pycom09g19180

Domain of unknown function (DUF4033)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002743
Physical Location & Seq
Reverse (-)
19942689 .. 19943410
722 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g19180.3

Sequence Viewer

Length: 387 bp
ATGGTTGCAAGGGTGACTGCAATTACTTGTCAATGGCTTATGGGTCCTTGCACAGTAAACTCAGTCGACTTACCAGACGGGACTTCATGGAACAGTGGGGTGTTTGTAGAGAAATGCAAGTACTTGGAAGAAAGCAAATGCGTCGGTATCTGTCTGAATACTTGCAAGCTTCCCACTCAGGCTTTCATCAAAGATTATATGGGCGTTCCGTTAGTGATGGAGCCGAACTTCGTCGATTACAGCTGCCAGTTTAAATTCGGTGTCCTCCCCCCACTTCCTGAAGATGATGCCACTCTCAAAGAACCCTGCTTGGAAATCTGCCCTAACGCCACTCGACGGAGAGAAATCGCCAGAACCATTAACGTCGAGAAATGTCCCAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4033

Protein Analysis

129

Amino Acids

14.28

Weight (kDa)

5.46

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 66
AcsI RAATTY 1 cut(s) 254
AcuI CTGAAG 1 cut(s) 300
AfaI GTAC 1 cut(s) 122
AfiI CCNNNNNNNGG 1 cut(s) 336
AluBI AGCT 2 cut(s) 169, 243
AluI AGCT 2 cut(s) 169, 243
ApeKI GCWGC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 254
AspS9I GGNCC 1 cut(s) 44
AsuHPI GGTGA 1 cut(s) 25
AvaII GGWCC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 230
BccI CCATC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BisI GCNGC 1 cut(s) 244
BlsI GCNGC 1 cut(s) 245
BmcAI AGTACT 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 2 cut(s) 45, 222
BmsI GCATC 1 cut(s) 277
BsaJI CCNNGG 1 cut(s) 378
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse1I ACTGG 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 336
BseMII CTCAG 2 cut(s) 75, 191
BseNI ACTGG 1 cut(s) 247
BseXI GCAGC 1 cut(s) 230
BslFI GGGAC 2 cut(s) 94, 360
BslI CCNNNNNNNGG 1 cut(s) 336
BsmFI GGGAC 2 cut(s) 94, 360
BspCNI CTCAG 2 cut(s) 74, 190
BspLI GGNNCC 2 cut(s) 45, 222
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 378
Bst4CI ACNGT 2 cut(s) 55, 95
BstC8I GCNNGC 1 cut(s) 167
BstDEI CTNAG 2 cut(s) 61, 177
BstV1I GCAGC 1 cut(s) 230
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 44
CseI GACGC 1 cut(s) 130
Csp6I GTAC 1 cut(s) 121
CviAII CATG 2 cut(s) 87, 384
CviJI RGCY 5 cut(s) 37, 169, 182, 223, 243
CviKI_1 RGCY 5 cut(s) 37, 169, 182, 223, 243
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 2 cut(s) 61, 177
DraI TTTAAA 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 378
Eco47I GGWCC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 44
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 2 cut(s) 90, 387
FaiI YATR 5 cut(s) 41, 88, 198, 200, 385
FaqI GGGAC 2 cut(s) 94, 360
FatI CATG 2 cut(s) 86, 383
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 244
GluI GCNGC 1 cut(s) 244
HgaI GACGC 1 cut(s) 130
Hin1II CATG 2 cut(s) 90, 387
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 1 cut(s) 167
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 2 cut(s) 58, 67
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 278, 367
Hpy8I GTNNAC 2 cut(s) 58, 67
Hpy99I CGWCG 4 cut(s) 146, 236, 339, 368
HpyCH4III ACNGT 2 cut(s) 55, 95
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 5 cut(s) 8, 20, 51, 117, 165
HpyF3I CTNAG 2 cut(s) 61, 177
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 2 cut(s) 90, 387
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 6 cut(s) 87, 164, 260, 291, 319, 364
Lsp1109I GCAGC 1 cut(s) 230
LweI GCATC 1 cut(s) 277
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 13
MboII GAAGA 2 cut(s) 140, 293
MluCI AATT 2 cut(s) 21, 254
MnlI CCTC 1 cut(s) 275
MseI TTAA 2 cut(s) 252, 360
MspA1I CMGCKG 1 cut(s) 243
NlaIII CATG 2 cut(s) 90, 387
NlaIV GGNNCC 2 cut(s) 45, 222
NmuCI GTSAC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 245
PpuMI RGGWCCY 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 45, 222
PspPI GGNCC 1 cut(s) 44
PspPPI RGGWCCY 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 243
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SalI GTCGAC 1 cut(s) 65
SaqAI TTAA 2 cut(s) 252, 360
SatI GCNGC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 44
ScaI AGTACT 1 cut(s) 122
SetI ASST 3 cut(s) 171, 245, 366
SfaNI GCATC 1 cut(s) 277
SinI GGWCC 1 cut(s) 44
Sse9I AATT 2 cut(s) 21, 254
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 2 cut(s) 55, 95
TaiI ACGT 1 cut(s) 366
TaqI TCGA 4 cut(s) 66, 234, 334, 366
TasI AATT 2 cut(s) 21, 254
TatI WGTACW 1 cut(s) 120
Tru1I TTAA 2 cut(s) 252, 360
Tru9I TTAA 2 cut(s) 252, 360
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 1 cut(s) 243
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 2 cut(s) 75, 175
TspGWI ACGGA 2 cut(s) 198, 352
TspRI CASTG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 44
XapI RAATTY 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 66
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.