Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013949

Physical Location & Seq

Genomic Coordinates Forward (+)

17602924 .. 17604669

Gene Structure 1746 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1071300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1050 bp

ATGGAGGACGATTGGGATCTTCAAGCGGTGGTCCGAGGCTGCTCCACCGCCACCGCCACTACCACCACATCATCAACCAGAACTCCCTCCACCGCTGCCACCGCAAGTTTCAACATCTCCGGCTTCCATTCAAACCCAGCAGCTGCTTCCTTCTCTTCATTTGGTCCAACTTCCCCACAACAGCAACTCCTCTTCTCTCTCCCACTTCCAGATCCCATCATTAAACCCAGAAATGCCATTGAAGAATTGCATGAGCTTTACAAGCCTTTCTTCCCCAAATCTCAGCCTTCCCTCTCCTCCCCACAAATCACACCACCCACTCTCTCTCCTTTGACTTCTCTCTCTCCTCTGACCACACCCAAAGATCCAAGGCATCCCATACACCAACAACAACAAACCCAGCACTCCAAGCCATCATCTTCCACCACCACCACAACCACCACCGCACGATCCAAAAAAAGAAAGAACCAGCTTAAAAAAGTGTGCCAAGTTCCGGTGGAGGCCCTCTCTTCAGACATATGGGCATGGCGGAAGTACGGCCAAAAACCCATCAAGGGTTCCCCATATCCGAGGGGATATTACAGATGCAGCAGCTCGAAGGGTTGTATGGCCCGGAAGCAAGTGGAGCGGAACAGATCCGACCCGAATATGTTCATAGTCACCTACACGGGGGAGCACAACCACCCGGCCCCAACTCACCGCAACTCGCTCGCCGGCTCCACGCGCCAGAAGCCCTTCTCGCCACAAACCGCCACGCGGGGTGACTCTGCGAAACCCGCTTCTCCGACAACCTCGGCCTCCGCGGACGAGGAGCCTGTCGTTGCACCTCAGAGTACGACCGTGGAAAGCAGCTGCAAGGAAGAGAAGGGGAGTCCTTTGATTACCGACGAGGAAGACGAGTTGTTGGGGATGTGCGACTCGGTCGTGAGTGACGATTTCTTTGTTGGGCTGGACGGTCTAGCCGGAGATTACTTTTCCGATCACTCGACGGGGAGCTTCGGCATGCCTTGGATTTCTAGTAGTGCTGCCACTGCCGCTGGTAGTATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0009889 GO:0010033 GO:0010150 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042221 GO:0042493 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090693 GO:0099402 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

WRKY

Protein Analysis

350

Amino Acids

37.65

Weight (kDa)

8.16

Isoelectric Point (pI)

