pycom05g15410

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008764
Physical Location & Seq
Reverse (-)
18936438 .. 18937689
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g15410.1

Sequence Viewer

Length: 681 bp
ATGGATAGTCGTCCCTCTGAAACTCAAGGCACAGCCCAGGCAAACAATCCTTATGTCCATTATTCGCCTGTTCCGGCCACATCGAATTCTTCTACCAAGCAACCGATGGCAAATATGGTTGAGGCATTGTGCCACTGCGGTAAAAGGGTTGAAGTTGCCACCAAAAGAGCAGAGGCCAAGGCGGACAATGTCTGGAACCACCTGAGAACGTGCCCGAGTTTCACAGAAGCAATGATGGCAAGGCTAAGTACCGGAACAAAGGTGCTTGCTGCCGGAGGAGAAGAAAAAGTGTTTCAGCAGACATTTGAAAGAGTGACAGGAGAAAAGCTCTTACATTCATATGCTTGCCATCTATCAACTTCTCATGGACCTGTGATTGGGATGATGTACATCTCCGATAAACGAATAGCCTTCTGCAGCGATTTCTCTTACTATTTGTGCCCAGGAAATCCAACTCTCATGCATTACAAGGTAGATATTCTAGTGGACATAATCGAATTCTTAAGTTCTTCATTCCAGCTAAGAACAGTAAATCCTTCAGCAAACAGATGGAACCCTTCGGAAAAATATATACAGATTGTCACAAGGGATGGTCATGAATTCTGGTTCATGGGGTTCATATCCTATGACAAAGCCCTCAGGAATCTAAGCCAAGCTCCAGAGCTATCTCAGAATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

227

Amino Acids

25.39

Weight (kDa)

7.66

Isoelectric Point (pI)

