Rroxscaffold_7G00185510

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008764
Physical Location & Seq
Forward (+)
24911578 .. 24918296
6719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00185510.1

Sequence Viewer

Length: 774 bp
ATGAAGGCTAGAAAAAACAATTCCAACAACATGAACATGATGATGAATGGCAGTCCTCCTCCTCAAATTGAAGGCAATGCCTACGCCAACTTCAACCACAACAATCCTTATGTTAAATATTCCCCAGTTCCCACGTCGAATTCACCTACCAAGCGTCCCATAGATAAGGTGCTTGACACATTGAATCATTGTGGTAAAAGGGTTGAAGGTGCCTCCAGAAGAGCAGACACCATTACAGACAATGTCTGGCATCACTTGAGAATGAGTCCTAGTCTTGCAGACGCGGCAATGGCGAGGCTTACTCACGGGACAAAGGCTCTTACAAGAGGGGATGATAAAGTATTCCAGCAAAAATTTGAGACTGTGATGCTACAAGAGAAGCTCCTGCATTCATATGCTTGCTATCTCTCAACTTCTACTGGACCTGTAATTGGAGTGCTGTATATTTCCAATAAAAGAATAGCCTTTTGTAGTGATTTCTGTCACTATATTTCCCCAGCGCATCCGAACACATATTTTAAGGTGGTGGTGCAGCTTGATCAGTTAAGAGCCGTGAATGCTTCAGCAAACAGATGGAACCCTTCAGAGAAATACATACAGATAGTAACAAGAGACGGTCATGAGTTCTGGTTCATGGGGTTCATATCTTATGACAAGGCCCTCAGTAACCTAACCCAAGCTCTCAACCCTAGTTCTGATGGAACCAATCCTTTAGAAAAGTCCATGAGTACTGCTCGGGAATCTAGTACTACTGGAACTCATTCTCAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

257

Amino Acids

28.83

Weight (kDa)

