pycom03g06000

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008764
Physical Location & Seq
Reverse (-)
4750771 .. 4751391
621 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06000.1

Sequence Viewer

Length: 417 bp
ATGGCTAATATGGTTGAGGCATTGTGCCAGTGCGGTAAAAGGGTTGAAGTTGCCACCAGAAGAGCAAAAGGCCAAGGCGGACAATGTCTGGAACCACCAACGTGCCCGAGTTTCACAGAAGCAATGATGGCAAGGCTAAATACCGGAACAAAGGTGCTTACTGCCGGAGGAGAAGAAAAAGTGTTTCAGCAGACATTTGAAAGACTGACAGGAGAAAAGCTCTTACATTCATATGCTTGCTATCTATCAACTTCTCATAGACCTGTGATTGGGATGCTGTACAGCTCCGATAAACGAATAGTCTTCTGCAGCGATTTCTCTTACTGTTTGTGCCCAGGAAATCCAACTCTCATGCATTACAAAGTAGATATTCTAGTGGACATAAAATTCGAATTCTTAAGTTTTTCATTCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

139

Amino Acids

15.42

Weight (kDa)

8.12

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 61 - 109 4.2e-08 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 33, 78
AcsI RAATTY 2 cut(s) 386, 392
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 269
AflII CTTAAG 1 cut(s) 397
AgsI TTSAA 2 cut(s) 47, 200
AjnI CCWGG 1 cut(s) 334
AleI CACNNNNGTG 1 cut(s) 100
AluBI AGCT 2 cut(s) 220, 285
AluI AGCT 2 cut(s) 220, 285
Ama87I CYCGRG 1 cut(s) 106
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 1 cut(s) 309
ApoI RAATTY 2 cut(s) 386, 392
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AsuII TTCGAA 1 cut(s) 390
AvaI CYCGRG 1 cut(s) 106
BaeGI GKGCMC 2 cut(s) 107, 335
BbsI GAAGAC 1 cut(s) 295
BbvI GCAGC 1 cut(s) 321
BccI CCATC 1 cut(s) 121
BciT130I CCWGG 1 cut(s) 336
BfaI CTAG 1 cut(s) 374
BfmI CTRYAG 1 cut(s) 307
BfrI CTTAAG 1 cut(s) 397
BisI GCNGC 1 cut(s) 310
BlsI GCNGC 1 cut(s) 311
Bme1390I CCNGG 1 cut(s) 336
BmeT110I CYCGRG 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 336
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 295
BplI GAGNNNNNCTC 2 cut(s) 204, 236
Bpu14I TTCGAA 1 cut(s) 390
BsaJI CCNNGG 2 cut(s) 73, 334
BsaWI WCCGGW 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 1 cut(s) 336
BseDI CCNNGG 2 cut(s) 73, 334
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 2 cut(s) 107, 335
BseXI GCAGC 1 cut(s) 321
BshFI GGCC 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 2 cut(s) 144, 165
BslI CCNNNNNNNGG 1 cut(s) 269
BsnI GGCC 1 cut(s) 72
BsoBI CYCGRG 1 cut(s) 106
Bsp119I TTCGAA 1 cut(s) 390
Bsp1286I GDGCHC 2 cut(s) 107, 335
Bsp1407I TGTACA 1 cut(s) 279
BspACI CCGC 2 cut(s) 33, 78
BspANI GGCC 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 93
BspMAI CTGCAG 1 cut(s) 311
BspQI GCTCTTC 1 cut(s) 55
BspT104I TTCGAA 1 cut(s) 390
BspTI CTTAAG 1 cut(s) 397
BsrDI GCAATG 1 cut(s) 129
BsrGI TGTACA 1 cut(s) 279
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 2 cut(s) 73, 334
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 1 cut(s) 55
BstAFI CTTAAG 1 cut(s) 397
BstAUI TGTACA 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 390
BstC8I GCNNGC 1 cut(s) 238
BstF5I GGATG 1 cut(s) 279
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 334
BstSFI CTRYAG 1 cut(s) 307
BstSLI GKGCMC 2 cut(s) 107, 335
BstV1I GCAGC 1 cut(s) 321
