FvH4_2g18080

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008764
Physical Location & Seq
Reverse (-)
15481811 .. 15484488
2678 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g18080.t1

Sequence Viewer

Length: 705 bp
ATGAAGGCTAGAAAAAACAATTCTAACAACATGAACATGAAGATGAATGGTAGTCCTCCTCGAATTGAAGGCCAAGCCTACACCAAACTCGACCACAACAATCCTTCTGCTCAATGTTCCCCAGTTCCCACATCGAATTCACCTGGAAAACGTCCCATAGACAAGGTGCTTGACACATTGAATCATTGCGGTAAAAGAGCAGACACCATTACAGATAATGTCTGGAATCACTTGAGAATGAGTCCTAGTCTTGCAGACGCTGCAATGGCAAGGCTTACTCATGGGACAAAGGCTCTTACAAGAGGGGATGAAAAAGTATTCCAGCAAACATTTGAGACTGTCATGCTACATGAGAAGCTCCTGCATTCATATGCTTGCTATCTCTCAACTTCTACTGGACCTGTGATTGGAATGCTGTATATTTCCAATAAAAGAGTAGCCTTTTGTAGTGATTTCTGTCACTATATCTCCCCAACGCAGCCAAACACTTACTACAAGGTGGTGGTGCAGCTTGATCAGTTAAGAGCAGTGAATCCTTCTGCAAACAGATGGAACCCTTCAGAGAAATACATACAGATAGTCACAATAGACGGTCATGAGTTCTGGTTCATGGGGTTCATATCTTATGACAAGGCCCTCACTAATCTAACCCAAGCTCTAAACCCTACTTCTGGTGGAACTCCTCTACTAAATAACTTCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

235

Amino Acids

26.22

Weight (kDa)

