RLG00000023006

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002743
Physical Location & Seq
Reverse (-)
16595464 .. 16598864
3401 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023006

Sequence Viewer

Length: 813 bp
ATGAAGGCTTTGGCTGTTGTTTGCCCCCCAAGAATTTCCTTCTCCTGCTCCTCATGGCAACAACGACACCTTCGGTTTCGGCCCAAACCGAAATCCCAGCTCGGAAACTCACGGTTTCGGGTCTCGTCGTGTTTGATAAAAGAGGGAGAAAGCGAAGAATCAGAAAGGCAGAAATACAAAGGTGGAGTGGTGGATGAGTTGTTGGTGAATGTGTTCAGGAACAAAATGGTTAAACAAGTGGGATGGGATTCCGATAAGCCGGGATACGATGGACTGATTGAAGTTGCCAACCGGATGATGCTTAACAACCCAACAAATTCTCATACTAAAGAAGCTGCAGTCCGAGTTCTGATATCTTTGTTTCCACCATTCCTGTTAGAACTGTATAAACTGCTTATAGCTCCTATACAAGGCGGAAAAATAGCCGCCCTCATGGTTGCAAGGGTGACTGCAATCAGTTGTCAATGGCTCATGGGCCCCTGCACTGTTAATTCTGTCGACCTACCCGATGGGAATTCATGGACTAGTGGGGTGTTTGTCGAGAAATGCAAGTACTTGGAGGAAAGCAAATGTGTAGGGATCTGTGTCAATACTTGTAAGCTTCCAACTCAGTCTTTTTTCAAGGATTACATGGGTGTCCCTTTACTGATGGAACCGAACTTCAACGACTATAGCTGCCAGTTTAAATTCGGGGTCCTTCCTCCACTTCCCGAAGATGACACCGCTCTCAAGGAGCCTTGCTTAGCCATATGCCCTAATGCTGCCCGAAGAAGAGAAAGAAACATTAATGTTGAGCAGTGTCCCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

271

Amino Acids

30.3

Weight (kDa)

