AT4G01995

Beta-carotene isomerase D27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002743
Physical Location & Seq
Forward (+)
872923 .. 874772
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G01995.1

Sequence Viewer

Length: 777 bp
ATGGCTGTTCTTATCCAGGTTCTTCTTCCTCCTCCGACGACTATCGTCTTCTCCAACTCATTTTCTACAAAGTTAATACTCACGCGTCGTCTTCGTTGCCGTATCTCTAATTCGTCAGAGGTTAAATCAGATGAAGGAGCTCCGAAGTTGGAGTATAAGCCAGGACCTCTCGACGATTTCTTCATGCAATCGTTCCGGAACAAATTGGTCGAGGAAGTCGGGTCGGATTCAGAGAAACCCGGGTACGTTGGACTTATTGAGTTGGTTAAGCTTCTCTTGTTGAAAGGTCGTACTAGGTCGGAGACAAGTGATGCTGCGGTTCGAATACTGAAATCGTTGTTTCCTCCGCTAATTTTAGAGCTTTACAAGCTTCTCATTGCTCCTATAGCACAAGGAAAATTAGCTGCTTTGATGGTTGCAAGAGTTACTGTACTTACATGTCAATGGCTCATGGGACCGAGCAAAGTAAACATCATCGATCTTCCTAATGGAGAATCTTGGGATAGCGGGGTTTTCGTGGAGAAATGTCAGTATCTAGAAGAAAGCAAGTGTGTAGGAGTATGCATTAATACATGTAAACTACCAACTCAGACATTCTTTAAAGACTACATGGGAGTTCCTTTGGTGATGGAACCAAACTTCAAAGATTATAGTTGCCAGTTCAAATTCGGAGTAGCTCCACCGGAGGATGATGGCAATGTTAATGAACCATGTTTTGAGACATGCTCAATCGCTGGCCGCCGGAAATTAAAATCCGGGGAGTGTCCTTTAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

258

Amino Acids

28.64

Weight (kDa)