55.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	172 - 229	5.5e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01250
fragaria_vesca	FvH4_7g15350
malus_domestica	MD01G1071300.v1.1 MD07G1131400.v1.1
prunus_persica	Prupe.2G177400_v2.0.a1
pyrus_communis	pycom01g10030
rosa_chinensis	RchiOBHm_Chr1g0357671
rosa_laevigata	RLG00000028008
rosa_multiflora	Rmu_co8407323.1_g000001 Rmu_ssc0000425.1_g000001
rosa_roxburghii	Rroxscaffold_4G00298610
rosa_rugosa	Rorug01G0257600
rosa_samantha	Rh1AG271600 Rh1BG238700 Rh1CG255500 Rh1DG267100
rosa_wichuraiana	Rw1G024220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	628
AccII	CGCG	3 cut(s)	724, 757, 803
AclWI	GGATC	5 cut(s)	24, 206, 359, 444, 630
AcoI	YGGCCR	1 cut(s)	538
AcuI	CTGAAG	1 cut(s)	495
AfaI	GTAC	2 cut(s)	536, 835
AfiI	CCNNNNNNNGG	4 cut(s)	493, 554, 669, 756
AgsI	TTSAA	4 cut(s)	23, 112, 132, 242
AluBI	AGCT	6 cut(s)	143, 256, 472, 594, 852, 996
AluI	AGCT	6 cut(s)	143, 256, 472, 594, 852, 996
Alw21I	GWGCWC	1 cut(s)	678
AlwI	GGATC	5 cut(s)	24, 206, 359, 444, 630
AlwNI	CAGNNNCTG	1 cut(s)	143
AoxI	GGCC	5 cut(s)	501, 538, 609, 687, 795
ApeKI	GCWGC	9 cut(s)	39, 95, 140, 143, 588, 591, 849, 852, 1025
ArsI	GACNNNNNNTTYG	2 cut(s)	923, 955
Asp700I	GAANNNNTTC	2 cut(s)	650, 734
AspLEI	GCGC	1 cut(s)	726
AspS9I	GGNCC	5 cut(s)	31, 164, 502, 610, 688
AsuC2I	CCSGG	2 cut(s)	613, 686
AsuHPI	GGTGA	3 cut(s)	652, 689, 773
AvaII	GGWCC	2 cut(s)	31, 164
BbsI	GAAGAC	1 cut(s)	900
Bbv12I	GWGCWC	1 cut(s)	678
BbvI	GCAGC	9 cut(s)	26, 82, 130, 152, 600, 603, 839, 861, 1012
BccI	CCATC	3 cut(s)	224, 421, 557
BceAI	ACGGC	1 cut(s)	553
BcgI	CGANNNNNNTGC	2 cut(s)	691, 725
BcnI	CCSGG	2 cut(s)	613, 686
BfaI	CTAG	2 cut(s)	959, 1017
Bme1390I	CCNGG	2 cut(s)	613, 686
Bme18I	GGWCC	2 cut(s)	31, 164
BmgT120I	GGNCC	5 cut(s)	31, 164, 502, 610, 688
BmiI	GGNNCC	4 cut(s)	559, 690, 718, 813
BmrFI	CCNGG	2 cut(s)	613, 686
BmsI	GCATC	2 cut(s)	382, 575
BpiI	GAAGAC	1 cut(s)	900
BplI	GAGNNNNNCTC	2 cut(s)	491, 523
BpuMI	CCSGG	2 cut(s)	613, 686
BsaBI	GATNNNNATC	1 cut(s)	15
BsaJI	CCNNGG	7 cut(s)	34, 368, 569, 792, 801, 840, 1007
BsaWI	WCCGGW	1 cut(s)	493
BsaXI	ACNNNNNCTCC	6 cut(s)	67, 97, 171, 201, 310, 340
Bsc4I	CCNNNNNNNGG	4 cut(s)	493, 554, 669, 756
