Rh6DG262700

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008764
Physical Location & Seq
Reverse (-)
45421681 .. 45426274
4594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG262700.1

Sequence Viewer

Length: 612 bp
ATGATGTTGGTTGTTTTCGTTTTTTTTTTTCATTTTGGTCCAGGTCCCATAGATAAGGTGCTTGACACATTGAATCATTGTGGTAAAAGGGTTGAAGGTGCCTCCAGAAGAGCAGACACCATTACAGACAATGTCTGGCATCACTTGAGAATGAGTCCTAGTCTTGCAGACGCGGCAATGGCAAGGCTTACTCACGGGACAAAGGCTCTTACAAGAGGGGATGATAAAGTATTCCAGCAAAAATTTGAGACTGTGATGCTACAAGAGAAGCTCCTGCATTCATATGCTTGCTATCTCTCAACTTCTACTGGACCTGTAATTGGAGTGCTGTATATTTCCAATAAAAGAATAGCCTTTTGTAGTGATTTCTGTCACTATATTTCCCCAGCGCATCCGAACACATATTTTAAGGTGGTGGTGCAGCTTGATCAGTTAAGATCAGTGAATCCTTCTGCAAACAGATGGAATCCTTCAGAGAAATACATACAGATAGTAACAAGAGATGGTCATGAGTTCTGGTTCATGGGGTTCATATCATATGACAAGGCCCTCAGTAACCTAACCCAAGCTCTCAACCCTAGTTCTGGTGGAACTCATCTCAACCCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

203

Amino Acids

22.95

Weight (kDa)