9.32

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 114 - 228 9.9e-17 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 209
AccII CGCG 1 cut(s) 284
AciI CCGC 1 cut(s) 284
AcsI RAATTY 2 cut(s) 139, 353
AcuI CTGAAG 2 cut(s) 546, 567
AfaI GTAC 2 cut(s) 730, 748
AfiI CCNNNNNNNGG 1 cut(s) 431
AgsI TTSAA 4 cut(s) 71, 94, 184, 206
AjiI CACGTC 1 cut(s) 135
AluBI AGCT 3 cut(s) 382, 535, 680
AluI AGCT 3 cut(s) 382, 535, 680
Alw26I GTCTC 2 cut(s) 353, 606
Ama87I CYCGRG 1 cut(s) 735
AoxI GGCC 1 cut(s) 657
ApeKI GCWGC 1 cut(s) 532
ApoI RAATTY 2 cut(s) 139, 353
Asp700I GAANNNNTTC 1 cut(s) 760
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 2 cut(s) 422, 658
AsuHPI GGTGA 1 cut(s) 135
AvaI CYCGRG 1 cut(s) 735
AvaII GGWCC 1 cut(s) 422
BanI GGYRCC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 544
BccI CCATC 2 cut(s) 567, 692
BceAI ACGGC 1 cut(s) 536
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 2 cut(s) 353, 606
BfaI CTAG 5 cut(s) 9, 270, 690, 744, 772
BisI GCNGC 2 cut(s) 285, 533
BlsI GCNGC 2 cut(s) 286, 534
BmcAI AGTACT 2 cut(s) 730, 748
Bme18I GGWCC 1 cut(s) 422
BmeT110I CYCGRG 1 cut(s) 735
BmgBI CACGTC 1 cut(s) 135
BmgT120I GGNCC 2 cut(s) 422, 658
BmiI GGNNCC 3 cut(s) 211, 578, 703
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 3 cut(s) 259, 357, 511
BmuI ACTGGG 1 cut(s) 119
BplI GAGNNNNNCTC 4 cut(s) 286, 318, 718, 750
BpmI CTGGAG 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 277
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse1I ACTGG 3 cut(s) 125, 424, 757
Bse3DI GCAATG 2 cut(s) 82, 294
BseGI GGATG 2 cut(s) 337, 502
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMI GCAATG 2 cut(s) 82, 294
BseMII CTCAG 1 cut(s) 676
BseNI ACTGG 3 cut(s) 125, 424, 757
BseRI GAGGAG 2 cut(s) 48, 51
BseXI GCAGC 1 cut(s) 544
BseYI CCCAGC 1 cut(s) 496
BsgI GTGCAG 1 cut(s) 551
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 1 cut(s) 659
BshNI GGYRCC 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 735
BslFI GGGAC 2 cut(s) 141, 322
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 2 cut(s) 353, 606
BsmBI CGTCTC 1 cut(s) 606
BsmFI GGGAC 2 cut(s) 141, 322
BsmI GAATGC 2 cut(s) 388, 562
BsnI GGCC 1 cut(s) 659
BsoBI CYCGRG 1 cut(s) 735
Bsp143I GATC 1 cut(s) 538
BspACI CCGC 1 cut(s) 284
BspANI GGCC 1 cut(s) 659
BspCNI CTCAG 1 cut(s) 675
BspFNI CGCG 1 cut(s) 284
BspHI TCATGA 1 cut(s) 619
BspLI GGNNCC 3 cut(s) 211, 578, 703
BspQI GCTCTTC 1 cut(s) 214
BspT107I GGYRCC 1 cut(s) 209
BsrDI GCAATG 2 cut(s) 82, 294
BsrI ACTGG 3 cut(s) 125, 424, 757
BssMI GATC 1 cut(s) 538
Bst4CI ACNGT 2 cut(s) 364, 617
Bst6I CTCTTC 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 400
BstDEI CTNAG 2 cut(s) 662, 765
BstF5I GGATG 2 cut(s) 337, 502
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 1 cut(s) 541
BstMAI GTCTC 2 cut(s) 353, 606
BstMBI GATC 1 cut(s) 538
BstMWI GCNNNNNNNGC 3 cut(s) 284, 290, 557
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 1 cut(s) 544
BsuRI GGCC 1 cut(s) 659
BtrI CACGTC 1 cut(s) 135
BtsCI GGATG 2 cut(s) 337, 502
Cac8I GCNNGC 1 cut(s) 400
CciI TCATGA 1 cut(s) 619
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 2 cut(s) 422, 658
CseI GACGC 2 cut(s) 143, 290
Csp6I GTAC 2 cut(s) 729, 747
CviAII CATG 5 cut(s) 31, 37, 620, 634, 724
CviJI RGCY 9 cut(s) 8, 298, 317, 382, 464, 535, 551, 659, 680
CviKI_1 RGCY 9 cut(s) 8, 298, 317, 382, 464, 535, 551, 659, 680
CviQI GTAC 2 cut(s) 729, 747
DdeI CTNAG 2 cut(s) 662, 765
DpnI GATC 1 cut(s) 540
DpnII GATC 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 214
EarI CTCTTC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 422
Eco57I CTGAAG 2 cut(s) 546, 567
Eco88I CYCGRG 1 cut(s) 735
EcoO109I RGGNCCY 1 cut(s) 658
EcoRI GAATTC 1 cut(s) 139