Bse118I	RCCGGY	1 cut(s)	713
Bse8I	GATNNNNATC	1 cut(s)	15
BseDI	CCNNGG	7 cut(s)	34, 368, 569, 792, 801, 840, 1007
BseGI	GGATG	2 cut(s)	373, 915
BseJI	GATNNNNATC	1 cut(s)	15
BseLI	CCNNNNNNNGG	4 cut(s)	493, 554, 669, 756
BseMII	CTCAG	2 cut(s)	296, 842
BseRI	GAGGAG	4 cut(s)	179, 286, 336, 824
BseXI	GCAGC	9 cut(s)	26, 82, 130, 152, 600, 603, 839, 861, 1012
BseYI	CCCAGC	2 cut(s)	136, 399
Bsh1236I	CGCG	3 cut(s)	724, 757, 803
Bsh1285I	CGRYCG	2 cut(s)	840, 924
BshFI	GGCC	5 cut(s)	503, 540, 611, 689, 797
BsiEI	CGRYCG	2 cut(s)	840, 924
BsiHKAI	GWGCWC	1 cut(s)	678
BsiSI	CCGG	6 cut(s)	120, 494, 613, 686, 714, 963
BslI	CCNNNNNNNGG	4 cut(s)	493, 554, 669, 756
BsnI	GGCC	5 cut(s)	503, 540, 611, 689, 797
Bsp1286I	GDGCHC	1 cut(s)	678
Bsp143I	GATC	6 cut(s)	16, 211, 364, 449, 635, 979
BspANI	GGCC	5 cut(s)	503, 540, 611, 689, 797
BspCNI	CTCAG	2 cut(s)	295, 841
BspFNI	CGCG	3 cut(s)	724, 757, 803
BspLI	GGNNCC	4 cut(s)	559, 690, 718, 813
BspPI	GGATC	5 cut(s)	24, 206, 359, 444, 630
BsrBI	CCGCTC	1 cut(s)	628
BsrFI	RCCGGY	1 cut(s)	713
BssAI	RCCGGY	1 cut(s)	713
BssECI	CCNNGG	7 cut(s)	34, 368, 569, 792, 801, 840, 1007
BssMI	GATC	6 cut(s)	16, 211, 364, 449, 635, 979
BssT1I	CCWWGG	2 cut(s)	368, 1007
Bst4CI	ACNGT	2 cut(s)	841, 956
Bst6I	CTCTTC	4 cut(s)	160, 197, 514, 855
BstC8I	GCNNGC	3 cut(s)	711, 715, 1004
BstDEI	CTNAG	2 cut(s)	282, 828
BstDSI	CCRYGG	2 cut(s)	801, 840
BstF5I	GGATG	2 cut(s)	373, 915
BstFNI	CGCG	3 cut(s)	724, 757, 803
BstHHI	GCGC	1 cut(s)	726
BstKTI	GATC	6 cut(s)	19, 214, 367, 452, 638, 982
BstMBI	GATC	6 cut(s)	16, 211, 364, 449, 635, 979
BstMCI	CGRYCG	2 cut(s)	840, 924
BstNSI	RCATGY	1 cut(s)	1006
BstSCI	CCNGG	2 cut(s)	611, 684
BstUI	CGCG	3 cut(s)	724, 757, 803
BstV1I	GCAGC	9 cut(s)	26, 82, 130, 152, 600, 603, 839, 861, 1012
BstV2I	GAAGAC	1 cut(s)	900
BstX2I	RGATCY	4 cut(s)	16, 211, 364, 635
BstYI	RGATCY	4 cut(s)	16, 211, 364, 635
BsuRI	GGCC	5 cut(s)	503, 540, 611, 689, 797
BtgI	CCRYGG	2 cut(s)	801, 840
BtsCI	GGATG	2 cut(s)	373, 915
BtsI	GCAGTG	1 cut(s)	1029
BtsIMutI	CAGTG	1 cut(s)	1029
Cac8I	GCNNGC	3 cut(s)	711, 715, 1004
CaiI	CAGNNNCTG	1 cut(s)	143
CfoI	GCGC	1 cut(s)	726
Cfr10I	RCCGGY	1 cut(s)	713
Cfr13I	GGNCC	5 cut(s)	31, 164, 502, 610, 688
Cfr42I	CCGCGG	1 cut(s)	804
