MD05G1165500.v1.1

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008764
Physical Location & Seq
Reverse (-)
29397189 .. 29398884
1696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1165500.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGATAGTCGTCCCTCTGAAACTCAAGGCACAGCCCAGGCGAACAATCCTTATGTCCATTATTCTCCTGCTCCGGCCACATCGAATTCTTCTACCCAGCATCCGATGGCAAATATGGTTGAGGCATTGTGCCAGTGCGGTAAAAGGGTTGAAGTTGCCACCAAAAGAGCAGAGGCCAAGGCGGACAATGTCTGGAACCACCTGAGAATGTGCCCGAGTTTCACAGAAGCAATGATGGCAAGGCTAAGTACCGGAACAAAGGTGCTTACTGCCGGAGGAGAAGAAAAAGTGTTTCAGCAGACATTTGAAAGAGTGACAGGAGAAAAGCTCTTACATTCATATGCTTGCCATCTATCAACTTCTCATGGACCTGTGATTGGGATGATGTACATCTCCGATAAACGAATAGCCTTCTGCAGCGATTTCTCTTACTATTTGTGCCCAGGAAATCCAACTCTCATGCATTACAAGGTGGTGGTGCCACTTGATCAGTTAAGAACAGTAAATCCTTCAGCAAACAGATGGAACCCTTCAGAAAAATATATACAGATTGTCACAAGGGATGGTCATGAATTCTGGTTCATGGGGTTCATATCCTATGACAAAGCCCTCAGGAATCTAAGCCAAGCTCCACGGCTATCTCAGAATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

218

Amino Acids

24.39

Weight (kDa)