7.97

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 149 - 229 1.5e-34 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 85
AccIII TCCGGA 1 cut(s) 195
AciI CCGC 4 cut(s) 317, 347, 507, 739
AcoI YGGCCR 1 cut(s) 736
AcsI RAATTY 1 cut(s) 665
AfaI GTAC 3 cut(s) 245, 292, 432
AflIII ACRYGT 3 cut(s) 83, 437, 572
AgsI TTSAA 3 cut(s) 283, 643, 664
AjnI CCWGG 2 cut(s) 15, 160
AluBI AGCT 6 cut(s) 140, 271, 361, 370, 404, 677
AluI AGCT 6 cut(s) 140, 271, 361, 370, 404, 677
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 2 cut(s) 296, 713
Ama87I CYCGRG 1 cut(s) 239
Aor13HI TCCGGA 1 cut(s) 195
AoxI GGCC 1 cut(s) 736
ApeKI GCWGC 2 cut(s) 314, 404
ApoI RAATTY 1 cut(s) 665
AseI ATTAAT 1 cut(s) 567
AspS9I GGNCC 2 cut(s) 164, 455
AsuC2I CCSGG 3 cut(s) 240, 241, 757
AsuHPI GGTGA 1 cut(s) 637
AsuII TTCGAA 1 cut(s) 322
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 2 cut(s) 164, 455
BanII GRGCYC 1 cut(s) 142
BbsI GAAGAC 2 cut(s) 40, 83
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 2 cut(s) 301, 391
BccI CCATC 3 cut(s) 406, 622, 686
BceAI ACGGC 1 cut(s) 84
BciT130I CCWGG 2 cut(s) 17, 162
BcnI CCSGG 3 cut(s) 240, 241, 757
BcoDI GTCTC 2 cut(s) 296, 713
BfaI CTAG 2 cut(s) 294, 536
BfmI CTRYAG 1 cut(s) 384
BisI GCNGC 3 cut(s) 315, 405, 739
BlsI GCNGC 3 cut(s) 316, 406, 740
Bme1390I CCNGG 5 cut(s) 17, 162, 240, 241, 757
Bme18I GGWCC 2 cut(s) 164, 455
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 164, 455
BmiI GGNNCC 2 cut(s) 456, 633
BmrFI CCNGG 5 cut(s) 17, 162, 240, 241, 757
BmsI GCATC 1 cut(s) 301
BoxI GACNNNNGTC 1 cut(s) 44
BpiI GAAGAC 2 cut(s) 40, 83
BplI GAGNNNNNCTC 2 cut(s) 710, 742
Bpu14I TTCGAA 1 cut(s) 322
BpuMI CCSGG 3 cut(s) 240, 241, 757
Bsa29I ATCGAT 1 cut(s) 477
BsaJI CCNNGG 2 cut(s) 239, 756
BsaWI WCCGGW 2 cut(s) 195, 682
Bse1I ACTGG 1 cut(s) 658
Bse3DI GCAATG 2 cut(s) 375, 703
BseAI TCCGGA 1 cut(s) 195
BseBI CCWGG 2 cut(s) 17, 162
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 239, 756
BseGI GGATG 1 cut(s) 694
BseMI GCAATG 2 cut(s) 375, 703
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 1 cut(s) 658
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 2 cut(s) 301, 391
Bsh1236I CGCG 1 cut(s) 85
BshFI GGCC 1 cut(s) 738
BshVI ATCGAT 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 142
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 5 cut(s) 196, 240, 683, 742, 756
BslFI GGGAC 1 cut(s) 468
BsmAI GTCTC 2 cut(s) 296, 713
BsmFI GGGAC 1 cut(s) 468
BsnI GGCC 1 cut(s) 738
BsoBI CYCGRG 1 cut(s) 239
Bsp119I TTCGAA 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 142
Bsp13I TCCGGA 1 cut(s) 195
Bsp143I GATC 1 cut(s) 478
BspACI CCGC 4 cut(s) 317, 347, 507, 739
BspANI GGCC 1 cut(s) 738
BspCNI CTCAG 1 cut(s) 601
BspDI ATCGAT 1 cut(s) 477
BspEI TCCGGA 1 cut(s) 195
BspFNI CGCG 1 cut(s) 85
BspLI GGNNCC 2 cut(s) 456, 633
BspT104I TTCGAA 1 cut(s) 322
BsrDI GCAATG 2 cut(s) 375, 703
BsrI ACTGG 1 cut(s) 658
BssECI CCNNGG 2 cut(s) 239, 756
BssMI GATC 1 cut(s) 478
Bst2UI CCWGG 2 cut(s) 17, 162
Bst4CI ACNGT 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 322
BstC8I GCNNGC 1 cut(s) 736
BstDEI CTNAG 1 cut(s) 588
BstF5I GGATG 1 cut(s) 694
BstFNI CGCG 1 cut(s) 85
BstKTI GATC 1 cut(s) 481
BstMAI GTCTC 2 cut(s) 296, 713
BstMBI GATC 1 cut(s) 478
BstMWI GCNNNNNNNGC 2 cut(s) 367, 386
BstNI CCWGG 2 cut(s) 17, 162
BstNSI RCATGY 3 cut(s) 441, 576, 726
BstPAI GACNNNNGTC 1 cut(s) 44
BstSCI CCNGG 5 cut(s) 15, 160, 238, 239, 755
BstSFI CTRYAG 1 cut(s) 384
BstUI CGCG 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 301, 391
BstV2I GAAGAC 2 cut(s) 40, 83
Bsu15I ATCGAT 1 cut(s) 477
BsuRI GGCC 1 cut(s) 738
BsuTUI ATCGAT 1 cut(s) 477
BtsCI GGATG 1 cut(s) 694
Cac8I GCNNGC 1 cut(s) 736
Cfr13I GGNCC 2 cut(s) 164, 455
Cfr9I CCCGGG 1 cut(s) 239
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 1 cut(s) 74
Csp6I GTAC 3 cut(s) 244, 291, 431
CviAII CATG 7 cut(s) 184, 438, 451, 573, 610, 711, 723
CviQI GTAC 3 cut(s) 244, 291, 431
DdeI CTNAG 1 cut(s) 588
DpnI GATC 1 cut(s) 480
DpnII GATC 1 cut(s) 478
DraI TTTAAA 1 cut(s) 601
EaeI YGGCCR 1 cut(s) 736
Ecl136II GAGCTC 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 164, 455
Eco53kI GAGCTC 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 239
