGGATCTTCAAGCTGTGGTCCGAGGCTGCTCCACCGCCACCGCCACTACCACCACATCATCAACCAGAACTCCCTCCACCGGTGCCACCGCAAGTTTCAACATCTCCGGCTTCCATTCAAACCCAGCAGCTGCTTCCTTCTCTTCATTTGGTCCAACTTCCCCACAACAGCAACTCCTCTTCTCTCCCCCACTTCCAGATCCCATCATTAAACCCAGAAATGCCATTGAAGAATTGCATGAGCTTTACAAGCCTTTCTTCCCCAAATCTCAGCCTTCCCTCTCCTCCCCACAAATCGCACCACTCACTCTCTCTCCTCTGACCACACCCAAAGATCCAAGGCATCCCATACAGTACCACCAACAAACCCAGCACCAACAACAACAAACCCAGCACTCCAAGCCATCATCTTCCACCACCACCGCACGATCCAAAAAAAGAAAGAACCAGCTTAAAAAAGTGTGCCAAGTTCCGGCGGAGGGTCTCTCTTCAGACGTATGGGCATGGCGGAAGTACGGCCAAAAACCCATCAAGGGTTCCCCATATCCGAGGGGATATTACAGATGCAGCAGCTCGAAGGGTTGTATGGCCCGGAAACAAGTGGAGCGGAACAGATCCGACCCGAATATGTTCATAGTCACCTACACGGGGGAGCACAACCACCCGGCCCCAACTCACCGCAACTCGCTCGCCGGCTCCACTCGCCAGAAGCCCTTCTCGCCACAAACCGCCACGCGGGGTGACTCTGCGAAACCCGCTTCTCCGACAACCTCGGTCTCCGCGGACGAGGAGACTGTCGTTGCACCTCAGAGTACGACCGTGGAAAGCAGCTGCAAGGAAGAGAAGGGGAGTCCTTTGATTACTGACGAGGAGGACGAGTTGTTGGGGATGTGCGACTCGGTCGTGAGTGACGATTTCTTTGTTGGGCTGGACGGTCTGGCCGGAGATTACTTTTCCGATCACTCGACGGGGAGCTTCGGCATGCCTTGGATTTCTAGTAGTGCTGCCACCGCCGCTGGTAGTATCTGA

Protein Analysis

347

Amino Acids

37.5

Weight (kDa)