8.84

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D27-like_C PF13225 156 - 237 3.5e-35 Beta-carotene isomerase D27-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 725
AccI GTMKAC 1 cut(s) 498
AciI CCGC 3 cut(s) 414, 426, 723
AclWI GGATC 1 cut(s) 587
AcsI RAATTY 4 cut(s) 33, 316, 514, 686
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 281, 622, 664
AhlI ACTAGT 1 cut(s) 524
AluBI AGCT 5 cut(s) 100, 335, 401, 601, 675
AluI AGCT 5 cut(s) 100, 335, 401, 601, 675
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 1 cut(s) 587
AoxI GGCC 2 cut(s) 80, 475
ApaI GGGCCC 1 cut(s) 479
ApeKI GCWGC 3 cut(s) 335, 675, 761
ApoI RAATTY 4 cut(s) 33, 316, 514, 686
AseI ATTAAT 1 cut(s) 786
Asp700I GAANNNNTTC 1 cut(s) 212
AspS9I GGNCC 4 cut(s) 81, 475, 476, 694
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 2 cut(s) 217, 457
AvaII GGWCC 1 cut(s) 694
BaeGI GKGCMC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 479
BbvI GCAGC 3 cut(s) 322, 662, 748
BccI CCATC 4 cut(s) 237, 263, 503, 643
BciVI GTATCC 1 cut(s) 257
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 127
BcuI ACTAGT 1 cut(s) 524
BfaI CTAG 1 cut(s) 525
BfmI CTRYAG 2 cut(s) 336, 670
BfuI GTATCC 1 cut(s) 257
BisI GCNGC 4 cut(s) 336, 426, 676, 762
BlpI GCTNAGC 1 cut(s) 742
BlsI GCNGC 4 cut(s) 337, 427, 677, 763
BmcAI AGTACT 1 cut(s) 554
Bme1390I CCNGG 1 cut(s) 261
Bme18I GGWCC 1 cut(s) 694
BmgT120I GGNCC 4 cut(s) 81, 475, 476, 694
BmiI GGNNCC 5 cut(s) 477, 478, 654, 695, 735
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 742
BpuEI CTTGAG 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 127
BsaJI CCNNGG 1 cut(s) 804
BsaWI WCCGGW 1 cut(s) 291
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 679
BseDI CCNNGG 1 cut(s) 804
BseGI GGATG 3 cut(s) 199, 248, 300
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 623
BseNI ACTGG 1 cut(s) 679
BseRI GAGGAG 1 cut(s) 40
BseSI GKGCMC 1 cut(s) 479
BseXI GCAGC 3 cut(s) 322, 662, 748
BseYI CCCAGC 1 cut(s) 96
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 2 cut(s) 82, 477
BsiSI CCGG 2 cut(s) 260, 292
BslFI GGGAC 2 cut(s) 623, 786
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 1 cut(s) 127
BsmFI GGGAC 2 cut(s) 623, 786
BsnI GGCC 2 cut(s) 82, 477
Bso31I GGTCTC 1 cut(s) 127
Bsp120I GGGCCC 1 cut(s) 475
Bsp1286I GDGCHC 1 cut(s) 479
Bsp143I GATC 1 cut(s) 579
Bsp1720I GCTNAGC 1 cut(s) 742
BspACI CCGC 3 cut(s) 414, 426, 723
BspANI GGCC 2 cut(s) 82, 477
BspCNI CTCAG 1 cut(s) 622
BspLI GGNNCC 5 cut(s) 477, 478, 654, 695, 735
BspMAI CTGCAG 1 cut(s) 340
BspPI GGATC 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 127
BsrBI CCGCTC 1 cut(s) 725
BsrI ACTGG 1 cut(s) 679
BssECI CCNNGG 1 cut(s) 804
BssMI GATC 1 cut(s) 579
BssT1I CCWWGG 1 cut(s) 804
Bst4CI ACNGT 3 cut(s) 114, 384, 487
Bst6I CTCTTC 1 cut(s) 766
BstDEI CTNAG 2 cut(s) 609, 742
BstENI CCTNNNNNAGG 1 cut(s) 408
BstF5I GGATG 3 cut(s) 199, 248, 300
BstKTI GATC 1 cut(s) 582
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 1 cut(s) 579
BstSCI CCNGG 1 cut(s) 259
BstSFI CTRYAG 2 cut(s) 336, 670
BstSLI GKGCMC 1 cut(s) 479
BstV1I GCAGC 3 cut(s) 322, 662, 748
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 2 cut(s) 82, 477
BtsCI GGATG 3 cut(s) 199, 248, 300
BtsI GCAGTG 1 cut(s) 803
BtsIMutI CAGTG 2 cut(s) 483, 803
Cfr13I GGNCC 4 cut(s) 81, 475, 476, 694
Csp6I GTAC 1 cut(s) 553
CviAII CATG 5 cut(s) 54, 433, 472, 519, 631
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 2 cut(s) 609, 742
DpnI GATC 1 cut(s) 581
DpnII GATC 1 cut(s) 579
DraI TTTAAA 1 cut(s) 685
Eam1104I CTCTTC 1 cut(s) 766
EarI CTCTTC 1 cut(s) 766
EciI GGCGGA 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 804
Eco24I GRGCYC 1 cut(s) 479
Eco31I GGTCTC 1 cut(s) 127
Eco32I GATATC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 694
EcoNI CCTNNNNNAGG 1 cut(s) 408
EcoO109I RGGNCCY 2 cut(s) 476, 694
EcoRI GAATTC 1 cut(s) 514