8.72

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 97 - 204 4.9e-16 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 478
AciI CCGC 2 cut(s) 138, 182
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 2 cut(s) 85, 572
AcuI CTGAAG 2 cut(s) 495, 516
AfaI GTAC 2 cut(s) 250, 389
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 152, 308
AjnI CCWGG 2 cut(s) 36, 442
AloI GAACNNNNNNTCC 2 cut(s) 490, 522
AluBI AGCT 2 cut(s) 328, 629
AluI AGCT 2 cut(s) 328, 629
Ama87I CYCGRG 1 cut(s) 214
AoxI GGCC 2 cut(s) 75, 174
ApeKI GCWGC 1 cut(s) 417
ApoI RAATTY 2 cut(s) 85, 572
AspS9I GGNCC 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 214
AvaII GGWCC 1 cut(s) 368
AxyI CCTNAGG 1 cut(s) 611
BaeGI GKGCMC 2 cut(s) 215, 443
BanI GGYRCC 1 cut(s) 478
BbvI GCAGC 1 cut(s) 429
BccI CCATC 5 cut(s) 100, 229, 357, 516, 557
BceAI ACGGC 1 cut(s) 650
BciT130I CCWGG 2 cut(s) 38, 444
BclI TGATCA 1 cut(s) 487
BfmI CTRYAG 1 cut(s) 415
BisI GCNGC 1 cut(s) 418
BlsI GCNGC 1 cut(s) 419
Bme1390I CCNGG 2 cut(s) 38, 444
Bme18I GGWCC 1 cut(s) 368
BmeT110I CYCGRG 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 3 cut(s) 197, 480, 527
BmrFI CCNGG 2 cut(s) 38, 444
BmsI GCATC 1 cut(s) 109
BplI GAGNNNNNCTC 2 cut(s) 312, 344
BpuEI CTTGAG 1 cut(s) 9
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 4 cut(s) 36, 177, 442, 632
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 133
Bse21I CCTNAGG 1 cut(s) 611
Bse3DI GCAATG 1 cut(s) 237
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 2 cut(s) 38, 444
BseDI CCNNGG 4 cut(s) 36, 177, 442, 632
BseGI GGATG 3 cut(s) 100, 387, 568
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 2 cut(s) 194, 625
BseNI ACTGG 1 cut(s) 133
BseRI GAGGAG 1 cut(s) 291
BseSI GKGCMC 2 cut(s) 215, 443
BseXI GCAGC 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 96
BshFI GGCC 2 cut(s) 77, 176
BshNI GGYRCC 1 cut(s) 478
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 3 cut(s) 74, 252, 273
BslI CCNNNNNNNGG 1 cut(s) 377
BsnI GGCC 2 cut(s) 77, 176
BsoBI CYCGRG 1 cut(s) 214
Bsp1286I GDGCHC 2 cut(s) 215, 443
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 1 cut(s) 487
BspACI CCGC 2 cut(s) 138, 182
BspANI GGCC 2 cut(s) 77, 176
BspCNI CTCAG 2 cut(s) 195, 624
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 3 cut(s) 197, 480, 527
BspMAI CTGCAG 1 cut(s) 419
BspT107I GGYRCC 1 cut(s) 478
BsrDI GCAATG 1 cut(s) 237
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 4 cut(s) 36, 177, 442, 632
BssMI GATC 1 cut(s) 487
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 2 cut(s) 38, 444
Bst4CI ACNGT 1 cut(s) 502
BstAUI TGTACA 1 cut(s) 387
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 5 cut(s) 203, 245, 611, 620, 642
BstDSI CCRYGG 1 cut(s) 632
BstF5I GGATG 3 cut(s) 100, 387, 568
BstKTI GATC 1 cut(s) 490
BstMBI GATC 1 cut(s) 487
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstNI CCWGG 2 cut(s) 38, 444
BstSCI CCNGG 2 cut(s) 36, 442
BstSFI CTRYAG 1 cut(s) 415
BstSLI GKGCMC 2 cut(s) 215, 443
BstV1I GCAGC 1 cut(s) 429
Bsu36I CCTNAGG 1 cut(s) 611
BsuRI GGCC 2 cut(s) 77, 176
BtgI CCRYGG 1 cut(s) 632
BtsCI GGATG 3 cut(s) 100, 387, 568
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 346
CciI TCATGA 1 cut(s) 568
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 249, 388
CviAII CATG 4 cut(s) 365, 460, 569, 583
CviQI GTAC 2 cut(s) 249, 388
DdeI CTNAG 5 cut(s) 203, 245, 611, 620, 642
DpnI GATC 1 cut(s) 489
DpnII GATC 1 cut(s) 487
EaeI YGGCCR 1 cut(s) 75
EciI GGCGGA 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 368
Eco57I CTGAAG 2 cut(s) 495, 516
Eco81I CCTNAGG 1 cut(s) 611
Eco88I CYCGRG 1 cut(s) 214
EcoRI GAATTC 2 cut(s) 85, 572
EcoRII CCWGG 2 cut(s) 36, 442
EcoT14I CCWWGG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 177
FaeI CATG 4 cut(s) 368, 463, 572, 586
FatI CATG 4 cut(s) 364, 459, 568, 582
FauNDI CATATG 1 cut(s) 340
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 1 cut(s) 418
FokI GGATG 3 cut(s) 87, 394, 575
Fsp4HI GCNGC 1 cut(s) 418
GluI GCNGC 1 cut(s) 418
GsaI CCCAGC 1 cut(s) 100
HaeIII GGCC 2 cut(s) 77, 176
HapII CCGG 3 cut(s) 74, 252, 273
Hin1II CATG 4 cut(s) 368, 463, 572, 586
HinfI GANTC 1 cut(s) 616
HpaII CCGG 3 cut(s) 74, 252, 273
Hpy188I TCNGA 5 cut(s) 19, 105, 397, 535, 645
Hpy188III TCNNGA 3 cut(s) 193, 569, 613
HpyAV CCTTC 3 cut(s) 421, 519, 540
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4V TGCA 2 cut(s) 417, 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 5 cut(s) 203, 245, 611, 620, 642
Hsp92II CATG 4 cut(s) 368, 463, 572, 586
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 1 cut(s) 487
LmnI GCTCC 2 cut(s) 76, 634
Lsp1109I GCAGC 1 cut(s) 429
LweI GCATC 1 cut(s) 109
MaeIII GTNAC 2 cut(s) 313, 553
MalI GATC 1 cut(s) 489
MboI GATC 1 cut(s) 487
MboII GAAGA 2 cut(s) 81, 293
MhlI GDGCHC 2 cut(s) 215, 443
MluCI AATT 2 cut(s) 85, 572
MmeI TCCRAC 1 cut(s) 476
MnlI CCTC 5 cut(s) 25, 115, 166, 269, 620
Mph1103I ATGCAT 1 cut(s) 465
MseI TTAA 1 cut(s) 494
MslI CAYNNNNRTG 1 cut(s) 339
MspI CCGG 3 cut(s) 74, 252, 273
MspR9I CCNGG 2 cut(s) 38, 444
MvaI CCWGG 2 cut(s) 38, 444
MwoI GCNNNNNNNGC 1 cut(s) 236
NdeI CATATG 1 cut(s) 340
NdeII GATC 1 cut(s) 487
NlaIII CATG 4 cut(s) 368, 463, 572, 586
NlaIV GGNNCC 3 cut(s) 197, 480, 527
NmuCI GTSAC 2 cut(s) 313, 553
NsiI ATGCAT 1 cut(s) 465
PagI TCATGA 1 cut(s) 568
PfeI GAWTC 1 cut(s) 616
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 36, 442
PspFI CCCAGC 1 cut(s) 96
PspGI CCWGG 2 cut(s) 36, 442
PspN4I GGNNCC 3 cut(s) 197, 480, 527
PspPI GGNCC 1 cut(s) 368
PstI CTGCAG 1 cut(s) 419
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 2 cut(s) 250, 389
RsaNI GTAC 2 cut(s) 249, 388
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 494
SatI GCNGC 1 cut(s) 418
Sau3AI GATC 1 cut(s) 487
Sau96I GGNCC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 38, 444
SduI GDGCHC 2 cut(s) 215, 443
SetI ASST 6 cut(s) 204, 264, 330, 373, 474, 631
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 415
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 24
SmoI CTYRAG 1 cut(s) 24
Sse9I AATT 2 cut(s) 85, 572
SsiI CCGC 2 cut(s) 138, 182
StyD4I CCNGG 2 cut(s) 36, 442
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 502
TaqI TCGA 1 cut(s) 83
TasI AATT 2 cut(s) 85, 572
TatI WGTACW 1 cut(s) 387
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TscAI CASTG 1 cut(s) 140
TseFI GTSAC 2 cut(s) 313, 553
TseI GCWGC 1 cut(s) 417
Tsp45I GTSAC 2 cut(s) 313, 553
TspDTI ATGAA 4 cut(s) 327, 571, 580, 585
TspRI CASTG 1 cut(s) 140
Tth111I GACNNNGTC 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 2 cut(s) 85, 572
Zsp2I ATGCAT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.