Rroxscaffold_4G00298610
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013949
Physical Location & Seq
Reverse (-)
18392812 .. 18394667
1856 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00298610.1

Sequence Viewer

Length: 993 bp
ATGGAAGACGATTGGGATCTCACAGCCGTGGTCAGAGGCTGCTCCACCGTCCCAACCACCACCACCGCCAACAAGCCAACCAACCCTTTCATCACAGACCACCTCCACACAAGCCCTCTCCTATCTTCATTTGGTCAACAGGGTTTTGTCTCCAACAACAACACTCAAACCTTCTCTGTTCTTCCTCTCCCTCCAGATCCCATTAAACCCACAAGTGCAATTGAGGAACTGCATGATCTTTACAAGCCATTCTTCCCCAAATCTCACCAAACCCCTTCTCTTCTTTCTCCACCCCCACAAATCACACCACAGCCCAGTTTCTCTTCCCCTCAACCTCTCCACAGACACCAACAACATCAGCCTAAGCTTTCTGCTACTAATCCCCAGCGCTCCAAAAAGAGAAAGAACCAGCTTAAGAAGGTGTGCCAAGTACCAGCGGAGTCTTTGTCTTCTGATGTGTGGGCATGGCGTAAATACGGACAGAAACCCATCAAGGGCTCACCATATCCGAGAGGTTACTACAGATGCAGCAGCTCAAAGGGTTGTATGGCCCGGAAGCAAGTGGAGCGGAACAGATCCGACCCGGGAATGTTCATAGTCACCTACACGGCGGAGCACAACCACCCTGCACCGACTCACCGCAACTCGCTTGCTGGGTCCACGCGTCACAAGCCTGTCTCTTCTCCCCAGTCGGGTTCCGTCACGGGCGGTGACTCTACCAAACCCAAGGGCTGCTCTCCGTCGCCGGCCACGTCGGCGGAGGAGGAGCTTGGTCGGAGCACGACAAGGATGGAGGCGAAAGAAGAAAAACAGAGTCCTATGGCGGATGATGACGAAACAGAGTACTTTGGGGCGTGTGATACGATTGTGAGCGATGATTTCTTTGTGGGTTTGGAAGGTCTCGCCGGAGATTACTTTTCCGATGACATTACGGCCAGCTTCGGCGGCGCATGGAGTTCTGGTAATGCTGCCACCGCCGCCAGTAGTATCTGA

Protein Analysis

330

Amino Acids

35.95

Weight (kDa)