EcoRV GATATC 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 804
EcoT38I GRGCYC 1 cut(s) 479
ErhI CCWWGG 1 cut(s) 804
FaeI CATG 5 cut(s) 57, 436, 475, 522, 634
FaqI GGGAC 2 cut(s) 623, 786
FatI CATG 5 cut(s) 53, 432, 471, 518, 630
FauNDI CATATG 1 cut(s) 749
FblI GTMKAC 1 cut(s) 498
Fnu4HI GCNGC 4 cut(s) 336, 426, 676, 762
FokI GGATG 3 cut(s) 206, 255, 307
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 4 cut(s) 336, 426, 676, 762
FspBI CTAG 1 cut(s) 525
GluI GCNGC 4 cut(s) 336, 426, 676, 762
GsaI CCCAGC 1 cut(s) 100
HaeIII GGCC 2 cut(s) 82, 477
HapII CCGG 2 cut(s) 260, 292
Hin1II CATG 5 cut(s) 57, 436, 475, 522, 634
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 2 cut(s) 158, 248
HpaII CCGG 2 cut(s) 260, 292
HphI GGTGA 2 cut(s) 217, 457
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 5 cut(s) 104, 163, 253, 344, 351
Hpy188III TCNNGA 3 cut(s) 217, 541, 710
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 3 cut(s) 49, 80, 707
HpyCH4III ACNGT 3 cut(s) 114, 384, 487
HpyCH4V TGCA 5 cut(s) 338, 440, 452, 483, 549
HpyF3I CTNAG 2 cut(s) 609, 742
Hsp92II CATG 5 cut(s) 57, 436, 475, 522, 634
Kzo9I GATC 1 cut(s) 579
LmnI GCTCC 3 cut(s) 53, 406, 733
LpnPI CCDG 8 cut(s) 58, 110, 202, 273, 305, 386, 493, 692
Lsp1109I GCAGC 3 cut(s) 322, 662, 748
LweI GCATC 1 cut(s) 288
MaeI CTAG 1 cut(s) 525
MaeIII GTNAC 1 cut(s) 445
MalI GATC 1 cut(s) 581
MbiI CCGCTC 1 cut(s) 725
MboI GATC 1 cut(s) 579
MboII GAAGA 4 cut(s) 167, 725, 780, 783
MflI RGATCY 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 479
MluCI AATT 5 cut(s) 33, 316, 490, 514, 686
MmeI TCCRAC 1 cut(s) 629
MnlI CCTC 5 cut(s) 61, 136, 440, 553, 711
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 5 cut(s) 231, 303, 489, 684, 786
MspI CCGG 2 cut(s) 260, 292
MspR9I CCNGG 1 cut(s) 261
NciI CCSGG 1 cut(s) 261
NdeI CATATG 1 cut(s) 749
NdeII GATC 1 cut(s) 579
NlaIII CATG 5 cut(s) 57, 436, 475, 522, 634
NlaIV GGNNCC 5 cut(s) 477, 478, 654, 695, 735
NmuCI GTSAC 1 cut(s) 445
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 2 cut(s) 158, 248
PkrI GCNGC 4 cut(s) 337, 427, 677, 763
PpuMI RGGWCCY 1 cut(s) 694
PshBI ATTAAT 1 cut(s) 786
Psp5II RGGWCCY 1 cut(s) 694
PspFI CCCAGC 1 cut(s) 96
PspN4I GGNNCC 5 cut(s) 477, 478, 654, 695, 735
PspOMI GGGCCC 1 cut(s) 475
PspPI GGNCC 4 cut(s) 81, 475, 476, 694
PspPPI RGGWCCY 1 cut(s) 694
PstI CTGCAG 1 cut(s) 340
PsuI RGATCY 1 cut(s) 579
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
SalI GTCGAC 1 cut(s) 497
SaqAI TTAA 5 cut(s) 231, 303, 489, 684, 786
SatI GCNGC 4 cut(s) 336, 426, 676, 762
Sau3AI GATC 1 cut(s) 579
Sau96I GGNCC 4 cut(s) 81, 475, 476, 694
ScaI AGTACT 1 cut(s) 554
ScrFI CCNGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 479
SetI ASST 8 cut(s) 72, 102, 184, 337, 403, 504, 603, 677
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 2 cut(s) 336, 670
SinI GGWCC 1 cut(s) 694
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SpeI ACTAGT 1 cut(s) 524
Sse9I AATT 5 cut(s) 33, 316, 490, 514, 686
SsiI CCGC 3 cut(s) 414, 426, 723
SspMI CTAG 1 cut(s) 525
StyD4I CCNGG 1 cut(s) 259
StyI CCWWGG 1 cut(s) 804
TaaI ACNGT 3 cut(s) 114, 384, 487
TaqI TCGA 2 cut(s) 498, 540
TasI AATT 5 cut(s) 33, 316, 490, 514, 686
TatI WGTACW 1 cut(s) 552
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 2 cut(s) 158, 248
Tru1I TTAA 5 cut(s) 231, 303, 489, 684, 786
Tru9I TTAA 5 cut(s) 231, 303, 489, 684, 786
TscAI CASTG 2 cut(s) 490, 803
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 3 cut(s) 335, 675, 761
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 2 cut(s) 17, 507
TspRI CASTG 2 cut(s) 490, 803
VpaK11BI GGWCC 1 cut(s) 694
VspI ATTAAT 1 cut(s) 786
XagI CCTNNNNNAGG 1 cut(s) 408
XapI RAATTY 4 cut(s) 33, 316, 514, 686
XmiI GTMKAC 1 cut(s) 498
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 1 cut(s) 525
ZrmI AGTACT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.