Rh6CG269300

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008764
Physical Location & Seq
Reverse (-)
44367999 .. 44370170
2172 bp
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UTR
Exon/CDS
Intron
Rh6CG269300.1

Sequence Viewer

Length: 705 bp
ATGAACATGATGGTGAATGGCAGTCCTCCTCCACCTCCTCCTCCTCAAATTGAAGGCAATGCCTACGCCGACTTCAACCACAACAATCCTTATGTTCAATATTCCCCAGTTCCCACGTCGAATTCACCTACCAAGCGTCCCATAGATAAAGTGCTTGACACATTGAATCATTGTGGTAAAAGGGTTGAAGGTGCCTCCAGAAGAGCAGACACCATTACAGACAATGTCTGGCATCACTTGAGAATGAGTCCTAGTCTTGCAGACGCGGCAATGGCAAGGCTTACTCACGGGACAAAGGCTCTTACAAGAGGGGATGATAAAGTATTCCAGCAAAAATTTGAGACTGTGATGCTACAAGAGAAGCTCCTGCATTCATATGCTTGCTATCTCTCAACTTCTACTGGACCTGTAATTGGAGTGCTGTATATTTCCAATAAAAGAATAGCCTTTTGTAGTGATTTCTGTCACTATATTTCCCCAGCGCATCCGAACACATATTTTAAGGTGGTGGTGCAGCTTGATCAGTTAAGAGCAGTGAATCCTTCTGCAAACAGATGGAATCCTTCAGAGAAATACATACAGATAGTAACAAGAGACGGTCATGAGTTCTGGTTCATGGGGTTCATATCTTATGACAAGGCCCTCAGTAACCTAACCCAAGCTCTCAACCCTAGTTCTGGTGGAACTCATCTCAACCCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAM

Protein Analysis

234

Amino Acids

26.22

Weight (kDa)

9.0

Isoelectric Point (pI)