7.68

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 152 - 209 1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01250
fragaria_vesca FvH4_7g15350
malus_domestica MD01G1071300.v1.1 MD07G1131400.v1.1
prunus_persica Prupe.2G177400_v2.0.a1
pyrus_communis pycom01g10030
rosa_chinensis RchiOBHm_Chr1g0357671
rosa_laevigata RLG00000028008
rosa_multiflora Rmu_co8407323.1_g000001 Rmu_ssc0000425.1_g000001
rosa_roxburghii Rroxscaffold_4G00298610
rosa_rugosa Rorug01G0257600
rosa_samantha Rh1AG271600 Rh1BG238700 Rh1CG255500 Rh1DG267100
rosa_wichuraiana Rw1G024220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 568
AccII CGCG 1 cut(s) 664
AclWI GGATC 3 cut(s) 24, 191, 570
AcoI YGGCCR 2 cut(s) 747, 933
AfaI GTAC 2 cut(s) 432, 845
AfeI AGCGCT 1 cut(s) 389
AfiI CCNNNNNNNGG 2 cut(s) 494, 692
AflII CTTAAG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 662
AjiI CACGTC 1 cut(s) 753
AleI CACNNNNGTG 1 cut(s) 26
AluBI AGCT 5 cut(s) 367, 412, 534, 769, 939
AluI AGCT 5 cut(s) 367, 412, 534, 769, 939
Alw21I GWGCWC 2 cut(s) 618, 782
Alw26I GTCTC 3 cut(s) 154, 682, 905
AlwI GGATC 3 cut(s) 24, 191, 570
AlwNI CAGNNNCTG 1 cut(s) 39
Ama87I CYCGRG 1 cut(s) 583
Aor51HI AGCGCT 1 cut(s) 389
AoxI GGCC 3 cut(s) 549, 747, 933
ApeKI GCWGC 5 cut(s) 39, 528, 531, 732, 968
AspLEI GCGC 2 cut(s) 390, 950
AspS9I GGNCC 2 cut(s) 550, 657
AsuC2I CCSGG 3 cut(s) 553, 584, 585
AsuHPI GGTGA 5 cut(s) 257, 492, 592, 629, 722
AvaI CYCGRG 1 cut(s) 583
AvaII GGWCC 1 cut(s) 657
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BanII GRGCYC 1 cut(s) 500
BbsI GAAGAC 2 cut(s) 12, 441
Bbv12I GWGCWC 2 cut(s) 618, 782
BbvI GCAGC 5 cut(s) 26, 540, 543, 719, 955
BccI CCATC 2 cut(s) 497, 784
BceAI ACGGC 3 cut(s) 11, 624, 948
BcnI CCSGG 3 cut(s) 553, 584, 585
BcoDI GTCTC 3 cut(s) 154, 682, 905
BfmI CTRYAG 1 cut(s) 520
BfoI RGCGCY 1 cut(s) 391
BfrI CTTAAG 1 cut(s) 413
BglI GCCNNNNNGGC 1 cut(s) 755
BisI GCNGC 7 cut(s) 40, 529, 532, 733, 946, 969, 978
BlsI GCNGC 7 cut(s) 41, 530, 533, 734, 947, 970, 979
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 3 cut(s) 553, 584, 585
Bme18I GGWCC 1 cut(s) 657
BmeT110I CYCGRG 1 cut(s) 583
BmgBI CACGTC 1 cut(s) 753
BmgT120I GGNCC 2 cut(s) 550, 657
BmiI GGNNCC 2 cut(s) 658, 697
BmrFI CCNGG 3 cut(s) 553, 584, 585
BmrI ACTGGG 2 cut(s) 309, 682
BmsI GCATC 1 cut(s) 515
BmuI ACTGGG 2 cut(s) 309, 682
BpiI GAAGAC 2 cut(s) 12, 441
BpmI CTGGAG 1 cut(s) 177
Bpu10I CCTNAGC 1 cut(s) 363
BpuMI CCSGG 3 cut(s) 553, 584, 585
BsaBI GATNNNNATC 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 905
BsaJI CCNNGG 3 cut(s) 27, 583, 726
BsaXI ACNNNNNCTCC 4 cut(s) 431, 461, 946, 976
Bsc4I CCNNNNNNNGG 2 cut(s) 494, 692
Bse118I RCCGGY 1 cut(s) 745
Bse1I ACTGG 3 cut(s) 315, 688, 981
Bse8I GATNNNNATC 1 cut(s) 15
BseDI CCNNGG 3 cut(s) 27, 583, 726
BseGI GGATG 2 cut(s) 795, 832
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 2 cut(s) 494, 692
BseNI ACTGG 3 cut(s) 315, 688, 981
BseRI GAGGAG 2 cut(s) 776, 779
BseXI GCAGC 5 cut(s) 26, 540, 543, 719, 955
BseYI CCCAGC 2 cut(s) 384, 653
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 664
BshFI GGCC 3 cut(s) 551, 749, 935
BsiHKAI GWGCWC 2 cut(s) 618, 782
BsiHKCI CYCGRG 1 cut(s) 583
BsiSI CCGG 4 cut(s) 553, 584, 746, 906
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 494, 692
BsmAI GTCTC 3 cut(s) 154, 682, 905