EcoICRI GAGCTC 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 15, 160
EcoT22I ATGCAT 1 cut(s) 566
EcoT38I GRGCYC 1 cut(s) 142
FaeI CATG 7 cut(s) 187, 441, 454, 576, 613, 714, 726
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FaqI GGGAC 1 cut(s) 468
FatI CATG 7 cut(s) 183, 437, 450, 572, 609, 710, 722
FauI CCCGC 1 cut(s) 500
Fnu4HI GCNGC 3 cut(s) 315, 405, 739
FokI GGATG 1 cut(s) 701
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 3 cut(s) 315, 405, 739
FspBI CTAG 2 cut(s) 294, 536
GluI GCNGC 3 cut(s) 315, 405, 739
HaeIII GGCC 1 cut(s) 738
HapII CCGG 5 cut(s) 196, 240, 683, 742, 756
HgaI GACGC 1 cut(s) 74
Hin1II CATG 7 cut(s) 187, 441, 454, 576, 613, 714, 726
HindIII AAGCTT 2 cut(s) 269, 368
HinfI GANTC 2 cut(s) 227, 494
HpaII CCGG 5 cut(s) 196, 240, 683, 742, 756
HphI GGTGA 1 cut(s) 637
Hpy166II GTNNAC 2 cut(s) 469, 578
Hpy188I TCNGA 9 cut(s) 36, 118, 130, 144, 226, 232, 301, 591, 671
Hpy188III TCNNGA 3 cut(s) 170, 196, 536
Hpy8I GTNNAC 2 cut(s) 469, 578
Hpy99I CGWCG 3 cut(s) 40, 90, 176
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 3 cut(s) 187, 419, 564
HpyF10VI GCNNNNNNNGC 2 cut(s) 367, 386
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 7 cut(s) 187, 441, 454, 576, 613, 714, 726
Kpn2I TCCGGA 1 cut(s) 195
Kzo9I GATC 1 cut(s) 478
LmnI GCTCC 4 cut(s) 137, 145, 385, 682
Lsp1109I GCAGC 2 cut(s) 301, 391
LweI GCATC 1 cut(s) 301
MaeI CTAG 2 cut(s) 294, 536
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 424
MalI GATC 1 cut(s) 480
MboI GATC 1 cut(s) 478
MboII GAAGA 7 cut(s) 14, 17, 40, 83, 172, 473, 551
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 6 cut(s) 109, 203, 351, 398, 665, 746
MluI ACGCGT 1 cut(s) 83
MmeI TCCRAC 6 cut(s) 59, 78, 129, 204, 229, 279
MnlI CCTC 7 cut(s) 39, 42, 112, 177, 205, 354, 679
Mph1103I ATGCAT 1 cut(s) 566
MroI TCCGGA 1 cut(s) 195
MseI TTAA 7 cut(s) 74, 123, 267, 567, 600, 702, 749
MslI CAYNNNNRTG 1 cut(s) 442
MspI CCGG 5 cut(s) 196, 240, 683, 742, 756
MspR9I CCNGG 5 cut(s) 17, 162, 240, 241, 757
MvaI CCWGG 2 cut(s) 17, 162
MvnI CGCG 1 cut(s) 85
MwoI GCNNNNNNNGC 2 cut(s) 367, 386
NciI CCSGG 3 cut(s) 240, 241, 757
NdeII GATC 1 cut(s) 478
NlaIII CATG 7 cut(s) 187, 441, 454, 576, 613, 714, 726
NlaIV GGNNCC 2 cut(s) 456, 633
NsiI ATGCAT 1 cut(s) 566
NspI RCATGY 3 cut(s) 441, 576, 726
NspV TTCGAA 1 cut(s) 322
PciI ACATGT 2 cut(s) 437, 572
PfeI GAWTC 2 cut(s) 227, 494
PkrI GCNGC 3 cut(s) 316, 406, 740
PpuMI RGGWCCY 1 cut(s) 164
PscI ACATGT 2 cut(s) 437, 572
PshAI GACNNNNGTC 1 cut(s) 44
PshBI ATTAAT 1 cut(s) 567
Psp124BI GAGCTC 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 164
Psp6I CCWGG 2 cut(s) 15, 160
PspGI CCWGG 2 cut(s) 15, 160
PspN4I GGNNCC 2 cut(s) 456, 633
PspPI GGNCC 2 cut(s) 164, 455
PspPPI RGGWCCY 1 cut(s) 164
RsaI GTAC 3 cut(s) 245, 292, 432
RsaNI GTAC 3 cut(s) 244, 291, 431
RseI CAYNNNNRTG 1 cut(s) 442
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 7 cut(s) 74, 123, 267, 567, 600, 702, 749
SatI GCNGC 3 cut(s) 315, 405, 739
Sau3AI GATC 1 cut(s) 478
Sau96I GGNCC 2 cut(s) 164, 455
ScrFI CCNGG 5 cut(s) 17, 162, 240, 241, 757
SduI GDGCHC 1 cut(s) 142
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 322
SinI GGWCC 2 cut(s) 164, 455
SmaI CCCGGG 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 442
Sse9I AATT 6 cut(s) 109, 203, 351, 398, 665, 746
SsiI CCGC 4 cut(s) 317, 347, 507, 739
SspMI CTAG 2 cut(s) 294, 536
SstI GAGCTC 1 cut(s) 142
StyD4I CCNGG 5 cut(s) 15, 160, 238, 239, 755
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 249
TaqI TCGA 4 cut(s) 171, 210, 322, 477
TaqII GACCGA 1 cut(s) 472
TasI AATT 6 cut(s) 109, 203, 351, 398, 665, 746
TatI WGTACW 1 cut(s) 430
TauI GCSGC 1 cut(s) 741
TfiI GAWTC 2 cut(s) 227, 494
Tru1I TTAA 7 cut(s) 74, 123, 267, 567, 600, 702, 749
Tru9I TTAA 7 cut(s) 74, 123, 267, 567, 600, 702, 749
TseI GCWGC 2 cut(s) 314, 404
TspDTI ATGAA 3 cut(s) 147, 172, 720
TspMI CCCGGG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 164, 455
VspI ATTAAT 1 cut(s) 567
XapI RAATTY 1 cut(s) 665
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 3 cut(s) 441, 576, 726
XmaI CCCGGG 1 cut(s) 239
XspI CTAG 2 cut(s) 294, 536
Zsp2I ATGCAT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.