9.18

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 106 - 220 1.2e-15 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 189
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 2 cut(s) 543, 682
AfiI CCNNNNNNNGG 2 cut(s) 407, 671
AgsI TTSAA 2 cut(s) 68, 181
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 3 cut(s) 358, 511, 656
AluI AGCT 3 cut(s) 358, 511, 656
Alw26I GTCTC 1 cut(s) 329
AlwNI CAGNNNCTG 1 cut(s) 260
AoxI GGCC 2 cut(s) 70, 633
ApeKI GCWGC 3 cut(s) 260, 478, 508
ApoI RAATTY 1 cut(s) 136
AspS9I GGNCC 2 cut(s) 398, 634
AsuHPI GGTGA 1 cut(s) 132
AvaII GGWCC 1 cut(s) 398
BbvI GCAGC 3 cut(s) 247, 490, 520
BccI CCATC 1 cut(s) 543
BciT130I CCWGG 1 cut(s) 144
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 2 cut(s) 9, 246
BisI GCNGC 3 cut(s) 261, 479, 509
BlsI GCNGC 3 cut(s) 262, 480, 510
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 2 cut(s) 398, 634
BmiI GGNNCC 1 cut(s) 554
BmrFI CCNGG 1 cut(s) 144
BmrI ACTGGG 1 cut(s) 116
BmuI ACTGGG 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 253
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 671
Bse1I ACTGG 2 cut(s) 122, 400
Bse3DI GCAATG 2 cut(s) 184, 270
BseBI CCWGG 1 cut(s) 144
BseGI GGATG 1 cut(s) 313
BseLI CCNNNNNNNGG 2 cut(s) 407, 671
BseMI GCAATG 2 cut(s) 184, 270
BseNI ACTGG 2 cut(s) 122, 400
BseRI GAGGAG 2 cut(s) 48, 672
BseXI GCAGC 3 cut(s) 247, 490, 520
BsgI GTGCAG 1 cut(s) 527
BshFI GGCC 2 cut(s) 72, 635
BslFI GGGAC 2 cut(s) 138, 298
BslI CCNNNNNNNGG 2 cut(s) 407, 671
BsmAI GTCTC 1 cut(s) 329
BsmFI GGGAC 2 cut(s) 138, 298
BsmI GAATGC 2 cut(s) 364, 417
BsnI GGCC 2 cut(s) 72, 635
Bsp143I GATC 1 cut(s) 514
BspACI CCGC 1 cut(s) 189
BspANI GGCC 2 cut(s) 72, 635
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 1 cut(s) 554
BsrDI GCAATG 2 cut(s) 184, 270
BsrI ACTGG 2 cut(s) 122, 400
BssMI GATC 1 cut(s) 514
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 2 cut(s) 340, 593
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 376
BstF5I GGATG 1 cut(s) 313
BstKTI GATC 1 cut(s) 517
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 1 cut(s) 514
BstMWI GCNNNNNNNGC 2 cut(s) 260, 266
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstV1I GCAGC 3 cut(s) 247, 490, 520
BsuRI GGCC 2 cut(s) 72, 635
BtsCI GGATG 1 cut(s) 313
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 1 cut(s) 376
CaiI CAGNNNCTG 1 cut(s) 260
CciI TCATGA 1 cut(s) 595
Cfr13I GGNCC 2 cut(s) 398, 634
CseI GACGC 1 cut(s) 266
CviAII CATG 7 cut(s) 31, 37, 281, 343, 350, 596, 610
DpnI GATC 1 cut(s) 516
DpnII GATC 1 cut(s) 514
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 2 cut(s) 543, 682
EcoO109I RGGNCCY 1 cut(s) 634
EcoRI GAATTC 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 142
FaeI CATG 7 cut(s) 34, 40, 284, 346, 353, 599, 613
FaqI GGGAC 2 cut(s) 138, 298
FatI CATG 7 cut(s) 30, 36, 280, 342, 349, 595, 609
FauNDI CATATG 1 cut(s) 370
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 3 cut(s) 261, 479, 509
FokI GGATG 1 cut(s) 320
Fsp4HI GCNGC 3 cut(s) 261, 479, 509
FspBI CTAG 2 cut(s) 9, 246
GluI GCNGC 3 cut(s) 261, 479, 509
HaeIII GGCC 2 cut(s) 72, 635
HgaI GACGC 1 cut(s) 266
Hin1II CATG 7 cut(s) 34, 40, 284, 346, 353, 599, 613
HinfI GANTC 4 cut(s) 181, 226, 241, 532
HphI GGTGA 1 cut(s) 132
Hpy188I TCNGA 1 cut(s) 562
Hpy188III TCNNGA 2 cut(s) 223, 596
HpyAV CCTTC 4 cut(s) 62, 114, 546, 567
HpyCH4III ACNGT 2 cut(s) 340, 593
HpyCH4IV ACGT 1 cut(s) 151
HpyCH4V TGCA 5 cut(s) 254, 263, 364, 508, 542
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 266
HpySE526I ACGT 1 cut(s) 151
Hsp92II CATG 7 cut(s) 34, 40, 284, 346, 353, 599, 613
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 1 cut(s) 514
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 3 cut(s) 247, 490, 520
MaeI CTAG 2 cut(s) 9, 246
MaeII ACGT 1 cut(s) 151
MaeIII GTNAC 2 cut(s) 458, 580
MalI GATC 1 cut(s) 516
MboI GATC 1 cut(s) 514
MboII GAAGA 1 cut(s) 52
MluCI AATT 3 cut(s) 19, 63, 136
MlyI GAGTC 1 cut(s) 250
MnlI CCTC 5 cut(s) 66, 69, 296, 647, 693
MseI TTAA 2 cut(s) 521, 703
MslI CAYNNNNRTG 3 cut(s) 35, 41, 369
MspR9I CCNGG 1 cut(s) 144
Mva1269I GAATGC 2 cut(s) 364, 417
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 2 cut(s) 260, 266
NdeI CATATG 1 cut(s) 370
NdeII GATC 1 cut(s) 514
NlaIII CATG 7 cut(s) 34, 40, 284, 346, 353, 599, 613
NlaIV GGNNCC 1 cut(s) 554
NmuCI GTSAC 2 cut(s) 458, 580
PagI TCATGA 1 cut(s) 595
PctI GAATGC 2 cut(s) 364, 417
PfeI GAWTC 3 cut(s) 181, 226, 532
PkrI GCNGC 3 cut(s) 262, 480, 510
PleI GAGTC 1 cut(s) 249
PpsI GAGTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 554
PspPI GGNCC 2 cut(s) 398, 634
PstNI CAGNNNCTG 1 cut(s) 260
RseI CAYNNNNRTG 3 cut(s) 35, 41, 369
SaqAI TTAA 2 cut(s) 521, 703
SatI GCNGC 3 cut(s) 261, 479, 509
Sau3AI GATC 1 cut(s) 514
Sau96I GGNCC 2 cut(s) 398, 634
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 1 cut(s) 144
SetI ASST 8 cut(s) 145, 154, 168, 360, 403, 501, 513, 658
SinI GGWCC 1 cut(s) 398
SmiMI CAYNNNNRTG 3 cut(s) 35, 41, 369
SmlI CTYRAG 1 cut(s) 232
SmoI CTYRAG 1 cut(s) 232
Sse9I AATT 3 cut(s) 19, 63, 136
SsiI CCGC 1 cut(s) 189
SspMI CTAG 2 cut(s) 9, 246
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 2 cut(s) 340, 593
TaiI ACGT 1 cut(s) 154
TaqI TCGA 3 cut(s) 61, 90, 134
TasI AATT 3 cut(s) 19, 63, 136
TfiI GAWTC 3 cut(s) 181, 226, 532
Tru1I TTAA 2 cut(s) 521, 703
Tru9I TTAA 2 cut(s) 521, 703
TscAI CASTG 1 cut(s) 534
TseFI GTSAC 2 cut(s) 458, 580
TseI GCWGC 3 cut(s) 260, 478, 508
Tsp45I GTSAC 2 cut(s) 458, 580
TspDTI ATGAA 8 cut(s) 17, 47, 53, 59, 324, 357, 598, 607
TspRI CASTG 1 cut(s) 534
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 1 cut(s) 136
XspI CTAG 2 cut(s) 9, 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.