MD10G1155500.v1.1

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008764
Physical Location & Seq
Reverse (-)
24351450 .. 24356401
4952 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1155500.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGCTAATATGGTTGAGGCAATGAGCCAGTGCGGTAAAAGGGTTGAAGTTGCCACCAGAAGAGCAGAGACCATGGCGGACAATGTCTGGAACCACTTTTACAATGTTGGTAGAGCTTCCCTACAGAATTTCGAATTGATTGACCTTTATCGATCTTCATTCGCATGTCATACAAGTAGCCTAGATCATAACAAATTTTGGAATTTTTTCTTGACAGCGAGAATGTGTCCAAGTTTCACAGAAGCAGTGAAAGCAAGGCTAAATACCGGCACAAAGGTGCTTACTGCCGGAGGACAAGAAAAAGTATTCCAGCAGACATTTGAAAGAATTACTGGAGAGAAGCTCTTACATTCATATGCTTGCTATCTATCAACTTCTCATGGACCTGTGATTGGGATGTTGTACATCTCTAATGTACGAATAGCCTTCTGCAGTGATTTCTCACACTATTTGTCCCCAGGACATCCAACTTCCATGCATTACAAGTTAAGAACAGTAAATCCTTCAGCAAACATATGGAACCCTTTAGAAAAGTATATACACATTGTCACAAGAGATAGTCATGAATTCTGGTTCATGGGGTTCATATCCTATGACAAGGCCCTCGGTAATCTTAGCCGAGCTATACAGTTATCTCAGAATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

216

Amino Acids

24.61

Weight (kDa)