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 3 cut(s) 551, 749, 935
Bso31I GGTCTC 1 cut(s) 905
BsoBI CYCGRG 1 cut(s) 583
Bsp1286I GDGCHC 3 cut(s) 500, 618, 782
Bsp143I GATC 4 cut(s) 16, 196, 235, 575
BspANI GGCC 3 cut(s) 551, 749, 935
BspFNI CGCG 1 cut(s) 664
BspLI GGNNCC 2 cut(s) 658, 697
BspPI GGATC 3 cut(s) 24, 191, 570
BspTI CTTAAG 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 905
BsrBI CCGCTC 1 cut(s) 568
BsrFI RCCGGY 1 cut(s) 745
BsrI ACTGG 3 cut(s) 315, 688, 981
BssAI RCCGGY 1 cut(s) 745
BssECI CCNNGG 3 cut(s) 27, 583, 726
BssMI GATC 4 cut(s) 16, 196, 235, 575
BssT1I CCWWGG 1 cut(s) 726
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 3 cut(s) 285, 328, 685
BstAFI CTTAAG 1 cut(s) 413
BstC8I GCNNGC 3 cut(s) 651, 747, 937
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 795, 832
BstFNI CGCG 1 cut(s) 664
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 2 cut(s) 390, 950
BstKTI GATC 4 cut(s) 19, 199, 238, 578
BstMAI GTCTC 3 cut(s) 154, 682, 905
BstMBI GATC 4 cut(s) 16, 196, 235, 575
BstMWI GCNNNNNNNGC 6 cut(s) 565, 670, 755, 945, 974, 977
BstSCI CCNGG 3 cut(s) 551, 582, 583
BstSFI CTRYAG 1 cut(s) 520
BstUI CGCG 1 cut(s) 664
BstV1I GCAGC 5 cut(s) 26, 540, 543, 719, 955
BstV2I GAAGAC 2 cut(s) 12, 441
BstX2I RGATCY 3 cut(s) 16, 196, 575
BstYI RGATCY 3 cut(s) 16, 196, 575
BsuRI GGCC 3 cut(s) 551, 749, 935
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 888
BtrI CACGTC 1 cut(s) 753
BtsCI GGATG 2 cut(s) 795, 832
Cac8I GCNNGC 3 cut(s) 651, 747, 937
CaiI CAGNNNCTG 1 cut(s) 39
CfoI GCGC 2 cut(s) 390, 950
Cfr10I RCCGGY 1 cut(s) 745
Cfr13I GGNCC 2 cut(s) 550, 657
Cfr9I CCCGGG 1 cut(s) 583
CseI GACGC 1 cut(s) 653
Csp6I GTAC 2 cut(s) 431, 844
CviAII CATG 3 cut(s) 233, 465, 951
CviQI GTAC 2 cut(s) 431, 844
DdeI CTNAG 1 cut(s) 363
DpnI GATC 4 cut(s) 18, 198, 237, 577
DpnII GATC 4 cut(s) 16, 196, 235, 575
EaeI YGGCCR 2 cut(s) 747, 933
Eam1104I CTCTTC 3 cut(s) 285, 328, 685
EarI CTCTTC 3 cut(s) 285, 328, 685
EciI GGCGGA 3 cut(s) 626, 773, 839
Eco130I CCWWGG 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 500
Eco31I GGTCTC 1 cut(s) 905
Eco47I GGWCC 1 cut(s) 657
Eco47III AGCGCT 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 583
EcoT14I CCWWGG 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 500
ErhI CCWWGG 1 cut(s) 726
FaeI CATG 3 cut(s) 236, 468, 954
FaiI YATR 7 cut(s) 234, 466, 505, 548, 596, 821, 952
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 35
FatI CATG 3 cut(s) 232, 464, 950
Fnu4HI GCNGC 7 cut(s) 40, 529, 532, 733, 946, 969, 978
FokI GGATG 2 cut(s) 802, 839
FriOI GRGCYC 1 cut(s) 500
Fsp4HI GCNGC 7 cut(s) 40, 529, 532, 733, 946, 969, 978
GlaI GCGC 2 cut(s) 389, 949
GluI GCNGC 7 cut(s) 40, 529, 532, 733, 946, 969, 978
GsaI CCCAGC 2 cut(s) 388, 657
GsuI CTGGAG 1 cut(s) 177
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 3 cut(s) 551, 749, 935
HapII CCGG 4 cut(s) 553, 584, 746, 906
HgaI GACGC 1 cut(s) 653
HhaI GCGC 2 cut(s) 390, 950
Hin1II CATG 3 cut(s) 236, 468, 954
Hin6I GCGC 2 cut(s) 388, 948
HinP1I GCGC 2 cut(s) 388, 948
HincII GTYRAC 1 cut(s) 137
HindII GTYRAC 1 cut(s) 137
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 4 cut(s) 440, 634, 713, 814
HpaII CCGG 4 cut(s) 553, 584, 746, 906
HphI GGTGA 5 cut(s) 257, 492, 592, 629, 722
Hpy166II GTNNAC 2 cut(s) 137, 660