Csp6I	GTAC	2 cut(s)	535, 834
CviAII	CATG	3 cut(s)	251, 525, 1003
CviQI	GTAC	2 cut(s)	535, 834
DdeI	CTNAG	2 cut(s)	282, 828
DpnI	GATC	6 cut(s)	18, 213, 366, 451, 637, 981
DpnII	GATC	6 cut(s)	16, 211, 364, 449, 635, 979
EaeI	YGGCCR	1 cut(s)	538
Eam1104I	CTCTTC	4 cut(s)	160, 197, 514, 855
EarI	CTCTTC	4 cut(s)	160, 197, 514, 855
EciI	GGCGGA	1 cut(s)	544
Eco130I	CCWWGG	2 cut(s)	368, 1007
Eco47I	GGWCC	2 cut(s)	31, 164
Eco57I	CTGAAG	1 cut(s)	495
EcoO109I	RGGNCCY	1 cut(s)	502
EcoT14I	CCWWGG	2 cut(s)	368, 1007
ErhI	CCWWGG	2 cut(s)	368, 1007
FaeI	CATG	3 cut(s)	254, 528, 1006
FalI	AAGNNNNNCTT	2 cut(s)	254, 286
FatI	CATG	3 cut(s)	250, 524, 1002
FauI	CCCGC	2 cut(s)	750, 784
FauNDI	CATATG	1 cut(s)	518
FokI	GGATG	2 cut(s)	360, 922
FspBI	CTAG	2 cut(s)	959, 1017
GlaI	GCGC	1 cut(s)	725
GsaI	CCCAGC	2 cut(s)	140, 403
HaeIII	GGCC	5 cut(s)	503, 540, 611, 689, 797
HapII	CCGG	6 cut(s)	120, 494, 613, 686, 714, 963
HhaI	GCGC	1 cut(s)	726
Hin1II	CATG	3 cut(s)	254, 528, 1006
Hin6I	GCGC	1 cut(s)	724
HinP1I	GCGC	1 cut(s)	724
HinfI	GANTC	3 cut(s)	764, 871, 917
HpaII	CCGG	6 cut(s)	120, 494, 613, 686, 714, 963
HphI	GGTGA	3 cut(s)	652, 689, 773
Hpy188I	TCNGA	9 cut(s)	35, 351, 514, 570, 640, 786, 831, 979, 1049
Hpy188III	TCNNGA	2 cut(s)	209, 925
Hpy99I	CGWCG	2 cut(s)	890, 991
HpyAV	CCTTC	5 cut(s)	160, 297, 592, 745, 859
HpyCH4III	ACNGT	2 cut(s)	841, 956
HpyCH4V	TGCA	4 cut(s)	250, 588, 824, 855
HpyF3I	CTNAG	2 cut(s)	282, 828
Hsp92II	CATG	3 cut(s)	254, 528, 1006
HspAI	GCGC	1 cut(s)	724
KroI	GCCGGC	1 cut(s)	713
KroNI	GCCGGC	1 cut(s)	715
KspI	CCGCGG	1 cut(s)	804
Kzo9I	GATC	6 cut(s)	16, 211, 364, 449, 635, 979
LmnI	GCTCC	6 cut(s)	47, 625, 673, 722, 811, 993
Lsp1109I	GCAGC	9 cut(s)	26, 82, 130, 152, 600, 603, 839, 861, 1012
LweI	GCATC	2 cut(s)	382, 575
MaeI	CTAG	2 cut(s)	959, 1017
MaeIII	GTNAC	3 cut(s)	658, 761, 929
MalI	GATC	6 cut(s)	18, 213, 366, 451, 637, 981
MbiI	CCGCTC	1 cut(s)	628
MboI	GATC	6 cut(s)	16, 211, 364, 449, 635, 979
MboII	GAAGA	9 cut(s)	11, 147, 184, 254, 262, 411, 501, 872, 905
MflI	RGATCY	4 cut(s)	16, 211, 364, 635
MhlI	GDGCHC	1 cut(s)	678
MluCI	AATT	1 cut(s)	245
MlyI	GAGTC	3 cut(s)	758, 880, 911
MmeI	TCCRAC	3 cut(s)	191, 663, 809
MroNI	GCCGGC	1 cut(s)	713
MroXI	GAANNNNTTC	2 cut(s)	650, 734