9.11

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 102 - 201 3.1e-12 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 33, 77
AcsI RAATTY 4 cut(s) 127, 194, 202, 566
AcuI CTGAAG 1 cut(s) 489
AfaI GTAC 2 cut(s) 404, 417
AfiI CCNNNNNNNGG 1 cut(s) 392
AgsI TTSAA 2 cut(s) 47, 323
AjnI CCWGG 1 cut(s) 457
AleI CACNNNNGTG 1 cut(s) 275
AloI GAACNNNNNNTCC 2 cut(s) 484, 516
AluBI AGCT 3 cut(s) 116, 343, 623
AluI AGCT 3 cut(s) 116, 343, 623
Alw26I GTCTC 1 cut(s) 62
AoxI GGCC 1 cut(s) 600
ApoI RAATTY 4 cut(s) 127, 194, 202, 566
Asp700I GAANNNNTTC 1 cut(s) 206
AspS9I GGNCC 2 cut(s) 383, 601
AsuII TTCGAA 1 cut(s) 132
AvaII GGWCC 1 cut(s) 383
BciT130I CCWGG 1 cut(s) 459
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 1 cut(s) 182
BfmI CTRYAG 2 cut(s) 122, 430
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 1 cut(s) 383
BmgT120I GGNCC 2 cut(s) 383, 601
BmiI GGNNCC 2 cut(s) 92, 521
BmrFI CCNGG 1 cut(s) 459
BplI GAGNNNNNCTC 2 cut(s) 327, 359
BpmI CTGGAG 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 132
Bsa29I ATCGAT 1 cut(s) 151
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 72, 457, 604
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 2 cut(s) 28, 337
Bse3DI GCAATG 1 cut(s) 27
BseBI CCWGG 1 cut(s) 459
BseCI ATCGAT 1 cut(s) 151
BseDI CCNNGG 3 cut(s) 72, 457, 604
BseGI GGATG 2 cut(s) 402, 463
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMI GCAATG 1 cut(s) 27
BseNI ACTGG 2 cut(s) 28, 337
BshFI GGCC 1 cut(s) 602
BshVI ATCGAT 1 cut(s) 151
BsiSI CCGG 2 cut(s) 267, 288
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 439
BsnI GGCC 1 cut(s) 602
Bso31I GGTCTC 1 cut(s) 62
Bsp119I TTCGAA 1 cut(s) 132
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 2 cut(s) 152, 184
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 2 cut(s) 33, 77
BspANI GGCC 1 cut(s) 602
BspDI ATCGAT 1 cut(s) 151
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 92, 521
BspMAI CTGCAG 1 cut(s) 434
BspQI GCTCTTC 1 cut(s) 55
BspT104I TTCGAA 1 cut(s) 132
BspTNI GGTCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 266
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 2 cut(s) 28, 337
BssAI RCCGGY 1 cut(s) 266
BssECI CCNNGG 3 cut(s) 72, 457, 604
BssMI GATC 2 cut(s) 152, 184
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 2 cut(s) 496, 630
Bst6I CTCTTC 1 cut(s) 55
BstAUI TGTACA 1 cut(s) 402
BstBI TTCGAA 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 361
BstDEI CTNAG 2 cut(s) 614, 636
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 402, 463
BstKTI GATC 2 cut(s) 155, 187
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 2 cut(s) 152, 184
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 457
BstSFI CTRYAG 2 cut(s) 122, 430
Bsu15I ATCGAT 1 cut(s) 151
BsuRI GGCC 1 cut(s) 602
BsuTUI ATCGAT 1 cut(s) 151
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 402, 463
BtsI GCAGTG 2 cut(s) 252, 439
BtsIMutI CAGTG 3 cut(s) 35, 252, 439
Cac8I GCNNGC 1 cut(s) 361
CciI TCATGA 1 cut(s) 562
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 383, 601
ClaI ATCGAT 1 cut(s) 151
Csp6I GTAC 2 cut(s) 403, 416
CviAII CATG 6 cut(s) 73, 165, 380, 475, 563, 577
CviQI GTAC 2 cut(s) 403, 416
DdeI CTNAG 2 cut(s) 614, 636
DpnI GATC 2 cut(s) 154, 186
DpnII GATC 2 cut(s) 152, 184
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
EciI GGCGGA 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 489
EcoO109I RGGNCCY 1 cut(s) 601
EcoRI GAATTC 1 cut(s) 566
EcoRII CCWGG 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 6 cut(s) 76, 168, 383, 478, 566, 580
FaqI GGGAC 1 cut(s) 439
FatI CATG 6 cut(s) 72, 164, 379, 474, 562, 576
FauNDI CATATG 2 cut(s) 355, 515
FokI GGATG 2 cut(s) 409, 450
FspBI CTAG 1 cut(s) 182
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 1 cut(s) 602
HapII CCGG 2 cut(s) 267, 288
Hin1II CATG 6 cut(s) 76, 168, 383, 478, 566, 580
HpaII CCGG 2 cut(s) 267, 288
Hpy188I TCNGA 1 cut(s) 639
Hpy188III TCNNGA 3 cut(s) 88, 211, 563
HpyAV CCTTC 2 cut(s) 436, 513
HpyCH4III ACNGT 2 cut(s) 496, 630
HpyCH4V TGCA 2 cut(s) 432, 478
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 2 cut(s) 614, 636
Hsp92II CATG 6 cut(s) 76, 168, 383, 478, 566, 580
Kzo9I GATC 2 cut(s) 152, 184
LguI GCTCTTC 1 cut(s) 55
MaeI CTAG 1 cut(s) 182
MaeIII GTNAC 1 cut(s) 547
MalI GATC 2 cut(s) 154, 186
MboI GATC 2 cut(s) 152, 184
MboII GAAGA 2 cut(s) 72, 147
MluCI AATT 6 cut(s) 127, 134, 194, 202, 327, 566
MmeI TCCRAC 1 cut(s) 491
MnlI CCTC 3 cut(s) 10, 284, 614
Mph1103I ATGCAT 1 cut(s) 480
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 1 cut(s) 488
MslI CAYNNNNRTG 3 cut(s) 163, 275, 354
MspI CCGG 2 cut(s) 267, 288
MspR9I CCNGG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 1 cut(s) 251
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 2 cut(s) 355, 515
NdeII GATC 2 cut(s) 152, 184
NlaIII CATG 6 cut(s) 76, 168, 383, 478, 566, 580
NlaIV GGNNCC 2 cut(s) 92, 521
NmeAIII GCCGAG 1 cut(s) 644
NmuCI GTSAC 1 cut(s) 547
NsiI ATGCAT 1 cut(s) 480
NspI RCATGY 1 cut(s) 168
NspV TTCGAA 1 cut(s) 132
OliI CACNNNNGTG 1 cut(s) 275
PagI TCATGA 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 206
PflFI GACNNNGTC 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 2 cut(s) 92, 521
PspPI GGNCC 2 cut(s) 383, 601
PsrI GAACNNNNNNTAC 2 cut(s) 83, 115
PstI CTGCAG 1 cut(s) 434
PsyI GACNNNGTC 1 cut(s) 83
RsaI GTAC 2 cut(s) 404, 417
RsaNI GTAC 2 cut(s) 403, 416
RseI CAYNNNNRTG 3 cut(s) 163, 275, 354
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 488
Sau3AI GATC 2 cut(s) 152, 184
Sau96I GGNCC 2 cut(s) 383, 601
ScrFI CCNGG 1 cut(s) 459
SetI ASST 6 cut(s) 118, 147, 279, 345, 388, 625
SfcI CTRYAG 2 cut(s) 122, 430
SfuI TTCGAA 1 cut(s) 132
SinI GGWCC 1 cut(s) 383
SmiMI CAYNNNNRTG 3 cut(s) 163, 275, 354
Sse9I AATT 6 cut(s) 127, 134, 194, 202, 327, 566
SsiI CCGC 2 cut(s) 33, 77
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 457
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 496, 630
TaqI TCGA 2 cut(s) 132, 151
TasI AATT 6 cut(s) 127, 134, 194, 202, 327, 566
TatI WGTACW 1 cut(s) 402
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TscAI CASTG 3 cut(s) 35, 252, 439
TseFI GTSAC 1 cut(s) 547
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 5 cut(s) 147, 342, 565, 574, 579
TspRI CASTG 3 cut(s) 35, 252, 439
Tth111I GACNNNGTC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 4 cut(s) 127, 194, 202, 566
XceI RCATGY 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 1 cut(s) 182
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.