8.16

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 169 - 226 5.2e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01250
fragaria_vesca FvH4_7g15350
malus_domestica MD01G1071300.v1.1 MD07G1131400.v1.1
prunus_persica Prupe.2G177400_v2.0.a1
pyrus_communis pycom01g10030
rosa_chinensis RchiOBHm_Chr1g0357671
rosa_laevigata RLG00000028008
rosa_multiflora Rmu_co8407323.1_g000001 Rmu_ssc0000425.1_g000001
rosa_roxburghii Rroxscaffold_4G00298610
rosa_rugosa Rorug01G0257600
rosa_samantha Rh1AG271600 Rh1BG238700 Rh1CG255500 Rh1DG267100
rosa_wichuraiana Rw1G024220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 619
AccII CGCG 2 cut(s) 748, 794
AclWI GGATC 5 cut(s) 24, 206, 341, 435, 621
AcoI YGGCCR 2 cut(s) 529, 951
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 3 cut(s) 368, 527, 826
AfiI CCNNNNNNNGG 6 cut(s) 92, 484, 491, 545, 660, 747
AgeI ACCGGT 1 cut(s) 92
AgsI TTSAA 4 cut(s) 23, 112, 132, 242
AluBI AGCT 7 cut(s) 26, 143, 256, 463, 585, 843, 987
AluI AGCT 7 cut(s) 26, 143, 256, 463, 585, 843, 987
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 3 cut(s) 500, 793, 797
AlwI GGATC 5 cut(s) 24, 206, 341, 435, 621
AlwNI CAGNNNCTG 1 cut(s) 143
AoxI GGCC 4 cut(s) 529, 600, 678, 951
ApeKI GCWGC 8 cut(s) 39, 140, 143, 579, 582, 840, 843, 1016
ArsI GACNNNNNNTTYG 2 cut(s) 914, 946
AsiGI ACCGGT 1 cut(s) 92
Asp700I GAANNNNTTC 2 cut(s) 641, 725
AspS9I GGNCC 4 cut(s) 31, 164, 601, 679
AsuC2I CCSGG 2 cut(s) 604, 677
AsuHPI GGTGA 3 cut(s) 643, 680, 764
AvaII GGWCC 2 cut(s) 31, 164
BanI GGYRCC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 8 cut(s) 26, 130, 152, 591, 594, 830, 852, 1003
BccI CCATC 3 cut(s) 224, 424, 548
BceAI ACGGC 1 cut(s) 544
BcgI CGANNNNNNTGC 2 cut(s) 682, 716
BcnI CCSGG 2 cut(s) 604, 677
BcoDI GTCTC 3 cut(s) 500, 793, 797
BfaI CTAG 1 cut(s) 1008
BisI GCNGC 9 cut(s) 40, 141, 144, 580, 583, 841, 844, 1017, 1026
BlsI GCNGC 9 cut(s) 41, 142, 145, 581, 584, 842, 845, 1018, 1027
Bme1390I CCNGG 2 cut(s) 604, 677
Bme18I GGWCC 2 cut(s) 31, 164
BmgT120I GGNCC 4 cut(s) 31, 164, 601, 679
BmiI GGNNCC 4 cut(s) 97, 550, 681, 709
BmrFI CCNGG 2 cut(s) 604, 677
BmsI GCATC 2 cut(s) 364, 566
BplI GAGNNNNNCTC 2 cut(s) 482, 514
BpuMI CCSGG 2 cut(s) 604, 677
BsaBI GATNNNNATC 1 cut(s) 15
BsaI GGTCTC 2 cut(s) 500, 793
BsaJI CCNNGG 7 cut(s) 34, 350, 560, 783, 792, 831, 998
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 8 cut(s) 67, 97, 171, 201, 310, 340, 875, 905
Bsc4I CCNNNNNNNGG 6 cut(s) 92, 484, 491, 545, 660, 747
Bse118I RCCGGY 2 cut(s) 92, 704
Bse8I GATNNNNATC 1 cut(s) 15
BseDI CCNNGG 7 cut(s) 34, 350, 560, 783, 792, 831, 998
BseGI GGATG 2 cut(s) 355, 906
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 6 cut(s) 92, 484, 491, 545, 660, 747
BseMII CTCAG 2 cut(s) 296, 833
BseRI GAGGAG 5 cut(s) 179, 286, 318, 815, 896
BseXI GCAGC 8 cut(s) 26, 130, 152, 591, 594, 830, 852, 1003
BseYI CCCAGC 3 cut(s) 136, 381, 402
Bsh1236I CGCG 2 cut(s) 748, 794
Bsh1285I CGRYCG 2 cut(s) 831, 915
BshFI GGCC 4 cut(s) 531, 602, 680, 953
BshNI GGYRCC 1 cut(s) 95
BshTI ACCGGT 1 cut(s) 92
BsiEI CGRYCG 2 cut(s) 831, 915
BsiHKAI GWGCWC 1 cut(s) 669
BsiSI CCGG 7 cut(s) 93, 120, 485, 604, 677, 705, 954
BslI CCNNNNNNNGG 6 cut(s) 92, 484, 491, 545, 660, 747
BsmAI GTCTC 3 cut(s) 500, 793, 797