MseI	TTAA	2 cut(s)	222, 474
MspA1I	CMGCKG	5 cut(s)	95, 143, 803, 852, 1037
MspI	CCGG	6 cut(s)	120, 494, 613, 686, 714, 963
MspR9I	CCNGG	2 cut(s)	613, 686
MvnI	CGCG	3 cut(s)	724, 757, 803
NaeI	GCCGGC	1 cut(s)	715
NciI	CCSGG	2 cut(s)	613, 686
NdeI	CATATG	1 cut(s)	518
NdeII	GATC	6 cut(s)	16, 211, 364, 449, 635, 979
NgoMIV	GCCGGC	1 cut(s)	713
NlaIII	CATG	3 cut(s)	254, 528, 1006
NlaIV	GGNNCC	4 cut(s)	559, 690, 718, 813
NmeAIII	GCCGAG	1 cut(s)	773
NmuCI	GTSAC	3 cut(s)	658, 761, 929
NspI	RCATGY	1 cut(s)	1006
PaeI	GCATGC	1 cut(s)	1006
PcsI	WCGNNNNNNNCGW	2 cut(s)	894, 930
PdiI	GCCGGC	1 cut(s)	715
PdmI	GAANNNNTTC	2 cut(s)	650, 734
PflFI	GACNNNGTC	1 cut(s)	920
PleI	GAGTC	3 cut(s)	758, 879, 911
PpsI	GAGTC	3 cut(s)	758, 879, 911
PspFI	CCCAGC	2 cut(s)	136, 399
PspN4I	GGNNCC	4 cut(s)	559, 690, 718, 813
PspPI	GGNCC	5 cut(s)	31, 164, 502, 610, 688
PstNI	CAGNNNCTG	1 cut(s)	143
PsuI	RGATCY	4 cut(s)	16, 211, 364, 635
PsyI	GACNNNGTC	1 cut(s)	920
PvuII	CAGCTG	2 cut(s)	143, 852
RsaI	GTAC	2 cut(s)	536, 835
RsaNI	GTAC	2 cut(s)	535, 834
SacII	CCGCGG	1 cut(s)	804
SaqAI	TTAA	2 cut(s)	222, 474
Sau3AI	GATC	6 cut(s)	16, 211, 364, 449, 635, 979
Sau96I	GGNCC	5 cut(s)	31, 164, 502, 610, 688
SchI	GAGTC	3 cut(s)	758, 880, 911
ScrFI	CCNGG	2 cut(s)	613, 686
SduI	GDGCHC	1 cut(s)	678
SetI	ASST	9 cut(s)	145, 258, 474, 596, 665, 794, 829, 854, 998
SfaNI	GCATC	2 cut(s)	382, 575
Sfr303I	CCGCGG	1 cut(s)	804
SgrBI	CCGCGG	1 cut(s)	804
SinI	GGWCC	2 cut(s)	31, 164
SphI	GCATGC	1 cut(s)	1006
Sse9I	AATT	1 cut(s)	245
SspMI	CTAG	2 cut(s)	959, 1017
StyD4I	CCNGG	2 cut(s)	611, 684
StyI	CCWWGG	2 cut(s)	368, 1007
TaaI	ACNGT	2 cut(s)	841, 956
TaqI	TCGA	2 cut(s)	596, 986
TaqII	GACCGA	1 cut(s)	910
TasI	AATT	1 cut(s)	245
TauI	GCSGC	1 cut(s)	1037
Tru1I	TTAA	2 cut(s)	222, 474
Tru9I	TTAA	2 cut(s)	222, 474
TscAI	CASTG	1 cut(s)	1036
TseFI	GTSAC	3 cut(s)	658, 761, 929
TseI	GCWGC	9 cut(s)	39, 95, 140, 143, 588, 591, 849, 852, 1025
Tsp45I	GTSAC	3 cut(s)	658, 761, 929
TspDTI	ATGAA	2 cut(s)	147, 643
TspRI	CASTG	1 cut(s)	1036
Tth111I	GACNNNGTC	1 cut(s)	920
VpaK11BI	GGWCC	2 cut(s)	31, 164
XceI	RCATGY	1 cut(s)	1006
XmnI	GAANNNNTTC	2 cut(s)	650, 734
XspI	CTAG	2 cut(s)	959, 1017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.