MD10G1154900.v1.1

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008764
Physical Location & Seq
Reverse (-)
24252481 .. 24253477
997 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1154900.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGTGGACAATGTCTGGGACCACTCGAGAATGTGTCCAAGTTTCACAGAAGCAGTGAAAGCAAGGCTAAATACCGGCACAAAGGTGCTTACTGCCGGAGGACAAGAAAAAGCATTCCAGCAGACATTTGAAAGAATTACAGGAGAGAAGCTCTTACATTCATATGTTTGCTATCTATCAACTTCTCAAGGACCTGTGATTGGGATGCTGTACATCTCTAATGTACGAATAGCCTTCTGCAGTGATTTTTCACACTATTTGTCCCCAGGACATCCAACTTCCATGCATTACAAGGTGGTGGTGCAGCTTGATCAGTTAAGAACAGTAAATCCTTCAGCAAACATATGGAACCCTTCAGAAAAGTATATACACTTTGTCACAAGAGATGGTCATGAATTCTGGTTCATGTGGTTCATATCCTATGACAAGGCCCTCGGTAATCTTAGCCGAGCTATACAGCTATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

156

Amino Acids

17.74

Weight (kDa)

8.46

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 38 - 144 9e-13 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 395
AcuI CTGAAG 2 cut(s) 318, 339
AfaI GTAC 2 cut(s) 212, 225
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 1 cut(s) 131
AjnI CCWGG 1 cut(s) 265
AleI CACNNNNGTG 1 cut(s) 83
AloI GAACNNNNNNTCC 2 cut(s) 313, 345
AluBI AGCT 4 cut(s) 151, 307, 452, 460
AluI AGCT 4 cut(s) 151, 307, 452, 460
Ama87I CYCGRG 1 cut(s) 25
AoxI GGCC 1 cut(s) 429
ApeKI GCWGC 1 cut(s) 304
ApoI RAATTY 1 cut(s) 395
AspS9I GGNCC 3 cut(s) 19, 191, 430
AvaI CYCGRG 1 cut(s) 25
AvaII GGWCC 2 cut(s) 19, 191
BbvI GCAGC 1 cut(s) 316
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 267
BclI TGATCA 1 cut(s) 310
BfmI CTRYAG 1 cut(s) 238
BisI GCNGC 1 cut(s) 305
BlsI GCNGC 1 cut(s) 306
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 2 cut(s) 19, 191
BmeT110I CYCGRG 1 cut(s) 25
BmgT120I GGNCC 3 cut(s) 19, 191, 430
BmiI GGNNCC 2 cut(s) 20, 350
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpuEI CTTGAG 1 cut(s) 171
BsaJI CCNNGG 2 cut(s) 265, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 74
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 265, 433
BseGI GGATG 2 cut(s) 210, 271
BseLI CCNNNNNNNGG 1 cut(s) 200
BseXI GCAGC 1 cut(s) 316
BsgI GTGCAG 1 cut(s) 323
BshFI GGCC 1 cut(s) 431
BsiHKCI CYCGRG 1 cut(s) 25
BsiSI CCGG 2 cut(s) 75, 96
BslFI GGGAC 2 cut(s) 32, 247
BslI CCNNNNNNNGG 1 cut(s) 200
BsmFI GGGAC 2 cut(s) 32, 247
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 1 cut(s) 431
BsoBI CYCGRG 1 cut(s) 25
Bsp1407I TGTACA 1 cut(s) 210
Bsp143I GATC 1 cut(s) 310
BspANI GGCC 1 cut(s) 431
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 2 cut(s) 20, 350
BspMAI CTGCAG 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 74
BsrGI TGTACA 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 2 cut(s) 265, 433
BssMI GATC 1 cut(s) 310
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 325
BstAUI TGTACA 1 cut(s) 210
BstDEI CTNAG 2 cut(s) 443, 465
BstF5I GGATG 2 cut(s) 210, 271
BstKTI GATC 1 cut(s) 313
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 238
BstV1I GCAGC 1 cut(s) 316
BsuRI GGCC 1 cut(s) 431
BtsCI GGATG 2 cut(s) 210, 271
BtsI GCAGTG 2 cut(s) 60, 247
BtsIMutI CAGTG 2 cut(s) 60, 247
CciI TCATGA 1 cut(s) 391
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 3 cut(s) 19, 191, 430
Csp6I GTAC 2 cut(s) 211, 224
CviAII CATG 3 cut(s) 283, 392, 406
CviJI RGCY 8 cut(s) 67, 151, 233, 307, 431, 447, 452, 460