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 5 cut(s) 34, 40, 623, 637, 727
FaqI GGGAC 2 cut(s) 141, 322
FatI CATG 5 cut(s) 30, 36, 619, 633, 723
FauNDI CATATG 1 cut(s) 394
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 2 cut(s) 285, 533
FokI GGATG 2 cut(s) 344, 489
Fsp4HI GCNGC 2 cut(s) 285, 533
FspBI CTAG 5 cut(s) 9, 270, 690, 744, 772
GlaI GCGC 1 cut(s) 501
GluI GCNGC 2 cut(s) 285, 533
GsaI CCCAGC 1 cut(s) 500
GsuI CTGGAG 1 cut(s) 199
HaeIII GGCC 1 cut(s) 659
HgaI GACGC 2 cut(s) 143, 290
HhaI GCGC 1 cut(s) 502
Hin1II CATG 5 cut(s) 34, 40, 623, 637, 727
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HinfI GANTC 3 cut(s) 184, 265, 740
HphI GGTGA 1 cut(s) 135
Hpy188I TCNGA 3 cut(s) 507, 586, 697
Hpy188III TCNNGA 3 cut(s) 216, 620, 737
Hpy99I CGWCG 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 65, 200, 591
HpyCH4III ACNGT 2 cut(s) 364, 617
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 3 cut(s) 278, 388, 532
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 290, 557
HpyF3I CTNAG 2 cut(s) 662, 765
HpySE526I ACGT 1 cut(s) 134
Hsp92II CATG 5 cut(s) 34, 40, 623, 637, 727
HspAI GCGC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 1 cut(s) 538
LguI GCTCTTC 1 cut(s) 214
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 1 cut(s) 544
LweI GCATC 3 cut(s) 259, 357, 511
MaeI CTAG 5 cut(s) 9, 270, 690, 744, 772
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 3 cut(s) 482, 604, 665
MalI GATC 1 cut(s) 540
MboI GATC 1 cut(s) 538
MboII GAAGA 1 cut(s) 231
MluCI AATT 5 cut(s) 19, 66, 139, 353, 429
MlyI GAGTC 1 cut(s) 274
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 7 cut(s) 66, 69, 72, 223, 288, 320, 671
MroXI GAANNNNTTC 1 cut(s) 760
MseI TTAA 3 cut(s) 114, 519, 545
MslI CAYNNNNRTG 3 cut(s) 35, 41, 393
Mva1269I GAATGC 2 cut(s) 388, 562
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 3 cut(s) 284, 290, 557
NdeI CATATG 1 cut(s) 394
NdeII GATC 1 cut(s) 538
NlaIII CATG 5 cut(s) 34, 40, 623, 637, 727
NlaIV GGNNCC 3 cut(s) 211, 578, 703
NmuCI GTSAC 1 cut(s) 482
PagI TCATGA 1 cut(s) 619
PciSI GCTCTTC 1 cut(s) 214
PctI GAATGC 2 cut(s) 388, 562
PdmI GAANNNNTTC 1 cut(s) 760
PfeI GAWTC 2 cut(s) 184, 740
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 2 cut(s) 286, 534
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
PspFI CCCAGC 1 cut(s) 496
PspN4I GGNNCC 3 cut(s) 211, 578, 703
PspPI GGNCC 2 cut(s) 422, 658
PsyI GACNNNGTC 1 cut(s) 242
RsaI GTAC 2 cut(s) 730, 748
RsaNI GTAC 2 cut(s) 729, 747
RseI CAYNNNNRTG 3 cut(s) 35, 41, 393
SapI GCTCTTC 1 cut(s) 214
SaqAI TTAA 3 cut(s) 114, 519, 545
SatI GCNGC 2 cut(s) 285, 533
Sau3AI GATC 1 cut(s) 538
Sau96I GGNCC 2 cut(s) 422, 658
ScaI AGTACT 2 cut(s) 730, 748
SchI GAGTC 1 cut(s) 274
SfaNI GCATC 3 cut(s) 259, 357, 511
SinI GGWCC 1 cut(s) 422
SmiMI CAYNNNNRTG 3 cut(s) 35, 41, 393
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 5 cut(s) 19, 66, 139, 353, 429
SsiI CCGC 1 cut(s) 284
SspI AATATT 1 cut(s) 119
SspMI CTAG 5 cut(s) 9, 270, 690, 744, 772
TaaI ACNGT 2 cut(s) 364, 617
TaiI ACGT 1 cut(s) 137
TaqI TCGA 1 cut(s) 137
TasI AATT 5 cut(s) 19, 66, 139, 353, 429
TatI WGTACW 2 cut(s) 728, 746
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 2 cut(s) 184, 740
Tru1I TTAA 3 cut(s) 114, 519, 545
Tru9I TTAA 3 cut(s) 114, 519, 545
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 1 cut(s) 532
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 6 cut(s) 17, 47, 59, 381, 622, 631
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 422
XapI RAATTY 2 cut(s) 139, 353
XmnI GAANNNNTTC 1 cut(s) 760
XspI CTAG 5 cut(s) 9, 270, 690, 744, 772
ZrmI AGTACT 2 cut(s) 730, 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.