56.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 97 - 212 1.8e-11 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 138, 182
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 3 cut(s) 85, 497, 599
AcuI CTGAAG 1 cut(s) 522
AfaI GTAC 2 cut(s) 250, 389
AfiI CCNNNNNNNGG 1 cut(s) 377
AflII CTTAAG 1 cut(s) 502
AgsI TTSAA 2 cut(s) 152, 308
AjnI CCWGG 2 cut(s) 36, 442
AloI GAACNNNNNNTCC 2 cut(s) 517, 549
AluBI AGCT 4 cut(s) 328, 520, 656, 664
AluI AGCT 4 cut(s) 328, 520, 656, 664
Ama87I CYCGRG 1 cut(s) 214
AoxI GGCC 2 cut(s) 75, 174
ApeKI GCWGC 2 cut(s) 269, 417
ApoI RAATTY 3 cut(s) 85, 497, 599
AspS9I GGNCC 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 214
AvaII GGWCC 1 cut(s) 368
AxyI CCTNAGG 1 cut(s) 638
BaeGI GKGCMC 2 cut(s) 215, 443
BbvI GCAGC 2 cut(s) 256, 429
BccI CCATC 5 cut(s) 100, 229, 357, 543, 584
BciT130I CCWGG 2 cut(s) 38, 444
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 1 cut(s) 415
BfrI CTTAAG 1 cut(s) 502
BisI GCNGC 2 cut(s) 270, 418
BlsI GCNGC 2 cut(s) 271, 419
Bme1390I CCNGG 2 cut(s) 38, 444
Bme18I GGWCC 1 cut(s) 368
BmeT110I CYCGRG 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 2 cut(s) 197, 554
BmrFI CCNGG 2 cut(s) 38, 444
BplI GAGNNNNNCTC 2 cut(s) 312, 344
BpmI CTGGAG 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 9
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 3 cut(s) 36, 177, 442
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse21I CCTNAGG 1 cut(s) 638
Bse3DI GCAATG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 2 cut(s) 38, 444
BseDI CCNNGG 3 cut(s) 36, 177, 442
BseGI GGATG 2 cut(s) 387, 595
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 2 cut(s) 194, 652
BseRI GAGGAG 1 cut(s) 291
BseSI GKGCMC 2 cut(s) 215, 443
BseXI GCAGC 2 cut(s) 256, 429
BshFI GGCC 2 cut(s) 77, 176
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 3 cut(s) 74, 252, 273
BslI CCNNNNNNNGG 1 cut(s) 377
BsnI GGCC 2 cut(s) 77, 176
BsoBI CYCGRG 1 cut(s) 214
Bsp1286I GDGCHC 2 cut(s) 215, 443
Bsp1407I TGTACA 1 cut(s) 387
BspACI CCGC 2 cut(s) 138, 182
BspANI GGCC 2 cut(s) 77, 176
BspCNI CTCAG 2 cut(s) 195, 651
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 197, 554
BspMAI CTGCAG 1 cut(s) 419
BspTI CTTAAG 1 cut(s) 502
BsrDI GCAATG 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 387
BssECI CCNNGG 3 cut(s) 36, 177, 442
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 2 cut(s) 38, 444
Bst4CI ACNGT 1 cut(s) 529
BstAFI CTTAAG 1 cut(s) 502
BstAUI TGTACA 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 267, 346
BstDEI CTNAG 6 cut(s) 203, 245, 521, 638, 647, 669
BstF5I GGATG 2 cut(s) 387, 595
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 2 cut(s) 38, 444
BstSCI CCNGG 2 cut(s) 36, 442
BstSFI CTRYAG 1 cut(s) 415
BstSLI GKGCMC 2 cut(s) 215, 443
BstV1I GCAGC 2 cut(s) 256, 429
Bsu36I CCTNAGG 1 cut(s) 638
BsuRI GGCC 2 cut(s) 77, 176
BtsCI GGATG 2 cut(s) 387, 595
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 267, 346
CciI TCATGA 1 cut(s) 595
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 249, 388
CviAII CATG 4 cut(s) 365, 460, 596, 610
CviQI GTAC 2 cut(s) 249, 388
DdeI CTNAG 6 cut(s) 203, 245, 521, 638, 647, 669
EaeI YGGCCR 1 cut(s) 75
EciI GGCGGA 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 522
Eco81I CCTNAGG 1 cut(s) 638
Eco88I CYCGRG 1 cut(s) 214
EcoRI GAATTC 3 cut(s) 85, 497, 599
EcoRII CCWGG 2 cut(s) 36, 442
EcoT14I CCWWGG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 4 cut(s) 368, 463, 599, 613
FatI CATG 4 cut(s) 364, 459, 595, 609
FauNDI CATATG 1 cut(s) 340
Fnu4HI GCNGC 2 cut(s) 270, 418
FokI GGATG 2 cut(s) 394, 602
Fsp4HI GCNGC 2 cut(s) 270, 418
FspBI CTAG 1 cut(s) 482
GluI GCNGC 2 cut(s) 270, 418
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 2 cut(s) 77, 176
HapII CCGG 3 cut(s) 74, 252, 273
Hin1II CATG 4 cut(s) 368, 463, 599, 613
HinfI GANTC 1 cut(s) 643
HpaII CCGG 3 cut(s) 74, 252, 273
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 4 cut(s) 19, 397, 562, 672
Hpy188III TCNNGA 4 cut(s) 193, 596, 640, 659
Hpy8I GTNNAC 1 cut(s) 487
HpyAV CCTTC 3 cut(s) 421, 546, 567
HpyCH4III ACNGT 1 cut(s) 529
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 2 cut(s) 417, 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 6 cut(s) 203, 245, 521, 638, 647, 669
HpySE526I ACGT 1 cut(s) 209
Hsp92II CATG 4 cut(s) 368, 463, 599, 613
LmnI GCTCC 1 cut(s) 661
Lsp1109I GCAGC 2 cut(s) 256, 429
MaeI CTAG 1 cut(s) 482
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 2 cut(s) 313, 580
MboII GAAGA 3 cut(s) 81, 293, 501
MhlI GDGCHC 2 cut(s) 215, 443
MluCI AATT 3 cut(s) 85, 497, 599
MmeI TCCRAC 1 cut(s) 476
MnlI CCTC 5 cut(s) 25, 115, 166, 269, 647
Mph1103I ATGCAT 1 cut(s) 465
MseI TTAA 1 cut(s) 503
MslI CAYNNNNRTG 1 cut(s) 339
MspCI CTTAAG 1 cut(s) 502
MspI CCGG 3 cut(s) 74, 252, 273
MspR9I CCNGG 2 cut(s) 38, 444
MvaI CCWGG 2 cut(s) 38, 444
MwoI GCNNNNNNNGC 1 cut(s) 236
NdeI CATATG 1 cut(s) 340
NlaIII CATG 4 cut(s) 368, 463, 599, 613
NlaIV GGNNCC 2 cut(s) 197, 554
NmuCI GTSAC 2 cut(s) 313, 580
NsiI ATGCAT 1 cut(s) 465
PagI TCATGA 1 cut(s) 595
PfeI GAWTC 1 cut(s) 643
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 2 cut(s) 271, 419
Psp6I CCWGG 2 cut(s) 36, 442
PspGI CCWGG 2 cut(s) 36, 442
PspN4I GGNNCC 2 cut(s) 197, 554
PspPI GGNCC 1 cut(s) 368
PstI CTGCAG 1 cut(s) 419
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 2 cut(s) 250, 389
RsaNI GTAC 2 cut(s) 249, 388
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 503
SatI GCNGC 2 cut(s) 270, 418
Sau96I GGNCC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 38, 444
SduI GDGCHC 2 cut(s) 215, 443
SetI ASST 9 cut(s) 204, 212, 264, 330, 373, 474, 522, 658, 666
SfcI CTRYAG 1 cut(s) 415
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 2 cut(s) 24, 502
SmoI CTYRAG 2 cut(s) 24, 502
Sse9I AATT 3 cut(s) 85, 497, 599
SsiI CCGC 2 cut(s) 138, 182
SspMI CTAG 1 cut(s) 482
StyD4I CCNGG 2 cut(s) 36, 442
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 529
TaiI ACGT 1 cut(s) 212
TaqI TCGA 2 cut(s) 83, 495
TasI AATT 3 cut(s) 85, 497, 599
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 1 cut(s) 503
Tru9I TTAA 1 cut(s) 503
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 2 cut(s) 313, 580
TseI GCWGC 2 cut(s) 269, 417
Tsp45I GTSAC 2 cut(s) 313, 580
TspDTI ATGAA 5 cut(s) 327, 501, 598, 607, 612
TspRI CASTG 1 cut(s) 140
Tth111I GACNNNGTC 1 cut(s) 188
Vha464I CTTAAG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 3 cut(s) 85, 497, 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XspI CTAG 1 cut(s) 482
Zsp2I ATGCAT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.