BsnI GGCC 4 cut(s) 531, 602, 680, 953
Bso31I GGTCTC 2 cut(s) 500, 793
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 6 cut(s) 16, 211, 346, 440, 626, 970
BspANI GGCC 4 cut(s) 531, 602, 680, 953
BspCNI CTCAG 2 cut(s) 295, 832
BspFNI CGCG 2 cut(s) 748, 794
BspLI GGNNCC 4 cut(s) 97, 550, 681, 709
BspPI GGATC 5 cut(s) 24, 206, 341, 435, 621
BspT107I GGYRCC 1 cut(s) 95
BspTNI GGTCTC 2 cut(s) 500, 793
BsrBI CCGCTC 1 cut(s) 619
BsrFI RCCGGY 2 cut(s) 92, 704
BssAI RCCGGY 2 cut(s) 92, 704
BssECI CCNNGG 7 cut(s) 34, 350, 560, 783, 792, 831, 998
BssMI GATC 6 cut(s) 16, 211, 346, 440, 626, 970
BssT1I CCWWGG 2 cut(s) 350, 998
Bst4CI ACNGT 4 cut(s) 366, 808, 832, 947
Bst6I CTCTTC 4 cut(s) 160, 197, 505, 846
BstC8I GCNNGC 3 cut(s) 702, 706, 995
BstDEI CTNAG 2 cut(s) 282, 819
BstDSI CCRYGG 2 cut(s) 792, 831
BstF5I GGATG 2 cut(s) 355, 906
BstFNI CGCG 2 cut(s) 748, 794
BstKTI GATC 6 cut(s) 19, 214, 349, 443, 629, 973
BstMAI GTCTC 3 cut(s) 500, 793, 797
BstMBI GATC 6 cut(s) 16, 211, 346, 440, 626, 970
BstMCI CGRYCG 2 cut(s) 831, 915
BstMWI GCNNNNNNNGC 7 cut(s) 262, 412, 714, 730, 767, 1022, 1025
BstNSI RCATGY 1 cut(s) 997
BstSCI CCNGG 2 cut(s) 602, 675
BstUI CGCG 2 cut(s) 748, 794
BstV1I GCAGC 8 cut(s) 26, 130, 152, 591, 594, 830, 852, 1003
BstX2I RGATCY 4 cut(s) 16, 211, 346, 626
BstYI RGATCY 4 cut(s) 16, 211, 346, 626
BsuRI GGCC 4 cut(s) 531, 602, 680, 953
BtgI CCRYGG 2 cut(s) 792, 831
BtsCI GGATG 2 cut(s) 355, 906
Cac8I GCNNGC 3 cut(s) 702, 706, 995
CaiI CAGNNNCTG 1 cut(s) 143
Cfr10I RCCGGY 2 cut(s) 92, 704
Cfr13I GGNCC 4 cut(s) 31, 164, 601, 679
Cfr42I CCGCGG 1 cut(s) 795
Csp6I GTAC 3 cut(s) 367, 526, 825
CspAI ACCGGT 1 cut(s) 92
CviAII CATG 3 cut(s) 251, 516, 994
CviQI GTAC 3 cut(s) 367, 526, 825
DdeI CTNAG 2 cut(s) 282, 819
DpnI GATC 6 cut(s) 18, 213, 348, 442, 628, 972
DpnII GATC 6 cut(s) 16, 211, 346, 440, 626, 970
EaeI YGGCCR 2 cut(s) 529, 951
Eam1104I CTCTTC 4 cut(s) 160, 197, 505, 846
EarI CTCTTC 4 cut(s) 160, 197, 505, 846
EciI GGCGGA 2 cut(s) 503, 535
Eco130I CCWWGG 2 cut(s) 350, 998
Eco31I GGTCTC 2 cut(s) 500, 793
Eco47I GGWCC 2 cut(s) 31, 164
Eco57I CTGAAG 1 cut(s) 486
EcoT14I CCWWGG 2 cut(s) 350, 998
ErhI CCWWGG 2 cut(s) 350, 998
FaeI CATG 3 cut(s) 254, 519, 997
FaiI YATR 9 cut(s) 252, 362, 511, 517, 556, 599, 641, 647, 995
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FatI CATG 3 cut(s) 250, 515, 993
FauI CCCGC 2 cut(s) 741, 775
Fnu4HI GCNGC 9 cut(s) 40, 141, 144, 580, 583, 841, 844, 1017, 1026
FokI GGATG 2 cut(s) 342, 913
Fsp4HI GCNGC 9 cut(s) 40, 141, 144, 580, 583, 841, 844, 1017, 1026
FspBI CTAG 1 cut(s) 1008
GluI GCNGC 9 cut(s) 40, 141, 144, 580, 583, 841, 844, 1017, 1026
GsaI CCCAGC 3 cut(s) 140, 385, 406
HaeIII GGCC 4 cut(s) 531, 602, 680, 953
HapII CCGG 7 cut(s) 93, 120, 485, 604, 677, 705, 954
Hin1II CATG 3 cut(s) 254, 519, 997
HinfI GANTC 3 cut(s) 755, 862, 908
HpaII CCGG 7 cut(s) 93, 120, 485, 604, 677, 705, 954
HphI GGTGA 3 cut(s) 643, 680, 764
Hpy188I TCNGA 9 cut(s) 35, 333, 505, 561, 631, 777, 822, 970, 1040
Hpy188III TCNNGA 2 cut(s) 209, 916
Hpy99I CGWCG 1 cut(s) 982
HpyAV CCTTC 5 cut(s) 160, 297, 583, 736, 850
HpyCH4III ACNGT 4 cut(s) 366, 808, 832, 947
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 4 cut(s) 250, 579, 815, 846
