AT4G01600

GEM-like protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008764
Physical Location & Seq
Forward (+)
692853 .. 694381
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G01600.3

Sequence Viewer

Length: 702 bp
ATGCGTCCACAATACAAAGACGTGAACGATAAATTAGACTCGACGGCCAAGCACAAAACCAACAGCCATGGCGGTCGTTATGATAATCCTTACGTCCACATTACTAGTCCCACATCAGCTTCTGATAAGAGGTCGAAGGATAAGGTTCTTGAGGTGTTGAATCGCTGTGGGAAGAAAGTAGAGGACGCTACTCGCAAAGCGGAAGCATTAGTCGGAGGTTTAAAAGACCACCTGAAGTTTAGTCCTAGCATAAGTGATGCAGCAATGGCTAGGCTTTCTCAAGGCACTAAGATGATCGTTGAAGGAGGACCAGAGAGAGTGTTTCAACGAGAGTTTGGTGTTTTAGCTGTAGAGAAACTTTTAGATTCGTTTGTTTGCTACATATCGACTACTTCGGGACCAGTGACCGGAGTGATATACATTTCCAACAGAAGAATCGCTTTTTGTAGCGACTATGCCATTCGTCTTCCTTCTTCTGCCGGTGGAAACGGTGTTGCGGCATATTACAAGGTGGTGATGGAGTGGGAGAAGATAAGTAGCATTAGTTCATCAACGAACGTGTTGAAGCCAAGTGAGAGATACGTGCATATGGTGACACGAGATGGGTTTGAGTTTTGGTTCATGGGCTTTGTCTCCTACATCGATGCTTTCAATTGTCTCAACAAAGCTCTCCTCAATTCCCGTTGTAATCATGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

233

Amino Acids

25.91

Weight (kDa)