CviKI_1 RGCY 8 cut(s) 67, 151, 233, 307, 431, 447, 452, 460
CviQI GTAC 2 cut(s) 211, 224
DdeI CTNAG 2 cut(s) 443, 465
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eco47I GGWCC 2 cut(s) 19, 191
Eco57I CTGAAG 2 cut(s) 318, 339
Eco88I CYCGRG 1 cut(s) 25
EcoO109I RGGNCCY 2 cut(s) 191, 430
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 265
EcoT22I ATGCAT 1 cut(s) 288
FaeI CATG 3 cut(s) 286, 395, 409
FaqI GGGAC 2 cut(s) 32, 247
FatI CATG 3 cut(s) 282, 391, 405
FauNDI CATATG 2 cut(s) 163, 344
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 305
FokI GGATG 2 cut(s) 217, 258
Fsp4HI GCNGC 1 cut(s) 305
GluI GCNGC 1 cut(s) 305
HaeIII GGCC 1 cut(s) 431
HapII CCGG 2 cut(s) 75, 96
Hin1II CATG 3 cut(s) 286, 395, 409
HpaII CCGG 2 cut(s) 75, 96
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 1 cut(s) 358
Hpy188III TCNNGA 2 cut(s) 27, 392
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 3 cut(s) 244, 342, 363
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4V TGCA 3 cut(s) 240, 286, 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 2 cut(s) 443, 465
Hsp92II CATG 3 cut(s) 286, 395, 409
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 1 cut(s) 310
LpnPI CCDG 8 cut(s) 88, 109, 126, 131, 207, 252, 279, 385
Lsp1109I GCAGC 1 cut(s) 316
LweI GCATC 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 376
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MluCI AATT 2 cut(s) 135, 395
MmeI TCCRAC 1 cut(s) 299
MnlI CCTC 2 cut(s) 92, 443
Mph1103I ATGCAT 1 cut(s) 288
MseI TTAA 1 cut(s) 317
MslI CAYNNNNRTG 2 cut(s) 83, 162
MspI CCGG 2 cut(s) 75, 96
MspR9I CCNGG 1 cut(s) 267
Mva1269I GAATGC 1 cut(s) 113
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 59
NdeI CATATG 2 cut(s) 163, 344
NdeII GATC 1 cut(s) 310
NlaIII CATG 3 cut(s) 286, 395, 409
NlaIV GGNNCC 2 cut(s) 20, 350
NmuCI GTSAC 1 cut(s) 376
NsiI ATGCAT 1 cut(s) 288
OliI CACNNNNGTG 1 cut(s) 83
PaeR7I CTCGAG 1 cut(s) 25
PagI TCATGA 1 cut(s) 391
PctI GAATGC 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 1 cut(s) 306
PpuMI RGGWCCY 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 2 cut(s) 20, 350
PspPI GGNCC 3 cut(s) 19, 191, 430
PspPPI RGGWCCY 1 cut(s) 191
PstI CTGCAG 1 cut(s) 242
PsyI GACNNNGTC 1 cut(s) 11
RsaI GTAC 2 cut(s) 212, 225
RsaNI GTAC 2 cut(s) 211, 224
RseI CAYNNNNRTG 2 cut(s) 83, 162
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 1 cut(s) 305
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 3 cut(s) 19, 191, 430
ScrFI CCNGG 1 cut(s) 267
SetI ASST 7 cut(s) 87, 153, 196, 297, 309, 454, 462
SfaNI GCATC 1 cut(s) 195
SfcI CTRYAG 1 cut(s) 238
Sfr274I CTCGAG 1 cut(s) 25
SinI GGWCC 2 cut(s) 19, 191
SlaI CTCGAG 1 cut(s) 25
SmiMI CAYNNNNRTG 2 cut(s) 83, 162
SmlI CTYRAG 2 cut(s) 25, 186
SmoI CTYRAG 2 cut(s) 25, 186
Sse9I AATT 2 cut(s) 135, 395
StyD4I CCNGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 325
TaqI TCGA 1 cut(s) 26
TasI AATT 2 cut(s) 135, 395
TatI WGTACW 1 cut(s) 210
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 2 cut(s) 60, 247
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 4 cut(s) 150, 394, 403, 408
TspRI CASTG 2 cut(s) 60, 247
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 2 cut(s) 19, 191
XapI RAATTY 1 cut(s) 395
XhoI CTCGAG 1 cut(s) 25
Zsp2I ATGCAT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.