HpyF10VI GCNNNNNNNGC 7 cut(s) 262, 412, 714, 730, 767, 1022, 1025
HpyF3I CTNAG 2 cut(s) 282, 819
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 3 cut(s) 254, 519, 997
KroI GCCGGC 1 cut(s) 704
KroNI GCCGGC 1 cut(s) 706
KspI CCGCGG 1 cut(s) 795
Kzo9I GATC 6 cut(s) 16, 211, 346, 440, 626, 970
LmnI GCTCC 5 cut(s) 47, 616, 664, 713, 984
Lsp1109I GCAGC 8 cut(s) 26, 130, 152, 591, 594, 830, 852, 1003
LweI GCATC 2 cut(s) 364, 566
MaeI CTAG 1 cut(s) 1008
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 3 cut(s) 649, 752, 920
MalI GATC 6 cut(s) 18, 213, 348, 442, 628, 972
MbiI CCGCTC 1 cut(s) 619
MboI GATC 6 cut(s) 16, 211, 346, 440, 626, 970
MboII GAAGA 8 cut(s) 11, 147, 184, 254, 262, 414, 492, 863
MflI RGATCY 4 cut(s) 16, 211, 346, 626
MhlI GDGCHC 1 cut(s) 669
MluCI AATT 1 cut(s) 245
MlyI GAGTC 3 cut(s) 749, 871, 902
MmeI TCCRAC 3 cut(s) 191, 654, 800
MroNI GCCGGC 1 cut(s) 704
MroXI GAANNNNTTC 2 cut(s) 641, 725
MseI TTAA 2 cut(s) 222, 465
MspA1I CMGCKG 4 cut(s) 143, 794, 843, 1028
MspI CCGG 7 cut(s) 93, 120, 485, 604, 677, 705, 954
MspR9I CCNGG 2 cut(s) 604, 677
MvnI CGCG 2 cut(s) 748, 794
MwoI GCNNNNNNNGC 7 cut(s) 262, 412, 714, 730, 767, 1022, 1025
NaeI GCCGGC 1 cut(s) 706
NciI CCSGG 2 cut(s) 604, 677
NdeII GATC 6 cut(s) 16, 211, 346, 440, 626, 970
NgoMIV GCCGGC 1 cut(s) 704
NlaIII CATG 3 cut(s) 254, 519, 997
NlaIV GGNNCC 4 cut(s) 97, 550, 681, 709
NmuCI GTSAC 3 cut(s) 649, 752, 920
NspI RCATGY 1 cut(s) 997
PaeI GCATGC 1 cut(s) 997
PcsI WCGNNNNNNNCGW 2 cut(s) 885, 921
PdiI GCCGGC 1 cut(s) 706
PdmI GAANNNNTTC 2 cut(s) 641, 725
PflFI GACNNNGTC 1 cut(s) 911
PinAI ACCGGT 1 cut(s) 92
PkrI GCNGC 9 cut(s) 41, 142, 145, 581, 584, 842, 845, 1018, 1027
PleI GAGTC 3 cut(s) 749, 870, 902
PpsI GAGTC 3 cut(s) 749, 870, 902
PspFI CCCAGC 3 cut(s) 136, 381, 402
PspN4I GGNNCC 4 cut(s) 97, 550, 681, 709
PspPI GGNCC 4 cut(s) 31, 164, 601, 679
PstNI CAGNNNCTG 1 cut(s) 143
PsuI RGATCY 4 cut(s) 16, 211, 346, 626
PsyI GACNNNGTC 1 cut(s) 911
PvuII CAGCTG 2 cut(s) 143, 843
RsaI GTAC 3 cut(s) 368, 527, 826
RsaNI GTAC 3 cut(s) 367, 526, 825
SacII CCGCGG 1 cut(s) 795
SaqAI TTAA 2 cut(s) 222, 465
SatI GCNGC 9 cut(s) 40, 141, 144, 580, 583, 841, 844, 1017, 1026
Sau3AI GATC 6 cut(s) 16, 211, 346, 440, 626, 970
Sau96I GGNCC 4 cut(s) 31, 164, 601, 679
SchI GAGTC 3 cut(s) 749, 871, 902
ScrFI CCNGG 2 cut(s) 604, 677
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 2 cut(s) 364, 566
Sfr303I CCGCGG 1 cut(s) 795
SgrAI CRCCGGYG 1 cut(s) 92
SgrBI CCGCGG 1 cut(s) 795
SinI GGWCC 2 cut(s) 31, 164
SphI GCATGC 1 cut(s) 997
Sse9I AATT 1 cut(s) 245
SspMI CTAG 1 cut(s) 1008
StyD4I CCNGG 2 cut(s) 602, 675
StyI CCWWGG 2 cut(s) 350, 998
TaaI ACNGT 4 cut(s) 366, 808, 832, 947
TaiI ACGT 1 cut(s) 510
TaqI TCGA 2 cut(s) 587, 977
TaqII GACCGA 2 cut(s) 775, 901
TasI AATT 1 cut(s) 245
TauI GCSGC 1 cut(s) 1028
Tru1I TTAA 2 cut(s) 222, 465
Tru9I TTAA 2 cut(s) 222, 465
TseFI GTSAC 3 cut(s) 649, 752, 920
TseI GCWGC 8 cut(s) 39, 140, 143, 579, 582, 840, 843, 1016
Tsp45I GTSAC 3 cut(s) 649, 752, 920
TspDTI ATGAA 2 cut(s) 147, 634
Tth111I GACNNNGTC 1 cut(s) 911
VpaK11BI GGWCC 2 cut(s) 31, 164
XceI RCATGY 1 cut(s) 997
XmnI GAANNNNTTC 2 cut(s) 641, 725
XspI CTAG 1 cut(s) 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.