9.21

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 68 - 185 1.9e-13 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 72, 200, 497
AcoI YGGCCR 1 cut(s) 45
AcuI CTGAAG 1 cut(s) 254
AfiI CCNNNNNNNGG 1 cut(s) 407
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 5 cut(s) 160, 302, 326, 565, 652
AhlI ACTAGT 1 cut(s) 104
AjiI CACGTC 1 cut(s) 22
AluBI AGCT 3 cut(s) 119, 347, 668
AluI AGCT 3 cut(s) 119, 347, 668
Alw26I GTCTC 2 cut(s) 637, 662
AlwNI CAGNNNCTG 1 cut(s) 122
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 260
AspS9I GGNCC 2 cut(s) 308, 398
AsuHPI GGTGA 2 cut(s) 526, 604
AvaII GGWCC 2 cut(s) 308, 398
BauI CACGAG 1 cut(s) 597
BbsI GAAGAC 1 cut(s) 458
BbvI GCAGC 1 cut(s) 272
BccI CCATC 2 cut(s) 511, 596
BceAI ACGGC 1 cut(s) 60
BcoDI GTCTC 2 cut(s) 637, 662
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 3 cut(s) 105, 246, 270
BfmI CTRYAG 1 cut(s) 348
BisI GCNGC 2 cut(s) 261, 498
BlsI GCNGC 2 cut(s) 262, 499
Bme18I GGWCC 2 cut(s) 308, 398
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 308, 398
BmiI GGNNCC 1 cut(s) 399
BmsI GCATC 2 cut(s) 247, 634
BpiI GAAGAC 1 cut(s) 458
BpuEI CTTGAG 2 cut(s) 170, 264
Bsa29I ATCGAT 1 cut(s) 642
BsaAI YACGTR 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 407
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse118I RCCGGY 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 270
BseCI ATCGAT 1 cut(s) 642
BseDI CCNNGG 1 cut(s) 67
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 401
BseRI GAGGAG 1 cut(s) 662
BseXI GCAGC 1 cut(s) 272
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 1 cut(s) 47
BshVI ATCGAT 1 cut(s) 642
BsiEI CGRYCG 1 cut(s) 76
BsiSI CCGG 2 cut(s) 408, 480
BslFI GGGAC 2 cut(s) 93, 411
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 2 cut(s) 637, 662
BsmFI GGGAC 2 cut(s) 93, 411
BsnI GGCC 1 cut(s) 47
Bsp143I GATC 1 cut(s) 294
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 3 cut(s) 72, 200, 497
BspANI GGCC 1 cut(s) 47
BspDI ATCGAT 1 cut(s) 642
BspLI GGNNCC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 479
BsrI ACTGG 1 cut(s) 401
BssAI RCCGGY 1 cut(s) 479
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 1 cut(s) 294
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 67
Bst2BI CACGAG 1 cut(s) 597
Bst4CI ACNGT 1 cut(s) 491
BstBAI YACGTR 1 cut(s) 583
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 67
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 2 cut(s) 637, 662
BstMBI GATC 1 cut(s) 294
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 272
BstV2I GAAGAC 1 cut(s) 458
Bsu15I ATCGAT 1 cut(s) 642
BsuRI GGCC 1 cut(s) 47
BsuTUI ATCGAT 1 cut(s) 642
BtgI CCRYGG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 22
BtsIMutI CAGTG 1 cut(s) 408
CaiI CAGNNNCTG 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 308, 398
ClaI ATCGAT 1 cut(s) 642
CseI GACGC 1 cut(s) 194
CviAII CATG 4 cut(s) 68, 622, 692, 697
CviJI RGCY 9 cut(s) 47, 66, 119, 269, 274, 347, 568, 627, 668
CviKI_1 RGCY 9 cut(s) 47, 66, 119, 269, 274, 347, 568, 627, 668
DdeI CTNAG 1 cut(s) 288
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
DraI TTTAAA 1 cut(s) 222
EaeI YGGCCR 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 67
Eco47I GGWCC 2 cut(s) 308, 398
Eco57I CTGAAG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 4 cut(s) 71, 625, 695, 700
FalI AAGNNNNNCTT 2 cut(s) 424, 456
FaqI GGGAC 2 cut(s) 93, 411
FatI CATG 4 cut(s) 67, 621, 691, 696
FauNDI CATATG 1 cut(s) 588
Fnu4HI GCNGC 2 cut(s) 261, 498
Fsp4HI GCNGC 2 cut(s) 261, 498
FspBI CTAG 3 cut(s) 105, 246, 270
GluI GCNGC 2 cut(s) 261, 498
HaeIII GGCC 1 cut(s) 47
HapII CCGG 2 cut(s) 408, 480
HgaI GACGC 1 cut(s) 194
Hin1II CATG 4 cut(s) 71, 625, 695, 700
HinfI GANTC 4 cut(s) 38, 160, 365, 435
HpaII CCGG 2 cut(s) 408, 480
HphI GGTGA 2 cut(s) 526, 604
Hpy166II GTNNAC 3 cut(s) 8, 25, 97
Hpy188I TCNGA 2 cut(s) 124, 215
Hpy188III TCNNGA 2 cut(s) 149, 396
Hpy8I GTNNAC 3 cut(s) 8, 25, 97
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 130, 296, 480
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4IV ACGT 4 cut(s) 21, 93, 558, 582
HpyCH4V TGCA 2 cut(s) 260, 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 4 cut(s) 21, 93, 558, 582
Hsp92II CATG 4 cut(s) 71, 625, 695, 700
Kzo9I GATC 1 cut(s) 294
LpnPI CCDG 5 cut(s) 245, 324, 414, 421, 493
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 2 cut(s) 247, 634
MaeI CTAG 3 cut(s) 105, 246, 270
MaeII ACGT 4 cut(s) 21, 93, 558, 582
MaeIII GTNAC 2 cut(s) 403, 592
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 5 cut(s) 184, 444, 458, 465, 541
MfeI CAATTG 1 cut(s) 652
MluCI AATT 3 cut(s) 32, 652, 676
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 2 cut(s) 193, 450
MnlI CCTC 6 cut(s) 123, 145, 175, 209, 299, 683
MseI TTAA 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 290
MspI CCGG 2 cut(s) 408, 480
MunI CAATTG 1 cut(s) 652
MwoI GCNNNNNNNGC 1 cut(s) 266
NcoI CCATGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 588
NdeII GATC 1 cut(s) 294
NlaIII CATG 4 cut(s) 71, 625, 695, 700
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 2 cut(s) 403, 592
PfeI GAWTC 3 cut(s) 160, 365, 435
PkrI GCNGC 2 cut(s) 262, 499
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Ppu21I YACGTR 1 cut(s) 583
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 2 cut(s) 308, 398
PsrI GAACNNNNNNTAC 2 cut(s) 529, 561
PstNI CAGNNNCTG 1 cut(s) 122
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 2 cut(s) 261, 498
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 2 cut(s) 308, 398
SchI GAGTC 1 cut(s) 32
SfaNI GCATC 2 cut(s) 247, 634
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 2 cut(s) 308, 398
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 2 cut(s) 149, 279
SmoI CTYRAG 2 cut(s) 149, 279
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 3 cut(s) 32, 652, 676
SsiI CCGC 3 cut(s) 72, 200, 497
SspMI CTAG 3 cut(s) 105, 246, 270
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 491
TaiI ACGT 4 cut(s) 24, 96, 561, 585
TaqI TCGA 4 cut(s) 41, 134, 386, 642
TasI AATT 3 cut(s) 32, 652, 676
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 3 cut(s) 160, 365, 435
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TscAI CASTG 1 cut(s) 408
TseFI GTSAC 2 cut(s) 403, 592
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 2 cut(s) 403, 592
TspDTI ATGAA 2 cut(s) 537, 610
TspRI CASTG 1 cut(s) 408
VpaK11BI GGWCC 2 cut(s) 308, 398
XspI CTAG 3 cut(s) 105, 246, 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.