MD03G1075400.v1.1

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008764
Physical Location & Seq
Reverse (-)
6134675 .. 6135472
798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1075400.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGCTAATATGGTTGAGGCATTGTGCCAGTGCGGTAAAAGGGTTGAAGTTGCCACCAGAAGAGCAGAGGCCAAGGCGGAAAATGTCTGGAACCACCACGCAATGATGGCAAGGCTAAATACCGGAACAAAGGTGCTTACTGCTGGAGGAGAAGAAAAAGTGTTTCAGCAGACATTTGAAAGAATGACAGGAGAAAAGCTCTTACATTCATATGCTTGCTATCTATCAACTTCTCATGGACCTGTGATTGGGATGTTGTACATCTCCGATAAACGAATAGCCTTCTGCAGCGATTTCTCTTACTATTTGTGCCCAGAAAATCCAACTCTCATGCATTACAAGTTAGGTATTCTAGTGGTGGTGCCGCTTGATCAGTTAAGAACAGTAAATCCTTCAGCAAACAGTTGGAACCCTTCAGAAAAATATATACAGATTGTCACAAGGGATGGTCATGAATTCTGGTTCATGGGGTTCATATCCTATGACAAAGCCCTCAGGAATCTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

170

Amino Acids

19.28

Weight (kDa)

8.26

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 54 - 164 2.1e-15 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 361
AciI CCGC 3 cut(s) 33, 77, 365
AcsI RAATTY 1 cut(s) 455
AcuI CTGAAG 2 cut(s) 378, 399
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 248
AgsI TTSAA 2 cut(s) 47, 179
AloI GAACNNNNNNTCC 2 cut(s) 373, 405
AluBI AGCT 1 cut(s) 199
AluI AGCT 1 cut(s) 199
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 1 cut(s) 288
ApoI RAATTY 1 cut(s) 455
AspS9I GGNCC 1 cut(s) 239
AvaII GGWCC 1 cut(s) 239
AxyI CCTNAGG 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 314
BanI GGYRCC 1 cut(s) 361
BbvI GCAGC 1 cut(s) 300
BccI CCATC 2 cut(s) 100, 440
BclI TGATCA 1 cut(s) 370
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 286
BisI GCNGC 2 cut(s) 289, 365
BlsI GCNGC 2 cut(s) 290, 366
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 3 cut(s) 92, 363, 410
BplI GAGNNNNNCTC 2 cut(s) 183, 215
BpmI CTGGAG 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 122
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 28
Bse21I CCTNAGG 1 cut(s) 494
Bse3DI GCAATG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 2 cut(s) 258, 451
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 508
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 162
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 1 cut(s) 300
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 361
BsiSI CCGG 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 248
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 314
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 1 cut(s) 370
BspACI CCGC 3 cut(s) 33, 77, 365
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 507
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 3 cut(s) 92, 363, 410
BspMAI CTGCAG 1 cut(s) 290
BspQI GCTCTTC 1 cut(s) 55
BspT107I GGYRCC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 108
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 1 cut(s) 370
BssT1I CCWWGG 1 cut(s) 72
Bst4CI ACNGT 2 cut(s) 385, 404
Bst6I CTCTTC 1 cut(s) 55
BstAUI TGTACA 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 1 cut(s) 494
BstF5I GGATG 2 cut(s) 258, 451
BstKTI GATC 1 cut(s) 373
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstSFI CTRYAG 1 cut(s) 286
BstSLI GKGCMC 1 cut(s) 314
BstV1I GCAGC 1 cut(s) 300
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 1 cut(s) 71
BtsCI GGATG 2 cut(s) 258, 451
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 1 cut(s) 217
CciI TCATGA 1 cut(s) 451
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 259
CviAII CATG 4 cut(s) 236, 331, 452, 466
CviJI RGCY 6 cut(s) 5, 71, 115, 199, 281, 491
CviKI_1 RGCY 6 cut(s) 5, 71, 115, 199, 281, 491
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 494
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 239
Eco57I CTGAAG 2 cut(s) 378, 399
Eco81I CCTNAGG 1 cut(s) 494
EcoRI GAATTC 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 4 cut(s) 239, 334, 455, 469
FatI CATG 4 cut(s) 235, 330, 451, 465
FauNDI CATATG 1 cut(s) 211
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 2 cut(s) 289, 365
FokI GGATG 2 cut(s) 265, 458
Fsp4HI GCNGC 2 cut(s) 289, 365
FspBI CTAG 1 cut(s) 353
GluI GCNGC 2 cut(s) 289, 365
GsuI CTGGAG 1 cut(s) 165
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 123
Hin1II CATG 4 cut(s) 239, 334, 455, 469
HinfI GANTC 1 cut(s) 499
HpaII CCGG 1 cut(s) 123
Hpy188I TCNGA 2 cut(s) 268, 418
Hpy188III TCNNGA 3 cut(s) 88, 452, 496
HpyAV CCTTC 3 cut(s) 292, 402, 423
HpyCH4III ACNGT 2 cut(s) 385, 404
HpyCH4V TGCA 2 cut(s) 288, 334
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 1 cut(s) 494
Hsp92II CATG 4 cut(s) 239, 334, 455, 469
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 1 cut(s) 370
LguI GCTCTTC 1 cut(s) 55
Lsp1109I GCAGC 1 cut(s) 300
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 436
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 2 cut(s) 72, 164
MhlI GDGCHC 1 cut(s) 314
MluCI AATT 1 cut(s) 455
MmeI TCCRAC 2 cut(s) 347, 386
MnlI CCTC 4 cut(s) 10, 61, 140, 503
Mph1103I ATGCAT 1 cut(s) 336
MseI TTAA 1 cut(s) 377
MslI CAYNNNNRTG 1 cut(s) 210
MspI CCGG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeI CATATG 1 cut(s) 211
NdeII GATC 1 cut(s) 370
NlaIII CATG 4 cut(s) 239, 334, 455, 469
NlaIV GGNNCC 3 cut(s) 92, 363, 410
NmuCI GTSAC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 336
PagI TCATGA 1 cut(s) 451
PciSI GCTCTTC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 499
PkrI GCNGC 2 cut(s) 290, 366
PspN4I GGNNCC 3 cut(s) 92, 363, 410
PspPI GGNCC 1 cut(s) 239
PstI CTGCAG 1 cut(s) 290
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
RseI CAYNNNNRTG 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 377
SatI GCNGC 2 cut(s) 289, 365
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 1 cut(s) 239
SduI GDGCHC 1 cut(s) 314
SetI ASST 4 cut(s) 135, 201, 244, 349
SfcI CTRYAG 1 cut(s) 286
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 210
Sse9I AATT 1 cut(s) 455
SsiI CCGC 3 cut(s) 33, 77, 365
SspMI CTAG 1 cut(s) 353
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 385, 404
TasI AATT 1 cut(s) 455
TatI WGTACW 1 cut(s) 258
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 1 cut(s) 377
Tru9I TTAA 1 cut(s) 377
TscAI CASTG 1 cut(s) 35
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 1 cut(s) 288
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 4 cut(s) 198, 454, 463, 468
TspRI CASTG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 1 cut(s) 455
XspI CTAG 1 cut(s) 353
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.