9.06

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 77 - 191 2.7e-17 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AccII CGCG 1 cut(s) 173
AciI CCGC 1 cut(s) 173
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 456
AfiI CCNNNNNNNGG 2 cut(s) 320, 584
AgsI TTSAA 2 cut(s) 73, 95
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 3 cut(s) 271, 424, 569
AluI AGCT 3 cut(s) 271, 424, 569
Alw26I GTCTC 1 cut(s) 242
AoxI GGCC 1 cut(s) 546
ApeKI GCWGC 1 cut(s) 421
ApoI RAATTY 1 cut(s) 242
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 4 cut(s) 38, 44, 311, 547
AvaII GGWCC 3 cut(s) 38, 44, 311
BanI GGYRCC 1 cut(s) 98
BbvI GCAGC 1 cut(s) 433
BccI CCATC 2 cut(s) 456, 497
BciT130I CCWGG 1 cut(s) 42
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 2 cut(s) 159, 579
BisI GCNGC 2 cut(s) 174, 422
BlsI GCNGC 2 cut(s) 175, 423
Bme1390I CCNGG 1 cut(s) 42
Bme18I GGWCC 3 cut(s) 38, 44, 311
BmgT120I GGNCC 4 cut(s) 38, 44, 311, 547
BmiI GGNNCC 2 cut(s) 46, 100
BmrFI CCNGG 1 cut(s) 42
BmsI GCATC 3 cut(s) 148, 246, 400
BpmI CTGGAG 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 166
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 584
Bse1I ACTGG 1 cut(s) 313
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 1 cut(s) 42
BseGI GGATG 2 cut(s) 226, 391
BseLI CCNNNNNNNGG 2 cut(s) 320, 584
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 1 cut(s) 565
BseNI ACTGG 1 cut(s) 313
BseXI GCAGC 1 cut(s) 433
BseYI CCCAGC 1 cut(s) 385
BsgI GTGCAG 1 cut(s) 440
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 1 cut(s) 548
BshNI GGYRCC 1 cut(s) 98
BslFI GGGAC 2 cut(s) 30, 211
BslI CCNNNNNNNGG 2 cut(s) 320, 584
BsmAI GTCTC 1 cut(s) 242
BsmFI GGGAC 2 cut(s) 30, 211
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 1 cut(s) 548
Bsp143I GATC 2 cut(s) 427, 437
BspACI CCGC 1 cut(s) 173
BspANI GGCC 1 cut(s) 548
BspCNI CTCAG 1 cut(s) 564
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 46, 100
BspQI GCTCTTC 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 1 cut(s) 313
BssMI GATC 2 cut(s) 427, 437
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 1 cut(s) 551
BstF5I GGATG 2 cut(s) 226, 391
BstFNI CGCG 1 cut(s) 173
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 2 cut(s) 430, 440
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 2 cut(s) 427, 437
BstMWI GCNNNNNNNGC 2 cut(s) 173, 179
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 40
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 1 cut(s) 433
BsuRI GGCC 1 cut(s) 548
BtsCI GGATG 2 cut(s) 226, 391
BtsIMutI CAGTG 1 cut(s) 447
Cac8I GCNNGC 1 cut(s) 289
CciI TCATGA 1 cut(s) 508
CfoI GCGC 1 cut(s) 391
Cfr13I GGNCC 4 cut(s) 38, 44, 311, 547
CseI GACGC 1 cut(s) 179
CviAII CATG 2 cut(s) 509, 523
CviJI RGCY 7 cut(s) 187, 206, 271, 353, 424, 548, 569
CviKI_1 RGCY 7 cut(s) 187, 206, 271, 353, 424, 548, 569
DdeI CTNAG 1 cut(s) 551
DpnI GATC 2 cut(s) 429, 439
DpnII GATC 2 cut(s) 427, 437
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Eco47I GGWCC 3 cut(s) 38, 44, 311
Eco57I CTGAAG 1 cut(s) 456
EcoO109I RGGNCCY 2 cut(s) 44, 547
EcoRII CCWGG 1 cut(s) 40
FaeI CATG 2 cut(s) 512, 526
FaqI GGGAC 2 cut(s) 30, 211
FatI CATG 2 cut(s) 508, 522
FauNDI CATATG 2 cut(s) 283, 538
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 2 cut(s) 174, 422
FokI GGATG 2 cut(s) 233, 378
Fsp4HI GCNGC 2 cut(s) 174, 422
FspBI CTAG 2 cut(s) 159, 579
GlaI GCGC 1 cut(s) 390
GluI GCNGC 2 cut(s) 174, 422
GsaI CCCAGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 88
HaeIII GGCC 1 cut(s) 548
HgaI GACGC 1 cut(s) 179
HhaI GCGC 1 cut(s) 391
Hin1II CATG 2 cut(s) 512, 526
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HinfI GANTC 4 cut(s) 73, 154, 445, 466
Hpy188I TCNGA 2 cut(s) 396, 475
Hpy188III TCNNGA 2 cut(s) 105, 509
HpyAV CCTTC 3 cut(s) 89, 459, 480
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4V TGCA 4 cut(s) 167, 277, 421, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 179
HpyF3I CTNAG 1 cut(s) 551
Hsp92II CATG 2 cut(s) 512, 526
HspAI GCGC 1 cut(s) 389
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 2 cut(s) 427, 437
LguI GCTCTTC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 276
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 3 cut(s) 148, 246, 400
MaeI CTAG 2 cut(s) 159, 579
MaeIII GTNAC 3 cut(s) 371, 493, 554
MalI GATC 2 cut(s) 429, 439
MboI GATC 2 cut(s) 427, 437
MboII GAAGA 1 cut(s) 120
MluCI AATT 2 cut(s) 242, 318
MlyI GAGTC 1 cut(s) 163
MnlI CCTC 3 cut(s) 112, 209, 560
MseI TTAA 2 cut(s) 408, 434
MslI CAYNNNNRTG 1 cut(s) 282
MspR9I CCNGG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 1 cut(s) 42
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 2 cut(s) 173, 179
NdeI CATATG 2 cut(s) 283, 538
NdeII GATC 2 cut(s) 427, 437
NlaIII CATG 2 cut(s) 512, 526
NlaIV GGNNCC 2 cut(s) 46, 100
NmuCI GTSAC 1 cut(s) 371
PagI TCATGA 1 cut(s) 508
PciSI GCTCTTC 1 cut(s) 103
PctI GAATGC 1 cut(s) 277
PfeI GAWTC 3 cut(s) 73, 445, 466
PflFI GACNNNGTC 1 cut(s) 131
PkrI GCNGC 2 cut(s) 175, 423
PleI GAGTC 1 cut(s) 162
PpsI GAGTC 1 cut(s) 162
PpuMI RGGWCCY 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 44
Psp6I CCWGG 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 46, 100
PspPI GGNCC 4 cut(s) 38, 44, 311, 547
PspPPI RGGWCCY 1 cut(s) 44
PsyI GACNNNGTC 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 282
SapI GCTCTTC 1 cut(s) 103
SaqAI TTAA 2 cut(s) 408, 434
SatI GCNGC 2 cut(s) 174, 422
Sau3AI GATC 2 cut(s) 427, 437
Sau96I GGNCC 4 cut(s) 38, 44, 311, 547
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 42
SetI ASST 9 cut(s) 46, 60, 100, 273, 316, 414, 426, 561, 571
SfaNI GCATC 3 cut(s) 148, 246, 400
SinI GGWCC 3 cut(s) 38, 44, 311
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 2 cut(s) 242, 318
SsiI CCGC 1 cut(s) 173
SspMI CTAG 2 cut(s) 159, 579
StyD4I CCNGG 1 cut(s) 40
TaaI ACNGT 1 cut(s) 253
TasI AATT 2 cut(s) 242, 318
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 3 cut(s) 73, 445, 466
Tru1I TTAA 2 cut(s) 408, 434
Tru9I TTAA 2 cut(s) 408, 434
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 4 cut(s) 20, 270, 511, 520
TspRI CASTG 1 cut(s) 447
Tth111I GACNNNGTC 1 cut(s) 131
VpaK11BI GGWCC 3 cut(s) 38, 44, 311
XapI RAATTY 1 cut(s) 242
XspI CTAG 2 cut(s) 159, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.