52.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 108 - 222 9.2e-17 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 191
AccII CGCG 1 cut(s) 266
AciI CCGC 1 cut(s) 266
AcsI RAATTY 2 cut(s) 121, 335
AcuI CTGAAG 1 cut(s) 549
AfiI CCNNNNNNNGG 2 cut(s) 413, 677
AgsI TTSAA 5 cut(s) 53, 76, 98, 166, 188
AjiI CACGTC 1 cut(s) 117
AluBI AGCT 3 cut(s) 364, 517, 662
AluI AGCT 3 cut(s) 364, 517, 662
Alw26I GTCTC 2 cut(s) 335, 588
AoxI GGCC 1 cut(s) 639
ApeKI GCWGC 1 cut(s) 514
ApoI RAATTY 2 cut(s) 121, 335
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 2 cut(s) 404, 640
AsuHPI GGTGA 2 cut(s) 25, 117
AvaII GGWCC 1 cut(s) 404
BanI GGYRCC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 526
BccI CCATC 2 cut(s) 4, 549
BclI TGATCA 1 cut(s) 520
BcoDI GTCTC 2 cut(s) 335, 588
BfaI CTAG 2 cut(s) 252, 672
BisI GCNGC 2 cut(s) 267, 515
BlsI GCNGC 2 cut(s) 268, 516
Bme18I GGWCC 1 cut(s) 404
BmgBI CACGTC 1 cut(s) 117
BmgT120I GGNCC 2 cut(s) 404, 640
BmiI GGNNCC 1 cut(s) 193
BmrI ACTGGG 1 cut(s) 101
BmsI GCATC 3 cut(s) 241, 339, 493
BmuI ACTGGG 1 cut(s) 101
BpmI CTGGAG 1 cut(s) 181
BpuEI CTTGAG 1 cut(s) 259
Bsc4I CCNNNNNNNGG 2 cut(s) 413, 677
Bse1I ACTGG 2 cut(s) 107, 406
Bse3DI GCAATG 2 cut(s) 64, 276
BseGI GGATG 2 cut(s) 319, 484
BseLI CCNNNNNNNGG 2 cut(s) 413, 677
BseMI GCAATG 2 cut(s) 64, 276
BseMII CTCAG 1 cut(s) 658
BseNI ACTGG 2 cut(s) 107, 406
BseRI GAGGAG 4 cut(s) 18, 27, 30, 33
BseXI GCAGC 1 cut(s) 526
BseYI CCCAGC 1 cut(s) 478
BsgI GTGCAG 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 1 cut(s) 641
BshNI GGYRCC 1 cut(s) 191
BslFI GGGAC 2 cut(s) 123, 304
BslI CCNNNNNNNGG 2 cut(s) 413, 677
BsmAI GTCTC 2 cut(s) 335, 588
BsmBI CGTCTC 1 cut(s) 588
BsmFI GGGAC 2 cut(s) 123, 304
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 1 cut(s) 641
Bsp143I GATC 1 cut(s) 520
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 641
BspCNI CTCAG 1 cut(s) 657
BspFNI CGCG 1 cut(s) 266
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 1 cut(s) 193
BspQI GCTCTTC 1 cut(s) 196
BspT107I GGYRCC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 64, 276
BsrI ACTGG 2 cut(s) 107, 406
BssMI GATC 1 cut(s) 520
Bst4CI ACNGT 2 cut(s) 346, 599
Bst6I CTCTTC 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 644
BstF5I GGATG 2 cut(s) 319, 484
BstFNI CGCG 1 cut(s) 266
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 1 cut(s) 523
BstMAI GTCTC 2 cut(s) 335, 588
BstMBI GATC 1 cut(s) 520
BstMWI GCNNNNNNNGC 2 cut(s) 266, 272
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 526
BsuRI GGCC 1 cut(s) 641
BtrI CACGTC 1 cut(s) 117
BtsCI GGATG 2 cut(s) 319, 484
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 1 cut(s) 540
Cac8I GCNNGC 1 cut(s) 382
CciI TCATGA 1 cut(s) 601
CfoI GCGC 1 cut(s) 484
Cfr13I GGNCC 2 cut(s) 404, 640
CseI GACGC 2 cut(s) 125, 272
CviAII CATG 3 cut(s) 7, 602, 616
CviJI RGCY 7 cut(s) 280, 299, 364, 446, 517, 641, 662
CviKI_1 RGCY 7 cut(s) 280, 299, 364, 446, 517, 641, 662
DdeI CTNAG 1 cut(s) 644
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 404
Eco57I CTGAAG 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 640
EcoRI GAATTC 1 cut(s) 121
Esp3I CGTCTC 1 cut(s) 588
FaeI CATG 3 cut(s) 10, 605, 619
FaqI GGGAC 2 cut(s) 123, 304
FatI CATG 3 cut(s) 6, 601, 615
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 1 cut(s) 520
Fnu4HI GCNGC 2 cut(s) 267, 515
FokI GGATG 2 cut(s) 326, 471
Fsp4HI GCNGC 2 cut(s) 267, 515
FspBI CTAG 2 cut(s) 252, 672
GlaI GCGC 1 cut(s) 483
GluI GCNGC 2 cut(s) 267, 515
GsaI CCCAGC 1 cut(s) 482
GsuI CTGGAG 1 cut(s) 181
HaeIII GGCC 1 cut(s) 641
HgaI GACGC 2 cut(s) 125, 272
HhaI GCGC 1 cut(s) 484
Hin1II CATG 3 cut(s) 10, 605, 619
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HinfI GANTC 4 cut(s) 166, 247, 538, 559
HphI GGTGA 2 cut(s) 25, 117
Hpy188I TCNGA 2 cut(s) 489, 568
Hpy188III TCNNGA 2 cut(s) 198, 602
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 4 cut(s) 47, 182, 552, 573
HpyCH4III ACNGT 2 cut(s) 346, 599
HpyCH4IV ACGT 1 cut(s) 116
HpyCH4V TGCA 4 cut(s) 260, 370, 514, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 266, 272
HpyF3I CTNAG 1 cut(s) 644
HpySE526I ACGT 1 cut(s) 116
Hsp92II CATG 3 cut(s) 10, 605, 619
HspAI GCGC 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 1 cut(s) 520
LguI GCTCTTC 1 cut(s) 196
LmnI GCTCC 1 cut(s) 369
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 3 cut(s) 241, 339, 493
MaeI CTAG 2 cut(s) 252, 672
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 3 cut(s) 464, 586, 647
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 1 cut(s) 213
MluCI AATT 4 cut(s) 48, 121, 335, 411
MlyI GAGTC 1 cut(s) 256
MnlI CCTC 9 cut(s) 36, 39, 45, 48, 51, 54, 205, 302, 653
MseI TTAA 2 cut(s) 501, 527
MslI CAYNNNNRTG 2 cut(s) 11, 375
Mva1269I GAATGC 1 cut(s) 370
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 266, 272
NdeI CATATG 1 cut(s) 376
NdeII GATC 1 cut(s) 520
NlaIII CATG 3 cut(s) 10, 605, 619
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 464
PagI TCATGA 1 cut(s) 601
PciSI GCTCTTC 1 cut(s) 196
PctI GAATGC 1 cut(s) 370
PfeI GAWTC 3 cut(s) 166, 538, 559
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 2 cut(s) 268, 516
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PspFI CCCAGC 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 2 cut(s) 404, 640
PsyI GACNNNGTC 1 cut(s) 224
RseI CAYNNNNRTG 2 cut(s) 11, 375
SapI GCTCTTC 1 cut(s) 196
SaqAI TTAA 2 cut(s) 501, 527
SatI GCNGC 2 cut(s) 267, 515
Sau3AI GATC 1 cut(s) 520
Sau96I GGNCC 2 cut(s) 404, 640
SchI GAGTC 1 cut(s) 256
SfaNI GCATC 3 cut(s) 241, 339, 493
SinI GGWCC 1 cut(s) 404
SmiMI CAYNNNNRTG 2 cut(s) 11, 375
SmlI CTYRAG 1 cut(s) 238
SmoI CTYRAG 1 cut(s) 238
Sse9I AATT 4 cut(s) 48, 121, 335, 411
SsiI CCGC 1 cut(s) 266
SspI AATATT 1 cut(s) 101
SspMI CTAG 2 cut(s) 252, 672
TaaI ACNGT 2 cut(s) 346, 599
TaiI ACGT 1 cut(s) 119
TaqI TCGA 1 cut(s) 119
TasI AATT 4 cut(s) 48, 121, 335, 411
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 3 cut(s) 166, 538, 559
Tru1I TTAA 2 cut(s) 501, 527
Tru9I TTAA 2 cut(s) 501, 527
TscAI CASTG 1 cut(s) 540
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 1 cut(s) 514
Tsp45I GTSAC 1 cut(s) 464
TspDTI ATGAA 4 cut(s) 17, 363, 604, 613
TspRI CASTG 1 cut(s) 540
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 2 cut(s) 121, 335
XspI CTAG 2 cut(s) 252, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.