BstV2I GAAGAC 1 cut(s) 295
BsuRI GGCC 1 cut(s) 72
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 280
CviAII CATG 1 cut(s) 352
CviJI RGCY 5 cut(s) 5, 72, 136, 220, 285
CviKI_1 RGCY 5 cut(s) 5, 72, 136, 220, 285
CviQI GTAC 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 106
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 73
EcoT22I ATGCAT 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 1 cut(s) 355
FaiI YATR 6 cut(s) 11, 232, 234, 258, 353, 383
FatI CATG 1 cut(s) 351
FauNDI CATATG 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 310
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 310
FspBI CTAG 1 cut(s) 374
GluI GCNGC 1 cut(s) 310
HaeIII GGCC 1 cut(s) 72
HapII CCGG 2 cut(s) 144, 165
Hin1II CATG 1 cut(s) 355
HpaII CCGG 2 cut(s) 144, 165
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 1 cut(s) 89
Hpy8I GTNNAC 1 cut(s) 379
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 2 cut(s) 309, 355
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 1 cut(s) 355
LguI GCTCTTC 1 cut(s) 55
LmnI GCTCC 1 cut(s) 290
LpnPI CCDG 9 cut(s) 41, 70, 74, 157, 178, 195, 276, 321, 348
Lsp1109I GCAGC 1 cut(s) 321
LweI GCATC 1 cut(s) 264
MaeI CTAG 1 cut(s) 374
MaeII ACGT 1 cut(s) 101
MboII GAAGA 3 cut(s) 72, 185, 295
MhlI GDGCHC 2 cut(s) 107, 335
MluCI AATT 2 cut(s) 386, 392
MmeI TCCRAC 1 cut(s) 368
MnlI CCTC 2 cut(s) 10, 161
Mph1103I ATGCAT 1 cut(s) 357
MseI TTAA 1 cut(s) 398
MslI CAYNNNNRTG 2 cut(s) 100, 231
MspCI CTTAAG 1 cut(s) 397
MspI CCGG 2 cut(s) 144, 165
MspR9I CCNGG 1 cut(s) 336
MvaI CCWGG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 128
NdeI CATATG 1 cut(s) 232
NlaIII CATG 1 cut(s) 355
NlaIV GGNNCC 1 cut(s) 93
NsiI ATGCAT 1 cut(s) 357
NspV TTCGAA 1 cut(s) 390
OliI CACNNNNGTG 1 cut(s) 100
PciSI GCTCTTC 1 cut(s) 55
PflFI GACNNNGTC 1 cut(s) 84
PkrI GCNGC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 334
PspGI CCWGG 1 cut(s) 334
PspN4I GGNNCC 1 cut(s) 93
PstI CTGCAG 1 cut(s) 311
PsyI GACNNNGTC 1 cut(s) 84
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 2 cut(s) 100, 231
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 1 cut(s) 310
ScrFI CCNGG 1 cut(s) 336
SduI GDGCHC 2 cut(s) 107, 335
SetI ASST 5 cut(s) 104, 156, 222, 265, 287
SfaNI GCATC 1 cut(s) 264
SfcI CTRYAG 1 cut(s) 307
SfuI TTCGAA 1 cut(s) 390
SmiMI CAYNNNNRTG 2 cut(s) 100, 231
SmlI CTYRAG 1 cut(s) 397
SmoI CTYRAG 1 cut(s) 397
Sse9I AATT 2 cut(s) 386, 392
SsiI CCGC 2 cut(s) 33, 78
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 1 cut(s) 334
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 104
TaqI TCGA 1 cut(s) 390
TasI AATT 2 cut(s) 386, 392
TatI WGTACW 1 cut(s) 279
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TscAI CASTG 1 cut(s) 35
TseI GCWGC 1 cut(s) 309
TspDTI ATGAA 2 cut(s) 219, 396
TspRI CASTG 1 cut(s) 35
Tth111I GACNNNGTC 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 397
XapI RAATTY 2 cut(s) 386, 392
XspI CTAG 1 cut(s) 374
Zsp2I ATGCAT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.