Hpy188I TCNGA 7 cut(s) 35, 454, 510, 580, 777, 922, 992
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 2 cut(s) 137, 660
Hpy99I CGWCG 2 cut(s) 745, 757
HpyAV CCTTC 4 cut(s) 181, 285, 412, 890
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 752
HpyCH4V TGCA 4 cut(s) 218, 232, 528, 629
HpyF10VI GCNNNNNNNGC 6 cut(s) 565, 670, 755, 945, 974, 977
HpyF3I CTNAG 1 cut(s) 363
HpySE526I ACGT 1 cut(s) 752
Hsp92II CATG 3 cut(s) 236, 468, 954
HspAI GCGC 2 cut(s) 388, 948
KroI GCCGGC 1 cut(s) 745
KroNI GCCGGC 1 cut(s) 747
Kzo9I GATC 4 cut(s) 16, 196, 235, 575
LmnI GCTCC 6 cut(s) 47, 395, 565, 613, 766, 777
Lsp1109I GCAGC 5 cut(s) 26, 540, 543, 719, 955
LweI GCATC 1 cut(s) 515
MaeII ACGT 1 cut(s) 752
MaeIII GTNAC 5 cut(s) 515, 598, 665, 700, 710
MalI GATC 4 cut(s) 18, 198, 237, 577
MbiI CCGCTC 1 cut(s) 568
MboI GATC 4 cut(s) 16, 196, 235, 575
MboII GAAGA 9 cut(s) 17, 117, 173, 244, 272, 315, 441, 672, 815
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 3 cut(s) 16, 196, 575
MhlI GDGCHC 3 cut(s) 500, 618, 782
MluCI AATT 1 cut(s) 219
MluI ACGCGT 1 cut(s) 662
MlyI GAGTC 4 cut(s) 449, 628, 707, 823
MmeI TCCRAC 3 cut(s) 177, 603, 755
MroNI GCCGGC 1 cut(s) 745
MseI TTAA 2 cut(s) 204, 414
MslI CAYNNNNRTG 1 cut(s) 26
MspA1I CMGCKG 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 4 cut(s) 553, 584, 746, 906
MspR9I CCNGG 3 cut(s) 553, 584, 585
MunI CAATTG 1 cut(s) 219
MvnI CGCG 1 cut(s) 664
MwoI GCNNNNNNNGC 6 cut(s) 565, 670, 755, 945, 974, 977
NaeI GCCGGC 1 cut(s) 747
NciI CCSGG 3 cut(s) 553, 584, 585
NdeII GATC 4 cut(s) 16, 196, 235, 575
NgoMIV GCCGGC 1 cut(s) 745
NlaIII CATG 3 cut(s) 236, 468, 954
NlaIV GGNNCC 2 cut(s) 658, 697
NmuCI GTSAC 4 cut(s) 598, 665, 700, 710
OliI CACNNNNGTG 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 749
PdiI GCCGGC 1 cut(s) 747
PkrI GCNGC 7 cut(s) 41, 530, 533, 734, 947, 970, 979
PleI GAGTC 4 cut(s) 448, 628, 707, 822
PpsI GAGTC 4 cut(s) 448, 628, 707, 822
PspFI CCCAGC 2 cut(s) 384, 653
PspN4I GGNNCC 2 cut(s) 658, 697
PspPI GGNCC 2 cut(s) 550, 657
PstNI CAGNNNCTG 1 cut(s) 39
PsuI RGATCY 3 cut(s) 16, 196, 575
RsaI GTAC 2 cut(s) 432, 845
RsaNI GTAC 2 cut(s) 431, 844
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 2 cut(s) 204, 414
SatI GCNGC 7 cut(s) 40, 529, 532, 733, 946, 969, 978
Sau3AI GATC 4 cut(s) 16, 196, 235, 575
Sau96I GGNCC 2 cut(s) 550, 657
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 4 cut(s) 449, 628, 707, 823
ScrFI CCNGG 3 cut(s) 553, 584, 585
SduI GDGCHC 3 cut(s) 500, 618, 782
SfaNI GCATC 1 cut(s) 515
SfcI CTRYAG 1 cut(s) 520
SinI GGWCC 1 cut(s) 657
SmaI CCCGGG 1 cut(s) 585
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 1 cut(s) 219
StyD4I CCNGG 3 cut(s) 551, 582, 583
StyI CCWWGG 1 cut(s) 726
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 1 cut(s) 755
TasI AATT 1 cut(s) 219
TatI WGTACW 1 cut(s) 843
TauI GCSGC 2 cut(s) 948, 980
Tru1I TTAA 2 cut(s) 204, 414
Tru9I TTAA 2 cut(s) 204, 414
TseFI GTSAC 4 cut(s) 598, 665, 700, 710
TseI GCWGC 5 cut(s) 39, 528, 531, 732, 968
Tsp45I GTSAC 4 cut(s) 598, 665, 700, 710
TspDTI ATGAA 3 cut(s) 79, 117, 583
TspGWI ACGGA 3 cut(s) 492, 688, 729
TspMI CCCGGG 1 cut(s) 583
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 657
XmaI CCCGGG 1 cut(s) 583
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.